*4.10. iN Morphology Analysis*

iPSC-iNs were generated following the protocol described in [**?** ]. Lentiviral GFP+ and unlabeled iNs were mixed in a proportion of 1:6 in order to assess morphology of individual neurons. Images were directly acquired using a BX61 fluorescence microscope (Olympus, Tokyo, Japan).


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MI. Induction of Fos-like immunoreactivity (Fos-li) and stimulation of feeding by 2,5-anhydro-D-mannitol (2,5-AM) require the vagus nerve. Brain Research. 1994;**646**(1):53-64. DOI: 10.1016/0006-8993(94)90057-4

nlm.2006.09.009

**100**

*Edited by Clémentine Bosch-Bouju, Sophie Layé and Véronique Pallet*

Nutrition is an environmental factor modulating physiology throughout life and especially brain function. Nutrients in the brain can either fuel brain cells, contribute to tissue architecture, or initiate signaling pathways through their derivatives. Nutrients ultimately participate in brain development, cognitive and emotional behaviors, and can influence the susceptibility to develop brain pathologies.

This book is a selection of current research on the impact of diet on brain function. Chapters include the role of lipids and glucose on the brain, nutrition and autophagy, and consequences of enteral feeding on brain–gut interactions.

Taken together, this book targets all scientists, clinicians, teachers, and students eager to learn more about the impact of nutrition on brain function.

Published in London, UK © 2019 IntechOpen © maksime / iStock

Feed Your Mind - How Does Nutrition Modulate Brain Function throughout Life?

Feed Your Mind

How Does Nutrition Modulate

Brain Function throughout Life?

*Edited by Clémentine Bosch-Bouju,*

*Sophie Layé and Véronique Pallet*