**6. Conclusions and future prospects**

Biofortification is a promising, cost-effective, agricultural strategy to improve the nutritional status of the world's undernourished populations. Strategies for biofortification based on crop breeding, targeted genetic manipulation, and/ or mineral fertiliser application have great potential to address human mineral malnutrition [70–72]. Developing biofortified food crops with improved nutrient content such as increased content of iron, zinc, Se, and provitamin A provides adequate levels of these and other such micronutrients that are often lacking in developed and developing diets. International initiatives, such as the CGIAR centres in collaboration with HarvestPlus and national initiatives, serve as pillars for achieving these objectives. These efforts have resulted in crops with the potential to increase both quantities and bioavailability of essential mineral elements in human diets, particularly in elementary cereal crops such as rice, wheat, maize, cassava, beans, and sweet potatoes. However, crop biofortification is a challenging task. Collaboration between plant breeders, nutritionists, genetic engineers, and molecular biologists is essential to achieving this. Breeding approaches are generalised and easy to accept and have been used to improve food nutritional qualities sustainably. Although greater emphasis is placed on molecular breeding-based approaches of which success rates are much higher as transgenically fortified crop plants, it faces

**47**

presented in **Figure 3**.

**Figure 3.**

*consumers.*

**Conflict of interest**

The authors declare no conflict of interest.

*Breeding for Biofortification Traits in Rice: Means to Eradicate Hidden Hunger*

hurdles due to consumer acceptance and costly and time-consuming regulatory approval processes adopted by different countries. Biofortified crops have a very bright future in addition to these challenges, as they have the potential to eliminate micronutrient malnutrition among billions of poor people, particularly in developing countries. Overall developmental process of the biofortified rice variety are

*Flowchart showing development and release of a biofortified variety and its acceptance by farmers and*

*DOI: http://dx.doi.org/10.5772/intechopen.91144*

*Breeding for Biofortification Traits in Rice: Means to Eradicate Hidden Hunger DOI: http://dx.doi.org/10.5772/intechopen.91144*

#### **Figure 3.**

*Agronomy - Climate Change and Food Security*

Phytic acid is an essential constituent in staple foods like legume and cereals, which has been of much concern [57]. In addition to its beneficial effect on human health, it has some anticancer and antioxidant functions and prevents coronary disease, and it is well known that phytic acid acts as strong chelating agent of mineral nutrients such as Ca, Zn, and Fe [65]. It has been seen that due to the presence of complex of phytic acid, in the form of phytate, there is a significant reduction found in bioavailability of nutrient elements [66]. It seems reasonable to control phytic acid contents in edible parts of crops to a level in which the medical and health functions of the food may be

maintained and bioavailability of minerals is not much altered [67].

the accurate evaluation of rice germplasm for phytic acid content.

Liu et al. [68] assayed 72 cultivars for protein content and phytic acid and reported a wide range for phytic acid ranging from 0.685 to 1.03%, with an average of 0.873%. Interestingly, grain phytic acid and protein content were not correlated, which suggests the possibility of breeding rice for phytic acid and high protein content. Furthermore, they also reported a significant effect of varieties, locations, and their interactions on phytic acid content, with the location having the most considerable impact which suggests the necessity of multi-environment trials for

Although sufficient genetic variation for phytic acid has been reported in various studies [68, 69], unfortunately, only one study has been conducted to map the QTLs for phytic acid in rice [18]. Stangoulis et al. [18] identified two QTLs explaining 15.4–24.3% of PVE for grain phytates from an IR64 × Azucena double haploid population. One common QTL for phytate and total P concentrations on chromosome 5 with the (high concentration) allele contributed from Azucena was identified. Furthermore, it was reported that Fe, Zn, and Mn contents in grains have different genetic regulation because the QTLs of phytate were not located on the same chromosomal regions as those found for Fe, Zn, and Mn [18]. So, there is a great possibility to find segregants having a low level of phytic acid and high level of Fe, Zn, and Mn content. Use of molecular marker in the breeding and selection to

reduce grain phytic acid and improving the nutritional value of cereal grains.

in collaboration with HarvestPlus and national initiatives, serve as pillars for achieving these objectives. These efforts have resulted in crops with the potential to increase both quantities and bioavailability of essential mineral elements in human diets, particularly in elementary cereal crops such as rice, wheat, maize, cassava, beans, and sweet potatoes. However, crop biofortification is a challenging task. Collaboration between plant breeders, nutritionists, genetic engineers, and molecular biologists is essential to achieving this. Breeding approaches are generalised and easy to accept and have been used to improve food nutritional qualities sustainably. Although greater emphasis is placed on molecular breeding-based approaches of which success rates are much higher as transgenically fortified crop plants, it faces

Biofortification is a promising, cost-effective, agricultural strategy to improve the nutritional status of the world's undernourished populations. Strategies for biofortification based on crop breeding, targeted genetic manipulation, and/ or mineral fertiliser application have great potential to address human mineral malnutrition [70–72]. Developing biofortified food crops with improved nutrient content such as increased content of iron, zinc, Se, and provitamin A provides adequate levels of these and other such micronutrients that are often lacking in developed and developing diets. International initiatives, such as the CGIAR centres

**6. Conclusions and future prospects**

**5. Phytic acid**

**46**

*Flowchart showing development and release of a biofortified variety and its acceptance by farmers and consumers.*

hurdles due to consumer acceptance and costly and time-consuming regulatory approval processes adopted by different countries. Biofortified crops have a very bright future in addition to these challenges, as they have the potential to eliminate micronutrient malnutrition among billions of poor people, particularly in developing countries. Overall developmental process of the biofortified rice variety are presented in **Figure 3**.


*Review* **Supramolecular Interactions in Hybrid Polylactide Blends—The Structures, Mechanisms and Properties**

## **Anna Kowalewska \* and Maria Nowacka**

Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łód ´z, Poland<EMAIL_ADDRESS>
**\*** Correspondence<EMAIL_ADDRESS>Tel.: +48-42-680-3350

Academic Editor: Sylvain Caillol Received: 19 June 2020; Accepted: 20 July 2020; Published: 23 July 2020

**Abstract:** The conformation of polylactide (PLA) chains can be adjusted by supramolecular interactions (the formation of hydrogen bonds or host-guest complexes) with appropriate organic molecules. The structures formed due to those intermolecular interactions may act as crystal nuclei in the PLA matrix ("soft templating"). In this review, the properties of several supramolecular nucleating systems based on synthetic organic nucleators (arylamides, hydrazides, and 1,3:2,4-dibenzylidene-d-sorbitol) are compared to those achieved with biobased nucleating agents (orotic acid, humic acids, fulvic acids, nanocellulose, and cyclodextrins) that can also improve the mechanical properties of PLA. The PLA nanocomposites containing both types of nucleating agents/additives are discussed and evaluated in the context of their biomedical applicability.

**Keywords:** polylactide; composites; supramolecular interactions; crystallization


*Edited by Fabio A. Marchi, Priscila D.R. Cirillo and Elvis C. Mateo*

The large potential of RNA sequencing and other "omics" techniques has contributed to the production of a huge amount of data pursuing to answer many different questions that surround the science's great unknowns. This book presents an overview about powerful and cost-efficient methods for a comprehensive analysis of RNA-Seq data, introducing and revising advanced concepts in data analysis using the most current algorithms. A holistic view about the entire context where transcriptome is inserted is also discussed here encompassing biological areas with remarkable technological advances in the study of systems biology, from microorganisms to precision medicine.

Photo by bobuz / iStock

Applications of RNA-Seq and Omics Strategies - From Microorganisms to Human Health

Applications of RNA-Seq

and Omics Strategies

From Microorganisms to Human Health

*Edited by Fabio A. Marchi,*

*Priscila D.R. Cirillo and Elvis C. Mateo*


**2. Equity of Educational Opportunities in China from the Human Capability Approach**

As an important social issue in China, EE is commonly associated with topics like economic growth, social solidarity, social equality and justice, and draws wide attention. Since the Tenth National Five-Year Plan for Education Development released in 2001 proposed "EE" as a basic education policy for the first time, China has made documented progress in promoting EE. However, due to China's huge population, social stratification and vastness in territory, the development of EE in China is arduous. Although this kind of extensive resource redistribution among different regions and population groups is conducive to achieving relatively fair results at the macro level, it masks many practical problems and dilemmas. In particular, the individual experience of fairness and the development of specific abilities of vulnerable groups are actually invisible with the extensive policies. According to the theory of human capability development, the focus of EE promotion should shift from extensiveness to fineness, from the equality of resources to the equality of human capability. In other words, circumstances dictate that the development of EE in China needs to move beyond macro control, taking into consideration the micro- and fine-grained factors, and consider the equitable development of individual capability.

At the beginning of each year, the Ministry of Education of the People's Republic of China issues the Key Points of Work of the Ministry of Education as a guide to the education work for the year. A textual analysis documents from 2012 to 2022 shows that "equity in education" is a core task every year, and "educational equity" still aims at promoting equality of educational opportunities and balanced allocation of educational resources (see Table 2).

**Table 2.** Key points of work of the Ministry of Education from 2012 to 2022 for the purpose of "educational equity".

Source: Key Points of Work of the Ministry of Education (2012–2022) http://www.moe.gov.cn/jyb\_sjzl/moe\_164/ (accessed on 25 February 2023).

Although some scholars point out that China's EE has entered a stage of "equal quality" from the old stage of "equal opportunity", in terms of practices, there are complications. These EE-related problems mainly exist in disadvantaged groups such as rural populations, populations in poverty-stricken areas, ethnic minority populations, left-behind children, and children who are living with their migrant parents. For a long time, disadvantaged groups have been the main target of EE, but they are still faced with the real problem of being deprived of capability.

Problems related to ethnic minority groups: Preferential policies for ethnic minority education in China can be divided into four categories: special policies for running schools (classes) for inland ethnic groups; special policies for senior high school entrance examination and college entrance examination; special policies for university enrollment; and special policies for ethnic preparatory classes in colleges and universities. Although these policies are aimed at enhancing the education opportunities for minority students, they are not adequate. According to an empirical survey conducted in 2013, the average length of education of China's ethnic minority population is shorter than the national average, and ethnic minority women are the group most affected by EI in particular [12]. Influenced by their social custom, China's ethnic minorities as a whole do not pay sufficient attention to education compared to the Han, and their motivation for education is not as strong. Worse still, the current teaching system is dominated by the Han culture, causing learning obstacles for ethnic minorities [13]. At the same time, the preferential education policy gives preferential treatment to all students with the "minority identity", which has caused a lot of new problems, such as "hitchhiking" by the dominant social strata in the ethnic groups and falsification of ethnic identity. In practice, therefore, the preferential education policy has become an "educational privilege" for some people and stimulated more inequality [14].

Problems related to migrant children: China's current household registration (Hukou) system creates a dual social structure, exacerbating EI problems to rural migrants working in cities. Since migrant workers are rural by Hukou registration but work in the cities, it is often difficult for their children to attend urban schools. According to statistics, the floating population (non-permanent resident in a region) in China reached 376 million in 2020, including about 200 million migrant workers [15]. Although China implements a college matriculation policy for migrant children in order to meet their needs for fair education opportunities, all localities, especially developed areas, take a "high-threshold policy", which overtly deprives migrant children of their opportunity to higher education. For example, in Beijing, the conditions for migrant children to take the college entrance examination are the following: their parents must hold a valid Beijing residence permit, residence registration card or work and residence permit, have a stable residence and occupation, and pay social insurance premiums for a certain number of years continuously; and the children must have a student status in Beijing and have been attending high school for 3 years in a row. However, even if they have the above qualifications, migrant children can only apply for the entrance examination of higher vocational schools. As a result, migrant children cannot realize the life state that they have a reason to cherish through free choice, which shows a lack of capability. This includes the lack of ability to choose further studies (such as lack of academic ability and insufficient ability to pay for education), the deprivation of local opportunities for further studies, or unequal opportunities for further studies (in fact, many cities, especially Beijing, Shanghai and Guangzhou, only offer vocational education to migrant children) [16].

Problems related to urban–rural gaps: China's administrative division has three categories: Urban Areas, County and Town Areas, and Rural Areas. This is the basis of China's regional education disparity management, macro-level resource allocation, and data collection and comparison for measuring urban–rural gaps. According to this logic and the Educational Statistics Yearbook of China [17], the urban–rural gap in education has almost been eliminated. Take the number of books and digital resources in the basic education stage as an example (see Table 3). Although Urban Areas have the most books and digital resources, followed by County and Town Areas, and then by Rural Areas, the Senior high school

Junior high school

> Primary school

Quantity per student

Number of students in

Quantity per student

Number of students in

Quantity per student

Number of

Number of

Number of books

Number of books

number of students in these three types of areas are also in the same descending order. Therefore, from a per-student perspective, there is little difference in educational resources between the three types of areas; moreover, the average number of books and computers available per student in Rural Areas is even higher than that in Urban Areas and County and Town Areas. Does this mean no regional difference in education in China? Only in numbers, not from the perspective of capabilities. This is because not everyone can turn the provided educational resources into the same or similar advantages in life [8] (p .276).

(rounded) 46 0.25 36 0.16 43 0.22

(rounded) 35 0.17 37 0.15 45 0.18

school 42,030,976 40,717,741 24,504,815

(rounded) 39 0.015 23 0.0074 28 0.0084

computers 663,943,679 3,307,564 866,688,808 3,499,747 286,113,228 1,186,617

computers 949,166,676 622,984 933,357,062 301,632 696,694,344 206,818

school 19,029,366 23,733,472 6,378,055

**Table 3.** China's urban–rural gaps in terms of the number of books and digital resources for basic education (2020).

For example, schools carried out large-scale online teaching due to COVID-19 in 2020. The digital teaching crisis revealed that the actual digital resources available to urban and rural students depended on the capability of students' families, rather than the number of school computers shown in the above statistics. A survey in 2020 showed that 70.62% of students in Urban Areas and County and Town Areas used computers or tablets to attend online classes, while 74.98% of students in Rural Areas used mobile phones [18] (p. 63). Therefore, it was clear that the urban–rural gap in education was further widened due to the urban–rural gap in family capability. Admittedly, educational aid can help low-SES students to a certain extent, which is also one of the important educational policies in China. In 2014, 2018, 2020 and 2021, the Chinese government provided subsidies of RMB 11.51 billion, 18.98 billion, 16.89 billion and 16.43 billion, respectively, to ordinary high school students, and most of the funds flowed to the central and western regions where

low-SES students were concentrated (see Figure 1). However, it remains unclear to what

extent these subsidies have improved the capability of disadvantaged groups.

**Figure 1.** The number of subsidized ordinary high school students. Data comes from China National Center for Student Financial Aid. https://www.xszz.edu.cn/n85/index.html(accessed on 3 March 2023).

According to the latest statistics, the gross enrollment rate of higher education in China reached 57.8% in 2021, and the population with higher education backgrounds reached 240 million. MOE China declares, institutionalizing the famous Martin Trow Elite-Mass-Universal triptych on the development of Higher Education in a country, that China marking that the Chinese population now has a universal higher education. Although increasing university enrollment opportunities seems to be able to correct social injustice, it actually masks the inequality of opportunities for low-SES students to transform their capability into functions [9] (p. 70). This is because low-SES students cannot effectively convert resources into functions like their counterparts from more affluent background, and they have various obstacles to the realization of functions in school. This is more obvious after they start attending college. A "study on low-SES students' adaptability to campus life" shows that students from poor families have lower adaptability and longer adaptation period than urban students after entering the universities. This research samples students in China's 'World Class Universities' and 'World Class Disciplines', a token for their exceptional ability to get great scores in college entrance examinations. Despite their extraordinary academic performance, the researchers find that university students from low SES backgrounds are less "dedicated", less concentrated on learning, less interested in the discipline they learn, and introverted in interpersonal relations [19]. This means that even if low-SES students have the same academic achievements as high-SES students, there is still a big gap between them, which is caused, in essence, by the gap in capability.

Therefore, the equality of educational resources and results does not necessarily mean the equality of education. According to Nussbaum, among the three ways to promote social justice, the resource-based approach and the preference-based approach cannot solve the problem of inequality but aggravate it instead. In fact, the problem with fair allocation of resources is that different individuals have different capabilities to transform resources into functions. This includes both physical differences and social (hierarchical) differences [20] (pp. 232–233).

Therefore, the capability approach opposes the traditional view of fair distribution of resources and criticizes the traditional theory about what inputs (ideas, teachers and teaching materials) form specific opportunities to achieve the expected results (economic growth or social solidarity). The capability theory holds that an evaluation of social (including educational) arrangements must be based on people's capability, rather than the resources they can obtain or the results they can achieve [5] (pp. 2,4). Therefore, according to Sen's concept of equality, what should be equal is not resources (such as equitable allocation of education funds) or results (such as the leveling of students' qualifications), but people's capability, that is, what people can become and do [5] (p. 3).

This means that we need to evaluate educational development based on people's capabilities, accurately identify the capabilities of different groups according to their economic, cultural, capital and social status, reconstruct the EE development framework based on the full investigation and experiments according to the basic idea of expanding the capability set beyond the macro allocation of resources, and reach a more complex, diverse, individualized and refined level so as to truly enhance the ability of disadvantaged groups through education.

#### **3. Capability Building: Actionable Research Application on Educational Equity**

The capability approach has been constantly questioned since it was put forward. One of the most famous questions is "To what extent is the capability framework operable?" [21] (p. 1953). Although Nussbaum criticized Sen for not establishing a clear framework and developing the list of ten core capabilities by means of analytical philosophy, Sen himself has always opposed setting a fixed list of capabilities, advocated an engaged human development model, and emphasized the importance of public participation and dialogue in achieving valuable capabilities. This means that some form of participatory dialogue is always needed in evaluating education-related capabilities [5] (p. 12). According to Santos Mehrotra's research, it is only at the community level that the capability approach can be really practical and useful [22] (p. 306). In this sense, actionable research is one of the effective ways to solve the EI problem using the capability approach.

Actionable research, proposed by Edward St. John, aims to identify social and educational inequities and their root causes. Through actionable research, we can reflectively choose the policies and action strategies from multiple options to address these inequities. The specific steps include identifying problems, collecting data or conducting surveys, determining solutions, taking actions, evaluating effects, and revising policies and practices, and all of these steps require cooperation among researchers, policymakers and practitioners [23] (p. 147).

The capability approach is essentially an evaluation method, so in order to make this method operable, it is necessary to determine the conditions that lead to the realization of simple functions (such as being able to read and write) and complex functions (such as being able to participate in community life and having self-esteem). Embedding the capability approach into the actionable research model means that when evaluating the topics that need to be reformed in the research-based action inquiry model (AIM), researchers and practitioners need to jointly determine the conditions for individuals to realize simple functions and complex functions; evaluate whether the development of education makes it possible to realize these functions; generate problems on this basis; conduct empirical research or data collection; and further determine the generative system to expand students' capability while following quantitative and qualitative evaluation methods. However, the measurement of capability and function is the most important challenge in applying the capability approach in empirical research and specific social environment, and the pluralism of Sen's evaluation framework actually makes research, policymaking and practice more complicated [24]. In comparison, Nussbaum's view that emphasizes the necessity of a list of core and universal human capabilities and supports the establishment of a national standard for specific capabilities is more operable as the basic way for different scholars to apply Sen's framework.

It is the challenge of building a system of capabilities and the unique role of actionable research in addressing this challenge that makes it possible to apply the capability approach to deal with educational inequalities through actionable research. That is to say, one of the important ways to construct the capability framework of disadvantaged groups is to design or create a basic capability list as a reference standard based on a large number of empirical studies and discussions under a specific social background so as to identify and examine the capabilities that a certain group lacks and the specific factors thereof, to explore, develop and enhance the educational projects or methods for the identified capabilities.

Following the three methods of constructing the capability list commonly used in the world, Chinese scholars have studied China's capability lists of three aspects: "expertselected capabilities", "more complicated rules and procedures for identifying capabilities" and "listening to the voices of disadvantaged groups" [6] (p. 6). The available lists present different results (see Table 4) [25] (p.80) [26] (p.122). This shows that the construction of capability lists are different when the groups they face, the problems they try to solve and the resources they have are different. In fact, this is in line with the viewpoint of the capability approach that recognizes the heterogeneity and diversity of human beings and pays attention to group differences. It is precisely because of this that capability building has become an effective way to solve the EI problem substantially. However, the available lists lack public participation and dialogue, while the capability approach emphasizes the importance of public participation in discussions and rejects paternalistic decisions. Sen clearly pointed out that decisions on what capabilities should be chosen should not be made only by local elites or cultural experts without the participation of direct stakeholders [27] (pp. 31–32).

**Table 4.** Comparison of two capability lists in China.

Actionable research advocates the comprehensive use of three capability construction methods: analytical and philosophical construction at the academic level, empirical research on social reality, and letting disadvantaged groups speak out. The three methods used in combination will help put forward a representative list of capabilities.

However, creating a capability list is only the first step. To truly expand the capability set of disadvantaged groups, collective or organized activities are needed with the list as a reference standard. In other words, solving the problem of inequality in practice also requires cooperation among local schools, communities, governments, enterprises, foundations and other parties through public- and private-funded social networks. Taking the education of migrant children in Shanghai as an example, the city has formed an effective and refined cooperative group to solve the problem of EI for migrant children.

• Educational institutions conducted special investigations and studies. In 2011, for example, the Committee for Migrant Children's Education of the Chinese Society for the Study of Tao Xingzhi carried out a "Research on the Education of Migrant Children after Junior High School in the Context of Urbanization" and published the *Blue Book of Education of Migrant Children* in Shanghai, which pointed out the education-related problems faced by migrant workers' children in Shanghai and provided an important reference for further policy formulation.

**Figure 2.** Cooperative groups in Shanghai.

Thus, the basic model of actionable research for constructing a list of capabilities for EE begins with the construction of a list of capabilities through engaged scholarship, using the functions presented by the list of capabilities as evaluation factors to locate and analyze the reasons for the lack of viable competencies of disadvantaged groups. This is followed by an analysis of the personal and social environment of disadvantaged groups and what influences them to transform what they already have into enabling resources, thus exploring ways of enhancing viable capability sets. Finally, the list of capabilities is practiced and revised through the collaboration of individuals, families, communities, and governments (see Figure 3).

**Figure 3.** Actionable research route to build a list of capabilities.

#### **4. Case Study: The LFCF Program and Its Efforts on Educational Equity**

As mentioned above, actionable research is a principal and practical means to solve the EI problem through the capability approach. But what can be implemented specifically? To respond to this question, we further introduce our educational experiment in collaboration with the Chen Yidan Foundation.

In 2020, to promote the development of people's capability, we joined the Learner for the Future Competence Frame (LFCF) program launched by the Chen Yidan Foundation and established an education expert workshop. In the past two years, we have been carrying out research and experiments on how to re-endow learners with a subjective status. The core idea of the workshop is that education should focus on enhancing the development of student capabilities rather than emphasizing quantitative achievements, such as test scores. Therefore, a collaborative educational ecology involving students, families, schools and communities was built. The ultimate aim of the LFCF program is to develop learners with infinite growth possibilities, meaning that individuals develop sufficient viable competencies over the course of education and be able to achieve their own goals for a better life.

Referencing actionable research principles, the LFCF project is a collaborative educational dialogue and experiment with a rich hierarchy of participants, including students, parents, K-12 education practitioners, university academics, social activists and relevant government personnel, as well as a team of international scholars. The professors range from those with a Western cultural background to those who are native to China. The native professors are from several key universities in China, such as Tsinghua University, Peking University, Beijing Normal University and Zhejiang University. Participating K-12 education practitioners include both educators from regular schools, such as the Affiliated High School of Peking University, and educators from innovative schools, such as Avenues International School. Participating students and parents mainly come from the educational experiments conducted by the LFCF project. After numerous talks, seminars and workshops, we presented our final research report. "Competence and Education of the Learner for the Future" [28] was presented at the international education forum "Learning Ecosystem for the Future: Family, School, Society" on 12–13 November 2022. The two core contents of the report are a competence framework for the infinite growth of individuals and a PBL-dominated comprehensive practice system of educational grammar.

For students, the most critical step in capability building is the improvement of their ability, which is also one of the core goals of the LFCF project. In the early stage of the project, the LFCF team held a workshop and invited various stakeholders to discuss and analyze 763 literacy databases formed by 45 global literacy frameworks. Based on the scenario and social reality of Chinese students, a capability list (see Table 5) was preliminarily developed and a PBL-dominated community education model was proposed to effectively help students develop those capabilities. With this list as a benchmark, Chen Yidan Foundation launched the "Mars Rescue Plan" summer camp in the Shenzhen Mingde Experimental School in July 2021. They provided a set of PBL education courses oriented to the development of the capability list with the students who entered the camping. At the end of the camping, they showcased relevant achievements in a seminar and collected comments and suggestions on this educational activity from the participating students and their parents. Then, we further adjusted and optimized the capability list based on empirical results. After two years of refinement and re-calibration, we can say that the learners in the context of China should have five basic capabilities: exploration, critical thinking, collaboration, creativity and care. These five capabilities are what people as both individuals and part of society should develop. They transcend the social structure stratification and group differences and lead to the ultimate equality of all individuals in realizing their capabilities.

**Table 5.** Capability list of the LFCF project.

Consistent with Nussbaum's view on the necessity of updating the list of basic capabilities, the LFCF team believes that these five basic capabilities are a relatively complete but open and growing framework. At the same time, we refined 31 secondary literacy indicators and provided specific references for curriculum development and teachers' teaching planning. More importantly, to increase the practicality and effectiveness of the list, the LFCF team set up a matching comprehensive practice system—Future Educational Grammar (CMYK), an interdisciplinary meta-programming system for curriculum projects corresponding to the capability list.

We not only try to provide a framework and effective tools for educational reform, but also work with individuals, families, communities, governments and other parties, emphasizing the diversified development of educational ecology. The capability approach advocates the participatory mode, and the development of EE also depends on the participation and practice of various stakeholders. At this stage, we have flexibly applied the capability list and the CMYK practice system in the education practice activities related to "protecting minority culture" and "developing the capability of students in remote rural areas". The following is a detailed description of these two EE practices activities.

As mentioned earlier, one of the obstacles to the EE of ethnic minorities in China at this stage is the over-emphasis of the Han culture in education. According to the capability approach, an important way to realize the EE of ethnic minorities is to focus on their own cultural needs. Only by taking their cultural characteristics and educational experience into consideration can they have the right and freedom to choose a suitable educational model. Therefore, in July 2022, the Chen Yidan Foundation, the main investor of the LFCF project, collaborated with Starry Night Chinese Multicultural, a youth team dedicated to protecting, inheriting and innovating multi-ethnic cultural heritage. Together, they launched a five-day project-based learning camp on Haqniq culture inheritance and innovation in the Meng Song Primary School, Mengsong Village, Menglong Town, Jinghong City, Xishuangbanna

Dai Autonomous Prefecture, Yunnan Province. The detailed itinerary of the summer camp is as follows:

**Table 6.** The syllabus of the camp.

In addition to the development of EE for ethnic minorities, the capability lists and CMYK practice system are also systematically applied to disadvantaged groups in rural and remote areas and further optimized after a series of educational practices. In February 2023, the Chen Yidan Foundation cooperated with the Sustainable Education Innovation Alliance to launch the project of "promoting the development of literacy education and the realization of equitable education in rural areas". In chronological order, the operation mechanism of this project in the coming year (2023.2–2023.12) is presented (see Figure 4).

**Figure 4.** The action of "promoting the development of literacy education and the realization of equitable education in rural areas".

So far, more than 80 volunteer teaching teams have signed up to participate in this initiative, many of which aim to improve the education of disadvantaged groups. A sign language volunteer teaching team from the Xuzhou Qiyan Public Service Center conducted a sign language course on "How to make deaf students in special schools and students in general schools equally and jointly inherit the Yi cultural heritage?". Another team from Shenzhen University conducted the project "How to guide left-behind children to explore the environment and nature in their neighborhoods under the concept of sustainable development?". Although this action is still in the early stage at present, and its effectiveness needs further observation, we hope that this year-long educational action will help literacy capability education to take root, sprout and grow better in rural areas.

Based on actionable research approach, the LFCF project and its existing educational experiments bring together stakeholders to have an equitable dialogue and exchange and develop a series of educational actions to promote the development of human capability. Although officially China has multiple approaches in place to expand university enrollment and increase educational opportunities in rural areas, using engaged scholarship partnership can accelerate this process, because social justice is a commonly shared pursuit [25] (p. 60). The LFCF team's three-year action and its initial results show that engaged scholarships are of great value for and significance to the development of EE. At the same time, it also shows that human-capability-based action research about EE and social justice also needs to apply "engaged scholarship" in order to build a knowledge system that eliminates the deeply rooted and increasingly widening structural, institutional, social and cultural inequalities. As St. John and others pointed out in 2018, it is still a lofty mission of the academic community to participate in academic research that supports social actions and efforts to reduce various inequities in human society [29] (p. 51). It should be noted that the focus of the engaged scholarship is not on critique but on construction, which is also the core of actionable research. Educators should not only describe or criticize the current situation of the world, but rather think about how our actions today could create a future better educational world.

## **5. Conclusions**

We advocate the combined use of the concept of capability and actionable research to organize multi-stakeholder conversations in order to deal with the problem of EI. The way we look at education is challenged by a paradigm shift from an economic orientation to human development, with human beings placed at the center of education and human capability development. A human-centered perspective is premised on expanding how people can choose to live freely, rather than promoting socio-economic development. Although some progress has been made in the development of educational equity in China at this stage, there are differences in the ability of individuals to convert resources into functions, and the government's emphasis on a balanced allocation of resources at the macro level cannot actually solve the problem of educational equity completely. From the Human Capability Perspective, we will constantly ask whether education enables individuals the "capability" to freely choose their lifestyles. We will explore possible

solutions and work together to promote equity in education. In doing so, challenges arising from the gap between theory and practice are inevitable. However, more dimensions of educational equity and more possibilities for improving educational inequalities will be demonstrated. It can be said that the LFCF project and the educational experiments that we have undertaken are a prototype of such possibilities.

Investigating and solving the EI problem from the perspective of human capability needs more voices from scholars, policymakers, social groups, and disadvantaged groups. Based on the theory of human capability, the reform of educational equity is no longer a matter of filling in or repairing, nor is it a matter of fine-tuning data measurement or stratification structures, but an innovation of individual educational development based on human capability and a holistic educational turn needs more joint actions of all social groups.

**Author Contributions:** Conceptualization, M.L. (Mingmei Li), M.L. (Min Liu) and C.W.; methodology, M.L. (Mingmei Li); figures, M.L. (Mingmei Li) and M.L. (Min Liu); writing—original draft preparation, M.L. (Mingmei Li), H.W., M.L. (Min Liu) and X.H.; writing—review and editing, M.L. (Mingmei Li) and M.L. (Min Liu); supervision, C.W.; funding acquisition, C.W. All authors have read and agreed to the published version of the manuscript.

**Funding:** This paper is funded by the International Joint Research Project "Education Development and Social Justice", Faculty of Education, Beijing Normal University (ICER201908).

**Institutional Review Board Statement:** Ethical review and approval were waived for this study as we did not collect any information that could identify the participants during the data collection process. Therefore, according to the ethical review regulations, such research does not require ethical review.

**Informed Consent Statement:** Written informed consent has been obtained from the subjects involved in the study.

**Data Availability Statement:** The data that support the findings of this study will be made available from the authors upon reasonable request.

**Acknowledgments:** The authors would like to thank Chen Yidan Foundation for supporting the actionable case.

**Conflicts of Interest:** The authors declare no conflict of interest.

### **References**

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*3.6. In Vitro α-Glucosidase Inhibitory Assay*

The assay of α-glucosidase inhibitory activity of compounds was adopted from [63]. Briefly, 100 μL of DMSO and 100 μL of α-glucosidase enzyme (5 U/mL in 0.15 M HEPES buffer) were added to 100 μL substrate (0.1 M sucrose solution dissolved into 0.15 M HEPES buffer). The mixture was vortexed for 5 sec and then incubated at 37 ◦C for 30 min to allow for enzymatic reaction. After incubation, the reaction was stopped by heating at 100 ◦C for 10 min in a block incubator. The formation of glucose was determined by means of glucose oxidase method, using a BF-5S Biosensor (Oji Scientific Instruments, Hyogo, Japan). Mathematically, α-glucosidase inhibitory activity of each sample was calculated according to this equation: (Average value of control (Ac) − average value of the sample (As))/Ac × 100.

The IC50 values were calculated from plots of log concentration of inhibitor concentration against the percentage inhibition curves, using Microsoft Excel 2016. The data were expressed as mean ± standard deviation (SD) of at least three independent experiments (*n* = 3).

#### **4. Conclusions**

The global quest for anti-obesity as well as anti-diabetic drugs is currently ongoing, as obesity and its complications continue to afflict the world's population, warranting the discovery of new therapeutic regimens. A high-fat diet induced obesity model in rats was used for the assessment of anti-obese activity of *A. carambola* leaf extract, in relation to its phenolic composition. To the best of our knowledge, this study presents the first comprehensive attempt to reveal the in vivo anti-obese activity of *A. carambola* leaf extract, leading to the isolation of new bioactive components. Oral administration of *A. carambola* leaf extract enhanced all obesity complications, viz., dyslipidemia, hyperglycemia, insulin resistance, and oxidative stress, and exhibited significant anti-obesity activity in obese rats (Figure 5). Further, the effect of CLL was significantly better than Orly in almost all tested biochemical parameters, except for elevated uric acid level, although Orly revealed better reduction in body weight gain.

Multiple chromatographic approaches of the leaf extract led to the isolation of 14 compounds, including 4 flavone glycosides (**1**–**4**) and 10 dihydrochalcone glycosides (**5**–**12**) with two non-separable mixtures, including four newly described compounds, i.e., **1**, **8**, **11a**, and **11b** were reported for the first time in the literature. Further, in vitro α-glucosidase inhibitory activity assessment of isolated compounds revealed the strong potency of isolated flavone glycosides, viz., compounds **1**, **2**, **3**, and **4**, as α-glucosidase inhibitors, compared to dihydrochalcone glycosides, except for compound **12**. These results suggest for the role of flavone glycosides in alleviation of the major obesity comorbidity, i.e., diabetes via α-glucosidase inhibition, and has yet to be confirmed for other action mechanisms. An extended approach utilizing detailed studies on the molecular mechanisms of *A. carambola* leaf effect should now follow, together with subclinical and clinical trials on leaf crude extract, to be more conclusive, especially considering the known negative impact of its fruit on kidney functions. Moreover, assessment of the isolated phytoconstituents for their anti-obese activity using in vivo model or targeting other enzymes, i.e., lipases, etc., should now follow to correlate for the extract's potential anti-obesity effect. This study poses *A. carambola* leaf as a new anti-obesity functional food and adds to its effects aside from its fruit's more explored uses.

**Figure 5.** Collective scheme for extraction, isolation, and biological activities performed on *A. carambola* leaves.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/ 10.3390/molecules27165159/s1. HRESIMS: 1H NMR: 13C NMR, 1H-1H COSY, HSQC, and HMBC spectra of compound **1** (Figures S1–S6); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **2** (Figures S7–S11); HRESIMS, 1H NMR, and 13C NMR spectra of compound **3** (Figures S12–S14); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **4** (Figures S15–S19); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **5** (Figures S20–S24); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **6** (Figures S25–S29); HRESIMS, 1H NMR, 13C NMR, DEPT-135, 1H-1H COSY, HSQC, and HMBC spectra of compound **7** (Figures S30–S36); HRESIMS, 1H NMR, 13C NMR, 1H-1H COSY, DEPT-135, HSQC, and HMBC spectra of compound **8** (Figures S37–S43); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **9** (Figures S44–S48); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **10** (Figures S49–S54); HRESIMS, 1H NMR, 13C NMR, HSQC, and HMBC spectra of compound **11** (Figures S55–S60); HRESIMS, 1H NMR, 13C NMR, HSQC, 1H-1H COSY, DEPT-135, and HMBC spectra of compound **12** (Figures S61–S67); Scheme of isolation (Figure S69); UV spectra of isolated compounds (Figure S70); IR spectra of new compounds **1**, **8**, **11** (Figure S71).

**Author Contributions:** Conceptualization, N.S.R., N.H.E.-S., S.A.E.-T. and M.A.F.; data curation, N.S.R. and M.A.F.; formal analysis, N.S.R.; funding acquisition, N.S.R., K.S., M.A.F. and T.E.; investigation, N.S.R., D.A.M., M.S.M., M.A.F. and T.E.; methodology, N.S.R., K.S., S.A.E.-T. and M.A.F.; project administration, N.S.R., K.S., N.H.E.-S., S.A.E.-T. and M.A.F.; resources, N.S.R. and K.S.; software, N.S.R. and K.S.; supervision, K.S., N.H.E.-S., S.A.E.-T., D.A.M., Z.A.A. and M.A.F.; validation, N.S.R., D.A.M. and M.A.F.; visualization, N.S.R., M.S.M. and M.A.F.; writing—original draft, N.S.R.; writing—review and editing, N.S.R., K.S., N.H.E.-S., S.A.E.-T., M.S.M., Z.A.A., M.A.F. and T.E. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by a fellowship supported by the Egyptian Cultural Affairs and Missions sector, Ministry of Higher Education, Egypt. The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover.

**Institutional Review Board Statement:** All animal experiments were carried out according to the research protocols established by Research Ethics Committee in Faculty of Pharmacy, Cairo University, and by Medical Research Ethics Committee (MREC) in NRC, which follow the recommendations of the National Institutes of Health Guide for the Care and Use of Laboratory Animals Ethical Approval Certificate No. MP (1959).

**Acknowledgments:** The Egyptian Cultural Affairs and Missions sector, Ministry of Higher Education, Egypt, is acknowledged for the fellowship and financial support offered to N.S.R.

**Conflicts of Interest:** The authors declare no conflict of interest.