the invention is directed to methods of modulating ischemic injury in tissues and organs , including donor tissue and organs and intact tissue and organs . the invention is further directed to methods of increasing time to ischemic injury in such tissues and organs . the invention is further directed to storing and preserving donor tissues and organs . such methods utilize compositions comprising amnion - derived cellular cytokine solution . the accs compositions may be formulated for sustained - release , targeted - release , timed - release , extended - release , etc . and may be used alone or in combination with various suitable active agents .