the present invention discloses a method for assembling a 3d microelectrode structure . firstly , 2d microelectrode arrays are stacked to form a 3d microelectrode array via an auxiliary tool . then , the 3d microelectrode array is assembled to a carrier chip to form a 3d microelectrode structure . the present invention uses an identical auxiliary tool to assemble various types of 2d microelectrode arrays having different shapes of probes to the same carrier chip . therefore , the method of the present invention increases the design flexibility of probes . the present invention also discloses a 3d microelectrode structure , which is fabricated according to the method of the present invention and used to perform 3d measurement of biological tissues .