the present invention relates to a novel method for the preparation of a pooled or mixed population of cryopreserved cells . in particular , the invention entails the rapid thaw of cells , donated from a single individual , which are mixed to create a heterogeneous population and then cryopreserved . the invention also concerns preparations of multi - cryopreserved cells to increase viability prior to immediate use . the process entails reducing exposure to chemical and physical stresses to increase the resultant number of viable cells .