provided are methods , kits , and systems useful in the performance of analysis and reporting of highly polymorphic loci , including , in particular , the performance of analysis and reporting of hla typing . combining one - step sequencing and sequence - specific oligonucleotide probe hybridization , the methods , kits , and systems offer improved efficiency of hla typing while providing detailed sequencing information . in certain embodiments the methods and systems comprise one or more software applications to facilitate data acquisition , processing , and reporting .