a recombinant adenovirus target - oriented co - expressing human p53 variant and p53aip1 is disclosed , which is inserted an expression cassette co - expressing human p53 variant and p53aip1 in e1 deletion region of the adenovirus . the expression cassette consists of tumor specific promoter , human p53 variant , internal ribosome entry site , human p53aip1 , and sv40 .