the invention provides method of fabricating a scaffold comprising a fluidic network , including the steps of : generating an initial vascular layer for enclosing the chamber and providing fluid to the cells , the initial vascular layer having a network of channels for fluid ; translating the initial vascular layer into a model for fluid dynamics analysis ; analyzing the initial vascular layer based on desired parameters selected from the group consisting of a characteristic of a specific fluid , an input pressure , an output pressure , an overall flow rate and combinations thereof to determine sheer stress and velocity within the network of channels ; measuring the sheer stress and the velocity and comparing the obtained values to predetermined values ; determining if either of the shear stress or the velocity are greater than or less than the predetermined values , and optionally modifying the initial vascular layer and repeating steps -. the invention also provides compositions comprising a vascular layer for use in tissue lamina as well as a medical devices having a vascular layer and kits .