this invention relates to the separation of complex protein mixtures by lowering or by raising their electrolytic concentration by electrodialysis followed by chilling , ph adjustment , filtration , and / or centrifugation and optionally thereafter restoration of the lost electrolyte or removal of the gained electrolyte and water . this technique is especially adaptable for therapeutic plasma exchange performed in situ for immunepheresis i . e . removal of globulin or immunecomplexes implicated in autoimmune diseases . the techniques are also applicable for antihemophilic factor separation and purification , and separation of proteins from whey .