the present invention provides recombinant dna molecules comprising a sequence encoding a pseudorabies virus glycoprotein selected from the group consisting of gi , gp50 , and gp63 , host cells transformed by said recombinant dna molecule sequences , the gi , gp50 and gp63 polypeptides . the present invention also provides subunit vaccines for prv , methods for protecting animals against prv infection and methods for distinguishing between infected and vaccinated animals .