in accordance with the present invention there may be employed conventional molecular biology , microbiology , and recombinant dna techniques within the skill of the art . such techniques are explained fully in the literature . see , e . g ., sambrook et al , “ molecular cloning : a laboratory manual ” ( 1989 ); “ current protocols in molecular biology ” volumes i - iii [ ausubel , r . m ., ed . ( 1994 )]; “ cell biology : a laboratory handbook ” volumes - 1 - iii [ j . e . celis , ed . ( 1994 ))]; “ current protocols in immunology ” volumes i - iii [ coligan , j . e ., ed . ( 1994 )]; “ oligonucleotide synthesis ” ( m . j . gait ed . 1984 ); “ nucleic acid hybridization ” [ b . d . hames & amp ; s . j . higgins eds . ( 1985 )]; “ transcription and translation ” [ b . d . hames & amp ; s . j . higgins , eds . ( 1984 )]; “ animal cell culture ” [ r . i . freshney , ed . ( 1986 )]; “ immobilized cells and enzymes ” [ irl press , ( 1986 )]; b . perbal , “ a practical guide to molecular cloning ” ( 1984 ). if appearing herein , the following terms shall have the definitions set out below . the terms active fragments , structural analogs , functional analogs or mimics , as used in conjunction with the term trip , and any variants not specifically listed , may be used herein interchangeably , and as used throughout the present application and claims refer to proteinaceous material including single or multiple proteins , and extends to those proteins having the amino acid sequence data described herein and presented in fig2 a , seq id no : 1 , seq id no : 2 , seq id no : 3 and seq id no : 4 , and fig2 b , seq id no : 5 and seq id no : 6 , and the profile of activities set forth herein and in the claims . accordingly , proteins displaying substantially equivalent or altered activity are like - wise contemplated . these modifications may be deliberate , for example , such as modifications obtained through site - directed mutagenesis , or may be accidental , such as those obtained through mutations in hosts that are producers of the complex or its named subunits . also , the term trip is intended to include within its scope proteins specifically recited herein as well as all homologous analogs and allelic variations . the terms agonists or antagonists when used in conjunction with the term trip , and in addition to their ordinary meanings to one skilled in the art , refer to proteinaceous or non - proteinaceous materials , including inorganic or organic combinations of elements , compounds , compositions or peptidomimetics which function positively or negatively , inhibit , up - or down - regulate functions similar to that produced by the amino acid sequence or fragments thereof as described herein and presented in fig2 a , seq id no : 1 , seq id no : 2 , seq id no : 3 and seq id no : 4 , and fig2 b , seq id no : 5 and seq id no : 6 , and the profile of activities set forth herein and in the claims . a dna sequence is “ operatively linked ” to an expression control sequence when the expression control sequence controls and regulates the transcription and translation of that dna sequence . the term “ operatively linked ” includes having an appropriate start signal ( e . g ., atg ) in front of the dna sequence to be expressed and maintaining the correct reading frame to permit expression of the dna sequence under the control of the expression control sequence and production of the desired product encoded by the dna sequence . if a gene that one desires to insert into a recombinant dna molecule does not contain an appropriate start signal , such a start signal can be inserted in front of the gene . the term “ standard hybridization conditions ” refers to salt and temperature conditions substantially equivalent to 5 × ssc and 65 ° c . for both hybridization and wash . however , one skilled in the art will appreciate that such “ standard hybridization conditions ” are dependent on particular conditions including the concentration of sodium and magnesium in the buffer , nucleotide sequence length and concentration , percent mismatch , percent formamide , and the like . also important in the determination of “ standard hybridization conditions ” is whether the two sequences hybridizing are rna - rna , dna - dna or rna - dna . such standard hybridization conditions are easily determined by one skilled in the art according to well known formulae , wherein hybridization is typically 10 - 20 ° c . below the predicted or determined t m with washes of higher stringency , if desired . the amino acid residues described herein are preferred to be in the “ l ” isomeric form . however , residues in the “ d ” isomeric form can be substituted for any l - amino acid residue as long as the desired fuctional properties is retained by the polypeptide . in keeping with standard polypeptide nomenclature , abbreviations for amino acid residues are shown in the following table of correspondence : it should be noted that all amino - acid residue sequences are represented herein by formulae whose left and right orientation is in the conventional direction of amino - terminus to carboxy - terminus . the above table is presented to correlate the three - letter and one - letter notations which may appear alternately herein and especially in the figures . the phrase “ pharmaceutically acceptable ” refers to molecular entities and compositions that are physiologically tolerable and which do not typically produce an allergic or similar undesired hyper / hypo - reaction when administered to a human . the phrase “ therapeutically effective amount ” is used herein to mean an amount sufficient to prevent , and prefer - ably reduce by at least about 30 percent , more preferably by at least 50 percent , most preferably by at least 90 percent , a clinically significant change in the s phase activity of a target cellular mass , or other feature of pathology such as for example , abnormal lymphocyte counts , morphology , differentiation or distribution . as stated above , the present invention also relates to a recombinant dna molecule or cloned gene , or a degenerate variant thereof , which encodes the full length sequences shown in fig2 a ( seq id no : 1 ) ( seq id no : 2 ), or a fragment thereof ; preferably a nucleic acid molecule , in particular a recombinant dna molecule or cloned gene , encoding the nucleotide sequences shown in fig8 ( seq id no : 7 ) ( seq id no : 8 ). the possibilities both diagnostic and therapeutic that are raised by the existence of the trip , derive from the fact that the factors appear to participate in direct and causal protein — protein interaction between the tnfr2 or cd30 receptors via interaction with traf proteins , specifically the traf2 homo - oligomer . as suggested earlier and elaborated further on herein , the present invention contemplates pharmaceutical intervention in the cascade of reactions in which the trip is implicated , to modulate the activity initiated by the induction of nf - kb activation by tnfr2 , cd30 , tnfr1 and tradd specifically through the traf2 - mediated pathway . thus , in instances where it is desired to reduce or inhibit the induction of nf - kb activation resulting from a particular stimulus or factor , an appropriate inhibitor of trip could be introduced to block the traf2 - mediated pathway . correspondingly , in situations where insufficient trip activity is taking place , the remedy could be through the introduction of additional quantities of potentiators of trip expression , potentiators of expression of trip analogs , fragments and the like , or chemicals or pharmaceutical cognates which enhance trip expression or function equivalently to trip . as discussed earlier , binding partners to trip or proteins as represented by seq id nos : 1 - 6 , or other ligands or agents exhibiting either mimicry or antagonism to the trip activity or control over their production , may be prepared with a suitable carrier and at a strength effective for administration by various means to a patient experiencing an adverse medical condition associated with specific deficiency or excess in trip or its activity for the treatment thereof . a variety of administrative techniques may be utilized , among them parenteral techniques such as subcutaneous , intravenous and intraperitoneal injections , catheterizations and the like . average quantities of trip or their subunits may vary and in particular should be based upon the recommendations and prescription of a qualified physician or veterinarian . also , antibodies including both polyclonal and mono - clonal antibodies , and drugs that modulate the production or activity of trip and / or their subunits may possess certain diagnostic applications and may for example , be utilized for the purpose of detecting and / or measuring conditions which demonstrate abnormal trip activity . for example , trip or its subunits may be used to produce both polyclonal and monoclonal antibodies to themselves in a variety of cellular media , by known techniques such as the hybridoma technique utilizing , for example , fused mouse spleen lymphocytes and myeloma cells . likewise , small molecules that mimic or antagonize the activity ( ies ) of trip may be discovered or synthesized , and may be used in diagnostic and / or therapeutic protocols . the general methodology for making monoclonal antibodies by hybridomas is well known . immortal , antibody - producing cell lines can also be created by techniques other than fusion , such as direct transformation of b lymphocytes with oncogenic dna , or transfection with epstein - barr virus . see , e . g ., m . schreier et al ., “ hybridoma techniques ” ( 1980 ); hammerling et al ., “ monoclonal antibodies and t - cell hybridomas ” ( 1981 ); kennett et al ., “ monoclonal antibodies ” ( 1980 ); see also u . s . pat . nos . 4 , 341 , 761 ; 4 , 399 , 121 ; 4 , 427 , 783 ; 4 , 444 , 887 ; 4 , 451 , 570 ; 4 , 466 , 917 ; 4 , 472 , 500 ; 4 , 491 , 632 ; 4 , 493 , 890 . panels of monoclonal antibodies produced against trip can be screened for various properties ; i . e ., isotype , epitope , affinity , etc . of particular interest are monoclonal antibodies that neutralize the activity of trip or its subunits . such monoclonals can be readily identified in trip activity assays . preferably , the anti - trip antibody used in the diagnostic methods of this invention is an affinity purified polyclonal antibody . more preferably , the antibody is a monoclonal antibody ( mab ). in addition , it is preferable for the anti - trip antibody molecules used herein be in the form of fab , fab ′, f ( ab ′) 2 or f ( v ) portions of whole antibody molecules . as suggested earlier , the diagnostic method of the present invention comprises examining a cellular sample or medium by means of an assay including an effective amount of an antagonist to trip , such as an anti - trip antibody , preferably an affinity - purified polyclonal antibody , and more preferably a mab . it is preferable for the anti - trip antibody molecules used herein be in the form of fab , fab ′, f ( ab ′) 2 or f ( v ) portions or whole antibody molecules . methods for producing polyclonal anti - polypeptide antibodies are well - known in the art . see for example u . s . pat . no . 4 , 493 , 795 to nestor et al . a monoclonal antibody , typically containing fab and / or f ( ab ′) 2 portions of useful antibody molecules , can be prepared using the hybridoma technology described in antibodies - a laboratory manual , harlow and lane , eds ., cold spring harbor laboratory , new york ( 1988 ), which is incorporated herein by reference . briefly , to form the hybridoma from which the monoclonal antibody composition is produced , a myeloma or other self - perpetuating cell line is fused with lymphocytes obtained from the spleen of a mammal hyperimmunized with trip , a trip - binding portion thereof , or an origin - specific dna - binding portion thereof . splenocytes are typically fused with myeloma cells using polyethylene glycol ( peg ) 6000 . hybridomas producing a mono - clonal antibody useful in practicing this invention are identified by their ability to immunoreact with the present trip and their ability to inhibit specified trip activity in target cells . a monoclonal antibody useful in practicing the present invention can be produced by initiating a monoclonal hybridoma culture comprising a nutrient medium containing a hybridoma that secretes antibody molecules of the appropriate antigen specificity . the culture is maintained under conditions and for a time period sufficient for the hybridoma to secrete the antibody molecules into the medium . the anti - body - containing medium is then collected . the antibody molecules can then be further isolated by well - known techniques . media useful for the preparation of these compositions are both well - known in the art and commercially available and include synthetic culture media , inbred mice and the like . an exemplary synthetic medium is dulbecco &# 39 ; s minimal essential medium ( dmem ; dulbecco et al ., virol . 8 : 396 ( 1959 )) supplemented with 4 . 5 gm / l glucose , 20 mm glutamine , and 20 % fetal calf serum . an exemplary inbred mouse strain is the balb / c . methods for producing monoclonal anti - trip antibodies are also well - known in the art . see niman et al ., proc . natl . acad . sci . usa , 80 : 4949 - 4953 ( 1983 ). typically , the present trip or a protein analog is used either alone or conjugated to an immunogenic carrier . the hybridomas are screened for the ability to produce an antibody that immunoreacts with the trip or protein analog . the present invention further contemplates therapeutic compositions useful in practicing the therapeutic methods of this invention . a subject therapeutic composition includes , in admixture , a pharmaceutically acceptable excipient ( carrier ) and one or more of trip , a protein analog thereof or fragment thereof , as described herein as an active ingredient . in a preferred embodiment , the composition comprises an antigen capable of modulating the specific binding of the present trip within a target cell . the preparation of therapeutic compositions which contain polypeptides , analogs or active fragments as active ingredients is well understood in the art . typically , such compositions are prepared as injectables , either as liquid solutions or suspensions , however , solid forms suitable for solution in , or suspension in , liquid prior to injection can also be prepared . the preparation can also be emulsified . the active therapeutic ingredient is often mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredient . suitable excipients are , for example , water , saline , dextrose , glycerol , ethanol , or the like and combinations thereof . in addition , if desired , the composition can contain minor amounts of auxiliary substances such as wetting or emulsifying agents , ph buffering agents which enhance the effectiveness of the active ingredient . a polypeptide , analog or active fragment can be formulated into the therapeutic composition as neutralized pharmaceutically acceptable salt forms . pharmaceutically acceptable salts include the acid addition salts ( formed with the free amino groups of the polypeptide or antibody molecule ) and which are formed with inorganic acids such as , for example , hydrochloric or phosphoric acids , or such organic acids as acetic , oxalic , tartaric , mandelic , and the like . salts formed from the free carboxyl groups can also be derived from inorganic bases such as , for example , sodium , potassium , ammonium , calcium , or ferric hydroxides , and such organic bases as isopropylamine , trimethylamine , 2 - ethylamino ethanol , histidine , procaine , and the like . the therapeutic compositions are conventionally administered intravenously , for example , by injection of a unit dose . the term “ unit dose ” when used in reference to a therapeutic composition of the present invention refers to physically discrete units suitable as unitary dosage for humans , each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with the required diluent ; i . e ., carrier , or vehicle . the compositions are administered in a manner compatible with the dosage formulation , and in a therapeutically effective amount . the quantity to be administered depends on the subject to be treated , capacity of the subject &# 39 ; s immune system to utilize the active ingredient , and degree of inhibition or neutralization of trip binding capacity desired . precise amounts of active ingredient required to be administered depend on the judgment of the practitioner and are peculiar to each individual . however , suitable dosages may range from about 0 . 1 to 20 , preferably about 0 . 5 to about 10 , and more preferably one to several , milligrams of active ingredient per kilogram body weight of individual per day and depend on the route of administration . suitable regimes for initial administration and booster shots are also variable , but are typified by an initial administration followed by repeated doses at one or more hour intervals by a subsequent injection or other administration . alternatively , continuous intravenous infusion sufficient to maintain concentrations of ten nanomolar to ten micromolar in the blood are contemplated . another feature of this invention is the expression of the dna sequences disclosed herein . as is well known in the art , dna sequences may be expressed by operatively linking them to an expression control sequence in an appropriate expression vector and employing that expression vector to transform an appropriate unicellular host . such operative linking of a dna sequence of this invention to an expression control sequence , of course , includes , if not already part of the dna sequence , the provision of an initiation codon , atg , in the correct reading frame upstream of the dna sequence . a wide variety of host / expression vector combinations may be employed in expressing the dna sequences of this invention . useful expression vectors , for example , may consist of segments of chromosomal , non - chromosomal and synthetic dna sequences . suitable vectors include derivatives of sv40 and known bacterial plasmids , e . g ., e . coli plasmids co1 e1 , pcr1 , pbr322 , pmb9 and their derivatives , plasmids such as rp4 ; phage dnas , e . g ., the numerous phage derivatives , e . g ., nm989 , and other phage dna , e . g ., m13 and filamentous single stranded phage dna ; yeast plasmids such as the 2μ plasmid or derivatives thereof ; vectors useful in eukaryotic cells , such as vectors useful in insect or mammalian cells ; vectors derived from combinations of plasmids and phage dnas , such as plasmids that have been modified to employ phage dna or other expression control sequences ; and the like . any of a wide variety of expression control sequences — sequences that control the expression of a dna sequence operatively linked to it — may be used in these vectors to express the dna sequences of this invention . such useful expression control sequences include , for example , the early or late promoters of sv40 , cmv , vaccinia , polyoma or adenovirus , the lac system , the trp system , the tac system , the trc system , the ltr system , the control regions of fd coat protein , the promoter for 3 - phosphoglycerate kinase or other glycolytic enzymes , the promoters of acid phosphatase ( e . g ., pho5 ), the promoters of the yeast α - mating factors , and other sequences known to control the expression of genes of prokaryotic or eukaryotic cells or their viruses , and various combinations thereof . a wide variety of unicellular host cells are also useful in expressing the dna sequences of this invention . these hosts may include well known eukaryotic and prokaryotic hosts , such as strains of e . coli , pseudomonas , bacillus , streptomyces , fungi such as yeasts , and animal cells , such as cho , r1 . 1 , b - w and l - m cells , african green monkey kidney cells ( e . g ., cos 1 , cos 7 , bsc1 , bsc40 , and bmt10 ), insect cells ( e . g ., sf9 ), and human cells and plant cells in tissue culture . it will be understood that not all vectors , expression control sequences and hosts will function equally well to express the dna sequences of this invention . neither will all hosts function equally well with the same expression system . however , one skilled in the art will be able to select the proper vectors , expression control sequences , and hosts without undue experimentation to accomplish the desired expression without departing from the scope of this invention . for example , in selecting a vector , the host must be considered because the vector must function in it . the vector &# 39 ; s copy number , the ability to control that copy number , and the expression of any other proteins encoded by the vector , such as antibiotic markers , will also be considered . in selecting an expression control sequence , a variety of factors will normally be considered . these include , for example , the relative strength of the system , its controllability , and its compatibility with the particular dna sequence or gene to be expressed , particularly as regards potential secondary structures . suitable unicellular hosts will be selected by consideration of , e . g ., their compatibility with the chosen vector , their secretion characteristics , their ability to fold proteins correctly , and their fermentation requirements , as well as the toxicity to the host of the product encoded by the dna sequences to be expressed , and the ease of purification of the expression products . considering these and other factors a person skilled in the art will be able to construct a variety of vector / expression control sequence / host combinations that will express the dna sequences of this invention on fermentation or in large scale animal culture . it is further intended that trip analogs may be prepared from nucleotide sequences of the protein complex / subunit derived within the scope of the present invention . analogs , such as fragments , may be produced , for example , by pepsin digestion of trip material . other analogs , such as muteins , can be produced by standard site - directed mutagenesis of trip coding sequences . analogs exhibiting “ trip activity ” such as small molecules , whether functioning as promoters or inhibitors , may be identified by known in vivo and / or in vitro assays . as mentioned above , a dna sequence encoding trip can be prepared synthetically rather than cloned . the dna sequence can be designed with the appropriate codons for the trip amino acid sequence . in general , one will select preferred codons for the intended host if the sequence will be used for expression . the complete sequence is assembled from over - lapping oligonucleotides prepared by standard methods and assembled into a complete coding sequence . see , e . g ., edge , nature , 292 : 756 ( 1981 ); nambair et al ., science , 223 : 1299 ( 1984 ); jay et al ., j . biol . chem ., 259 : 6311 ( 1984 ). synthetic dna sequences allow convenient construction of genes which will express trip analogs or “ muteins ”. alternatively , dna encoding muteins can be made by site - directed mutagenesis of native trip genes or cdnas , and muteins can be made directly using conventional polypeptide synthesis . a general method for site - specific incorporation of unnatural amino acids into proteins is described in christopher j . noren , spencer j . anthony - cahill , michael c . griffith , peter g . schultz , science , 244 : 182 - 188 ( april 1989 ). this method may be used to create analogs with unnatural amino acids . the present invention extends to the preparation of antisense oligonucleotides and ribozymes that may be used to interfere with the expression of the trip at the translational level . this approach utilizes antisense nucleic acid and ribozymes to block translation of a specific mrna , either by masking that mrna with an antisense nucleic acid or cleaving it with a ribozyme . antisense nucleic acids are dna or rna molecules that are complementary to at least a portion of a specific mrna molecule . ( see weintraub , 1990 ; marcus - sekura , 1988 .) in the cell , they hybridize to that mrna , forming a double stranded molecule . the cell does not translate an mrna in this double - stranded form . therefore , antisense nucleic acids interfere with the expression of mrna into protein . oligomers of about fifteen nucleotides and molecules that hybridize to the aug initiation codon will be particularly efficient , since they are easy to synthesize and are likely to pose fewer problems than larger molecules when introducing them into trip - producing cells . antisense methods have been used to inhibit the expression of many genes in vitro ( marcus - sekura , 1988 ; hambor et al ., 1988 ). ribozymes are rna molecules possessing the ability to specifically cleave other single stranded rna molecules in a manner somewhat analogous to dna restriction endonucleases . ribozymes were discovered from the observation that certain mrnas have the ability to excise their own introns . by modifying the nucleotide sequence of these rnas , molecules can be engineered so that they recognize specific nucleotide sequences in an rna molecule and cleave it . because they are sequence - specific , only mrnas with particular sequences are inactivated . investigators have identified two types of ribozymes , tetrahymena - type and “ hammerhead ”- type . ( hasselhoff and gerlach , 1988 ) tetrahymena - type ribozymes recognize four - base sequences , while “ hammerhead ”- type recognize eleven - to eighteen - base sequences . the longer the recognition sequence , the more likely it is to occur exclusively in the target mrna species . therefore , hammerhead - type ribozymes are preferable to tetrahymena - type ribozymes for inactivating a specific mrna species , and eighteen base recognition sequences are preferable to shorter recognition sequences . the dna sequences described herein may thus be used to prepare antisense molecules against , and ribozymes that cleave mrnas for trip and their ligands . the present invention also relates to a variety of diagnostic applications , including methods for detecting the presence of stimuli such as the earlier referenced poly - peptide ligands , by reference to their ability to elicit the activities which are mediated by the present trip . as stated earlier , trip can be used to produce antibodies to itself by a variety of known techniques , and such antibodies could then be isolated and utilized as in tests for the presence of particular trip activity in suspect target cells . as described in detail above , antibody ( ies ) to trip can be produced and isolated by standard methods including the well known hybridoma techniques . for convenience , the anti - bodies to htrip will be referred to herein as ab 1 and anti - bodies raised in another species as ab 2 . the presence of trip in cells can be ascertained by the usual immunological procedures applicable to such determinations . a number of useful procedures are known . three such procedures which are especially useful utilize antibody ab 1 labeled with a detectable label , antibody ab 2 labeled with a detectable label , or a combination of antibodies ab 1 and ab 2 each labeled with a detectable label . the procedures may be summarized by the following equations wherein the asterisk indicates that the particle is labeled , wherein , for example , ab 1 is anti - htrip and ab 2 is anti - trip of a non - human specie : a . trip + ab 1 *= tripab 1 * b . trip + ab 2 * = tripab 2 * c . trip + ab 1 + ab 2 * = tripab 1 ab 2 * the procedures and their application are all familiar to those skilled in the art and accordingly may be utilized within the scope of the present invention . the “ competitive ” procedure , procedure a , is described in u . s . pat . nos . 3 , 654 , 090 and 3 , 850 , 752 . procedure c , the “ sandwich ” procedure , is described in u . s . pat . nos . re 31 , 006 and 4 , 016 , 043 . still other procedures are known such as the “ double antibody ,” or “ dasp ” procedure . in each instance , trip forms complexes with one or more antibody ( ies ) or binding partners and one member of the complex is labeled with a detectable label . the fact that a complex has formed and , if desired , the amount thereof , can be determined by known methods applicable to the detection of labels . it will be seen from the above , that a characteristic property of ab 2 is that it will react with ab 1 . this is because ab 1 raised in one mammalian species has been used in another mammalian species as an antigen to raise the antibody ab 2 . for purposes of this description and claims , ab 1 will be referred to as a primary or anti - htrip antibody , and ab 2 will be referred to as a secondary or anti - ab 1 antibody which can include anti - mtrip . the labels most commonly employed for these studies are radioactive elements , enzymes , chemicals which fluoresce when exposed to ultraviolet light , and others . a number of fluorescent materials are known and can be utilized as labels . these include , for example , fluorescein , rhodamine , auramine , texas red , amca blue and lucifer yellow . a particular detecting material is anti - rabbit antibody prepared in goats and conjugated with fluorescein through an isothiocyanate . binding partner ( s ) for trip can also be labeled with a radioactive element or with an enzyme . the radioactive label can be detected by any of the currently available counting procedures . the preferred isotope may be selected from 3 h , 14 c , 32 p , 35 s , 36 cl , 51 cr , 57 co , 58 co 59 fe 90 y , 125 i , 131 i , and 186 r . enzyme labels are likewise useful , and can be detected by any of the presently utilized calorimetric , spectrophotometric , fluorospectrophotometric , amperometric or gasometric techniques . the enzyme is conjugated to the selected particle by reaction with bridging molecules such as carbodiimides , diisocyanates , glutaraldehyde and the like . many enzymes which can be used in these procedures are known and can be utilized . the preferred are peroxidase , β - glucuronidase , β - d - glucosidase , β - d - galactosidase , urease , glucose oxidase plus peroxidase and alkaline phosphatase . u . s . pat . nos . 3 , 654 , 090 ; 3 , 850 , 752 ; and 4 , 016 , 043 are referred to by way of example for their disclosure of alternate labeling material and methods . in a further embodiment of this invention , commercial test kits suitable for use by a medical specialist may be prepared to determine the presence or absence of predetermined trip activity or predetermined trip activity - capability in suspected target cells . in accordance with the testing techniques discussed above , one class of such kits will contain at least one labeled anti - trip antibody or another suitable binding partner , for instance an antibody specific to fragments of trip , and directions for use depending upon the method selected , e . g ., “ competitive ,” “ sandwich ,” “ dasp ” and the like . the kits may also contain peripheral reagents such as buffers , stabilizers and the like for enhancing storage and durable use . accordingly , a test kit may be prepared for the demonstration of the presence or capability of cells for predetermined trip activity , comprising : ( a ) a predetermined amount of at least one immuno - chemically reactive component obtained by direct or indirect contact with trip or any fragments or subunits of a full sequence selected from those shown in fig2 a ( seq id no : 1 ) ( seq id no : 2 ) or a binding partner thereto , and attaching a detectable label to the component ; ( b ) other reagents ; and ( c ) directions for use of said kit . in a further variation , the test kit may be prepared and used for the purposes stated above , which operates according to a predetermined protocol ( e . g . “ competitive ,” “ sandwich ,” “ double antibody ,” etc . ), and comprises : ( a ) a labeled component which has been obtained by coupling a anti - trip antibody to a detectable label ; ( b ) one or more additional immunochemical reagents of which at least one reagent is a ligand or an immobilized ligand , which ligand is selected from the group consisting of : ( i ) a ligand capable of binding with the labeled component ( a ); ( ii ) a ligand capable of binding with a binding partner of the labeled component ( a ); ( iii ) a ligand capable of binding with at least one of the component ( s ) to be determined ; and ( iv ) a ligand capable of binding with at least one of the binding partners of at least one of the component ( s ) to be determined ; and ( c ) directions for the performance of a protocol for the detection and / or determination of one or more components of an immunochemical reaction between trip and anti - trip antibody and the various specific ligand interactions . the following examples are presented in order to more fully illustrate the preferred embodiments of the invention . they should in no way be construed , however , as limiting the broad scope of the invention . in this example the yeast two - hybrid system was used to isolate trip taking advantage of its role as a traf1 - interacting protein . by screening cdna libraries derived from mouse thymocytes , multiple cdna clones representing several distinct proteins were isolated . among these , was one set of cdna - encoded proteins which interacted with both traf1 and traf2 in the two - hybrid assay which was conducted as follows . a bait plasmid peg202 - traf1 [ lee et al ., j . exp . med . 183 : 669 - 674 ( 1996 )] which encodes the lexa - dna binding domain fused to traf1 , was used for a yeast two - hybrid screening of a mouse thymocyte cdna library . the isolation of positive clones and subsequent analyses were carried out as described in lee et al ., j . exp . med ., id . the interaction of proteins in the two - hybrid assay was scored by the b - galactosidase activity of yeast transformants containing both activators and baits upon galactose induction as described in lee et al ., proc . natl . acad . sci . usa , 93 : 9699 - 9703 ( 1996 ). in brief , yeast cells were permeabilized with 0 . 0025 % sds and 5 % chloroform , and the cell debris was removed by centrifugation . the b - galactosidase assay was performed at 25 ° c . and od420 was measured . the trip cdna insert of approximately 1 . 0 kb isolated by two - hybrid screening was used as a probe to screen mouse thymocytes and t cell hybridoma cdna libraries in lambda - zap ( strategene ) as described in park et al ., immunity , 4 : 583 - 591 ( 1996 ). a human thymocyte cdna library in lambda - gt10 ( clontech ) was similarly screened using full - length mtrip cdna . for sequence analysis of mtrip and htrip , several overlapping cdna clones were sequenced using the sequenase kit ( united states biochemical co ., cleveland , ohio ). northern analysis of mouse tissue rna was performed as described in park et al ., immunity , id . analysis of the dna sequence of the traf1 - and traf2 - interacting cdna clones revealed that they are derived from a single novel gene named herein as trip . since trip interacted strongly with both traf1 and traf2 in the two hybrid - assay , these proteins were tested for interaction in mammalian cells in the next example . in this example , expression vectors encoding traf1 or traf2 were coexpressed in 293 cells in the presence of an expression vector encoding either gst alone or gst - trip fusion protein . rabbit polyclonal antisera recognizing mtrip were prepared by animal pharm services , inc . using bacteria - produced gst - trip fusion proteins . polyclonal antisera were negatively selected with purified gst proteins before use . anti - traf1 and anti - traf2 abs were from santa cruz biotechnology . the monoclonal ab against the ha epitope ( 12ca5 ) was from babco . recombinant human tnf and il - 1 were from r & amp ; d systems . eukaryotic expression vectors for wild type or mutant forms of traf1 and traf2 , cd8 - tnfr2 , cd8 - cd30 , gst - tnfr2 , and gst - cd30 are described in lee et al ., j . exp . med ., id . and proc . natl . acad . sci . usa , id . expression vectors for tradd was made by cloning the pcr - amplified murine tradd cdna into phbapr - 1 - neo . to generate eukaryotic expression vectors for gst - wild type trip or mutant trips , various trip cdnas were generated by pcr and in - frame cloned into 5 ′- bamhi - noti - 3 ′- sites in pebg vector as described in lee et al ., j . exp . med ., id . transfection of 293 cells were performed in 6 cm dishes by calcium phosphate precipitation as described in lee et al ., j . exp . med ., id . each transfection maintained an equal amount of total dna by adding appropriate amount of the control vector , pcdna3 . 1 ( invitrogen ). forty - eight hours after transfection , luciferase activity was determined and normalized relative to b - galactosidase activity . cell lysates were precipitated with glutathione - sepha - rose beads , and analyzed by western blot analysis with anti - traf1 or anti - traf2 antibodies . the 293 cells were transfected with various combinations of expression vectors and thirty - six hours after transfection , cells were harvested in phosphate - buffered saline / 1 mm phenylmethylsulfonyl fluoride , pelleted , and lysed in lysis buffer ( 20 mm hepes ( ph 7 . 9 ), 100 mm kcl , 300 mm nacl , 10 mm edta , 0 . 1 % np - 40 , plus protease inhibitors ). after lysis , aliquots of cell lysates were incubated with glutathione - sepharose ( pharmacia ) for 2 hr at 4 ° c . the beads were then washed 5 times with the lysis buffer , followed by an additional wash with the lysis buffer lacking np - 40 . the proteins were then recovered by boiling in sds - page sample buffer . the eluted proteins were separated on 10 % sds - page and transferred to immobilon p ( millipore ). the blot was subjected to western analysis using enhanced chemoluminoscence ( ecl ) system ( amersham ). consistent with the yeast two - hybrid assay , gst - trip coprecipitated both traf1 and traf2 , demonstrating that trip can interact directly with traf1 and traf2 in human cells . fig1 depicts the results of western blot analysis . cell lysates before precipitation with glutathione beads were analyzed by western analysis to show that similar amounts of traf1 or traf2 are present in each sample . proteins coprecipitated with gst - fusion proteins were analyzed . traf1 or traf2 are indicated with arrows . full - length sequence of trip was derived from sequence analysis of multiple cdna clones from both thymocyte and t cell cdna libraries . fig2 a shows the full length mouse sequence ( seq id no : 2 ), and the human sequence ( seq id no : is shown with one less amino acid , indicated with a dot at position 302 . dashes indicate positions in the human sequence which are identical to those in the mouse . cysteine and histidine residues defining the ring finger motif are marked by boxes . brackets indicate the coiled - coil regions of trip with the entire domain comprising residues 56 - 275 for both human ( seq id no : 3 ) and mouse ( seq id no : 4 ) trip . within the brackets , amino acids that form the coiled - coil structures are marked by overlying dots , and those that form leucine - zipper structures are indicated in bold . trip mrna is predicted to encode proteins of 470 amino acids . human trip encodes a 469 a . a . protein that is overall 76 % identical to murine trip as shown in fig2 a . the amino acid sequence identity between the n - terminal half of mtrip and htrip ( residues 1 - 270 ) is even higher ( 87 % identical ). a homology search of the trip amino acid sequence revealed that trip is a novel protein with an n - terminal ring finger sequence motif . fig2 b shows a comparison of amino acid sequences from various proteins that contain ring finger motifs . the ring finger domains of htrip ( seq id no : 5 ), mtrip ( seq id no : 6 ), mtraf2 ( seq id no : 9 ), mtraf3 ( seq id no : 10 ), mc - iap1 ( seq id no : 11 ), human proto - oncogene c - cb1 ( seq id no : 12 ), human ring1 ( seq id no : 13 ), human ribonucleoprotein ss - a / ro ( seq id no : 14 ), chicken c - rzf ( seq id no : 15 ), and drosophila neuralized gene , neu ( seq id no : 16 ) are all shown with residues corresponding to the consensus sequence indicated in bold . the n - terminal ring finger motif of trip is followed by an extended coiled - coil domain ( seq id no : 3 ) ( seq id no : 4 ). the coiled - coil domain of trip can be further divided into the n - terminal coiled - coil structure similar to the rod - like tails of myosin heavy chains ( residues 56 - 150 ) [ mclach - lan et al ., nature , 299 : 226 - 231 ( 1982 )], and the c - terminal leucine zipper - like coils ( residues 221 - 260 ) [ landschulz et al ., science , 240 : 1759 - 1764 ( 1988 )], both of which are implicated in protein — protein interactions . fig2 c is a helical wheel representation of trip residues beginning with inner residue leu 2525 at position d and finishing with the outer residue ala 260 at position d . the helical representation of the putative leucine zipper shows that the position next to the zipper is always hydro - phobic or uncharged , whereas other sides of the wheel contain charged but few hydrophobic residues , suggesting an amphipathic structure that can interact with another helix . to characterize trip further , its expression pattern was examined . northern blot analysis of various mouse tissue rna samples revealed that trip - specific probes detected a − 2 . 1 kb mrna species present in various tissues , but most abundant in testes , thymus and spleen . fig3 a shows the results of a northern analysis of trip mrna in mouse tissues . total rna isolated from various tissues was hybridized with trip - specific probe . the trip - probe hybridized to an approximately 2 . 1 kb mrna , indicated by the arrow . positions of 18s and 28s ribosomal rna are indicated : the amount of total rna loaded in each lane was similar based on the intensity of etbr - stained rrnas . to characterize further the expression of trip in lymphocytes , its expression during lymphocyte proliferation by semi - quantitative pcr was analyzed . for the stimulation of lymphocytes , lymph node cells ( lnc ) were isolated from balb / c mice ( 4 - 6 weeks old ) and cultured on plates coated with anti - tcr ab ( 10 ug / ml ) and anti - cd28 ab ( 1 ug / ml ) for forty - eight hours as described in park et al ., immunity , id . total rna was prepared from unstimulated and stimulated lncs using the total rna isolation kit ( strategene ). first - strand cdna was synthesized from 10 mg of total rna using m - mlv reverse transcriptase and random hexanucleotides following the protocols provided by the supplier ( gibco brl ). quantitative pcr was performed in the linear phase of amplification by testing pcr products from different dilutions of first - strand cdna products . pcr amplification was performed for 35 cycles using 1 of 1000 of the first - strand cdna synthesized above . pcr products were then electrophoresed in a 2 % agarose gel and subjected to southern blot analysis . fig3 b shows the results of expression of traf , c - iap1 and trip during lymphocyte stimulation . as noted above , the cdnas were prepared from lymph node cells stimulated with anti - tcr ab plus anti - cd28 abs for 0 hr ( cont .) and 48 hr ( activ .). the cdnas were then subjected to semi - quantitative pcr using primers specific for mtrip , mtraf1 , and m - c - iap1 as described above . the expression of trip was significantly reduced when lymphocytes were stimulated to proliferate via antigen receptors . this is in contrast to that of other components of the tnfr - traf - signaling complex . for example , the expression of traf1 and c - iap1 was upregulated upon lymphocyte proliferation . these results suggest that the repertoire of signal transducers available in a given cell type can change depending on the state of the cell . the yeast two - hybrid assay was used to determine the structural requirements for the interaction of trip with traf1 or traf2 . in the yeast two - hybrid assay , a mutant trip comprising the n - terminal half of the protein ( residues 1 - 275 in fig2 a ) interacted with trafs whereas a mutant trip lacking the n - terminal ring finger and the coiled - coil domain ( residues 275 - 470 in fig2 a ) failed to interact with the trafs . fig4 a shows the interaction of traf1 or traf2 with the n - and c - terminal domains of trip . expression vectors encoding wild type trip or the indicated deletion mutants of trip fused to the transcription activation domain were cotransformed into yeast with plasmids expressing lexa dna binding domain - traf1 or - traf2 fusion proteins . inter - actions between fusion proteins were scored by measuring b - gal activity of yeast transformants . the “+” indicates average b - gal activity of three independent yeast transformants was higher than 1000 miller units ; and the “−” indicates average b - gal activity of three independent yeast transformants was about 100 miller units , which was similar to that of negative controls ( bait plasmid alone ). further deletion analysis suggested that the putative coiled - coil region of trip mediates the interaction with trafs , since a mutant trip lacking the n - terminal ring finger motif still inter - acted with trafs ( residues 56 - 275 in fig2 a ). in addition , both trip residues 56 - 185 and 186 - 275 interacted with trafs , suggesting that trip contains two independent traf - binding sites within the long coiled - coil domain of the protein . to delineate a region in traf that is required for trip binding , the interaction of trip with various truncation mutants of traf1 or traf2 was determined by the yeast two - hybrid assay or by a transfection - based coprecipitation assay in 293 cells . fig4 b shows the interaction of trip with trafs . expression vectors encoding the n - terminal deletion mutants of traf fused to the lexa dna binding - domain were cotransformed into yeast with plasmids expressing trip fused to the transcription activation domain . interactions between fusion proteins were scored by measuring b - gal activity of yeast transformants as described in conjunction with fig4 a . for the analysis of the c - terminal deletion mutants of trafs a transient transfection - based coprecipitation experiment was used . the indicated c - terminal deletion mutants of trafs were coexpressed with gst - trip fusion proteins in 293 cells . cell lysates were subjected for purification with glutathione - sepharose beads , followed by western blot analysis with anti - traf1 or anti - traf2 polyclonal antibodies as described in conjunction with fig1 . as shown in fig4 b , trip interacted with an n - terminal deletion mutant of traf1 expressing the entire traf domain [ traf1 ( 183 - 409 ) 1 ] but failed to interact with an n - terminal deletion mutant of traf1 expressing only the traf - c domain [ traf1 ( 252 - 409 )]. trip did not interact with a c - terminal deletion mutant of traf1 lacking the traf - c domain [ traf1 ( 1 - 251 )], suggesting that the interaction of trip with traf1 requires the entire traf domain . mutational analysis of traf2 also showed that trip interacts with traf2 through the traf domain . trip did not directly interact with the cytoplasmic domains of tnfr2 or cd30 in a yeast two - hybrid assay . however , since the interaction of trafs with the cognate members of the tnfr superfamily is mediated through the traf - c domain rather than the entire traf domain , it was - important to determine whether trip can indirectly interact with the receptors through trafs . to test this , an ha epitope tagged - trip and gst - fusion proteins with the cytoplasmic domains of tnfr2 ( gst - tnfr2 ) or cd30 ( gst - cd30 ) were co - expressed in 293 cells in the presence or absence of traf . cell lysates were precipitated with glutathione - sepharose beads , and analyzed on western blots with anti - ha , anti - traf1 , and anti - traf2 antibodies . consistent with the yeast two - hybrid assay , trip was not coprecipitated by the gst - tnfr2 or gst - cd30 . fig5 shows the results of 293 cells transiently transfected with the indicated combinations of equal amounts of ha - trip , traf1 , traf2 , gst - cd30 , or gst - tnfr2 expression vectors for thirty - six hours . aliquots of cell lysates were subjected for purification with glutathione - sepharose beads as described in experimental procedures . proteins coprecipitated with gst - fusion proteins were analyzed by western analysis with an anti - ha mab ( 12ca5 ), and anti - traf1 - or anti - traf2 - polyclonal antibodies . in control experiments , gst proteins did not coprecipitate any of the proteins tested . fig5 also shows that cell lysates prior to purification with glutathione - sepharose beads were analyzed by western analysis with anti - trip polyclonal antibodies to reveal that equal amounts of trip was expressed in each case . the positions of molecular mass markers are shown on the left . arrows indicating the positions of traf1 , traf2 or trip are also shown on the left . when traf2 was coexpressed , trip was readily co - precipitated by the gst - tnfr2 . gst - tnfr2 , which does not strongly interact with traf1 oligomer , did not readily co - precipitate the traf1 - trip complex . coexpression of both traf2 and traf1 did not increase the amount of trip coprecipitated with gst - tnfr2 . similar to gst - tnfr2 , gst - cd30 also coprecipitated trip efficiently in the presence of traf2 . although traf1 homo - oligomer can interact with cd30 or trip efficiently in 293 cells , only low level of trip was coprecipitated by gst - cd30 in the presence of traf1 alone . taken together , these results show that trip can be recruited to the tnfr2 or cd30 through the traf2 homo - oligomer . the effect of trip expression on traf2 - mediated nf - kb - dependent reporter gene activation was demonstrated using a transient transfection assay . when overexpressed , trip significantly inhibited traf2 - mediated nf - kb activation . this inhibition was similar to that exerted by overexpression of a dominant negative form of traf2 [ traf2 ( 241 - 501 )]. fig6 a , in its left panel , shows a dose - dependent effect of trip expression on traf2 - mediated nf - kb activation . as before , 293 cells were transfected with 0 . 5 ug of traf2 expression vector together with 0 . 5 ug of p ( kb ) 3 - ifn - luc in the presence of the indicated amount of trip expression vectors . control experiment was transfected with 0 . 5 ug of pcdna3 . 1 control vector and 0 . 5 ug of p ( kb ) 3 - inf - luc . all the transfections included 0 . 25 ug of pcmv - bgal plasmids . after forty - eight hours posttransfection , cell lysates were prepared and used for luciferase assay . all values represent luciferase activities normalized to b - galactosidase activities and are shown as means with their respective sems for representative experiments performed in duplicate . fig6 a , in its right panel , shows that the putative coiled - coil domain of trip is required to inhibit traf2 - mediated nf - kb activation . as before , 293 cells were transfected with 0 . 5 ug of traf2 expression vector together with 0 . 5 ug of p ( kb ) 3 - ifn - luc in the presence of 5 ug of plasmids expressing a dominant negative form of traf2 [ traf2 ( 241 - 501 )], or expressing the indicated trip mutants . for the control experiment , cells were transfected with 0 . 5 ug of pcdna3 . 1 control vector and 0 . 5 ug of p ( kb ) 3 - ifn - luc . all the transfections included 0 . 25 mg of pcmv - bgal plasmids . forty - eight hours posttransfection , cell lysates were - prepared and used luciferase assay . all values represent luciferase activities normalized to b - galactosidase activities and are shown as means with their respective sems for representative experiments performed in duplicate . luciferase activity of the control experiments is shown in the left panel . the inhibition of nf - kb activation by trip required the same domains of trip which mediates the interaction . an n - terminal deletion mutant of trip which lacks the trip - trap interaction domain ( residues 275 - 470 in fig2 a ) failed to inhibit traf2 - mediated nf - kb activation . moreover , a c - terminal deletion mutant of trip containing the n - terminal ring finger motif and the putative coiled - coil domain ( residues 1 - 185 in fig2 a ) was sufficient to inhibit traf2 - mediated nf - kb activation . however , further deletion analysis showed that the ring finger motif of trip was not required for inhibition of traf2 - mediated nf - kb activation because a mutant trip containing only the putative coiled - coil domain ( residues 56 - 275 in fig2 a ) was sufficient to inhibit traf2 - mediated nf - kb activation . overexpression of a mutant trip expressing only the n - terminal ring finger motif failed to inhibit nf - kb activation . these results suggested that the coiled - coil domain of trip ( residues 56 - 275 in fig2 a ) ( seq id no : 3 ) ( seq id no : 4 ) is required for trip - traf interaction and also for inhibition of traf2 - mediated nf - kb activation . since trip associates with the receptor complex , the effect of trip on nf - kb activation induced via tnfr2 or cd30 was also tested . as previously shown , overexpression of chimeric receptors with the extracellular domain of cd8 fused to the cytoplasmic domain of tnfr2 ( cd8 - tnfr2 ) or cd30 ( cd8 - cd30 ) induced nf - kb activation in 293 cells without further crosslinking . this is similar to the activation of nf - kb induced by overexpression of wild - type tnfr2 , cd40 or other chimeric receptors in 293 cells , which will trigger the clustering of signal transducers without additional crosslinking by cognate ligands or antibodies . when trip was coexpressed , the receptor - mediated nf - kb activation was significantly inhibited . fig6 b demonstrates dose - dependent inhibition of tnfr2 — or cd30 - mediated nf - kb activation by trip . the 293 cells were transfected with 1 ug of plasmids expressing the chimeric receptors , cd8 - tnfr2 or cd8 - cd30 , together with 0 . 5 ug of p ( kb ) 3 - ifn - luc in the presence of the indicated amount of trip expression vectors . for the control experiment , cells were transfected with 0 . 5 ug of pcdna3 . 1 control vector and 0 . 5 ug of p ( kb ) 3 - ifn - luc . all the transfections included 0 . 25 ug of pcmv - βgal plasmids . all values represent luciferase activities normalized to β - galactosidase activities and are shown as means with their respective sems for representative experiments performed in duplicate . because nf - kb activation by tnfr2 and cd30 is mediated by traf2 the results are consistent with that trip works as a proximal negative regulator of traf2 - mediated nf - kb activation by members of the tnfr superfamily . since traf2 also mediates nf - kb activation triggered by the tnfr1 - tradd complex , the effect of trip overexpression on tnf - induced nf - kb activation in 293 cells , which is mediated by tnfr1 , was studied . overexpression of trip in 293 cells inhibited tnf - induced nf - kb activation . consistent with this , trip overexpression also inhibited nf - kb activation mediated by tradd overexpression in 293 cells . fig6 c demonstrates that trip overexpression inhibits tnf - induced nf - kb activation . the 293 cells were transfected with 0 . 5 ug of p ( kb ) 3 - inf - luc in the presence or absence of 5 ug of plasmids expressing a dominant negative form of traf2 [ traf2 ( 241 - 501 )], or trip . for the control experiment , cells were transfected with 0 . 5 ug of pcdna3 . 1 control vector and 0 . 5 ug of p ( kb ) 3 - ifn - luc . all the transfections included 0 . 25 ug of pcmv - bgal plasmids . thirty - six hours post - transfection , cells were treated for six hours with 100 pg / ml of either tnf or il - 1 . all values represent luciferase activities normalized to b - galactosidase activities and are shown as means with their respective sems for representative experiments performed in duplicate . fig6 d shows the results on assays that demonstrated trip overexpression inhibits tradd - mediated nf - kb activation . the 293 cells were transfected with 0 . 5 ug of plasmids expressing tradd together with 0 . 5 ug of p ( kb ) 3 - inf - luc in the presence of the indicated amounts of trip expression vectors . for the control experiment , cells were transfected with 0 . 5 ug of pcdna3 . 1 control vector and 0 . 5 ug of p ( kb ) 3 - ifn - luc . all the transfections included 0 . 25 ug of pcmv - bgal plasmids . all values represent luciferase activities normalized to b - galactosidase activities and are shown as means with their respective sems for representative experiments performed in duplicate . whereas traf2 is required for tnf - or tradd - induced nf - kb activation , it is not required for nf - kb activation induced by il - 1 in 293 cells . to test whether trip affects traf2 - mediated nf - kb activation specifically , the effect of trip overexpression on nf - kb activation by il - 1 was also tested as noted above . in contrast to tnf - induced nf - kb activation , il - 1 - induced nf - kb activation was not inhibited by trip overexpression . recent experiments have shown that il - 1 - induced nf - kb activation is mediated by another member of the traf family , traf6 . these results suggest that trip is a specific inhibitor of traf2 - mediated nf - kb activation , rather than a general inhibitor of nf - kb activation . while the invention has been described and illustrated herein by references to various specific material , procedures and examples , it is understood that the invention is not restricted to the particular material combinations of material , and procedures selected for that purpose . numerous variations of such details can be implied as will be appreciated by those skilled in the art .