with reference to fig1 , there is generally shown a cross sectional view of a system incorporating present invention . vessel 1 , containing substance under treatment 2 , is placed on bottom plate 4 of stand 3 . stand 3 consists of plates 4 and 5 connected by columns 6 . columns 6 are formed to support the load induced by cylinder 7 . cylinder 7 is mounted to the top plate 5 by bolts 8 . the cylinder &# 39 ; s piston rod 10 is inserted into neck 11 of vessel 1 . high pressure seal 13 is formed at the end of piston rod 10 . piston rod 10 is in contact with the treated substance 2 . the volume above piston 9 in cylinder 7 is filled with oil and is connected to rotary valve 14 . rotary valve 14 consists of valve housing 15 and rotor 17 . rotor 17 is formed with two sealed chambers 16 . rotor 17 is connected through axle 21 to a motor with an adjustable speed rotation . three openings , “ a ”, “ b ”, and “ c ”, are formed in valve housing 15 . opening “ a ” is connected to the piston side of cylinder 7 . opening “ b ” is connected to tank 18 , while opening “ c ”, through pressure - regulating valve 19 , is connected to high pressure pump 20 . during the rotation of rotor 17 , chambers 16 periodically connect the piston area of cylinder 7 to either low pressure tank port “ b ” or high pressure port “ c ”, thus intermittently changing the pressure on the piston side of cylinder 7 . the oil pressure is regulated by pressure - regulating valve 19 . the pressure applied to the substance depends on the diameter of cylinder 7 and the diameter of piston rod 10 . because piston rod 10 is always in contact with substance 2 , high pressure in substance 2 is generated instantly . by changing the rotating speed of rotor 17 , one can easily control the frequency of the pressure spikes delivered to substance 2 inside vessel 1 . with reference to fig2 , there is generally shown a cross section of another system , incorporating present invention . in this system , however , the rotating valve is replaced by directional control valve 22 with controlling solenoid 23 . depending on its position , valve 22 connects the volume above piston 9 either to the pressure port or to the tank port . controlling solenoid 23 receives an “ on ” or “ of ” signal intermittently , with a controlled frequency , thus delivering pressure pulsations with the same frequency to substance 2 in container 1 . directional control valve 24 is added to facilitate the movement of piston rod 10 in and out of vessel neck 11 . when valve 24 shifts to the right , the system pressure reaches the inlet port of valve 22 and when valve 22 is in its right position , piston rod 10 extends into neck 11 of vessel 1 . when both valves 24 and 22 shift to the left , piston rod 10 retracts from the neck 11 . with reference to fig3 , there is generally shown a cross section of another system incorporating present invention . in this system , however , air pressure is incorporated to produce the pulsating pressure spikes in vessel 1 with regulated frequency . valve 22 is controlled by solenoid 23 as shown in fig2 . if the desired result can be achieved using of lower pressure amplitudes , this system will provides a simple and cost effective solution . with reference to fig4 , there is generally shown a cross section of another system incorporating present invention . this system is similar to the system in fig1 , except that pumps 25 and 26 , tanks 27 and 28 , and directional control valve 29 is added to provide the automatic loading and unloading of treated product 2 . pump 25 loads the untreated product from tank 28 into vessel 1 , while pump 26 unloads the treated product from vessel 1 to tank 27 . directional control valve 29 directs the flow of the product either from tank 28 to vessel 1 or from vessel 1 to tank 27 . with reference to fig5 , there is generally shown a cross section of another system incorporating present invention . in this system , however , pressure pulsations are produced by rotating cam 31 , which is in contact with rod 35 . vessel 1 is placed on spring - loaded platform 36 to prevent rotating cam 31 from breaking . cam 31 is driven by motor 32 through gear box 33 . the frequency of pulsations is regulated either by the speed of motor 32 or by switching gears in gearbox 33 . the amplitude of pressure pulsations in this system is regulated either by the stiffness of elastic elements 34 or by the contact area between rod 35 and substance 2 . with reference to fig6 , there is generally shown a cross section of another system incorporating present invention . this system is similar to that of fig5 . however , the rotating cam in this system is replaced by a number of rollers 38 formed on the outer diameter of wheel 37 . this is done to increase the frequency of pressure pulsations in substance 2 . the amplitude of pressure pulsations is controlled by the contact area between push rod 39 and substance 2 . to facilitate the use of push rods with different contact areas in one container , insert 40 is formed inside neck 11 and secured by clamp 41 . inserts 40 always have the same outer diameters while the inner diameters are formed to accommodate push rods with different contact areas . by using various insert - push rod combinations , one can regulate the amplitude of pressure pulsations generated in substance 2 . with reference to fig7 , there is generally shown a cross section of another system incorporating present invention . in this system , however , high frequency pressure pulsations are produced by ultrasonic vibrator 47 . ultrasonic vibrators are frequently used for various applications , including ultrasonic welding and the cleaning of metal parts . the frequency and amplitude of vibrations in these devices can be regulated . ultrasonic device 46 is mounted on rigid column 44 , which is mounted on plate 49 . vibrating horn 48 is attached to ultrasonic device 46 . the distance between vibrating horn 48 and plate 49 can be adjusted by moving the ultrasonic device 46 up or down on guide rail 45 . once vessel 1 is placed on plate 49 , ultrasonic device 46 is brought down until horn 48 is in contact with the top of rod 39 , which is in contact with substance 2 . the initial pressurization of substance 2 is achieved through pressure regulating valve 43 and pump 25 . after the setup is completed , horn 48 starts vibrating , this , in turn , generates pressure pulsations in substance 2 with a frequency equal to the vibration frequency of horn 48 . neck 42 facilitates the removal of trapped air from vessel 2 . with reference to fig8 , there is generally shown a cross section of another system incorporating present invention . however , this system is formed to be used for the treatment of solid products . solid product 52 is vacuum - packed in plastic wrap 53 and then placed inside vessel 1 . product 52 is suspended inside vessel 1 and surrounded by liquid 2 . vessel 1 is sealed by cover 50 , which is secured to vessel 1 by clamp 51 . ultrasonic device 46 generates pressure pulsations with a set frequency in liquid 2 which are transferred through liquid 2 to product 52 . ultrasonic vibrator 47 is attached to upper plate 5 . due to this setup , the size of vessel 1 is not limited by the size of column 44 and the quantity of substance treated in vessel 1 in one treatment cycle can be increased . with reference to fig9 , there is generally shown a cross section of another system incorporating present invention . in this system , cooling jacket 54 is formed around vessel 1 to control the temperature of substance 2 during the pulsating pressure treatment . a cooling liquid is then pumped from tank 56 by pump 55 through heat exchanger 57 into cooling jacket 54 and back into tank 56 . with reference to fig1 , there is generally shown a cross section of another system incorporating present invention . this system is similar to the system described in fig9 ; however , its cooling arrangement is made to be more efficient . in this system , rather then the cooling jacket controlling the temperature of substance 2 , substance 2 itself , is circulated by pump 58 through heat exchanger 57 during or between pressure pulsation treatments . with reference to fig1 , there is generally shown a cross section of another system incorporating present invention . in this system , however , cylinder 7 alternates between producing instantaneous pressure pulsations in substance 2 , while the substance is locked between valves 29 and 61 , and pumping parts of the substance 2 from container 1 into container 59 through nozzle 60 when valve 61 is open . initially , control valve 22 works with a set frequency in an oscillating mode that produces pressure pulsations needed to treat substance 2 . thereafter , treatment valve 22 shifts to the right , permitting the free flow of oil under pressure to the piston side of cylinder 7 . valve 61 , subsequently , opens after that , permitting the substance to escape from vessel 1 into vessel 2 . valve 24 , through which pressure was delivered to valve 22 , stays in its rightward position . system pressure , through valves 22 and 24 , acts on the piston side of cylinder 7 . piston rod 10 extends , pushing a portion of substance 2 from vessel 1 , through valve 61 and nozzle 60 , into vessel 59 . after piston rod 10 extends fully , valve 61 shifts to block the flow from vessel 1 into vessel 59 , and valve 22 shifts to the left , connecting the piston side of cylinder 7 to tank 18 . at this moment , valve 29 shifts to the right and the untreated substance moves from storage tank 28 to vessel 1 by pump 25 . under pressure from substance 2 , cylinder rod 10 retracts . after cylinder rod 10 retracts fully , valve 29 closes and valve 22 starts oscillating again . in this system , the automatic unloading of vessel 1 is done without additional pumps , and valves and the system can be adapted to combine different types of substance treatments in one smooth process . each time the treated portion of substance 2 is pushed out from the bottom of vessel 1 , an untreated substance is added at the top of vessel 1 , with minimal mixing between them . by the time the added substance reaches the bottom of vessel 1 it will have been subjected to a number of high pressure pulsation treatments . nozzle 60 will provide additional treatment to substance 2 if necessary , and other types of treatments , such as “ cold vapor ” and vacuum , can also be incorporated inside vessel 2 . with reference to fig1 , there is generally shown a cross sectional view of another system incorporating present invention . in this system , however , a single device generating pressure pulsations is used to consecutively treat multiple containers moving on a conveyor belt . containers 66 are filled with the substance and placed on a conveyer 68 . cover 65 with small opening 64 is used to minimize spillage and contamination of the substance . the conveyer moves intermittently and places each container 66 under device 69 for a set period of time . device 69 is comprised of sealing element 70 , which contains a number of openings , and cylinder 71 . cylinder 71 is formed to move sealing element 70 up and down from containers cover 65 . device 69 is formed to bring pressure pulsations from pressure pulsation generator 72 to the substance in containers 66 . any pressure pulsation generating device that has been described in the present invention can be employed in this application . the system operates in the following sequence : after container 66 is placed under sealing element 70 , cylinder 71 extends and brings sealing element 70 in contact with cover 65 to seal opening 64 ; valves 67 and 65 shift to the “ open ” position and pump 25 starts pumping the product from tank 28 to pressurize the substance in container 66 before the pressure pulsation treatment begins ; valve 67 shifts to a closed position and pressure pulsation generator 72 starts generating pressure pulsations to treat the substance in vessel 66 ; after the treatment , valve 65 closes and valve 67 opens , allowing double rotating pump 25 to suck back the excess product and prevent spillage ; valve 67 closes again , sealing element 70 is lifted and vessel 66 is moved out of the way ; the next in line vessel 66 is placed under device 69 . with reference to fig1 , there is generally shown a cross sectional view of another system incorporating present invention . in this system , however , an air - hydraulic buster is incorporated to produce high pressure pulsations in treated substances . vessel 1 , containing product 2 , and hydraulic cylinder 77 are shown in a horizontal position . blocks 71 and 72 are mounted in pockets made in mounting plate 74 to support cylinder 77 and vessel 1 during operation . plugs 78 and 79 are needed to close the openings in vessel 1 through which substance 2 can be loaded and unloaded . air valve 22 brings air pressure to piston 76 , which is placed inside air - hydraulic booster 75 . air valve 22 is controlled by an on - of pulsating signal . through the action of piston rod 81 on the oil locked in cavity 80 , low air pressure pulsations are transformed to high pressure hydraulic pulsations acting on cylinder 77 . since insignificant travel of piston rod 82 is required to generate pressure pulsations in substance 2 , which is locked under pressure in vessel 1 , a small air - hydraulic booster is sufficient to do the job . air pressure is readily available in a laboratory or in industrial environment so this approach can provide a low cost and practical solution to generating high pressure pulsations with regulated frequency . with reference to fig1 and fig1 a , there is generally shown a cross sectional view of another system incorporating present invention . in this system , however , two processes are involved in the treatment of liquid substances ; homogenization and pressure pulsation with a set frequency are combined in the same vessel . homogenization is a process used in the production of many foodstuffs . high pressure pulsations is this system are delivered by plunger 35 . delivering pressure pulsations to substance 2 in container 1 can be accomplished through plunger 35 by any of the devices described above . in the arrangement shown in fig1 , pressure pulsations are delivered by ultrasonic device 44 and vibrating horn 48 . homogenization of substance 2 is performed by cylinder 77 . piston rod 82 of cylinder 77 is attached to rod 91 through bushing 90 . rod 91 , through an opening in closure 83 of vessel 1 , is connected to piston 84 , which is formed with small openings 85 . pump 25 fills vessel 1 with untreated substance from tank 28 through valve 92 . once vessel 1 is filled , valve 92 closes . at this point in the cycle , rod 82 is fully retracted . piston 84 is pushed against sealing ring 86 , which is retained in a groove of sliding ring 87 , thus preventing the substance from escaping through openings 85 . the process of killing bacteria in substance 2 begins when ultrasonic device 44 starts generating pressure pulsations in vessel 1 . after the ultrasonic treatment is done , the homogenizing process begins . piston rod 82 of cylinder 77 expands , pushing piston 84 through substance 2 . under the pressure in front of piston 84 , retainer ring 87 is pushed back against the step formed on rod 91 , thus allowing substance 2 to escape to the back of piston 84 through openings 85 . when piston 84 reaches its far right position , valve 94 opens and rod 82 of cylinder 77 begins to retract . retaining ring 87 with sealing ring 86 is being then pushed by inertia and pressure , against the back surface of piston 84 , thus preventing substance 2 from escaping to the front of piston 84 through openings 85 . thereafter substance 2 moves to tank 27 through valve 94 . the described system does not only allow the combination of pulsating pressure treatment and homogenization of the substance in one process , but also allows automatic unloading of the treated substance after treatment , which is another useful feature in mass production . with reference to fig1 , there is generally shown a cross sectional view of another system incorporating present invention . in this system , however , high pressure pulsation and homogenization processes are combined and accomplished by one cylinder . cylinder 77 is controlled by valves 22 and 24 . this system works in the following sequence : as vessel 1 is filled with the untreated substance from storage tank 28 through valve 92 by pump 25 , piston 84 is pushed to its extreme leftward position . when vessel 1 is filled with substance 2 , under a pressure that is set by control valve 28 , valves 92 and 95 close ; valve 22 receives an “ on ” or “ off ” signal at a set frequency , generating pressure pulsations in cylinder 77 , which are transferred to substance 2 in vessel 1 . with each pressure pulsation , piston 84 moves a small distance to the right , forcing a small portion of substance 2 through openings 85 to the rear of piston 84 , into the space that is formed by the rightward movement of piston 84 . with this approach , the homogenization process is combined with the process of destruction of bacteria by high pressure pulsations . valve 94 is utilized at the initial stage of the treatment process in order to equalize the duration of treatment of the substance located immediately in front of piston 84 , with that of the substance located further away from piston 84 , which spends more time in vessel 1 prior to efflux from the vessel . after piston 84 travels a set distance forward , valve 94 is shifted to its leftward position , piston rod 82 retracts , and the substance is returned from the space behind piston 84 to that in front of piston 84 , where it undergoes additional pressure pulsation treatment . as soon as the initial equalization cycle is complete , valve 94 moves permanently to its rightward position , connecting the space behind piston 84 to valve 95 , which is in its closed position . at this point , piston 84 starts to move rightward again , delivering pressure pulsations to substance 2 . after it traverses some set distance ( for example , one third of its total stroke ), valve 95 opens , piston 84 is moved back to its starting position , and treated substance that had accumulated in the space behind piston 84 ( as described above ) is pushed into finished product tank 27 . meanwhile , untreated substance is added to vessel 1 from tank 28 through open valve 92 by pump 25 , and the process resumes . in the present invention , several different methods of generating the pulsating pressure for the purpose of immobilizing the undesired microorganisms in the liquid substances are described . these systems can also be fitted with interchangeable components , including different size actuators , cylinders and inserts , to provide a variety of pressure and frequency combinations . the variety of pressure and frequency combination is needed in conducting the research into the combination of the parameters that are most effective for use in production , that achieve the best results in the most economical and efficient way . various possible embodiments , forms and modifications of the invention , coming with the proper scope and spirit of the appended claims , will , of course , readily suggest themselves to those skilled in the art . thus , while what has been described is at present considered to be preferred embodiments of the invention , it will be apparent to those skilled in the art that various changes and modifications can be made therein , without departing from the invention , and it is therefore the aim in the appended claims to cover all such changes and modifications as fall within the true spirit of the invention , and it is understood that , although the preferred form of the invention has been shown , various modifications can be made in the details thereof , without departing from the spirit as comprehended by the following claims .