the present invention discloses a method for non - fluorescence higher harmonic generation ground state depletion super - resolution microscopy , it includes the following steps : providing an organic material unit , focusing excitation light and ground state depletion light , generating a higher harmonic signal , performing ground state depletion and performing microscopic imaging . with the implementation of the present invention , the stimulated electrons of the organic material remains majorly on the singlet state or the triplet state , instead of the ground state , to provide modulation of the spatial distribution of the non - fluorescence signal , and make sted microscopy applicable to non - fluorescence signals to promote the resolution of the images .