a method of generating a chronic myelogenic leukemia acquired chemoresistant culture model is provided . such a method may comprise providing a naïve blast crisis cml cell line ; administering / contacting the cell line with a mutation - inducing dose of imatinib ; maintaining a culture of the treated cell line for a period of time until the treated cell line relapses and repopulates the culture ; and determining the repopulated cell culture is a cml acquired chemoresistant cell line by detecting a bcr - abl mutation , wherein the acquired chemoresistance is achieved by a bcr - abl mutation .