a novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution . the device provides a gasket - and - cover - type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles . one or more of the inner walls that define the chamber contain bubble - rupturing elements that extend into the chamber and terminate in sharp edges . they are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement . their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate . the sensitivity and consistency of the hybridization reaction is significantly increased .