a biomedical testing sheet comprises : a substrate with calligraphy paper material , wherein the substrate comprises a wax pattern layer , which is a part of a surface of the substrate coated by a waxy material , and penetrated and diffused by the waxy material , and the wax pattern layer comprises one or more carrying units for carrying one or more droplets of liquids to be measured . the lotus effect substantially prevents diffusion of the liquids and reagents on the carrying units , and reduces the required amount of liquids to be measured and the amount of agent .