a method for determining transduction efficiency of baculovirus includes : providing a recombinant baculovirus , in which the recombinant baculovirus includes an inducible promoter and a reporter gene positioned downstream the inducible promoter ; adding the recombinant baculovirus to incubating environment of a mammalian cell for transduction ; adding an inducer to promote expression of the reporter gene in the mammalian cell ; and analyzing the percentage of the mammalian cell expressing the reporter gene to determine the transduction efficiency of the recombinant baculovirus . the method provides the ability to quantitatively analyze baculovirus transduction and is a simple and faster quantitative method applicable in transduction and other research study by including an inducible promoter and a reporter gene and thus prevents from imposing excessive metabolic burden to the cells . a method for determining virus dosage of baculovirus applied in genetic therapy is also disclosed .