the present invention is directed to a method of acacia mangium regeneration through organogenesis and a method of genetic transformation of acacia mangium . the method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants ; adventitious bud induction followed by pinnate leaf and bud elongation and eventually , elongated shoots were induced to root . based on the regeneration system , a marker gene gus under cauliflower mosaic virus promoter was introduced to acacia mangium via agrobacterium infection . gus staining in the regenerated plants and southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene .