in some embodiments , a system may function to transfer samples in a controlled environment . the system may include a sample container configured to convey a sample from a first device to a second device . the first device may be under pressure and the second device may be under vacuum . the second device may include a load chamber which functions to accept the sample from the sample container . the second device may include a pump chamber coupled to the load chamber using a conduit such that the pump chamber is in fluid communication with the load chamber as required . the second device may include a high vacuum pump coupled to the pump chamber . the second device may include a vacuum pump coupled to the pump chamber through the high vacuum pump in sequence . the second device may include an orifice sized to significantly restrict the flow of fluids through the conduit coupling the pump chamber to the load chamber , wherein the orifice is configured to allow for a transition from a viscous into a molecular flow .