a proess , and a composition of matter utilized therein , to obtain transformation of the glochidia of freshwater mussels to the juvenile stage . in nature , glochidia normally transform while as parasites on fish . the instant artificial process involves the use of cell culture and bacteriological techniques to best assure environmental integrity during the protracted culture period . the composition of the media includes a combination of the blood plasma of fish and commonly available tissue culture fluids and inorganic salts . a nonspecific component of fish blood plasma in combination with the media , supra , was determined to be essential for glochidial transformation to juveniles .