the present invention relates to a method for identifying a dna breakpoint and agents for use therein . more particularly , the present invention provides a method for identifying a gene translocation breakpoint based on the application of a novel multiplex dna amplification technique . the method of the present invention facilitates not only the identification of the breakpoint position but , further , enables the isolation of the dna segment across which the breakpoint occurs . this provides a valuable opportunity to conduct further analysis of the breakpoint region , such as to sequence across this region . the method of the present invention is useful in a range of applications including , but not limited to , providing a routine means to characterize the gene breakpoint associated with disease onset in a patient and thereby enable the design of patient specific probes and primers for ongoing monitoring of the subject disease condition . in addition to monitoring the progression of a condition characterized by the existence of the breakpoint , there is also enabled assessment of the effectiveness of existing therapeutic drugs and / or new therapeutic drugs and , to the extent that the condition is a neoplasm , prediction of the likelihood of a subject &# 39 ; s relapse from a remissive state .