a portable reverse isolation box for protecting an object from the environment and / or protecting a user from the object . the prib includes a housing comprising a container and a lid , wherein the container has structural integrity and at least a portion of the lid is transparent , and further wherein the housing forms an airtight and watertight isolation bay when the lid is mounted on the container ; a port formed in the container ; and a disposable flexible element mounted in the port in an airtight and watertight relationship , the flexible element being capable of assuming a concave configuration so as to permit a user to manipulate the object within the isolation bay without opening the lid .