the present invention is directed to a system and method for cross - talk cancellation for multi - lane fluorescence detectors . the invention may be implemented in accordance with a variety of systems , including systems for multi - capillary electrophoresis . the present invention is based on a special calibration procedure for determination of a channel cross - talk matrix and enables an accurate separation of the fluorescence emitted from individual capillary lanes . the proposed method for cross - talk calibration and removal is very useful for design and development of multi - lane single photon counting detection systems .