arrangement for microscopic observation and / or detection of a sample that is at least partially transparent by way of a microscope objective , whereby an illumination of the sample outside the objective is carried out from at least from one side at an angle to the optical axis of the objective and the illumination light is focused on the sample with a smaller aperture than that of the viewing objective and that a coupling of the illumination light over a beam splitter , preferably in the objective pupil , is carried out for coupling , at its circumference , slightly expanding transmitting or reflecting areas for steering the illumination light to the sample , but otherwise is designed so that it is reflecting or transmitting for the sample light on the rest of the area .