methods of producing stem cell conditioned media to treat mammalian injuries or insults . in at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure , the method comprises the steps of culturing at least one stem cell in a first cell culture medium , replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium , and collecting a quantity of the second cell culture medium after a culture duration , wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury . in another embodiment , the step of culturing comprises culturing the at least one stem cell in egm2mv .