the invention is directed to the enhanced expression and purification of lipoxygenase enzymes . these enzymes are of wide - spread industrial importance , often produced in heterologous microbial systems . preferably , the lipoxygenase produced by the methods of the invention is a plant - derived enzyme and expressed at high - levels in a microbial system that includes a protease - deficient host and one or more chaperone expression plasmids . the invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes . the invention is also directed to methods of manufacturing bread and other food and also non - food products with lipoxygenase manufactured by the methods of the invention .