the systems and methods herein involve the use of an automated , high - throughput system that utilizes pressure to transfer a fluid medium containing an analyte . in preferred embodiments , the sample is delivered to an analytical device . the sample can comprise one or more analytes , e . g ., solvents , solutes , or particles , including rare cells . the systems are designed to minimize contact with potentially hazardous , fragile , or valuable samples . the systems allow for the dilution , mixing , and introduction of the fluid medium to an analytical device , followed by possible further analysis or sample manipulation . the systems and methods herein allow for partial or substantially complete depletion of a sample container to avoid wasting rare analytes or prevent retention of desired material in a first container .