there is provided a system for protection / isolation against infections or disease - bearing persons including an enclosure defining at least two chambers , an isolation chamber having at least one entering and exiting closable opening , at least one ambient air inlet and at least one air outlet connectable to a blower / filter , and an airlock chamber juxtaposed the opening having an access and egress closable aperture , means for forming under - pressure in the isolation chamber and airlock chamber , and at least one biological filter operationally connected to the means for forming under - pressure . a method for protecting / isolating against infection or disease - bearing persons , is also provided .