a method for the assessment of neuromuscular function by motor unit number estimation , comprising : determining and controlling stimulation and data acquisition process via pre - configured electrode array so as to acquire stable and less uncertainty mu responses ; pre - processing acquired mus responses so as to attenuate noise , determine mus activity region , and improve processing speed and accuracy ; minimizing alternation effects by globally searching and comparing smups ; eliminating alternation effects by identifying alternating mus directly ; and computing and reporting mune results , as well as the statistical description of these mun estimates to evaluate its robustness .