disclosed is apparatus and method for controlled surface plasmon resonance analysis having a surface plasmon resonance sensor with a derivatized surface plasmon layer in optical communication with the sensor , derivatizing the surface plasmon layer and placing an analyte detection chamber in fluid communication with the derivatized surface plasmon layer . the chamber is adapted for the generation of a molecular interaction bias across the chamber . a conjugate is provided between an analyte and a bias responsive element , wherein the analyte is reactive with the derivatized surface plasmon layer and the bias responsive element changes the response of the analyte to the molecular interaction bias . a conjugated analyte may be introduced into the chamber , generating a molecular interaction .