a cleavage apparatus for obtaining a free peptide after assembly of a peptide on a solid support by solid - phase peptide synthesis equipped with a cleavage cocktail transport line installed between the column for cleavage containing a peptidyl resin and a cleavage cocktail supply bottle , through which a cleavage cocktail is supplied from the bottle to the column and recovered into the cleavage cocktail supply bottle from the column . this apparatus can provide easy , safe , rapid and reliable cleavage in a large scale peptide synthesis .