 <p>You need the following in your <code>app.yaml</code>:</p> <pre><code>- url: /account script: account/account.py - url: /game script: game/game.py - url: .* script: main.py </code></pre> <p>BTW, I suggest you try to forget backslashes (characters like this: \ ) -- think <em>normal</em> slashes (characters like this: / ). Backslashes are a Windows anomaly (and mostly unneeded even there -- Python will happily accept normal slashes in lieu of backslashes in filepaths), not used as path separators in URLs nor on Unix-y operating systems (including Linux and MacOSX). I mention this because you speak of "requests that go to \account\ and \game\ respectfully" and there are no such things -- no request goes to a path with backslashes, it will always be <em>forward</em> slashes.</p>