 <p>You can use the <a href="http://effbot.org/imagingbook/image.htm#tag-Image.Image.crop" rel="nofollow">crop</a> method from PIL (or PILLOW)</p> <pre><code>from PIL import Image import glob for filename in glob.glob('yourpath/*.tiff'): im = Image.open(filename) w, h = im.size im.crop((0, 30, w-60, h-30)) </code></pre>