A car hit two bikes in Mauldeth Road West, in Withington, Manchester, at about 22:15 GMT on Sunday.
A 24-year-old cyclist was taken to hospital where he later died and the other bike rider, a man aged 21, was treated for minor injuries.
The 61-year-old driver was arrested on suspicion of causing death by dangerous driving. He remains in police custody.
A man has been arrested after a cyclist was killed in a crash with another bike and a car.