The victim, from Redruth, was found unconscious with head injuries in the Chapel Street area at 02.45 BST on Sunday.
He died in hospital on Monday.
Connor Hammond, 21, of Trenoweth Estate, North Country, and Liam Laing, 21, of Brookside, Chacewater, are due at Truro Magistrates Court on Tuesday.
Detective Chief Inspector Mike West, from Devon and Cornwall Police, said: "Offences like this are exceedingly rare in this area.
"We would like to speak to anyone who witnessed a disturbance outside The Zone night club and anyone who was in the area between 2.30am and 3.30am on Sunday."
Two men have been charged with the murder of a 20-year-old man after a "disturbance" outside a nightclub, police said.