James Alabi broke the deadlock in spectacular fashion for Chester with an overhead kick in the 29th minute, smashing home from Blaine Hudson's flick-on.
Simon Walton equalised for the hosts from the penalty spot after 52 minutes, sending Alex Lynch the wrong way after Johnny Hunt brought down Connor Brown in the box.
The draw extends Chester's unbeaten run to six matches and they are up to seventh in the table, while Guiseley remain 22nd.
Report supplied by the Press Association.
Match ends, Guiseley 1, Chester FC 1.
Second Half ends, Guiseley 1, Chester FC 1.
Danny Lowe (Guiseley) is shown the yellow card.
Substitution, Chester FC. Jordan Chapell replaces Craig Mahon.
Substitution, Chester FC. Wade Joyce replaces Kane Richards.
Goal!  Guiseley 1, Chester FC 1. Simon Walton (Guiseley) converts the penalty with a.
Second Half begins Guiseley 0, Chester FC 1.
First Half ends, Guiseley 0, Chester FC 1.
Goal!  Guiseley 0, Chester FC 1. James Alabi (Chester FC).
Substitution, Guiseley. Marcus Williams replaces Robert Atkinson.
First Half begins.
Lineups are announced and players are warming up.
Guiseley salvaged a 1-1 draw against Chester to boost their National League survival hopes at Nethermoor Park.