Under Tom Eaves, 11-1 Brando was comfortable despite late pressure from the Richard Fahey-trained Growl.
It was a fourth win in the Ayr Gold Cup for trainer Kevin Ryan in the past 10 years, following wins for Advanced (2007), Our Jonathan (2011) and Captain Ramius (2012).
Hoof It claimed third place, with G Force making a late dash for the line to finish fourth.
Brando won the Ayr Gold Cup, edging 4-1 favourite Growl into second place.