Darren Osborne, from Cardiff, appeared at the Old Bailey by video link from Belmarsh prison.
He is accused of killing Makram Ali, 51, from Haringey and attempting to kill others in the attack in Finsbury Park on  June 19.
Mr Osborne, 47, spoke only to confirm his identity during the short hearing.
He will next appear at the same court on 20 July for a preliminary hearing.
Worshippers were leaving evening prayers when the attack happened. Nine people were taken from the scene to hospital.
A man has appeared in court charged with murder and attempted murder after a van was driven into worshippers near a mosque in north London.