Paint was thrown at the property in Roden Street, causing damage to a window, some time between 21:00 BST on Friday and 02:30 BST on Saturday.
The house was empty at the time and no-one was injured.
Insp James Murphy appealed to anyone with information about the attack to contact officers on the non-emergency number 101.
An overnight attack on a house in west Belfast is being treated as a homophobic hate crime.