The 32-year-old man was found at a property in Nettleton Road in Gloucester in the early hours of Thursday. He died later in hospital.
Gloucestershire Police said three women and three men were arrested.
Four have been held on suspicion of murder and two on suspicion of assisting an offender.
Six people have been arrested in connection with death of a man who was found with stab wounds at a house in Gloucestershire.