The man was riding a black Kawasaki motorcycle when it was in a crash with a grey Mitsubishi Shogun on the B4319, Pembroke to Maidenwells road.
Dyfed-Powys Police said the road was closed for examinations and inquiries to take place, and will remain so until further notice.
Officers are appealing for anyone who witnessed the collision, or anyone with any information, to contact them.
A motorcyclist has died in a collision with a 4x4 vehicle in Pembrokeshire.