Many of the couples had met just days before and had been matched by Church authorities before Saturday's ceremony in Gapyeong, north-east of Seoul.
The Church's mass weddings - which take place regularly - began in the 1960s.
Founded by late Sun Myung Moon, the Church has been accused of brainwashing its followers, a claim it denies.
Sung Myung Moon died in September 2013 aged 92.
He is revered by his followers, who are often referred to as "Moonies". Critics have described him as a charlatan.
About 3,000 couples from around the world have taken part in a mass wedding at the South Korean headquarters of the controversial Unification Church.