The new positions at PRA Health Sciences have been backed by £990,000 funding from the Welsh government.
The US company has had a base in the city since 1996 and already employs about 200 people.
Economy Minister Edwina Hart said: "I am delighted that PRA is further expanding its operational centre in Swansea creating high skilled, graduate level employment.
"It is also a superb endorsement of Wales as a great business location."
The operation in Swansea collects, analyses and interprets data from clinical trials - with 5,000 employees in more than 50 offices across the world.
A clinical research company is creating 87 new graduate jobs in Swansea.