The trailer gave a taster of the adaptation of the popular erotic trilogy is set to a new version of Beyonce's hit single Crazy in Love.
The film stars Calvin Klein model-turned-actor Jamie Dornan as billionaire Christian Grey.
Dakota Johnson is shy student Anastasia Steele.
The preview beat Teenage Mutant Ninja Turtles which got 31 million views in March according to figures from technology firm Zefr.
Directed by Sam Taylor-Johnson and co-produced by author EL James, the film is due for release in UK cinemas on Valentine's Day 2015.
The S&M trilogy has sold more than 100 million copies, making it one of the biggest and fastest-selling book series of all time.
A spokesperson from YouTube said that each click on the video is a view and the numbers are calculated globally.
YouTube have "sophisticated technology to count views consistently in order to prevent abuse of the system".
Follow @BBCNewsbeat on Twitter and Radio1Newsbeat on YouTube
The film trailer for Fifty Shades of Grey is the most watched YouTube preview of the year reaching more than 36 million views in less than a week.