Brendon Constant, 87, of Richmond Road, Wisbech, is charged with murdering Jean Constant, 86, on August 22 last year.
Mrs Constant's body was found after police investigated reports of concern relating to Poppyfields care home in Chapman Way, Eynesbury.
A post-mortem examination concluded Mrs Constant died from plastic bag asphyxia in association with heart disease.
Mr Constant appeared before Cambridge Magistrates' Court then Cambridge Crown Court.
He was remanded and will appear before the court for a plea and trial management hearing on 3 May.
A man has appeared in court charged with the murder of his wife.