Ahead of their arrival at his residence, Mr Netanyahu again denied any wrongdoing.
He told the media and political rivals to "hold off partying", adding: "Nothing will happen, because there is nothing."
Israeli media have reported allegations that he has received significant gifts or "favours" from businessmen.
Investigators questioned the PM at his central Jerusalem residence for about three hours, the Jerusalem Post newspaper says.
Mr Netanyahu told his Likud party legislators earlier on Monday:  "We hear all the media reports. We see and hear the festive spirit and atmosphere in television studios and in the corridors of the opposition.
"I want to tell them to wait for the celebrations. Do not rush. I told you and I repeat: There will be nothing because there is nothing. You will continue to inflate hot air balloons and we will continue to lead the state of Israel."
However, Ynet quoted the leader of the Zionist Union, Isaac Herzog, as saying: "This isn't a happy day. This is a hard day for the state of Israel. We are not gloating."
Opponents of Mr Netanyahu have called for an investigation into his affairs following a series of scandals in recent months - none of which have resulted in charges.
Last month, an investigation was opened into the purchase of new submarines from Germany, after it was claimed that Mr Netanyahu's lawyer represented the company during negotiations.
Earlier this year, convicted French fraudster Arnaud Mimran claimed he had donated hundreds of thousands of euros to Mr Netanyahu's 2009 election campaign - something the prime minister denies.
He has also been accused of wasting public money, including $127,000 (£102,000) on a customised private bedroom on a single flight to the UK.
Similar allegations have followed the prime minister since his original term in office two decades ago.
In 2000, Israeli police recommended that criminal charges be brought against Mr Netanyahu and his wife, Sara, after an eight-month investigation into whether he had kept official gifts that should have been handed over to the state after he left office.
At the same time, they were also accused of charging the government for the services of a contractor who did private work for them.
All those charges were dropped due to insufficient evidence.
Israeli police have questioned Prime Minister Benjamin Netanyahu as part of an inquiry into corruption allegations.