Newcastle winger Rolando Aarons, Arsenal's Isaac Hayden, currently on loan at Hull, and Swansea midfielder Matt Grimes, on loan at Blackburn, have all been included for next week's game.
"It's an opportunity for new players," Southgate said.
"But also a lot of work for us to do to get the harmony within the group."
England, currently top of group nine and three points clear of Norway and Switzerland, will be without Duncan Watmore, Nathaniel Chalobah and Nathan Redmond who are all out injured.
Goalkeepers: Pickford (Sunderland), Walton (Brighton & Hove Albion), Wildsmith (Sheffield Wednesday)
Defenders: Chambers (Arsenal), Chilwell (Leicester City), Galloway (Everton), Hause (Wolves), Iorfa (Wolves), Stephens (Southampton; on loan at Coventry), Targett (Southampton)
Midfielders: Baker (Chelsea; on loan at Vitesse Arnhem), Grimes (Swansea; on loan at Blackburn), Hayden (Arsenal; on loan at Hull City), Loftus-Cheek (Chelsea), Swift (Chelsea; on loan at Brentford), Ward-Prowse (Southampton)
Forwards: Aarons (Newcastle United), Akpom (Arsenal; on loan at Hull City), Gray (Leicester City), Ibe (Liverpool), Solanke (Chelsea; on loan at Vitesse Arnhem), Wilson (Manchester United; on loan at Brighton & Hove Albion)
England Under-21s manager Gareth Southgate has handed three players a debut call up ahead of their European Championship qualifier in Switzerland.