Jacobson, 29, had started the first four League Two games before being injured in training prior Saturday's 2-0 home defeat by Colchester.
Wycombe have also confirmed keeper Scott Brown will be out for 12 weeks.
Myles Weston, Paris Cowan-Hall, Max Muller and Luke O'Nien are also currently injured for the Chairboys.
Wycombe Wanderers left-back Joe Jacobson is set to be out for 10-12 weeks, leaving more than a third of the squad in the treatment room.