a carrier is a transmembrane protein that moves molecules across the membrane through
Membrane transport protein A membrane transport protein (or simply transporter) is a membrane protein[1] involved in the movement of ions, small molecules, or macromolecules, such as another protein, across a biological membrane. Transport proteins are integral transmembrane proteins; that is they exist permanently within and span the membrane across which they transport substances. The proteins may assist in the movement of substances by facilitated diffusion or active transport. The two main types of proteins involved in such transport are broadly categorized as either channels or carriers. The solute carriers and atypical SLCs[2] are secondary active or facilitative transporters in humans.[3][4]
Membrane transport protein Active transport is the movement of a substance across a membrane against its concentration gradient. This is usually to accumulate high concentrations of molecules that a cell needs, such as glucose or amino acids. When the lipid bilayer is impermeable to the molecule needing transport, active transport is also necessary. If the process uses chemical energy, such as adenosine triphosphate (ATP), it is called primary active transport. Secondary active transport involves the use of an electrochemical gradient, and does not use energy produced in the cell.[10] Unlike channel proteins which only transport substances through membranes passively, carrier proteins can transport ions and molecules either passively through facilitated diffusion, or via secondary active transport.[11] A carrier protein is required to move particles from areas of low concentration to areas of high concentration. These carrier proteins have receptors that bind to a specific molecule (substrate) needing transport. The molecule or ion to be transported (the substrate) must first bind at a binding site at the carrier molecule, with a certain binding affinity. Following binding, and while the binding site is facing the same way, the carrier will capture or occlude (take in and retain) the substrate within its molecular structure and cause an internal translocation so that the opening in the protein now faces the other side of the plasma membrane.[12] The carrier protein substrate is released at that site, according to its binding affinity there.
Passive transport Facilitated diffusion, also called carrier-mediated osmosis, is the movement of molecules across the cell membrane via special transport proteins that are embedded within the cellular membrane. Large, insoluble molecules, such as glucose, vesicles and proteins require a carrier molecule to move through the plasma membrane.[2] Therefore, it will bind with its specific carrier proteins, and the complex will then be bonded to a receptor site and moved through the cellular membrane. Facilitated diffusion is a passive process: the solutes move down their concentration gradient and do not require the expenditure of cellular energy for this process. Carrier proteins and channel proteins allow for the diffusion of molecules across the cell membrane. Carrier proteins undergo conformational alterations to allow molecules to pass, while channel proteins form unblocked pores.[3]
Cell membrane Cell membranes contain a variety of biological molecules, notably lipids and proteins. Composition is not set, but constantly changing for fluidity and changes in the environment, even fluctuating during different stages of cell development. Specifically, the amount of cholesterol in human primary neuron cell membrane changes, and this change in composition affects fluidity throughout development stages.[19]