when constructing an dna molecule what is important when determining the orientation of each strand
Restriction map If you know of a restriction site placed towards one end of the insert you can determine the orientation by observing the size of the fragments in the gel. Often the orientation of inserts is important and this technique is used to screen for the correct orientation.
Heredity Heritable traits are known to be passed from one generation to the next via DNA, a molecule that encodes genetic information.[2] DNA is a long polymer that incorporates four types of bases, which are interchangeable. The sequence of bases along a particular DNA molecule specifies the genetic information: this is comparable to a sequence of letters spelling out a passage of text.[7] Before a cell divides through mitosis, the DNA is copied, so that each of the resulting two cells will inherit the DNA sequence. A portion of a DNA molecule that specifies a single functional unit is called a gene; different genes have different sequences of bases. Within cells, the long strands of DNA form condensed structures called chromosomes. Organisms inherit genetic material from their parents in the form of homologous chromosomes, containing a unique combination of DNA sequences that code for genes. The specific location of a DNA sequence within a chromosome is known as a locus. If the DNA sequence at a particular locus varies between individuals, the different forms of this sequence are called alleles. DNA sequences can change through mutations, producing new alleles. If a mutation occurs within a gene, the new allele may affect the trait that the gene controls, altering the phenotype of the organism.[8]
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Synapsis This is not to be confused with mitosis. Mitosis also has prophase, but does not ordinarily do pairing of two homologous chromosomes.[3]