why can you not use a glass cuvette for absorbance measurements in the uv spectral range
Cuvette Traditional ultraviolet–visible spectroscopy or fluorescence spectroscopy typically use samples that are liquid - either a liquid substance or a substance dissolved in a solvent. The liquid sample is placed in a cuvette, and the cuvette is then placed in a spectrophotometer for testing. The cuvette can be made of any material that is transparent in the range of wavelengths being tested. Historically, reusable quartz cuvettes were required for measurements in the ultraviolet range, since glass and most plastic absorb light in this wavelength range, creating interference. Now, disposable plastic cuvettes manufactured using specialized ultraviolet-transparent plastics are available. Glass, plastic (traditional or ultraviolet-transparent), and quartz cuvettes are all suitable for measurements made at higher wavelengths (e.g. in the visible spectrum).
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