Availability of food influenced the breeding preferences of the fish. Competition for a suitable mate influenced the breeding preferences. Predators in the pond influenced the breeding preferences of the fish. Speciation due to reproductive isolation influenced the breeding preferences.
I am hesitating between 4 options to answer the following question, which option should I choose?
Question: Mosquito fish found on the islands of the Bahamas live in various isolated freshwater ponds that were once a single body of water. When several male and female mosquito fish are taken from two isolated ponds and placed into a single pond, the breeding preference of each mosquito fish is for fish from its own original pond. Which of these most likely resulted in this breeding preference?
Possibilities:
- Availability of food influenced the breeding preferences of the fish.
- Competition for a suitable mate influenced the breeding preferences.
- Predators in the pond influenced the breeding preferences of the fish.
- Speciation due to reproductive isolation influenced the breeding preferences.

Speciation due to reproductive isolation influenced the breeding preferences.