during 20002010 in chobe district , botswana , we performed 38 necropsies on macroscopically tb - positive mongooses , of which 18 were further evaluated and tb was confirmed by histopathologic examination .
an in - depth histologic evaluation was performed on a subsample of 8 of these animals from the 2008 outbreak .
the most striking feature identified in the sick mongooses was anorexia , followed by nasal distortion and , less commonly , erosions of the nasal planum with involvement of the hard palate . for 7 of the 8 tb - positive animals examined intensively ,
histologic examination detected unequivocal tb lesions in the skin of the nose and the anterior nasal mucosa .
our findings suggested entry of the organism through erosions on the nasal planum , perhaps in association with abrasions , which might occur during foraging .
such lesions were present in the hairless parts of the nose tip of most tb - infected mongooses . furthermore , granulomatous inflammation and mycobacterial organisms were found in the dermis of the skin directly below these erosions .
inflammation and organisms were present in some cases in the nasal mucosa , but erosion was not found .
this finding is consistent with pneumonic tb being present in only a few advanced cases of disseminated tb .
histologically , the tb pneumonia was determined to be hematogenous rather than bronchogenous ( i.e. , by inhalation ) ; thus , no evidence for aerosol transmission was found .
rather , pathogen invasion appears to have occurred through the nasal planum of the mongoose , and hematogenous or lymphatic spread through the body was a strikingly unique feature of this particular m. tuberculosis complex organism .
samples from histologically positive mongooses were positive by pcr for the mpb70 target , is6110 element , and 16s rdna , indicating that the infective organism was a member of the m. tuberculosis complex ( 3,4 ) .
samples were further evaluated with an m. tuberculosis complex  specific multiplex pcr ( 5 ) , which provided distinct results , differing clearly from those for other members of the m. tuberculosis complex ( table 1 ) . * determined by pcr amplification ( 5 ) .
rd , region of difference ; rd1 , m. bovis bcg  specific rd1 ; rd1 , m. microti  specific rd1 ; rd2 , m. pinnipedii  specific rd2 ; p , present ; a , absent . the gyrb gene ( encoding for gyrase b ) sequence , used to identify m. tuberculosis complex member  specific sequence single - nucleotide polymorphisms ( snps ) ( 6 ) , identified the position of the organism as being situated between dassie bacillus and m. africanum subtype 1(a ) and showed no detectable new snps ( figure 2 , panel a ) .
amplification of rd701 and rd702 and lack of snps in rpob and hsp65 genes demonstrated that this organism was not a member of the m. africanum subtype 1(a ) sublineage ( 6,11 ) ( figure 2 , panel a ) .
a ) schematic of the phylogenetic relationships among mycobacterium tuberculosis complex species , including newly discovered m. mungi , based on the presence or absence of regions of difference ( gray boxes ) as well as specific single - nucleotide polymorphisms ( white boxes ) , modified from ( 7 ) .
b ) spoligotype of m. mungi compared with representative spoligotypes from other m. tuberculosis complex species ( 810 ) .
three markers were evaluated to definitively exclude the organism from being dassie bacillus : n - rd25 deletion , rd1 deletion , and snp 389 in the gene rv0911 ( 6 ) .
the n - rd25 amplification gave the right product for the presence of a deletion in this region , and further sequencing confirmed that it contained a deletion in the same position as n - rd25 ; however , sequencing of rv0911 showed no snp at position 389 , indicating that this organism was not dassie bacillus .
as a final test , we amplified the rd1 region but were unable to amplify a product from the mongoose isolates .
we redesigned primers to amplify a smaller region of different diagnostic sizes ( 248 bp when rd1 is deleted and 318 bp when rd1 is intact ) but still had no amplification .
this finding indicates that the rd1 region is deleted in this organism but that the deletion is larger than that of the dassie bacillus .
we then used spoligotyping analysis ( 12 ) to further evaluate mongoose samples and identified a unique spoligotype pattern with no known matches in the international spoligotyping database spoldb4 ( 9 ) or the m. bovis 
this pattern was constant during 20002009 in different mongoose troops and locations ( table a1 ) .
this unique spoligotyping pattern will enable identification of m. mungi in future tb surveillance programs .
for these same isolates , the full set of 24 mycobacterial interspersed repetitive unit  variable number tandem repeats ( 13 ) identified a pattern that was unique compared with others in the international database at www.miru-vntrplus.org ( table 2 ) .
evidence of multiple m. mungi substrains circulating between years and within social groups ( 6601b and 6600b ) in the same outbreak year ( table 2 ) suggests complexity in m. mungi transmission and potential evolution of the organism over the past decade . * miru , mycobacterial interspersed repetitive unit ; vntr , variable number tandem repeats ; db , dassie bacillus ; m. a. , m. africanum ; ob , oryx bacillus ; m. p. , 
 m. pinnipedii ; m. t. , 
 m. tuberculosis ;  , not applicable .
gray shading highlights differences between the mycobacterium tuberculosis complex species and m. mungi as well as variation within m. mungi samples evaluated .
m. africanum west african 2 strain 8163/02 ( st181 ) , m. microti strain 287/99 ( st539 ) , m. pinnipedii strain 7739/01 , m. caprae strain 5358/99 ( st647 ) , and m. bovis strain 8490/00 ( st482 ) data from www.miru-venterplus.org .
this newly identified mycobacterial pathogen has many unique ecologic characteristics that set it apart from other members of the m. tuberculosis complex .
first , it causes high numbers of deaths of banded mongooses , threatening local extinction of smaller social groups .
second , rather than having a primary respiratory transmission route with direct transmission between individuals , as is characteristic of other m. tuberculosis complex species , m. mungi appears to infect banded mongooses by means of a nonrespiratory route through the nasal planum , suggestive of environmental transmission .
third , the time from clinical presentation to death for affected mongooses is generally short ( 23 months ) compared with that for other m. tuberculosis complex pathogens ( more chronic infection , can take years to progress to death )
. acute illness and high mortality rates , as seen in banded mongooses with m. mungi infection , have been associated with extremely isolated human communities newly exposed to tb ( 14 ) .
conventional laboratory culture , biochemical testing , and a limited molecular evaluation were insufficient for differentiating m. mungi from m. tuberculosis ( 2 ) .
organism differentiation required an extensive suite of additional molecular assessments not available at that time , thus underscoring the difficulty of diagnosing m. tuberculosis complex agents correctly and the inability of most national health laboratories to do so .
the fact that new host - adapted m. tuberculosis complex species continue to be identified illustrates the diversity within the m. tuberculosis complex and stresses the need for sensitive techniques for species differentiation .
the identification of this previously unknown pathogen within the m. tuberculosis complex identifies new concerns for human and animal health and illustrates the continuing scope of the threat posed by tb pathogens .
 seven outbreaks involving increasing numbers of banded mongoose troops and high death rates have been documented . 
 we identified a mycobacterium tuberculosis complex pathogen , m. mungi sp . 
 nov . 
 , as the causative agent among banded mongooses that live near humans in chobe district , botswana . 
 host spectrum and transmission dynamics remain unknown . 