the subject population consisted of five male and three female individuals of scandinavian origin , chosen from an orthodontic clinic for the study .
all were 10 to 14 years old and had premolars scheduled for extraction due to orthodontic reasons .
a total of 23 premolars were used and the premolars had no signs of clinical wsls at baseline .
for all scheduled study visits , participants did not brush their teeth the morning prior to sampling and had no breakfast within an hour before sampling .
all appointments were scheduled between 9 am and 12 pm . while enrolled in the study , participants brushed their teeth with the non - fluoride toothpaste solidox ( lilleborg as , oslo , norway ) and did not use any fluoride or antimicrobials , such as chlorhexidine or triclosane .
the study was approved by the regional ethics committee ( rek sr - st , pb 1130 , blindern , oslo , norway ) .
the subjects and their guardians signed an informed consent form before the start of the study .
orthodontic bands with two metal posts ( 0.8 mm thick ) welded onto the inner buccal surface specifically designed for plaque accumulation ( 16 ) , were banded on premolars destined for extraction during the first visit ( fig .
temp bond ne ( kerr corporation , orange , ca , usa ) was used to cement the bands in place ( 17 ) .
plaque samples were taken from premolars at baseline ( immediately before banding ) and at the final visit 7 weeks after banding .
a lip spreader was used to isolate the premolars and the buccal surfaces were allowed to air dry before sample collection .
the same examiner collected all samples with a sterile orthodontic wire ( fig . 1 ) and immediately suspended each sample in 300 l te buffer ( 10 mm tris 
the samples were transported to the laboratory using the nalgenetm labtop cooler ( 20c ) , before placing them at 80c until further processing . for baseline sampling , the buccal enamel surface where the band would be placed was sampled with a probe . at the final visit ,
the probe was inserted into the space between the band and the buccal tooth surface and the plaque sample was collected from the enamel surface . after collecting the sample at the final visit , the examiner extracted the premolars and the orthodontic treatment was continued as planned by the orthodontist .
the buccal surface of the extracted premolars was carefully cleaned with water , air dried and then the buccal lesion was given a score in the following manner ( fig .
2 ) : no wsls=1 , minor wsls=2 and severe wsls=3 ( 18 ) . resulting white spot lesion after 7 weeks of plaque retention .
dna was isolated from clinical samples using a ready - lyse lysozyme solution ( epicentre ) for overnight incubation before using a masterpure dna purification kit ( epicentre ) according to the manufacturer 's directions .
purified dna samples were analyzed using homim , which was developed to simultaneously detect 300 of the most prevalent oral bacterial species .
briefly , 16s rrna - based , reverse - capture oligonucleotide probes ( typically 1820 bases ) were printed on aldehyde - coated glass slides .
16s rrna genes were pcr amplified from dna extracts using 16s rrna universal forward and reverse primers and labeled via incorporation of cy3-dctp in a second nested pcr .
after washing , the microarray slides were scanned using an axon 4000b scanner and crude data were extracted using genepix pro software .
microbial community profiles were generated from image files of scanned homim arrays using a homim online analysis tool ( available at : http://bioinformatics.forsyth.org/homim/ ) .
the detection of a particular taxon in a sample was determined by the presence of a fluorescent spot for that unique probe .
a mean intensity for each taxon was calculated from the hybridization spots of the same probe and the signals were normalized and calculated as previously described ( 15 ) .
the range of signal levels was obtained by raising normalized signal intensities to the power of 0.3 .
any original signal that was less than two times the background value was reset to 1 and was assigned to the signal level 0 ; indicating absence of a particular taxon in a sample .
the remaining signals , those greater than 1 , were categorized into scores from 1 to 5 ; corresponding to different signal levels . to determine how bacterial community composition varied across samples ,
we compared total hybridization profiles obtained by homim arrays for each sample using correspondence analysis ( coa ) in mev 4.6 ( 19 ) . analysis was performed on the absolute intensity of homim data ( frequency of scores from 0 to 5 ) as well as binary data ( presence / absence ) .
wilcoxon rank sum test and t - test were used to identify statistically significant differences between groups ( baseline and final visit ; wsls 1 , 2 and 3 ; and plaque indices 0 , 1 and 2 )
. a p - value of < 0.05 was considered significant . false discovery rate ( fdr ) using benjamini - hochberg correction was used to control for multiple hypothesis . for the cluster analysis ,
jmp 9.0 ( www.jmp.com/ ) and r statistical package ( www.r-project.org/ ) were used in statistical analyses .
the subject population consisted of five male and three female individuals of scandinavian origin , chosen from an orthodontic clinic for the study .
all were 10 to 14 years old and had premolars scheduled for extraction due to orthodontic reasons .
a total of 23 premolars were used and the premolars had no signs of clinical wsls at baseline .
for all scheduled study visits , participants did not brush their teeth the morning prior to sampling and had no breakfast within an hour before sampling .
all appointments were scheduled between 9 am and 12 pm . while enrolled in the study , participants brushed their teeth with the non - fluoride toothpaste solidox ( lilleborg as , oslo , norway ) and did not use any fluoride or antimicrobials , such as chlorhexidine or triclosane .
the study was approved by the regional ethics committee ( rek sr - st , pb 1130 , blindern , oslo , norway ) .
the subjects and their guardians signed an informed consent form before the start of the study .
orthodontic bands with two metal posts ( 0.8 mm thick ) welded onto the inner buccal surface specifically designed for plaque accumulation ( 16 ) , were banded on premolars destined for extraction during the first visit ( fig .
temp bond ne ( kerr corporation , orange , ca , usa ) was used to cement the bands in place ( 17 ) .
plaque samples were taken from premolars at baseline ( immediately before banding ) and at the final visit 7 weeks after banding .
a lip spreader was used to isolate the premolars and the buccal surfaces were allowed to air dry before sample collection .
the same examiner collected all samples with a sterile orthodontic wire ( fig . 1 ) and immediately suspended each sample in 300 l te buffer ( 10 mm tris 
the samples were transported to the laboratory using the nalgenetm labtop cooler ( 20c ) , before placing them at 80c until further processing . for baseline sampling , the buccal enamel surface where the band would be placed was sampled with a probe . at the final visit ,
the probe was inserted into the space between the band and the buccal tooth surface and the plaque sample was collected from the enamel surface . after collecting the sample at the final visit , the examiner extracted the premolars and the orthodontic treatment was continued as planned by the orthodontist .
the buccal surface of the extracted premolars was carefully cleaned with water , air dried and then the buccal lesion was given a score in the following manner ( fig .
2 ) : no wsls=1 , minor wsls=2 and severe wsls=3 ( 18 ) . resulting white spot lesion after 7 weeks of plaque retention .
dna was isolated from clinical samples using a ready - lyse lysozyme solution ( epicentre ) for overnight incubation before using a masterpure dna purification kit ( epicentre ) according to the manufacturer 's directions .
purified dna samples were analyzed using homim , which was developed to simultaneously detect 300 of the most prevalent oral bacterial species .
briefly , 16s rrna - based , reverse - capture oligonucleotide probes ( typically 1820 bases ) were printed on aldehyde - coated glass slides .
16s rrna genes were pcr amplified from dna extracts using 16s rrna universal forward and reverse primers and labeled via incorporation of cy3-dctp in a second nested pcr .
after washing , the microarray slides were scanned using an axon 4000b scanner and crude data were extracted using genepix pro software .
microbial community profiles were generated from image files of scanned homim arrays using a homim online analysis tool ( available at : http://bioinformatics.forsyth.org/homim/ ) .
the detection of a particular taxon in a sample was determined by the presence of a fluorescent spot for that unique probe .
a mean intensity for each taxon was calculated from the hybridization spots of the same probe and the signals were normalized and calculated as previously described ( 15 ) .
the range of signal levels was obtained by raising normalized signal intensities to the power of 0.3 . any original signal that was less than two times the background value was reset to 1 and
was assigned to the signal level 0 ; indicating absence of a particular taxon in a sample .
the remaining signals , those greater than 1 , were categorized into scores from 1 to 5 ; corresponding to different signal levels . to determine how bacterial community composition varied across samples , we compared total hybridization profiles obtained by homim arrays for each sample using correspondence analysis ( coa ) in mev 4.6 ( 19 ) .
analysis was performed on the absolute intensity of homim data ( frequency of scores from 0 to 5 ) as well as binary data ( presence / absence ) .
wilcoxon rank sum test and t - test were used to identify statistically significant differences between groups ( baseline and final visit ; wsls 1 , 2 and 3 ; and plaque indices 0 , 1 and 2 ) .
a p - value of < 0.05 was considered significant . false discovery rate ( fdr ) using benjamini - hochberg correction was used to control for multiple hypothesis . for the cluster analysis ,
jmp 9.0 ( www.jmp.com/ ) and r statistical package ( www.r-project.org/ ) were used in statistical analyses .
at the end of seven weeks at the final visit , 75% of the premolars had developed wsls on their buccal surfaces underneath the retention band ( fig .
2 ) . there was a clear shift of the microbiota from plaque found on sound enamel ( baseline ) as compared to plaque retained after seven weeks ( final visit ) .
microbial communities at baseline were found to be significantly different ( p<0.001 ; t test ) from the microbial communities at the final visit ( figs . 3 and 4 ) .
signature for each individual ; samples from the same individual , in general , were more similar to one another than when taken from two different individuals ( fig .
plot of correspondence analysis ( ca ) of the enamel bacterial community using binary data ( presence / absence ) .
only communities with data for both the baseline and final visit are plotted and labeled by color according to visit .
the baseline samples are labeled with solid circles ; samples from the final visit ( 7 weeks later ) are labeled with open circles .
microbial communities from the baseline visit are significantly different ( p<0.001 ; t test ) from the final visit .
mean frequencies of bacterial probes that were significantly different ( p<0.05 ; wilcoxon test ) among subjects at baseline and final visit .
* indicates adjusted p ( benjamini / hochberg ) values p<0.01 and * * p<0.001 .
fourteen bacterial taxa were found to be prevalent in plaque covering sound enamel at baseline ( p<0.01 ; fig .
the most commonly detected species in greater than 80% of samples were gemella morbillorum , kingella 
 oralis and capnocytophaga 
 granulosa .
all but campylobacter concisus and campylobacter showae are capable of fermenting carbohydrates to acid , although c. concisus and c. showae can produce acetate and or succinate with formate and fumarate in the culture medium ( 20 ) .
twenty - five bacterial species were significantly increased in plaque behind the retaining bands after seven weeks ( fig .
4 ) . many of these species have been previously associated with caries development , such as streptococcus 
 mutans , other species of streptococcus and species of lactobacillus .
other species less known to be associated with caries development included atopobium 
 parvulum , dialister 
 invisus , and species of prevotella and scardovia . of note , 25% of teeth did not develop a lesion after seven weeks .
consequently , we compared the changes in microbiota on pairs of samples between baseline and for those samples that did not develop a lesion .
for this small sample size , there were no taxa that were significantly more prevalent at baseline or at the final visit .
the average number of taxa for plaque index 0 , 1 and 2 was 38.50 , 44.13 and 53.68 , respectively .
the wilcoxon signed - rank test was used for each pair ; plaque indices 0 and 2 were compared : p - value=0.0014 , and plaque indices 1 and 2 : p - value=0.0247 .
there were differences in the prevalence of specific taxa in supragingival plaque taken from wsl 1 sites as compared to supragingival plaque from wsl 3 sites at the final visit .
ot136/ot148 , megasphaera micronuciformis , and selenomonas sputigena , were more prevalent in wsl 1 samples ( no lesions ) , ( p<0.01 ) .
however , due to the low number of samples ( n ) , the results were not significant ( e.g. p>0.05 ) when adjusted for multiple comparisons .
at the end of seven weeks at the final visit , 75% of the premolars had developed wsls on their buccal surfaces underneath the retention band ( fig .
2 ) . there was a clear shift of the microbiota from plaque found on sound enamel ( baseline ) as compared to plaque retained after seven weeks ( final visit ) .
microbial communities at baseline were found to be significantly different ( p<0.001 ; t test ) from the microbial communities at the final visit ( figs . 3 and 4 ) .
signature for each individual ; samples from the same individual , in general , were more similar to one another than when taken from two different individuals ( fig .
plot of correspondence analysis ( ca ) of the enamel bacterial community using binary data ( presence / absence ) .
only communities with data for both the baseline and final visit are plotted and labeled by color according to visit .
the baseline samples are labeled with solid circles ; samples from the final visit ( 7 weeks later ) are labeled with open circles .
microbial communities from the baseline visit are significantly different ( p<0.001 ; t test ) from the final visit .
mean frequencies of bacterial probes that were significantly different ( p<0.05 ; wilcoxon test ) among subjects at baseline and final visit .
* indicates adjusted p ( benjamini / hochberg ) values p<0.01 and * * p<0.001 .
fourteen bacterial taxa were found to be prevalent in plaque covering sound enamel at baseline ( p<0.01 ; fig .
the most commonly detected species in greater than 80% of samples were gemella morbillorum , kingella 
 oralis and capnocytophaga 
 granulosa .
all but campylobacter concisus and campylobacter showae are capable of fermenting carbohydrates to acid , although c. concisus and c. showae can produce acetate and or succinate with formate and fumarate in the culture medium ( 20 ) .
twenty - five bacterial species were significantly increased in plaque behind the retaining bands after seven weeks ( fig .
4 ) . many of these species have been previously associated with caries development , such as streptococcus 
 mutans , other species of streptococcus and species of lactobacillus .
other species less known to be associated with caries development included atopobium 
 parvulum , dialister 
 invisus , and species of prevotella and scardovia . of note , 25% of teeth did not develop a lesion after seven weeks .
consequently , we compared the changes in microbiota on pairs of samples between baseline and for those samples that did not develop a lesion .
for this small sample size , there were no taxa that were significantly more prevalent at baseline or at the final visit .
the average number of taxa for plaque index 0 , 1 and 2 was 38.50 , 44.13 and 53.68 , respectively .
the wilcoxon signed - rank test was used for each pair ; plaque indices 0 and 2 were compared : p - value=0.0014 , and plaque indices 1 and 2 : p - value=0.0247 .
there were differences in the prevalence of specific taxa in supragingival plaque taken from wsl 1 sites as compared to supragingival plaque from wsl 3 sites at the final visit .
ot136/ot148 , megasphaera micronuciformis , and selenomonas sputigena , were more prevalent in wsl 1 samples ( no lesions ) , ( p<0.01 ) .
however , due to the low number of samples ( n ) , the results were not significant ( e.g. p>0.05 ) when adjusted for multiple comparisons .
the results clearly showed an ecological shift in dental plaque over developing wsls in enamel .
many bacterial species , including typical caries - associated species such as s. mutans and lactobacillus spp . , increased with the development of wsls .
consequently , the bacterial community composition associated with the progression of wsl is specific and much more complex than previously believed and should be explored in future studies .
our results not only confirmed previous studies ( 6 , 8 , 10 , 11 ) , but additional species or phylotypes that were significantly associated with the longitudinal progression of caries in the secondary dentition were identified ( table 1 ) ( 10 ) . 
 
comparison of statistically significant bacterial taxa associated with carious lesions in the secondary dentition many of the bacterial species found to be increased in plaque covering sound enamel and in plaque behind the plaque retaining bands are capable of producing acid .
bacteria capable of producing caries need to be both acidogenic ( able to produce acid ) and aciduric ( able to survive in an acid environment ) ( 21 ) .
it is noteworthy that non - mutans streptococci , such as those found in wsls , are capable of acidogenesis at low ph and typically outnumber mutans streptococci .
consequently , several species of non - mutans streptococcus have been implicated in caries production ( 13 ) .
indeed , in this study several species or phylotypes were significantly more prevalent in wsls ( fig .
our results provided strong evidence in support of the ecological plaque hypothesis , that is , enamel caries develops due to changes in local environmental conditions ( plaque retaining bands ) , which disrupt the natural balance between plaque and the host , leading to enrichment of organisms that can potentially cause caries .
in addition , our results showed that many bacterial species other than s. mutans and lactobacilli are associated with caries in vivo . in summary ,
the bacterial diversity of the predominant oral microbiota associated with development of cariogenic lesions was determined using homim in an in vivo model .
our results indicate that the microbiota on intact enamel was significantly different from that of the microbiota associated with wsls developed for seven weeks under protected metal bands .
the microbial communities in dental plaque associated with caries included species of the genera acidaminococcaceae , streptococcus , lactobacillus , veillonella , prevotella , solobacterium , scardovia , and atopobium .
some of these were associated with the increasing severity of wsls such as s. wiggsiae , s. salivarius and v. atypica and might play important roles in the process of caries development .
the in vivo model of microbial community succession provided novel insights into the microbial community shifts associated with the development of wsls , and likely dental caries .
consequently , this model can be used to study the efficacy of treatment or perturbations , such as diet , antibiotics , and other prophylactic and restorative treatments , and may help design more effective interventions and preventions .
there is no conflict of interest in the present study for any of the authors .
 backgrounddental caries is one of the most common diseases in the world . 
 however , our understanding of how the microbial community composition changes in vivo as caries develops is lacking.objectivean in vivo model was used in a longitudinal cohort study to investigate shifts in the microbial community composition associated with the development of enamel caries.designwhite spot lesions were generated in vivo on human teeth predetermined to be extracted for orthodontic reasons . 
 the bacterial microbiota on sound enamel and on developing carious lesions were identified using the human oral microbe identification microarray ( homim ) , which permits the detection of about 300 of the approximate 600 predominant bacterial species in the oral cavity.resultsafter only seven weeks , 75% of targeted teeth developed white spot lesions ( 8 individuals , 16 teeth ) . 
 the microbial community composition of the plaque over white spot lesions differed significantly as compared to sound enamel . 
 twenty - five bacterial taxa , including streptococcus 
 mutans , atopobium 
 parvulum , dialister 
 invisus , and species of prevotella and scardovia , were significantly associated with initial enamel lesions . in contrast , 14 bacterial taxa , including species of fusobacterium , campylobacter , kingella , and capnocytophaga , were significantly associated with sound enamel.conclusionsthe bacterial community composition associated with the progression of enamel lesions is specific and much more complex than previously believed . 
 this investigation represents one of the first longitudinally - derived studies for caries progression and supports microbial data from previous cross - sectional studies on the development of the disease . 
 thus , the in vivo experiments of generating lesions on teeth destined for extraction in conjunction with homim analyses represent a valid model to study succession of supragingival microbial communities associated with caries development and to study efficacy of prophylactic and restorative treatments . 