( baill . ) is a source plant of traditional chinese medicine . the s. chinensis fruit ( sf ) , which has five - flavored fruits ( salty , pungent , bitter , sweet and sour ) , is called  wuweizi  in china .
it naturally distribute in china , russian , korea and japan   and was used in treatment of diseases including liver injury , tumor inhibition , urinary tract disorders , insomnia and palpitation , even inhibit both hiv-1 rt - associated dna polymerase activity and virus replication .
in addition to its application as a chinese herbal medicine . it is also used for making beverages , tea , wine , health food industries and cosmetics .
therefore , recent research has been focused on extracting active ingredients and identifying function . despite of the importance of s. chinensis ,
the next generation sequencing technology has dramatically improved the efficiency and speed of gene discovery .
two cultivars  yanhong ( red skin ) and  jinwuwei ( white skin ) were grown in orchard located in zuojia town , jilin city , jilin province , china ( 440647 n 1260718 e ) that belong to the plant resources nursery of institute of special wild economic animal and plant science , chinese academy of agricultural sciences .
fours samples , including fruits and skins , were harvested and immediately frozen in liquid nitrogen for further experiments .
total rna was extracted using modified ctab method , library construction and high - throughput sequencing for each sample was performed at a contract sequencing company ( novogene , china ) .
the cdna library was sequenced using an illumina hiseq2500 platform . with the purpose of determining the red fruit , white fruit , red skin of red fruit and white skin of white fruit of s. chinensis .
we obtained a total of 72.5 million , 55.1 million , 68 million and 69.3 million raw data reads from four sequencing libraries prepared , respectively ( table 1 ) , >  94.9% bases has a q value   20 ( an error probability of 0.035% ) . after cleaning and quality checks , the de novo assembly of all sequencing data using the trinity method .
it generated 92,415 all - transcripts with an average length of 496  bp and an n50 of 1466  bp ; and 71,443 all - unigenes were achieved .
of these , 46,461 ( 65.0% ) were 200500  bp , 11,322 were 5001000  bp , 8612 were 12  kb and the remaining 5078 were >  2  kb ( table 2 ) .
the results provides us useful information for further explorer the gene synthesis pathways of active ingredients .
two cultivars  yanhong ( red skin ) and  jinwuwei ( white skin ) were grown in orchard located in zuojia town , jilin city , jilin province , china ( 440647 n 1260718 e ) that belong to the plant resources nursery of institute of special wild economic animal and plant science , chinese academy of agricultural sciences .
fours samples , including fruits and skins , were harvested and immediately frozen in liquid nitrogen for further experiments .
total rna was extracted using modified ctab method , library construction and high - throughput sequencing for each sample was performed at a contract sequencing company ( novogene , china ) .
with the purpose of determining the red fruit , white fruit , red skin of red fruit and white skin of white fruit of s. chinensis .
we obtained a total of 72.5 million , 55.1 million , 68 million and 69.3 million raw data reads from four sequencing libraries prepared , respectively ( table 1 ) , >  94.9% bases has a q value   20 ( an error probability of 0.035% ) . after cleaning and quality checks , the de novo assembly of all sequencing data using the trinity method .
it generated 92,415 all - transcripts with an average length of 496  bp and an n50 of 1466  bp ; and 71,443 all - unigenes were achieved .
of these , 46,461 ( 65.0% ) were 200500  bp , 11,322 were 5001000  bp , 8612 were 12  kb and the remaining 5078 were >  2  kb ( table 2 ) .
the results provides us useful information for further explorer the gene synthesis pathways of active ingredients .
 the fruit of schisandra chinensis turcz . 
 ( baill . ) , namely  wuweizi  in china , is a well - known herbal medicine and health food . at present , research focused on the extraction of effective chemical component and function identification . 
 little known about the secondary metabolism gene pathway of chemical composition . 
 its fruit color usually red , however , the white fruit color variation has been found . 
 it made us interested in exploring which gene change lead to this result . in order to understand the genetic background of s. chinensis 
 , we performed a transcriptome analysis of s. chinensis , including red fruit and skin of  
 yanhong cultivar and white fruit and skin of  
 jinwuwei. 
 we obtained 26.4  gb raw data ( ncbi accession number : ssr4449123 ) . 
 de novo transcriptome assembly using trinity revealed 92,415 transcripts and generated 71,443 unigenes . 
 all unigenes were annotated in database.this study provides transcriptome of s. chinensis , which might be useful for comparative transcriptome analyses and understand gene expression of secondary metabolites . 