since the beginning of mankind nature has been contributing considerably to drug discovery for human beings by providing remedial treatments .
one of nature 's treasures is the marine biotope , which occupies almost three quarters of the earth 's surface ( fenical , 1993 ; whitehead , 1999 ) .
marine natural products play an increasingly important role in biomedical research and drug development , either directly as drugs or as lead structures for bioinspired chemical drug synthesis ( molinski et  al . ,
many marine natural products , especially those isolated from macroorganisms , have already undergone clinical trials ( newman and cragg , 2004 ) . during the last decades , however , repeated isolation of known metabolites and a reduced hitrate of novel compounds from marine macroorganisms were observed .
hence , natural product chemists are turning their interest to so far less investigated drug sources , such as marine fungi and bacteria , which turned out to be a vast untapped reservoir of metabolic diversity .
thus , research on chemistry of natural products derived from marine microorganisms has increased tremendously in recent years due to the demand for compounds having potential pharmaceutical applications or economical value as cosmetics , drugs , fine chemicals and functional personalcare products ( andersen and williams , 2000 ) .
in contrast to macroorganisms , microorganisms represent promising natural product sources having the advantage of feasible and sustainable production of large quantities of secondary metabolites with reasonable cost , by largescale cultivation and fermentation of the source organisms ( waites et  al . , 2001 ) . furthermore , to adapt and survive in the marine ecosystem , characterized by very special conditions that differ from those found in other habitats , marine microorganisms sometimes accumulate structurally unique bioactive secondary metabolites not found in terrestrial organisms ( bhakuni and rawat , 2005 ) .
natural products research still has an enormous unexploited potential , and the significant advantages and disadvantages of natural productderived molecules as drug candidates for development have been highlighted in numerous articles ( rogers , 2004 ) .
the importance of natural product discovery from microorganisms started only after the largescale production of penicillin ( 1 ) during world war ii ( numbers 1101 in bold refer to chemical structures 1101 at the end of this article ) .
after the end of the war , pharmaceutical companies refocused on the search for new bioactive biomolecules . in the 1970s for example , cholesterol biosynthesis inhibitors , compactin ( 2 ) ( larsen et  al . , 2007 ) and mevinolin ( 3 ) ( araki and konoike , 1997 ) were discovered .
the discovery of compactin and mevinolin enabled the development of the highly successful statin therapeutics ( 4 ) ( endo , 1992 ) , which even today are considered as block buster in pharmaceutical sales . the discovery of streptomycin , gentamicin , omegamycin ( 5 ) and other antibiotics pushed the pharmaceutical industry to implement large research and developing programs based on natural product discovery , with a recent emphasis on marine microbial fermentation based technologies .
the water column of the oceans contains approximately 10 bacterial cells per millilitre ( hagstrm et  al . , 2002 ) .
marine bacteria and fungi are of great interest as novel and rich sources of biologically active products .
they live in close association with softbodied marine organisms , which lack obvious structural defence mechanisms , and thus rely on chemical defence by production of bioactive secondary metabolites , either by themselves or by associated microflora , to survive in their extreme habitat ( jensen and fenical , 1994 ) . in the last decades ,
the number of reported secondary metabolites from marine bacteria and fungi has steadily increased ( fenical , 1993 ; kobayashi and ishibashi , 1993 ; bernan et  al . , 1997 ; faulkner , 1997 ; 1998 ; 1999 ; 2000 ; 2001 ; blunt et  al .
, 2003 ; 2004 ; 2005 ; 2006 ; 2007 ; 2008 ; 2009 ; hill , 2003 ) , thus reflecting the growing attention by groups from academia and industry . in the year 2007 alone
961 new compounds were described from marine microorganisms reflecting an increase of 24% compared with the number of compounds reported for 2006 ( blunt et  al . , 2009 ) . in the following , selected examples of new secondary metabolites from bacteria and fungi , published in the period 200709 , that live in association with marine macroorganisms , such as sponges , algae and mangrove plants , are presented , with special emphasis on bioactive products and their modes of their action , as well as source organisms and place of origin .
chemical structures are only shown for new compounds , or for previously reported compounds with newly reported biological activities .
since the discovery of penicillin in 1928 ( fleming , 1929 ) , intensive studies , mainly on soilderived bacteria and fungi , demonstrated that microorganisms are a rich source of structurally unique bioactive substances ( fenical , 1993 ) .
the increasing need for new antimicrobial agents able to control emerging diseases or resistant strains of microorganisms inspired a growing number of research groups to explore the oceans for new bioactive compounds . throughout the years
, extensive screening programs were developed worldwide and great efforts have been devoted aiming of the isolation of new metabolites from marine microorganisms .
cultures of the marine bacterial isolate brevibacillus laterosporus png276 obtained from papua new guinea yielded a new lipopeptide named tauramamide ( 6 ) , together with its methyl ( 7 ) and ethyl esters ( 8) .
structures were elucidated by analysis of nmr and ms data and by chemical degradation as well as by total synthesis of tauramamide ( 6 ) and tauramamide ethyl ester ( 8) .
compounds 6 and 8 showed potent [ minimum inhibitory concentration ( mic ) values of 0.11  m ] and relatively selective activity against the important grampositive human pathogen enterococcus sp . the ethyl ester ( 8) showed weaker activity against multidrugresistant staphylococcus aureus , but neither compound was appreciably active against the yeast c. albicans .
tauramamide ( 6 ) is a new lipopeptide antibiotic that contains two d amino acids and is acylated at the nterminus .
both structural features are hallmarks of nonribosomal peptide synthase biosynthetic origin ( desjardine et  al . , 2007 ) .
a member of the new bacterial genus marinispora ( strain nps008920 ) was isolated from a sediment sample collected in cocos lagoon , guam .
chemical investigation of this strain afforded a series of novel 2alkylidene5alkyl4oxazolidinones , lipoxazolidinone a ( 9 ) , b ( 10 ) and c ( 11 ) .
compounds 911 showed broad spectrum antimicrobial activities similar to those of the commercial antibiotic linezolid ( zyvox ) ( barbachyn and ford , 2003 ) .
lipoxazolidinones a ( 9 ) , b ( 10 ) and c ( 11 ) and the hydrolysis product 12 were screened against a panel of various grampositive and gramnegative bacteria .
compound 9 showed broad spectrum activity , with mic values ranging from 1.56 to 15.57  m against grampositive bacteria and 37.38  m against two strains of haemophilus influenzae .
compounds 10 and 11 showed also broad spectrum antibacterial activity , albeit with lesser overall potency than 9 .
in contrast , the hydrolysis product of 12 showed only weak activity against mssa ( methicillinsensitive s. aureus ) , indicating the importance of an intact oxazolidinone ring system .
while the oxazolidinone heterocycle is a common structural motif shared by the lipoxazolidinones and linezolid , the compounds are clearly distinguished as 4 and 2oxazolidinones , respectively , and each class is uniquely substituted .
thus , the 4oxazolidinones offer a unique scaffold of compounds with antibiotic therapeutic potential ( macherla et  al . , 2007 ) .
the new marine actinomycete , nps12745 , was recently isolated and described from a marine sediment collected off the coast of san diego , california .
the analysis of the fulllength 16s rrna sequence indicated that nps12745 is a novel strain of the recently described marine actinomycete genus marinispora .
chemical investigation of this novel strain yielded a series of new chlorinated bisindole pyrroles , lynamicins ae ( 1317 ) .
the bisindole pyrrole derivatives are small molecules , and include chromopyrrolic acid which was previously isolated from chromobacterium violaceum ( hoshino et  al . , 1993 ) as well as lycogarubins ac known from the myxomycete lycogala epidendrum ( frde et  al . , 1994 ; hashimoto et  al . ,
the antibacterial activity of the compounds against a panel of grampositive and gramnegative bacteria was studied and substances 14 and 15 were found to be active against s. aureus ( mssa , mrsa : methicillin resistant ) , staphylococcus epidermidis and enterococcus faecalis , suggesting potential for treatment of nosocomial infections ( mcarthur et  al . , 2008 ) .
screening of 100 bacteria , which were isolated from intestinal tract of fish that landed on the baluchistan coast that borders the gulf of karachi , pakistan , afforded an isolate of pseudomonas stutzeri ( cmg 1030 ) that showed pronounced inhibitory activity against several pathogenic bacteria , including mrsa strains .
chemical investigation of the ethyl acetate extract yielded a new antibacterial metabolite named zafrin ( 4methyl5,6,7,8tetrahydro1 ( 4h)phenanthrenone ) ( 18 ) .
the mic of zafrin ( 235.85589.62  m ) compared favourably with other novel antimicrobials such as 2,4diacetylphloroglucinol ( 2.384.76  mm ) ( isnansetyo et  al . , 2003 ) .
interestingly , the killing rate of zafrin against bacillus subtilis was faster than for ampicillin , vancomycin or tetracycline .
zafrin does not target the bacterial cell wall and its pattern of lysis resembles that of compounds such as nisin ( 14.91  m ) and triton x100 , which disrupt the cell membrane .
it was suggested that the mode of action of zafrin is via the disruption of the cytoplasmic membranes , since the molecule is amphiphilic ( uzair et  al . , 2008 ) .
alaa 2000 , which was isolated from the marine red alga laurenica spectabilis collected from rasgharib coast of the red sea , egypt , was found to be active against pathogenic microorganisms with mic values ranging from 0.1 to 10  g  ml . chemical and biological screening of the crude extract of this strain afforded the new bioactive compound ayamycin [ 1,1dichloro4ethyl5(4nitrophenyl)hexan2one ] ( 19 ) , which is structurally unique since it contains both chlorine and rarely observed nitro groups , beside being structurally related to compounds such as chrysophanol 8methyl ether ( 20 ) , asphodelin ( 21 ) and justicidin b ( 22 ) .
the isolated compounds were tested for their antimicrobial activities against grampositive and gramnegative bacteria as well as against pathogenic fungi such as candida albicans , aspergillus niger and botrytis fabae .
the most active compound was the new ayamycin ( 19 ) with mic values ranging from 0.31 to 1.57  m ( elgendy et  al . , 2008 ) .
from a marine sediment sample ( collected near la jolla , ca ) at a depth of 51  m , the actinomycete strain cnq418 was isolated .
this strain shared 89.1% 16s rnra gene sequence similarity with its nearest neighbour streptomyces sannurensis .
two prominent products named marinopyrroles a ( 23 ) and b ( 24 ) , were isolated and identified as new secondary metabolites . xray
analysis of marinopyrrole b showed that the natural product exists as an atropoenantiomer with the mconfiguration .
the newly isolated substances 23 and 24 displayed noteworthy activity against methicillinresistant s. aureus , with an mic90 of less than 2  m .
for each of the compounds , cytotoxicity against a human cancer cell line ( hct116 : colon carcinoma ) was less pronounced ( 8.8 and 9.0  m for compounds 23 and 24 respectively ) ( hughes et  al . , 2008 ) .
the marinederived fungus nigrospora sp . was isolated from a sea fan that was collected near similan island , thailand .
when grown in 500  ml erlenmeyer flasks containing potato dextrose broth for 4 weeks , this strain produced four new metabolites named nigrospoxydons a  c ( 2527 ) and nigrosporapyrone ( 28 ) , together with nine known compounds .
the crude ethyl acetate extract obtained from the culture broth showed antibacterial activity against standard s. aureus atcc 25923 ( sa ) and methicillinresistant s. aureus ( mrsa ) , with mic values of 64 and 128  g  ml respectively . only compound 25 and
the known compound ( + ) epoxydon ( 29 ) showed activity against both strains , while the remaining compounds were inactive .
compound 25 was more active than 29 against sa ( mic 152.38  m ) , but was less active against mrsa ( mic  >  304.76  m ) .
compound 29 gave an mic value of 820.51  m against both strains ( trisuwan et  al .
a marine aspergillus species ( family trichocomaceae ) was isolated from the surface of the marine brown alga sargassum horneri collected at gadeok island , busan , korea .
the fungal broth yielded a new polyoxygenated decalin derivative , dehydroxychlorofusarielin b ( 30 ) , which was found to exhibit mild antibacterial activity against s. aureus , methicillinresistant s. aureus , and multidrugresistant s. aureus with mic values of 142.36  m for all strains ( nguyen et  al . , 2007 ) .
fungi of the genus penicillium are known to produce a large variety of compounds with a wide range of biological and pharmacological activities .
the fungal broth yielded two new metabolites , penicipyrone ( 31 ) and penicilactone ( 32 ) , together with three known macrolides ( + ) brefeldin a ( 33 ) ( + ) brefeldin c ( 34 ) and 7oxobrefeldin a ( 35 ) .
compounds 32 , 33 and 35 were tested for antimicrobial activity against methicillinresistant s. aureus sk1 and microsporum gypseum shmu4 .
compound 33 showed the strongest antifungal activity against m. gypseum shmu4 with mic value of 228.57  m , whereas the remaining compounds were inactive ( mic  >  700  m ) . when tested against methicillinresistant s. aureus sk1 all compounds gave mic values of >  700  m ( trisuwan et  al . , 2009 ) .
the culture broth of a marine fungal strain belonging to the genus exophiala ( family herpotrichiellaceae ) afforded the new aspyrone derivatives chlorohydroaspyrones a ( 36 ) and b ( 37 ) .
the fungal strain was isolated from the surface of the marine sponge halichondria panicea collected on bogil island , jeonnam province , korea .
compounds 36 and 37 displayed moderate to weak antibacterial activity when tested against s. aureus , methicillinresistant s. aureus or multidrugresistant s. aureus , showing mic values of 284.09 , 568.18 and 568.18  m , respectively , for compound 36 , as well as 284.09 , 284.09 and 568.18  m , respectively , for compound 37
two new compounds , named xanalteric acids i ( 38 ) and ii ( 39 ) , were isolated from the fungus alternaria sp .
, isolated from fresh healthy leaves of the mangrove plant sonneratia alba ( sonneratiaceae ) , collected in dong zhai gang mangrove garden on hainan island , china .
compounds 38 and 39 exhibited weak antibiotic activity against multidrugresistant s. aureus with mic values of 686.81343.40  m ( kjer et  al . , 2009 ) . the marinederived fungus , ascochyta sp .
ngb4 , was isolated from a floating scrap of festering rope that had been collected at a fishing port in nagasaki prefecture , japan .
chemical investigation of this strain yielded a new spirodioxynaphthalene metabolite , named ascochytatin ( 40 ) .
the relative stereochemistry of the compound was determined by xray crystallographic analysis , and the absolute stereochemistry was identified by the modified mosher 's method . a sensitive screening method for antibacterial agents that inhibit essential encoding genes ( yycg / yycf ) for the bacterial twocomponent regulatory system ( tcs ) , a fundamental system of bacterial response to environmental stress ,
the difference in sensitivity of b. subtilis 168 and cnm2000 towards 40 suggested that 40 inhibited the function of tcs ( yycg / yycf ) in b. subtilis .
compound 40 exhibited relatively strong and specific activity against grampositive bacteria and against the yeast c. albicans .
compound 40 exhibited cytotoxicity to both a549 ( human lung carcinoma cell line ) and jurkat cells ( human leukaemia cell line ) with ec50 values of 4.8 and 6.3  m respectively ( kanoh et  al . , 2008 ) .
f14 was isolated from seawater collected from the mangrove stand at kei ling ha lo wai , sai kung , hong kong .
chemical investigation of its fermentation broth yielded nine compounds , which were tested for antifouling activity towards macro and microfouling organisms .
antilarval activity was assessed in settlement inhibition assays against the barnacle balanus amphitrite and the bryozoan bugula neritina .
cinnamic acid ( 41 ) and bis ( 2ethylhexyl ) phthalate ( 42 ) were found to be potential natural antifouling agents inhibiting larval settlement of b. neritina ( ec50  =  77.77    14.19  m and lc50  >  1351.35  m ) and b. amphitrite ( ec50  =  23.54    4.49 
, treatment with high concentration of bis ( 2ethylhexyl ) phthalate ( 42 ) ( >  256.4  m ) was found to cause aggregation of barnacle cyprids due to possible interactions of this compound with the hydrophobic sites on cell membranes .
notably , bis(2ethylhexyl)phthalate is a common plasticizer , which was proven in this study to be produced by the fungus itself and not as a result of labware contamination .
the compounds were further tested for antibacterial activity using standard disc diffusion assay against six bacterial species , obtained from natural marine biofilms in hong kong water .
the bacterial strains included four larval settlementinducing bacteria : loktanella hongkongensis ( ust950701009 ) , micrococcus luteus ( ust950701006 ) , rhodovulum sp .
( ust010723008 ) and two marine pathogenic bacteria : pseudoalteromonas piscida ( ust010620005 ) and vibrio harveyi ( ust020129010 ) .
cinnamic acid ( 41 ) , cyclo(phepro ) ( 43 ) and cyclo(valpro ) ( 44 ) showed antibacterial activity against l. hongkongensis with mic values of 1351.35 , 819.67 and 813.0  m respectively .
cyclo(phepro ) ( 43 ) showed further antibacterial activity against m. luteus and ruegeria sp . with mics of 409.83 and 819.67  m
( strain g 100/2 ) , which was isolated in a screening programme for new marine secondary metabolites from endophytes of several types of algae , together with three known substances .
the antifungal activity of chaetocyclinone a ( 45 ) was tested against selected phytopathogenic fungi .
the compound exhibited an inhibitory activity at a dose of 89.1  m against phytophthora infestans .
the other new compounds showed neither antibacterial nor antifungal activity in a standard agar plate diffusion assay .
no cytotoxic properties against the tumour cell lines hm02 ( stomach ) , hepg2 ( liver ) and mcf7 ( breast ) could be observed up to a concentration of 28.7  m .
the results suggested the compound to be derived from the polyketide pathway ( lsgen et  al . , 2007 ) .
a new difuranxanthone , asperxanthone ( 48 ) , and a new biphenyl , asperbiphenyl ( 49 ) , were obtained from a fungal strain , identified as aspergillus sp .
( mf93 ) , which was isolated from sea water collected in quanzhou gulf , fujian province , china .
compounds 48 and 49 showed moderate inhibitory activity against tobacco mosaic virus , a typical plant virus of the tobamovirus group , with inhibitory rates of 62.9% and 35.5% , at concentrations of 0.62 and 0.48  mm , respectively ( wu et  al . , 2009 ) .
marine microorganisms are often taxonomically unique , which makes them interesting as potential sources of new drug leads
. one of the major areas of research on marine natural products is devoted to the discovery of new anticancer drugs . in 1997
, a novel depsipeptide named thiocoraline was isolated from the mycelial extract of the bacterium micromonospora marina associated with a marine soft coral in the indian ocean .
thiocoraline inhibited dna polymerase and is currently in preclinical phase by the pharmaceutical company pharmamar ( romero et  al .
768 ) , which was isolated from the red alga acanthophora spicifera , was active towards a panel of human tumour cell lines .
chemical investigation of this fungus yielded the novel macrolide apralactone a ( 50 ) , a 14membered phenyl acetic acid macrolactone , as well as six further curvularin macrolides .
the isolated compounds were tested against 36 human tumour cell lines , comprising 14 different solid tumour types .
the novel macrolide apralactone a ( 50 ) showed moderate concentrationdependent cytotoxicity with a mean ec50 value of 9.87  m .
the most active metabolite ( + ) (10e,15r)10,11dehydrocurvularin ( 51 ) , displayed concentrationdependent cytotoxicity with a mean ec50 value of 1.25  m , combined with significant in vitro tumour cell selectivity towards nine of the 36 tested tumour cell lines , which indicated 25% of selectivity ( using an individual ec50 value <  1/2 of the mean ec50 value as threshold for above average sensitivity ) .
these nine above average sensitive cell lines included bxf 1218l ( bladder cancer , ec50  =  0.43  m ) , bxf t24 ( bladder cancer , ec50  =  0.5  m ) , cnxf sf268 ( glioblastoma , ec50  =  0.36  m ) , lxfa 289l ( lung adenocarcinoma , ec50  =  0.28  m ) , maxf 401nl ( mammary cancer , ec50  =  0.4  m ) , mexf 462nl ( melanoma , ec50  =  0.38  m ) , mexf 514l ( melanoma ,
ec50  =  0.5  m ) , ovxf 899l ( ovarian cancer , ec50  =  0.58  m ) and prxf pc3 m ( prostate cancer , ec50  =  0.4  m ) ( greve et  al . , 2008 ) .
two new natural products ( z)6benzylidene3hydroxymethyl1,4dimethyl3methylsulfanylpiperazine2,5dione ( 52 ) and ( 3s,3r)3(3hydroxybutyl)7methoxyphthalide ( 53 ) , together with three known compounds were isolated from the culture broth of an unidentified marinederived fungus of the order pleosporales ( strain crif2 ) .
compound 54 , which was previously known only synthetically , was isolated for the first time as a natural product .
compounds 52 and 54 exhibited weak cytotoxic activity when tested against a panel of cancer cell lines ( prachyawarakorn et  al . , 2008 ) .
investigation of natural products produced by the marinederived fungus spicellum roseum , isolated from the sponge ectyplasia perox collected from the waters around the caribbean island of dominica , afforded two new cyclohexadepsipeptides , named spicellamide a ( 55 ) and spicellamide b ( 56 ) .
the absolute configuration of the compounds was deduced after hydrolysis using marfey 's method , chiral chromatography , as well as noesy and modelling data .
the isolated peptides were tested for their cytotoxic activity using the celltiterblue cell viability assay , against rat neuroblastoma b104 cell line .
compound 56 exhibited an ec50 value of 10.03  m while compound 55 was less active with an ec50 of 49.83  m ( kralj et  al .
( strain cnt016 ) , which was isolated from a marine mud sample collected in palau island , yielded two new secondary metabolites named spiromassaritone ( 57 ) and massariphenone ( 58 ) .
the isolated compounds were subjected to cytotoxicity assays against the human colon carcinoma cell line ( hct116 ) and also screened for antimicrobial activity . the crude extract as well as
however , none of the compounds isolated showed significant activity against hct116 , c. albicans or s. aureus ( abdelwahab et  al . , 2007 ) .
the marinederived fungus aspergillus carbonarius was isolated from a marine sediment collected at weizhou island of china .
chemical investigation of this strain when grown in liquid medium afforded two new secondary metabolites , carbonarones a ( 59 ) and b ( 60 ) .
inhibition of the human leukaemia cell lines k562 was measured by the sulforhodamine b ( srb ) assay .
the cytotoxic effects of 59 and 60 were preliminarily evaluated against k562 ( human leukaemia ) , p388 ( murine leukaemia ) , a549 ( human lung carcinoma ) , bel7402 ( human hepatoma ) and hl60 ( human promyelocytic leukaemia ) cell lines . both compounds
59 and 60 exhibited moderate antiproliferative activity against k562 cell lines with ec50 values of 244.54 and 121.39  m , respectively , while they were inactive against the other cell lines tested ( ec50  >  436.68  m ) ( zhang et  al .
, 2007 ) . the fungus aspergillus ustus was isolated from the marine sponge suberites domuncula , which had been collected from the adriatic sea . from cultures of this strain grown on both biomalt agar and barleyspelt solid media seven new drimane sesquiterpenoids ( 6167 ) , including the threoisomers 66 and 67 ,
the crude etoac extract of a. ustus displayed cytotoxic activity against the murine lymphoma cell line l5178y at a concentration of 10  g  ml .
compounds 64 , 65 and the known compound res11492 ( 68 ) exhibited ec50 values of 13.11 , 1.77 and 4.75  m , respectively , with 65 being the most active congener discovered in this study .
all other compounds were inactive at the range of concentrations analysed ( 0.110  g  ml ) .
all cytotoxic compounds featured an olefinic ester side chain comprising two ( 63 and 64 ) or three conjugated olefinic double bonds ( 68 ) with a terminal carboxylic , aldehyde or methyl substituent ( liu et  al . , 2009 ) .
the fungus petriella sp . isolated from the sponge s. domuncula that had been cultured in aquaria for 4 weeks yielded three new infectopyrone derivatives ( 6971 ) together with the cyclic tetrapeptide wf3161 ( 72 ) .
the cyclic tetrapeptide wf3161 was primarily responsible for this activity ; the ec50 value was <  0.18  m ( proksch et  al . , 2008 ) . the marinederived fungus aspergillus aculeatus cri32304a , obtained from the marine sponge xestospongia testudinaria collected from ton sai bay , phi phi islands , krabi province , thailand , afforded a new tyrosinederived metabolite , aspergillusol a ( 73 ) , in large quantities .
aspergillusol a selectively inhibited glucosidase from the yeast saccharomyces cerevisiae ( ec50  =  465    2  m ) , but it was inactive towards glucosidase from the bacterium bacillus stearothermophilus ( ec50  =  1060    20  m ) .
the compound also displayed weak cytotoxic activity towards molt3 ( acute lymphoblastic leukaemia ) , hucca1 ( human lung cholangiocarcinoma ) , and a549 cell lines with ec50 values of 19 , 50 and 74  m respectively ( ingavat et  al .
aspergiolide a ( 74 ) , a novel anthraquinone derivative with naphtho[1,2,3de]chromene2,7dione skeleton , was isolated from cultures of the marinederived fungus aspergillus glaucus .
the fungal strain was obtained from a marine sediment collected from mangrove roots in fujian province , china .
the compound selectively inhibited the proliferation of a549 , hl60 , bel7402 and p388 cancer cell lines ( du et  al . , 2007 ) .
recently , animal tests with mice indicated that aspergiolide a also inhibited tumour growth in vivo ( sun et  al . , 2009 ) .
a marinederived isolate of the common terrestrial fungus , aspergillus versicolor ( mstmf495 ) , was recovered from a beach sand sample collected at low tide from cottesloe , western australia .
the fungal strain yielded a new alkaloid , cottoquinazoline a ( 75 ) , and two new cyclopentapeptides , cotteslosins a ( 76 ) and b ( 77 ) .
cotteslosin a ( 76 ) exhibited weak cytotoxic activity against human melanoma ( mm418c5 , ec50  =  103.93  m ) , prostate ( du145 , ec50  =  141.73  m ) , and breast ( t47d , ec50  =  148.03  m ) cancer cell lines ( fremlin et  al . , 2009 ) .
fungi of the genus pestalotiopsis are characterized by their extensive distribution and wide genetic and biological diversity .
, obtained from fresh healthy leaf material of rhizophora mucronata ( rhizophoraceae ) collected in dong zhai gangmangrove garden on hainan island , china , yielded five new cytosporones j 
n ( 7882 ) , new coumarins , pestalasins a  e ( 8387 ) , a new alkaloid named pestalotiopsoid a ( 88 ) ( xu et  al .
f ( 8994 ) ( xu et  al . , 2009b ) . among the isolated compounds only pestalotiopsone f ( 94 ) exhibited moderate cytotoxicity , with an ec50 value of 26.89  m , when tested against the murine cancer cell line l5178y ( xu et  al .
three new oxaspiro[4.4]lactam containing diketopiperazine alkaloids , 6methoxyspirotryprostatin b ( 95 ) , 18oxotryprostatin a ( 96 ) and 14hydroxyterezine d ( 97 ) , as well as 14norpseurotin a ( 98 ) and the 29nordammarane triterpenoid 6,16diacetoxy25hydroxy3,7dioxy29nordammara1,17(20)dien21oic acid ( 99 ) were obtained from marinederived aspergillus sydowi pfw113 .
the fungal strain had been isolated from a driftwood sample ( pfw1 ) collected from the beach of baishamen , hainan , china .
compounds 9597 displayed weak cytotoxic activity against a549 cells , with ec50 values of 8.29 , 1.28 and 7.31  m respectively .
compound 95 also exhibited slight cytotoxicity against hl60 cells ( ec50  =  9.71  m ) .
furthermore , compounds 98 and 99 showed significant antimicrobial activities against escherichia coli , b. subtilis and micrococcus lysoleikticus with mic values of 3.74 , 14.97 and 7.49  m for compound 98 , and 10.65 , 5.33 and 10.65  m for compound 99 , respectively ( zhang et  al . , 2008b ) .
( eg5 ) , collected from waters around egypt ( suez canal , egypt ) .
chemical investigation of the fungal culture resulted in the isolation of the new chromone derivative , chromanone a ( 100 ) , which was evaluated for its cancer chemopreventive activity , especially for prevention of the initiation stage of carcinogenesis by modulation of carcinogen metabolizing enzymes .
carcinogens are activated by cytochrome p450 1a ( cyp1a ) and detoxified by glutathione stransferases ( gst ) , quinine reductase ( qr ) , and epoxide hydrolase ( meh ) .
chromanone a ( 100 ) , in 18.18  m concentration , inhibited cyp1a activity up to 60% of the stimulatedcyp1a in murine hepatoma cells ( hepa1c1c7 ) , and significantly induced gst but not total thiols at low concentrations .
the compound did not affect qr activity , but it significantly enhanced meh activity in hepa1c1c7 cells ( p  <  0.050.01 ) in a dosedependant manner . also , chromanone a ( 100 ) showed potent radical scavenging activity against hydroxyl radicals starting from a dose of 45.45  m ( p  <  0.05 ) , which may be responsible for its inhibitory effect on induced dna damage in cells ( gamaleldeen et  al . ,
diabetes mellitus is a debilitating and often lifethreatening disorder with increasing incidence throughout the world ( who , 1985 ) .
literature surveys show that more than 400 plant species were reported to have antidiabetic activity , and most of the antidiabetic natural products were , so far , isolated from plants ( mukherjee , 1981 ; rai , 1995 ) .
in contrast , marine bacteria and fungi are poorly investigated for antidiabetic activity , but may be of great promise in the search for new antidiabetic drugs for the future .
sf5060 , isolated from an intertidal sediment collected at gejae island , korea , yielded the known compound aquastatin a ( 101 ) .
the compound exhibited potent inhibitory activity against protein tyrosine phosphatase 1b ( ptp1b ) with an ec50 value of 0.19  m .
protein tyrosine phosphatases ( ptps ) constitute a large family of enzymes , which are responsible for modulation of tyrosine phosphorylationdependent cellular events .
studies demonstrated that ptp1b , an intracellular nonreceptor type ptp , negatively regulates insulin and leptinreceptor mediated signalling pathways .
thus , its inhibition may represent an outstanding , novel therapy for type 2 diabetes and obesity .
aquastatin a ( 101 ) was found to inhibit ptp1b activity in a competitive and selective manner as demonstrated by kinetic analyses and testing over a small panel of other ptps respectively .
in addition , hydrolyzing studies of the compound suggested that the dihydroxypentadecyl benzoic acid moiety present in the molecule was responsible for the inhibitory activity ( seo et  al . , 2009 ) .
table  1 contains a selection of the most active secondary metabolites isolated from marine bacteria and fungi during the period between 2007 and 2009 .
marinederived fungi and bacteria constitute a promising source of unique metabolites with considerable pharmaceutical and therapeutical potential .
common biological assays usually focus on antimicrobial and cytotoxic activities as demonstrated throughout the literature .
whereas more effective and safe drugs in the field on infectious diseases and cancer are certainly needed , many other pharmacologically active compounds may be overlooked .
thus , it is suggested to broaden biological screens for the discovery of exceptional and rarely investigated biological activities , which may be important for the therapy of chronic diseases .
examples mentioned in this review include the potent ptp1b inhibiting activity of aquastatin a ( 101 ) which may be a promising therapeutic agent for treatment of type 2 diabetes and obesity , as well as the carcinogen metabolizing enzymes modulatory activity of chromanone a ( 100 ) , which could be helpful in preventing the initiation stage of carcinogenesis .
such activities trigger the continued interest in marine microbial natural products and reflect the need for more intensive investigation of their chemical and pharmacological properties .
literature surveys showed that fungi of the genus aspergillus are among the most heavily studied fungal strains as shown in our review ( 6 investigated aspergillus species out of a total of 20 fungal species ) .
this genus is characterized by its worldwide and frequent distribution , and its species are causative agents of stored products decay , thus they are also important in view of health hazards .
nevertheless , members of this genus are renowned for their ability to produce a huge number of structurally unprecedented bioactive metabolites .
studies showed that the tendency of biosynthetic genes to form adjacent clusters proves helpful in allocating secondary metabolite genes present in fungal genomes ( keller et  al .
, 2005 , fox and howlett , 2008 ) , yet such an analysis can not predict gene expression and , thus , product formation .
recent advancements in molecular biology of fungal secondary metabolism may offer a better insight into how biogenetic gene clusters are regulated and whether their expression is affected by environmental changes and culture conditions ( delany et  al .
, 2000 ; keller et  al . , 2005 , fox and howlett , 2008 ) . a better understanding of such regulation and the influencing factors may help to induce and optimize secondary metabolite production under laboratory conditions to yield bioactive natural products with significant pharmaceutical potential .
 summarymarine bacteria and fungi are of considerable importance as new promising sources of a huge number of biologically active products . some of these marine species live in a stressful habitat , under cold , lightless and high pressure conditions . 
 surprisingly , a large number of species with high diversity survive under such conditions and produce fascinating and structurally complex natural products . up till now 
 , only a small number of microorganisms have been investigated for bioactive metabolites , yet a huge number of active substances with some of them featuring unique structural skeletons have been isolated . this review covers new biologically active natural products published recently ( 200709 ) and highlights the chemical potential of marine microorganisms , with focus on bioactive products as well as on their mechanisms of action . 