the centers for disease control and prevention estimate that more than 2 million individuals in the united states develop illness resulting from antibiotic - resistant infections on an annual basis and published antibiotic resistance threats in the united states , 2013 , which provides a snapshot of the complex problem of antibiotic resistance .
the threats were prioritized as urgent , serious , and concerning . of particular concern is increasing multidrug resistance coupled with cessation of antibiotic discovery programs by most major pharmaceutical companies .
this situation has created a major global health crisis in which there are few or no effective agents to treat common bacterial infections or infections caused by less common pathogens , including mycobacterium spp , ,   filamentous fungi , and yeasts .
, ,   furthermore , alternative second- and third - line agents that are effective are also associated with safety issues .
most current concerns about antibiotic resistance focus on infections in hospital settings requiring parenteral agents .
little is known about the activity of topical agents against multidrug - resistant organisms ( mdros ) , some of which are likely to be compromised because they contain agents to which resistance has already been reported .
these include neomycin , polymyxin b , bacitracin , and mupirocin . ,   bensal hp ( sonar products inc . ,
carlstadt , nj ) is a combination topical ointment with antimicrobial properties with activity against methicillin - resistant staphylococcus aureus ( mrsa ) and common bacterial and fungal skin pathogens .
, ,   the current study was designed to assess the in vitro activity of bensal hp against a broad range of contemporary pathogens , including mdros such as mrsa , vancomycin - resistant enterococcus , gram - negative so - called superbugs , mycobacterium fortuitum , nocardia brasiliensis , yeasts , and filamentous fungi .
bensal hp contains salicylic acid ( 30 mg / g ) , benzoic acid ( 60 mg / g ) , qrb-7 ( oak bark extract ) ( 30 mg / g ) , and vehicle polyethylene glycol 400 and polyethylene glycol 3350 .
the test agent was provided by smg pharmaceuticals , cary , north carolina . in vitro activity
was investigated against 184 bacterial and fungal isolates from the culture collections of creighton university , omaha , nebraska ; the alegent creighton hospital microbiology laboratory , omaha , nebraska ; and the university of louisville hospital microbiology laboratory , louisville , kentucky .
the bacterial isolates were from us and international sources and included well characterized non - mdro and mdro isolates of enterobacteriaceae ( n = 40 ) , pseudomonas aeruginosa ( n = 11 ) ; acinetobacter baumannii ( n = 13 ) ; s aureus ( n = 23 ) , including mrsa and methicillin - susceptible s aureus ; and enterococcus faecalis ( n = 11 ) , including vancomycin - resistant enterococcus ,
group a streptococcus ( streptococcus pyogenes [ n = 12 ] ) , propionibacterium acnes ( n = 1 ) , m fortuitum ( n = 10 ) , and n brasiliensis ( n = 10 ) .
the fungal isolates were candida albicans ( n = 10 ) , candida glabrata ( n = 10 ) , cryptococcus neoformans ( n = 1 ) , trichophyton rubrum ( n = 12 ) , t tonsurans ( n = 10 ) , and t mentagrophytes ( n = 10 ) . the gram - negative bacteria were previously characterized for resistance mechanisms by phenotypic , biochemical , and molecular methods . these included isolates of enterobacteriaceae , pseudomonas spp , and acinetobacter spp producing the extended spectrum -lactamases tem-52 , shv-4 , shv-12 , oxa-45 , ctx - m-1 , ctx - m-9 , ctx - m-12 , ctx - m-14 , ctx - m-15 , ctx - m-17 , ctx - m-18 , and ctx - m-19 ; chromosomal and plasmid - mediated ampc -lactamases , including fox - like and cmy-2 enzymes ; and carbapenemases of the imp , vim , kpc , oxa , and ndm families .
the pseudomonas aeruginosa isolates included some with upregulated mexab , mexef , and mexxy efflux pumps , and downregulation of the oprd porin .
the isolates included organisms described in the media as superbugs because of their resistance to most available antibacterial agents .
atcc reference isolates included in the study were escherichia coli atcc 25922 , escherichia coli atcc 35218 , pseudomonas aeruginosa atcc 27853 , staphylococcus aureus atcc 25923 , staphylococcus aureus atcc 29213 , enterococcus faecalis atcc 29212 , and t mentagrophytes atcc 9533 .
all isolates were tested by a cylinder diffusion procedure ,   that was a modification of the clinical and laboratory standards institute ( clsi ) disk diffusion method .
,   in this procedure a cylinder containing bensal hp was substituted for the impregnated filter paper disks of the clsi method .
bensal hp liquefied by heating to 56c for 10 minutes and 40 l was pipetted into a sterile metal cylinder placed on a lawn culture of the test organism .
the lawn culture of the test isolate was prepared according to clsi methodology and inoculated onto appropriate media ( see media ) .
the tests with gram - negative pathogens , staphylococci , streptococci , and enterococci were incubated as recommended by clsi ; that is , overnight , typically 1820 hours .
all other isolates were incubated for as long as necessary to be able to visualize sufficient growth to allow measurement of an inhibition zone ; that is , 4872 hours . after incubation ,
inhibition diameters around the cylinders were measured and recorded according to the clsi method . in the absence of clsi interpretive criteria ,
any zone of inhibition was interpreted to indicate susceptibility and the absence of an inhibition zone indicated resistance .
this interpretation was adopted to correlate with the occurrence or absence of activity at the undiluted concentration of bensal hp that is used therapeutically .
,   the test isolates were 73 representative bacterial isolates that were capable of overnight growth at 35c on mueller - hinton agar . using concentrations based on the agar dilution mics , the bactericidal activity of bensal hp against pseudomonas aeruginosa atcc 27853 and mrsa sa179
the bensal hp concentrations tested were 4  the mic and 1  the mic .
drug - free and antibiotic - supplemented mueller - hinton broths were inoculated to provide an initial inoculum of 5  10 cfu / ml of each isolate .
growth rates and killing were determined by comparing viable counts at 0 , 1 , 2 , 4 , and 24 hours .
samples for the counts were plated on roswell park memorial institute ( rpmi ) 1640 medium ( remel , lenexa , kansas ) .
this medium inhibits bensal hp activity and is therefore suitable to inactivate drug carryover in the samples .
bactericidal activity was interpreted as 3 log10 cfu / ml decrease after 24 hours of incubation .
susceptibility tests were performed on mueller - hinton agar ( bd diagnostic systems , sparks , maryland ) exception for microorganisms that did not grow well on this medium .
group a streptococci were tested on mueller - hinton agar supplemented with 5% sheep s blood ( bd diagnostic systems ) , and fungal isolates were tested on sabouraud dextrose agar ( remel ) .
initial tests of antifungal activity with rpmi 1640 medium determined that this medium antagonized the activity of bensal hp and was unsuitable for susceptibility testing .
time - kill testing was performed in mueller - hinton broth ( bd diagnostic systems ) with viable counts determined on rpmi 1640 medium .
bensal hp contains salicylic acid ( 30 mg / g ) , benzoic acid ( 60 mg / g ) , qrb-7 ( oak bark extract ) ( 30 mg / g ) , and vehicle polyethylene glycol 400 and polyethylene glycol 3350 .
in vitro activity was investigated against 184 bacterial and fungal isolates from the culture collections of creighton university , omaha , nebraska ; the alegent creighton hospital microbiology laboratory , omaha , nebraska ; and the university of louisville hospital microbiology laboratory , louisville , kentucky . the bacterial isolates were from us and international sources and included well characterized non - mdro and mdro isolates of enterobacteriaceae ( n = 40 ) , pseudomonas aeruginosa ( n = 11 ) ; acinetobacter baumannii ( n = 13 ) ; s aureus ( n = 23 ) , including mrsa and methicillin - susceptible s aureus ; and enterococcus faecalis ( n = 11 ) , including vancomycin - resistant enterococcus , group a streptococcus ( streptococcus pyogenes [ n = 12 ] ) , propionibacterium acnes ( n = 1 ) , m fortuitum ( n = 10 ) , and n brasiliensis ( n = 10 ) .
the fungal isolates were candida albicans ( n = 10 ) , candida glabrata ( n = 10 ) , cryptococcus neoformans ( n = 1 ) , trichophyton rubrum ( n = 12 ) , t tonsurans ( n = 10 ) , and t mentagrophytes ( n = 10 ) .
the gram - negative bacteria were previously characterized for resistance mechanisms by phenotypic , biochemical , and molecular methods . these included isolates of enterobacteriaceae , pseudomonas spp , and acinetobacter spp producing the extended spectrum -lactamases tem-52 , shv-4 , shv-12 , oxa-45 , ctx - m-1 , ctx - m-9 , ctx - m-12 , ctx - m-14 , ctx - m-15 , ctx - m-17 , ctx - m-18 , and ctx - m-19 ; chromosomal and plasmid - mediated ampc -lactamases , including fox - like and cmy-2 enzymes ; and carbapenemases of the imp , vim , kpc , oxa , and ndm families .
the pseudomonas aeruginosa isolates included some with upregulated mexab , mexef , and mexxy efflux pumps , and downregulation of the oprd porin .
the isolates included organisms described in the media as superbugs because of their resistance to most available antibacterial agents .
atcc reference isolates included in the study were escherichia coli atcc 25922 , escherichia coli atcc 35218 , pseudomonas aeruginosa atcc 27853 , staphylococcus aureus atcc 25923 , staphylococcus aureus atcc 29213 , enterococcus faecalis atcc 29212 , and t mentagrophytes atcc 9533 .
all isolates were tested by a cylinder diffusion procedure ,   that was a modification of the clinical and laboratory standards institute ( clsi ) disk diffusion method .
,   in this procedure a cylinder containing bensal hp was substituted for the impregnated filter paper disks of the clsi method .
bensal hp liquefied by heating to 56c for 10 minutes and 40 l was pipetted into a sterile metal cylinder placed on a lawn culture of the test organism .
the lawn culture of the test isolate was prepared according to clsi methodology and inoculated onto appropriate media ( see media ) .
the tests with gram - negative pathogens , staphylococci , streptococci , and enterococci were incubated as recommended by clsi ; that is , overnight , typically 1820 hours .
all other isolates were incubated for as long as necessary to be able to visualize sufficient growth to allow measurement of an inhibition zone ; that is , 4872 hours .
after incubation , inhibition diameters around the cylinders were measured and recorded according to the clsi method . in the absence of clsi interpretive criteria ,
any zone of inhibition was interpreted to indicate susceptibility and the absence of an inhibition zone indicated resistance .
this interpretation was adopted to correlate with the occurrence or absence of activity at the undiluted concentration of bensal hp that is used therapeutically .
,   the test isolates were 73 representative bacterial isolates that were capable of overnight growth at 35c on mueller - hinton agar .
using concentrations based on the agar dilution mics , the bactericidal activity of bensal hp against pseudomonas aeruginosa atcc 27853 and mrsa sa179 was determined by time - kill methodology .
the bensal hp concentrations tested were 4  the mic and 1  the mic .
drug - free and antibiotic - supplemented mueller - hinton broths were inoculated to provide an initial inoculum of 5  10 cfu / ml of each isolate .
growth rates and killing were determined by comparing viable counts at 0 , 1 , 2 , 4 , and 24 hours .
samples for the counts were plated on roswell park memorial institute ( rpmi ) 1640 medium ( remel , lenexa , kansas ) .
this medium inhibits bensal hp activity and is therefore suitable to inactivate drug carryover in the samples .
bactericidal activity was interpreted as 3 log10 cfu / ml decrease after 24 hours of incubation .
susceptibility tests were performed on mueller - hinton agar ( bd diagnostic systems , sparks , maryland ) exception for microorganisms that did not grow well on this medium .
group a streptococci were tested on mueller - hinton agar supplemented with 5% sheep s blood ( bd diagnostic systems ) , and fungal isolates were tested on sabouraud dextrose agar ( remel ) . initial tests of antifungal activity with rpmi 1640 medium determined that this medium antagonized the activity of bensal hp and was unsuitable for susceptibility testing .
time - kill testing was performed in mueller - hinton broth ( bd diagnostic systems ) with viable counts determined on rpmi 1640 medium .
all 184 bacterial and fungal isolates were susceptible to this combination ointment in the cylinder diffusion tests .
the susceptibility of both wild type and mdro isolates indicated that the mechanisms of resistance to other antimicrobial agents of the isolates did not compromise bensal hp activity .
inhibition zone diameters were generally larger for gram - positive bacteria and filamentous fungi than for gram - negative bacteria ( table i ) .
curiously , some mdros had larger inhibition zones than their wild type counterparts ( table i ) .
figure 1 shows 3 cylinder tests on 1 plate in which isolates of mrsa , escherichia coli , and pseudomonas aeruginosa were inhibited by this combination ointment .
the 73 isolates in the mic tests included both mdros and non - mdros for each species tested .
all gram - positive isolates were inhibited by an 80-fold dilution of bensal hp , which corresponded to salicylic acid / benzoic acid / qrb-7 concentrations of 0.375/0.75/0.375 mg / g , respectively ; that is , identical mic50 and mic90 values of 80-fold dilution of bensal hp .
the gram - negative isolates were all susceptible to a 40-fold dilution of bensal hp ( 0.75/1.5/0.75 mg / g ) , whereas mic50 and mic90 values were 80-fold and 40-fold dilutions , respectively . in time - kill tests ,
the combination ointment was rapidly bactericidal against pseudomonas aeruginosa atcc 27853 and mrsa sa179 at 4  mic .
the initial count of 2  10 cfu / ml was reduced to 800 cfu / ml by the time the inoculum was sampled and plated for the time zero reading .
that is , the bactericidal criterion of at least a 3-log reduction in viable count was achieved within approximately 5 minutes of exposure to bensal hp .
the mrsa isolate was killed with a > 4 log kill attained within an hour of exposure to 4  mic . at 1  mic ( ie , an 80-fold dilution of the clinical concentration )
the viable counts were unchanged after 24 hours . on sampling the tests after 24 hours
, there was no evidence of reduced susceptibility to bensal hp in cylinder diffusion ; that is , mutational resistance did not emerge during prolonged exposure to bensal hp .
bensal hp was active against 10 isolates each of candida albicans and candida glabrata when tested on sabouraud agar but was inactive against both species on rpmi 1640 medium .
this indicated that rpmi 1640 medium antagonized the activity of bensal hp and susceptibility tests on rpmi 1640 medium were discontinued .
on sabouraud agar , bensal hp was also active against 12 isolates of t rubrum , 10 isolates of t tonsurans , 10 isolates of t mentagrophytes , and a single isolate of cryptococcus neoformans .
figure 2 , figure 3 show the inhibition of candida albicans and t mentagrophytes , respectively , by bensal hp .
bensal hp is currently marketed and is indicated for treatment of the inflammation and irritation associated with many common forms of dermatitis , including certain eczematous conditions .
it is also used for the treatment of insect bites , burns , and fungal infections .
it has been shown to accelerate reepithelialization . this study confirmed and extended previous information about the in vitro antimicrobial spectrum of bensal hp .
the most important finding was that all 184 bacteria , yeasts , and filamentous fungi tested were susceptible to the clinically used concentration of bensal hp , indicating that it has a very broad spectrum of activity compared with other topical agents .
in addition , the finding that bensal hp was not compromised by mechanisms of antibiotic cross - resistance between it and other classes of antimicrobials is of interest . of note , multidrug - resistant acinetobacter ,
extended spectrum -lactamase  producing enterobacteriaceae , multiresistant pseudomonas aeruginosa , and mrsa were inhibited by bensal hp .
these pathogens correspond to the categories of potential pathogen threats listed in the centers for disease control and prevention report .
the results from our study suggest that bensal hp may provide an effective topical treatment in situations where mdros are problematic .
the activity of bensal hp against m fortuitum also raises the possibility of activity against other mycobacterium species , some of which are highly drug - resistant .
in addition , the absence of mutational resistance emerging during prolonged exposure in the time - kill tests suggested that pathogens may not easily develop resistance to bensal hp .
additional investigation is needed on the mechanism of action of bensal hp and whether the antimicrobial activity results from the individual components or whether the combination is required to demonstrate these effects .
further studies are warranted to investigate the potential prophylactic , decolonization , and therapeutic uses of bensal hp .
it would also be useful to compare its activity to other topical agents such as the combinations of bacitracin / neomycin / polymyxin , and bacitracin / polymyxin , and the monocomponent agents , mupirocin , and silver sulfadiazine .
a limitation of this study is that there is neither a standardized susceptibility test method nor interpretative criteria for topical ointments such as bensal hp , with the exception of mupirocin , which is water - soluble . in the absence of such methodology , the cylinder test method using the clinically applied concentration provided useful information .
bensal hp is a very - broad - spectrum topical antimicrobial agent with in vitro activity against important pathogens such as mrsa , pseudomonas aeruginosa , gram - negative superbugs , yeasts , and filamentous fungi .
based on the in vitro findings in this study , additional studies are warranted to better understand the full clinical utility of this agent .
k. thomson has received a research grant and honoraria from smg and g. thomson as received honoraria from smg .
the authors have indicated that they have no other conflicts of interest regarding the content of this article .
 backgroundthe use of topical antimicrobial agents for management of minor skin infections is a clinical strategy that is commonly practiced in the community . coupled with the use of topical antimicrobial agents 
 is the emergence of antibiotic - resistant strains of pathogens leading to the need for alternative treatments.objectivea novel topical combination ointment consisting of salicylic acid , oak bark extract , benzoic acid , and polyethylene glycol ( bensal hp , sonar products inc . , 
 carlstadt , nj ) with antimicrobial properties was assessed to determine its spectrum of activity.methodsone hundred eighty - four bacterial and fungal isolates from culture collections that included multidrug - resistant bacteria such as methicillin - resistant staphylococcus aureus , pseudomonas aeruginosa , acinetobacter spp , and gram - negative so - called superbugs , as well as yeasts and filamentous fungi , were investigated by cylinder diffusion and agar dilution assays.resultsall 184 bacterial and fungal isolates were susceptible to the combination ointment at the clinically applied concentration and there was no evidence of cross - resistance between bensal hp and other classes of antimicrobials . in time - kill tests , 
 bensal hp was rapidly bactericidal against p aeruginosa atcc 27853 and methicillin - resistant s aureus sa179 at 4  the mic , a concentration that is applied clinically.conclusionsthe results of this study suggest that this combination ointment has a broad in vitro spectrum of antimicrobial activity against both more common bacterial and fungal pathogens and may be particularly useful for treatment of infections by multidrug - resistant organisms . 
 additional studies are warranted to investigate the full clinical utility as a therapeutic agent and also for possible infection control interventions . 