to date only two species , mangrovibacter plantisponsor dsm 19579   and mangrovibacter yixingensis kctc 42181   have been described under the genus mangrovibacter ( http://www.bacterio.net/mangrovibacter.html ) . the draft genome of mangrovibacter sp . strain mfb070 , isolated from an aquaculture farm in india has been described recently   which is the only available genome sequence within genus mangrovibacter .
members of the genus mangrovibacter have been shown to possess plant growth promoting features such as nitrogen fixation   and may provide niche - specific advantage to the plant . in context to invasive weeds , this could provide a better ecological fitness in an invaded territory compared to native vegetation .
thus , understanding the microbiota and role they may play during plant invasion could lead to more directed and sustainable management of weeds .
chilika lagoon ( 1928-1954n ; 8506-8535e ) is a brackish water lagoon located in the odisha state of india .
p. karka is a large perennial grass of the family poaceae and occupy most of the northern shoreline of lagoon . in order to understand the microbial basis of the invasive success of this weed
, a study was undertaken to investigate the culturable diversity of rhizosphere microbiota associated with p. karka . during this study , a gamma - proteobacterium , facultative anaerobic , endophytic nitrogen fixing mangrovibacter sp .
strain mp23 grew at temperatures between 20  c and 40  c with an optimum at 37  c in presence of 1% nacl .
the 16s rrna gene phylogenetic analysis showed that strain mp23 was most closely ( 99.71% similarity ) related to m. yixingensis kctc 42181 and m. plantisponsor dsm 19579 indicating that it belong to genus mangrovibacter . here , we described the draft whole genome shotgun sequence of strain mangrovibacter sp .
strain mp23 ( dsm 100250  =  kctc 42580 ) , which will provide genetic insights into the nitrogen fixation , stress tolerance , plant niche adaptation , and comparative evolution of this species .
the genome of mangrovibacter sp . strain mp23 was sequenced by a shotgun sequencing method using the illumina miseq sequencing system with a paired - end module .
the ngs qc toolkit v 2.3   was used to filter the data for high - quality ( hq ) vector- and adapter - free reads for genome assembly .
a total of 6,895,374 paired end reads were generated out of which 6,702,332 high quality vector - filtered reads were considered , representing approximately 310 fold coverage of the genome .
these reads were assembled using masurca v. 3.1.3   and resulted into 50 contigs with a total size of 4,947,475  bp and an n50contig length of 428,946  bp .
annotations of protein - coding genes , as well as other functional genome units were carried out through ncbi prokaryotic genome annotation pipeline ( pgap ) .
the genome contained a total of 4592 genes and 4392 protein - coding genes with predicted function .
the complete genome of strain mp23 was 4,947,475  bp in length with an estimated g  +  c content of 49.9% and consists of 77 trna genes and 28 rrna ( 8  =  5s , 5  =  16s , 15  =  23s ) genes ( table 1 ) .
this whole genome shotgun project has been deposited at ddbj / embl / genbank under the accession number lyrp00000000 .
the authors declare that there is no conflict of interests with respect to the work published in this paper .
 till date , only one draft genome has been reported within the genus mangrovibacter . here , we report the second draft genome shotgun sequence of a mangrovibacter sp . 
 strain mp23 that was isolated from the roots of phargmites karka ( p. karka ) , an invasive weed growing in the chilika lagoon , odisha , india . strain mp23 is a facultative anaerobic , nitrogen - fixing endophytic bacteria that grows optimally at 37  c , 7.0 ph , and 1% nacl concentration . 
 the draft genome sequence of strain mp23 contains 4,947,475  bp with an estimated g  +  c content of 49.9% and total 4392 protein coding genes . 
 the genome sequence has provided information on putative genes that code for proteins involved in oxidative stress , uptake of nutrients , and nitrogen fixation that might offer niche specific ecological fitness and explain the invasive success of p. karka in chilika lagoon . the draft genome sequence and annotation 
 have been deposited at ddbj / embl / genbank under the accession number lyrp00000000 . 