infections are common in patients with cirrhosis and have been found in up to 66% of patients with variceal hemorrhage .
prophylactic treatment with third generation cephalosporins is recommended in patients with advanced cirrhosis and gastrointestinal bleeding to cover for the predominant causative gut flora organisms , .
treatment is recommended even if no causative organism is identified as up to 50% of patients with cirrhosis and clinical sepsis have negative cultures . identifying appropriate antimicrobials
can be challenging without a causative organism , and the empiric use of broad spectrum antimicrobials contributes to multidrug resistant organisms .
we report the first known case of paracoccus yeeii bacteremia in a patient with decompensated cirrhosis who presented with variceal hemorrhage . this rare gram negative organism may not be susceptible to third generation cephalosporins .
this finding may have implications for the management of infected but culture negative patients with cirrhosis .
a 42  year old gentleman with decompensated cirrhosis from genotype 1a ( g1a ) hepatitis c ( hcv ) had been treated with 24 weeks of ledipasvir and sofosbuvir which ended 4.5 months earlier .
the patient presented with new onset jaundice ( total bilirubin 23  mg / dl , direct bilirubin 18  mg / dl , alkaline phosphatase 99  u / l , alanine aminotransferase 31  u / l , aspartate aminotransferase 86  u / l ) that peaked to total bilirubin 44  mg / dl and direct bilirubin 31  mg / dl . model for end - stage liver disease ( meld )
score on admission was 27 . given the report of subjective fevers in the setting of recently treated k. pneumoniae bacteremia of unknown source , admission blood and urine cultures were obtained but were without growth .
molecular studies revealed hcv relapse ( hcv viral load  =  4.44 log iu / ml ) without ns5b resistance ( insufficient sample for ns5a ) and undetectable hepatitis b ( hbv ) and adenovirus .
there was no serologic evidence of acute infection with hepatitis e , cytomegalovirus ( cmv ) , or epstein barr virus ( ebv ) .
liver biopsy demonstrated cirrhosis with moderate chronic inflammation , focal severe neutrophilic infiltrates with cholestasis , and ductular proliferation .
magnetic resonance imaging ( mri ) and magnetic resonance cholangiopancreatography ( mrcp ) showed no cholangitis or abscess . although drug induced liver injury from ciprofloxacin was clinically suspected , two sets of blood cultures were obtained daily due to concern for infection and lack of clinical improvement . the patient elected discharge but was readmitted within 24  hours with hematemesis from variceal hemorrhage and portal hypertensive gastropathy .
empiric treatment for undifferentiated septic shock including spontaneous bacterial peritonitis prophylaxis were ceftriaxone 2  g iv every 24  hours , metronidazole 500  mg iv every 8  hours , and vancomycin 2  g iv every 12  hours .
diagnostic paracentesis was again attempted but the patient had minimal ascites . given the lack of clinical improvement and the finding of bowel edema and severe colitis on computer tomography , infectious disease was consulted .
it was recommended to adjust antimicrobials to piperacillin - tazobactam 3.375  mg every 6  hours , metronidazole 500  mg iv every 8  hours , and vancomycin 125  mg po every 6  hours to cover for gut flora including clostridium difficile until it could be ruled out .
blood cultures collected the day before discharge of the previous admission flagged as  positive  after 5  days of incubation in the aerobic bottle ( bactec plus aerobic / f ) , but no organisms were seen upon gram stain . however , after two days of incubation , small mucoid colonies of oxidase positive gram negative coccobacilli were seen growing on chocolate agar only . the team was notified and antimicrobials were adjusted to only piperacillin - tazobactam 4.5  g every 6  hours .
after an additional two days of incubation , an identification of paracoccus yeeii was made by matrix assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ; vitek ms ) with a match of 99.9% to the vitek ms ivd unclaimed database .
identification was confirmed by sequencing of the first 500  bp of the 16s gene . upon speciation ,
the patient was switched to ceftazidime 1  g every 12  hours to cover for p. yeeii as well as concern for a hospital acquired pneumonia .
the patient completed a 2 week treatment course of gram negative antimicrobial coverage with two beta lactam agents and had clinical improvement with no other culture growth of p. yeeii .
unfortunately , the patient developed end - stage liver disease and eventually succumbed to death due to septic shock from citrobacter freundii pneumonia .
to our knowledge , this is the first reported case of p. yeeii in a patient with cirrhosis .
patients with decompensated cirrhosis are at high risk of infection from immune dysregulation , a heightened inflammatory response , altered gut flora , and bowel translocation .
however , no causative organism is found in up to 50% of patients with decompensated cirrhosis presenting with clinical sepsis .
this case of bacteremia with a rare gram negative organism is instructive regarding optimal diagnosis and treatment of infection in patients with decompensated cirrhosis who have an unclear source of infection as they have an extremely high risk of morbidity and mortality .
p. yeeii is an aerobic gram negative coccobacillus that has been isolated in soil and marine sediment , and has been rarely reported as infections in humans .
p. yeeii has been identified in the past through commonly available biochemical tests , and this case demonstrates the utility of maldi - tof ms for the identification of this infrequently encountered organism , .
the few reported cases of p. yeeii to date have entailed peritonitis among peritoneal dialysis patients , , myocarditis in a heart transplant patient , corneal graft infection , and heart failure with bullous skin lesions .
all cases identified p. yeeii through culture of affected tissues . only 2 cases , including ours , have been associated with bacteremia and ours is the first case of a patient with cirrhosis . the source of bacteremia in our case remains unclear , but it is plausible that the patient s lymphedema and bowel edema could be sources for this environmentally found organism . over the previous year , the patient had several episodes of bacteremia thought to be from bowel translocation including group g -hemolytic streptococci , streptococcus mitis / oralis , citrobacter braakii , and enterococcus faecium ( the patient was not on chronic antimicrobials ) .
maldi - tof may help with rapid diagnosis and guide appropriate treatment when initial cultures in patients with decompensated cirrhosis and clinical sepsis are negative .
it is recommended that patients with advanced cirrhosis who develop gastrointestinal bleeding be treated empirically with third generation cephalosporins , .
however , this case illustrates that uncommon gram negative organisms may not necessarily be covered with third generation cephalosporins .
although we did not have antimicrobial susceptibilities performed in our case , the existing literature on p. yeeii suggests that the organism is susceptible to fluoroquinolones and beta - lactams though higher minimum inhibitory concentrations ( mics ) were reported with third generation cephalosporins .
this case reinforces the challenges in isolating rare infections in patients with cirrhosis . in the absence of obvious infectious sources in a patient with decompensated cirrhosis
collaboration with infectious disease specialists and microbiologists is warranted in these cases to isolate infectious organisms and to identify appropriate antimicrobials promptly .
this research did not receive any specific grant from funding agencies in the public , commercial , or not - for - profit sectors .
 highlightsno organism is found in up to 50% of infected patients with decompensated cirrhosis.in the absence of obvious infectious sources , consider rare gram negative organisms.uncommon gram negative organisms may not be covered by empiric antibiotics.maldi-tof may help with diagnosis when cultures are negative in these patients.human infections with paracoccus yeeii , a gram negative coccobacillus , is rare . 