Cancer cachexia (CC) accounts for 20%–40% of cancer-related deaths. Mitochondrial aberrations have been shown to precede muscle atrophy in different atrophy models, including cancer. Therefore, this study investigated potential protection from the cachectic phenotype through overexpression of peroxisome proliferator-activated receptorγcoactivator-1 α (PGC-1α). First, to establish potential of mitochondria-based approaches we showed that the mitochondrial antioxidant MitoTEMPO (MitoT) attenuates myotube atrophy induced by Lewis lung carcinoma (LLC) cell conditioned media. Next, cachexia was induced in muscle-specific PGC-1α overexpressing (MCK-PCG1α) or wildtype (WT) littermate mice by LLC implantation. MCK-PCG1α did not protect LLC-induced muscle mass loss. In plantaris,AtroginmRNA content was 6.2-fold and ~11-fold greater in WT-LLC vs WT-phosphate-buffered saline (PBS) for males and females, respectively (p< 0.05). MitoTimer red:green ratio for male PGC was ~65% higher than WT groups (p< 0.05), with ~3-fold more red puncta in LLC than PBS (p< 0.05). Red:green ratio was 56% lower in females WT-LLC vs PGC-LLC (p< 0.05). In females, no change in red puncta was noted across conditions.Lc3mRNA content was ~73% and 2-fold higher in male and female LLC mice, respectively, vs PBS (p< 0.05). While MitoT could mitigate cancer-induced atrophy in vitro, PGC-1α overexpression was insufficient to protect muscle mass and mitochondrial health in vivo despite mitigation of cachexia-associated signaling pathways.