BackgroundThe existing ex vivo models of endoscopic submucosal dissection (ESD) cannot simulate intraoperative hemorrhage well. We aimed to establish an ESD training method by applying an ex vivo training model with continuous perfusion (ETM-CP).MethodsFour training sessions were conducted for 25 novices under the guidance of 2 experts. Eventually, 10 novices completed ESD operations on a total of 89 patients after the training. The resection effectiveness, resection speed, complication rate, and novice performance before and after the training were compared. The data regarding the effects of the training and the model were gathered through a questionnaire survey.ResultsIn terms of the simulation effect of the model, ETM-CP was evaluated as similar to the live pig in all aspects (P> 0.05). The questionnaire analysis revealed that the ESD theoretical knowledge, skill operation, and self-confidence of novices were improved after the training (P< 0.05). The resection time per unit area had a correlation with the number of training periods (rs = – 0.232). For novice performance, the resection time per unit area was shortened (P< 0.05). There was no difference in patient performance between the novice group and the expert group after the training in terms of en bloc resection, R0 resection, complication rate, endoscopic resection bleeding (ERB) score, muscularis propria injury (MPI) score, and resection time per unit area (P> 0.05).ConclusionThe ETM-CP is effective for ESD training.Supplementary InformationThe online version contains supplementary material available at 10.1007/s00464-023-09940-9.