GenusParishas numerous bioactive constituents such as steroid saponins, flavonoids, and polysaccharose which are responsible for antitumor, hemostatic, and anthelmintic, etc. In this study, ultrahigh performance liquid chromatography coupled to time-of-flight mass spectrometer (UHPLC-QTOF-MS) and Fourier transform infrared (FT-IR) spectroscopy in combination with multivariable analysis were employed to discriminate the different species ofParisincludingP. polyphyllavar.yunnanensis(PPY),P. polyphyllavar.alba,P. mairei(PM),P. vietnamensis, andP. polyphyllavar.stenophylla. Partial least square discriminate analysis based on UHPLC, FT-IR, and midlevel data fusion was used to distinguish 43 batches of Paris. Chemical constituents of different speciesPariswere determined by UHPLC-QTOF-MS. The result indicated that midlevel data fusion had a good performance in the classification compared to a single analytical technology. A total of 47 compounds were identified in different speciesParis. The similar results indicated that PM could be treated as a proposal substitute of PPY.