The application ofTrichodermais a form of biological control that has been effective in combatingXanthomonas oryzaepv.oryzae, the causative agent of the devastating disease known as bacterial blight of rice. In this present study, four strains ofTrichoderma,viz.,T. paraviridescens(BDISOF67), T. erinaceum(BDISOF91),T. asperellum(BDISOF08), andT. asperellum(BDISOF09), were collected from the rice rhizosphere and used to test their potentiality in reducing bacterial blight. The expression patterns of several core defense-related enzymes and genes related to SA and JA pathways were studied to explore the mechanism of induced resistance by thoseTrichodermastrains. The results primarily indicated that allTrichodermawere significantly efficient in reducing the lesion length of the leaf over rice check variety (IR24) through enhancing the expression of core defense-related enzymes, such as PAL, PPO, CAT, and POD activities by 4.27, 1.77, 3.53, and 1.57-fold, respectively, over control. Moreover, the results of qRT-PCR exhibited an upregulation of genesOsPR1,OsPR10,OsWRKY45,OsWRKY62,OsWRKY71,OsHI-LOX, andOsACS2after 24 h of inoculation with all testedTrichodermastrains. However, in the case of RT-PCR, no major changes inOsPR1andOsPR10expression were observed in plants treated with differentTrichodermastrains during different courses of time. Collectively,Trichodermainduced resistance in rice againstX. oryzaepv.oryzaeby triggering these core defense-related enzymes and genes associated with SA and JA pathways.