Background: The eustachian tube (ET) is a connection between the nasopharynx and the middle ear behind the inferior nasal concha. It plays an important role in regulating air pressure across the tympanic membrane for proper transmission of sound. The pharyngeal opening of the tube is an important landmark for endoscopic evaluation in patients suffering from chronic otitis media and is also an important anatomical landmark for the transnasal approach to the infratemporal fossa. Hence, the study was done to locate the position of the pharyngeal opening of the ET in relation to various important anatomical landmarks.Methodology: Hundred (50 right and 50 left sides) adult (60-80 years) formalin-fixed sagittal sections of head and neck specimens were taken for the study, which was obtained during the undergraduate teaching program. The shape, size, and position of the pharyngeal opening of the ET were noted. The distance between the pharyngeal opening of the ET and various anatomical landmarks was measured with the help of the digital Vernier caliper. The mean and standard deviation of all the parameters were calculated and tabulated.Results: In the present study, a slit-like shape was the most common shape of the pharyngeal opening, present in 62 out of 100 specimens. The difference between the anteroposterior length and vertical height of the two sides showed a statistically significant difference.Conclusion: The present study will help to locate the position of the pharyngeal opening of the ET during otorhinolaryngological evaluation for performing various surgeries in the middle ear.