The article describes the results of the online survey on open science (OS) carried out on researchers affiliated with universities and Spanish research centres and focused on open access to scientific publications, the publication process, the management of research data and the review of open articles. The main objective was to identify the perception and habits of researchers with regard to practices closely linked to open science and the scientific value added is that offers an in-depth picture of researchers as one of the main actors to whom this transformation and implementation of open science will fall. It focuses on the different aspects of OS: open access, open data, publication process and open review in order to identify habits and perceptions. This is to make possible an implementation of the OS movement. The survey was carried out among researchers who had published in the years 2020–2021, according to data obtained from WoS. It was emailed to a total of 8,188 researchers and obtained a total of 666 responses, of which 554 were complete, the rest being forms with some questions unanswered. The main results showed that open access still requires the diffusion of practices and services provided by the institution, as well as training (library or equivalent service) and institutional support from the competent authorities (vice rectors or equivalent) in specific aspects such as data management. In the case of data, around 50% of respondents stated they had stored data in a repository, and of all the options, the most frequently given was that of an institutional repository, followed by a discipline repository. Among the main reasons for doing this, we found transparency, visibility of data and the ability to validate results. For those who stated they had never stored data, the most frequent reasons for not having done so were privacy and confidentiality, the lack of a mandated data policy or a lack of knowledge of how to do it. In terms of open peer review, participants mentioned a certain reticence to the opening of evaluations due to potential conflicts of interest that may arise or because lower-quality content might be accepted in order to avoid conflicts. In addition, the hierarchical structure of senior researcher versus junior researcher might affect reviews. The main conclusions indicate a need for persuasion of OA to take place; APCs are an economic barrier rather than the main criterion for journal selection; OPR practices may seem innovative and emerging; scientific and evaluation policies seem to have a clear effect on the behaviour of researchers; researchers state that they share research data more for reasons of persuasion than out of obligation. Researchers do question the pathways or difficulties that may arise on a day-to-day basis and seem aware that we are undergoing change, where academic evaluation or policies related to open science, its implementation and habits among researchers may change. In this sense, more and better support is needed on the part of institutions and faculty support services.