Introduction: Considering people’s tendency to self-treatment, efforts to address the broad aspects of health literacy are extremely important. The study aimed to find out the level of health literacy related to the use of retinol creams among female undergraduate students of the Faculty of Artificial Intelligence at Al-Balqa Applied University.Methods: This study adopted an analytical descriptive research methodology by designing and applying a questionnaire tool. The questionnaire consisted, after arbitration and testing its validity and stability, of 15 items. Each item represents one of the indicators for measuring the level of health literacy about the use of retinol creams. The sample of the study was a random sample of female students in the Faculty of Artificial Intelligence at Al-Balqa Applied University.Results: A total of 221 undergraduate female students were enrolled in the study. The most important results were that the arithmetic mean for estimating the level of health culture among female students in the study on the use of retinol creams was 3.117 out of 5, with a relative weight percentage of 62.3% and an average total score on the general level of indicators of total health culture.Conclusion: This study provided insights into the health literacy related to the use of retinol creams among female students. While the students demonstrated a high level of health education in some aspects, there were areas where their knowledge and practices could be improved. These findings can contribute to the development of educational programs and interventions aimed at promoting the safe and informed use of retinol creams among university students.