Introduction: In our study, we aimed to investigate the effect of the previous delivery route on uterine artery pulsatility index (PI) and obstetric outcome. We aim to assess the effects of previous cesarean delivery (CD) and placental location on first- and second-trimester uterine artery Doppler indices as well as first-trimester pregnancy-associated plasma protein-A (PAPP-A) multiples of the median (MoM) levels in the subsequent pregnancy.Materials and methods: We designed a retrospective cohort study to collect the participants’ clinical and uterine artery Doppler. Data regarding pregnant women’s first- and second-trimester exams, who were referred to our maternal-fetal medicine unit, were collected from hospital records between June 2015 and December 2019.Results: Uterine artery PI MoM values were not different between the cases with the anterior and non-anterior placental locations. No significant difference was found in the first- and second-trimester uterine artery PI MoM values by delivery route (p = 0.57). However, the intrauterine growth restriction rate was higher in the CD group (p < 0.001).Conclusion: In this study, we compared the uterine blood flow indices between the previous cesarean and vaginal delivery groups. We observed no significant difference between the patients with different delivery routes.