Haemoproteus columbaeis a common haemosporidian parasite of wild pigeons (Columba livia) reported worldwide. In Thailand, the wild pigeon population is increasing due to paddy field monoculture. However, there are limited reports on the presence ofH. columbaein these pigeon populations. The aim of the study was to characterizeH. columbaein wild pigeons. A total of 87 wild pigeons were examined using microscopic and molecular methods.Haemoproteus columbaewas detected in approximately 27.6% of pigeons and their morphological characteristics were described. The partial cytochromeb(cyt b) gene sequence ofH. columbaewas then characterized into three common lineages (HAECOL1, COLIV03, and COQUI05). By highlighting the morphologic and genetic characteristics ofH. columbaecommonly found in this population of pigeons, this study provides essential regional knowledge about haemosporidian parasites that could benefit future taxonomic and phylogeographic studies.