This study examined potential sex-related differences and correlations among the pennation angle (PA), muscle thickness (MT), and mechanomyographic amplitude (MMGRMS)–torque relationships of the vastus lateralis (VL) in 11 healthy males and 12 healthy females. The PA and MT of the VL were quantified with ultrasound. Participants performed an isometric muscle action of the knee extensors that linearly increased to 70% of maximal strength followed by a 12 s plateau. MMG was recorded from the VL. Linear regression models were fit to the log-transformed MMGRMS–torque relationships to calculatebterms (slopes) for the linearly increasing segment. MMGRMSwas averaged during the plateau. Males exhibited greater PA (p< 0.001), MT (p= 0.027),bterms (p= 0.005), and MMGRMS(p= 0.016). Thebterms were strongly (p< 0.001,r= 0.772) and moderately correlated (p= 0.004,r= 0.571) with PA and MT, respectively, while MMGRMSwas moderately correlated with PA (p= 0.018,r= 0.500) and MT (p= 0.014,r= 0.515). The greater mechanical behavior of individuals possessing a larger PA and MT of the VL may reflect increased cross-bridge activity within the muscle fibers. Additionally, PA may help explain sex-related differences in MMGRMSbetween sexes.