Transposition of transposable elements affect expression levels, splicing and epigenetic status, and function of genes located in, or near, the inserted/excised locus. For example, in grape, presence of theGret1retrotransposon in the promoter region of theVvMYBA1aallele at theVvMYBA1locus suppress the expression of theVvMYBA1transcription factor gene for the anthocyanin biosynthesis and this transposon insertion is responsible for the green berry skin color ofVitis labrascana, ‘Shine Muscat’, a major grape cultivar in Japan. To prove that transposons in grape genome can be removed by genome editing, we focused onGret1in theVvMYBA1aallele as a target of CRISPR/Cas9 mediated transposon removal. PCR amplification and sequencing detectedGret1eliminated cells in 19 of 45 transgenic plants. Although we have not yet confirmed any effects on grape berry skin color, we were successful in demonstrating that cleaving the long terminal repeat (LTR) present at both ends ofGret1can efficiently eliminate the transposon.