Blumeria graminis forma specialis tritici(B.g. tritici) is the airborne fungal pathogen that causes powdery mildew disease on hexaploid bread wheat. Calmodulin-binding transcription activators (CAMTAs) regulate plant responses to environments, but their potential functions in the regulation of wheat–B.g. triticiinteraction remain unknown. In this study, the wheat CAMTA transcription factors TaCAMTA2 and TaCAMTA3 were identified as suppressors of wheat post-penetration resistance against powdery mildew. Transient overexpression ofTaCAMTA2andTaCAMTA3enhanced the post-penetration susceptibility of wheat toB.g. tritici, while knockdown ofTaCAMTA2andTaCAMTA3expression using transient- or virus-induced gene silencing compromised wheat post-penetration susceptibility toB.g. tritici.In addition,TaSARD1andTaEDS1were characterized as positive regulators of wheat post-penetration resistance against powdery mildew. OverexpressingTaSARD1andTaEDS1confers wheat post-penetration resistance againstB.g. tritici, while silencingTaSARD1andTaEDS1enhances wheat post-penetration susceptibility toB.g. tritici.Importantly, we showed that expressions ofTaSARD1andTaEDS1were potentiated by silencing ofTaCAMTA2andTaCAMTA3.Collectively, these results implicated that theSusceptibilitygenesTaCAMTA2andTaCAMTA3contribute to the wheat–B.g. triticicompatibility might via negative regulation ofTaSARD1andTaEDS1expression.