Botrytis cinerea, a plant pathogenic fungus with a wide host range, has reduced sensitivity to fungicides as well as phytoalexins, threatening cultivation of economically important fruits and vegetable crops worldwide.B. cinereatolerates a wide array of phytoalexins, through efflux and/or enzymatic detoxification. Previously, we provided evidence that a distinctive set of genes were induced inB. cinereawhen treated with different phytoalexins such as rishitin (produced by tomato and potato), capsidiol (tobacco and bell pepper) and resveratrol (grape and blueberry). In this study, we focused on the functional analyses ofB. cinereagenes implicated in rishitin tolerance. LC/MS profiling revealed thatB. cinereacan metabolize/detoxify rishitin into at least 4 oxidized forms. Heterologous expression of Bcin08g04910 and Bcin16g01490, twoB. cinereaoxidoreductases upregulated by rishitin, in a plant symbiotic fungusEpichloë festucaerevealed that these rishitin-induced enzymes are involved in the oxidation of rishitin. Expression ofBcatrB, encoding an exporter of structurally unrelated phytoalexins and fungicides, was significantly upregulated by rishitin but not by capsidiol and was thus expected to be involved in the rishitin tolerance. Conidia ofBcatrBKO (ΔbcatrB) showed enhanced sensitivity to rishitin, but not to capsidiol, despite their structural similarity.ΔbcatrBshowed reduced virulence on tomato, but maintained full virulence on bell pepper, indicating thatB. cinereaactivatesBcatrBby recognizing appropriate phytoalexins to utilize it in tolerance. Surveying 26 plant species across 13 families revealed that theBcatrBpromoter is mainly activated during the infection ofB. cinereain plants belonging to the Solanaceae, Fabaceae and Brassicaceae. TheBcatrBpromoter was also activated byin vitrotreatments of phytoalexins produced by members of these plant families, namely rishitin (Solanaceae), medicarpin and glyceollin (Fabaceae), as well as camalexin and brassinin (Brassicaceae). Consistently,ΔbcatrBshowed reduced virulence on red clover, which produces medicarpin. These results suggest thatB. cinereadistinguishes phytoalexins and induces differential expression of appropriate genes during the infection. Likewise, BcatrB plays a critical role in the strategy employed byB. cinereato bypass the plant innate immune responses in a wide variety of important crops belonging to the Solanaceae, Brassicaceae and Fabaceae.