Simple SummaryMonitoring non-native species is important to assess their invasion risk. Here, we carried out a literature review on roadkill studies to investigate geographical patterns of biological invasions. We believe that roadkill data from published literature can be a valuable resource for people dealing with biological invasions, especially when field surveys cannot be performed. We retrieved 2314 studies published until January 2022, but only 40 of them (in addition to our own unpublished field data) included a full list of road-killed species (with a number of affected individuals for each species), so we were able to include them in our analyses. We classified all road-killed species from retrieved studies as native or introduced (domestic, introduced in historical times or recently released). We found that a higher number of introduced species would be recorded among roadkill in Mediterranean and Temperate regions with respect to Tropical and Desert areas. This result is in line with the current knowledge on non-native species distribution at the global scale, thus confirming that roadkill datasets can be used beyond the study of road impacts, such as for an assessment of different levels of biological invasions among different countries.AbstractMonitoring the presence and distribution of alien species is pivotal to assessing the risk of biological invasion. In our study, we carried out a worldwide review of roadkill data to investigate geographical patterns of biological invasions. We hypothesise that roadkill data from published literature can turn out to be a valuable resource for researchers and wildlife managers, especially when more focused surveys cannot be performed. We retrieved a total of 2314 works published until January 2022. Among those, only 41 (including our original data) fitted our requirements (i.e., including a total list of roadkilled terrestrial vertebrates, with a number of affected individuals for each species) and were included in our analysis. All roadkilled species from retrieved studies were classified as native or introduced (domestic, paleo-introduced, or recently released). We found that a higher number of introduced species would be recorded among roadkill in Mediterranean and Temperate areas with respect to Tropical and Desert biomes. This is definitely in line with the current knowledge on alien species distribution at the global scale, thus confirming that roadkill datasets can be used beyond the study of road impacts, such as for an assessment of different levels of biological invasions among different countries.