Currently, natural materials represent a sustainable option for the manufacture of biopolymers with numerous industrial applications and characteristics comparable with synthetic materials. Nopal mucilage (NM) is an excellent natural resource for the synthesis of bioplastics (BPs). In the present research, the fabrication of biopolymers by using NM is addressed. Changes in the plasticizer (sorbitol and cellulose) concentration, in addition to the implementation of two sources of starch (corn starch (CS) and potato starch (PS)) to obtain the surgical thread, were analyzed. The NM extracted was close to 14% with ethanol. During the characterization of the extract, properties such as moisture, humidity, viscosity, and functional groups, among others, were determined. In the CS and PS analysis, different structures of the polymeric chains were observed. BP degradation with different solvents was performed. Additionally, the addition of sorbitol and cellulose for the BP mixtures presenting the highest resistance to solvent degradation and less solubility to water was conducted. The obtained thread had a uniform diameter, good elasticity, and low capillarity compared to other prototypes reported in the literature.