The cost of academic publishing has increased substantially despite the ease with which information can be shared on the web. Open Access publishing is a key mechanism for amplifying research access, inclusivity, and impact. Despite this, shifting to a free-to-read publishing environment requires navigating complex barriers that vary by career status and publishing expectations. In this article, we investigate the motivations and preferences of researchers situated within our large research institution as a case study for publishing attitudes at similar institutions. We surveyed the publishing priorities and preferences of researchers at various career stages in STEM fields as they relate to openness, data practices, and assessment of research impact. Our results indicate that publishing preferences, data management experience and research impact assessment vary by career status and departmental approaches to promotion. We find that open access publishing is widely appreciated regardless of career status, but financial limitations and publishing expectations were common barriers to publishing in Open Access journals. Our findings shed light on publishing attitudes and preferences among researchers at a major R1 research institution, and offer insight into advocacy strategies that incentivize open access publishing.