IntroductionSaussurea costus, an important critically endangered medicinal herb native to the Himalayan region, is commonly used in various ailments,viz. asthma, ulcer, inflammation, and stomach problems. In the international market, the dry roots and essential oil ofS. costushas become an important drug. The lack of appropriate fertilizer dose recommendations is one of the limiting factors for itsex-situconservation and large-scale cultivation, as plant nutrition is vital in determining crop growth and productivity. The study aimed to understand the comparative impact of different levels of fertilizer nutrients on growth, dry root and essential oil yield, and essential oil profile ofS. costus.MethodsA field experiment was conducted in Himachal Pradesh, India's cold desert region (Lahaul valley), during 2020-21. The experiment comprised three levels of nitrogen (60, 90, and 120 kg ha-1), three levels of phosphorus (20, 40, 60 kg ha-1), and two levels of potassium (20 and 40 kg ha-1) in a factorial randomized block design.ResultsThe fertilizer application had an immense effect on growth attributes, root yield attributes, dry root yield, and essential oil yield over control. The treatment combination N120, P60, and K40had the largest effect on the plant height, number of leaves per plant, leaf length and width, root length and diameter, dry matter per plant, dry root yield, and essential oil yield. However, the results were at par with the treatment comprising N90, P40, and K20. Dry root yield increased by 108.9%, and essential oil yield increased by 210.3% with fertilizer applications over unfertilized plots. The regression curve shows an increasing trend in dry root yield till N90, P40, and K20; after that, it nearly stabilized. The heat map showed that applying fertilizer significantly affected the chemical constituents ofS. costusessential oil. Similarly, the plots fertilized with the highest level of NPK recorded the utmost value of available N, P, and K, as compared to unfertilized plots.DiscussionThe results emphasize that for sustainable cultivation ofS. costus, the application of N90, P40, and K20combinations is the most suitable one.