Although father love is vital for the positive growth of the child, there is currently no reliable tool to assess the psychological absence of fathers. Therefore, the current study aims to develop an instrument to measure adolescents’ experiences of father-love absence from a psychological absence perspective. According to the fundamental psychological diathesis assumption, the father-love absence scale (FLAS) was developed based on expert panel discussions. A total of 2592 junior high school student participants were surveyed, and exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) was conducted to determine the items for the formal scale. The results showed that the 18-item FLAS consisted of four factors, which were emotional absence (EA), cognitive absence (CA), behavioral absence (BA), and volitional absence (VA). In conclusion, the FLAS demonstrated satisfactory reliability and validity, and this scale is a valuable tool for assessing father-love absence.