﻿AbstractA study of ticks on wildlife was carried out in the area of Levice, Bratislava, Stupava, and Vrbovce (south-western Slovakia) during 2021 and 2022. Overall, 512 ticks were collected from 51 individuals of six wild mammalian species. Eight tick species were identified, namelyDermacentorreticulatus,D.marginatus,Haemaphysalisinermis,H.concinna,Ixodesricinus,I.hexagonus, and twoIxodesspp.Ixodeshexagonuswere collected from northern white-breasted hedgehogs (Erinaceusroumanicus), females belonging toIxodesspp. were collected from red fox (Vulpesvulpes) and nymphs from European badger (Melesmeles).Ixodeshexagonusand theIxodesspp. were identified morphologically and molecularly based on sequences of fragments of two mitochondrial genes,COIand 16S rRNA. Molecular analysis ofIxodesspp. confirmed the identity ofIxodeskaiseriArthur, 1957 andI.canisuga(Johnston, 1849). Sequence analyses show that theI.kaiseriisolate from Slovakia is identical toI.kaiseriisolates from Romania, Poland, Germany, Turkey, and Croatia. We demonstrate for the first time the presence ofI.kaiseriin Slovakia using both morphological and molecular methods.