Cryopreservation allows strains to be stored, eliminating genetic drift and maintenance costs. Existing cryopreservation methods for the economically-important entomopathogenic nematodeSteinernema carpocapsaeinvolve multiple incubation and filtration steps to precondition the animals. The standard protocol for freezing the model organismCaenorhabditiselegansin buffer is simpler, and a recentC. elegansdry-freezing protocol allows stocks to survive multiple freeze-thaws, a possibility during a power failure. Here we report the efficacy ofC. eleganscryopreservation protocols adapted forS. carpocapsae. We show that dry freezing with disaccharides, but not glycerol-based or trehalose-DMSO-based freezing buffer, allows reliable recovery of infective juveniles.