Microbe entry through catheter ports can lead to biofilm accumulation and complications from catheter-related bloodstream infection and ultimately require antimicrobial treatment and catheter replacement. Although strides have been made with microbial prevention by applying standardized antiseptic techniques during catheter implantation, both bacterial and fungal microbes can present health risks to already sick individuals. To reduce microbial adhesion, murine and human catheters were coated with polyurethane and auranofin using a dip coating method and compared to non-coated materials. Upon passage of fluid through the coated materialin vitro, flow dynamics were not impacted. The unique antimicrobial properties of the coating material auranofin has shown inhibitory activity against bacteria such asStaphylococcus aureusand fungi such asCandida albicans. Auranofin coating on catheters at 10mg/mL reducedC.albicansaccumulationin vitrofrom 2.0 x 108to 7.8 x 105CFU for mouse catheters and from 1.6 x 107to 2.8 x 106for human catheters, showing an impact to mature biofilms. Assessment of a dual microbe biofilm on auranofin-coated catheters resulted in a 2-log reduction inS.aureusand a 3-log reduction inC.albicanscompared to uncoated catheters.In vivoassessment in a murine subcutaneous model demonstrated that catheters coated with 10 mg/mL auranofin reduced independentS.aureusandC.albicansaccumulation by 4-log and 1-log, respectively, compared to non-coated catheters. In conclusion, the auranofin-coated catheters demonstrate proficiency at inhibiting multiple pathogens by decreasingS.aureusandC.albicansbiofilm accumulation.