TheMagnaporthe oryzae Triticum(MoT) pathotype is the causal agent of wheat blast, which has caused significant economic losses and threatens wheat production in South America, Asia, and Africa. Three bacterial strains from rice and wheat seeds (B. subtilisBTS-3,B. velezensisBTS-4, andB. velezensisBTLK6A) were used to explore the antifungal effects of volatile organic compounds (VOCs) ofBacillusspp. as a potential biocontrol mechanism against MoT. All bacterial treatments significantly inhibited both the mycelial growth and sporulation of MoT in vitro. We found that this inhibition was caused byBacillusVOCs in a dose-dependent manner. In addition, biocontrol assays using detached wheat leaves infected with MoT showed reduced leaf lesions and sporulation compared to the untreated control. VOCs fromB. velezensisBTS-4 alone or a consortium (mixture ofB. subtilisBTS-3,B. velezensisBTS-4, andB. velezensisBTLK6A) of treatments consistently suppressed MoT in vitro and in vivo. Compared to the untreated control, VOCs from BTS-4 and theBacillusconsortium reduced MoT lesions in vivo by 85% and 81.25%, respectively. A total of thirty-nine VOCs (from nine different VOC groups) from fourBacillustreatments were identified by gas chromatography–mass spectrometry (GC–MS), of which 11 were produced in allBacillustreatments. Alcohols, fatty acids, ketones, aldehydes, and S-containing compounds were detected in all four bacterial treatments. In vitro assays using pure VOCs revealed that hexanoic acid, 2-methylbutanoic acid, and phenylethyl alcohol are potential VOCs emitted byBacillusspp. that are suppressive for MoT. The minimum inhibitory concentrations for MoT sporulation were 250 mM for phenylethyl alcohol and 500 mM for 2-methylbutanoic acid and hexanoic acid. Therefore, our results indicate that VOCs fromBacillusspp. are effective compounds to suppress the growth and sporulation of MoT. Understanding the MoT sporulation reduction mechanisms exerted byBacillusVOCs may provide novel options to manage the further spread of wheat blast by spores.