This study explores how spirituality is integrated into practice across the different Australian health professions. Utilising the Joanna Briggs Institute’s (JBI) protocol, six databases were searched, and sixty-seven articles were finally included. To present the findings, a qualitative synthesis was used. ‘Meaning’ and ‘purpose in life’ were found to be key to many spirituality definitions. The most frequently reported approach for Australian health professionals (HPs) in asking about client spirituality was using one or two questions within a comprehensive assessment. Major facilitators included a holistic care approach and prior training, whereas a key barrier was a lack of time.Supplementary InformationThe online version contains supplementary material available at 10.1007/s10943-023-01840-5.