ObjectiveTo use genomic analysis to identifyAcinetobacterspp. and to explore the distribution characteristics of ß-lactamase oxallicinases (blaOXA) amongAcinetobacterspecies globally.MethodsGenomes of globalAcinetobacterspp. were downloaded from GenBank using Aspera batch. After quality check using CheckM and QUAST software, the genomes were annotated using Prokka software to investigate the distribution ofblaOXAs acrossAcinetobacterspp.; a phylogenetic tree was constructed to explore the evolutionary relationship among theblaOXA genes inAcinetobacterspp. Average-nucleotide identification (ANI) was performed to re-type theAcinetobacterspp. BLASTN comparison analysis was implemented to determine the sequence type (ST) ofAcinetobacter baumanniistrain.ResultsA total of 7,853 genomes were downloaded, of which only 6,639 were left for further analysis after quality check. Among them, 282blaOXA variants were identified from the genomes of 5,893Acinetobacterspp.;blaOXA-23 (n= 3,168, 53.8%) andblaOXA-66 (2,630, 44.6%) were the most frequentblaOXAs, accounting for 52.6% (3,489/6639), and the co-carriage ofblaOXA-23 andblaOXA-66 was seen in 2223 (37.7%) strains. The 282blaOXA variants were divided into 27 clusters according to the phylogenetic tree. The biggest clade wasblaOXA-51-family carbapenem-hydrolyzing enzymes composed of 108blaOXA variants. Overall, 4,923A.baumanniiwere identified out of the 6,639Acinetobacterspp. strains and 291 distinct STs were identified among the 4,904blaOXA-carryingA.baumannii. The most prevalent ST was ST2 (n= 3,023, 61.6%) followed by ST1 (n= 228, 4.6%).ConclusionOXA-like carbapenemases were the mainblaOXA-type β-lactamase spread widely acrossAcinetobacterspp. BothblaOXA-23 andblaOXA-66 were the predominantblaOXAs, among allA.baumanniistrains, with ST2 (belonging to CC2) being the main clone disseminated globally.