Pollution by lead (Pb) is an environmental and health threat due to the severity of its toxicity. Microbial bioremediation is an eco-friendly technique used to remediate contaminated soils. This present study was used to evaluate the effect of two bacterial strains isolated and identified from Bizerte lagoon:Cupriavidus metalliduransLBJ (C.metalliduransLBJ) andPseudomonas stutzeriLBR (P.stutzeriLBR) on the rate of depollution of soil contaminated with Pb from Tunisia. To determine this effect, sterile and non-sterile soil was bioaugmented byP.stutzeriLBR andC.metalliduransLBJ strains individually and in a mixture for 25 days at 30°C. Results showed that the bioaugmentation of the non-sterile soil by the mixture ofP.stutzeriLBR andC.metalliduransLBJ strains gave the best rate of reduction of Pb of 71.02%, compared to a rate of 58.07% and 46.47% respectively for bioaugmentation by the bacterial strains individually. In the case of the sterile soil, results showed that the reduction rate of lead was in the order of 66.96% in the case of the mixture of the two bacterial strains compared with 55.66% and 41.86% respectively for the addition of the two strains individually. These results are confirmed by analysis of the leachate from the sterile and non-sterile soil which showed an increase in the mobility and bioavailability of Pb in soil. These promising results offer another perspective for a soil bioremediation bioprocess applying bacterial bioremediation.