This study was conducted to evaluate the ability of some fungal culture filtrate, as biocontrol agents against okra wilt caused byFusariumsolani. andMeloidogynejavanica. In the present study, fungal culture filtrates (FCFs) ofAspergillusterreus(1),Aspergillusterreus(2),Penicilliumchrysogenum,andTrichodermaspp. were tested againstM.javanicain vitro.The effects ofP.chrysogenumandTrichodermaspp. (FCFs) in controlling root-rot fungi and root-knot nematode disease complex on okra plants were studied under greenhouse conditions (In vivo). In vitro experiment, the results revealed cumulative rate of J2s mortality ofM.javanicareached to 97.67 and 95% byP.chrysogenumandTrichodermaspp., respectively, after 72 h. incubation. Additionally,Trichodermaspp exhibited the most effective inhibitory activity against the pathogen's radial growth, with a percentage of 68%.P.chrysogenumranked second with 53.88%, whileA.terreus(2) demonstrated the weakest inhibitory effect of 24.11%. T6 [Nematode infection (M.javanica) + Fungus infection (F.solani) + Overflowed with fungal culture filtrate (P.chrysogenum)] and T8 [Nematode infection (M.javanica) + Fungus infection (F.solani) + spray with fungal culture filtrate (P.chrysogenum)] had the greatest effects on nematode galling indices on okra roots and substantially reduced the reproductive factors in the greenhouse (In vivo experiment). T6 was the best treatment to decrease disease severity, as reached (28%) relatively. On the other hand, T12 [(Fungus infection (F.solani) + (Dovex 50% fungicide with irrigation water)] recorded the lowest disease severity reaching (8%) relatively. The results showed that nematode infection or fungus infection or both decreased all studied anatomical characteristics of okra root, stem, and leaves. We concluded from this study that root-knot nematode and root-rot fungi were reduced by using fungal culture filtrates and could improve plant growth.