Salmonellais one of the most important foodborne zoonotic pathogens, causing global morbidity and mortality in both humans and animals. Due to the extensive use of antimicrobials in food-producing animals, the antimicrobial resistance ofSalmonellahas attracted increasing attention globally. There have been many reports concerning the antimicrobial resistance ofSalmonellafrom food-producing animals, meats and the environment. However, few studies onSalmonellafrom food-producing animals have been reported in Chongqing municipality, China. The aim of the present study was to determine the prevalence, serovar diversity, sequence types, and antimicrobial resistance ofSalmonellaisolated from livestock and poultry in Chongqing. Meanwhile, we also want to know the presence of β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes and quinolone resistance-determining region (QRDR) mutations ofSalmonellaisolates. A total of 129Salmonellastrains were recovered from 2,500 fecal samples at 41 farms from pigs, goats, beef cattle, rabbits, chickens, and ducks. Fourteen serovars were identified, withS.Agona andS.Derby being the dominant serovars. The 129 isolates had high resistance to doxycycline (87.6%), ampicillin (80.6%), tetracycline (79.8%), trimethoprim (77.5%), florfenicol (76.7%) chloramphenicol (72.9%), and trimethoprim-sulfamethoxazole (71.3%), but were susceptible to cefepime. A total of 114 (88.4%) isolates showed multidrug resistant phenotypes. The prevalence of β-lactamase genes inSalmonellaisolates was 89.9% (116/129), and among these isolates, 107 (82.9%) harboredblaTEM, followed byblaOXA(26, 20.2%),blaCTX-M(8, 6.2%), andblaCMY(3, 2.3%). In addition,qnrB,qnrD,qnrS,oqxA,oqxB, andaac(6′)-Ib-crwere detected in 11, 2, 34, 34, 43, and 72 PMQR-producing isolates, respectively. Moreover, QRDR mutations were very common in PMQR-positiveSalmonellaisolates (97.2%, 70/72) with mutation(s) inparCor combinative mutations ingyrAandparC. More significantly, 32 extended spectrum beta-lactamase (ESBL)-producing isolates were identified, and 62.5% of them were found to harbor one to four PMQR genes. Furthermore, 11 sequence types were identified from the isolates, and most of ESBL-producing isolates were attributed to ST34 (15.6%) and ST40 (62.5%). The coexistence of PMQR genes with β-lactamase genes and the extensive mutations in QRDR present inSalmonellaisolates from food-producing animals suggest a potential threat to public health. Reasonable utilization and strict control strategies for antimicrobials in animal husbandry and animal treatment are necessary to reduce the emergence and dissemination of drug-resistantSalmonellaisolates.