The red porgy (Pagrus pagrus) and the common dentex (Dentex dentex) are Sparidae species of high commercial value, traded in the Greek market. In some cases, fish species identification from Greek fisheries is difficult for the consumer due to the strong morphological similarities with their imported counterparts or closely related species such asPagrus major,Pagrus caeroleustictus,Dentex gibbosusandPagellus erythrinus, especially when specimens are frozen, filleted or cooked. Techniques based on DNA sequencing, such asCOIbarcoding, accurately identify species substitution incidents; however, they are time consuming and expensive. In this study, regions of mtDNA were analyzed with RFLPs, multiplex PCR and HRM in order to develop a rapid method for species identification within the Sparidae family. HRM analysis of a 113 bp region ofcytband/or a 156 bp region of16scould discriminate raw or cooked samples ofP. pagrusandD. dentexfrom the aforementioned closely related species andP. pagrusspecimens sampled in the Mediterranean Sea when compared to those fished in the eastern Atlantic. HRM analysis exhibited high accuracy and repeatability, revealing incidents of mislabeling. Multiple samples can be analyzed within three hours, rendering this method a useful tool in fish fraud monitoring.