Changing the level of pollution in the urban environment is one of the consequences of Covid-19. Litter are one of the most important urban pollutants affected by the Covid-19 pandemic. In this research, the pollution level of urban areas during the Covid-19 pandemic was investigated by studying the urban environment. To this end, the protocol of observation and counting was used and litter were studied in two groups including common litter and Covid-19 related litter in Yasuj, Iran. The results were interpreted based on the clean environment index (CEI). The time of observation was selected based on the peak of the disease and the decline in the incidence rate. The results showed that on average, at the peak of the disease, the density of the litter was reduced by 19% compared to the low lockdown related to Covid-19. The CEI on average was 4.76 at the peak of the disease that was interpreted in the clean status, while the CEI on average was 5.94 at the low lockdown related to Covid-19 so interpreted in the moderate status. Among urban land uses, recreational areas with a difference of more than 60% showed the greatest impact caused by Covid-19, while in commercial areas this difference was less than 3%. The effect of Covid-19 related litter on the calculated index was 73% in the worst case and 0.8% in the lowest case. Although Covid-19 decreased the number of litter in urban areas, the emergence of Covid-19 lockdown related litter was a cause for concern and led to increasing the CEI.