Fish skin is a biological material with high flexibility and compliance and can provide good mechanical protection against sharp punctures. This unusual structural function makes fish skin a potential biomimetic design model for flexible, protective, and locomotory systems. In this work, tensile fracture tests, bending tests, and calculation analyses were conducted to study the toughening mechanism of sturgeon fish skin, the bending response of the whole Chinese sturgeon, and the effect of bony plates on the flexural stiffness of the fish body. Morphological observations showed some placoid scales with drag-reduction functions on the skin surface of the Chinese sturgeon. The mechanical tests revealed that the sturgeon fish skin displayed good fracture toughness. Moreover, flexural stiffness decreased gradually from the anterior region to the posterior region of the fish body, which means that the posterior region (near the tail) had higher flexibility. Under large bending deformation, the bony plates had a specific inhibition effect on the bending deformation of the fish body, especially in the posterior region of the fish body. Furthermore, the test results of the dermis-cut samples showed that the sturgeon fish skin had a significant impact on flexural stiffness, and the fish skin could act as an external tendon to promote effective swimming motion.