Energy harvesting (EH) sources require the tracking of their maximum power point (MPP) to ensure that maximum energy is captured. This tracking process, performed by an MPP tracker (MPPT), is performed by periodically measuring the EH transducer’s output at a given sampling rate. The harvested power as a function of the sampling parameters has been analyzed in a few works, but the power gain achieved with respect to the case of a much slower sampling rate than the EH source’s frequency has not been assessed so far. In this work, simple expressions are obtained that predict this gain assuming a Thévenin equivalent for the EH transducer. It is shown that the power gain depends on the relationship between the square of AC to DC open circuit voltage of the EH transducer. On the other hand, it is proven that harvested power increases, using a suitable constant signal for the MPP voltage instead of tracking the MPP at a low sampling rate. Experimental results confirmed the theoretical predictions. First, a function generator with a series resistor of 1 kΩ was used, emulating a generic Thévenin equivalent EH. Three waveform types were used (sinus, square, and triangular) with a DC voltage of 2.5 V and AC rms voltage of 0.83 V. A commercial MPPT with a fixed sampling rate of 3 Hz was used and the frequency of the waveforms was changed from 50 mHz to 50 Hz, thus effectively emulating different sampling rates. Experimental power gains of 11.1%, 20.7%, and 7.43% were, respectively, achieved for the sinus, square, and triangular waves, mainly agreeing with the theoretical predicted ones. Then, experimental tests were carried out with a wave energy converter (WEC) embedded into a drifter and attached to a linear shaker, with a sinus excitation frequency of 2 Hz and peak-to-peak amplitude of 0.4 g, in order to emulate the drifter’s movement under a sea environment. The WEC provided a sinus-like waveform. In this case, another commercial MPPT with a sampling period of 16 s was used for generating a slow sampling rate, whereas a custom MPPT with a sampling rate of 60 Hz was used for generating a high sampling rate. A power gain around 20% was achieved in this case, also agreeing with the predicted gain.