The influence of heat treatment processes on microstructure, tensile and tribological properties of Ti6Al4V alloy was investigated. The specimens were heated for 30 min at 925 °C and then cooled at various rates by water quenching, air cooling, and furnace cooling. After that, the samples were aged for four hours at 600 °C. Three phases make up the microstructure: primary α-phase (αp), secondary α-phase (αs), and retained β-phase (βr). Cooling in the air and water followed by aging (AC + Aging and WQ + Aging) resulted, αs-phase precipitating inside βr-phase. The highest hardness of 35 HRC was recorded for WQ + Aging specimen due to existence of a high amount of βr-phase and precipitation of αs-phase. On the other hand, the lowest hardness of 26 HRC was obtained for the FC specimen. AC specimen achieved the highest elongation value of 14%. However, WQ + Aging specimen exhibited the highest ultimate tensile strength of 1028 MPa. For WQ + Aging and AC + Aging specimens, the ideal balance of strength and elongation was discovered. The wear resistance of solution-treated specimens was significantly improved by the aging process and 125% improvement could be achieved in WQ compared to WQ + Aging specimens.