AbstractThis research focuses on macrofungi in Baotianman Biosphere Reserve and their relationships with plant ecosystems. The findings demonstrate the reserve’s macrofungal resources. The study collected 832 specimens, among which 351 macrofungi species were identified, belonging to six classes, 19 orders, 54 familiae, and 124 genera, and one new species ofAbortiporuswas found. Among them, 11 familiae with a total of 231 species were dominated, accounting for 20.37% of the total number of familiae and 65.81% of the total number of species; 14 genera with a total of 147 species were dominated, accounting for about 11.38% the total number of genera and 41.88% of the total number of species. The richness of macrofungi at the species level was considerably different across the four vegetation types in the reserve, showing that the vegetation types had a bigger influence on macrofungi. In the evaluation of macrofungal resources, a total of 196 species of edible fungi, 121 species of medicinal fungi, 52 species of poisonous fungi, and 37 species of macrofungi with unclear economic value were counted.Abortiporus baotianmanensisis a new species of podoscyphaceae in the genusAbortiporus. The new species display the reserve’s richness. Next, the project seeks to generate and conserve macrofungal resources.