Exposure to ultraviolet (UV) radiation is one of the major factors that causes skin aging, erythema, sunburns, and skin cancer. This study aimed to select probiotic bacterial isolates able to produce high yield of hyaluronic acid (HA) to be employed for skin photoprotection and other possible biological applications. The selected isolates K11 and St3 were able to produce the highest yields of HA 4.8 and 4.4 mg/ml, respectively. Both isolates were identified asEnterococcus duransstrain K11 andLactiplantibacillus plantarumstrain St3 using 16S rRNA gene sequencing. The antioxidant activity of HA produced byE. duransstrain K11 andL. plantarumstrain St3 was (65.4 0.2%) and (66.6 0.1%), respectively. The viability of UVB-irradiated keratinocytes pre-treated with HA produced byE. duransstrain K11 andL. plantarumstrain St3 was 91.3 and 91.4%, respectively, compared with the control. While the viability of UVB-irradiated keratinocytes post-treated with HA produced byE. duransstrain K11 andL. plantarumstrain St3 was 86 and 88.5%, respectively. To the best of our knowledge, this is the first recordation of HA production byEnterococcus duransandLactiplantibacillus plantarumwhich revealed a significant radioprotection of the human keratinocytes against UVB radiation.Supplementary InformationThe online version contains supplementary material available at 10.1007/s00284-023-03377-y.