Background and Aim:Raw goat milk is a highly nutritious dairy product and a suitable medium for the growth of antimicrobial-resistantStaphylococcus aureus, the leading cause of subclinical mastitis. This study aimed to investigate the resistance status ofS. aureuswhich isolated from goat milk associated with subclinical mastitis cases in Siliragung Subdistrict, Banyuwangi District, East Java, Indonesia.Materials and Methods:TheS. aureusisolates were recovered from 258 raw goat milk samples from seven different dairy goat farms. Preliminary screening of subclinical mastitis using the California mastitis test, then samples with score +3 and +4 were taken for further isolation and identification, followed by a biochemical test to determine theS. aureus. Moreover, the bacteria susceptibility test against several antimicrobials was done using the disk diffusion method.Results:Based on our findings, a total of 66 (25.58%) raw goat milk samples were tested positive forS. aureus, of which 36.36% were identified as multidrug-resistant. Moreover,S. aureuswere also identified as resistant to penicillin (81.82%), ampicillin (65.15%), erythromycin (50.52%), and gentamicin (36.09%).Conclusion:The prevalence ofS. aureusisolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, Banyuwangi District, Indonesia, was recorded at 25.58%. Moreover, 36.36% ofS. aureusisolates were categorized as resistant to three or more classes of antibiotics. The biosafety and biosecurity procedures during the milking process should be strengthened in dairy goat farms to prevent the transmission of antimicrobial resistance among animals, humans, and environments.