Simple SummaryIn recent years, wild boars’ populations have been expanding both in rural and urban areas in Europe. Since these animals are placed in the interface of domestic and sylvatic cycle for zoonotic and animal-specific parasites, the aim of this study was to evaluate the serological prevalence ofToxoplasma gondii,Neospora caninum, andSarcoptes scabieivar.suisin wild boars hunted in an anthropized area in Italy. The results confirmed the possible risk ofT. gondiiinfection for humans from consumption of meat and meat products from wild boars. Besides, their role as intermediate hosts forN. caninumis relevant due to the high presence of dairy cattle farms in the area. The usefulness of serological analysis to estimateS. scabieiinfestation in wild boars is confirmed to evaluate the sanitary risk for livestock and humans in the area. In conclusion, the constitution of surveillance plans by standardized methods should be emphasized also to promote the awareness among hunters, wildlife professionals, and consumers on the circulation of parasites of this wild species with impact both on human and animal health.AbstractDue to the increasing expansion into urban and rural areas, wild boars represent a potential source of infection with zoonotic and animal-specific parasites for both humans and animals. Therefore, the aim of this study was to investigate the serological prevalence toToxoplasma gondii,Neospora caninum, andSarcoptes scabieivar.suisin blood samples from wild boars (Sus scrofa) hunted in an anthropized area in Italy. Enzyme-linked immunosorbent assay (ELISA) tests were used to detect antibodies anti-T. gondiiand anti-S. scabieiand an immunofluorescence antibody test (IFAT) for antibodies anti-N. caninum. 81 out of 128 wild boars (P = 63.3%) resulted positive for at least one of the three parasites. 68 of them were seropositive toT. gondii(P = 53.1%) and 14 toN. caninum(P = 10.9%). 9 wild boars resulted seropositive toS. scabieivar.suis(P = 7.0%). Sampling season was the only significant risk factor related toS. scabieivar.suisseroprevalence (OR = 7.8). The high occurrence ofT. gondiisupports the role of this species as a source of infection for other animals and humans. Furthermore, the serological prevalence ofN. caninumandS. scabieivar.suisin wild boars from the study area characterized by the presence of numerous dairy cattle and pig farms is relevant to demonstrate its suitability for the circulation of these parasites both in domestic and wild species.