BackgroundOdour-baited traps are useful for vector surveillance and control. However, most existing traps have shown inconsistent recapture rates across different mosquito species, necessitating the need for more effective and efficient traps. The MTego trap with integrated thermal stimuli has been developed as an alternative trap. This study was undertaken to determine and compare the efficacy of the MTego trap to that of the Biogents (BG) modular BG-Pro (BGP) trap for sampling different mosquito species in a semi-field system.MethodsFully balanced Latin square design experiments (no-choice and dual choice) were conducted in semi-field chambers using laboratory-reared femaleAnopheles gambiaesensu stricto,Anopheles funestus,Anopheles arabiensis,Culex quinquefasciatusandAedes aegypti. There were 16 replicates, and 50 mosquitoes of each species were released in each chamber per replicate. The evaluated traps were as follows: the MTego trap baited with PM6 (MT-PM6), the MTego trap baited with BG-Lure (BGL) (MT-BGL), and the BGP trap baited with BG-Lure (BGP-BGL).ResultsIn the no-choice test, the MT-BGL and BGP-BGL traps captured a similar proportion ofAn. gambiae(31% vs 29%,P-value = 0.519) andAn. funestus(32% vs 33%,P= 0.520). The MT-PM6 and BGP-BGL traps showed no significant difference in capturingAe. aegypti(33% vs 31%,P= 0.324). However, the BGP-BGL caught moreAn. arabiensisandCx. quinquefasciatusmosquitoes than the other traps (P< 0.0001). In the dual-choice test of MT-PM6 vs BGP-BGL, similar proportions ofAn. funestus(25% vs 27%,P= 0.473) andAe. aegypti(29% vs 25%,P= 0.264) were captured in the traps, while the BGP-BGL captured moreAn. gambiae,An. arabiensisandCx. quinquefasciatusmosquitoes than the MT-PM6 (P< 0.0001).ConclusionsThis study demonstrated that the MTego trap has potential as a tool that can be used interchangeably with the BGP trap for sampling anthropophilic mosquitoes including African malaria vectorsAn. gambiaeandAn. funestusand the principal arbovirus vectorAe. aegypti.Graphical abstract