Highlights•The fluorescence or LFS assay based on RT-RPA-Cas12a is a point-of-care diagnosis method.•The fluorescence or LFS assay based on RT-RPA-Cas12a detection can be completed within 50 min with high specificity.•The RT-RPA-Cas12a mediated fluorescence or LFS assay demonstrates high sensitivity (0.1 or 0.5 copy per microliter).