IntroductionCandidais one of the rising primary causes of infections connected with health care. However, their distribution and susceptibility patterns vary widely amongst different regions.MethodThe study was carried out to retrospectively analyze the distribution ofCandidain various clinical samples, their species types and susceptibility, in a tertiary care hospital, in India for 4 years using the Vitek-2 database.ResultsCandidainfection was identified in 751 clinical samples, and the major source of infection was found to be urine samples accounting for about 58.32%. A total of 18 differentCandidaspecies were recorded. Non-albicansCandida(NAC) 73.64% (n= 553) predominatedCandida albicans26.36% (n= 198).Candida tropicaliswas found to be identified at a higher frequency followed byC. albicans,Candida glabrataandCandida parapsilosis.Candida tropicaliswas the only species which were recovered from bile;Candida pelliculosawas recorded merely from blood andCandida lipolyticafrom urine and blood and not in any other samples. In vaginal swabs,C. albicansaccounted for 63.64% (n= 14) compared to NAC 36.36% (n= 8). The susceptibility test revealed that 75.44% (n= 559) isolates were susceptible and 24.56% (n= 182) were resistant to one or more drugs tested. Major resistance was exhibited to flucytosine byC. tropicalis77.46% (n= 55) compared toC. albicans11.27% (n= 8). Apart fromC. albicans, NAC-C. tropicalis,C. glabrata and Candida kruseishowed resistance to echinocandins, andCandida haemuloniito amphotericin-B.ConclusionThe knowledge of the incidence, resistance and emergence of different species might guide clinicians to select an appropriate antifungal therapy and plan effective strategies to control invasive and systemicCandidainfections.