We detectedLeishmaniaRNA virus 1 (LRV1) in 11 isolates ofLeishmania (Viannia) panamensiscollected during 2014–2019 from patients from different geographic areas in Panama. The distribution suggested a spread of LRV1 inL. (V.) panamensisparasites. We found no association between LRV1 and an increase in clinical pathology.