Cardio-pulmonary parasites, such asAngiostrongylus vasorum,Crenosoma vulpis, andEucoleus aerophilus, pose a significant concern on account of pulmonary and cardiac problems they induce in dogs. While the red fox is known to be a key reservoir host forA. vasorumand may also play a role in transmittingC. vulpisandE. aerophilus, there has been no recent research on these parasites in foxes from Sardinia, with the most current studies dating back to 1986. A survey was conducted on red foxes in Sardinia, where a total of 51 foxes were collected, necropsied, and examined for adult worms in their hearts and lungs. The worms were identified using morphometric analysis and molecular methods. The results showed a 54.9% overall prevalence at dissection: 45.1% of the foxes were positive forE. aerophilus, 17.6% forC. vulpis, and 13.7% forA. vasorum. The molecular analyses validated the morphological characterization. In comparison to previous research, which found 13 out of 85 foxes to be positive forA. vasorumwith a prevalence rate of 15.3% and 1 forE. aerophiluswith a prevalence of 1.2%, this study showed an increased prevalence ofE. aerophilusandC. vulpis, and a decrease in the prevalence ofA. vasorum. These results indicate that the red foxes in Sardinia represent a reservoir host for cardio-pulmonary nematodes and it should be considered in the differential diagnosis of respiratory distress syndrome in dogs.