The present study aimed to test intestinal scrapings of the Northern Goshawk (Accipiter gentilis) and the Eurasian Sparrowhawk (Accipiter nisus) from Lithuania forS.calchasiand otherSarcocystisspecies characterised by bird–bird life cycles. The protozoan parasiteSarcocystis calchasican cause respiratory and neurological diseases in a variety of birds; however, the distribution of this parasite is not well-examined.Sarcocystisspecies were identified with nested PCR and sequencing of the partialITS1region. Sporocysts and/or sporulated oocysts ofSarcocystisspp. were observed in 16 (100%) Northern Goshawks and 9 (56.3%) Eurasian Sparrowhawks. Four species,S.columbae,S.halieti,S.turdusi, andS.wobeseri, were confirmed in the Eurasian Sparrowhawk. Apart from the latter four species,S.calchasi,S.cornixi,S.kutkienae, andS.lariwere established in the Northern Goshawk. A higher prevalence ofSarcocystisspp. and species richness in Northern Goshawks is associated with the differences in the diet of two examinedAccipiterspecies. This study is the first report ofS.calchasiin Lithuania. Furthermore, the genetically distinct speciesSarcocystisspp. 23LTAcc, which is most closely related toS.calchasi, was found in three Northern Goshawks.