Understanding the transmission ofSchistosoma hæmatobiumin the Senegal River Delta requires knowledge of the snails serving as intermediate hosts. Accurate identification of both the snails and the infectingSchistosomaspecies is therefore essential. Cercarial emission tests and multi-locus (COX1 and ITS) genetic analysis were performed onBulinus forskaliisnails to confirm their susceptibility toS.hæmatobiuminfection. A total of 55Bulinus forskalii, adequately identified by MALDI-TOF mass spectrometry, were assessed. Cercarial shedding and RT-PCR assays detected 13 (23.6%) and 17 (31.0%), respectively,Bulinus forskaliisnails parasitized byS.hæmatobiumcomplex fluke. Nucleotide sequence analysis identifiedS.hæmatobiumin 6 (11.0%) using COX1 and 3 (5.5%) using ITS2, andS.bovisin 3 (5.5%) using COX1 and 3 (5.5%) using ITS2. This result is the first report of infection ofBulinus forskaliibyS.hæmatobiumcomplex parasites in Senegal using innovative and more accurate identification methods to discriminate this snail and characterize its infection by S.hæmatobium.