Selenium (Se) hyperaccumulators are a unique group of plants that can accumulate this element in their aerial parts at concentrations exceeding 100 mg kgDW−1. These plants actively search for Se in the soil, a phenomenon known asroot foraging, reported to date only by few studies. In this study, the effect of localized Se enrichment, in the form of selenite and selenate, was investigated on the root architecture of two Se-hyperaccumulators (Stanleya pinnataandAstragalus bisulcatus) and two non-accumulators (Brassica junceaandMedicago sativa). Rhizoboxes were divided into two halves: one half was filled with control soil while the other with selenate or selenite (30 mg kgDW−1) spiked soil. Seedling were transferred into the interface of the two soils and allowed to grow for three weeks under controlled light and temperature conditions.Staneya pinnataexhibited equal root density in both halves of the rhizobox when grown in control/control and selenite/control soil treatments. However, in the presence of selenate,S. pinnatadeveloped 76% of the roots towards the selenate-enriched half, indicating an active root foraging. In contrast,A. bisulcatusand the non-accumulatorsB. junceaandM. sativadid not show any preferential distribution of roots. This study revealed that onlyS. pinnatashowed the ability to detect and forage for Se when provided as selenate. Non-accumulators did not show any morphological or Se-accumulation difference associated with the presence of Se in soil in either form.