ObjectiveTo evaluate the antitumoral role of γδ TDCcells and αβ TDCcells in an experimental model of breast cancer.MethodsThirty female Balb/c mice were divided into 2 groups: control group (n= 15) and induced-4T1 group (n= 15), in which the mice received 2 × 1054T1 mammary tumor cell line. Following the 28-day experimental period, immune cells were collected from the spleen and analyzed by flow cytometry for comparison of αβ TDC(TCRαβ+CD11c+MHCII+) and γδ TDC(TCRγδ+CD11c+MHCII+) cells regarding surface markers (CD4+and C8+) and cytokines (IFN-γ, TNF-α, IL-12 and IL-17).ResultsA total of 26.53% of γδ TDC- control group (p< 0.0001) - the proportion of αβ TDCwas lower in splenic cells than γδ TDC; however, these 2 cell types were reduced in tumor conditions (p< 0.0001), and the proportion of IFN-γ, TNF-α, IL-12 and IL-17 cytokines produced by γδ TDCwas higher than those produced by αβ TDC, but it decreased under conditions of tumor-related immune system response (p< 0.0001).ConclusionHealthy mice engrafted with malignant cells 4T1 breast tumor presented TDCwith γδ TCR repertoire. These cells express cytotoxic molecules of lymphocytes T, producing anti-tumor proinflammatory cytokines.