ABSTRACTHere, we present the genome sequence of a novelPseudomonas aeruginosabacteriophage called UF_RH1. This lytic phage has a genome size of 42,567 bp and is classified as a member of theSiphoviridaefamily and theSeptimatrevirusgenus. UF_RH1 shares genetic similarities withStenotrophomonasphage vB_SmaS-DLP_2.