In the field of precision livestock farming, many systems have been developed to identify the position of each cow of the herd individually in a specific environment. Challenges still exist in assessing the adequacy of the available systems to monitor individual animals in specific environments, and in the design of new systems. The main purpose of this research was to evaluate the performance of the SEWIO ultrawide-band (UWB) real time location system for the identification and localisation of cows during their activity in the barn through preliminary analyses in laboratory conditions. The objectives included the quantification of the errors performed by the system in laboratory conditions, and the assessment of the suitability of the system for real time monitoring of cows in dairy barns. The position of static and dynamic points was monitored in different experimental set-ups in the laboratory by the use of six anchors. Then, the errors related to a specific movement of the points were computed and statistical analyses were carried out. In detail, the one-way analysis of variance (ANOVA) was applied in order to assess the equality of the errors for each group of points in relation to their positions or typology, i.e., static or dynamic. In the post-hoc analysis, the errors were separated by Tukey’s honestly significant difference atp> 0.05. The results of the research quantify the errors related to a specific movement (i.e., static and dynamic points) and the position of the points (i.e., central area, perimeter of the investigated area). Based on the results, specific information is provided for the installation of the SEWIO in dairy barns as well as the monitoring of the animal behaviour in the resting area and the feeding area of the breeding environment. The SEWIO system could be a valuable support for farmers in herd management and for researchers in the analysis of animal behavioural activities.