Highlights•506 enterococcal phage and prophage genomes were scanned for tail module lysins.•Identified lysins target cell wall, teichoic acids and enterococcal polysaccharide antigen.•Most common identified lysin was endopeptidase in both phage and prophage genomes.•Lytic transglycosylase lysins possess unique motifs and locate in tape measure proteins.•This study provides a rich foundation for phage engineering and TAL recombinant expression.