Chemogenetic Operation of iNTRacellular prOton Levels
(pH-Control)
is a novel substrate-based enzymatic method that enables precise spatiotemporal
control of ultralocal acidification in cultured cell lines and primary
neurons. The genetically encoded biosensor SypHer3s showed that pH-Control
effectively acidifies cytosolic, mitochondrial, and nuclear pH exclusively
in the presence of β-chloro-d-alanine in living cells
in a concentration-dependent manner. The pH-Control approach is promising
for investigating the ultralocal pH imbalance associated with many
diseases.