﻿AbstractWoody oil plants are important economic trees which are widely cultivated and distributed throughout China. Surveys conducted during 2020 and 2021 on several woody oil plantations from five regions of Sichuan Province, China, revealed a high diversity of Botryosphaerialean fungi. The identification of 50 botryosphaeriaceous isolates was carried out based on both morphology and multi-gene phylogenetic analysis of internal transcribed spacer region (ITS), translation elongation factor 1-alpha gene (tef1) and β-tubulin gene (tub2). This allowed the identification of twelve previously knownBotryosphaerialesspecies:Aplosporellaprunicola,A.ginkgonis,Barriopsistectonae,Botryosphaeriadothidea,Bo.fabicerciana,Diplodiamutila,Di.seriata,Dothiorellasarmentorum,Neofusicoccumparvum,Sardiniellaguizhouensis,Sphaeropsiscitrigena, andSp.guizhouensis, and four novel species belonging to the generaDiplodiaandDothiorella, viz.Di.acerigena,Di.pistaciicola,Do.camelliaeandDo.zanthoxyli. The dominant species isolated across the surveyed regions wereBotryosphaeriadothidea,SardiniellaguizhouensisandDiplodiamutila, representing 20%, 14% and 12% of the total isolates, respectively. In addition, most isolates were obtained fromPistaciachinensis(14 isolates), followed byCamelliaoleifera(10 isolates). The present study enhances the understanding ofBotryosphaerialesspecies diversity on woody oil plants in Sichuan Province, China.