Zambia has seen rapid development in aquaculture, and in recent years, the industry has experienced disease outbreaks where fish have increasingly become a potential contributor to emerging bacterial zoonotic diseases. The aim of this study was to identify bacterial pathogens with zoonotic potential in apparently healthy fish and water from their habitat. A total of sixty-three fish were sampled, and fifty-nine water samples were collected from the habitats of these fish. Bacteria were cultured from the internal organs of fish and water, and these were identified through standard bacteriological methods comprising morphological characterization, Gram-staining, and a panel of biochemical tests. The following bacterial pathogens with zoonotic potential were identified at a farm prevalence ofAeromonas(13.2%),Bacillus(2.1%),Clostridium(2.1%),Escherichia coli(0.7%),Klebsiella(6.9%),Lactococcus(2.1%),Listeria(0.7%),Staphylococcus(18.1%), andStreptococcus(0.7). Other bacteria with varying significance as fish pathogens identified includedAcinetobacter(2.1%),Aequorivita(1.4%),Aerococcus(1.4%),Bordetella(2.1%),Carnobacterium(10.4%),Citrobacter(3.5%),Corynebacterium(1.4%),Dermatophilus(1.4%),Enterococcus(2.1%),Flavobacterium(4.2%),Micrococcus(6.9%),Planococcus(1.4%),Proteus(1.4%),Pseudomonas(6.3%),Rhodococcus(1.4%),Shewanella(1.4%),Streptococcus(0.7%), andVagococcus(0.7%). The current study provides baseline information for future reference and the implementation of public health guidelines with regard to potential zoonotic diseases in fish.