Oral probiotic lactic acid bacteria can exhibit antagonistic activities against pathogens associated with diseases in the oral cavity. Therefore, twelve previously isolated oral strains were assessed for antagonistic evaluation against selected oral test microorganismsStreptococcus mutansandCandida albicans. Two separate co-culturing analyses were performed, where all tested strains showed the presence of antagonistic activity and four strains,Limosilactobacillus fermentumN 2, TC 3-11, and NA 2-2, andWeissella confusaNN 1, significantly inhibitedStreptococcus mutansby 3–5 logs. The strains showed antagonistic activity againstCandida albicans, and all exhibited pathogen inhibition by up to 2 logs. Co-aggregation capability was assessed, showing co-aggregative properties with the selected pathogens. Biofilm formation and antibiofilm activity of the tested strains against the oral pathogens were assayed, where the strains showed specificity in self-biofilm formation and well-expressed antibiofilm properties by most of them above 79% and 50% againstStreptococcus mutansandCandida albicans, respectively. The tested LAB strains were assayed by a KMnO4antioxidant bioassay, where most of the native cell-free supernatants exhibited total antioxidant capacity. These results show that five tested strains are promising candidates to be included in new functional probiotic products for oral healthcare.