ABSTRACTPacific Biosciences (PacBio) long-read sequencing was used to generate a chromosome-level genome assembly forYamadazyma tenuisstrain ATCC 10573. The assembly featured 7 chromosomes that matched the electrophoretic karyotype and a 26.5-kb circular mitochondrial genome. The nuclear genome was 10.8 Mb, with a GC content of 43%, and 5,340 predicted genes.