Over the past few years, spatial memory has been studied using virtual-reality-based tasks. Reversal learning has been widely used in spatial orientation tasks for testing, among other things, new learning and flexibility. By means of a reversal-learning protocol, we assessed spatial memory in men and women. A total of sixty participants (half of them women) performed a task that included two phases: during the acquisition phase, participants were asked to find one or three rewarded positions in the virtual room across ten trials. During the reversal phase, the rewarded boxes were moved to a new position and maintained for four trials. The results showed that men and women differed in the reversal phase, with men outperforming women in high demanding conditions. Dissimilarities in several cognitive abilities between both genders are the base of these differences and are discussed.