Campylobacterspp. are considered the most frequent cause of acute gastroenteritis worldwide. However, outside high-income countries, its burden is poorly understood. Limited published data suggest thatCampylobacterprevalence in low- and middle-income countries is high, but their reservoirs and age distribution are different. CulturingCampylobacteris expensive due to laboratory equipment and supplies needed to grow the bacterium (e.g., selective culture media, microaerophilic atmosphere, and a 42°C incubator). These requirements limit the diagnostic capacity of clinical laboratories in many resource-poor regions, leading to significant underdiagnosis and underreporting of isolation of the pathogen. CAMPYAIR, a newly developed selective differential medium, permitsCampylobacterisolation without the need for microaerophilic incubation. The medium is supplemented with antibiotics to allowCampylobacterisolation in complex matrices such as human feces. The present study aims to evaluate the ability of the medium to recoverCampylobacterfrom routine clinical samples. A total of 191 human stool samples were used to compare the ability of CAMPYAIR (aerobic incubation) and a commercialCampylobactermedium (CASA, microaerophilic incubation) to recoverCampylobacter. AllCampylobacterisolates were then identified by MALDI-TOF MS. CAMPYAIR showed sensitivity and specificity values of 87.5% (95% CI 47.4%–99.7%) and 100% (95% CI 98%–100%), respectively. The positive predictive value of CAMPYAIR was 100% and its negative predictive value was 99.5% (95% CI 96.7%–99.9%); Kappa Cohen coefficient was 0.93 (95% CI 0.79–1.0). The high diagnostic performance and low technical requirements of the CAMPYAIR medium could permitCampylobacterculture in countries with limited resources.