﻿AbstractA new species of freshwater mussel belonging to the genusPseudocuneopsis, namelyPseudocuneopsisyangshuoensissp. nov., is diagnosed and described from Guangxi Province, China. This paper provides a detailed morphological description, photograph of the type specimen, and anatomical characteristics along with partial sequences of mitochondrial COI as DNA barcode data for this novel species. The new species can be distinguished from its congeners (PseudocuneopsissichuanensisandPseudocuneopsiscapitata) by shell shape, beak position and surface sculpture. The interspecies genetic distance based on the COI barcode betweenP.yangshuoensissp. nov.andP.sichuanensisis 8%, while it reaches 9% withP.capitata. Therefore, we provide robust morphological and molecular evidence to support the validity of this new species.