Pathogen-related proteins (PRs) are diversified proteins with a low molecular weight implicated in plant response to biotic and abiotic stress as well in regulating different functions in plant maturation. Interestingly, no systematical study has been conducted in durum wheat (Triticum turgidumsubsp.durum). In the present study,12 PR-1 genesencoding a CAP superfamily domain were identified in the genome ofTriticum turgidumsubsp.durum, which is an important cereal, using in silico approaches. Additionally, phylogenetic analysis showed that thePR-1genes were classified into three groups based on their isoelectric point and the conserved motif domain. Moreover, our analysis showed that most of the TdPR-1 proteins presented an N-terminal signal peptide. Expression patterns analysis showed that thePR-1gene family presented temporal and spatial specificity and was induced by different abiotic stresses. This is the first report describing the genome-scale analysis of the durum wheatPR-1gene family, and these data will help further study the roles ofPR-1genes during stress responses, leading to crop improvement.