Wood Decay Fungi (WDF) are fungi specialized in degrading wood. An interesting perspective is their use as a source of Novel Foods or food ingredients. Here, for the first time, the metabolite profiling of hydroalcoholic and organic extracts fromA. biennis,F. iberica,S. hirsutummycelia was investigated by NMR methodology. Amino acids (alanine, arginine, asparagine, aspartate, betaine, GABA, glutamate, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, tyrosine, valine), sugars (galactose, glucose, maltose, trehalose, mannitol), organic acids (acetate, citrate, formate, fumarate, lactate, malate, succinate), adenosine, choline, uracil and uridine were identified and quantified in the hydroalcoholic extracts, whereas the1H spectra of organic extracts showed the presence of saturated, mono-unsaturated and di-unsaturated fatty chains, ergosterol,1,2-diacyl-sn-glycero-3-phosphatidylethanolamine, and 1,2-diacyl-sasglycero-3-phosphatidylcholine.A. biennisextracts showed the highest amino acid concentration. Some compounds were detected only in specific species: betaine and mannitol inS. hirsutum, maltose inA. biennis, galactose inF. iberica, GABA inF. ibericaandS. hirsutum, and acetate inA. biennisandS. hirsutum.S. hirsutumshowed the highest saturated fatty chain concentration, whereas DUFA reached the highest concentration inA. biennis.A high amount of ergosterol was measured both inA. biennisandF. iberica. The reported results can be useful in the development of WDF-based products with a high nutritional and nutraceutical value.