Humans need to accurately infer the intentions and feelings of others to engage in successful social interaction. However, the application of artificial intelligence technology in Education (AIEd) forms a human–machine collaborative environment which changed the interaction relationship of individuals, it may have an affect on them. This study aimed to explore whether AIEd affects adolescents’ emotional perception. Combined with the actual teaching situation and the result of the questionnaire, 1332 students recruited through random sampling from AI Curriculum Reform Demonstration Schools in Guangzhou participated in this study. Different emotional priming stimulative materials (sentences and situational pictures) were used in the experiments. The task was designed to investigate adolescents’ reaction time to emotional faces (positive, negative). After eliminating blank data and invalid data with response time greater than 150 ms, 977 and 962 valid data were included in the statistical analysis in experiment 1 and experiment 2 respectively. Results show that AIEd has a negative effect on adolescents’ emotional perception. Prior research has focused on theory to the exclusion of practical applications and the psychological impact of AIEd, thus this study makes an innovative contribution in exploring the impact of the application of artificial intelligence technology in education on adolescents’ physical and mental development by using empirical research methods.