We explored the genome of theWolbachiastrain,wEsol, symbiotic with the plant-gall-inducing flyEurosta solidaginiswith the goal of determining ifwEsol contributes to gall induction by its insect host. Gall induction by insects has been hypothesized to involve the secretion of the phytohormones cytokinin and auxin and/or proteinaceous effectors to stimulate cell division and growth in the host plant. We sequenced the metagenome ofE. solidaginisandwEsol and assembled and annotated the genome ofwEsol. ThewEsol genome has an assembled length of 1.66 Mbp and contains 1878 protein-coding genes. ThewEsol genome is replete with proteins encoded by mobile genetic elements and shows evidence of seven different prophages. We also detected evidence of multiple small insertions ofwEsol genes into the genome of the host insect. Our characterization of the genome ofwEsol indicates that it is compromised in the synthesis of dimethylallyl pyrophosphate (DMAPP) and S-adenosyl L-methionine (SAM), which are precursors required for the synthesis of cytokinins and methylthiolated cytokinins.wEsol is also incapable of synthesizing tryptophan, and its genome contains no enzymes in any of the known pathways for the synthesis of indole-3-acetic acid (IAA) from tryptophan.wEsol must steal DMAPP and L-methionine from its host and therefore is unlikely to provide cytokinin and auxin to its insect host for use in gall induction. Furthermore, in spite of its large repertoire of predicted Type IV secreted effector proteins, these effectors are more likely to contribute to the acquisition of nutrients and the manipulation of the host’s cellular environment to contribute to growth and reproduction ofwEsol than to aidE. solidaginisin manipulating its host plant. Combined with earlier work that shows thatwEsol is absent from the salivary glands ofE. solidaginis, our results suggest thatwEsol does not contribute to gall induction by its host.