Highlights•JKP2 is a first isolated phage againstKlebsiellaK-17 capsular serotype.•It produced bulls eye shaped clear lytic plaques due to tail associated depolymerase.•JKP2 remained stable at tested pH (5 to 10) and temperatures (37 to 60 °C).•JKP2 showedK. pneumoniaebiofilm reduction potential.•Based on whole genome sequence analysis it belong to unclassifiedDrulisviruswithin theAutographiviridaefamily.