ABSTRACTWe determined the echinocandin susceptibility andFKS1genotypes of 13 clinical isolates ofCandida auristhat were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novelFKS1mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptibleC. aurisstrains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32×; caspofungin, >64×; micafungin, >64×).