Aminoglycosides are used to treat infections caused by carbapenem-resistantAcinetobacter baumannii(CRAB) strains. However, resistance to aminoglycosides has increased remarkably in the last few years. Here, we aimed to determine the mobile genetic elements (MGEs) associated with resistance to aminoglycosides in the global clone 2 (GC2)A. baumannii. Among the 315A. baumanniiisolates, 97 isolates were identified as GC2, and 52 of GC2 isolates (53.6%) were resistant to all the aminoglycosides tested. The AbGRI3s carryingarmAwere detected in 88 GC2 isolates (90.7%), and of them, 17 isolates (19.3%) carried a new variant of AbGRI3 (AbGRI3ABI221).aphA6was located in TnaphA6of 30 isolates out of 55aphA6-harboring isolates, and 20 isolates were found to harbor TnaphA6on a RepAci6 plasmid. Tn6020carryingaphA1bwas detected in 51 isolates (52.5%), which was located within AbGRI2 resistance islands. The pRAY*carrying theaadBgene was detected in 43 isolates (44.3%), and no isolate was found to contain a class 1 integron harboring this gene. The GC2A. baumanniiisolates contained at least one MGE carrying the aminoglycoside resistance gene, located mostly either in the chromosome within AbGRIs or on the plasmids. Thus, it is likely that these MGEs play a role in the dissemination of aminoglycoside resistance genes in GC2 isolates from Iran.