AbstractFollowing a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on concentrated liquidl‐lysine,l‐lysine monohydrochloride and concentrated liquidl‐lysine monohydrochloride produced by a genetically modified strain ofEscherichia coli(NITE BP‐02917) as nutritional and as sensory (flavouring compound) feed additives for all animal species. In 2022, the FEEDAP Panel issued an opinion on the safety and efficacy of these products. In that assessment, the FEEDAP Panel could not exclude the potential presence of recombinant DNA derived from the genetically modified production organism in the products. The applicant provided supplementary data to exclude the presence of recombinant DNA derived from the production organism in the final products. Based on the new data provided, the FEEDAP Panel concluded that no DNA of the production strainE. coliNITE BP‐02917 was detected in concentrated liquidl‐lysine,l‐lysine monohydrochloride and concentrated liquidl‐lysine monohydrochloride.