The scientific need for standardized, high-quality facial stimuli has driven the creation of several face image databases in recent years. These stimuli are particularly important in facial asymmetry research. However, previous studies have reported facial anthropometric differences across a variety of ethnicities. This highlights the need to investigate whether these differences can also impact the use of face image databases, particularly in facial asymmetry research. In this study, we investigated facial asymmetry-based morphometric differences between the multi-ethnic Chicago Face Database (CFD) and the LACOP Face Database, which is composed of Brazilian subjects. We found reliable differences in facial asymmetry between the two databases, which were related to ethnic groups. Specifically, differences in eye and mouth asymmetry seem to drive these differences. The asymmetry-based morphometric differences among databases and ethnicities found in this study reinforce the necessity of creating multi-ethnic face databases.