Anthracological analyses of charcoal samples retrieved from Pit 16 of Perdigões (Reguengos de Monsaraz, Portugal), a secondary deposition of cremated human remains dated back to the middle of the 3rdmillennium BC, enabled the identification of 7 differenttaxa:Olea europaea,Quercusspp. (evergreen),Pinus pinaster,Fraxinuscf.angustifolia,Arbutus unedo,Cistussp. and Fabaceae. Alltaxaare characteristic of both deciduous and evergreen Mediterranean vegetation, and this data might indicate that the gathering of woods employed for the human cremation/s occurred either on site, or in its vicinity. However, considering both the large distribution of the identifiedtaxaand data about human mobility, it is not possible to conclusively determine the origin of the wood used in the cremation(s). Chemometric analysis were carried out to estimate the absolute burning temperature of woods employed for the human cremation/s. An in-lab charcoal reference collection was created by burning sound wood samples of the three maintaxaidentified from Pit 16,Olea europaeavar.sylvestris,Quercus suber(evergreen type) andPinus pinaster, at temperatures between 350 and 600 °C. The archaeological charcoal samples and the charcoal reference collection were chemically characterized by using mid-infrared (MIR) spectroscopy in the 1800–400 cm-1range, and Partial Least Squares (PLS) regression method was used to build calibration models to predict the absolute combustion temperature of the archaeological woods. Results showed successful PLS forecasting of burn temperature for eachtaxon(significant (P<0.05) cross validation coefficients). The anthracological and chemometric analysis evidenced differences between thetaxacoming from the two stratigraphic units within the Pit, SUs 72 and 74, suggesting that they may come from two different pyres or two different depositional moments.