Species of the genusPlenodomus(Leptosphaeria) are phytopathogens of the Brassicaceae family, which includes oilseed rape. The spores of these fungi spread by airborne transmission, infect plants, and cause crop losses. The secondary metabolism ofP. lingamandP. biglobosuswas studied and compared, with the main focus being on the ability to produce Extracellular Polymeric Substances (EPS). In spite of the 1.5–2-fold faster growth rate ofP. biglobosuson Czapek-Dox and other screening media, the average yield of EPS in this fungus was only 0.29 g/L, compared to that ofP. lingam(0.43 g/L). In turn,P. biglobosusshowed a higher capacity to synthesise IAA, i.e., 14 µg/mL, in contrast to <1.5 µg/mL produced byP. lingam. On the other hand, theP. lingamstrains showed higher β-glucanase activity (350–400 mU/mL), compared to 50–100 mU/mL inP. biglobosus. Invertase levels were similar in both species (250 mU/mL). The positive correlation between invertase activity and EPS yield contrasted with the absence of a correlation of EPS with β-glucanase.Plenodomusneither solubilised phosphate nor used proteins from milk. All strains showed the ability to synthesise siderophores on CAS agar.P. biglobosusexhibited the highest efficiency of amylolytic and cellulolytic activity.