The genusAcrophialophorabelongs to the familyChaetomiaceae. With the addition of new species and transferred species from other genera, the genusAcrophialophorahas expanded. In this study, eight new species related toAcrophialophorawere isolated from soil samples in China. Using muti-locus phylogenetic (ITS, LSU,tub2andRPB2) analysis combined with morphological characteristics, eight new species (Acrophialophora curvata,A. fujianensis,A. guangdongensis,A. longicatenata,A. minuta,A. multiforma,A. rhombica, andA. yunnanensis) are described. Descriptions, illustrations, and notes of the new species are also provided.