Fungal nanotechnology provides techniques useful for molecular and cell biology, medicine, biotechnology, agriculture, veterinary physiology, and reproduction. This technology also has exciting potential applications in pathogen identification and treatment, as well as impressive outcomes in the animal and food systems. Myconanotechnology is a viable option for the synthesis of green nanoparticles because it is simple, affordable, and more environmentally friendly to use fungal resources. Mycosynthesis nanoparticles can be used for various purposes, such as pathogen detection and diagnosis, control, wound healing, drug delivery, cosmetics, food preservation, and textile fabrics, among other applications. They can be applied to a variety of industries, such as agriculture, manufacturing, and medicine. Gaining deeper comprehension of the molecular biology and genetic components underlying the fungal nanobiosynthetic processes is becoming increasingly important. This Special Issue aims to showcase recent advancements in invasive fungal diseases caused by human, animal, plant, and entomopathogenic fungi that are being identified, treated, and treated using antifungal nanotherapy. Utilizing fungus in nanotechnology has several benefits, such as their capacity to create nanoparticles with distinctive characteristics. As an illustration, some fungi can create nanoparticles that are highly stable, biocompatible, and have antibacterial capabilities. Fungal nanoparticles may be used in a variety of industries, including biomedicine, environmental cleanup, and food preservation. Fungal nanotechnology is also a sustainable and environmentally beneficial method. Fungi are an appealing alternative to conventional chemical methods of creating nanoparticles because they are simple to cultivate using affordable substrates and may be cultivated under diverse conditions.