This work provides a complete dataset containing surface electromyography (sEMG) signals acquired from the forearm with a sampling frequency of 1000 Hz. The dataset is named WyoFlex sEMG Hand Gesture and recorded the data of 28 participants between 18 and 37 years old without neuromuscular diseases or cardiovascular problems. The test protocol consisted of sEMG signals acquisition corresponding to ten wrist and grasping movements (extension, flexion, ulnar deviation, radial deviation, hook grip, power grip, spherical grip, precision grip, lateral grip, and pinch grip), considering three repetitions for each gesture. Also, the dataset contains general information such as anthropometric measures of the upper limb, gender, age, laterally of the person, and physical condition. Likewise, the implemented acquisition system consists of a portable armband with four sEMG channels distributed equidistantly for each forearm. The database could be used for the recognition of hand gestures, evaluation of the evolution of patients in rehabilitation processes, control of upper limb orthoses or prostheses, and biomechanical analysis of the forearm.