Simple SummaryCreole goat milk is a valuable product for human consumption. Its physicochemical characteristics could be influenced by altitude and lactation stage. In this work, we evaluated several chemical and physical conditions of Creole goat milk in semiarid rangeland. The main results indicated that protein and density are influenced by altitude, while fat, freezing point, and pH are influenced by the lactation stage. One of the most nutritional values of goat milk is the type and concentration of fatty acids. In this work, we found that the effect of the agroecological region was minimal on their concentration. The lactation stage affected the composition of medium-chain fatty acids and linoelaidic acid. We concluded that nutrition by altitude and goat genetic background could contribute to milk composition.AbstractGoat milk composition is affected by feeding, and in semiarid rangeland, information on Creole goat milk physicochemical composition is lacking. For the fulfillment of this objective, three agroecological regions (AR) considering altitude (lowland 87, highland 779, and mountain 1309 m above sea level) with different botanical compositions were chosen. Every AR analyzed accounted for 30 goat herds, with a total of 90 herds. The results demonstrated that altitude had an influence mainly on density and protein. Milk density increases as altitude increases; conversely, milk protein increases as altitude decreases. On the other hand, in the mountain and lowland ARs, the salts and solids not fat (SNF) percentages were higher compared to that of the highland AR (p< 0.05). The freezing point (FP) was higher at highland altitudes compared to that of mountain and lowland ARs (p< 0.01). In the milk fatty acids (FA) profile, only the C14:1 value was affected by altitude, whereas goat milk at lowland and mountain altitudes had higher values compared to that at highland altitudes (p< 0.05). Additionally, late lactation stage fat, FP, and pH values were higher compared to early lactation values. The opposite effect was observed for salts and SNF. In the FA profile, late lactation values were higher for C10:0 and C8:0 compared to early lactation values. The opposite trend was observed for C18:2n6t. The thrombogenic index was significantly higher at lowland altitudes compared to highland altitudes, and similar to the mountain AR. These goat milk characteristics could be explained as a consequence of animal nutrition, as well as the goat’s meat-type phenotype.