Respiratory diseases caused byMannheimia haemolytica(M.haemolytica) andPasteurella multocida(P. multocida) have been known to result in a considerable loss due to mortality and reduced production. This study aimed at isolation and identification ofM. haemolyticaandP. multocidaassociated with pneumonic pasteurellosis in sheep and goats using bacteriological and molecular techniques. Identification of serotypes ofM. haemolyticaandP. multocidawas done using indirect haemagglutination test. The in vitro antimicrobial sensitivity profiles of theM. haemolyticawere tested using standard disk diffusion method. A total of 52 and 78 nasal swabs were collected from pneumonic cases for bacterial isolation and identification in Borana and Arsi zone, respectively. Four hundred sera samples were collected for identification of serotypes. The results showed that 17 of 52 (32.69%; 95% CI 20.33, 47.11) nasal swabs collected from pneumonic animals in Borana yielded positive results forPasteurella/Mannheimiaspecies, 13 (25.00%; 95% CI 14.03, 38.95) of which wereM. haemolytica. None of the samples yieldedP. multocida. Twenty-three of 78 (29.49%; 95% CI 19.69, 40.89) nasal swabs collected at Arsi from pneumonic animals yielded positive results forM. haemolytica(17) andP. multocida(6). Secondary biochemical characterization revealed that 14 of the 17 isolates conform toM. haemolyticawhereas none of the 6 isolates suspected to beP. mutocidawere confirmed. Eleven (84.62%) isolates from Borana and 4 (28.57%) from Arsi were confirmed to beM. haemolyticausing PCR targeting the Rpt2 genes. Assay forM. haemolyticaserotype A1 revealed all belong to A1. None of the isolates with cultural and morphological features ofP. multocidagave positive results by molecular assay. Serological assay identified three serotypes ofM. haemolyticanamely A1, A2 and A7 almost in all of the samples whereasP. multocidaserotype A was detected in 78.75% of the samples. TheM. haemolyticaisolates tested for susceptibility to antibiotics showed resistance against Bacitracin (83.33%) and Penicillin (50.00%) while they were found susceptible to Gentamycin (100%), Chloramphenicol (100%) and Sulfamethoxazole (100%) and Tetracycline (83.33%). In conclusion, the results of the present study revealed the association ofM. haemolyticawith pneumonic pasteurellosis in sheep and goats and can be of use in vaccine development in Ethiopia. Nevertheless, further investigations and continuous monitoring of antimicrobial resistance and appropriate selection and prudent use of antimicrobials in livestock sector are required.