Electronic tattoos have great potential application in the biomedical field; moreover, the substrate-free electronic tattoo offers better comfortability and conformal contact. However, the substrate-free electronic tattoo is more prone to malfunction, including fall off and fracture. In this paper, a self-healing and self-adhesive substate-free tattoo based on PEDOT: PSS is studied and reported. The dry composite electrode will turn into self-healing material while it transforms into hydrogel, and a cut with a width up to 24 μm could be healed in 1 s. In terms of adhesion performance, the substrate-free electrode can hang a 28.2 g weight by a contact area of 8 mm × 8 mm. Additionally, the substate-free electrode could maintain fully conformal contact with porcine skin in 15 days by its self-adhesiveness. When applied as a substrate-free tattoo, the contact impedance and ECG signal measurement performance before and after self-healing are almost the same. At a frequency of 10 Hz, the contact impedance of the undamaged electrode, healed electrode, and Ag/AgCl gel electrode are 32.2 kΩ, 39.2 kΩ, and 62.9 kΩ, respectively. In addition, the ECG signals measured by the undamaged electrode and healed electrode are comparable to that of Ag/AgCl electrode. The self-healing and self-adhesive substrate-free tattoo electrode reported here has broad application in health monitoring.