BackgroundThe prevalence ofHelicobacter pylori(H. pylori) keeps rising while the eradication rate continues to decline due to the increasing antibiotic resistance. Regional variations of antimicrobial resistance toH. pylorihave been recommended by guidelines in recent years. This study aims to investigate the antibiotic resistance rate ofH. pyloriand its association with infected subjects’ characteristics in Liaoning Province, an area in north China.MethodsGastric tissues from 178H. pyloripositive participants without previous antibiotic use within four weeks were collected forH. pyloriculture. Antibiotic susceptibility to furazolidone (AOZ), tetracycline (TC), levofloxacin (LFX), metronidazole (MET), clarithromycin (CLA), and amoxicillin (AMX) were examined with the agar dilution method. Associations betweenH. pyloriresistance and patient characteristics were further analysed.ResultsNo resistance was observed in AOZ or TC. For LFX, MET, CLA, and AMX, the overall resistance rates were 41.10%, 79.14%, 71.78%, and 22.09% respectively. There were significant differences between resistance to CLA and MALToma (P= 0.021), and between resistance to MET and age (P< 0.001).ConclusionsThe primary resistant rates of LEX, MET, CLA, and AMX were relatively high in Liaoning. Treatment effectiveness improvement could be achieved by prior antimicrobial susceptibility tests before antibiotic prescription.