Insect carboxylesterases (CXEs) can be expressed in multiple tissues and play crucial roles in detoxifying xenobiotic insecticides and degrading olfactory cues. Therefore, they have been considered as an important target for development of eco-friendly insect pest management strategies. Despite extensive investigation in most insect species, limited information on CXEs in sibling moth species is currently available. TheEctropis obliquaProut andEctropis grisescensWarren are two closely related tea geometrid species, which share the same host of tea plant but differ in geographical distribution, sex pheromone composition, and symbiotic bacteria abundance, providing an excellent mode species for studies of functional diversity of orthologous CXEs. In this study, we focused onEoblCXE14due to its previously reported non-chemosensory organs-biased expression. First, theEoblCXE14orthologous geneEgriCXE14was cloned and sequence characteristics analysis showed that they share a conserved motif and phylogenetic relationship. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to compare the expression profiles between twoEctropisspp. The results showed thatEoblCXE14was predominately expressed inE. obliqualarvae, whereasEgriCXE14was abundant inE. grisescensat multiple developmental stages. Interestingly, both orthologousCXEswere highly expressed in larval midgut, but the expression level ofEoblCXE14inE. obliquamidgut was significantly higher than that ofEgriCXE14inE. grisescensmidgut. In addition, the potential effect of symbiotic bacteriaWolbachiaon theCXE14was examined. This study is the first to provide comparative expression profiles of orthologousCXEgenes in two sibling geometrid moth species and the results will help further elucidate CXEs functions and identify a potential target for tea geometrid pest control.