Thirteen knownYrgene-associated markers pertaining to genes (Yr5,Yr10,Yr15,Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes exhibited highly resistant response, with a final rust severity (FRS) ranging from 0 to TR. Seven genotypes expressed a resistant to moderately resistant response with FRS ranging from 5MR–10S. In race-specific phenotying against most prevalent pathotypes ofPucciniastriiformistritici(46S119,110S119 &238S119) by seedling reaction test (SRT) 14 genotypes (29.2%) were found to be immune (IT = 0), 28 genotypes (58.3%) were resistant (IT = 1), and 3 genotypes (6.3%) were moderately resistant (IT = 2).Yr5was detected in sixteen lines with the help of two markersXwmc175andXgwm120linked withYr5.Yr10was detected in ten lines with the markerXpsp3000andYr15was detected in fourteen lines with two linked markers;Xgwm413andXgwm273.Likewise,Yr24/26was detected in 15 lines with two linked markers, namelyXbarc181andXbarc187.Based on the race specific phenotyping data and marker data, fourteen lines were found to carry a single gene, 16 showed the presence of two gene combinations, and seven genotypes were found to have a combination of three genes. Frequencies ofYr5,Yr15andYr26/Yr24was high among test wheat germplasm in comparison toYr10.