Wound healing and tissue repair are recognized as basic human health problems worldwide. Attempts to accelerate the reparative process are focused on developing functional wound dressings. Herein, we present novel Janus textiles with anisotropic wettability from hierarchical microfluidic spinning for wound healing. The hydrophilic hydrogel microfibers from microfluidics are woven into textiles for freeze-drying treatment, followed by the deposition of electrostatic spinning nanofibers composed of hydrophobic polylactic acid (PLA) and silver nanoparticles. The electrospun nanofiber layer can be well coupled with the hydrogel microfiber layer to generate Janus textiles with anisotropic wettability due to the roughness of the hydrogel textile surface and the incomplete evaporation of PLA solution when reaching the surface. For wound treatment with the hydrophobic PLA side contacting the wound surface, the wound exudate can be pumped from the hydrophobic to the hydrophilic side based on the wettability differential derived drainage force. During this process, the hydrophobic side of the Janus textile can prevent excess fluid from infiltrating the wound again, preventing excessive moisture and preserving the breathability of the wound. In addition, the silver nanoparticles contained in the hydrophobic nanofibers could impart the textiles with good antibacterial effect, which further promote the wound healing efficiency. These features indicate that the described Janus fiber textile has great application potential in the field of wound treatment.