PGPR (Plant Growth Promoting Rhizobacteria) are used as biofertilizers and biological control agents against fungi. The objective of this work was to evaluate the antagonistic activities of some bacterial strains isolated from soil against four phytopathogenic fungal strains (Fusarium graminearum,F. culmorum,Phytophthorasp. andVerticillium dahlia). Two strains having an antagonist effect on fungi and displaying the maximum of plant growth promoting (PGP) traits were selected for further study and identified asBacillus subtilisandB. amyloliquefaciensrespectively.In plantaassays demonstrated that the twoBacillusstrains are able to enhance plant growth of two wheat cultivars in absence of nitrogen and protect them againstF. culmorum. Pot experiments performed in a greenhouse showed that wheat plants inoculation with two bacterial strains reduceF. culmorumdisease severity correlated with the accumulation of phenolic compounds and chlorophyll content. These could partly explain the effectiveness of these bacteria in protecting Tunisian durum wheat cultivars againstF. culmorum. ApplicationB. amyloliquefaciens, showed better protection thanB. subtilisalthough the last one enhanced more the plant growth of two wheat cultivars in absence of fungus. Hence, combination of two bacterial strains could be a strategic approach to enhance plant growth and control plant diseases.