Whole genome sequencing data of 874Escherichia coliisolates carryingblaNDM-5from 13 European Union/European Economic Area countries between 2012 and June 2022 showed the predominance of sequence types ST167, ST405, ST410, ST361 and ST648, and an increasing frequency of detection. Nearly a third (30.6%) of these isolates were associated with infections and more than half (58.2%) were predicted to be multidrug-resistant. Further spread ofE. colicarryingblaNDM-5would leave limited treatment options for seriousE. coliinfections.