AbstractThis scientific report provides an update of theXylellaspp. host plant database, aiming to provide information and scientific support to risk assessors, risk managers and researchers dealing withXylellaspp. Upon a mandate of the European Commission, EFSA created and regularly updates a database of host plant species ofXylellaspp. The current mandate covers the period 2021–2026. This report is related to the eighth version of the database published in Zenodo in the EFSA Knowledge Junction community, covering literature published from 1 July 2022 up to 31 December 2022, and recent Europhyt outbreak notifications. Informative data have been extracted from 21 selected publications. Twelve new host plants were identified and added to the database. Nine plant species were reported from Portugal and naturally infected by subsp.multiplexor unknown (i.e. not reported). Three plant species were successfully artificially infected by subsp.fastidiosa. No additional data were retrieved forX. taiwanensis, and no additional STs were identified worldwide. New information on the tolerant/resistant response of plant species toX. fastidiosainfection were added to the database. The overall number ofXylellaspp. host plants determined with at least two different detection methods or positive with one method (between sequencing and pure culture isolation) reaches now 433 plant species, 197 genera and 68 families. Such numbers rise to 690 plant species, 306 genera and 88 families if considered regardless of the detection methods applied.