In this study, a total of 177NACmembers were identified inAvena sativa, located on 21 chromosomes. Phylogenetic analysis showed that AsNAC proteins could be divided into seven subfamilies (I–VII), and that proteins in the same subfamily have similar protein motifs. Gene structure analysis found thatNACintrons ranged from 1 to 17. Cis-element analysis of the promoter indicated that the gene family may have stress-related elements and growth regulation elements. Through qRT-PCR experiments, we speculated thatAsNACsgenes can respond to abiotic stresses such as cold, freezing, salt, and saline alkali. This study provides a theoretical basis for further exploring the function of theNACgene family inA. sativa.