The guided waves in the uniform waveguide of rectangular cross-section exhibit complicated propagation and scattering characteristics due to the diversity of vibration modes. This paper focuses on the mode conversion of the lowest Lamé mode at a part-through or through-thickness crack. Firstly, the Floquet periodicity boundary condition is applied to derive the dispersion curves in the rectangular beam, which relates the axial wavenumber to the frequency. On this basis, the frequency domain analysis is conducted to investigate the interaction between the fundamental longitudinal mode in the vicinity of the first Lamé frequency and a part-through or through-thickness vertical or inclined crack. Finally, the nearly perfect transmission frequency is evaluated by extracting displacement and stress harmonic fields throughout the cross-section. It is shown that this frequency originates from the first Lamé frequency, increases with the crack depth, and decreases with the crack width. Between them, the crack depth plays a major role in the frequency variation. In addition, the nearly perfect transmission frequency is negligibly affected by the beam thickness, and such a phenomenon is not observed for inclined cracks. The nearly perfect transmission may have potential applications in the quantitative evaluation of crack size.