Light-emitting diodes are increasingly used as artificial light sources inHaematococcus pluvialiscultivation due to the fact of their energy advantages. The immobilized cultivation ofH. pluvialisin pilot-scale angled twin-layer porous substrate photobioreactors (TL-PSBRs) was initially performed with a 14/10 h light/dark cycle and showed relatively low biomass growth and astaxanthin accumulation. In this study, the illumination time with red and blue LEDs at a light intensity of 120 µmol photons m−2s−1was increased to 16–24 h per day. With a light/dark cycle of 22/2 h, the biomass productivity of the algae was 7.5 g m−2day−1, 2.4 times higher than in the 14/10 h cycle. The percentage of astaxanthin in the dry biomass was 2%, and the total amount of astaxanthin was 1.7 g m−2. Along with the increase in light duration, adding 10 or 20 mM NaHCO3to the BG11-H culture medium over ten days of cultivation in angled TL-PSBRs did not increase the total amount of astaxanthin compared with only CO2addition at a flow rate of 3.6 mg min−1to the culture medium. Adding NaHCO3with a 30–80 mM concentration inhibited algal growth and astaxanthin accumulation. However, adding 10–40 mM NaHCO3caused algal cells to accumulate astaxanthin at a high percentage in dry weight after the first four days in TL-PSBRs.