Enterococcus faecalisis a well-established resident of the human gastrointestinal tract and is also a major cause of human infections. Unfortunately, therapeutic options forE. faecalisinfections remain limited, particularly with the emergence of vancomycin-resistant strains in hospital settings. Consequently, there has been a renewed interest in phage therapy as an alternative to antibiotics. In this study, we have isolated a bacteriophage, vB_EfaS-SFQ1, from hospital sewage, which effectively infectsE. faecalisstrain EFS01. Phage SFQ1 is a siphovirus and exhibits a relatively broad host range. Furthermore, it has a short latent period of approximately 10 min and a large burst size of about 110 PFU/cell at a multiplicity of infection (MOI) of 0.01, and it could effectively disrupt the biofilms formed byE. faecalis. Thus, this study provides a detailed characterization ofE. faecalisphage SFQ1, which has great potential for treatingE. faecalisinfections.