Fungi-mediated self-healing concrete is a novel approach that promotes the precipitation of calcium carbonate (CaCO3) on fungal hyphae to heal the cracks in concrete. In this study, we set out to explore the potential of fungal species isolated from a limestone cave by investigating their ability to precipitate CaCO3and to survive and grow in conditions relevant to concrete. Isolated strains belonging to the generaBotryotrichum sp.,Trichoderma sp.andMortierella sp.proved to be promising candidates for fungi-mediated self-healing concrete attributed to their growth properties and CaCO3precipitation capabilities in the presence of cement.