The widespread use of antibiotics in recent years has led to increasing antibiotic contamination of shallow groundwater. As the most widely used tetracycline antibiotic, oxytetracycline has received a lot of attention from researchers due to its stable molecular structure and difficulty in degradation. Aiming at remediation of oxytetracycline pollution in shallow groundwater, nano-calcium peroxide (nCaO2) and ozone (O3) are used to enhance the degradation of oxytetracycline in groundwater circulation well (GCW). A three-dimensional sand box test device for circulation wells is designed to explore the repair efficiency of circulation wells strengthened by different oxidants. The results show that after nCaO2and O3enhancing circulation wells operate for 10 h, the average removal rate of OTC reaches 83%, and the highest removal rate is 88.13%, which is 79.23% and 13.96% respectively higher than that of nCaO2and O3enhanced circulation wells alone, and there is no rebound phenomenon after aeration stops. The in-situ treatment of enhanced GCW by nCaO2and O3has potential applications for the removal of OTC in groundwater environments.