Simple SummaryGeographical variability of acoustic signals is studied more often in songbird species, which does not include the black grouse. The black grouse males use different calls during the mating performance, including vocal rookooing. No studies have yet dealt with a more detailed analysis of these signals, except one. Although the studied hissing calls of this species are classified as non-vocal acoustic signals, individuality was observed in these calls. To discern whether there is also geographical variability across the range or distribution area of the black grouse, we analyzed and compared hissing calls from four countries. Individuals in the Czech Republic, Finland, Scotland, and Russia were recorded during mating seasons. The results of the analysis revealed differences between the four subpopulations, although not at the level of dialect distinction. The study of acoustic variability, supported by genetic research, will help to better understand the links or, conversely, the differentiation processes between subpopulations.AbstractThe black grouse is a species whose population development requires constant monitoring due to a rapidly declining trend, especially in Central Europe. Variability in the voices of geographically separated populations can aid in counting within individual populations. This has been investigated with the black grouse. However, the variability of the acoustic behavior of black grouse between populations was investigated for the first time. In total, 82 male black grouse were recorded during the lekking season in four countries: the Czech Republic, Scotland, Finland, and Russia. We analyzed recordings of hissing calls, i.e., the non-vocal signal. DFA analysis correctly classified almost 70% of the recordings. The results indicate a certain degree of difference between the grouse populations from the four countries examined. The mean frequency of hissing calls for populations was 1410.71 ± 170.25 Hz, 1473.89 ± 167.59 Hz, 1544.38 ± 167.60 Hz, and 1826.34 ± 319.23 Hz in the Czech Republic, Finland, Russia, and Scotland, respectively. Populations from Scotland and Russia have greater intra-variability compared to grouse from the Czech Republic and Finland, indicating that population density is not the principal factor in the geographical variability of black grouse hissing calls. Range-level differences enhance knowledge and facilitate the assessment of species evolution.