Chicory leaves (Cichorium intybus) are widely consumed due to their health benefits. They are mainly consumed raw or without adequate washing, which has led to an increase in food-borne illness. This study investigated the taxonomic composition and diversity of chicory leaves collected at different sampling times and sites. The potential pathogenic genera (Sphingomonas,Pseudomonas,Pantoea,Staphylococcus,Escherichia, andBacillus) were identified on the chicory leaves. We also evaluated the effects of various storage conditions (enterohemorrhagicE. colicontamination, washing treatment, and temperature) on the chicory leaves’ microbiota. These results provide an understanding of the microbiota in chicory and could be used to prevent food-borne illnesses.