Non-physical barriers for migrating fish, such as effluent plumes discharged by sewage treatment plants (WWTPs), are hardly considered, and field studies on this topic are very scarce. The encounter with these plumes however may evoke behavioural responses in fish and could delay or (partially) block the migration. In this study, the behavioural responses of 40 acoustically-tagged silver eel (Anguilla anguilla)were monitored in situ, when confronting a WWTP effluent plume during their downstream migration in the canal Eems, the Netherlands. Their behavioural responses and the potential blocking effect of the plume were assessed using a 2D and 3D telemetry design displayed in the waterway, and matched to a modelled and calibrated WWTP effluent plume. When confronted with the WWTP effluent plume during their downstream migration, 22 of the silver eels (59%) showed an avoidance response, varying from lateral diverting to multiple turning in the vicinity of the effluent plume. Nineteen out of these 22 (86%) eventually passed the study site. No silver eel showed attraction to the plume. Delays in migration were from several hours up to several days. Due to the strong variation in discharged volumes and flow velocity of the receiving canal, the WWTP plume did not always flow over the full width of the canal. As a result, numerous migratory windows, where silver eels could pass the WWTP while avoiding direct contact with the plume, remained available in time. When discharge points cannot be avoided, reduced or restricted to areas that are not preferred as fish migration routes, discharge points should be designed such, that the chance is limited that a waterway is (temporarily) impacted over its full width.