SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except forOsSPX1research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of otherSPXgenes in cold stress are unknown. Therefore, in this study, we identified sixOsSPXsfrom the whole genome of DXWR. The phylogeny ofOsSPXshas a strong correlation with its motif. Transcriptome data analysis showed thatOsSPXswere highly sensitive to cold stress, and real-time PCR verified that the levels ofOsSPX1,OsSPX2,OsSPX4, andOsSPX6in cold-tolerant materials (DXWR) during cold treatment were higher than that of cold-sensitive rice (GZX49). The promoter region of DXWROsSPXscontains a large number of cis-acting elements related to abiotic stress tolerance and plant hormone response. At the same time, these genes have expression patterns that are highly similar to cold-tolerance genes. This study provides useful information aboutOsSPXs, which is helpful for the gene-function research of DXWR and genetic improvements during breeding.