AbstractCanine visceral leishmaniasis is a disease caused by the protozoonLeishmania infantum, an important agent of zoonotic concern. In this study we determined the seroprevalence, risk factors and spatial distribution of infection byL. infantumamong dogs in the Pajeú microregion of the Sertão region of Pernambuco, Brazil. Canine serum samples (n = 247) were tested using the Dual Path Platform (DPP®) rapid screening test and ELISA/S7® confirmatory test; and risk factors were assessed through univariate analysis and logistical regression. The spatial distribution of reactive dogs was analyzed by drawing a map using QGIS. Seroprevalence of 13.7% (34/247) was detected, with cases predominating in the municipality of Tabira (26.4%; 9/34). Age above 10 years was considered to be a risk factor associated with the presence of anti-L. infantumantibodies. The high overall prevalence and spatial distribution of positive cases showed wide dispersion of reagents dogs in the study area. Therefore, preventive measures are needed in order to reduce the risk of infection for animals and humans.