Highlights•Phages M3, C2, M9, and M83 belong to a new species of the genus ofMaculvirusand phages ALK and CHI belong to new genus of theQueuovirinaesubfamily.•CHI, ALK, M3, C2, M9, and M83 could lyse 50.0% (5/10) of Mexican AHPND-causingVibrio parahaemolyticusstrains.•No transfer RNA (tRNA), virulence, or antibiotic resistance genes were found in phage genomes.•CHI, ALK, M3, C2, M9, and M83 significantly reduced the growth of Mexican AHPND-causingVibrio parahaemolyticusstrains after incubating with MOI of 0.01–1.