This study presents a new method for measuring the propagation constant of transmission lines using a single line standard and without prior calibration of a two-port vector network analyzer (VNA). The method provides accurate results by emulating multiple line standards of the multiline calibration method. Each line standard was realized by sweeping an unknown network along a transmission line. The network need not be symmetric or reciprocal, but must exhibit both transmission and reflection. We performed measurements using a slab coaxial airline and repeated the measurements on three different VNAs. The measured propagation constant of the slab coaxial airline from all VNAs was nearly identical. By avoiding disconnecting or moving the cables, the proposed method eliminates errors related to the repeatability of connectors, resulting in improved broadband traceability to SI units.