ABSTRACTDesulfomicrobiumsp. strain ZS1 is an obligate anaerobic, sulfate-reducing member of theDesulfobacterotafrom Zodletone Spring, an anoxic sulfide-rich spring in southwestern Oklahoma. Its complete genome was sequenced using a combination of Illumina and Oxford Nanopore platforms and encodes 3,364 proteins and 81 RNAs on a single chromosome.