Carotenoid cleavage oxygenase (CCO) is an enzyme that can catalyze carotenoids to volatile aromatic substances and participate in the biosynthesis of two important phytohormones, i.e., abscisic acid (ABA) and strigolactone (SL). However, the genome-wide identification and analysis of theCCOgene family in the rare and endangered woody plantLiriodendron chinensehas not been reported. Here, we performed a genome-wide analysis of theCCOgene family in theL. chinensegenome and examined its expression pattern during different developmental processes and in response to various abiotic stresses. A total of 10LcCCOgenes were identified and divided into 6 subfamilies according to the phylogenetic analysis. Subcellular localization prediction showed that most of the LcCCO proteins were located in the cytoplasm. Gene replication analysis showed that segmental and tandem duplication contributed to the expansion of this gene family in theL. chinensegenome.Cis-element prediction showed thatcis-elements related to plant hormones, stress and light response were widely distributed in the promoter regions ofLcCCOgenes. Gene expression profile analysis showed thatLcNCED3bwas extensively involved in somatic embryogenesis, especially the somatic embryo maturation, as well as in response to heat and cold stress in leaves. Furthermore, qRT-PCR analysis showed thatLcNCED3bobviously responded to drought stress in roots and leaves. This study provides a comprehensive overview of theLcCCOgene family and a potential gene target for the optimization of the somatic embryogenesis system and resistance breeding in the valuable forest treeL. chinense.