Highlights•MAYV RNA synthesis starts at 2hpi, Morphogenesis at 3hpi and Liberation at 4hpi.•Ribosome reorganization precedes early morphogenesis/liberation of particles.•Clusters of nucleocapsid-like structures are related with early morphogenesis.•CPV-II formation was correlated with exponential increase of virus particles.•Timeline of events of MAYV replication was established.