Pseudomonas aeruginosais a difficult-to-treat pathogen that is frequently involved with chronic wound infections. Here, we conducted a literature search of world-wide studies published between 2005 and 2022 that described the microbiological profiles of chronic wound infections. For each continent, a hierarchy of pathogens was created to define the organisms that were most frequently isolated in each region. Except for South America,P. aeruginosawas the second most common organism in each major continent, withStaphylococcus aureusbeing the most abundant pathogen overall. When individual countries were evaluated,P. aeruginosawas the most frequently isolated organism in several Southeast Asia nations including India and Malaysia.P. aeruginosawas less commonly isolated from diabetic foot infections in North America, Europe, and Africa in comparison to other types of chronic wound infections. Additionally, the Levine wound swab technique may be a quick and painless way to isolateP. aeruginosafrom wound infections, but the isolation ofP. aeruginosadoes not seem to be an informative predictor of the patient’s clinical course. A multivariate risk assessment that accounts for the regional frequency ofP. aeruginosaisolation may be an appropriate way to guide empiric management of chronic wound infections.