AbstractDespite relatively high maturation rate ofin vitromatured oocytes in the dromedary camel, however, blastocyst production is very low afterin vitrofertilization (IVF). Herein, the influences of oocyte collection method (follicular aspirationvsslicing;Experiment I), the addition of Insulin-like growth factor I (IGF-I) to the maturation medium (Experiment II) onin vitromaturation (IVM) of oocyte were investigated. Although the nuclear maturation did not differ regardless of collecting method, follicular aspiration led to lower degeneration rates than those in controls (P < 0.05). The percentages of oocytes at MII were greater in the presence of IGF-1 than in its absence (71.9% vs 48.4%, respectively, P<0.05). Additionally, the percentages of degenerated oocytes were higher in the control group compared to oocytes cultured in the presence of IGF-I (23.6%vs10.4%, respectively, P<0.05). IGF-I treatment improved the quality of MII matured oocytes as evidenced by the decrease of cathepsin B (CTSB) activity, a marker of poor quality oocytes, when compared to control ones (P < 0.05). In conclusion, follicular aspiration decreased the degeneration rate; however, it had no effect on completion of maturation. IGF-I enhanced the IVM of oocyte and decreased degeneration rate.