IntrodutionFungal endophytes are widespread and dwell inside plant cells for at least part of their life without producing any symptoms of infection. Distinct host plants may have different fungal endophyte rates and community compositions. Despite this, the endophytic fungi connected with the host plant and their hostile behaviors, remain unknown.MethodsThe objective of the current research was to isolate and identify endophytic fungal species from the root ofAndrographis paniculata. The effects of fungal isolate APR5 on the mycelial growth of phytopathogens and the production of plant-promoting traits were assessed.Results and discussionEndophytic fungal isolate APR5 showed higher inhibitory efficacy in dual and double plate assay against the tested phytopathogenic fungi. The scanning electron microscope analysis demonstrated that the phytopathogenic fungal hyphae were coiled by endophytes which makes them shrink and disintegrate. Further, an ethyl acetate crude extract effectively suppressed the mycelium growth ofRhizoctonia solaniby 75 ± 0.1% in an agar well diffusion assay. The fungal isolate APR5 was identified asLasiodiplodia pseudotheobromaeusing the nuclear ribosomal DNA internal transcribed spacer (ITS) region and qualitatively evaluated for their capacity to produce plant growth-promoting hormones. Gas chromatography-mass spectrometry was implemented to acquire a preliminary understanding of the secondary metabolic profile of ethyl acetate crude extract. 1-octadecene, erythritol, niacin, oleic acid, phenol, pantolactone, phenyl ethyl alcohol,p-cresol, and tbutyl hydroquinone are the metabolites analyzed in a crude extract of APR5 isolate and are reported to have antimicrobial properties.