Ethylene has an important role in regulating plant growth and development as well as responding to adversity stresses. The 1-aminocyclopropane-1-carboxylate synthase (ACS) is the rate-limiting enzyme for ethylene biosynthesis. However, the role of theACSgene family in wheat has not been examined. In this study, we identified 12ACSmembers in wheat. According to their position on the chromosome, we named themTaACS1-TaACS12, which were divided into four subfamilies, and members of the same subfamilies had similar gene structures and protein-conserved motifs. Evolutionary analysis showed that fragment replication was the main reason for the expansion of theTaACSgene family. The spatiotemporal expression specificity showed that most of the members had the highest expression in roots, and allACSgenes contained W box elements that were related to root development, which suggested that theACSgene family might play an important role in root development. The results of the gene expression profile analysis under stress showed thatACSmembers could respond to a variety of stresses. Protein interaction prediction showed that there were four types of proteins that could interact with TaACS. We also obtained the targeting relationship betweenTaACSfamily members and miRNA. These results provided valuable information for determining the function of the wheatACSgene, especially under stress.