ObjectiveThe purpose of this study was evaluating the biaxial strength of bi-layered PEEK restorations before and after aging using different veneering materials in different thickness ratios.Material and MethodsNinety specimens of thickness 1.5 mm were divided into three groups according to their veneering material. Group (CAD LD): BioHPP discs veneered with CAD milled lithium disilicate (n=30), group (CAD C): BioHPP discs veneered with CAD milled composite (n=30), and group (LC): BioHPP discs veneered with conventionally layered composite (n=30). Each group was subdivided into 3 subgroups (n=10) according to the different thickness ratios between the core and the veneering material (TC:TV). Subgroup 1: TC:TV=1:0.5, subgroup 2: TC:TV=0.7:0.8, and subgroup 3: TC:TV=0.5:1. Half of the specimens of each subgroup were subjected to thermocycling, and the bi-axial flexural strength of all specimens was tested before and after aging. Three-way ANOVA followed by Bonferroni’s post hoc test were used for data analysis. The significance level was set atP≤ 0.05.ResultsMaterial, thickness ratio, and aging all had a significant effect on biaxial flexural strength. (LC) group had the highest biaxial flexural strength. TC:TV=0.5:1 showed the lowest biaxial flexural strength. All groups showed significant decrease in biaxial flexural strength after aging.ConclusionsVeneering material for PEEK together with the thickness ratio between the core and veneering material greatly affect the flexural strength of bi-layered restorations. Thermocycling negatively impacts the flexural strength of PEEK bi-layered restorations.Clinical significanceAccording to the results of that study, PEEK cores are best veneered with conventionally layered composite with core to veneering thickness ratio being 1:0.5.