This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genesoptrAandcfr(D) inStreptococcus parasuis. In total, 36Streptococcusisolates (30Streptococcus suisisolates, 6Streptococcus parasuisisolates) were collected from pig farms in China in 2020–2021, using PCR to determine the presence ofoptrAandcfr. Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of theoptrAandcfr(D) genes. Conjugation and inverse PCR were employed to verify the transferability ofoptrAandcfr(D). TheoptrAandcfr(D) genes were identified in twoS. parasuisstrains named SS17 and SS20, respectively. TheoptrAof the two isolates was located on chromosomes invariably associated with thearaCgene and Tn554, which carry the resistance geneserm(A) andant(9). The two plasmids that carrycfr(D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. Thecfr(D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background ofoptrAandcfr(D) and indicate that Tn554and IS1202 may play an important role in the transmission ofoptrAandcfr(D), respectively.