﻿AbstractColletotrichumspecies are well-known plant pathogens, saprobes, endophytes, human pathogens and entomopathogens. However, little is known aboutColletotrichumas endophytes of plants and cultivars includingCitrusgrandiscv. “Tomentosa”. In the present study, 12 endophyticColletotrichumisolates were obtained from this host in Huazhou, Guangdong Province (China) in 2019. Based on morphology and combined multigene phylogeny [nuclear ribosomal internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (gapdh), chitin synthase 1 (chs-1), histone H3 (his3) actin (act), beta-tubulin (β-tubulin) and glutamine synthetase (gs)], sixColletotrichumspecies were identified, including two new species, namelyColletotrichumguangdongenseandC.tomentosae.Colletotrichumasianum,C.plurivorum,C.siamenseandC.tainanenseare identified as being the first reports onC.grandiscv. “Tomentosa” worldwide. This study is the first comprehensive study on endophyticColletotrichumspecies onC.grandiscv. “Tomentosa” in China.