﻿AbstractRecent surveys in the Río Negro-Sopladora National Park revealed a striking new species ofHyloscirtus. The new species is easily diagnosed from all other congeners by its large body size (64.9 mmSVLin adult female); broad dermal fringes in fingers and toes; prepollex not projected into a prepollical spine and hidden under thenar tubercle; dorsum greyish-green, with paler-hued reticulum, yellow spots and black speckles; throat, venter, flanks and hidden surfaces of limbs golden-yellow with large black blotches and spots; fingers, toes and webbing yellow with black bars and spots; iris pale pink with black periphery. It is currently known only from its type locality, in the high montane forest on the southern slopes of the Cordillera Oriental of the Andes, southeastern Ecuador. The new species might be related to theH.larinopygionspecies group based on its morphology.