Diaphorina citri, a vector of citrus huanglongbing (HLB) disease, frequently leads to HLB outbreaks and reduces Rutaceae crop production. Recent studies have investigated the effects of RNA interference (RNAi) targeting theVitellogenin(Vg4) andVitellogenin receptor(VgR) genes, which are involved in egg formation in this pest, providing a theoretical foundation for developing new strategies to manageD. citripopulations. This study presents RNAi methods forVg4andVgRgene expression interference and reveals that dsVgRis more effective than dsVg4againstD. citri. We demonstrated that dsVg4and dsVgRpersisted for 3–6 days inMurraya odoriferashoots when delivered via the in-plant system (IPS) and effectively interfered withVg4andVgRgene expression. FollowingVg4andVgRgene expression interference, egg length and width in the interference group were significantly smaller than those in the negative control group during the 10–30-day development stages. Additionally, the proportion of mature ovarian eggs in the interference group was significantly lower than that in the negative control group at the 10, 15, 20, 25, and 30-day developmental stages. DsVgRnotably suppresses oviposition inD. citri, with fecundity decreasing by 60–70%. These results provide a theoretical basis for controllingD. citriusing RNAi to mitigate the spread of HLB disease.