ABSTRACTIncidence of vibriosis is rising globally, with evidence that changing climatic conditions are influencing environmental factors that enhance growth of pathogenicVibriospp. in aquatic ecosystems. To determine the impact of environmental factors on occurrence of pathogenicVibriospp., samples were collected in the Chesapeake Bay, Maryland, during 2009 to 2012 and 2019 to 2022. Genetic markers forVibrio vulnificus(vvhA) andVibrio parahaemolyticus(tlh,tdh, andtrh) were enumerated by direct plating and DNA colony hybridization. Results confirmed seasonality and environmental parameters as predictors. Water temperature showed a linear correlation withvvhAandtlh, and two critical thresholds were observed, an initial increase in detectable numbers (>15°C) and a second increase when maximum counts were recorded (>25°C). Temperature and pathogenicV. parahaemolyticus(tdhandtrh) were not strongly correlated; however, the evidence showed that these organisms persist in oyster and sediment at colder temperatures. Salinity (10 to 15 ppt), total chlorophylla(5 to 25 μg/L), dissolved oxygen (5 to 10 mg/L), and pH (8) were associated with increased abundance ofvvhAandtlh. Importantly, a long-term increase inVibriospp. numbers was observed in water samples between the two collection periods, specifically at Tangier Sound (lower bay), with the evidence suggesting an extended seasonality for these bacteria in the area. Notably,tlhshowed a mean positive increase that was ca. 3-fold overall, with the most significant increase observed during the fall. In conclusion, vibriosis continues to be a risk in the Chesapeake Bay region. A predictive intelligence system to assist decision makers, with respect to climate and human health, is warranted.IMPORTANCEThe genusVibrioincludes pathogenic species that are naturally occurring in marine and estuarine environments globally. Routine monitoring forVibriospecies and environmental parameters influencing their incidence is critical to provide a warning system for the public when the risk of infection is high. In this study, occurrence ofVibrio parahaemolyticusandVibrio vulnificus, both potential human pathogens, in Chesapeake Bay water, oysters, and sediment samples collected over a 13-year period was analyzed. The results provide a confirmation of environmental predictors for these bacteria, notably temperature, salinity, and total chlorophylla, and their seasonality of occurrence. New findings refine environmental parameter thresholds of culturableVibriospecies and document a long-term increase inVibriopopulations in the Chesapeake Bay. This study provides a valuable foundation for development of predicative risk intelligence models forVibrioincidence during climate change.