BackgroundTrypanosoma cruzicauses Chagas disease (CD), a potentially fatal disease characterized by cardiac disorders and digestive, neurological or mixed alterations.T.cruziis transmitted to humans by the bite of triatomine vectors; both the parasite and disease are endemic in Latin America and the United States. In the last decades, population migration has changed the classic epidemiology ofT.cruzi, contributing to its global spread to traditionally non-endemic countries. Screening is recommended for Latin American populations residing in non-endemic countries.MethodsThe present study analyzes the epidemiological characteristics of 2,820 Latin American individuals who attended the International Health Service (IHS) of the Hospital Clinic de Barcelona between 2002 and 2019. The initial assessment of organ damage among positive cases ofT.cruziinfection was analyzed, including the results of electrocardiogram (ECG), echocardiogram, barium enema and esophagogram.ResultsAmong all the screened individuals attending the clinic, 2,441 (86.6%) were born in Bolivia and 1,993 (70.7%) were female. Of individuals, 1,517 (81.5%) reported previous exposure to the vector, which is a strong risk factor associated withT.cruziinfection; 1,382 individuals were positive forT.cruziinfection. The first evaluation of individuals with confirmedT.cruziinfection, showed 148 (17.1%) individuals with Chagasic cardiomyopathy, the main diagnostic method being an ECG and the right bundle branch block (RBBB) for the most frequent disorder; 16 (10.8%) individuals had a normal ECG and were diagnosed of Chagasic cardiomyopathy by echocardiogram.ConclusionsWe still observe many Latin American individuals who were at risk ofT.cruziinfection in highly endemic areas in their countries of origin, and who have not been previously tested forT.cruziinfection. In fact, even in Spain, a country with one of the highest proportion of diagnosis of Latin American populations,T.cruziinfection remains underdiagnosed. The screening of Latin American populations presenting with a similar profile as reported here should be promoted. ECG is considered necessary to assess Chagasic cardiomyopathy in positive individuals, but echocardiograms should also be considered as a diagnostic approach given that it can detect cardiac abnormalities when the ECG is normal.