Physical activity is essential for women with physical disabilities. This review aims to identify the barriers they face in practicing sport. A systematic review was conducted using the PubMed/Medline, Scopus, and Web of Science databases in January 2023, with an update in March 2023. The eligibility criteria used for inclusion were as follows. (i) Women with physical disabilities; (ii) women who engage in or want to engage in physical activities and/or sport, both adapted and non-adapted; (iii) identification of women’s barriers to such practice; (iv) research articles; and (v) papers written in English and published in peer-reviewed journals. The exclusion were as follows. (i) Women with illness, injury or transient physical activity difficulties; (ii) mention of rehabilitative physical activity; and (iii) results showing no differentiation in barrier types by gender. This review identified different barriers, grouped into eight types according to the differentiating factor, thus showing that disable people’s participation in physical activity is directly related to some specific barriers which seem to differ according to their gender. Therefore, the success of participation in physical activities depends not only on the user’s concern, but also on an inclusive social environment.