Background:Cervical cancer (CC) remains one of the most common and deadly malignancies in women worldwide.FBXO5, a protein-coding gene, is highly expressed in a variety of primary tumors and promotes tumor progression, however, its role and prognostic value in CC remain largely unknown.Methods:A key differential gene,FBXO5, was screened according to WGCNA based on immunohistochemical assays of clinical samples, multiple analyses of the Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases, including survival analysis, tumor mutational burden, GO, KEGG, tumor immune infiltration, and chemotherapeutic drug sensitivity, to explore the expression and prognostic value ofFBXO5in CC. The migration and invasiveness of cervical cancer cells followingFBXO5knockdown and overexpression were examined using wound healing and transwell assays, and the viability of cancer cells was assessed using CCK8 and EdU assays.Results:FBXO5was discovered to be substantially expressed in CC tissues using data from our CC cohort and the TCGA database, and a survival analysis indicatedFBXO5as a predictive factor for poor overall survival in CC patients.In vitro, CC cells were more inclined to proliferate, migrate, and invade whenFBXO5was upregulated as opposed to when it was knocked down.