Cucumber (Cucumis sativusL.) is an important vegetable worldwide, but its yield is affected by a wide range of pathogens and pests. As the major subunit of the exocyst complex, the roles of Exo70 members have been shown inArabidopsisand rice, but their function are unknown in cucumber. Here, we identified 18 CsExo70 members in cucumber, which were divided into three groups (Exo70.1–Exo70.3) and nine subgroups (Exo70A–Exo70I) based on the phylogenetic tree. Subsequently, systematical analyses were performed, including collinearity, gene structure,cis-acting elements, conserved motifs, expression patterns, and subcellular localization. Our results showed thatCsExo70genes were generally expressed in all tissues, andCsExo70C1andCsExo70C2were highly expressed in the stamen. Moreover, the expression levels of mostCsExo70genes were induced byPseudomonas syringaepv.lachrymans(Psl) andFusarium oxysporumf. sp.cucumerinumOwen (Foc), especiallyCsExo70E2andCsExo70H3. In addition, these CsExo70s displayed similar location patterns with discrete and punctate signals in the cytoplasm. Together, our results indicate that CsExo70 members may be involved in plant development and resistance, and provide a reference for future in-depth studies ofExo70genes in cucumber.