Plants must be able to sense and respond to mechanical stresses encountered throughout their lifespan. The MscS-Like (MSL) family of mechanosensitive ion channels is one mechanism to perceive mechanical stresses. In maize, brace roots emerge from stem nodes above the soil and some remain aerial while some grow into the soil. We tested the hypothesis that MSL gene expression is higher in subterranean brace roots compared to those that remain aerial. However, there was no difference in MSL expression between the two environments. This work sets the foundation for a deeper understanding of MSL gene expression and function in maize.