This study investigated the exposure of the cotton bollworm,Helicoverpa zea(Boddie) (Lepidoptera: Noctuidae) to a novel pathogenic fungal agent historically associated with human medicinal value, a commercial strain ofCordyceps militaris((L.) Fr.) Vuill. (Hypocreales). A series of comparative studies were conducted to evaluate the efficacy of two different exposure methods using four concentrations (n× 109,n× 108,n× 107,n× 106) ofC. militaris, wheren× 109provided a concentration of approximately 420 ± 37 spores per mm2with 398 ± 28 viable spores. Survival of cotton bollworms of all stages was not affected byC. militarisat any concentration 1 d post-exposure. The greatest reduction in survival and highest sporulation rates were observed primarily on or after 7 d post-exposure for early instars (first and second). Significant declines in the survival of early instars were observed for all concentrations at 7 d, and 95% mortality by 10 d, with the exception of the fifth instars that experienced a less severe reduction in survival (35%) when exposed to any concentrations used in the study. Survival of late instars (third to fifth) ranged from 44% to 68% on day 10, while adult survival was near 99% across the duration of the experiment. The relatively narrow range observed for both the lethal concentration and sporulation of second, third, and fifth instar cotton bollworms exposed to theC. militarisstrain may demonstrate potential field application for control of larval populations of cotton bollworms.