Higher education is one of the types of education most influenced by digital technologies. This situation, in educational contexts of quality and equity, presents different advantages but, at the same time, also many challenges. One of them is the use of ICT to support students with disabilities. In this line, the main objective of this study is to evaluate an instrument to measure the level of training and knowledge of university teachers in Spain concerning the application of ICT as support for students with disabilities. For the validation of content, the technique of “expert judgement” was used, applying a process of expert selection called “Expert Competence Coefficient” or “K Coefficient”. The instrument's reliability index was obtained through two statistics, Cronbach's alpha and McDonald's Omega. From the results obtained, it is confirmed that the questionnaire under study is an instrument with evidence of validity and reliability that allows us to diagnose, among university teaching staff, sub-dimensions of special relevance to find out the level of training and knowledge they have about ICT and students with disabilities.