Exposure to extreme temperatures can hinder the development of insects and even reduce their survival rate. However, the invasive speciesBemisia tabaciexhibits an impressive response to different temperatures. This study aims to identify important transcriptional changes ofB. tabacioccupying different temperature habitats by performing RNA sequencing on populations originating from three regions of China. The results showed that the gene expression ofB. tabacipopulations inhabiting regions with different temperatures was altered and identified 23 potential candidate genes that respond to temperature stress. Furthermore, three potential regulatory factors’ (the glucuronidation pathway, alternative splicing, and changes in the chromatin structure) response to different environmental temperatures were identified. Among these, the glucuronidation pathway is a notable regulatory pathway. A total of 12 UDP-glucuronosyltransferase genes were found in the transcriptome database ofB. tabaciobtained in this study. The results of DEGs analysis suggest that UDP-glucuronosyltransferases with a signal peptide may helpB. tabaciresist temperature stress by sensing external signals, such asBtUGT2C1andBtUGT2B13, which are particularly important in responding to temperature changes. These results will provide a valuable baseline for further research on the thermoregulatory mechanisms ofB. tabacithat contributes to its ability to effectively colonize regions with considerable temperature differences.