Because of its distinct geological history, frigid temperature, and rich biodiversity, the Tibetan Plateau gives an excellent opportunity to assess the effect of climate change on determining species richness. The distribution patterns of fern species richness and their underlying processes have long been a matter of debate in ecology research, with various hypotheses suggested over the years. Here, we explore richness patterns of fern species in Xizang on the southern and western Tibetan Plateau along an elevational gradient (100–5300 m a.s.l.) and evaluate climatic factors causing the spatial decrease and increase of fern species richness. We used regression and correlation analyses to relate the species richness with elevation and climatic variables. Throughout our research, we identified 441 fern species from 97 genera and 30 families. The Dryopteridaceae family (S = 97) has the highest number of species. All energy-temperature and moisture variables except drought index (DI) had a significant correlation with elevation. The altitude has a unimodal relationship with fern species, and the species richness is the largest at an altitude of 2500 m. The horizontal richness pattern of fern species on the Tibetan Plateau also showed that areas of extremely high species richness are mainly distributed in Zayü and Mêdog County, with an average elevation of 2800 m and 2500 m, respectively. The richness of fern species has a log-linear relationship with moisture-related factors such as moisture index (MI), mean annual precipitation (MAP), and drought index (DI). Because the peak corresponds spatially with the MI index, the unimodal patterns confirm the significance of moisture on fern distributions. Our results showed that mid-altitudes have the highest species richness (high MI), but high elevations have lower richness due to high solar radiation, and low elevations have lower richness due to high temperatures and low precipitation. Twenty-two of the total species are classified as nearly threatened, vulnerable or critically endangered, and varied in elevation from 800 m to 4200 m. Such relationships between the distribution and richness of fern species and climates on the Tibetan Plateau can provide data support for future predictions of the impacts of climate change scenarios on fern species, the ecological protection of representative fern species, and references for the planning and construction of nature reserves in the future.