An increase in the carbapenem-hydrolyzing capacity of class Dβ-lactamase has been observed in strains of multiple species, posing a significant challenge to the control of antibiotic resistance. In this study, we aimed to investigate the genetic diversity and phylogenetic characteristics of newblaOXA-48-like variants derived fromShewanella xiamenensis. Three ertapenem-non-susceptibleS. xiamenensisstrains were identified, one isolated from the blood sample of an inpatient, the other two isolated from the aquatic environment. Phenotypic characterization confirmed that the strains were carbapenemase producers and exhibited antimicrobial resistance patterns to ertapenem, with some showing lower susceptibility to imipenem, chloramphenicol, ciprofloxacin, and tetracycline. No significant resistance to cephalosporins was observed. Sequence analysis revealed that one strain harboredblaOXA-181and the other two strains harboredblaOXA-48-like genes, with open reading frame (ORF) similarities withblaOXA-48ranging from 98.49% to 99.62%. The two novelblaOXA-48-like genes, namedblaOXA-1038andblaOXA-1039, respectively, were cloned and expressed inE. coli. The three OXA-48-like enzymes demonstrated significant hydrolysis activity against meropenem, and the classicalβ-lactamase inhibitor had no significant inhibitory effect. In conclusion, this study demonstrated the diversity of theblaOXAgene and highlighted the emergence of novel OXA carbapenemases inS. xiamenensis. Further attention toS. xiamenensisand OXA carbapenemases is recommended for the effective prevention and control of antibiotic-resistant bacteria.