AbstractClubroot is a soil-borne disease in cabbage (Brassica oleraceaL. var.capitataL.) caused byPlasmodiophora brassicae, which poses a great threat to cabbage production. However, clubroot resistance (CR) genes inBrassica rapacould be introduced into the cabbage via breeding to make it clubroot resistant. In this study, CR genes fromB. rapawere introduced into the cabbage genome and the mechanism of gene introgression was explored. Two methods were used to create CR materials: (i) The fertility of CR Ogura CMS cabbage germplasms containingCRawas restored by using an Ogura CMS restorer. After cytoplasmic replacement and microspore culture,CRa-positive microspore individuals were obtained. (ii) Distant hybridization was performed between cabbage andB. rapa,which contained three CR genes (CRa, CRb, andPb8.1). Finally, BC2individuals containing all three CR genes were obtained. Inoculation results showed that bothCRa-positive microspore individuals and BC2individuals containing three CR genes were resistant to race 4 ofP. brassicae. Sequencing results fromCRa-positive microspore individuals with specific molecular markers and genome-wide association study (GWAS) showed penetration at the homologous position of the cabbage genome by a 3.42 MbCRacontaining a fragment fromB. rapa; indicating homoeologous exchange (HE) as the theoretical basis for the introgression of CR resistance. The successful introduction of CR into the cabbage genome in the present study can provide useful clues for creating introgression lines within other species of interest.