Collagen mutations are commonly used in the creation ofCaenorhabditis eleganstransgenic strains, but their secondary effects are not fully characterized.We compared the mitochondrial function of N2,dpy-10, rol-6,and PE255C. elegans. N2 worms exhibited ~2-fold greater volume, mitochondrial DNA copy number, and nuclear DNA copy number than collagen mutants (p<0.05). Whole-worm respirometry and ATP levels were higher in N2 worms, but differences in respirometry largely disappeared after normalization to mitochondrial DNA copy number. This data suggests thatrol-6anddpy-10mutants are developmentally delayed but have comparable mitochondrial function to N2 worms once the data is normalized to developmental stage.