This study aims to describe and compare cardiopulmonary and subjective responses during high-intensity interval training with elastic resistance (EL-HIIT) and traditional high-intensity interval training (HIIT) sessions. Twenty-two healthy adults (27.6 ± 4.4 years) performed an EL-HIIT and a HIIT protocol consisting of 10 × 1 min at ~85%V·O2maxprescribed by cardiopulmonary-specific tests. Pulmonary parameters, heart rate (HR), blood lactate, and rate of perceived exertion (RPE) were measured during exercise. Pairedt-test and Cohen’sdeffect size were used to compare peak and average values. Two-way repeated measures ANOVA and mixed model with Bonferroni’s post hoc test were used to compare each bout during the session. The EL-HIIT session showed higher peak and average values of HR, ventilation (V·E), relative and absolute oxygen uptake (V·O2), carbon dioxide production (V·CO2), and RPE than HIIT (p< 0.05). The effect size (ES) comparing HIIT and EL-HIIT was large for HR,V·E, and lactate (≥0.8) in peak values. Comparing each bout between HIIT and EL-HIIT, no difference was found in peak values (p> 0.05) during the session (excluding baseline, warm-up, and recovery). EL-HIIT presented a more pronounced cardiopulmonary and subjective response than HIIT.