ABSTRACTHere, we report 7 metagenome-assembled genomes (MAGs) isolated from acid mine drainage sites in the eastern United States. Three genomes areArchaea, including two from the phylumThermoproteotaand one fromEuryarchaeota. Four genomes are bacterial, with one from the phylumCandidatusEremiobacteraeota (formerly WPS-2), one fromAcidimicrobiales(Actinobacteria), and two fromGallionellaceae(Proteobacteria).