AbstractTo explore the role of NAC transcription factors in mung bean (Vigna ratiata), we here comprehensively analyzedVrNAC13structure and expression patterns in the mung bean cultivar “Yulin No.1”. The nucleotide sequence ofVrNAC13(GenBank accession number xp014518431.1) was determined by cloning and sequencing the gene. A predicted transcriptional activation domain inVrNAC13was validated with a yeast one-hybrid assay. The composition and functional characteristics ofVrNAC13were analyzed using basic bioinformatics techniques, and the expression characteristics ofVrNAC13were analyzed via quantitative reverse transcription-PCR. The results showed thatVrNAC13was 1,068 bp in length and encoded a product of 355 amino acids.VrNAC13was predicted to contain a NAM domain and to belong to the NAC transcription factor family. The protein was hydrophilic and contained several threonine phosphorylation sites. Phylogenetic analysis showed thatVrNAC13was highly similar in sequence to twoArabidopsis thalianaNAC proteins; we hypothesize thatVrNAC13may perform functions in mung bean similar to those of the two closely related proteins inArabidopsis. Promoter analysis ofVrNAC13revealedcis-acting elements predicted to respond to abscisic acid (ABA), gibberellin, auxin, light, drought, low temperature, and other stressors.VrNAC13was most highly expressed in the leaves and expressed at very low levels in the stem and root. It was experimentally determined to be induced by drought and ABA. Based on these results,VrNAC13appears to regulate stress resistance in mung bean.