Simple SummaryThis article discusses the isolation of respirovirus 3 from a sample of femoral bone marrow from a wild boar carcass imported from Australia. The complete genome sequence was determined, and based on our results, the wild boar isolate may result from cross-species infection of wild boars with BRV3. The application of NGS techniques has allowed us to investigate and characterise the genome of this strain, which was initially isolated in 2004.AbstractParamyxoviruses are important pathogens affecting various animals, including humans. In this study, we identified a paramyxovirus in 2004 (180608_2004), isolated from a sample of the femoral marrow bone of a wild boar carcass imported from Australia. Antigenic and morphological characteristics indicated that this virus was similar to members of the familyParamyxoviridae. The complete genome phylogenetic analysis grouped this virus into genotype A of bovine parainfluenza virus type 3 (BPIV-3), recently renamed bovine respirovirus type 3 (BRV3), which also includes two swine paramyxoviruses (SPMV)—Texas-81 and ISU-92—isolated from encephalitic pigs in the United States in 1982 and 1992, respectively. The wild boar 180608_2004 strain was more closely related to both the BRV3 shipping fever (SF) strain and the SPMV Texas-81 strain at the nucleotide and amino acid levels than the SPMV ISU-92 strain. The high sequence identity to BRV3 suggested that this virus can be transferred from cattle to wild boars. The potential for cross-species transmission in theRespirovirusgenus makes it essential for intensified genomic surveillance.