Simple SummarySatyrization, a form of sexual interaction between males of one species with females of another species, has attracted renewed interest in pest management strategies. By inducing fitness costs in one or both interacting species, satyrization may indeed dramatically affect population dynamics, being a valuable tool to be used alone, or in conjunction with other area-wide control approaches. Here, we aimed to investigate the potential use of satyrization to control the invasive pestDrosophila suzukiiby usingD. melanogastermales. By realizing courtship tests, spermathecae analysis, and multiple-choice experiments, we showed thatD. melanogastermales were able to successfully court, mate and reduce the offspring ofD. suzukiifemales. These results, overall, showed that the use ofD. melanogastermales can be an effective tool to controlD. suzukiiand lay promising foundations for testing the application of this approach in field conditions.AbstractDrosophila suzukiirepresents one of the major agricultural pests worldwide. The identification of safety and long-lasting tools to suppress its populations is therefore crucial to mitigate the environmental and economic damages due to its occurrence. Here, we explore the possibility of using satyrization as a tool to control the abundance ofD. suzukii. By using males ofD. melanogaster, we realized courtship tests, spermathecae analysis, and multiple-choice experiments to assess the occurrence and extent of pre- and post-zygotic isolation between the two species, as well as the occurrence of fitness costs inD. suzukiifemales due to satyrization. Our results showed that: (i)D. melanogastermales successfully courtedD. suzukiifemales; (ii)D. melanogastermales significantly affected the total courtship time ofD. suzukiimales, which reduced from 22.6% to 6.4%; (iii)D. melanogastermales were able to inseminateD. suzukiiand reduce their offspring, inducing a high fitness cost. Reproductive interference occurs at different steps betweenD. melanogasterandD. suzukii, both alone and in combination with other area-wide control approaches.