Simple SummaryThe occurrence of cross-contamination of food in household kitchens could lead to severe foodborne diseases, so safe food practices are imperative. The study of the effectiveness and durability of a commercial quaternary ammonium compound-based surface coating regarding its antimicrobial efficacy, killing contact time, durability, and cytotoxicity was the objective of this study. Despite not showing cytotoxicity (if trace amounts of ≤0.2 mg/kg migrate into food when in contact with the surface) and being effective against three important pathogens in three artificially contaminated surfaces, its durability was less than one week on all surfaces cleaned in the usual manner. The regular use of quaternary ammonium compound-based surface coatings may significantly reduce surface contamination and increased food safety in domestic kitchens.AbstractFoodborne diseases are of major concern as they have a significant impact on public health, both socially and economically. The occurrence of cross-contamination of food in household kitchens is a serious threat and the adoption of safe food practices is of paramount importance. This work aimed to study the effectiveness and durability of a commercial quaternary ammonium compound-based surface coating which, according to the manufacturer, retains its antimicrobial activity for 30 days, and is suitable for all types of hard surfaces for the prevention and/or control of cross-contamination. For that, its antimicrobial efficacy, killing contact time and durability on three different surfaces—polyvinyl chloride, glass, and stainless-steel—against three pathogens—Escherichia coliATCC 25922,Acinetobacter baumanniiESB260 andListeria monocytogenesScott A—were tested according to the current antimicrobial treated surfaces efficacy test (ISO22196:2011). The results showed that the antimicrobial coating was effective against all pathogens with a reduction of >5.0 log CFU/cm2in less than one minute for the three surfaces, but its durability was less than one week on all surfaces cleaned in the usual manner. Additionally, trace amounts (≤0.2 mg/kg) of the antimicrobial coating, which may migrate into food when contacting the surface, did not show cytotoxicity to human colorectal adenocarcinoma cells. The suggested antimicrobial coating has the potential to significantly reduce surface contamination, ensure surface disinfection and reduce the likelihood of cross-contamination in domestic kitchens, although it is less durable than suggested. The use of this technology in household settings is an attractive complement to the existing cleaning protocols and solutions that are already in place.