AbstractThe complete mitochondrial genomes of twoProphantisspecies in the tribe Trichaeini (Lepidoptera: Crambidae) were sequenced using high‐throughput sequencing technology. They were assembled and annotated: The complete mitogenomes ofP. octoguttalisandP. adustawere 15,197 and 15,714 bp, respectively, and contain 13 protein‐coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and an A + T‐rich region. Their arrangement was consistent with the first sequenced mitogenome ofBombyx mori(Bombycidae) in Lepidoptera, which had thetrnM–trnI–trnQrearrangement. The nucleotide composition was obviously AT‐biased, and all PCGs, except for thecox1gene (CGA), used ATN as the start codon. Except fortrnS1, which lacked the DHU stem, all tRNA genes could fold into the clover‐leaf structure. The features of these two mitogenomes were highly consistent with those of other species of Spilomelinae in previous studies. Phylogenetic trees of Crambidae were reconstructed based on mitogenomic data using maximum likelihood and Bayesian inference analysis methods. Results showed that Trichaeini in this study robustly constitute a monophyletic group in Spilomelinae, with the relationships (Trichaeini + Nomophilini) + ((Spilomelini + (Hymeniini + Agroterini)) + Margaroniini). However, the affinities of the six subfamilies Acentropinae, Crambinae, Glaphyriinae, Odontiinae, Schoenobiinae, and Scopariinae within the “non‐PS Clade” in Crambidae remained doubtful with unstable topologies or low supports.