Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is induced through the salicylic acid (SA)-mediated signaling pathway. Here, we characterized 3-chloro-1-methyl-1H-pyrazole-5-carboxylic acid (CMPA) as an effective SAR inducer inArabidopsis. The soil drench application of CMPA enhanced a broad range of disease resistance against the bacterial pathogenPseudomonas syringaeand fungal pathogensColletotrichum higginsianumandBotrytis cinereainArabidopsis, whereas CMPA did not show antibacterial activity. Foliar spraying with CMPA induced the expression of SA-responsible genes such asPR1,PR2andPR5. The effects of CMPA on resistance against the bacterial pathogen and the expression ofPRgenes were observed in the SA biosynthesis mutant, however, while they were not observed in the SA-receptor-deficientnpr1mutant. Thus, these findings indicate that CMPA induces SAR by triggering the downstream signaling of SA biosynthesis in the SA-mediated signaling pathway.