﻿AbstractSpecies ofDiaporthehave been reported as plant endophytes, pathogens and saprobes on a wide range of plant hosts. Strains ofDiaporthewere isolated from leaf spots ofSmilaxglabraand dead culms ofXanthiumstrumariumin China, and identified based on morphology and molecular phylogenetic analyses of combined internal transcribed spacer region (ITS), calmodulin (cal), histone H3 (his3), translation elongation factor 1-alpha (tef1) and β-tubulin (tub2) loci. As a result, two new species namedDiaportherizhaoensisandD.smilacicolaare identified, described and illustrated in the present study.