In recent years, with the widespread use of TiO2–GO nanocomposite in industry, especially in the remediation of water environments, its toxic effects on aquatic organisms have received increasing attention. As molting is extremely important for crustaceans in their growth, in this study, we cloned the full-length cDNA sequences of two key genes related to molting,nuclear hormone receptor E75(E75) andnuclear hormone receptor HR3(HR3), inMacrobrachium rosenbergii, examined the gene expression profile, and investigated their toxicological effects on crustacean molting through nanomaterial exposure. The amino acid sequences for E75 and HR3 were respectively determined to encode 1138 and 363 acid residues. Sequence analysis showed that both E75 and HR3 contain a HOLI domain, with theE75ofM. rosenbergiibeing more closely related to theE75ofPalaemon carinicauda. These two genes were expressed at the highest levels in muscle, followed by hepatopancreas. The results showed that the expressions ofE75andHR3in hepatopancreas and muscle tissues were significantly decreased after exposure to 0.1 mg/L of TiO2–GO composite nanoparticles (P< 0.05). This study will serve as a foundation for subsequent research into the evaluation of nanomaterial toxicity on crustacean species.