The high isomeric
complexity of glycans makes them particularly
difficult to analyze. While ultra-high-resolution ion mobility spectrometry
(IMS) can offer rapid baseline separation of many glycan isomers,
their unambiguous identification remains a challenging task. One approach
to solving this problem is to identify mobility-separated isomers
by measuring their highly resolved cryogenic vibrational spectra.
To be able to apply this approach to complex mixtures at high throughput,
we have recently developed a Hadamard transform multiplexed spectroscopic
technique that allows measuring vibrational spectra of all species
separated in both IMS and mass spectrometry dimensions in a single
laser scan. In the current work, we further develop the multiplexing
technique using ion traps incorporated directly into the IMS device
based on structures for lossless ion manipulations (SLIM). We also
show that multiplexed spectroscopy using perfect sequence matrices
can outperform standard multiplexing using Simplex matrices. Lastly,
we show that we can increase the measurement speed and throughput
further by running multiple multiplexing schemes using several SLIM
ion traps in combination with simultaneous spectroscopic measurements
in the segmented cryogenic ion trap.