Carrion´s disease is caused byBartonella bacilliformis, it is a Gram-negative pleomorphic bacterium.B.bacilliformisis transmitted byLutzomyia verrucarumin endemic areas of the Peruvian Inter-Andean valleys. Additionally, the pathogenicity ofB.bacilliformisinvolves an initial infection of erythrocytes and the further infection of endothelial cells, which mainly affects children and expectant women from extreme poverty rural areas. Therefore, the implementation of serological diagnostic methods and the development of candidate vaccines for the control of CD could be facilitated by the prediction of linear b-cell epitopes in specific proteins ofB.bacilliformisby bioinformatics analysis. In this study, We used anin-silicoanalysis employing six web servers for the identification of epitopes in proteins ofB.bacilliformis. The selection ofB.bacilliformis-specific proteins and their analysis to identify epitopes allowed the selection of seven protein candidates that are expected to have high antigenic activity.