A subject of great interest is the bioprospecting of microorganisms and their bioactive byproducts, such as pigments. Microbial pigments have various benefits, including being safe to use due to their natural makeup, having therapeutic effects, and being produced all year round, regardless of the weather or location.Pseudomonas aeruginosaproduces phenazine pigments that are crucial for interactions betweenPseudomonasspecies and other living things. Pyocyanin pigment, which is synthesized by 90–95% ofP. aeruginosa, has potent antibacterial, antioxidant, and anticancer properties. Herein, we will concentrate on the production and extraction of pyocyanin pigment and its biological use in different areas of biotechnology, engineering, and biology.