ObjectiveGanodermaextracts have the potential to be used as anti-cancer, anti-inflammatory, immunomodulator, and antimicrobial agents, as evaluated in numerous studies. This study was aimed to determine the lethal and inhibitory effects of aqueous, hydroalcoholic, and alcoholic extracts ofGanoderma lucidumonToxoplasma gondiiRH strain tachyzoites,in vitro.ResultsAll three types of extracts showed toxoplasmacidal effects. The highest percentage of mortality was related to hydroalcoholic extract. The EC50 ofGanodermaextracts for tachyzoites were 76.32, 3.274, and 40.18 for aqueous, hydroalcoholic and alcoholic extracts, respectively. The selectivity index obtained for hydroalcoholic extract was 71.22, showing the highest activity compared to other extracts. According to our findings, the hydroalcoholic part was the most effective substance among the extracts. This basic study showed obvious anti-toxoplasma effect ofGanoderma lucidumextracts. These extracts can be used as candidates for further in-depth and comprehensive studies especiallyIn vivoexperiments to prevent toxoplasmosis.