BackgroundAcute rhinosinusitis (ARS) is one of the common diseases of upper respiratory tract infection in children. Bacterial infection is a significant aggravating factor in pediatric ARS. In this research, our goal was to detected the bacterial flora and antibiotic sensitivity of ARS in Chinese children.MethodsWe recruited 133 children with ARS between January 2020 and January 2022 from our hospital. Sinus secretion were collected and cultured for Gram stain as well as antimicrobial susceptibility tests.ResultsMoraxella catarrhalis,Staphylococcus aureus,Haemophilus influenzae,Streptococcus pneumoniaeandPseudomonas aeruginosawere detected in order in children with ARS, of which 25% were negative for bacterial culture and 10% were positive for two strains. Amoxicillin and clavulanate potassium were useful forHaemophilus influenzae,Streptococcus pneumoniaeandMoraxella catarrhalis. Quinolones are useful forStaphylococcus aureus,Haemophilus influenzae,Streptococcus pneumoniaeandPseudomonas aeruginosa.ConclusionsThis research updates the proportion of ARS bacterial infection in children in southern China and the antibiotic sensitivity.