Background and Objective: The role of the pharmacist in healthcare society is unique, since they are providers of health information and medication counseling to patients. Hence, this study aimed to evaluate Awareness, Perceptions, and Opinions towards Artificial intelligence (AI) among pharmacy undergraduate students at King Saud University (KSU), Riyadh, Saudi Arabia.Materials and Methods:A cross-sectional, questionnaire-based study was conducted between December 2022 and January 2023 using online questionnaires. The data collection was carried out using convenience sampling methods among senior pharmacy students at the College of Pharmacy, King Saud University. Statistical Package for the Social Sciences version 26 was used to analyze the data (SPSS).Results: A total of one hundred and fifty-seven pharmacy students completed the questionnaires. Of these, most of them (n= 118; 75.2%) were males. About 42%, (n= 65) were in their fourth year of study. Most of the students (n= 116; 73.9%) knew about AI. In addition, 69.4% (n= 109) of the students thought that AI is a tool that helps healthcare professionals (HCP). However, more than half 57.3% (n= 90) of the students were aware that AI would assist healthcare professionals in becoming better with the widespread use of AI. Furthermore, 75.1% of the students agreed that AI reduces errors in medical practice. The mean positive perception score was 29.8 (SD = 9.63; range-0–38). The mean score was significantly associated with age (p= 0.030), year of study(p= 0.040), and nationality (p= 0.013). The gender of the participants was found to have no significant association with the mean positive perception score (p= 0.916).Conclusions: Overall, pharmacy students showed good awareness of AI in Saudi Arabia. Moreover, the majority of the students had positive perceptions about the concepts, benefits, and implementation of AI. Moreover, most students indicated that there is a need for more education and training in the field of AI. Consequently, early exposure to content related to AI in the curriculum of pharmacy is an important step to help in the wide use of these technologies in the graduates’ future careers.