AbstractPresent study was carried out to investigate the variation in web architecture ofLarinia chloris(Audouin 1826) in relation to seasonal differences and the biotic factors of the environment. In addition, relative abundance, behavior, and predatory potential ofL. chloriswere also recorded. For this purpose, 100 orb-webs ofL. chloriswere observed in rice fields (August–October, 2022) from 3 districts of Punjab (Lahore, Sheikhupura, and Kasur). Percent abundance ofL. chloriswas found to be highest in rice fields from Barki road, Lahore (39.53%). All the webs ofL. chloriswere vertical at height equal to the height of vegetation (115.2 ± 9.7 cm). Time required to complete the web was 45 ± 5 min. There was positive correlation between web architecture and vegetation height. Web capture area and average mesh height ofL. chlorisalso showed positive correlation with carapace length. There was a significant difference in various web parameters (number of spirals, number of radii, capture area, average mesh height, upper radii, lower radii, left radii, and right radii) among different trapping months. A total of 1,326 insects were recorded from the 100 webs ofL. chloris. The prey abundance was found to be highest in the fields from Barki Road, Lahore. The majority of the prey collected from webs ofL. chlorisbelonged to order Diptera, Hemiptera, Coleoptera, and Lepidoptera. However, prey items recorded during different growth stages (from vegetative to ripening) varied significantly. This is the first ever report describing the ecology ofL. chlorisin rice fields from Punjab, Pakistan.