AbstractMongolian butter and Tude are traditional high‐fat dairy products produced in Xilin Gol, China, which have unique chemical and microbiological characteristics. Mongolian Tude is made from Mongolian butter, dreg, and flour. In this study, the traditional manufacturing process of Mongolian butter and Tude was investigated for the first time. Mongolian butter was characterized by high‐fat content (99.38 ± 0.63%) and high acidity (77.09 ± 52.91°T), whereas Mongolian Tude was considered a high‐fat (21.45 ± 1.23%) and high‐protein (8.28 ± 0.65%) dairy product obtained by butter, dreg, and flour. Mongolian butter and Tude were proven to be safe for human consumption in terms of benzopyrene content. In addition,Listeria monocytogenes,Staphylococcus aureus,Salmonella, coliforms, and aflatoxin M1 were not detected in the samples. Bacteria and molds were not isolated from Mongolian butter; in contrast, the total count of bacteria and molds in Mongolian Tude was within the range of 4.5 × 102to 9.5 × 104and 0 to 2.2 × 105, respectively. Moreover,Lactococcus(41.55%),Lactobacillus(11.05%),Zygosaccharomyces(40.20%), andPichia(12.90%) were the predominant bacterial and fungal genera, andLactobacillus helveticus(15.6%),Lactococcus raffinolactis(9.6%),Streptococcus salivarius(8.5%),Pantoea vagans(6.1%),Bacillus subtilis(4.2%),Kocuria rhizophila(3.5%),Acinetobacter johnsonii(3.5%),Zygosaccharomyces rouxii(46.2%),Pichia fermentans(14.7%), andDipodascus geotrichum(11.7%) were the predominant species in the microbiota of Mongolian Tude. Thus, it can be stated that the microbiota of food products produced by different small families varied significantly. Collectively, the findings presented herein are the first report of chemical and microbiological characterization of products of geographical origin and highlight the need for standardization of manufacturing procedures of Mongolian butter and Tude in the future.