SomeOphiocordycepsspecies infecting ants are able to manipulate the host behavior. The hosts are manipulated in order to move to location that are advantageous for fungal spore transmission.Ophiocordycepsspecies that are able to manipulate the ant's behavior are called "zombie-ant fungi". They are widespread within tropical forests worldwide, with relatively few reports from subtropical monsoon evergreen broad-leaf forest. Zombie-ant fungi have been described and reported in different countries worldwide. However, there were a few reports from China. This study proposed six new species of zombie-ant fungi from China based on multi-gene (SSU, LSU,TEF,RPB1andRPB2) phylogenetic analyses and morphological characteristics. Six novel species ofOphiocordycepsfrom China were identified as theOphiocordyceps unilateraliscore clade, forming a separate lineage with other species. Six novel species ofOphiocordycepswith hirsutella-like asexual morphs exclusively infecting ants were presented herein, namely,Ophiocordyceps acroasca,Ophiocordyceps bifertilis,Ophiocordyceps subtiliphialida,Ophiocordyceps basiasca,Ophiocordyceps nuozhaduensisandOphiocordyceps contiispora. Descriptions and illustrations for six taxon were provided. Five of these species were collected from the subtropical monsoon evergreen broad-leaf forest, and one was collected from the rainforest and subtropical monsoon evergreen broad-leaf forest. This work proposes that the same host ofCamponotuscan be infected by multiple ant pathogenic fungi, while multiple ants ofPolyrhachiscan be infected by the same pathogenic fungi at the same time. This study contributes towards a better understanding of the evolutionary relationship between hosts and fungi, and provides novel insights into the morphology, distribution, parasitism, and ecology ofOphiocordyceps unilateralissensu lato. We have provided a method for obtaining living cultures ofOphiocordyceps unilateraliscomplex species and their asexual morphs based on the living cultures, which is of significant value for further studies ofOphiocordyceps unilateraliscomplex species in the future.