The aim of this study was to estimate the occurrence ofBartonellaspp. per household in cats and the risk factors forBartonellaspp. positivity in cats and their owners from Valdivia, Chile. A total of 464 cats (distributed within 324 households) and 326 humans (control group [n= 112] and cat owner [n= 214]) distributed in 262 households were sampled. From the cat owners (n = 214), 128 humans were in households where the cat was also sampled, totaling 84 households with dual sampling. Real-time PCR (qPCR) was used forBartonellaspp. detection in blood from cats and humans, and immunofluorescent immunoassay (IFA) anti-Bartonella henselaewas performed in human serum samples. Out of the total of 324 households, 20.43% presented at least oneBartonellapositive cat. From the households with dual sampling, 29.7% (25/84) presented at least one qPCR-Bartonellaspp. positive cat. However,BartonellaDNA was not amplified in humans, and in 7.3% (6/82) of the households was found at least one of the cat's owners exposed toB. henselae. Cats younger than one year (Odds Ratio (OR) = 5.3), non-neutered (OR 3.46), sampled at home (OR 5.82), and with improper application of tick/flea control products (OR 3.13) showed a higher risk forBartonellaspp. presence. Humans with occupational exposure involving animal contact, were more likely to exhibitB. henselaeseropositivity (OR 7.5).Bartonellaspp. was present in the cats a moderate number of households, butBartonellaDNA was not detected in owners' blood, inferring that there is a low risk of recent human infection in the studied population.