ABSTRACTRhizogenicAgrobacteriumbiovar 1 strains are important plant pathogens that cause hairy root disease inCucurbitaceaeandSolanaceaecrops cultivated under hydroponic conditions. In contrast to tumorigenic agrobacteria, only a few genome sequences of rhizogenic agrobacteria are currently available. Here, we report the draft genome sequences of 27 rhizogenicAgrobacteriumstrains.