Graphene oxide (GO) has attracted attention as an excellent membrane material for water treatment and desalination owing to its high mechanical strength, hydrophilicity, and permeability. In this study, composite membranes were prepared by coating GO on various polymeric porous substrates (polyethersulfone, cellulose ester, and polytetrafluoroethylene) using suction filtration and casting methods. The composite membranes were used for dehumidification, that is, water vapor separation in the gas phase. GO layers were successfully prepared via filtration rather than casting, irrespective of the type of polymeric substrate used. The dehumidification composite membranes with a GO layer thickness of less than 100 nm showed a water permeance greater than 1.0 × 10−6mol/(m2s Pa) and a H2O/N2separation factor higher than 104at 25 °C and 90–100% humidity. The GO composite membranes were fabricated in a reproducible manner and showed stable performance as a function of time. Furthermore, the membranes maintained high permeance and selectivity at 80°C, indicating that it is useful as a water vapor separation membrane.