ObjectiveThe aim of this research was to investigate the use ofStichopus herrmanninanoparticle gel on the ulcer healing process by observing blood vessels, fibroblasts, and Collagen type-I (COL-1) expression on the 4 and 7th days after trauma.Materials and MethodsGold sea cucumber (Stichopus herrmanni) powder was processed by freeze-drying method, then by high-energy milling to form nanoparticle size, and then with CMC 2% to make hydrogel. Traumatic ulcers were formed by induction using a burner. Five groups of male Wistar rats, each consisting of six tails, were divided into a negative control group that was given a placebo, the positive control group was given 0.2% hyaluronic acid, and the treatment group was given gold sea cucumbers with concentrations of 0.135, 0.27, and 0.54% (SH1-SH2-SH3). Fibroblast and blood vessels were examined with hematoxylin-eosin on day 3 and 7, while COL-1 expression was examined with immunohistochemistry on day 7. The rats' mucosa was taken on the 3rd and 7th days after the traumatic ulcer was formed.Statistical AnalysisThe data were analyzed using a one-way analysis of variance followed by a post-hoc test with apless than 0.05.ResultsNanoparticles gel freeze-drying ofStichopus herrmanniincreased blood vessels on day 3. Angiogenesis continued to occur, which resulted in increased fibroblast and COL-1 expression on day 7.ConclusionsThe application ofStichopus herrmanninanoparticle gel at 0.27% effectively increased the number of blood vessels, fibroblasts, and COL-1 expression in healing traumatic ulcers.