Livistonais a genus of family Arecaceae and widely grown in tropical areas. The phytochemical analysis of the leaves and fruits of twoLivistonaspecies,L. chinensisandL. australiswere carried out using UPLC/MS and determination of the total phenolic and total flavonoid contents, in addition to the isolation and identification of five phenolic compounds and one fatty acid fromL. australisfruits. The total phenolic compounds varied from 19.72 to 78.87 mg GAE g−1dry plant, while the total flavonoid contents were in the range of 4.82–17.75 mg RE g−1dry plant. The UPLC/MS analysis of the two species led to the characterization of forty-four metabolites belonging mainly to the different classes of flavonoids and phenolic acids, while the compounds isolated fromL. australisfruits were identified as gallic acid, vanillic acid, protocatechuic acid, hyperoside, quercetin 3-O-α-d-arabinopyranoside and dodecanoic acid. Thein vitrobiological evaluation ofL. australisleaves and fruits were estimated as anticholinesterase, telomerase reverse transcriptase (TERT) potentiation and anti-diabetic through measuring the capacity of the extracts to inhibit dipeptidyl peptidase (DPP-IV). The results revealed that the leaves showed remarkable anticholinesterase and antidiabetic activity compared to fruits with IC50values of 65.55 ± 3.75 ng mL−1and 90.8 ± 4.48 ng mL−1, respectively. In the TERT enzyme assay, the leaves extract triggered a 1.49-fold increase in telomerase activity. This work showed that theLivistonaspecies are a good source for flavonoids and phenolics, which play an important role in anti-aging and the treatment of chronic diseases, such as diabetes and Alzheimer's.