AbstractBackgroundApolipoprotein E (ApoE) is associated with Alzheimer’s disease (AD) and cognitive dysfunction in elderly individuals. There have been extensive studies on behavioral abnormalities in ApoE-deficient (Apoeshl) mice, which have been described as AD mouse models. Spontaneously hyperlipidemic mice were discovered in 1999 as ApoE-deficient mice due to ApoE gene mutations. However, behavioral abnormalities in commercially available Apoeshlmice remain unclear. Accordingly, we aimed to investigate the behavioral abnormalities of Apoeshlmice.ResultsApoeshlmice showed decreased motor skill learning and increased anxiety-like behavior toward heights. Apoeshlmice did not show abnormal behavior in the Y-maze test, open-field test, light/dark transition test, and passive avoidance test.ConclusionOur findings suggest the utility of Apoeshlmice in investigating the function of ApoE in the central nervous system.