AbstractOne hundred and twenty-one Angus × SimAngus-crossbred steers (body weight (BW) = 159 ± 22 kg) were used to evaluate the effects of different grain inclusion (GI) rates in diets provided to early-weaned calves and steroidal implants (SI) utilization on growth performance and carcass characteristics, particularly intramuscular fat deposition, of beef steers. The experiment was conducted as a randomized complete block design with a 2 × 2 factorial arrangement of treatments, consisting of two GI rates (35% vs. 58%, dry matter (DM) basis), each one associated or not to steroidal implant utilization (no implants vs. 80 mg trenbolone acetate (TA) + 16 mg estradiol followed by 120 mg TA + 24 mg of estradiol). After being early-weaned (124 ± 14 d of age), steers were offered an average of 4.5 kg/d (DM basis) of a concentrate-based diet with a greater or lesser GI rate for 60 d. After being fed a concentrate-based diet with different GI rates for 60 d, steers were fed a common backgrounding diet for 56 d and subsequently fed a common high-grain diet until harvested at a constant final BW (620 kg). Steers were not implanted until the beginning of the backgrounding phase and then re-implanted when initiating the finishing phase. Data were analyzed using PROC MIXED in SAS. There were no GI × SI interactions (P≥ 0.62) for any of the growth performance parameters throughout the experimental period. Implanted steers tended to have a greater average daily gain (P= 0.10) during the finishing phase than nonimplanted steers. For the 12th rib fat thickness and yield grade (YG), a GI × SI interaction (P= 0.03) and a tendency for a GI × SI interaction (P= 0.10) was detected, respectively. Nonimplanted steers fed diets with greater GI rates presented the greatest 12th rib fat thickness and tended to have the greatest YG among treatments. No other interactions (P≥ 0.33) were observed for the hot carcass weight,Longissimusmuscle (LM) area, quality grade, marbling score, and kidney-pelvic-heart fat content. Steers fed diets with lesser GI rates tended to have a greater LM area than steers fed diets with greater GI rates (P= 0.10). Results from this experiment indicate that varying GI rates in diets provided to early-weaned calves and subsequent implantation with steroidal hormones did not affect marbling deposition.