α(1,2)fucosyltransferase (Se enzyme) encoded byFUT2is involved in the secretor status of ABH(O) blood group antigens. Thesedel2allele is one of the non-functionalFUT2(se) alleles in which 9.3 kb, containing the entire coding region ofFUT2, is deleted byAlu-mediated nonhomologous recombination. In addition to this allele, three SNPs ofFUT2, c.375A>G, c.385A>T, and c.571C>T, appear to be prevalent in certain Oceanian populations such as Polynesians. Recently, we developed an endpoint genotyping assay to determinesedel2zygosity, using a FAM-labeled probe for detection of thesedel2allele and a VIC-labeled probe for the detection ofFUT2. In this study, instead of the VIC probe, a HEX-labeled probe covering both c.375A>G and c.385A>T and a Cy5-labeled probe covering c.571C>T were added to thesedel2allele assay mixture to allow for the simultaneous detection of these four variations via endpoint genotyping forsedel2zygosity and fluorescence melting curve analysis for c.375A>G, c.385A>T, and c.571C>T genotyping. The results obtained from 24 Samoan subjects using this method were identical to those obtained using previous methods. Therefore, it appears that the present method can accurately determine these four variations simultaneously.