BackgroundNursing students enter nursing programs with idealistic perceptions of what it is to be a nurse. Upon graduation, many find these perceptions mismatched with the actual nurse’s role. This can lead to discontentment in their chosen career. These issues highlight the importance of nursing students developing an understanding of the nurse’s role during their undergraduate nursing education. One way to accomplish this is to assess perceptions and address them accordingly during the nursing program. Survey tools assessing perceptions of nursing exist but lack contemporary and multicultural foci.AimTo develop a feasible, valid, and reliable survey tool to identify nursing students’ perceptions of being a nurse.Design/MethodsIn Phase 1, a literature review and Nominal Group Technique meetings were used to generate primary survey items. Phase 2 included a pre-pilot and online pilot testing of the Perceptions of being a Registered Nurse (PRN) survey tool with 797 nursing students across all year levels at three Australian Universities.ResultsThe 34-item PRN survey tool uses a five-point Likert scale to measure nursing students’ perceptions of nursing, including factors influencing a nurse’s well-being, attributes and qualities of nurses, the role of the nurse, and nursing professionalism. The Item-Content validity index was high (> 0.78), and the inter-item correlation validity was identified by Pearson’s product-moment coefficient ofr= .712. Internal reliability was confirmed with a Cronbach’s alpha = 0.83. Based upon the participation completion rate, the survey tool was deemed applicable and feasible. The majority of respondents believed that nurses have altruistic attributes; however, perceptions of nursing varied significantly when rating factors influencing the physical, emotional, and social well-being of a nurse. In later stages of training, respondents were more likely to agree that nursing is physically and emotionally demanding and that nurses experience social isolation due to shift work, finding it difficult to achieve a work-life balance.ConclusionsThe PRN survey tool was found to be valid, reliable, and feasible. Future use and outcomes from PRN assessments may lead to changes to nursing curricula that enhance nursing students’ perceptions of nursing.Supplementary InformationThe online version contains supplementary material available at 10.1186/s12912-023-01324-7.