The effect of short carbon fiber (SCF) filler on the mechanical properties of 3D-printed acrylonitrile butadiene styrene (ABS) was investigated. The fused filament fabrication (FFF) method was used for the manufacturing of samples. Elastic properties and strength characteristics of samples made of conventional ABS and SCF-reinforced ABS were compared in tensile and bending tests. Fracture toughness and critical strain energy release rate were also determined. In addition, 3D-printed monofilament SCF-reinforced samples were fabricated, the internal structure of which was analyzed using microcomputed tomography (micro-CT). Based on the tomography data, finite-element (FE) models of representative volume elements (RVEs) of the reinforced material were created and used for the numerical calculation of effective characteristics. Numerical and experimental results for the effective elastic properties were compared with the Mori-Tanaka homogenization technique. The ABS samples filled with SCF showed considerably higher mechanical characteristics than those of the conventional ABS. Finally, the dependence between the strength characteristics and elastic properties of the samples on the diameter of the nozzle used for 3D printing was established. 3D-printed ABS reinforced with SCF demonstrated a gain in tensile strength and fracture toughness by 30% and 20%, respectively. Interlayer adhesion strength in flexure tests showed an increase of 28% compared to pure ABS samples.