Clathrin is an evolutionarily highly conserved evolutionary protein consisting of clathrin light chains (CLC) and clathrin heavy chains (CHC), and these form its basic structure. Clathrin is an important host factor in the process of viral infection. In this study, we cloned theBcCLC1gene and theBcCLC2gene from the ‘49CX’ variety of non-heading Chinese cabbage (NHCC,Brassica campestrisL. ssp.chinensisMakino) and verified their functions. The results showed thatBcCLC1was mainly localized in the cytomembrane and cytoplasm, and only a small amount entered the nucleus.BcCLC2encoded a protein comprising 265 amino acids that were distributed in the cytomembrane, nucleus, and cytoplasm. A BiFC assay and yeast two-hybrid (Y2H) analysis showed thatBcCLCs(BcCLC1andBcCLC2) could interact with several TuMV proteins. We further investigated the mechanism ofBcCLCsin regulating TuMV virus infections in NHCC, and observed thatBcCLCsgene silencing inhibited TuMV infections and overexpression ofBcCLCsinArabidopsispromoted TuMV infections in NHCC. Finally, mutants ofArabidopsishomologs ofBcCLCswere also screened and subjected to TuMV inoculation tests. In conclusion, we speculate thatBcCLCsconferTurnip mosaic virus(TuMV) resistance in NHCC by interacting with TuMV proteins to promote the intracellular transport of the virus.