AbstractBackgroundThis study focused on providing a report about the current situation of AMR in Yastebsheron Hospital in Khartoum, Sudan, monitoring and identifying the resistant trends in clinical isolates, highlighting the gaps in the IPC and ASP through antibiogram analysis, and implementing its data to improve antibiogram-guided empirical antibiotic therapy selection.MethodA retrospective longitudinal study. The study retrieved data from microbiology laboratory records from 1 January 2022 to 31 December 2022, at Yastebsheron Hospital. A total of 1182 clinical specimens were processed in 2022.ResultsThe most frequent samples were urine, with a prevalence rate of 59.3%, of which 69.8% were female and 30.2% were male. The total Gram-positives were 52.6%, withStaphylococcus aureusaccounted for 98%; out of the total specimens, MRSA accounted for 48.6%, and MSSA accounted for 11.8%. The total Gram-negative isolates were 48.3%, of whichEscherichia coli Enterobacter,Proteus mirabilis,Pseudomonas aeruginosaandKlebsiella pneumoniaerepresented 25.4%, 21.8%, 9.8%, 9.4% and 3.8%, respectively. MSSA were highly susceptible to meropenem (97%) and vancomycin (95%), and luckily, MRSA isolates were susceptible to levofloxacin and colistin at 86% and to meropenem (84%), with minimal susceptibility to clindamycin (37%) and nalidixic acid (12%). Meropenem, colistin and nitrofurantoin were the most effective antibiotics againstE. coliwith more than 80% susceptibility rates, while cephalosporins and nalidixic acid showed minimal efficacy. Meropenem and levofloxacin were highly effective againstEnterobacter,P. mirabilis, while the susceptibility rates for second-, third- and fourth-generation cephalosporins were less than 50% for both of them, and less than 30% forP. aeruginosa,P. aeruginosawas 100% susceptible to colistin.ConclusionsAmong infections, UTIs had the highest incidence rates. The majority of the isolates wereS. aureus, then came Enterobacteriaceae, particularlyE. coli, and thenP. aeruginosa. Contrary to ciprofloxacin and norfloxacin, meropenem showed high efficacy against G-positive and G-negative isolates, while levofloxacin displayed moderately high results. Except for MSSA, the effectiveness of second-, third- and fourth-generation cephalosporins against G-positive and G-negative organisms was compromised. In order to stop the deterioration of the AMR status, it is crucial to implement the ASP strategies and tools effectively, as the emergence of MDR strains is alarming. Antibiogram’s formulation is essential for directing empirical antibiotic therapy and tracking the emergence of resistance.