ABSTRACTBackgroundGlass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system.MethodsHemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume andPlasmodium falciparuminfection intensity on mosquito guts.ResultsWhile mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane.ConclusionsHemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.