Tick-borne pathogens (TBPs), transmitted by the bites of ticks, are of great medical and veterinary importance. They include bacteria, viruses, and protozoan parasites. To provide fundamental data on the risk of tick contact and public health strategies, we aimed to perform a molecular investigation on four tick-borne bacterial pathogens in ticks collected from humans across the Republic of Korea (ROK) in 2021. In total, 117 ticks were collected, includingHaemaphysalis longicornis(56.4%),Amblyomma testudinarium(26.5%),Ixodes nipponensis(8.5%),H. flava(5.1%), andI. persulcatus(0.9%). Among the ticks, 20.5% (24/117) contained tick-borne bacterial pathogens, with infection rates of 17.9% forRickettsia(CandidatusRickettsia jingxinensis,R. tamurae,R. monacensis, andCandidatusRickettsia tarasevichiae), 2.5% forAnaplasma(A. phagocytophilum,A. capra, andA. bovis), and 0.9% forEhrlichia(Ehrlichiasp.). Additionally, the co-detection rate forR. monacensisandA. phagocytophilumwas 0.9%. To our knowledge, this is the first report ofA. capraandA. bovisdetection in ticks collected from humans in the ROK. This study contributes to the understanding of the potential risk of tick contact and provides fundamental data for establishing a public health strategy for tick-borne disease management in the ROK.