AbstractThis study aimed to investigate the effect of wormwood and rosemary supplementation on some reproductive traits of Barbarine rams. The experiment lasted 2 months. Twenty‐four adult rams were divided into four groups (n= 6) balanced for the weight (53.3 ± 1.2 kg body weight [BW] ± SD). All rams received 1200 g of straw and 600 g of barley. Control rams (C) without aromatic medicinal plant (AMP), while experimental rams received 20 g of fresh rosemary leaves (R), 20 g of fresh wormwood leaves (A), and 10 g of fresh rosemary leaves plus 10 g of fresh wormwood leaves (RA). The results revealed that the live weight of all rams increased (p< .05) in the RA group compared to the C, A, and R groups. Scrotal circumference increased in the R rams when compared to the controls rams (p< .05). For sperm parameters we showed that the A rams had higher sperm concentrations (p< .05). But, the sperm volume decreased in the R rams (p >.05). However, when the rams received rosemary plus wormwood, their sperm volume increased (p> .05). The sperm mass motility was higher for the A, R and AR rams in comparison to the C rams (p= .05). On the other hand, biochemical analysis of the seminal fluid showed no effect of diets on calcium and total proteins concentration. But the measurement of glucose and seminal insulin showed a decrease (p< .05) in these two biochemical markers in group A rams and a decrease (p< .05) in insulin without modification of the glucose concentration in R rams. Blood glucose and insulin decreased in the animals on AMP diet compared to the other groups (p< .05) while aspartate aminotransferase (AST) increased (p< .05). Rosemary leaves (R and RA groups) increased (p< .05) plasma cortisol compared to the other groups. It can be concluded that the addition ofRosmarinus officinalisand/orArtemisia herba albain ram diet can have a positive effect on the reproductive function by increasing the concentration and motility of sperm, plasma testosterone, and sexual behavior.