Flexible pressure sensors with high sensitivity have great potential applications in wearable electronics. However, it is still a great challenge to prepare sense electrodes with high flexibility, high sensitivity, and high electrochemical performance. Here, we propose a novel and simple method for carbonizing cotton fibers as excellent electrically conductive materials. Moreover, carbonized cotton fiber (CCF) and polydimethylsiloxane (PDMS) were assembled into a flexible sense electrode. The CCF/PDMS electrode shows a high sensitivity of 10.8 kPa−1, a wide response frequency from 0.2–2.0 Hz, and durability over 900 cycles. The combined CCF/PDMS sensors can monitor human movement and pulse vibration, showing the enormous potential for use in wearable device technology. Additionally, the CCF/PDMS can be used as electrodes with a specific capacitance of 332.5 mF cm−2at a current density of 5 mA cm−2, thanks to their high electrical conductivity and hydrophilicity, demonstrating the promising prospect of flexible supercapacitors.