This study aimed to obtain and investigate ZnCr2Se4single crystals doped with rhenium. The single crystals were obtained by applying chemical vapour transport. An X-ray study confirmed the cubic (Fd3¯m) structure of the tested crystals. Thermal, magnetic, electrical, and specific heat measurements accurately determined the physicochemical characteristics, which revealed that the obtained single crystals arep-type semiconductors with antiferromagnetic order below the Néel temperatureTN= 21.7 K. The Debye temperature had a value of 295 K. The substitution of Re-paramagnetic ions, possessing a screened 5d-shell, in place of Zn-diamagnetic ions, caused an increase in the activation energy, Fermi energy, and Fermi temperature compared to the pure ZnCr2Se4. The boost of thedcmagnetic field induced a shift ofTNtowards lower temperatures and a spin fluctuation peak visible at Hdc= 40 and 50 kOe. The obtained single crystals are thermally stable up to 1100 °C.