Removal of quartz from iron ore was accomplished industrially via an anionic reverse flotation technique. However, in that kind of flotation, the interaction of the flotation reagents with the components of the feed sample makes the flotation a complicated system. Thus, the selection and optimization of regent dosages at various temperatures were performed using a uniform experimental design to estimate the optimum separation efficiency. Besides, the produced data as well as the reagent system were mathematically modeled at different flotation temperatures, and the graphical user interface GUI of MATLAB was conducted. The advantage of this procedure is that the user interface displayed in real-time can be conducted by adjusting the temperature at different values to automatically control the reagent system, besides predicting the concentrate yield, total iron grade, and total iron recovery.