Dynamic oxygen-17 (17O) magnetic resonance imaging (MRI) is an imaging method that enables a direct and non-invasive assessment of cerebral oxygen metabolism and thus potentially the distinction between viable and non-viable tissue employing a three-phase inhalation experiment. The purpose of this investigation was the first application of dynamic17O MRI at 7 Tesla (T) in a patient with stroke. In this proof-of-concept experiment, dynamic17O MRI was applied during17O inhalation in a patient with early subacute stroke. The analysis of the relative17O water (H217O) signal for the affected stroke region compared to the healthy contralateral side revealed no significant difference. However, the technical feasibility of17O MRI has been demonstrated paving the way for future investigations in neurovascular diseases.