The cultivar and fertilization can affect the physicochemical properties of pepper fruit. This study aimed at estimating the content of α-carotene, β-carotene, total carotenoids, and the total sugars of unfertilized pepper and samples treated with natural fertilizers based on texture parameters determined using image analysis. Pearson’s correlation coefficients, scatter plots, regression equations, and coefficients of determination were determined. For red pepper Sprinter F1, the correlation coefficient (R) reached 0.9999 for a texture from color channel B and −0.9999 for a texture from channelYfor the content of α-carotene, −0.9998 (channela) for β-carotene, 0.9999 (channela) and −0.9999 (channelL) for total carotenoids, as well as 0.9998 (channelR) and −0.9998 (channela) for total sugars. The image textures of yellow pepper Devito F1were correlated with the content of total carotenoids and total sugars with the correlation coefficient reaching −0.9993 (channelb) and 0.9999 (channelY), respectively. The coefficient of determination (R2) of up to 0.9999 for α-carotene content and the texture from color channelYfor pepper Sprinter F1and 0.9998 for total sugars and the texture from color channelYfor pepper Devito F1were found. Furthermore, very high coefficients of correlation and determination, as well as successful regression equations regardless of the cultivar were determined.