The prevalence ofCampylobacter spp.in pets is a potential concern for human health. However, little is known about the pet-relatedCampylobacterspp. in China. A total of 325 fecal samples were collected from dogs, cats, and pet foxes.Campylobacterspp. were isolated by culture, and MALDI-TOF MS was used to identify 110Campylobacterspp. isolates in total.C. upsaliensis(30.2%, 98/325),C. helveticus(2.5%, 8/325), andC. jejuni(1.2%, 4/325) were the three found species. In dogs and cats, the prevalence ofCampylobacterspp. was 35.0% and 30.1%, respectively. A panel of 11 antimicrobials was used to evaluate the antimicrobial susceptibility by the agar dilution method. AmongC. upsaliensisisolates, ciprofloxacin had the highest rate of resistance (94.9%), followed by nalidixic acid (77.6%) and streptomycin (60.2%). Multidrug resistance (MDR) was found in 55.1% (54/98) of theC. upsaliensisisolates. Moreover, 100 isolates, including 88C. upsaliensis, 8C. helveticus, and 4C. jejuni, had their whole genomes sequenced. By blasting the sequence against the VFDB database, virulence factors were identified. In total, 100% ofC. upsaliensisisolates carried thecadF, porA, pebA, cdtA, cdtB, andcdtCgenes. TheflaAgene was present in only 13.6% (12/88) of the isolates, while theflaBgene was absent. By analyzing the sequence against the CARD database, we found that 89.8% (79/88) ofC. upsaliensisisolates had antibiotic target alteration in thegyrAgene conferring resistance to fluoroquinolone, 36.4% (32/88) had the aminoglycoside resistance gene, and 19.3% (17/88) had the tetracycline resistance gene. The phylogenetic analysis using the K-mer tree method obtained two major clades among theC. upsaliensisisolates. All eight isolates in subclade 1 possessed thegyrAgene mutation, the aminoglycoside and tetracycline resistance genes, and were phenotypically resistant to six classes of antimicrobials. It has been established that pets are a significant source ofCampylobacterspp. strains and a reservoir for them. This study is the first to have documented the presence ofCampylobacterspp. in pets in Shenzhen, China. In this study,C. upsaliensisof subclade 1 required additional attention due to its broad MDR phenotype and relatively highflaAgene prevalence.