﻿AbstractTheBotryosphaerialesrepresents an ecologically diverse group of fungi, comprising endophytes, saprobes, and plant pathogens. In this study, taxonomic analyses were conducted based on morphological characteristics and phylogenetic analyses of multi-gene sequence data from four loci (ITS, LSU,tef1-α, andtub2). Thirteen isolates obtained from Beijing and Yunnan Province were identified as seven species ofBotryosphaeriales, includingAplosporellajaveedii,Dothiorellaalpina,PhaeobotryonaplosporumandPh.rhois, and three previously undescribed species, namelyAplosporellayanqingensis,Dothiorellabaihuashanensis, andPhaeobotryonplatycladi. Additionally, the new records ofDothiorellaalpinafrom the host speciesPopulusszechuanica,PhaeobotryonaplosporumfromJuglansmandshurica, andPhaeobotryonrhoisfromPopulusalbavar.pyramidalisare included.