The shikimate pathway is a general route for the biosynthesis of aromatic amino acids (AAAs) in many microorganisms. A 3-dehydroquinase, AroQ, controls the third step of the shikimate pathway that catalyzes the formation of 3-dehydroquinate from 3-dehydroshikimate via a trans-dehydration reaction.Ralstonia solanacearumharbors two 3-dehydroquinases, AroQ1 and AroQ2, sharing 52% similarity in amino acids. Here, we demonstrated that two 3-dehydroquinases, AroQ1 and AroQ2, are essential for the shikimate pathway inR. solanacearum. The growth ofR. solanacearumwas completely diminished in a nutriment-limited medium with the deletion of botharoQ1andaroQ2, while substantially impairedin planta. ThearoQ1/2double mutant was able to replicate in plantabut grew slowly, which was ~4 orders of magnitude less than the parent strain to proliferate to the maximum cell densities in tomato xylem vessels. Moreover, thearoQ1/2double mutant failed to cause disease in tomato and tobacco plants, whereas the deletion of eitheraroQ1oraroQ2did not alter the growth ofR. solanacearumor pathogenicity on host plants. Supplementary shikimic acid (SA), an important intermediate of the shikimate pathway, substantially restored the diminished or impaired growth ofaroQ1/2double mutant in a limited medium or inside host plants. The necessity of AroQ1 and AroQ2 on the pathogenicity ofsolanacearumtoward host plants was partially due to insufficient SA inside host plants. Moreover, the deletion of botharoQ1andaroQ2significantly impaired the expression of genes for the type III secretion system (T3SS) bothin vitroandin planta. Its involvement in the T3SS was mediated through the well-characterized PrhA signaling cascade and was independent of growth deficiency under nutrient-limited conditions. Taken together,R. solanacearum3-dehydroquinases play important roles in bacterial growth, the expression of the T3SS, and pathogenicity in host plants. These results could extend our insights into the understanding of the biological function of AroQ and the sophisticated regulation of the T3SS inR. solanacearum.