The entomopathogenic fungusAkanthomyces muscariusis commonly used in agriculture to manage insect pests. Besides its use as a commercially important biological control agent, it also presents a potential model for studying host–pathogen interactions and the evolution of virulence in a laboratory setting. Here, we describe the first high-quality genome sequence forA. muscarius. We used long- and short-read sequencing to assemble a sequence of 36.1 Mb with an N50of 4.9 Mb. Genome annotation predicted 12347 genes, with 96.6 % completeness based on the core Hypocrealen gene set. The high-quality assembly and annotation ofA. muscariuspresented in this study provides an essential tool for future research on this commercially important species.