Simple SummaryAFestuloliumgrass is an intergeneric hybrid of fescue (FestucaL.) and ryegrass (LoliumL.) that combines the nutritional qualities and weather resistance of each. Its high nutritional value and resistance to extreme weather conditions are important considerations in the era of global climate change.Festuloliumhas been used as a forage crop for many years, but little information is available on the levels of biologically active compounds (β-carotene and tocopherols) inFestulolium-based diets and in the meat of animals fed such diets. The present study assessed the contents of β-carotene, retinol and tocopherols and the proximate chemical composition of meat from young rams receiving diets based on silage made from differentFestuloliumcultivars (Becva, Felopa, Paulita), as compared with meat from rams fed a diet based on hybrid ryegrass. The meat of animals fedFestuloliumsilage-based diets contained more tocopherols and retinol than the meat of sheep receiving a hybrid ryegrass-based diet. Meat from rams fed diets based on silage made fromFestuloliumcvs. Felopa and Paulita was characterized by the highest concentrations of tocopherols and retinol. The results of this study indicate thatFestuloliumsilage can be a desirable component of ram diets, but its efficacy may vary across cultivars.AbstractThis study analyzed the chemical composition and the β-carotene, tocopherol and retinol content of meat (musculus longissimus lumborum,musculus gluteus maximus) from young rams fed different silage-based diets. The animals were divided into four groups depending on the type of silage in the feed ration (hybrid ryegrass,Festuloliumcv. Becva,Festuloliumcv. Felopa,Festuloliumcv. Paulita). The concentrations of the examined lipophilic compounds were measured via high-performance liquid chromatography (HPLC). Animals fed a diet based on ryegrass silage had the highest intakes of α-, β-, γ- and δ-tocopherol and total tocopherols (p< 0.01). Muscles of rams fedFestuloliumsilage-based diets contained more tocopherols (p< 0.01) and retinol (p< 0.01 inm. g. maximus;p= 0.053 inm. l. lumborum) than the meat of animals receiving hybrid ryegrass-based diets. The highest amounts of tocopherols (p< 0.01) and retinol (p< 0.01 inm. g. maximus;p= 0.053 inm. l. lumborum) were found in meat from rams fed rations based on silage made fromFestuloliumcvs. Felopa and Paulita. The analysis revealed a positive correlation between the intake of tocopherols and the α-tocopherol content of meat (r = 0.99), as well as between the β-carotene intake and the retinol content of meat (r = 0.92 inm. l. lumborum; r = 0.91 inm. g. maximus).