The Fukushima nuclear accident in March 2011 caused biological impacts on the pale grass blue butterflyZizeeria maha. At least some of the impacts are likely mediated by the host plant, resulting in “field effects”. However, to obtain the whole picture of the impacts, direct exposure effects should also be evaluated. Here, we examined the distribution of experimentally ingested anthropogenic cesium-137 (137Cs) in adult butterfly bodies using imaging plate autoradiography. We showed that137Cs ingested by larvae was incorporated into adult bodies and was biased to females, although the majority of ingested137Cs was excreted in the pupal cuticle and excretory material during eclosion.137Cs accumulation in adult bodies was the highest in the abdomen, followed by the thorax and other organs. These results suggest that137Cs accumulation in reproductive organs may cause adverse transgenerational or maternal effects mediated by reactive oxygen species (ROS) on germ cells.137Cs accumulation was detected in field individuals collected in September 2011 and September 2016 but not in May 2011, which is consistent with the abnormality dynamics known from previous studies. Taken together, these results contribute to an integrative understanding of the multifaceted biological effects of the Fukushima nuclear accident in the field.