AbstractSummaryThe outbreak of Mpox virus (MPXV) infection in May 2022 is declared a global health emergency by WHO. A total of 84 330 cases have been confirmed as of 5 January 2023 and the numbers are on the rise. The MPXV pathophysiology and its underlying mechanisms are unfortunately not yet understood. Likewise, the knowledge of biochemicals and drugs used against MPXV and their downstream effects is sparse. In this work, using Knowledge Graph (KG) representations we have depicted chemical and biological aspects of MPXV. To achieve this, we have collected and rationally assembled several biological study results, assays, drug candidates and pre-clinical evidence to form a dynamic and comprehensive network. The KG is compliant with FAIR annotations allowing seamless transformation and integration to/with other formats and infrastructures.Availability and implementationThe programmatic scripts for Mpox KG are publicly available athttps://github.com/Fraunhofer-ITMP/mpox-kg. It is hosted publicly athttps://doi.org/10.18119/N9SG7D.Supplementary informationSupplementary dataare available atBioinformatics Advancesonline.