An organoid is a three-dimensional (3D)in vitrocell
culture emulating human organs. We applied 3D dynamic optical
coherence tomography (DOCT) to visualize the intratissue and
intracellular activities of human induced pluripotent stem cells
(hiPSCs)-derived alveolar organoids in normal and fibrosis models. 3D
DOCT data were acquired with an 840-nm spectral domain optical
coherence tomography with axial and lateral resolutions of 3.8
µm (in tissue) and 4.9 µm, respectively. The DOCT images
were obtained by the logarithmic-intensity-variance (LIV) algorithm,
which is sensitive to the signal fluctuation magnitude. The LIV images
revealed cystic structures surrounded by high-LIV borders and
mesh-like structures with low LIV. The former may be alveoli with a
highly dynamics epithelium, while the latter may be fibroblasts. The
LIV images also demonstrated the abnormal repair of the alveolar
epithelium.