As poultry organ meat is widely consumed, especially in low- and middle-income countries, there is reason to investigate it as a source ofSalmonellainfections in humans. Consequently, the aim of this study was to determine the prevalence, serotypes, virulence factors and antimicrobial resistance ofSalmonellaisolated from chicken offal from retail outlets in KwaZulu-Natal, South Africa. Samples (n= 446) were cultured for the detection ofSalmonellausing ISO 6579-1:2017. PresumptiveSalmonellawere confirmed using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.Salmonellaisolates were serotyped using the Kauffmann–White–Le Minor scheme and antimicrobial susceptibility was determined by the Kirby–Bauer disk diffusion technique. A conventional PCR was used for the detection ofSalmonella invA,agfA,lpfAandsivHvirulence genes. Of the 446 offal samples, 13 tested positive forSalmonella(2.91%; CI = 1.6–5). The serovars includedS.Enteritidis (n= 3/13),S. Mbandaka (n= 1/13),S.Infantis (n= 3/13),S. Heidelberg (n= 5/13) andS. Typhimurium (n= 1/13). Antimicrobial resistance against amoxicillin, kanamycin, chloramphenicol and oxytetracycline was found only inS. Typhimurium andS. Mbandaka. All 13Salmonellaisolates harbouredinvA,agfA,lpfAandsivHvirulence genes. The results show lowSalmonellaprevalence from chicken offal. However, most serovars are known zoonotic pathogens, and multi-drug resistance was observed in some isolates. Consequently, chicken offal products need to be treated with caution to avoid zoonoticSalmonellainfections.