To detect lanes at night, each detecting image is the fusion of the multiple images in a video sequence. The valid lane line detection region is identified on region merging. Then, the image preprocessing algorithm based on the Fragi algorithm and Hessian matrix is applied to enhance lanes; to extract the lane line center feature points, the image segmentation algorithm based on Fractional differential is proposed; and according to the possible lane line positions, the algorithm detects the centerline points in four directions. Subsequently, the candidate points are determined, and the recursive Hough transformation is applied to obtain the possible lane lines. Finally, to obtain the final lane lines, we assume that one lane line should have an angle between 25 and 65 degrees, while the other should have an angle between 115 and 155 degrees, if the detected line is not in the regions, the Hough line detection will be continued by increasing the threshold value until the two lane lines are got. By testing more than 500 images and comparing deep learning methods and image segmentation algorithms, the lane detection accuracy by the new algorithm is up to 70%.