The woodySonchusalliance, a spectacular example of adaptive radiation with six genera and approximately 31 species, is found exclusively on three Macaronesian Islands (Madeira, Canaries, and Cape Verdes) in the Atlantic Ocean. Four of theSonchustaxa are restricted to Madeira, including shrubs and small trees at higher elevations (S.fruticosusandS.pinnatus), and caudex perennials in the lower coastal areas (S.ustulatussubsp.maderensisandS.ustulatussubsp.ustulatus). The MadeiranSonchusstemmed from a single colonization event that originated from the Canaries < 3 million years ago. However, the plastome evolution and species relationships remains insufficiently explored. We therefore assembled and characterized the plastomes of fourSonchustaxa from Madeira and conducted a phylogenomic analysis. We found highly conserved plastome sequences among the taxa, further supporting a single and recent origin. We also found highly conserved plastomes among the cosmopolitan weedySonchus, MacaronesianSonchusin the Atlantic, and Juan Fernández IslandsDendroserisin the Pacific. Furthermore, we identified four mutation hotspot regions (trnK-rps16,petN-psbM,ndhF-Ψycf1, andycf1) and simple sequence repeat motifs. This study strongly supports the monophyly of MadeiranSonchus. However, its relationship with the remaining woodySonchusalliance from the Canary Islands requires further investigation.