Biofilms are a significant concern in the food industry. The utilization of plant-derived compounds to inactivate biofilms on food contact surfaces has not been widely reported. Also, the increasing negative perception of consumers against synthetic sanitizers has encouraged the hunt for natural compounds as alternatives. Therefore, in this study we evaluated the antimicrobial activities of ethanol extracts, acetone extracts, and essential oils (EOs) of seven culinary herbs againstSalmonella entericaserotype Typhimurium andListeria innocuausing the broth microdilution assay. Among all tested extracts and EOs, the ethanol extract ofPiper betleL. exhibited the most efficient antimicrobial activities. To evaluate the biofilm inactivation effect,S. Typhimurium andL. innocuabiofilms on pitted and smooth stainless steel (SS) coupons were exposed toP. betleethanol extract (12.5 mg/ml), sodium hypochlorite (NaClO; 200 ppm), hydrogen peroxide (HP; 1100 ppm), and benzalkonium chloride (BKC; 400 ppm) for 15 min. Results showed that, for the untreated controls, higher sessile cell counts were observed on pitted SS versus smooth SS coupons. Overall, biofilm inactivation efficacies of the tested sanitizers followed the trend ofP. betleextract ≥ BKC > NaClO > HP. The surface condition of SS did not affect the biofilm inactivation effect of each tested sanitizer. The contact angle results revealedP. betleethanol extract could increase the surface wettability of SS coupons. This research suggestsP. betleextract might be utilized as an alternative sanitizer in food processing facilities.