Dermacentor reticulatusticks are sporadically removed from human skin and therefore the medical consequences of their feeding are neglected compared toIxodes ricinus.We investigated the prevalence of pathogens inD. reticulatusremoved from human skin and possible clinical manifestations suggestive of tick-borne diseases after a tick bite. A total of 2153 ticks were studied and of these only 34 wereD. reticulatus. The mean prevalence ofRickettsiainD. reticulatuswas 50.0% andR. raoultiiwas identified in 82.4% of infectedD. reticulatusticks. We confirmed the first case ofR. aeschlimanniiinfection inD. reticulatusticks. Among participants bitten byD. reticulatus, 13.3% reported reddening around the tick bite site and flu-like symptoms, including lymphadenopathy and 3.3% reported eschar on the tick site bite. All of the participants with flu-like symptoms after tick removal were bitten by ticks infected withR. raoultii.The results of this study indicate that even thoughD. reticulatusticks bite humans sporadically, pathogenicRickettsiahave a remarkably high prevalence in this tick species.We can expect that the incidence of tick-borne lymphadenopathy might increase with the reported expansion of theD. reticulatusinto new areas and its growing abundance in Central Europe.