Various surface modification techniques have been developed to improve synthetic polymer surfaces’ wetting, adhesion, and printing by adding various functional (polar) groups. UV irradiation has been proposed as a suitable procedure to achieve adequate surface modifications of such polymers, which can be of further use to bond many compounds of interest. The activation of the surface, the favourable wetting properties, and the increased micro tensile strength of the substrate after short-term UV irradiation suggest that such pretreatment can also improve the bonding of the wood-glue system. Thus, this study aims to determine the feasibility of UV irradiation for pretreatment of wood surfaces before gluing and to determine the properties of wooden glued joints prepared in this way. UV irradiation was used to modify variously machined pieces of beech wood (Fagus sylvaticaL.) before gluing. Six sets of samples were prepared for each machining process. Samples prepared in this manner were exposed to irradiation on a UV line. Each radiation level had a certain number of passes through the UV line, the more passes, the stronger the irradiation. Thus, the radiation levels were as follows: 1, 5, 10, 20, and 50 passes. The dose (energy delivered on the wood surface) in one pass was 2.36 J/cm2. A wetting angle test with glue, a compressive shear strength test of lap joints, and designation of main failure patterns were used to evaluate the properties of wooden glued joints. Wetting angle test was performed according to EN 828, while the compressive shear strength test samples were prepared and tested following the ISO 6238 standard. The tests were conducted using a polyvinyl acetate adhesive. The study found that UV irradiation before gluing improved the bonding properties of variously machined wood.