﻿AbstractComparatively few species of the insectivorous genusPinguiculaL. have been recognized in South America so far. In recent years, a number of narrowly endemic taxa from the Andes have been described that simultaneously refined the broad taxonomic concepts of the “historical” species. Here, we describe two striking new species from Southern Ecuador that further condense the circumscription ofPinguiculacalyptrataKunth.Pinguiculajimburensissp. nov.andP.ombrophilasp. nov.are clearly beyond the taxonomic scope of the known species and consequently described as new to science. The deviating morphological features of the two new taxa are described and illustrated and the remaining morphological spectrum ofP.calyptratain Ecuador is outlined. The two new species add to the exceptional biodiversity in the Amotape-Huancabamba Zone and underline its importance as a biodiversity hotspot in urgent need of protection.