Adenosine nucleoside is an important molecule in human
physiology.
The levels of adenosine nucleoside in urine and plasma are directly
or indirectly related to diseases such as neurodegenerative diseases
and cancer. In the present study, adenosine-imprinted and non-imprinted
poly(2-hydroxyethyl methacrylate-methacrylic acid) (poly(HEMA-MAA))
surface plasmon resonance (SPR) nanosensors were prepared for the
determination of adenosine nucleoside. First, MAA/adenosine pre-polymerization
complexes were prepared at different molar ratios using adenosine
as a template molecule and methacrylic acid (MAA) as a monomer, and
SPR nanosensor surfaces were optimized by determining the highest
imprinting factor of the chip surfaces. The surfaces of adenosine-imprinted
and non-imprinted SPR nanosensors were characterized by using atomic
force microscopy, ellipsometry, and contact angle measurements. Kinetic
analyses were made with different concentrations in the range of 0.5–400.0
nM for the detection range with a pH 7.4 phosphate buffer solution.
The limit of detection in adenosine aqueous solutions, artificial
plasma, and artificial urine was determined to be 0.018, 0.015, and
0.013 nM, respectively. In the selectivity analysis of the developed
nanosensors, the selectivity of adenosine SPR nanosensors in solutions
at different concentrations was determined by using guanosine and
cytidine nucleosides. The relative selectivity coefficients of adenosine-imprinted
SPR nanosensors for adenosine/cytidine and adenosine/guanosine are
3.836 and 3.427, respectively. Since adenosine-imprinted SPR nanosensors
are intended to be used in medical analysis and research, adenosine
analysis has also been studied in artificial urine and artificial
plasma samples.