Simple SummaryThe rat lungworm,Angiostrongylus cantonensis, is the world’s leading cause of eosinophilic meningitis in humans. It is an emerging zoonotic parasite endemic to Asia and the Pacific Islands that has spread to all continents except Antarctica. In the Canary Islands, its presence was first detected in 2010 on the island of Tenerife. Numerous studies demonstrated the high capacity ofA. cantonensisto colonize new areas, especially its ability to parasitize a wide range of animals. Due to the similarity of the ecosystems that we can find between the islands that make up the Canary Archipelago and the great diversity of species of both vertebrates and terrestrial gastropods, the objective of this study was to analyze several species as possible hosts ofA. cantonensison other islands in the Canary Islands, with the use of molecular tools. The present study confirmed the presence ofA. cantonensisin two mammalian species,Mus musculusandFelis catus, and in four terrestrial gastropod species,Limacus flavus, Milax gagates, Insulivitrina emmersoni, andInsulivitrina oromii. The presence ofA. cantonensisimplies a possible risk to humans and other animals, which justifies the need for control measures to control the possible risk of infection and, thus, prevent public health and veterinary problems.AbstractAngiostrongylus cantonensisis a metastrongyloid nematode and the etiologic agent of angiostrongyliasis, a disease characterized by eosinophilic meningitis. This emerging zoonotic parasite has undergone great expansion, including in some regions of Europe and America. In the Canary Islands, the parasite was first discovered parasitizingRattus rattuson the island of Tenerife in 2010. To date, the distribution of this parasite in the Canary Islands has been restricted to the northern zone and the main cities of Tenerife. Using molecular tools for the sentinel species present in the Canary Islands, this study confirmed the presence of the nematode on two other islands in the Canary Archipelago: La Gomera and Gran Canaria. Furthermore, this emerging parasite was detected, besides in the common definitive hostR. rattus, in wildMus musculusandFelis catusand in four terrestrial gastropod species,Limacus flavus, Milax gagates, Insulivitrina emmersoni, andInsulivitrina oromii, two of them endemic to La Gomera, for the first time, increasing the number of non-definitive host species. This study reinforces the expansion character ofA. cantonensisand highlights the importance of knowledge about sentinel species for identifying new transmission locations that help prevent and control the transmission of the parasite and, thus, prevent public health problems.