To this day, the quest to find new drugs is still a challenge due to the growing demands of patients suffering from chronic inflammatory diseases and the need for the individualization of therapy. The aim of this research was to synthesize new 1,2,4-triazole derivatives containing propanoic acid moiety and to investigate their anti-inflammatory, antibacterial and anthelmintic activity. Compounds3a–3gwere obtained in reactions of amidrazones1a–1gwith succinic anhydride. Several analyses of proton and carbon nuclear magnetic resonance (1H NMR,13C NMR, respectively), as well as high-resolution mass spectra (HRMS), confirmed the structures of 1,2,4-triazole derivatives3a–3g. Toxicity, antiproliferative activity and influence on cytokine release (TNF-α: Tumor Necrosis Factor-α, IL-6: Interleukin-6, IFN-γ: Interferon-γ, and IL-10: Interleukin-10) of the compounds3a–3gwere evaluated in peripheral blood mononuclear cells culture. Moreover, mitogen-stimulated cell culture was used for biological activity tests. The antimicrobial and anthelmintic activity of derivatives3a–3gwere studied against Gram-positive and Gram-negative bacterial strains andRhabditissp. culture. Despite the lack of toxicity, compounds3a–3gsignificantly reduced the level of TNF-α. Derivatives3a,3cand3ealso decreased the release of IFN-γ. Taking all of the results into consideration, compounds3a,3cand3eshow the most beneficial anti-inflammatory effects.