Safety assessment and functional analysis of probiotic candidates are important for their industrial applications.Lactiplantibacillus plantarumis one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes ofL. plantarumLRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain’s probiotic potential. Phylogenetic analysis ofL. plantarumLRCC5310 and related strains showed that LRCC5310 belonged toL. plantarum. However, comparative analysis revealed genetic differences betweenL. plantarumstrains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed thatL. plantarumLRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that theL. plantarumLRCC5310 genome encodes an almost complete vitamin B6biosynthetic pathway. Among fiveL. plantarumstrains, includingL. plantarumATCC 14917T,L. plantarumLRCC5310 detected the highest concentration of pyridoxal 5’-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated thatL. plantarumLRCC5310 could be used as a functional probiotic for vitamin B6supplementation.