There have been some reports demonstrating the biogenic synthesis of silver nanoparticles (AgNPs) usingCalotropis procera(CP) plant extract; however, detailed in-depth debriefing of the vital synthesis parameter for rapid, facile, efficacious synthesis at varied temperatures with effectual characterization of nanoparticles and biomimetic attribute is lacking. This study presents a comprehensive demarcation of the sustainable fabrication of biogenicC. proceraflower extract capped and stabilized silver nanoparticles (CP-AgNPs) synthesis with thorough phytochemical characterization and potential biological application. The results revealed that the successful synthesis of CP-AgNPs was instantaneous with the maximum intensity of the plasmonic peak ~400 nm, while morphological results revealed the cubic shape of nanoparticles. CP-AgNPs were found to present stable, well-dispersed, uniform, high anionic zeta potential, and crystalline nanoparticles with a crystallite size of ~23.8 nm. The FTIR spectra indicated that CP-AgNPs were properly capped by the bioactive ofC. procera. Moreover, the synthesized CP-AgNPs exhibited hydrogen peroxide scavenging efficacy. In addition, CP-AgNPs showed antibacterial and antifungal activity against pathogenic bacteria. CP-AgNPs displayed significant in vitro antidiabetic and anti-inflammatory activity. An efficient and convenient approach for synthesizing AgNPs usingC. proceraflower has been developed with enhanced biomimetic attributes that may be further utilized for water treatment, biosensors, biomedicine, and in allied science.