Quorum sensing is a communication strategy that bacteria use to collectively alter gene expression in response to cell density. Pathogens use quorum sensing systems to control activities vital to infection, such as the production of virulence factors and biofilm formation. ThePseudomonas virulence factor(pvf) gene cluster encodes a signaling system (Pvf) that is present in over 500 strains of proteobacteria, including strains that infect a variety of plant and human hosts. We have shown that Pvf regulates the production of secreted proteins and small molecules in the insect pathogenPseudomonas entomophilaL48. Here, we identified genes that are likely regulated by Pvf using the model strainP.entomophilaL48 which does not contain other known quorum sensing systems. Pvf regulated genes were identified through comparing the transcriptomes of wildtypeP.entomophilaand apvfdeletion mutant (ΔpvfA-D). We found that deletion ofpvfA-Daffected the expression of approximately 300 genes involved in virulence, the type VI secretion system, siderophore transport, and branched chain amino acid biosynthesis. Additionally, we identified seven putative biosynthetic gene clusters with reduced expression in ΔpvfA-D. Our results indicate that Pvf controls multiple virulence mechanisms inP.entomophilaL48. Characterizing genes regulated by Pvf will aid understanding of host–pathogen interactions and development of anti-virulence strategies againstP.entomophilaand otherpvf-containing strains.