The zinc ferrite nano-particles (ZnFe2O4) modified screen-printed graphite electrode (ZnFe2O4/SPGE) was used for the voltammetric determination of vitamin B6in real samples, using differential pulse voltammetry (DPV). It has been found that the oxidation of vitamin B6at the surface of such an electrode occurs at a potential about 150 mV less positive compared to an unmodified screen-printed graphite electrode. After optimization, a vitamin B6 sensor with a linear range from 0.8 to 585.0 μM and a detection limit of 0.17 μM. The ZnFe2O4/SPGE sensor exhibits good resolution between the voltammetric peaks of vitamin B6and vitamin C, making it suitable for detecting vitamin B6in the presence of vitamin C in real samples.