Fusarium pseudograminearumis one of the major fungal pathogens that causeFusariumcrown rot (FCR) worldwide and can lead to a substantially reduced grain yield and quality. Transcription factors play an important role in regulating growth and pathogenicity in plant pathogens. In this study, we identified a putative Zn(II)2Cys6fungal-type domain-containing transcription factor and named it FpUme18. The expression ofFpUME18was induced during the infection of wheat byF. pseudograminearum. TheΔFpume18deletion mutant showed defects in growth, conidial production, and conidial germination. In the responses to the cell wall, salt and oxidative stresses, theΔFpume18mutant inhibited the rate of mycelial growth at a higher rate compared with the wild type. The staining of conidia and mycelia with lipophilic dye FM4-64 revealed a delay in endocytosis whenFpUME18was deleted.FpUME18also positively regulated the expression of phospholipid-related synthesis genes. The deletion ofFpUME18attenuated the pathogenicity of wheat coleoptiles.FpUME18also participated in the production of the DON toxin by regulating the expression ofTRIgenes. Collectively, FpUme18 is required for vegetative growth, conidiation, stress response, endocytosis, and full virulence inF. pseudograminearum.