﻿AbstractPhyllosticta(Phyllostictaceae,Botryosphaeriales) includes plant pathogens, endophytes and saprobes, occurring on various hosts worldwide. During the present study, isolates associated with leaf spots were obtained from the hostsQuercusalienaandViburnumodoratissimum, and identified based on morphological features and phylogenetic inference from the analyses of five loci (ITS, LSU,tef1,actandgapdh). Results supported the introduction of two novel species, namelyPhyllostictaanhuiensisandP.guangdongensis. Phylogenetically,P.anhuiensisandP.guangdongensisformed two well-separated lineages in theP.concentricaandP.capitalensisspecies complexes, distinguishing from all presently accepted species in this genus by DNA sequence data. Morphologically,P.anhuiensisandP.guangdongensishave the typical structure of the genusPhyllosticta, and differed from their closely related species by the length of the conidial appendage.