AimThe purpose of our study was to review a large cohort of athletes of all levels presenting with groin pain who underwent investigation with MRI and to determine what the commonest patterns of injury were. We aimed to explore whether particular findings were commonly found in association and whether measurable gender differences exist in the incidence of specific injuries.Materials and methodsImaging records were reviewed to identify MRI studies of the pelvis performed for the investigation of groin pain in patients who were active in sports/athletic pursuits. Findings were classified and recorded as follows: injury to the common rectus abdominis/adductor longus origin, injury to the short adductor muscles, pubic bone oedema, pubic symphysis degenerative changes, hip joint injury and ‘other’. The prevalence of specific injuries in female athletes compared to males was analysed using relative risk ratios.ResultsA total of 470 athletes underwent MRI for the investigation of groin pain during the study period. Forty-six were female, and 424 were male. Female athletes were significantly less likely to have rectus abdominis-adductor longus (RR = 0.31,p= .017), short adductor (RR = 0.14,p= .005) or hip (RR = 0.41,p= .003) injuries. Pubic bone degenerative changes were much more common in female athletes (RR = 7.37,p= .002).ConclusionSignificant gender differences exist in the frequency with which specific injuries are observed. Female athletes are also significantly underrepresented; this is likely a multifactorial phenomenon; however, the possibility of unconscious referrer bias must be considered.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11845-022-03126-3.