This study was carried out to select the bacteriocinogenic strains amongEnterococcusstrains isolated from Ukrainian traditional dairy products using a low-cost media for screening, that containing molasses and steep corn liquor. A total of 475Enterococcusspp. strains were screened for antagonistic activity againstPseudomonas aeruginosa,Escherichia coli,Staphylococcus aureus, andListeria monocytogenesindicator strains. The initial screening revealed that 34Enterococcusstrains during growth in low-cost medium containing corn steep liquor, peptone, yeast extract, and sucrose produced metabolites with inhibition activity against at least of the indicator strains used. Enterocin genesentA,entP, andentBwere detected in 5Enterococcusstrains by PCR assay. Genes of enterocins A and P were found inE. faecalis58 andEnterococcussp. 226 strains, enterocins B and P – inEnterococcussp. 423, enterocin A – inE. faecalis888 andE. durans248 strains. Bacteriocin-like inhibitory substances (BLIS) produced by theseEnterococcusstrains were thermostable and sensitive to proteolytic enzymes. To our knowledge, this is the first report on the isolation of enterocin-producing wildEnterococcusstrains from traditional Ukrainian dairy products using a low-cost media for screening bacteriocinogenic strains. StrainsE. faecalis58,Enterococcussp. 423, andEnterococcussp. 226 are promising candidates for practical use as producers of bacteriocins with inhibitory activity againstL. monocytogenesusing molasses and steep corn liquor as cheap sources of carbon and nitrogen, that can significantly reduce the cost of industrial bacteriocin production. Further studies will be required to determine the dynamic of bacteriocin production, its structure, and mechanisms of antibacterial action.