Candidiasis is one of the most frequent infections worldwide. In this study, the antimicrobial properties of six strains belonging to theMetschnikowia pulcherrimaclade were evaluated against twentyCandidaandCandida-relatedFilobasidiella neoformansvar.bacillispora(syn.Cryptococcus neoformans) of different origins, employing the agar cross method. The toxic effect of pulcherrimin, a red metabolite that is responsible for the antimicrobial activities ofMetschnikowiaspp., was evaluated in various experimental models. The results of agar tests showed that the selectedM. pulcherrimastrains inhibited the growth of theCandidaand non-Candidastrains. However, inhibition was dependent on the strain and the environment. The presence of peptone, sodium silicate, and a higher incubation temperature decreased the antifungal action of theM. pulcherrimastrains. Pulcherrimin showed cytotoxic and antiproliferative activity, with oxidative stress in cells leading to apoptosis. More research is needed on the mechanism of action of pulcherrimin on somatic cells.