Chagas disease (CD) is a neglected illness affecting approximately seven million individuals, with vector transmission occurring via triatomine bugs. The Rhodniini tribe comprises 24 species, grouped into theRhodniusandPsammolestesgenera. Given the importance of accurately identifying CD vectors, the taxonomy ofPsammolestesspp. was revisited using morphological and morphometric data. Specimens ofP. tertius,P. coreodes, andP. arthuriwere collected, and the morphological characteristics of the head, thorax, abdomen, and eggs were analyzed. Morphometric studies of eggs were also conducted. Dichotomous keys allowing for the differentiation ofPsammolestesspp. were elaborated based on adult insect and egg morphological characteristics. Through these studies, it was possible to differentiate the threePsammolestesspecies and confirm that this genus should not be classified under theRhodniusgenus, contributing to Rhodniini taxonomy.