IntroductionA safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work.MethodsThis study focused on the changes in biofilm activity and related metabolic pathways ofS. aureustreated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS).ResultsThe findings demonstrated that the LAB-CFS treatment considerably slowedStaphylococcus aureus(S. aureus) growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of theS. aureusbiofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites ofS. aureusbiofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways.DiscussionThese findings show that LAB-CFS has a strong potential to combatS. aureusinfections.