ABSTRACTWe report the high-quality genome sequence ofTricholoma matsutakestrain 2001, which was isolated from a mushroom fruiting body in South Korea. The genome has 80 contigs, a size of 162.6 Mb, and anN50value of 5,103,859 bp and will provide insight into the symbiotic association betweenT. matsutakeandPinus densiflora.