﻿AbstractA new subspecies ofLeptideasinapisfrom Northern Iran, discovered by means of DNA barcoding, is described asLeptideasinapistabarestanassp. nov.The new subspecies is allopatric with respect to other populations ofL.sinapisand is genetically distinct, appearing as a well-supported sister clade to all other populations in COI-based phylogenetic reconstructions. Details on karyotype, genitalia, ecology and behaviour for the new subspecies are given and a biogeographical speciation scenario is proposed.