Background and Aim:The availability of fertility markers is crucial for maintaining, protecting, and improving the genetics of Jawa-Brebes (Jabres) cows. Follicle-stimulating hormone receptor (FSHR) and insulin-like growth factor-1 (IGF-1) play critical roles in female reproductive physiology. The single-nucleotide polymorphisms (SNPs)FSHRG-278AandIGF-1C-512Tcorrelate with cows’ fertility traits. This study aimed to identify these SNPs and their potential associations with fertility parameters in Jabres cows.Materials and Methods:Samples were collected from 45 heads of multiparous Jabres cows aged 3–10 years with body condition scores of 2.5–5.0 on a 5-point scale in Brebes Regency, Java, Indonesia. These cows were assigned to fertile (n = 16) and infertile groups (n = 29). Polymerase chain reaction (PCR) was carried out for DNA amplification ofFSHRG-278AandIGF-1C-512Tfragments. Restriction fragment length polymorphism-PCR with the restriction enzymesFaqIfor the product ofFSHRG-278AandSnaBIfor the product ofIGF-1C-512Twas used to identify SNPs.Results:TheFaqIenzyme cut the 211 bp DNA fragment ofFSHRG-278Ain all samples into two bands of 128 bp and 83 bp (GG genotype). Meanwhile, the genotyping of amplicon products ofIGF-1C-512Tgenerated a single 249 bp fragment (CC genotype) in both groups.Conclusion:The results showed that theFSHR G-278A/FaqIandIGF-1 C-512T/SnaBIloci were monomorphic in Jabres cows. Thus, neitherFSHR G-278A/FaqInorIGF-1 C-512T/SnaBIis a possible genetic marker for fertility in Jabres cows.