ObjectiveThe study evaluates the effectiveness of Angelica sinensis (As) adjunctive to Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in dogs.Material and methodA randomized study design was conducted on the third and fourth premolars of six dogs. A total of twenty-four defects were surgically created. After reflecting a mucoperiosteal flap, twelve defects were filled with As granules mixed with β-TCP (Experimental group) while the other twelve defects were filled with β-TCP only (Control group) and both were covered by collagen membrane. At the fourth and eighth weeks, jaw segments were dissected and processed for immune-histological examination and histomorphometry analysis.ResultsAt four and eight weeks after treatment, experimental group showed a statistically significant increase in the height of newly formed interradicular bone (p= 0.001 andp= 0.0001 respectively), its surface area (p= 0.002 andp= 0.02 respectively), and the thickness of its trabeculae (p= 0.0001 andp= 0.001 respectively), when compared to control group. Moreover. alkaline phosphatase immunoreaction showed higher intensity in the osteoblast cells of experimental group compared to control group.ConclusionAs enhances periodontal regeneration and bone-formation when used in the management of furcation defects.