The realization of isotopically enriched Ti targets for nuclear cross-section measurements requires particular attention, from the starting material preparation up to the deposition technique. In this work, a cryomilling process was developed and optimized, aimed at reducing the size of49,50Ti metal sponge as provided by the supplier (size up to 3 mm), to the optimal size of 10 µm, to fit the High Energy Vibrational Powder Plating technique used for target manufacturing. The optimization of the cryomilling protocol and the HIVIPP deposition usingnatTi material was thus performed. The scarce amount of the enriched material to be treated (about 150 mg), the need to obtain a non-contaminated final powder and a uniform target thickness of about 500 µg/cm2were taken into account. The49,50Ti materials were then processed and 20 targets of each isotope were manufactured. Both powders and the final Ti targets produced were characterized by SEM-EDS analysis. The amount of Ti deposited was measured by weighing, indicating reproducible and homogeneous targets, with an areal density of 468 ± 110 µg/cm2for49Ti (n= 20) and 638 ± 200 µg/cm2for50Ti (n= 20). The uniformity of the deposited layer was also confirmed by the metallurgical interface analysis. The final targets were used for the cross section measurements of the49Ti(p,x)47Sc and50Ti(p,x)47Sc nuclear reaction routes aimed at the production of the theranostic radionuclide47Sc.