﻿AbstractDiglyphusWalker, 1844 (Hymenoptera:Eulophidae) is an economically important genus including species acting as biocontrol agents against agromyzid leafminer pests. A new species ofDiglyphus,DiglyphusdifasciatusLiu, Hansson & Wan,sp. nov., was discovered during the identification of agromyzid leafminers and their associated parasitoid wasps collected from 2016 to 2022 in China, based on morphological characteristics and molecular analyses ofCOI,ITS2and28Sgenes.Diglyphusdifasciatusis similar toD.bimaculatusZhu, LaSalle & Huang, distinguished by two interconnected infuscate vertical bands on the fore wing and the color of the scape. Molecular data supportD.difasciatusandD.bimaculatusas two different species. The mean genetic distances betweenD.difasciatusandD.bimaculatuswere 11.33%, 8.62%, and 0.18%, based on theCOI,ITS2, and28Sgenes, respectively.