SignificanceReal-time monitoring of multiple neurochemicals has been challenging due to the crosstalk between sensors. The proposed system enables multiple measurements through the monolithically integrated microfluidic chip on a neural probe. Also, the proposed real-time bimodal neural probe allows the concurrent observation of electrophysiological signals and different neurochemical concentrations from multiple brain regions of a live animal in real time. In this manuscript, we successfully measured bimodal neural activities in vivoin the medial prefrontal cortex and the medial dorsal thalamus of an intact mouse brain. We expect that the system will contribute to not only investigating the role of neurochemicals in neural circuits related to brain function but also developing the drug for a variety of neurochemical-related brain disorders.