Three species of white-toothed shrews of the order Eulipotyphla are present in central Europe: the bicolored (Crocidura leucodon), greater (Crocidura russula) and lesser (Crocidura suaveolens) white-toothed shrews. Their precise distribution in Germany is ill-defined and little is known about them as reservoirs for zoonotic pathogens (Leptospiraspp.,Coxiella burnetii,Brucellaspp.,Anaplasma phagocytophilum,Babesiaspp.,Neoehrlichia mikurensisandBartonellaspp.). We investigated 372Crociduraspp. from Germany (n = 341), Austria (n = 18), Luxembourg (n = 2) and Slovakia (n = 11). West European hedgehogs (Erinaceus europaeus) were added to compare the presence of pathogens in co-occurring insectivores.Crocidura russulawere distributed mainly in western andC. suaveolensmainly in north-eastern Germany.Crocidura leucodonoccurred in overlapping ranges with the other shrews.Leptospiraspp. DNA was detected in 28/227C. russulaand 2/78C. leucodonsamples. Further characterization revealed thatLeptospira kirschnerihad a sequence type (ST) 100.Neoehrlichia mikurensisDNA was detected in spleen tissue from 2/213C. russulasamples. Hedgehogs carried DNA fromL. kirschneri(ST 100),L. interrogans(ST 24),A. phagocytophilumand twoBartonellaspecies. This study improves the knowledge of the current distribution ofCrocidurashrews and identifiesC. russulaas carrier ofLeptospira kirschneri. However, shrews seem to play little-to-no role in the circulation of the arthropod-borne pathogens investigated.