Freshwater acquisition methods under various environments are required because water scarcity has intensified worldwide. Furthermore, as water is an essential resource for humans, a freshwater acquisition method that can be utilized even under harsh conditions, such as waterless and polluted water environments, is highly required. In this study, a three-dimensional (3D) printing-assisted hierarchically structured surface with dual-wettability (i.e., surface with both hydrophobic and hydrophilic region) for fog harvesting was developed by mimicking the biological features (i.e., cactus spines and elytra of Namib Desert beetles) that have effective characteristics for fog harvesting. The cactus-shaped surface exhibited self-transportation ability of water droplet, derived from the Laplace pressure gradient. Additionally, microgrooved patterns of the cactus spines were implemented using the staircase effect of 3D printing. Moreover, a partial metal deposition method using wax-based masking was introduced to realize the dual wettability of the elytra of the Namib Desert beetle. Consequently, the proposed surface exhibited the best performance (average weight of 7.85 g for 10 min) for fog harvesting, which was enhanced by the synergetic effect between the Laplace pressure gradient and surface energy gradient. These results support a novel freshwater production system that can be utilized even in harsh conditions, such as waterless and polluted water environments.