TheBrassica napus(B. napus)LOR(Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research inArabidopsisshowed thatLORfamily members played important roles inHyaloperonospora parasitica(Hpa) defense. Nevertheless, there is a paucity of research investigating the role of theLORgene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56LORgenes inB. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56BnLORscould be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56BnLORmembers have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated thatBnLORsinvolved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern ofBnLORfamily members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validateBnLORgene expression under temperature, salinity and ABA stress, revealing that mostBnLORsshowed inducibility. This study enhanced our comprehension of theB. napus LORgene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.