Although several tissues and chemokines orchestrate coronary formation, the guidance cues for coronary growth remain unclear. Here, we profile the juvenile zebrafish epicardium during coronary vascularization and identifyhapln1a+cells enriched with vascular-regulating genes.hapln1a+cells not only envelop vessels but also form linear structures ahead of coronary sprouts. Live-imaging demonstrates that coronary growth occurs along these pre-formed structures, with depletion ofhapln1a+cells blocking this growth.hapln1a+cells also pre-lead coronary sprouts during regeneration andhapln1a+cell loss inhibits revascularization. Further, we identifyserpine1expression inhapln1a+cells adjacent to coronary sprouts, andserpine1inhibition blocks vascularization and revascularization. Moreover, we observe thehapln1asubstrate, hyaluronan, forming linear structures along and preceding coronary vessels. Depletion ofhapln1a+cells orserpine1activity inhibition disrupts hyaluronan structure. Our studies reveal thathapln1a+cells andserpine1are required for coronary production by establishing a microenvironment to facilitate guided coronary growth.