﻿AbstractTrichodermaspp. are diverse fungi with wide distribution. In this study, we report on three new species ofTrichoderma, namelyT.nigricans,T.densissimumandT.paradensissimum, collected from soils in China. Their phylogenetic position of these novel species was determined by analyzing the concatenated sequences of the second largest nuclear RNA polymerase subunit encoding gene (rpb2) and the translation elongation factor 1– alpha encoding gene (tef1). The results of the phylogenetic analysis showed that each new species formed a distinct clade:T.nigricansis a new member of theAtrovirideClade, andT.densissimumandT.paradensissimumbelong to theHarzianumClade. A detailed description of the morphology and cultural characteristics of the newly discoveredTrichodermaspecies is provided, and these characteristics were compared with those of closely related species to better understand the taxonomic relationships within theTrichoderma.