Antimicrobial resistance is a major public health concern. The aim of this study was to assess the presence of antibiotic resistance genes, previously reported inHelicobacter pylori,in gastric samples of 36 pigs, in which DNA ofH. pylori-like organisms had been detected. Based on PCR and sequencing analysis, two samples were positive for the16S rRNAmutation gene, conferring tetracycline resistance, and one sample was positive for thefrxAgene with a single nucleotide polymorphism, conferring metronidazole resistance. All three amplicons showed the highest homology withH. pylori-associated antibiotic resistance gene sequences. These findings indicate that acquired antimicrobial resistance may occur inH. pylori-like organisms associated with pigs.