ObjectiveThis study aimed to determine the effects of soybean (Glycine
max) administration on ZP2 expression in female mice.MethodsThis research usedMus musculus, six-week-old female SWISS
strain mice divided into three groups (group without soybean administration
and groups with mixed feed with soybeans and pelleted 50:50 and 25:75).
Soybean feed for mice was 360 grams per kilogram of mouse body weight for 2
weeks. The percentage of follicles was measured and analyzed using
Hematoxylin-Eosin staining, and the expression of ZP2 was analyzed using
immunohistochemistry. We assessed the data using one-way ANOVA and paired
t-test using the SPSS 17.ResultsSome of the follicles in the ovaries do not develop until their final stage
of follicle maturation. The administration of soybean before and after
treatment in all groups was not significantly different, but the numbers of
atretic follicles in groups 1 and 2 were significantly different. Soybean
administration at a ratio of 50:50 has the effect of increasing the
percentage of the ZP2 expression in tertiary follicles
(p=0.001), whereas soybean administration at a ratio of
25:75 was not able to maintain or increase the formation of ZP2 in tertiary
follicles (p=0.77).ConclusionSoybean administration with a ratio of 50:50 significantly increased the
percentage of the ZP2 expression in tertiary follicles.