Ride comfort is one of the key issues in passenger transport. Its level depends on many factors related to both environmental factors and individual human characteristics. Ensuring good travel conditions translates into higher quality transport services. This article presents a literature review, which shows that ride comfort is most often considered in the context of the impact of mechanical vibrations on the human body, while other factors are usually neglected. The aim of this study was to conduct experimental studies that take into account more than one type of ride comfort. These studies concerned metro cars in the Warsaw metro system. Three types of comfort were evaluated: vibrational, thermal, and visual, based on vibration acceleration measurements, air temperature, relative air humidity, and illuminance. The ride comfort in the vehicle bodies’ front, middle, and rear parts was tested under typical running conditions. The criteria for assessing the effect of individual physical factors on ride comfort were selected based on applicable European and international standards. The test results indicate good thermal and light environment conditions in every measuring point. The slight decrease in passenger comfort is undoubtedly due to the effects of vibrations occurring while mid journey. In tested metro cars, horizontal components have a more significant impact on reducing vibration comfort than other components.