Immune cells and stromal cells regulate wound healing and regeneration through complex activation patterns with spatiotemporal variation. The scarless regeneration of Spiny mice (Acomysspecies) is no exception; differential activation of immune and stromal cell populations seems to play a role in its remarkable regenerative capacity. To elucidate the role and interplay of Acomys immune cells in mammalian regeneration, we sought to createAcomys-Muschimeras by transplanting bone marrow (BM) fromAcomysinto NOD Scid Gamma (NSG), a severely immunodeficient mouse line often used in creating humanized mice. Here, we report thatAcomysBM cells fail to reconstitute and differentiate when transferred to irradiated NSG adults and neonates. In addition, we did not detect the presence of donor cells nor observe the onset of Graft versus Host Disease (GvHD)-like pathology, even after transplantingAcomyssplenocytes inAcomys-Muschimeras suggesting early graft failure. Overall, these results demonstrate the adoptive transfer ofAcomysBM alone is not sufficient to establishAcomyshematopoietic system in NSG mouse.