TIFYs are plant-specific transcription factors that contain the TIFY structural domain and play an important role in plant leaf growth and development. However, the role played by TIFY inE. ferox(Euryale feroxSalisb.) leaf development has not been investigated. In this study, 23TIFYgenes were identified inE. ferox. Phylogenetic analyses of theTIFYgenes showed clustering into three groups (JAZ, ZIM, and PPD). The TIFY domain was shown to be conserved. JAZ was mainly expanded via wholegenome triplication (WGT) inE. ferox.Based on analyses of theTIFYgenes in nine species, we found that JAZ has a closer relationship with PPD, in addition to appearing the most recently and expanding most rapidly, leading to the rapid expansion of TIFYs inNymphaeaceae. In addition, their different evolution types were discovered. Different gene expressions showed the distinct and corresponsive expression patterns of theEfTIFYsin different stages of tissue and leaf development. Finally, The qPCR analysis revealed that the expression ofEfTIFY7.2andEfTIFY10.1showed an upward trend and high expression throughout leaf development. Further co-expression analysis indicated thatEfTIFY7.2might be more important for the development ofE. feroxleaves. This information will be valuable when exploring the molecular mechanisms ofEfTIFYs in plants.