AbstractObjectiveTo assess whether knowledge of Tuskegee, the U.S. Immigration and Customs Enforcement (ICE) agency's detainment of children, and satisfaction with the George Floyd death investigation were associated with trust in actors involved in the development and distribution of coronavirus vaccines.Data Sources and Study SettingNational survey with a convenience sample of Black (n= 1019) and Hispanic (n= 994) adults between July 1 and 26, 2021.Study DesignObservational study using stratified adjusted logistic regression models to measure the association between ratings of the trustworthiness of actors involved in the development and distribution of coronavirus vaccines.Principal FindingsAmong Black respondents, lower satisfaction with the George Floyd death investigation was associated with lower trustworthiness ratings of pharmaceutical companies (ME: −0.09; CI: −0.15, 0.02), the FDA (ME: −0.07; CI: −0.14, −0.00), the Trump Administration (ME: −0.09; CI: −0.16, −0.02), the Biden Administration (ME: −0.07, CI: −0.10, 0.04), and elected officials (ME: −0.10, CI: −0.18, −0.03). Among Hispanic respondents, lower satisfaction was associated with lower trustworthiness ratings of the Trump Administration (ME: −0.14, CI: −0.22, −0.06) and elected officials (ME: −0.11; CI: −0.19, −0.02). Greater knowledge of ICE's detainment of children and families among Hispanic respondents was associated with lower trustworthiness ratings of state elected officials (ME: −0.09, CI: −0.16, 0.01). Greater knowledge of the US Public Health Service Study of Syphilis in Tuskegee was associated with higher trustworthiness ratings of their usual source of care (ME: 0.09; CI: 0.28, 0.15) among Black respondents (ME: 0.09; CI: 0.01, 0.16).ConclusionsAmong Black respondents, lower satisfaction with the George Floyd death investigation was associated with lowered levels of trust in pharmaceutical companies, some government officials, and administrators; it was not associated with the erosion of trust in direct sources of health care delivery, information, or regulation. Among Hispanic respondents, greater knowledge of the ICE detainments was associated with lower trustworthiness ratings of elected state officials. Paradoxically, higher knowledge of the Study of Syphilis in Tuskegee was associated with higher trustworthiness ratings in usual sources of care.