﻿AbstractThree new segmented trapdoor spider species belonging to the familyHeptathelidaeKishida, 1923, i.e.,Luthelaasukasp. nov.(♂♀, Sichuan),L.beijingsp. nov.(♂♀, Beijing), andL.kagamisp. nov.(♂♀, Sichuan), are described from China. Their phylogenetic position and relationships withinHeptathelidaeare tested and assessed using a combination available COI data downloaded from GenBank with new DNA sequences obtained in this study. The results show that the new species form a clade with eight known and one undescribed species ofLuthela. High-definition illustrations of the male palps and female genitalia, diagnoses, and DNA barcodes are provided for these three new species, and their distributions are mapped.