The increased consumption
of blue-green algae (BGA)-based
dietary
supplements has raised concern about their food safety, especially
about cyanotoxin presence. The hyphenation of liquid chromatography
with ion mobility mass spectrometry represents a relevant tool to
screen several compounds in a large variety of food matrices. In this
work, ultrahigh-performance liquid chromatography coupled to traveling
wave ion mobility spectrometry/quadrupole time-of-flight mass spectrometry
(UHPLC-TWIMS-QTOF) was employed to establish the first comprehensive
TWIMS-derived collision cross section database (TWCCSN2) for phycotoxins. The database included 20 cyanotoxins and
1 marine toxin. Accuratem/z, retention
times, andTWCCSN2values were obtained for
81 adducts in positive and negative electrospray (ESI+/ESI–) modes. Reproducibility and robustness of theTWCCSN2measurements were determined to be independent
of the matrix. A screening was carried out on 19 commercial BGA dietary
supplements of different composition. Cyanotoxins were confidently
identified in five samples based on retention time,m/z, andTWCCSN2.