The purpose of this study was to estimate the workloads accumulated by collegiate female soccer players during a competitive season and to compare the workloads of starters and substitutes. Data from 19 college soccer players (height: 1.58 ± 0.06 m; body mass: 61.57 ± 6.88 kg) were extracted from global positioning system (GPS)/heart rate (HR) monitoring sensors to quantify workload throughout the 2019 competitive season. Total distance, distance covered in four speed zones, accelerations, and time spent in five HR zones were examined as accumulated values for training sessions, matches, and the entire season. Repeated-measures ANOVA and Student’sttests were used to determine the level of differences between starter and substitute workloads. Seasonal accumulated total distance (p< 0.001), sprints (≥19.00 km/h;p< 0.001), and high-speed distance (≥15.00 km/h;p= 0.005) were significantly greater for starters than substitutes. Accumulated training load (p= 0.08) and training load per minute played in matches (p= 0.08) did not differ between starters and substitutes. Substitutes had similar accumulated workload profiles during training sessions but differed in matches from starters. Coaches and practitioners should pursue strategies to monitor the differences in workload between starters and substitutes.