Pectobacteriumspp. infect many horticultural crops worldwide and lead to serious crop losses. Zinc-uptake-regulator (Zur) proteins are present widely in prokaryotes and play an important role in pathogenicity. To uncover the role of Zur inP.odoriferum, we constructed mutant (ΔZur) and overexpression [Po (Zur)] strains of aZur, and a virulence assay showed that the Po (Zur) was of significantly lower virulence, while theΔZurdisplayed significantly increased virulence on Chinese cabbage compared to their respective control strains, wild-typeP.odoriferum(Po WT) andP.odoriferumharboring an empty vector (Po (EV)) (p< 0.05). The growth curves of theΔZurand Po (Zur) showed no obvious differences from those of the control strains. Comparative transcriptome analysis showed thatZuroverexpression inP.odoriferuminduced differentially expressed genes (DEGs) related to flagellum and cell motility, while mutatingZurresulted in DEGs mainly corresponding to divalent-metal-ion transport and membrane transport. Phenotypic experiments on the Po (Zur) showed that flagellum numbers and cell motility were reduced in comparison with the control, while those of theΔZurdid not change. Collectively, these results show that the Zur negatively regulates the virulence ofP.odoriferumand might function via a dual mechanism dependent on dose.