A comparative assessment of AgNPs synthesized through three different routes viz.clove bud extract mediated AgNPs, sodium borohydride AgNPs and Glutathione (GSH) capped AgNPs for antioxidant and mosquito larvicidal activities was the major focus of the present study. The nanoparticles were characterized using UV–VIS spectrophotometry, dynamic light scattering (DLS), X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Characterization studies revealed the synthesis of stable, crystalline AgNPs measuring 28 nm, 7 nm and 36 nm for green, chemical and GSH-capped AgNPs respectively. FTIR analysis exhibited the surface functional moieties that were responsible for reduction, capping and stabilizing AgNPs. Antioxidant activity was found to be 74.11%, 46.62% and 58.78% for clove, borohydride and GSH-capped AgNPs respectively. Mosquito larvicidal bioactivity of AgNPs againstAedes aegyptiIIIrd instar larvae depicted clove AgNPs being most effective (LC50—4.9 ppm, LC90—30.2 ppm) followed by GSH-capped (LC50—20.13 ppm, LC90—46.63 ppm) and borohydride AgNPs (LC50—13.43 ppm, LC90—160.19 ppm) after 24 h. Toxicity screening against aquatic modelDaphnia magnarevealed Clove mediated and GSH-capped AgNPs to be safer as compared to the borohydride AgNPs. It may be envisaged that green and capped AgNPs may be further explored for diverse biomedical and therapeutic applications.