AbstractRemorins, plant-specific proteins, have a significant role in conferring on plants the ability to adapt to adverse environments. However, the precise function of remorins in resistance to biological stress remains largely unknown. EighteenCaREMgenes were identified in pepper genome sequences based on the C-terminal conserved domain that is specific to remorin proteins in this research. Phylogenetic relations, chromosomal localization, motif, gene structures, and promoter regions of these remorins were analyzed and a remorin gene,CaREM1.4, was cloned for further study. The transcription ofCaREM1.4in pepper was induced by infection withRalstonia solanacearum. Knocking downCaREM1.4in pepper using virus-induced gene silencing (VIGS) technologies reduced the resistance of pepper plants toR. solanacearumand downregulated the expression of immunity-associated genes. Conversely, transient overexpression ofCaREM1.4in pepper andNicotiana benthamianaplants triggered hypersensitive response-mediated cell death and upregulated expression of defense-related genes. In addition, CaRIN4-12, which interacted with CaREM1.4 at the plasma membrane and cell nucleus, was knocked down with VIGS, decreasing the susceptibility ofCapsicum annuumtoR. solanacearum. Furthermore, CaREM1.4 reduced ROS production by interacting with CaRIN4-12 upon co-injection in pepper. Taken together, our findings suggest that CaREM1.4 may function as a positive regulator of the hypersensitive response, and it interacts with CaRIN4-12, which negatively regulates plant immune responses of pepper toR. solanacearum.Our study provides new evidence for comprehending the molecular regulatory network of plant cell death.