Taking non-contact temperature measurements in narrow areas or confined spaces of non-uniform surfaces requires high spatial resolution and independence of emissivity uncertainties that conventional cameras can hardly provide. Two-color optical fiber (OF) pyrometers based on standard single-mode (SMF) and multi-mode optical fibers (MMF) with a small core diameter and low numerical aperture in combination with associated commercially available components can provide a spatial resolution in the micrometer range, independent of the material’s emissivity. Our experiment involved using a patterned microheater to generate temperatures of approximately 340 °C on objects with a diameter of 0.25 mm. We measured these temperatures using two-color optical fiber pyrometers at a 1 kHz sampling rate, which were linearized in the range of 250 to 500 °C. We compared the results with those obtained using an industrial infrared camera. The tests show the potential of our technique for quickly measuring temperature gradients in small areas, independent of emissivity, such as in microthermography. We also report simulations and experiments, showing that the optical power gathered via each channel of the SMF and MMF pyrometers from hot objects of 250 µm is independent of distance until the OF light spot becomes larger than the diameter of the object at 0.9 mm and 0.4 mm, respectively.