Telomere length (TL) is a well-known marker of age-related diseases. Oxidative stress and inflammation increase the rate of telomere shortening, triggering cellular senescence. Although lipoproteins could have anti-inflammatory and proinflammatory functional properties, the relationship between lipoprotein particles with TL and telomerase activity-related genes has not been investigated much. In this study, we assessed the associations of lipoprotein subfractions with telomere length,TERT, andWRAP53expression in a total of 54 pre-diabetic subjects from the EPIRDEM study. We regressed TL,TERT, andWRAP53on 12 lipoprotein subclasses, employing a Gaussian linear regression method with Lasso penalty to determine a lipoprotein profile associated with telomere-related parameters. The covariates included age, sex, body mass index (BMI), dyslipidemia, statin consumption, and physical activity leisure time. We identified a lipoprotein profile composed of four lipoprotein subfractions associated with TL (Pearson r = 0.347,p-value = 0.010), two lipoprotein subfractions associated withTERTexpression (Pearson r = 0.316,p-value = 0.020), and five lipoprotein subfractions associated withWRAP53expression (Pearson r = 0.379,p-value =0.005). After adjusting for known confounding factors, most lipoprotein profiles maintained the association with TL,TERT, andWRAP53. Overall, medium and small-sized HDL particles were associated with shorter telomeres and lower expression ofTERTandWRAP53. Large HDL particles were associated with longer telomere and lower expression ofWRAP53, but not withTERT. Our results suggest that the lipoprotein profiles are associated with telomere length,TERT, andWRAP53expression and should be considered when assessing the risk of chronic diseases.