Candida albicansis a highly drug-resistant fungus for which new treatments are urgently needed due to the lack of clinically effective options. In this study, we evaluated the antifungal activity and mechanism of plasma-activated Ezhangfeng Cuji (PAEC) againstCandida albicansand compared it with physiological saline (PS), plasma-activated physiological saline (PAPS) and Ezhangfeng Cuji (EC). After dielectric barrier discharge (DBD) plasma treatment with EC for 20 min followed by a 10 min immersion ofCandida albicans, the fungus was reduced by approximately 3 orders of magnitude. High performance liquid chromatography (HPLC) results showed an increase of 41.18% and 129.88% in the concentration of oxymatrine and rhein, respectively, after plasma-treated EC. The concentrations of reactive species (RS), such as H2O2,\documentclass[12pt]{minimal}
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				\begin{document}$${\text{NO}}_{3}^{ - }$$\end{document}NO3-, and O3, were found to be higher and the pH value was getting lower in PS after plasma treatment. Detailed analysis of intracellular material leakage, reactive oxygen species (ROS), apoptosis forCandida albicansand observation by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) demonstrated that PAPS, EC and PAEC disrupt the morphological structure ofCandida albicansto varying degrees.Additionally, specific analyses onCandida albicansvirulence factors, such as adhesion to tissue surfaces, cell surface hydrophobicity (CSH), the transition of yeast-phase cells to mycelium-phase cells, and the secretion of hydrolytic enzymes forCandida albicanswere conducted and found to be inhibited after PAPS/EC/PAEC treatment. In our investigation, the inhibitory effects onCandida albicanswere ranked from strong to weak as follows: PAEC, EC, PAPS, and PS.