BackgroundAlthough evolutionary theory is foundational and integrative in modern biology, there remains widespread lack of acceptance among U.S. residents. An interdisciplinary approach to teaching evolutionary theory at the undergraduate level has many advantages, such as giving students a context for learning about evolution and application of evolutionary theory to other academic disciplines and everyday life. While there are foundational examples of interdisciplinary approaches to teaching evolutionary theory, there are few examples of courses with application of evolutionary theory to issues of sustainability, such as conservation or global climate change. We build on the practical and theoretical work of others to create an interdisciplinary course on evolutionary theory for non-science majors, with ties to sustainability. Our course is taught in three modules, with extensive readings and hands-on lab activities. The first module is focused on honey bee biology, with hands-on beekeeping experiences; the second module on native plants and community education on sustainability; and the third module on the evolution of the subjective human experience of free will.ResultsWe found that students in our course experienced an increased acceptance of evolutionary theory. We found that students also met the course leaning objectives, of basic knowledge of evolutionary theory and application of evolutionary theory to other disciplines, assessed through group and individual major assignments. We also found that students had an expanded perspective on interdisciplinary application of evolutionary theory, assessed through closed-ended survey questions and analysis of open-ended writing.ConclusionsStudents in our course experienced an increase of acceptance of evolutionary theory and an expanded perspective on interdisciplinary application of evolutionary theory, despite the fact that many students were not science majors.Supplementary InformationThe online version contains supplementary material available at 10.1186/s12052-023-00188-4.