This study explored the prevalence of multi-drug resistance and virulence factors of enterococcal isolates obtained from various clinical specimens (n= 1575) including urine, blood, pus, tissue, catheter, vaginal wash, semen, and endotracheal secretions. Out of 862 enterococcal isolates, 388 (45%), 246 (29%), 120 (14%), and 108 (13%) were identified asEnterococcus faecalis,Enterococcus faecium,Enterococcus durans, andEnterococcus hirae, respectively, using standard morphological and biochemical methods. The antibiotic resistance profile of all these enterococcal isolates was checked using the disc diffusion technique. High-level resistance was observed for benzylpenicillin (70%) and vancomycin (43%) amongE. faecalisandE. faeciumisolates, respectively. This study also revealed the prevalence of ‘multi-drug resistance (resistant to 3 antibiotic groups)’ among the vancomycin-resistant enterococcal strains, and this was about 11% (n= 91). The virulence determinants associated with vancomycin resistance (VR) were determined phenotypically and genotypically. About 70 and 39% ofE. faecalisandE. faeciumisolates showed to be positive for all four virulence factors (gelatinase, protease, hemolysin, and biofilm). Among the several virulence genes,gelEwas the most common virulence gene with a prevalence rate of 76 and 69% amongE. faecalisandE. faeciumisolates, respectively. More than 50% of VRE isolates harbored other virulence genes, suchesp,asa,ace, andcylA. Similarly, the majority of the VR enterococcal isolates (n= 88/91) harboredvanAgene and none of them harboredvanBgene. These results disclose the importance of VRE. faecalisandE. faeciumand the associated virulence factors involved in the persistence of infections in clinical settings.