A novel family of julolidine-containing fluorescent rhodols
equipped
with a wide variety of substituents was synthesized in a versatile
two-step process. The prepared compounds were fully characterized
and exhibited excellent fluorescence properties for microscopy imaging.
The best candidate was conjugated to the therapeutic antibody trastuzumab
through a copper-free strain-promoted azide-alkyne click reaction.
The rhodol-labeled antibody was successfully applied forin
vitroconfocal and two-photon microscopy imaging of Her2+
cells.