ABSTRACTWe report the genome sequence of a lytic phage named UF_RH6, which infectsPseudomonas aeruginosa. This phage was isolated from a respiratory secretion sample from a patient with pulmonaryP. aeruginosa. UF_RH6 belongs to the familyCaudoviricetesand the genusSamunavirus. Its genome is 94,715 bp in length and encodes 130 proteins.