The lime plant is a horticultural plant that grows in tropical regions. One of the cultivation maintenances to increase the production of lime fruits is pruning. However, the pruning technique of lime requires high production costs. In addition, phytohormones such as ethylene and abscisic acid have regulation to help drop leaves and branches. The study aimed to identify genes in lime involved in the self-pruning process during ethephon and abscisic acid treatments. Total RNA was extracted and subjected to long-read sequencing using a PCR-cDNA sequencing kit, Oxford Nanopore Technologies. The transcripts were produced 5,914 using the RATTLE program and ranged from 201 – 8,156 bp, and N50 was 1,292 bp. The RNA-seq dataset is available as a raw sequence read that scientists can further process and analyze, and this data can be helpful for lime breeding programs that can shed branches and leaves.