AbstractThe mitochondrial genome ofCamellia nitidissimawas sequenced by Illumina and Pacbio sequencing. The results of sequences showed that a total length was 949,915 bp, and the GC content was 45.7% in assembled mitochondrial genome ofC. nitidissima.71 unigenes had been found, including 36 coding proteins and 35 non-coding proteins. Subsequently, the phylogenetic tree was built on 24 plants with the maximum-likelihood method, which had high bootstrap value and fited to the angiosperm phylogeny group classification (APG IV). The study’s findings unravel the taxonomic status ofC. nitidissimaand benefit the evolution study.