The development of drug-resistance in the opportunistic pathogenEscherichia colihas become a global public health concern. Due to the share of similar flora between pets and their owners, the detection of pet-origin antibiotic-resistantE. coliis necessary. This study aimed to detect the prevalence of feline-origin ESBLE. coliin China and to explore the resistance elimination effect of garlic oil to cefquinome on ESBLE. coli. Cat fecal samples were collected from animal hospitals. TheE. coliisolates were separated and purified by indicator media and polymerase chain reaction (PCR). ESBL genes were detected by PCR and Sanger sequencing. The MICs were determined. The synergistic effect of garlic oil and cefquinome against ESBLE. coliwas investigated by checkerboard assays, time-kill and growth curves, drug-resistance curves, PI and NPN staining, and a scanning electronic microscope. A total of 80E. colistrains were isolated from 101 fecal samples. The rate of ESBLE. coliwas 52.5% (42/80). The prevailing ESBL genotypes in China wereCTX-M-1,CTX-M-14, andTEM-116. In ESBLE. coli, garlic oil increased the susceptibility to cefquinome with FICIs from 0.2 to 0.7 and enhanced the killing effect of cefquinome with membrane destruction. Resistance to cefquinome decreased with treatment of garlic oil after 15 generations. Our study indicates that ESBLE. colihas been detected in cats kept as pets. The sensitivity of ESBLE. colito cefquinome was enhanced by garlic oil, indicating that garlic oil may be a potential antibiotic enhancer.