Fungi are a large and diverse group of microorganisms, and although the estimated number of species ranges between 2 and 11 million, only around 150,000 species have been described thus far. The investigation of plant-associated fungi is beneficial for estimating global fungal diversity, for ecosystem conservation, and for the continued development of industry and agriculture. Mango, one of the world’s five most economically important fruit crops, is grown in over 100 countries and has been demonstrated to have a great economical value. During surveys of mango-associated saprobic fungi in Yunnan (China), we discovered three new species (Acremoniisimulans hongheensis,Chaenothecopsis hongheensisandHilberina hongheensis) and five new records. The phylogenetic analyses of multi-gene sequences (LSU, SSU, ITS,rpb2,tef1-αandtub2) coupled with morphological examinations were used to identify all the taxa.