AbstractBacterial communities play multiple functional roles in soil that have positive and negative feedbacks on plant health. However, relatively few studies have focused on the ecology of soil bacterial communities in commercial strawberry production systems. The objective of this study was to determine if ecological processes influencing soil bacterial communities are consistent among commercial strawberry production locations and plots within the same geographic region. Soil samples were collected using a spatially explicit design from three plots in two commercial strawberry production locations in the Salinas Valley region of California. Soil carbon, nitrogen, and pH were measured for each of the 72 soil samples and bacterial communities were characterized using 16 S rRNA sequencing. Multivariate analyses showed bacterial community composition was differentiated between the two strawberry production locations. Analyses of communities within plots demonstrated soil pH and nitrogen were significant predictors of bacterial community composition in one of the three sampled plots. Bacterial communities displayed spatial structure in two plots at one location based on a significant increase in community dissimilarity with increasing spatial distance. Null model analyses identified a lack of phylogenetic turnover among bacterial communities in all plots, but a greater frequency of dispersal limitation in the two plots where spatial structure was also observed. Overall, this work suggests that ecological factors influencing soil bacterial communities are not consistent among different strawberry production locations or plots which may impact the ability to predict or manage the effect of soil microbiomes on strawberry health.