ABSTRACTWe characterized the complete genome of the lyticEnterococcus faecalisphage EFKL, which was isolated from a sewage treatment plant in Kuala Lumpur, Malaysia. The phage, which was classified in the genusSaphexavirus, has a 58,343-bp double-stranded DNA genome containing 97 protein-encoding genes and shares 80.60% nucleotide similarity withEnterococcusphage EF653P5 andEnterococcusphage EF653P3.