ObjectiveVoluntary counseling and testing (VCT) is an integral component of HIV prevention and care strategies worldwide. VCT is considered as an effective strategy in risk reduction among sexually active youth. There is a high burden of HIV in Ethiopia despite a considerable scale up of comprehensive HIV interventions. Hence, the aim of this study was to assess the level of knowledge, attitude and practice of VCT uptake towards HIV among youth of rural part of Tepi town, Ethiopia.MethodA cross sectional study was carried out from May to August 2020. Total numbers of 127 youths in the age group of 15–24 years were recruited in a self-administered questionnaire with 100% response rate. Descriptive analysis was referred to describe the data. Binary logistic regression analysis was used to measure the effect of different variables by computing odds ratio. Multivariable analyses were utilized to determine the relative effect of explanatory variables.ResultsThe study showed that out of 127 total youth respondents, 111 (87.4%) of the respondents have good knowledge and 99 (78%) had a positive attitude towards VCT. Whereas, 86 (67.7%) of the respondents are aware of practicing VCT on HIV in the past.ConclusionsThe findings revel that gender and father’s educational status were among the socio-demographic variables that showed statistically significant association with one or more variables affecting youths’ knowledge towards VCT uptake. As such, perceived risk of HIV infection, educational level, gender and marital status were statistically responsible for VCT uptake.