﻿AbstractThe genusDiaporthe(Diaporthaceae,Diaporthales) comprises endophytes, pathogens and saprophytes, inhabiting a wide range of woody hosts and resulting in serious canker disease. To determine the diversity ofDiaporthespecies associated with canker disease of host plants in Beijing, China, a total of 35 representative strains were isolated from 18 host genera. Three novel species (D.changpingensis,D.diospyrinaandD.ulmina) and four known species (D.corylicola,D.donglingensis,D.eresandD.rostrata) were identified, based on morphological comparison and phylogenetic analyses using partialITS,cal,his3,tef1-αandtub2loci. These results provide an understanding of the taxonomy ofDiaporthespecies associated with canker diseases in Beijing, China.