The family of phosphatidylinositol transfer proteins (PITPs) is able to bind specific lipids to carry out various biological functions throughout different stages of plant life. But the function of PITPs in rice plant is unclear. In this study, 30 PITPs were identified from rice genome, which showed differences in physicochemical properties, gene structure, conservation domains, and subcellular localization. The promoter region of theOsPITPsgenes included at least one type of hormone response element, such as methyl jasmonate (Me JA) and salicylic acid (SA). Furthermore, the expression level ofOsML-1,OsSEC14-3,OsSEC14-4,OsSEC14-15, andOsSEC14-19genes were significantly affected by infection of rice blast fungusMagnaporthe oryzae. Based on these findings, it is possible that OsPITPs may be involved in rice innate immunity in response toM. oryzaeinfection through the Me JA and SA pathway.