Freezing stress is one of the main factors limiting the growth and yield of wheat. In this study, we found thatTaMYB4expression was significantly upregulated in the tillering nodes of the strong cold-resistant winter wheat variety Dongnongdongmai1 (Dn1) under freezing stress. Weighted gene co-expression network analysis, qRT-PCR and protein–DNA interaction experiments demonstrated that monodehydroascorbate reductase (TaMDHAR) is a direct target of TaMYB4. The results showed that overexpression ofTaMYB4enhanced the freezing tolerance of transgenicArabidopsis. InTaMYB4overexpression lines (OE-TaMYB4),AtMDHAR2expression was upregulated and ascorbate-glutathione (AsA–GSH) cycle operation was enhanced. In addition, the expression of cold stress marker genes such asAtCBF1,AtCBF2,AtCBF3,AtCOR15A,AtCOR47,AtKIN1andAtRD29Ain OE-TaMYB4lines was significantly upregulated. Therefore, TaMYB4 may increase freezing tolerance as a transcription factor (TF) inArabidopsisthrough the AsA–GSH cycle and DREB/CBF signaling pathway. This study provides a potential gene for molecular breeding against freezing stress.