Key PointsQuestionDo rare disease experts endorse genome sequencing of newborns to screen for treatable genetic diseases, and do they agree on which genes to include?FindingsIn this survey study of 238 rare disease experts, 87.9% agreed that genomic sequencing for monogenic treatable conditions should be available to all newborns. A total of 42 gene-disease pairs were endorsed by more than 80% of the experts.MeaningIn this study, rare disease experts broadly endorsed screening of newborns with genome sequencing, and there was substantial concordance on a limited number of specific gene-disease pairs for prioritization.