Ankle flexibility and isokinetic knee torque/power generating capacity were previously suggested to contribute or to be correlated to the vertical countermovement jump (CMJ) performance. The aim of this study was to investigate the effect of the passive ankle joint dorsi flexion (θPDF) and the knee muscle’s isokinetic torque and power on the CMJ in adolescent female volleyball players. The θPDFat a knee extension angle of 140 degrees were measured for 37 female post-pubertal volleyball players. Then, the players were assigned to either the flexible (n= 10) or inflexible (n= 14) groups according to earlier recommended criteria. Testing included the CMJ with and without an arm swing, and maximal knee extensions and flexions in 3 angular velocities on an isokinetic dynamometer. CMJ height performed with or without an arm swing (r(22)= 0.563,p= 0.040 andr(22)= 0.518,p= 0.009, respectively) and relative power (r(22)= 0.517,p= 0.010 andr(22)= 0.446,p= 0.030, respectively) were positively correlated with the extensors’ torque at 180°/s and were negatively correlated with the flexibility level of the dominant side ankle (r(22)= −0.529,p= 0.008 andr(22)= −0.576,p= 0.030, respectively). A moderate positive correlation was also revealed between the CMJ height with and without an arm swing and the power of the non-dominant knee extensors (r(22)= 0.458,p= 0.024 andr(22)= 0.402,p= 0.049, respectively) and flexors (r(22)= 0.484,p= 0.016 andr(22)= 0.477,p= 0.018, respectively). Results of the 2 × 2 repeated ANOVA measurements revealed that flexible players jumped significantly (p< 0.05) higher during the CMJs, whilst there was a group effect only on the isokinetic knee extensor muscles’ torque. In conclusion, a more flexible ankle joint and a higher isokinetic knee extensor’s torque generating capacity resulted in higher CMJ performance. Therefore, ankle flexibility should be emphasized in training and is suggested to be included in preseason screening tests of youth female volleyball players.