ABSTRACTCandida auris, a multidrug-resistant human fungal pathogen that causes outbreaks of invasive infections, emerged as four distinct geographical clades. Previous studies identified genomic and proteomic differences in nutrient utilization on comparison toCandida albicans, suggesting that certain metabolic features may contribute toC. aurisemergence. Since no high-throughput clade-specific metabolic characterization has been described yet, we performed a phenotypic screening ofC. aurisstrains from all 4 clades on 664 nutrients, 120 chemicals, and 24 stressors. We identified common and clade- or strain-specific responses, including the preferred utilization of various dipeptides as nitrogen source and the inability of the clade II isolate AR 0381 to withstand chemical stress. Further analysis of the metabolic properties ofC. aurisisolates showed robust growth on intermediates of the tricarboxylic acid cycle, such as citrate and succinic and malic acids. However, there was reduced or no growth on pyruvate, lactic acid, or acetate, likely due to the lack of the monocarboxylic acid transporter Jen1, which is conserved in most pathogenicCandidaspecies. Comparison ofC. aurisandC. albicanstranscriptomes of cells grown on alternative carbon sources and dipeptides as a nitrogen source revealed common as well as species-unique responses.C. aurisinduced a significant number of genes with no ortholog inC. albicans, e.g., genes similar to the nicotinic acid transporterTNA1(alternative carbon sources) and to the oligopeptide transporter (OPT) family (dipeptides). Thus,C. aurispossesses unique metabolic features which could have contributed to its emergence as a pathogen.IMPORTANCEFour main clades of the emerging, multidrug-resistant human pathogenCandida aurishave been identified, and they differ in their susceptibilities to antifungals and disinfectants. Moreover, clade- and strain-specific metabolic differences have been identified, but a comprehensive overview of nutritional characteristics and resistance to various stressors is missing. Here, we performed high-throughput phenotypic characterization ofC. aurison various nutrients, stressors, and chemicals and obtained transcriptomes of cells grown on selected nutrients. The generated data sets identified multiple clade- and strain-specific phenotypes and induction ofC. auris-specific metabolic genes, showing unique metabolic properties. The presented work provides a large amount of information for further investigations that could explain the role of metabolism in emergence and pathogenicity of this multidrug-resistant fungus.