IntroductionBurkholderia thailandensisis a study model forBurkholderia pseudomallei, a highly virulent pathogen, known to be the causative agent of melioidosis and a potential bioterrorism agent. These two bacteria use an (acyl-homoserine lactone) AHL-mediated quorum sensing (QS) system to regulate different behaviors including biofilm formation, secondary metabolite productions, and motility.MethodsUsing an enzyme-based quorum quenching (QQ) strategy, with the lactonaseSsoPox having the best activity onB. thailandensisAHLs, we evaluated the importance of QS inB. thailandensisby combining proteomic and phenotypic analyses.ResultsWe demonstrated that QS disruption largely affects overall bacterial behavior including motility, proteolytic activity, and antimicrobial molecule production. We further showed that QQ treatment drastically decreasesB. thailandensisbactericidal activity against two bacteria (Chromobacterium violaceumandStaphylococcus aureus), while a spectacular increase in antifungal activity was observed against fungi and yeast (Aspergillus niger,Fusarium graminearumandSaccharomyces cerevisiae).DiscussionThis study provides evidence that QS is of prime interest when it comes to understanding the virulence ofBurkholderiaspecies and developing alternative treatments.