Wolbachiahas been shown to induce thelytokous parthenogenesis inTrichogrammaspecies, which have been widely used as biological control agents around the world. Little is known about the changes of bacterial community after restoring arrhenotokous or bisexual reproduction in theT. pretiosum. Here, we investigate the emergence of males ofT. pretiosumthrough curing experiments (antibiotics and high temperature), crossing experiments, and high-throughput 16S ribosomal RNA sequencing (rRNA-seq). The results of curing experiments showed that both antibiotics and high temperatures could cause the thelytokousT. pretiosumto produce male offspring.Wolbachiawas dominant in the thelytokousT. pretiosumbacterial community with 99.01% relative abundance. With the relative abundance ofWolbachiabeing depleted by antibiotics, the diversity and relative content of other endosymbiotic bacteria increased, and the reproductive mode reverted from thelytoky to arrhenotoky inT. pretiosum. Although antibiotics did not eliminateWolbachiainT. pretiosum, sulfadiazine showed an advantage in restoring entirely arrhenotokous and successive bisexual reproduction. This study was the first to demonstrate the bacterial communities in parthenogeneticTrichogrammabefore and after antibiotics or high-temperature treatment. Our findings supported the hypothesis thatWolbachiatiter-dependence drives a reproduction switch inT. pretiosumbetween thelytoky and arrhenotoky.