Anaplasma ovisis a tick-borne obligated intraerythrocytic bacterium that infects domestic sheep, goats, and wild ruminants. Recently, several studies have been carried out using16S rRNAandmsp4genes to identify the genetic diversity ofA. ovis. Instead of these genes, which are known to be highly stable among heterologous strains,Msp1a, which is accepted as a stable molecular marker to classifyA. marginalestrains, was used inA. ovisgenetic diversity studies. The genetic diversity ofA. ovisstrains according to theMsp1agene has not been extensively reported. Therefore, the purpose of this study was to examine the genetic diversity ofA. ovisin goats specifically using analysis of theMsp1agene. Blood samples were taken from the vena jugularis to the EDTA tubes from 293 randomly selected goats (apparently healthy) in the Antalya and Mersin provinces of Mediterranean region of Türkiye. TheMsp1agene ofA. oviswas amplified in all DNA samples through the use of PCR, using a specific set of primers named AoMsp1aF and AoMsp1aR. Among the amplified products, well-defined bands with different band sizes were subjected to sequence analysis. The obtained sequence data were converted into amino acid sequences using an online bioinformatics program and the tandem regions were examined. TheMsp1agene ofA. oviswas amplified in 46.1% (135 out of 293) of the goats. Through tandem analysis, five distinct tandems (Ao8, Ao18, Tr15-16-17) were identified, and it was found that three of these tandems (Tr15-16-17) were previously unknown and were therefore defined as new tandems. The study also involved examination of ticks from goats. It was observed that the goats in the area were infested with several tick species, includingRhipicephalus bursa(888/1091, 81.4%),R. turanicus(96/1091, 8.8%),Dermacentor raskemensis(92/1091, 8.4%),Hyalomma marginatum(9/1091, 0.8%), andR. sanguineuss.l. (6/1091, 0.5%). This study provides important data for understanding the genetic diversity and evolution ofA. ovisbased on tandem repeats in the Msp1a protein.