Students’ use of learning strategies (i.e., what studentsdowhen studying) is linked to their achievement in undergraduate science, technology, engineering, and math (STEM) courses, and several study strategies have been individually associated with course and exam grades in multiple contexts. In this study, we surveyed students in a learner-centered, large-enrollment introductory biology course about their study strategies. We aimed to identify groups of strategies that students often reported together, possibly reflecting broader approaches to studying. Exploratory factor analysis revealed three groups of study strategies frequently co-reported (which we namedhousekeeping strategies,use of course materials, andmetacognitive strategies). These strategy groups map onto a model of learning that associates specific suites of strategies to phases of learning, which correspond to different levels of cognitive and metacognitive engagement. Consistent with previous work, only some study strategies were significantly associated with exam scores: students reporting higheruse of course materialsand ofmetacognitive strategiesearned higher scores on the first course exam. Students who improved on the subsequent course exam reported increasing their use ofhousekeeping strategiesand ofcourse materials. Our findings contribute to a deeper understanding of students’ approaches to studying in introductory college biology and of the relationships between study strategies and achievement. This work may support instructors in adopting intentional classroom practices to foster students’ development as self-regulated learners, able to identify expectations and criteria for success and to implement appropriate and effective study strategies.