IntroductionThe raise of multi-drug resistant bacteria involving carbapenem, colistin, or tigecycline resistance constitutes a threat to public health, which partly results from the transmission of corresponding mobile resistance genes, such asblaKPCandblaNDMfor carbapenem,mcrfor colistin, andtmexCD-toprJgene cluster for tigecycline. Herein, we described the emergence of anAeromonas veroniistrain HD6454 co-harboringblaKPC–2,mcr-3.17, andtmexC3.2-tmexD3.3-toprJ1bgene cluster from hospital sewage.MethodsWhole genome sequencing (WGS) was used to determine the genome sequence of HD6454, and the detailed genomic analysis of genetic elements or regions carrying key antimicrobial resistance genes (ARGs) from HD6454 were performed. Cloning experiment was conducted to confirm the function of key ARGs in mediating antimicrobial resistance. Conjugation experiment was conducted to determine the mobility of the plasmid.ResultsThe results showed that this strain belonged to a novel sequence type (ST) variant ST1016, and carried 18 important ARGs. Among them, theblaKPC–2was carried by non-self-transmissible IncP-6 plasmid, whiletmexC3.2-tmexD3.3-toprJ1bgene cluster andmcr-3.17were carried by integrative and mobilizable element (IME) or IME-related region in chromosome. Themcr-3.17,mcr-3.6, andmcr-3-like3genes were further inferred to originate from IMEs ofAeromonasspecies. Additionally, for the first time, themcr-3.17was confirmed to confer low-level resistance to colistin under inducible expression, whiletmexC3.2-tmexD3.3-toprJ1bgene cluster was confirmed to confer low-level resistance to tigecycline.DiscussionThis is the first report of a strain co-harboringblaKPC–2,mcr-3.17, andtmexC3.2-tmexD3.3-toprJ1bgene cluster. Although the resistance and/or mobility of these ARGs are limited in this strain, the emergence of this multiple important ARGs-carrying strain deserves further attention.