Phytic acid (PA) acts as a storehouse for the majority of the mineral phosphorous (P) in maize; ~80% of the total P stored as phytate P is not available to monogastric animals and thereby causes eutrophication. In addition, phytic acid chelates positively charged minerals making them unavailable in the diet. The mutantlpa1-1allele reduces PA more than the wild-typeLPA1allele. Further, mutant geneopaque2(o2) enhances lysine and tryptophan andcrtRB1enhances provitamin-A (proA) more than wild-typeO2andCRTRB1alleles, respectively. So far, the expression pattern of the mutantlpa1-1allele has not been analysed in maize genotypes rich in lysine, tryptophan and proA. Here, we analysed the expression pattern of wild and mutant alleles ofLPA1,O2andCRTRB1genes in inbreds with (i) mutantlpa1-1,o2andcrtRB1alleles, (ii) wild-typeLPA1allele and mutanto2andcrtRB1alleles and (iii) wild-typeLPA1,O2andCRTRB1alleles at 15, 30 and 45 days after pollination (DAP). The average reduction of PA/total phosphorous (TP) inlpa1-1mutant inbreds was 29.30% over wild-typeLPA1allele. Theo2andcrtRB1-based inbreds possessed ~two-fold higher amounts of lysine and tryptophan, and four-fold higher amounts of proA compared to wild-type alleles. The transcript levels oflpa1-1,o2andcrtRB1genes inlpa1-1-based inbreds were significantly lower than their wild-type versions across kernel development. Thelpa1-1,o2andcrtRB1genes reached their highest peak at 15 DAP. The correlation of transcript levels oflpa1-1was positive for PA/TP (r = 0.980), whereas it was negative with inorganic phosphorous (iP) (r = −0.950). Theo2andcrtRB1transcripts showed negative correlations with lysine (r = −0.887) and tryptophan (r = −0.893), and proA (r = −0.940), respectively. This is the first comprehensive study onlpa1-1expression in the maize inbreds during different kernel development stages. The information generated here offers great potential for comprehending the dynamics of phytic acid regulation in maize.