In this paper, we report developing approaches to tuning the optical behavior of microfluidic devices by infusing smart hybrids of liquid crystal and quantum dots into microchannel confinement. We characterize the optical responses of liquid crystal–quantum dot composites to polarized and UV light in single-phase microflows. In the range of flow velocities up to 10 mm/s, the flow modes of microfluidic devices were found to correlate with the orientation of liquid crystals, dispersion of quantum dots in homogeneous microflows and the resulting luminescence response of these dynamic systems to UV excitation. We developed a Matlab algorithm and script to quantify this correlation by performing an automated analysis of microscopy images. Such systems may have application potential as optically responsive sensing microdevices with integrated smart nanostructural components, parts of lab-on-a-chip logic circuits, or diagnostic tools for biomedical instruments.