﻿AbstractTwo new species of sequestrate fungi are described from south Mexico based on morphological and molecular evidences. Here we describeElaphomycescastilloicharacterized by the yellowish mycelial mat, dull blue gleba and ascospores of 9.7–11.5 µm;Entolomasecotioidesis characterized by the secotioid basidiomata, sulcate, pale cream pileus, and basidiospores of 7–13 × 5–9 µm. Both species grow in montane cloud forest underQuercussp. in the state of Chiapas, Mexico. Descriptions, photographs, and multilocus phylogeny for both species are presented.