ABSTRACTWe report the genome sequence of bacteriophage KpS110, which infectsKlebsiella pneumoniae, a multidrug-resistant encapsulated bacterium that causes severe community-acquired and hospital-acquired infections. The phage genome is 156,801 bp, with 201 open reading frames. KpS110 is most closely related to phages of the familyAckermannviridaeat the genome and proteome levels.