Dendrobium officinaleis one of the most widely used medicinal herbs, especially in Asia. In recent times, the polysaccharide content ofD. officinalehas garnered attention due to the numerous reports of its medicinal properties, such as anticancer, antioxidant, anti-diabetic, hepatoprotective, neuroprotective, and anti-aging activities. However, few reports of its anti-aging potential are available. Due to high demand, the wildD. officinaleis scarce; hence, alternative cultivation methods are being employed. In this study, we used theCaenorhabditis elegansmodel to investigate the anti-aging potential of polysaccharides extracted fromD. officinale(DOP) grown in three different environments; tree (TR), greenhouse (GH), and rock (RK). Our findings showed that at 1000 µg/mL, GH-DOP optimally extended the mean lifespan by 14% and the maximum lifespan by 25% (p< 0.0001). TR-DOP and RK-DOP did not extend their lifespan at any of the concentrations tested. We further showed that 2000 µg/mL TR-DOP, GH-DOP, or RK-DOP all enhanced resistance to H2O2-induced stress (p> 0.05,p< 0.01, andp< 0.01, respectively). In contrast, only RK-DOP exhibited resistance (p< 0.01) to thermal stress. Overall, DOP from the three sources all increased HSP-4::GFP levels, indicating a boost in the ability of the worms to respond to ER-related stress. Similarly, DOP from all three sources decreased α-synuclein aggregation; however, only GH-DOP delayed β-amyloid-induced paralysis (p< 0.0001). Our findings provide useful information on the health benefits of DOP and also provide clues on the best practices for cultivatingD. officinalefor maximum medicinal applications.