The objective of the study was to determine the effects of sex and hunting season on the carcass, meat and fat quality of hunted brown hares (Lepus europaeus). Twenty-two hares of both sexes hunted in winter (December) during two hunting seasons in accordance with the law on hunting in Lithuania were evaluated using reference methods The data were subjected to two-factor analysis of variance in the general linear (GLM) procedure. No significant differences in carcass measurements and muscularity or internal organs between the sexes of brown hares were found; however, the hunting season appeared to affect the size of hares. Thebiceps femoris(BF) thigh muscle of males had lower (p< 0.05) dry matter content and higher (p< 0.05) drip loss compared with females. The hunting season demonstrated an effect (p< 0.001) on protein and hydroxyproline contents in thelongissimus thoracis et lumborum(LTL) and affected dry matter, protein and hydroxyproline contents (p< 0.05,p< 0.001 andp< 0.01, respectively) in BF muscles, and differences in the colour of muscles were also observed. The shear force in the Warner–Bratzler (WB) test was higher (p< 0.001 andp< 0.01, respectively) for LTL and BF muscles during the first hunting season. The hunting season did not affect the total SFA in the intramuscular fat (IMF) of all the tissues, but it affected levels of monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids in the muscles. No differences were found in the total saturated fatty acids (SFA) of both muscles between the sexes, but females demonstrated lower (p< 0.05 andp< 0.01, respectively) and more favourable n-6/n-3 PUFA ratios in the muscles and fat and a lower (p< 0.05) thrombogenic (TI) index in the LTL compared with males.