In this work, we outlined an experimental workflow enabling
the
rapid assessment of primary and secondary nucleation and crystal growth
kinetics. We used small-scale experiments in agitated vials with in
situ imaging for crystal counting and sizing to quantify nucleation
and growth kinetics of α-glycine in aqueous solutions as a function
of supersaturation at isothermal conditions. Seeded experiments were
required to assess crystallization kinetics when primary nucleation
is too slow, especially at lower supersaturations often encountered
in continuous crystallization processes. At higher supersaturations,
we compared results from seeded and unseeded experiments and carefully
analyzed interdependencies of primary and secondary nucleation and
growth kinetics. This approach allows for the rapid estimation of
absolute values of primary and secondary nucleation and growth rates
without relying on any specific assumptions about functional forms
of corresponding rate expressions used for estimation approaches based
on fitting population balance models. Quantitative relationships between
nucleation and growth rates at given conditions provide useful insights
into crystallization behavior and can be explored to rationally manipulate
crystallization conditions for achieving desirable outcomes in batch
or continuous crystallization processes.