Adhesion to living and non-living surfaces is an important virulence trait of the fungal pathogenCandida albicans. Biofilm formation in this organism depends on the expression of a number of cell surface proteins including the hypha-specific protein Als3p. Loss ofALS3impairs biofilm formation and decreases cell-cell adhesion. We wanted to test whether constitutively expressingALS3could compensate for defects in adhesion and biofilm formation observed in mutant strains that lack key transcriptional regulators of biofilm formation Efg1p and Cph1p. We found thatALS3improved adhesion and biofilm formation in theefg1Δ andefg1Δcph1Δ mutant strains, but had less effect on thecph1Δ strain.