BackgroundThe dissemination of carbapenem resistance via carbapenemases, such as the metallo-β-lactamase NDM, amongEnterobacteralesposes a public health threat. The aim of this study was to characterize a plasmid carrying theblaNDM-1gene, which was extracted from a clinicalKlebsiella pneumoniaeuropathogen from an Egyptian patient suffering from a urinary tract infection.Methods and resultsThe recovered plasmid was transformed into competentE. coliDH5α which acquired phenotypic resistance to cefoxitin, ceftazidime, and ampicillin/sulbactam, and intermediate sensitivity to ceftriaxone and imipenem (a carbapenem). Whole plasmid sequencing was performed on the extracted plasmid using the DNBSEQ™ platform. The obtained forward and reverse reads were assembled into contigs using the PRINSEQ and PLACNETw web tools. The obtained contigs were uploaded to PlasmidFinder and ResFinder forin silicoplasmid typing and detection of antimicrobial resistance genes, respectively. The final consensus sequence was obtained using the Staden Package software. The plasmid (pNDMKP37, NCBI accession OK623716.1) was typed as an IncX3 plasmid with a size of 46,160 bp and harbored the antibiotic resistance genesblaNDM-1,bleMBL, andaph(3’)-VI. The plasmid also carried mobile genetic elements involved in the dissemination of antimicrobial resistance including insertion sequences IS30, IS630, and IS26.ConclusionsThis is Egypt’s first report of a transmissible plasmid co-harboringblaNDM-1andaph(3’)-VIgenes. Moreover, the respective plasmid is of great medical concern as it has caused the horizontal transmission of multidrug-resistant phenotypes to the transformant. Therefore, new guidelines should be implemented for the rational use of broad-spectrum antibiotics, particularly carbapenems.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11033-023-08401-9.