AbstractChagas disease is a parasitical disease caused byTrypanosoma cruziwhich affects ∼7 million people worldwide. Per year, ∼10 000 people die from this pathology. Indeed, ∼30% of humans develop severe chronic forms, including cardiac, digestive or neurological disorders, for which there is still no treatment. In order to facilitate research on Chagas disease, a manual curation of all papers corresponding to ‘Chagas disease’ referenced on PubMed has been performed. All deregulated molecules in hosts (all mammals, humans, mice or others) followingT. cruziinfection were retrieved and included in a database, named ChagasDB. A website has been developed to make this database accessible to all. In this article, we detail the construction of this database, its contents and how to use it.Database URLhttps://chagasdb.tagc.univ-amu.fr