We report nine species of the Schistorchiinae Yamaguti, 1942 (Apocreadiidae Skrjabin, 1942) from Indo-Pacific marine fishes. Molecular data (ITS2 and 28S rDNA andcox1 mtDNA) are provided for all species and the genus-level classification of the subfamily is revised. ForSchistorchisLühe, 1906, we report the type-speciesSch. carneusLühe, 1906 andSch. skrjabiniParukhin, 1963. ForSphinteristomumOshmarin, Mamaev & Parukhin, 1961 we report the type-species,Sph. acollumOshmarin, Mamaev & Parukhin, 1961. We report and re-recogniseLobatotremaManter, 1963, for the type and only species,L. aniferumManter, 1963, previously a synonym ofSph. acollum.Lobatotrema aniferumis phylogenetically distant from, but morphologically similar to,Sph. acollumandLobatotremais recognised as a ‘cryptic genus’. We proposeBlendiellan. gen.forB. trigintatestisn. sp.andB. tridecimtestisn. sp.These species are broadly consistent with the present morphological concept ofSchistorchisbut are phylogenetically distant from the type-species; a larger number of testes and some other subtle morphological characters in species ofBlendiellaserve to distinguish the two genera. We report three species ofParaschistorchisBlend, Karar & Dronen, 2017:P. stenosoma(Hanson, 1953) Blend, Karar & Dronen, 2017 (type-species),P. seychellesiensis(Toman, 1989) Blend, Karar & Dronen, 2017, andP. zancli(Hanson, 1953) Blend, Karar & Dronen, 2017.Lobatotrema aniferum, P. stenosoma,andSch. carneuseach have two distinctcox1 populations either over geographical range or in sympatry. Available evidence suggests that most of these species, but not all, are widespread in the tropical Indo-Pacific.