AbstractThe complete mitochondrial genome (mitogenome) of the leafhopperAtkinsoniella nigritaZhang & Kuoh,1993was determined in this study. The entire sequence was 16,011 base pairs (bp) in length. The new mitogenome consists of a typical set of genes: 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and one control region of 1720 bp in length. The base composition of the mitogenome was A = 41.7%, T = 38.2%, C = 10.7%, and G = 9.4%. This is the classical structure for most insect mitogenomes without any gene arrangement observed. Compared with other known mitochondrial genomes of 15Atkinsoniellaspecies, the new mitogenome with three PCGs (ND2,ND5, andND4L) shared the same gene base length, start codon and stop codon, and the shortest12SrRNA (729 bp) and the longesttRNA-Lys(73 bp) within the genusAtkinsoniella. A phylogenetic analysis of 31 Cicadellinae and two Ledrinae concatenated sequences of 13 PCGs of their mitogenomes using Bayesian inference (BI) revealed thatA. nigritabelongs to the genusAtkinsoniellawith strong nodal support (BI posterior probability = 1).