Herein, a novel nanostructure based on cyclic aromatic polyimide with statistical star polymer structure was synthesized via the functionalization of the CuFe2O4MNPs surface. The polymerization process on the functionalized surface of CuFe2O4MNPs was performed with pyromellitic dianhydride and phenylenediamine derivatives. All analytical methods such as Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) analysis, X-ray diffraction (XRD) pattern, energy-dispersive X-ray (EDX), field-emission scanning electron microscope (FE-SEM), vibrating-sample magnetometer (VSM) were performed to characterize the structure of CuFe2O4@SiO2-polymer nanomagnetic. The cytotoxicity of CuFe2O4@SiO2-Polymer was investigated for biomedical application by MTT test. The results proved that this nanocmposite was biocompatible with HEK293T healthy cells. Also, the evaluation antibacterial property of CuFe2O4@SiO2-Polymer showed that its MIC in Gram-negative and Gram-positive bacteria were 500–1000 µg/mL, so it had antibacterial activity.