﻿AbstractThe genusApiosporaincludes endophytes, pathogens and saprobes, with a wide host range and geographic distribution. In this paper, sixApiosporastrains isolated from diseased and healthy tissues of bamboo leaves from Hainan and Shandong provinces in China were classified using a multi-locus phylogeny based on a combined dataset ofITS,LSU,tef1andtub2, in conjunction with morphological characters, host association and ecological distribution. Two new species,ApiosporadongyingensisandA.hainanensis, and a new record ofA.pseudosinensisin China, are described based on their distinct phylogenetic relationships and morphological analyses. Illustrations and descriptions of the three taxa are provided, along with comparisons with closely related taxa in the genus.