Acoustical properties of various materials were analyzed in order to determine their potential for the utilization in the three-dimensional printing process of stringed musical instruments. Polylactic acid (PLA), polyethylene terephthalate with glycol modification (PET-G), and acrylonitrile styrene acrylate (ASA) filaments were studied in terms of sound reflection using the transfer function method. In addition, the surface geometry parameters (Sa, Sq, Sz, and Sdr) were measured, and their relation to the acoustic performance of three-dimensional-printed samples was investigated. It was found that a higher layer height, and thus a faster printing process, does not necessarily mean poor acoustical properties. The proposed methodology also proved to be a relatively easy and rapid way to test the acoustic performance of various materials and the effect of three-dimensional printing parameters to test such a combination at the very beginning of the production process.