Simple SummaryTapeworm infections are among the most relevant parasitic diseases in both human and animal health. Several tapeworms rely on wild animals to complete their life cycle, among them, taeniids from the GenusEchinococcusare particularly important as they are the causative agents of cystic and alveolar echinococcosis. The study’s aim was to perform a molecular screening of fecal samples collected from carcasses of wild carnivores from Central Italy using a multiplex PCR and Sanger sequencing approach. Out of 279 samples, 134 tested positive for eitherTaeniaspp. OrEchinococcus granulosussensu lato. Sanger sequencing was performed on every positive sample to produce a taxonomical attribution of the parasitic DNA.Echinococcus granulosussensu stricto (genotype G3) was detected in only one Apennine wolf, whereas no sample tested positive forE. multilocularis. Other tapeworms that were commonly found in the sample pool were:Mesocestoides corti(syn.M. vogae),M. litteratus,Taenia serialis, andT. hydatigena. The results of the survey suggest thatEchinococcusinfections in the study area do not seem to be sustained by sylvatic cycles. The survey corroborates, yet again, the importance of passive surveillance of wild animals that can serve as reservoirs for zoonotic pathogens.AbstractTapeworm infections are among the most relevant parasitic diseases in humans and animals. Tapeworms from the GenusEchinococcusare particularly important as they can cause cystic or alveolar echinococcosis. A molecular screening was performed on 279 fecal samples collected from carcasses of wild carnivores from Central Italy using PCR targeting diagnostic fragments ofnad1,rrnS, andnad5genes. Samples positive for eitherTaeniaspp. orEchinococcus granulosuswere sequenced to taxonomically identify the parasitic DNA. Of the 279 samples, 134 (48.0%) gave positive results in the multiplex PCR. Only one (0.4%) sample from an Apennine wolf tested positive forEchinococcus granulosussensu stricto (genotype G3), whereas no sample tested positive forE. multilocularis. The most frequently detected tapeworms were:Mesocestoides corti(synM. vogae) (12.9%),M. litteratus(10.8%),Taenia serialis(9.3%), andT. hydatigena(6.5%), other tapeworms were rarely detected. The results suggest thatEchinococcusinfections in Central Italy do not seem to be sustained by sylvatic cycles, confirming the absence ofE. multilocularisin Central Italy. The survey corroborates, yet again, the importance of passive surveillance of wild animals that can serve as reservoirs for zoonotic pathogens, especially on wild canids that in other areas are strongly implicated in the transmission ofE. granulosusandE. multilocularis.