Agriculture has been and still is one of the most influential primary operations in economic history worldwide. Its social, cultural, and political impact allows the progression and survival of humanity. Sustaining the supply of primary resources is crucial for the future. Therefore, the development of new technologies applied to agrochemicals is growing to obtain better food quality faster. Recently, nanotechnology has gained strength in this field in the last decade, mainly because of the presumed benefits that will carry with it compared with the current commercial presentations, like the decrease of risk in non-target organisms. The harm of pesticides is commonly associated with unwanted effects on human health, some with long-term genotoxic effects. Therefore, it would be relevant to set the existence of a risk or a benefit of the nanopesticides from a genotoxic point of view, comparing against those without this technology. Although some studies are concerned with its genotoxicity in live aquatic organisms, few focus on human in vitro models. Several studies conclude that some of them can induce oxidative stress, leading to DNA damage or cell death. However, there is still much to investigate to establish an accurate and complete assessment. In this review, we aim to give an overview of the genotoxic effect caused by nanopesticides in animal cells and a guide to the evolution of this topic, offering a base and critical review to facilitate future research.