ObjectiveThis study investigated the effects of aqueous and hydro-alcoholic extracts
ofSeidlitzia rosmarinuson reproductive hormones, sperm
variables, and antioxidant enzymes level in the mice testis.MethodsIn this experimental study, 24 three-month-old male NMRI mice weighing
(25-30g) were divided into three groups: control, aqueous and
hydro-alcoholic extracts ofSeidlitzia rosmarinus100mg/kg.
Dissolved extracts were gavaged orally for 35 days. One day after receiving
the last dose of the extract, the blood sample, testis, and the epididymis
tail were taken for plasma hormonal, testicular antioxidants level, sperm
count, and vitality assessments.ResultsTesticular level of malondialdehyde increased in aqueous and hydro-alcoholic
extracts groups (p=0.04); total antioxidant capacity
decreased in aqueous and hydro-alcoholic extracts groups
(p=0.008); and the consumption of aqueous
(p<0.001) and hydro-alcoholic
(p=0.03) extracts decreased catalase in comparison with the
control group. The plasma level of luteinizing hormone decreased in the
aqueous extracts administrated group (p=0.009); the
follicle-stimulating hormone increased in aqueous (p=0.03),
and hydro-alcoholic extracts administered mice; and the testosterone level
decreased in aqueous extract-treated animals versus the control group
(p<0.001). The sperm count was increased in aqueous
(p=0.04) and hydro-alcoholic (p=0.009)
extracts groups, but its vitality was decreased (p=0.008)
in comparison with the control group.ConclusionsIn conclusion,Seidlitzia rosmarinushas an adverse effect
on male reproductive hormones and sperm viability via increased lipid
peroxidation and reduced antioxidant defense system performance.