BackgroundEnvironmental stress due to climate or pathogens is a major threat to modern agriculture. Plant genetic resistance to these stresses is one way to develop more resilient crops, but accurately quantifying plant phenotypic responses can be challenging. Here we develop and test a set of metrics to quantify plant wilting, which can occur in response to abiotic stress such as heat or drought, or in response to biotic stress caused by pathogenic microbes. These metrics can be useful in genomic studies to identify genes and genomic regions underlying plant resistance to a given stress.ResultsWe use two datasets: one of tomatoes inoculated withRalstonia solanacearum, a soilborne pathogen that causes bacterial wilt disease, and another of soybeans exposed to water stress. For both tomato and soybean, the metrics predict the visual wilting score provided by human experts. Specific to the tomato dataset, we demonstrate that our metrics can capture the genetic difference of bacterium wilt resistance among resistant and susceptible tomato genotypes. In soybean, we show that our metrics can capture the effect of water stress.ConclusionOur proposed RGB image-based wilting metrics can be useful for identifying plant wilting caused by diverse stresses in different plant species.