Aedes aegyptiandAnopheles stephensihave challenged human health by transmitting several infectious disease agents, such as malaria, dengue fever, and yellow fever. Larvicides, especially in endemic regions, is an effective approach to the control of mosquito-borne diseases. In this study, the composition of three essential oil from theArtemisiaL. family was analyzed by Gas Chromatography–Mass Spectrometry. Afterward, nanoliposomes containing essential oils ofA. annua,A. dracunculus, andA. sieberiwith particle sizes of 137 ± 5, 151 ± 6, and 92 ± 5 nm were prepared. Besides, their zeta potential values were obtained at 32 ± 0.5, 32 ± 0.6, and 43 ± 1.7 mV. ATR-FTIR analysis (Attenuated Total Reflection-Fourier Transform InfraRed) confirmed the successful loading of the essential oils. Moreover, The LC50values of nanoliposomes againstAe. aegyptilarvae were 34, 151, and 197 µg/mL. These values forAn.stephensiwere obtained as 23 and 90, and 140 µg/mL, respectively. The results revealed that nanoliposomes containingA. dracunculusexerted the highest potential larvicidal effect againstAe. aegyptiandAn. stephensi,which can be considered against other mosquitoes.