Background:Training machine learning (ML) models in medical imaging requires large amounts of labeled data. To minimize labeling workload, it is common to divide training data among multiple readers for separate annotation without consensus and then combine the labeled data for training a ML model. This can lead to a biased training dataset and poor ML algorithm prediction performance. The purpose of this study is to determine if ML algorithms can overcome biases caused by multiple readers’ labeling without consensus.Methods:This study used a publicly available chest X-ray dataset of pediatric pneumonia. As an analogy to a practical dataset without labeling consensus among multiple readers, random and systematic errors were artificially added to the dataset to generate biased data for a binary-class classification task. The Resnet18-based convolutional neural network (CNN) was used as a baseline model. A Resnet18 model with a regularization term added as a loss function was utilized to examine for improvement in the baseline model.Results:The effects of false positive labels, false negative labels, and random errors (5–25%) resulted in a loss of AUC (0–14%) when training a binary CNN classifier. The model with a regularized loss function improved the AUC (75–84%) over that of the baseline model (65–79%).Conclusion:This study indicated that it is possible for ML algorithms to overcome individual readers’ biases when consensus is not available. It is recommended to use regularized loss functions when allocating annotation tasks to multiple readers as they are easy to implement and effective in mitigating biased labels.