According to the Food and Agriculture Organization of the United Nations, pork remains the most consumed meat in the world. Consequently, it is very important to ensure that it is of the highest microbiological quality. Many of the pathogens that cause lymph node lesions in pigs are zoonotic agents, and the most commonly isolated bacteria areMycobacteriumspp.,Streptococcusspp.,Staphylococcus aureusandRhodococcus equi(synonymous withPrescottella equi). The prevention and treatment of zoonotic infections caused by these bacteria are mainly based on antimicrobials. However, an overuse of antimicrobials contributes to the emergence and high prevalence of antimicrobial-resistant strains, which are becoming a serious challenge in many countries. The aim of this study was to evaluate the genetic diversity and antimicrobial resistance of theStreptococcusspp. (n= 48),S. aureus(n= 5) andR. equi(n= 17) strains isolated from swine lymph nodes with and without lesions. All isolates ofS. dysgalactiae,S. aureusandR. equiwere subjected to PFGE analysis, which showed the genetic relatedness of the tested bacteria in the studied pig populations. Additionally, selected tetracycline and macrolide resistance genes in the streptococcal strains were also studied. The results obtained in the present study provide valuable data on the prevalence, diversity, and antimicrobial resistance of the studied bacteria. Numerous isolated bacterialStreptococcusspp. strains presented resistance to doxycycline, and almost half of them carried tetracycline resistance genes. In addition,R. equiandS. aureusbacteria presented a high level of resistance to beta-lactam antibiotics and to cefotaxime, respectively.