The pathogenic yeastPichia kudriavzevii, previously known asCandida krusei, is more distantly related toCandida albicansthan clinically relevant CTG-cladeCandidaspecies. Its cell wall, a dynamic organelle that is the first point of interaction between pathogen and host, is relatively understudied, and its wall proteome remains unidentified to date. Here, we present an integrated study of the cell wall inP.kudriavzevii. Our comparative genomic studies and experimental data indicate that the general structure of the cell wall inP.kudriavzeviiis similar toSaccharomyces cerevisiaeandC.albicansand is comprised of β-1,3-glucan, β-1,6-glucan, chitin, and mannoproteins. However, some pronounced differences withC.albicanswalls were observed, for instance, higher mannan and protein levels and altered protein mannosylation patterns. Further, despite absence of proteins with high sequence similarity toCandidaadhesins, protein structure modeling identified eleven proteins related to flocculins/adhesins inS.cerevisiaeorC.albicans. To obtain a proteomic comparison of biofilm and planktonic cells,P.kudriavzeviicells were grown to exponential phase and in static 24-h cultures. Interestingly, the 24-h static cultures ofP.kudriavzeviiyielded formation of floating biofilm (flor) rather than adherence to polystyrene at the bottom. The proteomic analysis of both conditions identified a total of 33 cell wall proteins. In line with a possible role in flor formation, increased abundance of flocculins, in particular Flo110, was observed in the floating biofilm compared to exponential cells. This study is the first to provide a detailed description of the cell wall inP.kudriavzeviiincluding its cell wall proteome, and paves the way for further investigations on the importance of flor formation and flocculins in the pathogenesis ofP.kudriavzevii.