AbstractIn this study, we sequenced and reported the complete mitochondrial genomes ofKusala populifor the first time. The complete mitochondrial genome was registered in GenBank with accession number NC_064377 as the first complete mitogenome of the genusKusala.The circular mitochondrial genome length is 15,402 bp, with nucleotide composition A (41.8%), C (11.4%), G (9.2%), T (37.6%), A + T (79.4%), and C + G (20.6%), comprising 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a D-loop region. All protein-coding genes were encoded by the H-strand, except for 4 genes (nad5,nad4,nad4L,nad1). 8 tRNA genes (tRNA-Gln,tRNA-Cys,tRNA-Tyr,tRNA-Phe,tRNA-His,tRNA-Pro,tRNA-Leu,tRNA-Val)and 2 rRNA genes (16S,12S) were encoded in the L-strand. Phylogenetic analysis indicated that the newly sequenced species had a close relationship withMitjaevia, another widespread Old-World genus of Erythroneurini.