Ticks and tick-borne diseases affect livestock productivity and cause significant economic losses. Therefore, surveillance of these pathogens and vectors is paramount to reducing these effects in livestock. This study aimed to identifyAnaplasma marginaleandBorrelia burgdorferisensu lato in ticks collected from cattle. Molecular biology techniques were utilized to identifyA. marginalefor both types of samples, i.e., ticks and bovine blood. Serology of cattle using indirect immunofluorescence assay (IFA) was conducted to determine antibodies toB.burgdorferis.l. from seven locations in Nuevo Leon, Mexico, between 2015 and 2017. From 404 bovines, 2880 ticks were collected:Rhipicephalus microplus(2391 females and 395 males),Amblyommaspp. (51 females and 42 males) andDermacentor variabilis(1 female).Rhipicephalus microplusrepresented the largest specimens captured, with 96.7% within the seven study sites. PCR processed only 15% (442) of tick samples to identifyA. marginale. Field genera proportions were followed to select testing tick numbers. Results showed that 9.9% (44/442) ofA. maginaleinfected the pooled tick species, whereas the highest percent corresponded to 9.4% (38/404) inR. microplus. Regarding the molecular analysis of blood samples, 214 of 337 (63.5%) were positive forA. maginale. In each of the seven locations, at least one bovine sample tested positive forA. maginale.Borrelia burgdorferis.l. was not found either in the ticks or serum samples. TwoA.marginaleDNA nucleotide sequences obtained in this study were deposited in the GenBank with the following accession numbers OR050501 cattle, and OR050500R.microplustick. Results of this work point to current distribution of bovine anaplasmosis in northern Mexico.