Introduction:The study aims to explore whether NIRS derived data can be used to identify the second ventilatory threshold (VT2) during a maximal incremental treadmill test in non-professional runners and to determine if there is a correlation between SmO2and other valid and reliable exercise performance assessment measures or parameters for maximal incremental test, such as lactate concentration (LT), RPE, HR, and running power (W).Methods:24 participants were recruited for the study (5 women and 19 men). The devices used consisted of the following: i) a muscle oxygen saturation analyzer placed on the vastus lateralis of the right leg, ii) the Stryd power meter for running, iii) the Polar H7 heart rate band; and iv) the lactate analyzer. In addition, a subjective perceived exertion scale (RPE 1-10) was used. All of the previously mentioned devices were used in a maximal incremental treadmill test, which began at a speed of 8 km/h with a 1% slope and a speed increase of 1.2 km/h every 3 min. This was followed by a 30-s break to collect the lactate data between each 3-min stage. Spearman correlation was carried out and the level of significance was set atp< 0.05.Results:The VT2 was observed at 87,41 ± 6,47% of the maximal aerobic speed (MAS) of each participant. No relationship between lactate data and SmO2values (p= 0.076;r= −0.156) at the VT2 were found. No significant correlations were found between the SmO2variables and the other variables (p> 0.05), but a high level of significance and strong correlations were found between all the following variables: power data (W), heart rate (HR), lactate concentration (LT) and RPE (p< 0.05;r> 0.5).Discussion:SmO2data alone were not enough to determine the VT2, and there were no significant correlations between SmO2and the other studied variables during the maximal incremental treadmill test. Only 8 subjects had a breakpoint at the VT2 determined by lactate data.Conclusion:The NIRS tool, Humon Hex, does not seem to be useful in determining VT2 and it does not correlate with the other variables in a maximal incremental treadmill test.