The genusPaenibacilluscontains a variety of biologically active compounds that have potential applications in a range of fields, including medicine, agriculture, and livestock, playing an important role in the health and economy of society. Our study focused on the bacterium SS4T(KCTC 43402T= GDMCC 1.3498T), which was characterized using a polyphasic taxonomic approach. This strain was analyzed using antiSMASH, BAGEL4, and PRISM to predict the secondary metabolites. Lassopeptide clusters were found using all three analysis methods, with the possibility of secretion. Additionally, PRISM found three biosynthetic gene clusters (BGC) and predicted the structure of the product. Genome analysis indicated that glucoamylase is present in SS4T. 16S rRNA sequence analysis showed that strain SS4Tmost closely resembledPaenibacillus marchantiophytorumDSM 29850T(98.22%),Paenibacillus nebraskensisJJ-59T(98.19%), andPaenibacillus acerisKCTC 13870T(98.08%). Analysis of the 16S rRNA gene sequences and Type Strain Genome Server (TYGS) analysis revealed that SS4Tbelongs to the genusPaenibacillusbased on the results of the phylogenetic analysis. As a result of the matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) results, SS4Twas determined to belong to the genusPaenibacillus. ComparingP. marchantiophytorumDSM 29850Twith average nucleotide identity (ANI 78.97%) and digital DNA-DNA hybridization (dDDH 23%) revealed values that were all less than the threshold for bacterial species differentiation. The results of this study suggest that strain SS4Tcan be classified as aPaenibacillus andongensisspecies and is a novel member of the genusPaenibacillus.