Mites of the genusTyrophagus(Acari: Acaridae) are among the most widely distributed mites. The species in this genus cause damage to stored products and crops, and pose a threat to human health. However, the influence ofTyrophagusspp. in apiculture remains unknown. In 2022, a study focusing on the identification ofTyrophagusspecies within five apiaries was conducted in Chungcheongnam Province, Republic of Korea. Its specific objective was to investigate the presence ofTyrophagusmites in response to the reported high mortality of honey bee colonies in this area. Morphological identification and phylogenetic analysis using the mitochondrial gene cytochrome-c oxidase subunit 1 (CO1) confirmed for the first time the presence of the mite speciesTyrophagus curvipenisin a honey bee colony in the Republic of Korea. Two honey bee pathogens were detected in the mite, a viral pathogen (deformed wing virus, DWV) and a protozoal pathogen (Trypanosomaspp.). The presence of the two honey bee pathogens in the mite suggests that this mite could contribute to the spread of related honey bee diseases. However, the direct influence of the miteT. curvipenison honey bee health remains unknown and should be further investigated.