The present study describes the anti-biofilm and quorum sensing (QS) inhibitory potential of extracts and chemical constituents fromPiper bogotense. Antibiofilm potential was determined through crystal violet assay againstPseudomonas aeruginosa, while QS inhibition efficacy was determined through violacein inhibition assay usingChromobacterium violaceumas a bacterial model. Additionally, this study reports the effects of the chemical constituents isolated inP. bogotenseagainst various virulent factors associated with QS, such as the percentage decrease in pyocyanin, elastase, and protease production. The chemical study led to the isolation and identification of two prenylated benzoic acids (1and2) and a prenylated hydroquinone3, of which compounds1and2are reported for the first time forP. bogotense. The ethanolic extract and the DCM fraction fromP. bogotensestand out for reducing violacein production inC. violaceum, as well as the biofilm formation inP. aeruginosa. Compounds2and3stand out for having the lowest violacein production (43.8% and 68.3%), as well as the lowest production of virulence factors such as elastase (60.2% and 51.4%) and pyocyanin (39.7% and 33.2%). These results demonstrate the potential ofP. bogotensecomponents to be used as an alternative control against multidrug-resistantP. aeruginosa.