Background: The rhizome ofLanguas galangalis traditionally used in Sri Lanka for the treatment of skin infections caused by fungi. The aim of the present study was to evaluate the antifungal activity ofL. galangalrhizome and to develop a topical antifungal formulation from it.Methods:The dried, powdered rhizome ofL. galangalwas successively extracted with hexane, dichloromethane, ethyl acetate and methanol using Soxhlet extraction. The agar well diffusion method was used to assess the antifungal activity againstCandida albicansandAspergillus nger. The antifungal activities of the extracts were compared with clotrimazole as the positive control and dimethyl sulfoxide (DMSO) as the negative control. The most active hexane extract was used to prepare the cream. The antifungal activity of the formulated cream was tested.Results:The hexane extract ofL. galangalrhizome powder was more effective onC. albicansandA. niger. The hexane extract ofL. galangalshowed the maximum zone of inhibition againstC. albicansandA. niger(20.20 mm ± 0.46, 18.20 mm ± 0.46) compared to the other three extracts, while clotrimazole, which was used as a positive control, produced a larger zone of inhibition (36.10 mm ± 0.65) and dimethyl sulfoxide (DMSO), the negative control, did not produce inhibitory zones. Stability testing of the formulated cream showed a stable and good appearance.Conclusions:The cream developed using the hexane extract showed in vitro antifungal activity againstC. albicansandA. niger.Further evaluations on shelf life, stability and safety are required.