Necrotizing infections of deep neck spaces are a group of life-threatening infectious diseases acquired through trauma or as a descending infection from an odontogenic source. The isolation of pathogens is unusual because of the anaerobic nature of the infection; however, one way to achieve this is through the use of automated microbiological methods like matrix-assisted laser desorption/ionization and time-of-flight (MALDI-TOF) following standard microbiology protocols for analyzing samples from potential anaerobic infections.We present a case of a patient without risk factors for descending necrotizing mediastinitis with isolationof Streptococcusanginosus and Prevotella​​​​​​​buccaemanaged at the intensive care unit with a multidisciplinary team. We present our approach and how we successfully treat this complicated infection.