ObjectiveTo evaluate the mean uterine artery pulsatility index (UtAPI) in each trimester of pregnancy as a predictor of early or late pre-eclampsia (PE) in Colombian pregnant women.MethodsThe UtAPI was measured in singleton pregnancies in each trimester. Uterine artery pulsatility index as predictor of PE was evaluated by odds ratio (OR), receiver operating characteristic (ROC) curves, and Kaplan-Meier diagram.ResultsAnalysis in the 1stand 3rdtrimester showed that abnormal UtAPI was associated with early PE (OR: 5.99: 95% confidence interval [CI]: 1.64–21.13; and OR: 10.32; 95%CI: 2.75–42.49, respectively). Sensitivity and specificity were 71.4 and 79.6%, respectively, for developing PE (area under the curve [AUC]: 0.922). The Kaplan-Meier curve showed that a UtAPI of 0.76 (95%CI: 0.58–1.0) in the 1sttrimester was associated with early PE, and a UtAPI of 0.73 (95%CI: 0.55–0.97) in the 3rdtrimester was associated with late PE.ConclusionUterine arteries proved to be a useful predictor tool in the 1stand 3rdtrimesters for early PE and in the 3rdtrimester for late PE in a pregnant population with high prevalence of PE.