In the current work, copper tungsten oxide (CuWO4) nanoparticles are incorporated with carbon nanofiber (CNF) to form CNF/CuWO4nanocomposite through a facile hydrothermal method. The prepared CNF/CuWO4composite was applied to the electrochemical detection of hazardous organic pollutants of 4-nitrotoluene (4-NT). The well-defined CNF/CuWO4nanocomposite is used as a modifier of glassy carbon electrode (GCE) to form CuWO4/CNF/GCE electrode for the detection of 4-NT. The physicochemical properties of CNF, CuWO4, and CNF/CuWO4nanocomposite were examined by various characterization techniques, such as X-ray diffraction studies, field emission scanning electron microscopy, EDX-energy dispersive X-ray microanalysis, and high-resolution transmission electron microscopy. The electrochemical detection of 4-NT was evaluated using cyclic voltammetry (CV) the differential pulse voltammetry detection technique (DPV). The aforementioned CNF, CuWO4, and CNF/CuWO4materials have better crystallinity with porous nature. The prepared CNF/CuWO4nanocomposite has better electrocatalytic ability compared to other materials such as CNF, and CuWO4. The CuWO4/CNF/GCE electrode exhibited remarkable sensitivity of 7.258 μA μM−1cm−2, a low limit of detection of 86.16 nM, and a long linear range of 0.2–100 μM. The CuWO4/CNF/GCE electrode exhibited distinguished selectivity, acceptable stability of about 90%, and well reproducibility. Meanwhile, the GCE/CNF/CuWO4electrode has been applied to real sample analysis with better recovery results of 91.51 to 97.10%.