AbstractGut microbiome is critical to the health of mammals. Many previous studies have revealed the gut bacterial microbiomes of mother and infant changed significantly during the weaning period. However, little is known concerning the gut mycobiome of wild primates. Here, we examined the variations on gut mycobiome between weaning and post‐weaning for both mother and infant in wild‐living Tibetan macaques (Macaca thibetana). Our results showed that the gut mycobiomes of mother and infant were dominated by two phyla Ascomycota and Basidiomycota. For both mother and infant, the ASV richness of gut mycobiome remained relatively steady from weaning to post‐weaning periods, while the Shannon indexes increased significant in weaning compared to post‐weaning periods. However, no significant difference between mother and infant ASV richness and Shannon indexes during weaning and post‐weaning periods respectively. Compared to mothers, we found that much more known taxa of gut fungi were enriched in weaning or post‐weaning periods of infants. In particular, we found that the dominant genusAspergilluswas enriched in infants during weaning period. Furthermore, we found that the relative abundance of plant pathogens were significantly higher in the post‐weaning period than in the weaning period for infants. Our results indicated that weaning events could affect the gut mycobiome significantly for both mothers and infant in Tibetan macaques, which had a stronger effect on the gut mycobiome of infant monkeys than on their mothers.