The amount of food waste throughout the world has become quite alarming and is contributing to lower food resources. The study aimed to extract and characterize the polyphenols from corn silks at immature and mature stages through conventional and green extraction techniques. Purposely, corn silks, which are some of the by-products of corn, (Zea maysL.) were collected and subjected to proximate analysis including moisture, ash, protein, fiber, and minerals. Secondly, the antioxidants from both immature and mature corn silks were extracted by techniques involving supercritical and ultrasound extraction alongside conventional extraction. The results displayed a promising quantity of protein and fiber along with calcium, magnesium, sodium potassium, and copper. Among the extraction techniques, supercritical extraction at 3,000 Pa acquired the highest total phenolic contents (TPC), total flavonoids (TF), 2, 2-diphenylpicrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) activities as 128.08 ± 3.74 mg GAE/100 g, 86.73 ± 2.75 mg CE/100 g, 106.73 ± 5.10%, and 73.52 ± 2.33 μM Fe + 2/g, respectively, followed by the ultrasound and conventional extraction techniques. Between the immature and mature corn silks, the highest antioxidant activity was displayed by immature corn silks.