AbstractTartary buckwheat flavonoids have a variety of effects on anti‐inflammatory, anti‐oxidation, as well as anti‐tumor and are valuable for academic research and industrial application.Helicobacter pylori(H.pylori) infection is associated with various gastrointestinal diseases in humans, and an increase in its resistance has led to the failure of many drugs. In this study, we quantified the main monomers of tartary buckwheat (Fagopyrum Tataricum(L.) Gaertn.) bran flavonoids extract through HPLC analysis. Then, we investigated the anti‐H.pyloriactivity and the effect on cell inflammation of tartary buckwheat flavonoids extract and its four main flavonoid monomers (rutin, quercetin, kaempferol, and nicotiflorin). The results showed that tartary buckwheat flavonoids extract and its four flavonoid monomers could inhibit the growth ofH.pyloriand down‐regulate the expression of proinflammatory factors IL‐6, IL‐8, and CXCL‐1 inH.pylori‐induced GES‐1 cells. Moreover, we also confirmed that tartary buckwheat flavonoids extract could reduce the expression of virulence factor gene ofH.pylori. In summary, tartary buckwheat can alleviate the cell inflammation induced byH.pylori, which provides a theoretical basis for the development of tartary buckwheat healthcare products.