In viticulture, the highly resistant rootstock ‘Beta’ is widely used in Chinese grape production to avoid the effects of soil salinization and drought on grape growth. However, the mechanism of high resistance to abiotic stress in the ‘Beta’ rootstock is not clear. In this study, we demonstrated thatVhMYB2as a transcription factor made a significant contribution to salinity and drought stress, which was isolated from the ‘Beta’ rootstock. The coding sequence of theVhMYB2gene was 858 bp, encoding 285 amino acids. The subcellular localization of VhMYB2 was located in the nucleus of tobacco epidermal cells. Moreover, RT-qPCR found thatVhMYB2was predominantly expressed in the mature leaf and root of the grape. Under salinity and drought stress, overexpressingVhMYB2showed a higher resistant phenotype and survival rates inA. thalianawhile the transgenic lines had a survival advantage by measuring the contents of proline, chlorophyll, and MDA, and activities of POD, SOD, and CAT, and expression levels of related stress response genes. The results reveal thatVhMYB2may be an important transcription factor regulating ‘Beta’ resistance in response to abiotic stress.