January 2018, Volume 131, Issue 1, pp 183–191 | Cite as

Fine mapping of the genic male-sterile ms 1 gene in Capsicum annuum L.

Key message

The genomic region cosegregating with the genic male-sterile ms 1 gene of Capsicum annuum L. was delimited to a region of 869.9 kb on chromosome 5 through fine mapping analysis. A strong candidate gene, CA05g06780, a homolog of the Arabidopsis MALE STERILITY 1 gene that controls pollen development, was identified in this region.

Genic male sterility caused by the ms 1 gene has been used for the economically efficient production of massive hybrid seeds in paprika (Capsicum annuum L.), a colored bell-type sweet pepper. Previously, a CAPS marker, PmsM1-CAPS, located about 2–3 cM from the ms 1 locus, was reported. In this study, we constructed a fine map near the ms 1 locus using high-resolution melting (HRM) markers in an F2 population consisting of 1118 individual plants, which segregated into 867 male-fertile and 251 male-sterile plants. A total of 12 HRM markers linked to the ms 1 locus were developed from 53 primer sets targeting intraspecific SNPs derived by comparing genome-wide sequences obtained by next-generation resequencing analysis. Using this approach, we narrowed down the region cosegregating with the ms 1 gene to 869.9 kb of sequence. Gene prediction analysis revealed 11 open reading frames in this region. A strong candidate gene, CA05g06780, was identified; this gene is a homolog of the Arabidopsis MALE STERILITY 1 (MS1) gene, which encodes a PHD-type transcription factor that regulates pollen and tapetum development. Sequence comparison analysis suggested that the CA05g06780 gene is the strongest candidate for the ms 1 gene of paprika. To summarize, we developed a cosegregated marker, 32187928-HRM, for marker-assisted selection and identified a strong candidate for the ms 1 gene.

Communicated by Michael J. Havey.

The online version of this article (doi: 10.1007/s00122-017-2995-0) contains supplementary material, which is available to authorized users.

This research was supported by the “Cooperative Research Program for Agriculture Science & Technology Development” (Project no. PJ 01229801), Rural Development Administration, Republic of Korea. We are grateful to Soung-Woo Park for providing paprika F2 segregating seeds (NHSEED, South Korea).

The authors have no conflicts of interest to declare.

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Supplementary material 1 (DOCX 1540 kb)

© Springer-Verlag GmbH Germany 2017

Jeong, K., Choi, D. & Lee, J. Theor Appl Genet (2018) 131: 183. https://doi.org/10.1007/s00122-017-2995-0
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