4, የማቀዝቀዣ ዞን: r3 = 199 °C / ደቂቃ ማዘጋጀት, andC3 = 80 °C በፍጥነት ማቀዝቀዝ ለማድረግ.
4, ummandla okupholisa; yibhekise R3 = 199 °C / ngomzuzu, andC3 = 80 °C ukwenza apholisayo ngokukhawuleza. kwaye wabeka T3 = 0 umzuzu ukwenzela ukugqiba inkqubo jointing yonke.