Effect of protein, probiotic, and symbiotic supplementation on serum biological health markers of molted layers. Effects of Probiotic Supplementation on Hormonal Profiles, Biomarkers of Inflammation and Oxidative Stress in Women With Polycystic Ovary Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial. The effects of probiotic supplementation on biomarkers of inflammation, oxidative stress and pregnancy outcomes in gestational diabetes Effects of Probiotics on Biomarkers of Oxidative Stress and Inflammatory Factors in Petrochemical Workers: A Randomized, Double-blind, Placebo-controlled Trial The effects of probiotic and selenium co-supplementation on parameters of mental health, hormonal profiles, and biomarkers of inflammation and oxidative stress in women with polycystic ovary syndrome Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men; a randomized, double-blinded, placebo-controlled trial World Allergy Organization-McMaster University Guidelines for Allergic Disease Prevention (GLAD-P): Probiotics Are probiotics safe for use during pregnancy and lactation? Probiotic yogurt improves antioxidant status in type 2 diabetic patients.
Dietary zinc was used to induce molt in 200 White Leghorn birds in caged housing at the age of 70 wk . The birds were equally and r and omly allocated to 4 groups each of 50 birds as G1 ( control ; CP 16 % , no supplement ) , G2 ( CP 18 % , no other supplement ) , G3 ( CP 16 % , symbiotic Perfectin : 85 mg•L(-1 ) in drinking water daily ) , and G4 ( CP 16 % , probiotic Protexin : 85 mg•L(-1 ) in drinking water daily ) after the completion of molt . The sampling was conducted 3 times at 5 % production , peak production , and end of production with 15 birds being killed at each sampling from each group to collect the blood for harvesting of serum . Serum health markers including total oxidant status , total antioxidant capacity , homocysteine concentration , and paraoxonase , arylesterase , and ceruloplasmin activity were determined by the prescribed assays . The overall total antioxidant capacity was increased , whereas total oxidant status and homocysteine concentrations were reduced significantly ( P ≤ 0.01 ) in all the supplemented groups compared with the control . The paraoxonase and ceruloplasmin activity were enhanced ( P ≤ 0.01 ) in the supplemented groups compared with the control , and arylesterase activity was increased ( P ≤ 0.01 ) in only G2 compared with the other groups . Although protein supplementation significantly reduced the oxidative stress , supplementation with symbiotic and probiotic also improved the health status by decreasing the oxidative stress in the birds BACKGROUND To the best of our knowledge , data on effects of probiotic administration on hormonal profiles , biomarkers of inflammation and oxidative stress in women with polycystic ovary syndrome ( PCOS ) are scarce . This investigation was conducted to assess the effects of probiotic supplementation on hormonal profiles , biomarkers of inflammation and oxidative stress in women with PCOS . METHODS This r and omized , double-blind , placebo-controlled trial was conducted on 60 women with PCOS , aged 18 - 40 years old . Subjects were r and omly assigned into 2 groups to receive either probiotics or placebo ( n = 30 each group ) for 12 weeks . Metabolic profiles were quantified at baseline and after a 12-week intervention . RESULTS After the 12-week intervention , compared with placebo , probiotic supplementation significantly increased serum sex hormone-binding globulin ( SHBG ) ( + 25.9 ± 32.5 vs. + 0.5 ± 15.6 nmol/L , P < 0.001 ) and plasma total antioxidant capacity ( TAC ) ( + 8.8 ± 120.5 vs. -98.3 ± 246.4 mmol/L , P = 0.04 ) , and significantly decreased serum total testosterone ( -0.2 ± 0.7 vs. + 0.2 ± 0.6 ng/mL , P = 0.03 ) , modified Ferriman-Gallwey ( mF-G ) scores ( -1.7 ± 1.5 vs. -0.2 ± 1.0 , P < 0.001 ) , serum high-sensitivity C-reactive protein ( hs-CRP ) ( -1150.0 ± 1295.2 vs. + 202.5 ± 1426.3 ng/mL , P < 0.001 ) and plasma malondialdehyde ( MDA ) concentrations ( -0.2 ± 0.6 vs. + 0.9 ± 1.3 µmol/L , P < 0.001 ) . We did not observe any detrimental effect of probiotic supplementation on other metabolic profiles . CONCLUSION Overall , probiotic supplementation of PCOS women for 12 weeks had beneficial effects on total testosterone , SHBG , mFG scores , hs-CRP , TAC and MDA levels but did not affect other metabolic profiles Abstract Objective : This study was design ed to evaluate the effects of probiotic supplementation on biomarkers of inflammation , oxidative stress and pregnancy outcomes  among subjects with gestational diabetes ( GDM ) . Methods : This r and omized , double-blind , placebo-controlled clinical trial was done among 60 subjects with GDM who were not on oral hypoglycemic agents . Patients were r and omly allocated to intake either probiotic capsule containing Lactobacillus acidophilus , Lactobacillus casei and Bifidobacterium bifidum ( 2 × 109 CFU/g each ) ( n = 30 ) or placebo ( n = 30 ) for six weeks . Results : Compared with the placebo , probiotic supplementation result ed in significant decreases in fasting plasma glucose ( FPG ) ( −5.3 ± 6.7 vs. + 0.03 ± 9.0 mg/dL , p = .01 ) , serum high-sensitivity C-reactive protein ( hs-CRP ) ( −2.2 ± 2.7 vs. + 0.5 ± 2.4 μg/mL , p < .001 ) , plasma malondialdehyde ( MDA ) concentrations ( −0.1 ± 0.8 vs. + 0.5 ± 1.5 μmol/L , p = .03 ) and MDA/TAC ratio ( −0.0003 ± 0.0008 vs. + 0.0009 ± 0.002 , p = .004 ) , and a significant increase in total antioxidant capacity ( TAC ) levels ( + 65.4 ± 103.3 vs. −37.2 ± 143.7 mmol/L , p = .002 ) . Probiotic supplementation did not affect pregnancy outcomes . Conclusions : Overall , probiotic supplementation among women with GDM for six weeks had beneficial effects on FPG , serum hs-CRP , plasma TAC , MDA and oxidative stress index , but did not affect pregnancy outcomes Background : The aim of the current study was to determine effects of probiotic yoghurt and multispecies probiotic capsule supplementation on biomarkers of oxidative stress and inflammatory factors in petrochemical workers . Methods : This r and omized , double-blind , placebo-controlled trial was done among petrochemical workers . Subjects were r and omly divided into three groups to receive 100 g/day probiotic yogurt ( n = 12 ) or one probiotic capsule daily ( n = 13 ) or 100 g/day conventional yogurt ( n = 10 ) for 6 weeks . The probiotic yoghurt was containing two strains of Lactobacillus acidophilus and Bifidobacterium lactis with a total of min 1 × 107 CFU . Multispecies probiotic capsule contains seven probiotic bacteria spices Actobacillus casei 3 × 103 , L. acidophilus 3 × 107 , Lactobacillus rhamnosus 7 × 109 , Lactobacillus bulgaricus 5 × 108 , Bifidobacterium breve 2 × 1010 , Bifidobacterium longum 1 × 109 and Streptococcus thermophilus 3 × 108 CFU/g . Fasting blood sample s were obtained at the beginning and end of the trial to quantify biomarkers of oxidative stress and inflammatory factors . Results : Although a significant within-group decrease in plasma protein carbonyl levels was seen in the probiotic capsule group ( 326.0 ± 308.9 vs. 251.0 ± 176.3 ng/mL , P = 0.02 ) , the changes were similar among the three groups . In addition , significant within-group decreases in plasma iso prostagl and in were observed in the probiotic supplements group ( 111.9 ± 85.4 vs. 88.0 ± 71.0 pg/mL , P = 0.003 ) and in the probiotic yogurt group ( 116.3 ± 93.0 vs. 92.0 ± 66.0 pg/mL , P = 0.02 ) , nevertheless there were no significant change among the three groups . Conclusions : Taken together , consumption of probiotic yogurt or multispecies probiotic capsule had beneficial effects on biomarkers of oxidative stress in petrochemical workers Background The aim of this study was to evaluate the effect of the co-administration of probiotic and selenium on parameters of mental health , hormonal profiles , and biomarkers of inflammation and oxidative stress in women with PCOS . Data on the effects of selenium and probiotic co-supplementation on mental health , hormonal and inflammatory parameters of patients with polycystic ovary syndrome ( PCOS ) are scarce . This investigation was carried out to evaluate the effects of selenium and probiotic co-supplementation on mental health , hormonal and inflammatory parameters in women with PCOS . Methods This r and omized , double-blinded , placebo-controlled clinical trial was conducted on 60 subjects , aged 18–40 years old . Participants were r and omly allocated into two groups to intake 8 × 109 CFU/day probiotic plus 200 μg/day selenium supplements ( n = 30 ) or placebo ( n = 30 ) for 12 weeks . Hormonal and inflammatory parameters were measured at baseline and after the 12-week intervention . Results Probiotic and selenium co-supplementation result ed in a significant improvement in beck depression inventory ( β − 0.76 ; 95 % CI , − 1.26 , − 0.26 ; P = 0.003 ) , general health question naire scores ( β − 1.15 ; 95 % CI , − 1.97 , − 0.32 ; P = 0.007 ) and depression anxiety and stress scale scores ( β − 1.49 ; 95 % CI , − 2.59 , − 0.39 ; P = 0.009 ) compared with the placebo . Furthermore , probiotic and selenium co-supplementation  significantly reduced total testosterone ( β − 0.26 ng/mL ; 95 % CI , − 0.51 , − 0.02 ; P = 0.03 ) , hirsutism ( β − 0.43 ; 95 % CI , − 0.74 , − 0.11 ; P = 0.008 ) , high-sensitivity C-reactive protein ( hs-CRP ) ( β − 0.58 mg/L ; 95 % CI , − 0.97 , − 0.19 ; P = 0.004 ) and malondialdehyde ( MDA ) levels ( β − 0.29 μmol/L ; 95 % CI , − 0.56 , − 0.02 ; P = 0.03 ) , and significantly increased total antioxidant capacity ( TAC ) ( β + 84.76 mmol/L ; 95 % CI , + 48.08 , + 121.44 ; P < 0.001 ) and total glutathione ( GSH ) levels ( β + 26.78 μmol/L ; 95 % CI , + 4.33 , + 49.23 ; P = 0.02 ) compared with the placebo . Conclusions Overall , the co-administration of probiotic and selenium for 12 weeks to women with PCOS had beneficial effects on mental health parameters , serum total testosterone , hirsutism , hs-CRP , TAC , GSH and MDA levels . This study was prospect ively registered in the Iranian website ( www.i rct .ir ) for registration of clinical trials ( http://www.i rct .ir : I RCT 20170513033941N22).Trial registration I RCT 20170513033941N22 Background Probiotics are an upcoming group of nutraceuticals cl aim ing positive effects on athlete ’s gut health , redox biology and immunity but there is lack of evidence to support these statements . Methods We conducted a r and omized , double-blinded , placebo controlled trial to observe effects of probiotic supplementation on markers of intestinal barrier , oxidation and inflammation , at rest and after intense exercise . 23 trained men received multi-species probiotics ( 1010 CFU/day , Ecologic ® Performance or OMNi-BiOTiC ® POWER , n = 11 ) or placebo ( n = 12 ) for 14 weeks and performed an intense cycle ergometry over 90 minutes at baseline and after 14 weeks . Zonulin and α1-antitrypsin were measured from feces to estimate gut leakage at baseline and at the end of treatment . Venous blood was collected at baseline and after 14 weeks , before and immediately post exercise , to determine carbonyl proteins ( CP ) , malondialdehyde ( MDA ) , total oxidation status of lipids ( TOS ) , tumor necrosis factor-alpha ( TNF-α ) , and interleukin-6 ( IL-6 ) . Statistical analysis used multifactorial analysis of variance ( ANOVA ) . Level of significance was set at p < 0.05 , a trend at p < 0.1 . Results Zonulin decreased with supplementation from values slightly above normal into normal ranges ( < 30 ng/ml ) and was significantly lower after 14 weeks with probiotics compared to placebo ( p = 0.019 ) . We observed no influence on α1-antitrypsin ( p > 0.1 ) . CP increased significantly from pre to post exercise in both groups at baseline and in the placebo group after 14 weeks of treatment ( p = 0.006 ) . After 14 weeks , CP concentrations were tendentially lower with probiotics ( p = 0.061 ) . TOS was slightly increased above normal in both groups , at baseline and after 14 weeks of treatment . There was no effect of supplementation or exercise on TOS . At baseline , both groups showed considerably higher TNF-α concentrations than normal . After 14 weeks TNF-α was tendentially lower in the supplemented group ( p = 0.054 ) . IL-6 increased significantly from pre to post exercise in both groups ( p = 0.001 ) , but supplementation had no effect . MDA was not influenced , neither by supplementation nor by exercise . Conclusions The probiotic treatment decreased Zonulin in feces , a marker indicating enhanced gut permeability . Moreover , probiotic supplementation beneficially affected TNF-α and exercise induced protein oxidation . These results demonstrate promising benefits for probiotic use in trained men . Clinical trial registryhttp://www . clinical trials.gov , identifier : Background Prevalence of  allergic diseases in infants , whose parents and siblings do not have allergy , is approximately 10 % and reaches 20–30 % in those with an allergic first-degree relative . Intestinal microbiota may modulate immunologic and inflammatory systemic responses and , thus , influence development of sensitization and allergy . Probiotics have been reported to modulate immune responses and their supplementation has been proposed as a preventive intervention . Objective The World Allergy Organization ( WAO ) convened a guideline panel to develop evidence -based recommendations about the use of probiotics in the prevention of allergy . Methods We identified the most relevant clinical questions and performed a systematic review of r and omized controlled trials of probiotics for the prevention of allergy . We followed the Grading of Recommendations Assessment , Development and Evaluation ( GRADE ) approach to develop recommendations . We search ed for and review ed the evidence about health effects , patient values and preferences , and re source use ( up to November 2014 ) . We followed the GRADE evidence -to-decision framework to develop recommendations . Results Currently available evidence does not indicate that probiotic supplementation  reduces the risk of developing allergy in children . However , considering all critical outcomes in this context , the WAO guideline panel determined that there is a likely net benefit from using probiotics result ing primarily from prevention of eczema . The WAO guideline panel suggests : a ) using probiotics in pregnant women at high risk for having an allergic child ; b ) using probiotics in women who breastfeed infants at high risk of developing allergy ; and c ) using probiotics in infants at high risk of developing allergy . All recommendations are conditional and supported by very low quality evidence . Conclusions WAO recommendations about probiotic supplementation for prevention of allergy are intended to support parents , clinicians and other health care professionals in their decisions whether to use probiotics in pregnancy and during breastfeeding , and whether to give them to infants QUESTION There has been a great deal of discussion in both the medical and lay literature about the use of probiotics to improve general health . Subsequently , pregnant women have been asking me if probiotics used for treating conditions such as bacterial vaginosis and diarrhea are safe to use during pregnancy and lactation . ANSWER Current data suggest that probiotic supplementation is rarely systemically absorbed when used by healthy individuals . One meta- analysis and several r and omized controlled trials conducted with women during the third trimester of pregnancy did not report an increase in adverse fetal outcomes . There have been no published studies addressing Saccharomyces species use in pregnancy . Probiotics are unlikely to be transferred into breast milk OBJECTIVE Oxidative stress plays a major role in the pathogenesis and progression of diabetes . Among various functional foods with an antioxidant effect , probiotic foods have been reported to repress oxidative stress . The objective of this clinical trial was to assess the effects of probiotic and conventional yogurt on blood glucose and antioxidant status in type 2 diabetic patients . METHODS Sixty-four patients with type 2 diabetes mellitus , 30 to 60 y old , were assigned to two groups in this r and omized , double-blind , controlled clinical trial . The patients in the intervention group consumed 300 g/d of probiotic yogurt containing Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 and those in the control group consumed 300 g/d of conventional yogurt for 6 wk .  Fasting blood sample s , 24-h dietary recalls , and anthropometric measurements were collected at the baseline and at the end of the trial . RESULTS Probiotic yogurt significantly decreased fasting blood glucose ( P < 0.01 ) and hemoglobin A1c ( P < 0.05 ) and increased erythrocyte superoxide dismutase and glutathione peroxidase activities and total antioxidant status ( P < 0.05 ) compared with the control group . In addition , the serum malondialdehyde concentration significantly decreased compared with the baseline value in both groups ( P < 0.05 ) . No significant changes from baseline were shown in insulin concentration and erythrocyte catalase activity within either group ( P > 0.05 ) . CONCLUSION The consumption of probiotic yogurt improved fasting blood glucose and antioxidant status in type 2 diabetic patients . These results suggest that probiotic yogurt is a promising agent for diabetes management
The current meta- analysis revealed that probiotic supplementation may result in increasing TAC and lowering MDA , which improve antioxidant status .
Previous studies have supposed that probiotic supplementation led to a positive effect on different health outcomes .
Furthermore , several studies indicated that probiotics supplementation improved antioxidant status , while some studies did not indicate these effects .
Hence , current systematic review and meta- analysis study was conducted to determine the effect of probiotic supplementation on some oxidative stress biomarkers  among adult subjects .