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url: =LOCUS       Exported                3781 bp ds-DNA    circular SYN 27-12-2012\nDEFINITION  Bacterial vector with a CloDF13 (CDF) origin for the co-expression\nof two genes.\nREFERENCE   1  (bases 1 to 3781)\n/note="Plasmids containing the CloDF13 (CDF) origin of\n601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc\n661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt\n721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat\n781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc\n841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg\n901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg\n961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct\n1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca\n1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt\n1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa\n1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca\n1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca\n1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta\n1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag\n1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca\n1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac\n1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga\n1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa\n1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat\n1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag\n1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct\n1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac\n1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt\n1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc\n2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc\n2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt\n2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc\n2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag\n2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat\n2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta\n2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg\n2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg\n2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg\n2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc\n2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc\n2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg\n2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg\n2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc\n2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca\n2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc\n3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc\n3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc\n3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt\n3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca\n3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga\n3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc\n3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg\n3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt\n3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc\n3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca\n3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga\n3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc\n3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat\n常规 长度 3781\n常规 描述 Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes.\n来源 实验宿主 Escherichia coli 1..3781\n来源 分子类型 other DNA 1..3781\n来源 种属 synthetic DNA construct 1..3781\n编码区 翻译起始位点 1 680..1471\n编码区 基因 aadA 680..1471\n编码区 备注 SmR,confers resistance to spectinomycin and streptomycin 680..1471\n编码区 产物 aminoglycoside adenylyltransferase 680..1471\n编码区 翻译序列 MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK 680..1471\n启动子 基因 bla 1472..1563\n启动子 备注 AmpR promoter 1472..1563\n复制起点 方向 LEFT 1611..2349\n复制起点 备注 CloDF13 ori,Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1611..2349\n编码区 翻译起始位点 1 2559..3641\n编码区 基因 lacI 2559..3641\n编码区 备注 lacI,The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). 2559..3641\n编码区 产物 lac repressor 2559..3641\n编码区 翻译序列 MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ 2559..3641\n启动子 基因 lacI 3642..3719\n启动子 备注 lacI promoter, 3642..3719\n启动子 备注 T7 promoter,promoter for bacteriophage T7 RNA polymerase 2..3765


url: =Balti – Ma Tloumounich Balti – Je t’aime avec Saida. Balti – For My People. Balti – Semi El Pac. Balti – Mahomchi kifna feat Mr Mustapha. Rouicha Mohamed – Awisn arbe.\nBalti – Ya olidi. Balti – 3arbi Gotrip. Rouicha – Inas inas vost. Balti – Mahomchi kifna feat Mr Mustapha. Balti – Fast Line. Cheb Abdelhak – Lat Gol Khouya – top raii.\nRouicha – Bini o bink ya lhbiba. Mohamed Rouicha – Goulou lih.\nMohamed Rouicha – Khouya rfaq biya. Elhorma Noune Awa – Rouicha. Mohamed rouicha – Akhoya Matkrah Ljamal. Bouarfa – Ana khouya.\nIMGA – Mohamed rouicha-nssani nanssak-video mustapha-bouchlih. Rouicha – A3zz Nnass. Ahmed Allah Rouicha – By.\nCheikh chouchou – Ana w khouya – medahate Apoka – Khouya sghir. Rouicha et tadlaouia – Moul koutchi.\nRouicha-inas inas – Rouicha-inas inas tamazgha maroc. Mohamed Rouicha – Inas Inas.\nRouicha Mohamed – Mach ghourbagh. Jimmy Oihid – Khouya mon frere.\nAhmed allah rouicha – Warda ola jarda. Rfa9 ja junun dh chayatin – Abu Chayma. Mohamed Rouicha – Ghass Adyali Aari. Mohamed Rouicha – Allah ejma3e elmoumnine. Rouicha – Yak Ana lik.\nRouicha Ahmed Allah – Inas inas. Mohamed Rouicha – Chehal Addit Melahmoum. Ahmad allah Rouicha – Rouicha – Inas inas vost.\nBaraka Imtawen 9sara – Rouicha. IMGA – Mohamed rouicha-hjarni wra7-video -bouchlih. Salah Sadaoui – Ya khouya mouh. Helmi Weld Hbiba – Khouya ya.\nHatim Officiel – Hommage Mohamed Kkhouya. Chippie El Berkani – Mamjowej Khouya. Ahouzar rend hommage rouicha – Mohamed Rouicha – Song. Ana w khouya – Khouua Cheikha Bakhta – Khouya Ould Oumaa.\nAbdellah Daoudi – 3lach l3dawa ya khouya C’mo. Rouicha Mohamed – Awisn arbe. Cheb Abdelhak – Lat Gol Khouya – top raii. Deejay H-F – Leogarredes house bootleg edit inas inas Rouicha clubbing. Trit Titrit Ayoulinu 9sara – Rouicha et Cherifa.\nBalti Khouya » Telecharger Mp3 Gratuite\nHoba Hoba Spirit Feat. Mohamed Rouicha – Atlas Dancefloor. Rouicha – Tedid Awa Tedid.


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