url: =Xu, Jianqing (27)\nZhang, Xiaoyan (16)\nQiu, Chao (7)\nWan, Yanmin (7)\nMeng, Zhefeng (5)\nBen, Yinyin (4)\nHu, Yunwen (4)\nLi, Liangzhu (4)\nRen, Xiaonan (4)\nTian, Di (4)\nYuan, Songhua (4)\nYuan, Zhenghong (4)\nZhang, Wanju (4)\nQiu, Chenli (3)\nZhu, Zhaoqin (3)\nAbubakar, Yassir F. (2)\nHu, Zhidong (2)\nQiu, Sugan (2)\nRen, Yanqin (2)\nShi, Bisheng (2)\nSong, Zhigang (2)\nWu, Huanmei (2)\nXin, Ruolei (2)\nZhu, Lingyan (2)\nArya, Suresh K. (1)\nBosch, Marnix (1)\nBowman, Gregory D. (1)\nCai, Jialin (1)\nChen, Haili (1)\nChu, Yi Wei (1)\nDid you mean: author:("Xu, jiangling")\t+\n1. Willingness to Participate in HIV Therapeutic Vaccine Trials among HIV-Infected Patients on ART in China Dong, Yuan | Shen, Xiaoxing | Guo, Ruizhang | Liu, Baochi | Zhu, Lingyan | Wang, Jing | Zhang, Linxia | Sun, Jun | Zhang, Xiaoyan | Xu, Jianqing\nPLoS ONE 2014;9(11):e111321.\nThe overall willingness to participate in HIV therapeutic vaccine trials was 91.5%. Interestingly, multivariate logistic regression analyses demonstrated that the willingness was higher for those sexually infected by HIV (odds ratio [OR]: 4.36; 95% confidence interval [CI]: 1.53–12.41), diagnosed as HIV-1 infection for greater than 5 years (OR: 7.12, 95% CI: 1.83–27.76), and with the presence of infectious complications (OR: 2.75; 95% CI: 1.02–7.45). The primary reason for participation was to delay or reduce antiretroviral treatment (ART) and to avoid ART side effects (76.6%), and then followed by delaying disease progression (74.9%), increasing immune response to suppress opportunistic infections (57.7%) and preventing the development of drug resistance (37.1%). Reasons for unwillingness to participate mainly included concern for safety (37.0%), lack of knowledge on therapeutic vaccine (33.3%), and satisfaction with ART effectiveness (22.2%).\n2. Boosting Functional Avidity of CD8+ T Cells by Vaccinia Virus Vaccination Depends on Intrinsic T-Cell MyD88 Expression but Not the Inflammatory Milieu Hu, Zhidong | Wang, Jing | Wan, Yanmin | Zhu, Lingyan | Ren, Xiaonan | Qiu, Sugan | Ren, Yanqin | Yuan, Songhua | Ding, Xiangqing | Chen, Jian | Qiu, Chenli | Sun, Jun | Zhang, Xiaoyan | Xiang, Jim | Qiu, Chao | Xu, Jianqing\nJournal of Virology 2014;88(10):5356-5368.\nT-cell functional avidity is a crucial determinant for efficient pathogen clearance. Although recombinant DNA priming coupled with a vaccinia-vectored vaccine (VACV) boost has been widely used to mount robust CD8+ T-cell responses, how VACV boost shapes the properties of memory CD8+ T cells remains poorly defined. Here, we characterize the memory CD8+ T cells boosted by VACV and demonstrate that the intrinsic expression of MyD88 is critical for their high functional avidity. Independent of selection of clones with high-affinity T-cell receptor (TCR) or of enhanced proximal TCR signaling, the VACV boost significantly increased T-cell functional avidity through a decrease in the activation threshold. VACV-induced inflammatory milieu is not sufficient for this improvement, as simultaneous administration of the DNA vaccine and mock VACV had no effects on the functional avidity of memory CD8+ T cells. Furthermore, reciprocal adoptive transfer models revealed that the intrinsic MyD88 pathway is required for instructing the functional avidity of CD8+ T cells boosted by VACV. Taking these results together, the intrinsic MyD88 pathway is required for the high functional avidity of VACV-boosted CD8+ T cells independent of TCR selection or the VACV infection-induced MyD88-mediated inflammatory milieu.\nIMPORTANCE Functional avidity is one of the crucial determinants of T-cell functionality. Interestingly, although it has been demonstrated that a DNA prime-VACV boost regimen elicits high levels of T-cell functional avidity, how VACV changes the low avidity of CD8+ T cells primed by DNA into higher ones in vivo is less defined. Here, we proved that the enhancement of CD8+ T cell avidity induced by VACV boost is mediated by the intrinsic MyD88 pathway but not the MyD88-mediated inflammatory milieu, which might provide prompts in vaccine design.\n3. Drug susceptibility profile and pathogenicity of H7N9 influenza virus (Anhui1 lineage) with R292K substitution Zhang, Xiaonan | Song, Zhigang | He, Jing | Yen, Hui-Ling | Li, Jianhua | Zhu, Zhaoqin | Tian, Di | Wang, Wei | Xu, Lei | Guan, Wencai | Liu, Yi | Wang, Sen | Shi, Bisheng | Zhang, Wanju | Qin, Boyin | Cai, Jialin | Wan, Yanmin | Xu, Chunhua | Ren, Xiaonan | Chen, Haili | Liu, Lu | Yang, Yuqin | Zhou, Xiaohui | Zhou, Wenjiang | Xu, Jianqing | Zhang, Xiaoyan | Peiris, Malik | Hu, Yunwen | Yuan, Zhenghong\n4. Glioma-Associated Antigen HEATR1 Induces Functional Cytotoxic T Lymphocytes in Patients with Glioma Wu, Zhe Bao | Qiu, Chao | Zhang, An Li | Cai, Lin | Lin, Shao Jian | Yao, Yu | Tang, Qi Sheng | Xu, Ming | Hua, Wei | Chu, Yi Wei | Mao, Ying | Zhu, Jian Hong | Xu, Jianqing | Zhou, Liang Fu\n5. Structure-based non-canonical amino acid design to covalently crosslink an antibody–antigen complex Xu, Jianqing | Tack, Drew | Hughes, Randall A. | Ellington, Andrew D. | Gray, Jeffrey J.\n6. Extending RosettaDock with water, sugar, and pH for prediction of complex structures and affinities for CAPRI rounds 20–27 Kilambi, Krishna Praneeth | Pacella, Michael S. | Xu, Jianqing | Labonte, Jason W. | Porter, Justin R. | Muthu, Pravin | Drew, Kevin | Kuroda, Daisuke | Schueler-Furman, Ora | Bonneau, Richard | Gray, Jeffrey J.\nCAPRI; protein interactions; protein docking; binding\n7. Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA Priming/Intramuscular Recombinant Vaccinia Boosting Regimen Qiu, Sugan | Ren, Xiaonan | Ben, Yinyin | Ren, Yanqin | Wang, Jing | Zhang, Xiaoyan | Wan, Yanmin | Xu, Jianqing\nJournal of Immunology Research 2014;2014:308732.\nPrevious study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses. To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV) vaccines expressing OVA-CTB fusion antigen. Female C57BL/6 mice were immunized with an intranasal DNA priming/intramuscular rTTV boosting regimen. OVA specific T-cell responses were measured by IFN-γ ELISPOT and specific antibody responses were determined by ELISA. Compared to the nonadjuvant group (pSV-OVA intranasal priming/rTTV-OVA intramuscular boosting), pSV-OVA-CTB intranasal priming/rTTV-OVA-CTB intramuscular boosting group significantly improved the magnitudes of T-cell responses at spleen (1562 ± 567 SFCs/106 splenocytes versus 330 ± 182 SFCs/106 splenocytes, P < 0.01), mesenteric LN (96 ± 83 SFCs/106 lymphocytes versus 1 ± 2 SFCs/106 lymphocytes, P < 0.05), draining LNs of respiratory tract (109 ± 60 SFCs/106 lymphocytes versus 2 ± 2 SFCs/106 lymphocytes, P < 0.01) and female genital tract (89 ± 48 SFCs/106 lymphocytes versus 23 ± 21 SFCs/106 lymphocytes, P < 0.01). These results collectively demonstrated that fusion-expressed CTB could act as a potent adjuvant to improve both systemic and mucosal T-cell responses.\n8. Differential Compartmentalization of HIV-Targeting Immune Cells in Inner and Outer Foreskin Tissue Liu, Aiping | Yang, Yu | Liu, Lu | Meng, Zhefeng | Li, Liangzhu | Qiu, Chao | Xu, Jianqing | Zhang, Xiaoyan\nPLoS ONE 2014;9(1):e85176.\nEx vivo foreskin models have demonstrated that inner foreskin is more susceptible to HIV-1 infection than outer foreskin. In the present study we characterized the compartition of HIV-1 target cells and quantified these cells in the epidermis and dermis of inner and outer foreskins using immunohistochemistry and flow cytometry. Our data showed that the epidermis of the inner foreskin was more enriched with CD4+ T cells and Langerhans cells (LCs), with the co-expression of CCR5 and α4β7 receptors, than the outer foreskin. Interestingly, the vast majority of CD4+ T cells and LCs expressed CCR5, but not CXCR4, indicating that the inner foreskin might capture and transmit R5-tropic HIV strains more efficiently. In addition, lymphoid aggregates, composed of T cells, macrophages and dendritic cells (DCs) in the dermis, were closer to the epithelial surface in the inner foreskin than in the outer foreskin. As dendritic cells are able to capture and pass HIV particles to susceptible target cells, HIV may be able to more efficiently infect the inner foreskin by hijacking the augmented immune communication pathways in this tissue. After the inoculation of HIV-1 particles in a foreskin explant culture model, the level of p24 antigen in the supernatant from the inner foreskin was slightly higher than that from the outer foreskin, although this difference was not significant. The present study is the first to employ both CCR5 and α4β7 to identify HIV target cells in the foreskin. Our data demonstrated that the inner foreskin was more enriched with HIV target immune cells than the outer foreskin, and this tissue was structured for efficient communication among immune cells that may promote HIV transmission and replication. In addition, our data suggests the R5-tropism of HIV sexual transmission is likely shaped through the inherent receptor composition on HIV target cells in the mucosa.\n9. Five New CRF07_BC Near Full-Length Sequences Isolated from Sichuan, China Meng, Zhefeng | Xin, Ruolei | F. Abubakar, Yassir | Sun, Jun | Wu, Huanmei | Lu, Jianxin | Ni, Ya | Zhang, Xiaoyan | Xu, Jianqing\nAIDS Research and Human Retroviruses 2013;29(1):191-197.\nThe main heroine traffic from Yunnan province to the Xinjiang Autonomous Region is believed to initiate the transmission of CRF07_BC which is the predominant strain in intravenous drug users (IDUs) in China. However, the great distances between Yunnan and Xinjiang lead to an unclear and elusive diffusion process of CRF07_BC due to the absence of an important middle site such as Sichuan province. Moreover, in recent years the rapidly increasing infection rate among IDUs in the Liangshan region of Sichuan made it necessary to characterize the genetic character of the circulating strain of Sichuan IDUs. In this study, we characterized the genetic character of seven newly isolated CRF07_BC genomes (five from Sichuan and two from Xinjiang) and analyzed the transmission linkage among strains from IDUs in different regions. By conducting Markov chain Monte Carlo (MCMC) analysis and reconstruction of neighbor-joining trees and maximum-likelihood trees, our results revealed the genetic variation and important role of Sichuan-derived CRF07_BC strains during the transmission of CRF07_BC.\n10. Multiple Independent Introductions of HIV-1 CRF01_AE Identified in China: What Are the Implications for Prevention? Abubakar, Yassir F. | Meng, Zhefeng | Zhang, Xiaoyan | Xu, Jianqing\nPLoS ONE 2013;8(11):e80487.\nCRF01_AE gag p17 sequences were retrieved from public databases to explore phylogenetic relationships and phylogeographic dynamics of CRF01_AE in Asia by using maximum-likelihood phylogenetics and Bayesian coalescent-based analyses. We found close phylogenetic relationships between sequences from Thailand, Vietnam and China. Moreover, at least 5 independent introductions and 5 independent autochthonous clades of CRF01_AE, which descended from Thailand or Vietnam were identified in China from 1991 through 2003.\nThe current study not only defines the migration of CRF01_AE clades to/in Asia, but also demonstrates the criticalness of identifying the circulating strains in the population for the development of vaccine and microbicides. doi:10.1371/journal.pone.0080487\n11. Identification of Dominant Antibody-Dependent Cell-Mediated Cytotoxicity Epitopes on the Hemagglutinin Antigen of Pandemic H1N1 Influenza Virus Srivastava, Vikram | Yang, Zheng | Hung, Ivan Fan Ngai | Xu, Jianqing | Zheng, Bojian | Zhang, Mei-Yun\nJournal of Virology 2013;87(10):5831-5840.\n12. Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus Qiu, Chao | Huang, Yang | Zhang, Anli | Tian, Di | Wan, Yanmin | Zhang, Xiaoling | Zhang, Wanju | Zhang, Zhiyong | Yuan, Zhenghong | Hu, Yunwen | Zhang, Xiaoyan | Xu, Jianqing\nEmerging Infectious Diseases 2013;19(10):1685-1687.\nInfluenza; subtype H7N9; serology; neutralization tests; pseudovirus; viruses\n13. Enzymatic Excision of Uracil Residues in Nucleosomes Depends on Local DNA Structure and Dynamics† Ye, Yu | Stahley, Mary R. | Xu, Jianqing | Friedman, Joshua I. | Sun, Yan | McKnight, Jeffrey N. | Gray, Jeffrey J. | Bowman, Gregory D. | Stivers, James T.\n14. Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates Li, Liangzhu | Ben, Yinyin | Yuan, Songhua | Liu, Aiping | Wu, Huanmei | Xu, Jianqing | Zhang, Xiaoyan\nEmerging Microbes & Infections 2013;2(7):e42-.\nTo explore early biomarkers for establishing more sensitive safety evaluation assays in preclinical settings that determine the potential risks during the application of microbicide candidates, three representative microbicide candidates (cellulose sulphate, nonoxynol-9 and tenofovir), whose safety profiles have been well established in clinical trials, were included to gauge the sensitivities of different assays. Both mouse models and cell lines were employed to determine the sensitivities. The recruitment of immune cells at topical mucosal sites and the upregulation of HIV receptor/coreceptors in vitro were identified as highly sensitive biomarkers of the impact of microbicide candidates. Our data suggest that different evaluations/assays have their inherent sensitivities, and at least one assay from each sensitivity level should be included in the safety evaluation algorithm.\nHIV; microbicides; coreceptor; recruitment; receptor; safety; sensitivity\n15. Serverification of Molecular Modeling Applications: The Rosetta Online Server That Includes Everyone (ROSIE) Lyskov, Sergey | Chou, Fang-Chieh | Conchúir, Shane Ó. | Der, Bryan S. | Drew, Kevin | Kuroda, Daisuke | Xu, Jianqing | Weitzner, Brian D. | Renfrew, P. Douglas | Sripakdeevong, Parin | Borgo, Benjamin | Havranek, James J. | Kuhlman, Brian | Kortemme, Tanja | Bonneau, Richard | Gray, Jeffrey J. | Das, Rhiju\n16. New Emerging Recombinant HIV-1 Strains and Close Transmission Linkage of HIV-1 Strains in the Chinese MSM Population Indicate a New Epidemic Risk Wu, Jianjun | Meng, Zhefeng | Xu, Jianqing | Lei, Yanhua | Jin, Lin | Zhong, Ping | Han, Renzhi | Su, Bin\nPLoS ONE 2013;8(1):e54322.\nIn recent years, the population of men who have sex with men (MSM) have become the most significant increasing group of HIV-1 transmission in China. To identify new recombinant strains and transmission patterns of HIV-1 in Chinese MSM population, a cross-sectional investigation of MSM in Anhui Province (in south-eastern China) was performed in 2011. The diagnosed AIDS case rate, CD4 T-cell counts, HIV subtypes, and origin of the recombinant strains were investigated in 138 collected samples. The phylogenetic and bootscan analyses demonstrated that, apart from three previously reported circulating strains (CRF07_BC, CRF01_AE, subtype B), various recombinant strains among subtype B, subtype C, CRF01_AE, and CRF07_BC were simultaneously identified in Chinese MSM for the first time. The introducing time of B subtype in Chinese MSM populations was estimated in 1985, CRF01_AE in 2000, and CRF07_BC in 2003; the latter two account for more than 85% of MSM infections. Notably, in comparison with B subtype infections in Anhui MSM, CRF01_AE, with the highest prevalence rate, may accelerate AIDS progression. Over half of patients (56%) infected with new recombinant strains infection are diagnosed as progression into AIDS. Both Bayes and phylogenetic analyses indicated that there was active HIV transmission among MSM nationwide, which may facilitate the transmission of the new 01B recombinant strains in MSM. In conclusion, new recombinant strains and active transmission were identified in the Chinese MSM population, which may lead to a new alarming HIV pandemic in this population due to the increased pathogenesis of the newly emerging strains.\n17. A New Migration Map of HIV-1 CRF07_BC in China: Analysis of Sequences from 12 Provinces over a Decade Meng, Zhefeng | Xin, Ruolei | Zhong, Ping | Zhang, Chiyu | Abubakar, Yassir F. | Li, Jingyun | Liu, Wei | Zhang, Xiaoyan | Xu, Jianqing\nPLoS ONE 2012;7(12):e52373.\n18. The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine Wan, Yanmin | Wang, Jing | Zhou, Haizhu | Hu, Zhidong | Ren, Xiaonan | Xu, Jianqing\nClinical and Developmental Immunology 2012;2012:478052.\nPrevious studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8+ T cells. We found that frequencies of specific CD8+ T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8+ T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+ T cell receptor.\n19. Development of Skewed Functionality of HIV-1-Specific Cytotoxic CD8+ T Cells from Primary to Early Chronic Phase of HIV Infection Wang, Wanhai | Qiu, Chenli | Qiu, Chao | Wang, Ying | Zhang, Xiaoyan | Xu, Jianqing\nPLoS ONE 2012;7(9):e44983.\nIn recent years, the prevalence of HIV-1 infection has been rapidly increasing among men who have sex with men (MSM). However, it remains unknown how the host immune system responds to the infection in this population. We assessed the quantity of HIV-specific CD8+ T-cell responses by using Elispot assay and their functionalities by measuring 5 CD8+ T-cell evaluations (IL-2, MIP-1β, CD107a, TNF-α, IFN-γ) with flow cytometry assays among 18 primarily and 37 early chronically HIV-infected MSM. Our results demonstrated that subjects at early chronic phase developed HIV-specific CD8+ T-cell responses with higher magnitudes and more diversified functionalities in comparison with those at primary infection. However, populations with IL-2+ CD107a+ or in combination with other functionality failed to develop in parallel. The multifunctional but not monofunctional HIV-specific CD8+ T cells were associated with higher CD4+ T -cell counts and lower viral loads. These data revealed that prolonged infection from primary to early chronic infection could selectively increase the functionalities of HIV-specific CD8+ T cells in HIV-infected MSM population, the failure to develop IL-2 and cytotoxic functionalities in parallel may explain why the increased HIV-specific CD8+ T cells were unable to enhance the containment of HIV-1 replication at the early chronic stage.\n20. Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates Li, Liangzhu | Ben, Yinyin | Zhu, Zhaoqin | Li, Weihua | Xu, Jianqing | Zhang, Xiaoyan\nPLoS ONE 2012;7(8):e43211.\n21. Efficacy, Stability, and Biosafety of Sifuvirtide Gel as a Microbicide Candidate against HIV-1 Li, Liangzhu | Ben, Yinyin | Yuan, Songhua | Jiang, Shibo | Xu, Jianqing | Zhang, Xiaoyan\nPLoS ONE 2012;7(5):e37381.\n22. Boosting Heterosubtypic Neutralization Antibodies in Recipients of 2009 Pandemic H1N1 Influenza Vaccine Qiu, Chao | Huang, Yang | Wang, Qian | Tian, Di | Zhang, Wanju | Hu, Yunwen | Yuan, Zhenghong | Zhang, Xiaoyan | Xu, Jianqing\nClinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America 2011;54(1):17-24.\nOur data demonstrated that the inoculation with vaccine derived from the 2009 pandemic influenza raised vigorous neutralization antibodies against both cognate H1N1 and heterotypic influenza viruses. This observation has important implication for vaccine development.\nBackground. A mass vaccination has been implemented to prevent the spread of 2009 pandemic influenza virus in China. Highly limited information is available on whether this vaccine induces cross-reactive neutralization antibodies against other subtypes of influenza viruses.\nMethods. We employed pseudovirus-based assays to analyze heterosubtypic neutralization responses in serum samples of 23 recipients of 2009 pandemic influenza vaccine.\nResults. One dose of pandemic vaccine not only stimulated good neutralization antibodies against cognate influenza virus 2009 influenza A (H1N1), but also raised broad cross-reactive neutralization activities against seasonal H3N2 and highly pathogenic avian influenza virus H5N1 and lesser to H2N2. The cross-reactive neutralization activities were completely abolished after the removal of immunoglobin G (IgG). In contrast, H1N1 vaccination alone in influenza-naive mice elicited only vigorous homologous neutralizing activities but not cross-reactive neutralization activities.\nConclusions. Our data suggest that the cross-reactive neutralization epitopes do exist in this vaccine and could elicit significant cross-reactive neutralizing IgG antibodies in the presence of preexisting responses. The exposure to H1N1 vaccine is likely to modify the hierarchical order of preexisting immune responses to influenza viruses. These findings provide insights into the evolution of human immunity to influenza viruses after experiencing multiple influenza virus infections and vaccinations.\n23. Early Adaptive Humoral Immune Responses and Virus Clearance in Humans Recently Infected with Pandemic 2009 H1N1 Influenza Virus Qiu, Chao | Tian, Di | Wan, Yanmin | Zhang, Wanju | Qiu, Chenli | Zhu, Zhaoqin | Ye, Ruiqi | Song, Zhigang | Zhou, Mingzhe | Yuan, Songhua | Shi, Bisheng | Wu, Min | Liu, Yi | Gu, Shimin | Wei, Jun | Zhou, Zhitong | Zhang, Xiaoyan | Zhang, Zhiyong | Hu, Yunwen | Yuan, Zhenghong | Xu, Jianqing\nPLoS ONE 2011;6(8):e22603.\n24. HCV Coinfection Associated with Slower Disease Progression in HIV-Infected Former Plasma Donors Naïve to ART Zhang, Xiaoyan | Xu, Jianqing | Peng, Hong | Ma, Yan | Han, Lifeng | Ruan, Yuhua | Su, Bing | Wang, Ning | Shao, Yiming\nPLoS ONE 2008;3(12):e3992.\nIt remains controversial how HCV coinfection influences the disease progression during HIV-1 infection. This study aims to define the influence of HCV infection on the replication of HIV-1 and the disease progression in HIV-infected former plasma donors (FPDs) naïve to ART.\n168 HIV-1-infected FPDs were enrolled into a cohort study from Anhui province in central China, and thereafter monitored at month 3, 9, 15, 21 and 33. Fresh whole blood samples were used for CD4+ T-cell counting. Their plasma samples were collected and stored for quantification of HIV-1 viral loads and for determination of HCV and Toxoplasma. Out of 168 HIV-infected FBDs, 11.9% (20 cases), 80.4% (135 cases) and 7.7% (13 cases) were infected with HIV-1 alone, HIV-1/HCV and HIV/HCV/Toxoplasma, respectively. During the 33-month follow-up, only 35% (7 out of 20 cases) HIV-1 mono-infected subjects remained their CD4+ T-cell counts above 200 cells/µl and retained on the cohort study, which was significantly lower than 56% (75 out of 135 cases) for HIV/HCV group and 69% (9 out of 13 cases) for HIV/HCV/Toxoplasma group (p<0.05). CD4+ T cells in HIV mono infection group were consistently lower than that in HIV/HCV group (p = 0.04, 0.18, 0.03 and 0.04 for baseline, month 9, month 21 and month 33 visit, respectively). In accordance with those observations, HIV viral loads in HIV mono-infection group were consistently higher than that in HIV/HCV group though statistical significances were only reached at baseline (p = 0.04).\nThese data indicated HCV coinfection with HIV-1 is associated with the slower disease progression at the very late stage when comparing with HIV-1 mono-infection. The coinfection of Toxoplasma with HIV and HCV did not exert additional influence on the disease progression. It will be highly interesting to further explore the underlying mechanism for this observation in the future.\n25. Comparison of Immunogenicity between Codon Optimized HIV-1 Thailand Subtype B gp140 and gp145 Vaccines Wan, Yanmin | Wu, Lan | Liu, Lianxing | Xu, Jianqing | Liu, Ying | Liu, Yong | Shao, Yiming\nHIV-1 pandemic posed an unprecedent challenge to the global health and it is believed that an effective vaccine will be the final solution against HIV-1. HIV-1 envelope is the primary immunogen in developing neutralization antibody based HIV vaccine. To define the suitable Env derived immunogen, we systemically compared the immunogenicity of gp140 and gp145 in a DNA vaccination alone and a prime-boost modalities. 2 DNA vaccines and 2 recombinant Tiantan vaccinia vaccines (rTTV) were constructed for vaccination of female Balb/c mice. Elispot assay was used to read out the T cell immunity and ELISA assay was used to quantify antibody immunity. PLL (poly-L-Leucine)-ELISA assay was used in linear antibody epitope mapping. Mice primed with gp145 tended to elicit more Env-specific T cells responses than those primed with gp140, significant difference was observed in DNA immunization alone. The ultimate T cell responses in prime-boost regimen tends to be determined mainly by the priming efficacy. Linear antibody epitope mapping displayed that sera raised by gp145 priming were vigorously reactive to more peptides than that by gp140. Our data demonstrated HIV-1 Thailand B-derived gp145 may raise higher T-cell responses and broader linear peptide-specific antibody responses than gp140 does. However, it remains to be determined that how these observations are relevant to neutralization antibody activities.\nHIV-1; Vaccine; Envelope; Immunogen design


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url: =Chengdu-Lhasa-Mt.E.B.C-Zhangmu 16-day land cruise-China Tours from CITS\nChengdu-Lhasa-Mt.E.B.C-Zhangmu 16-day land cruiseTourCode:HT-36Destination:Chengdu-Leshan-Southern Sichuan-Tibet HWY-Lhasa-Shigatse-Mt.Everest-ZhangmuAttraction:Panda,Leshan Giant Buddha,Sichuan-Tibet Southern Route,Jokhang Temple,Barkor Market Street,Potola Palace,Tibet Museum,Norbulink Palace,Drepung Monastery,Yamdrok,Pelkor Monastery,Sakya Monastery,Rongbuk,ZhangmuPrice: USDDepartureDate:Want to travel from china inner land to Tibet?Want to experience Lhasa-Kathmandu Friendship Hwy? Both of your thoughts can be done in one tour.\nChengdu-Lhasa-Mt.E.B.C-Zhangmu 16-day land cruise Tour DatesDestinationsToday's Activities Day 1Arrive in ChengduArrive in ChengduDetailed Day 2Chengdu-Panda-Leshan Giant BuddhaPanda Base and Leshan Giant BuddhaDetailed Day 3Chengdu-Ya'an-Luding-XinduqiaoDrive to XinduqiaoDetailed Day 4Xinduqiao-Yajiang-Litang-BatangDrive along Xinduqiao-Yajiang-Litang-BatangDetailed Day 5Batang-MangkangDrive along Batang-MangkangDetailed Day 6Mangkang-Zuogong-BasuDrive along Mangkang-Zuogong-BasuDetailed Day 7Basu-Rawok-BomeDrive along Basu-Rawok-BomeDetailed Day 8Bome-Tongmai-BayiDrive along Bome-Tongmai-BayiDetailed Day 9Nyingchi-Druksom lake-LhasaDrive along Nyingchi-Basong lake-LhasaDetailed Day 10Sight-seeing in Lhasa cityLhasa city one day tour, visit Jokhang Temple, Barkor Market Street and Potola PalaceDetailed Day 11Sight-seeing in Lhasa cityLhasa city one day tourDetailed Day 12Lhasa- Gyantse-ShigatseYamdrok, Pelkor MonasteryDetailed Day 13Shigatse- ShegarTashilunpo Monastery and Sakya MonasteryDetailed Day 14Shegar -Rongbuk-E.B.CRongbuk and Mt.Everest Base CampDetailed Day 15Rongbuk-ZhangmuArrive at Zhangmu and end the tourDetailed Day 16Zhangmu exitend the tourDetailedPrice details of small group tour\nabove 8 persons\nOverviewChengdu-Lhasa-Mt.E.B.C-Zhangmu 16-day land cruiseDiscovery PlusPrice Includes and Excludes


url: =Yi Gan Fu Zheng Jiao Nang -For Hepatitis(Deficiency of Liver&Kid [HSTBDGY-2] - $22.00 : HESHOUTANG TCM HEALTH MALL, HERBAL PRODUCTS WORLD\nTongrentang Ruhe Neixiao Fluid-For Breast Hyperplasia\nHome :: Herbs Medicine :: Hepatitis :: Yi Gan Fu Zheng Jiao Nang -For Hepatitis(Deficiency of Liver&Kid\nYi Gan Fu Zheng Jiao Nang -For Hepatitis(Deficiency of Liver&Kid\nYi Gan Fu Zheng Jiao Nang -For Hepatitis(Deficiency of Liver&Kidney)\n[Product Name] Yi Gan Fu Zheng Jiao Nang/Yi Gan Fu Zheng Capsule\n[Ingredients] He Shou Wu, Hu Zhang, Guan Zhong, Rou Gui, Ming Fan, Dang Gui, Dan Shen, Ren Shen, Ma Huang.\n[Functions] Bu Gan Shen, Yi Qi Huo Xue. It is effective for HBV(Hepatitis B) due to Deficiency of Liver&Kidney).Typical Symptoms:dull pain in the hypochondrium, fatigue, soreness and weakness in the loins and knees, shortness of breath, palpitations, spontaneous sweating,dizziness,etc. (To tell which kind of Viral Hepatitis you have got HERE)\n2. Not take this product for Damp-heat Retention, Liver Qi Stagnation and Blood Stasis. (To tell which kind of cold you have got here.\n3.Patients with diabetes,kidney disease and other severe chronic diseases shall take medicine under guidance of physician.\nYi Gan Fu Zheng Jiao Nang is recommended as family normal medicine for HBV (Hepatitis B) due to Deficiency of Liver&Kidney.


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url: =食品胶体与软物质团队（Laboratory of Food Colloids and Soft Materials）\nNan Yang, Younan Feng, Chunxia Su, Qian Wang, Yuemei Zhang, Yanhong Wei, Meng Zhao, Katsuyoshi Nishinari, Yapeng Fang, Structure and tribology of κ-carrageenan gels filled with natural oil bodies, Food Hydrocolloids 2020, 105945. (IF: 6.103)\nNan Yang, Chunxia Su, Yuemei Zhang, Junji Jia, Robert L. Leheny, Katsuyoshi Nishinari, Yapeng Fang, G. O. Phillips, In situ nanomechanical properties of natural oil bodies studied using atomic force microscopy, J. Colloid &Inter. Sci.2020, 570, 362-374. (IF: 6.361)\nYao Xu#, Nan Yang#, Jinxin Yang, Jing Hu, Ke Zhang, Katsuyoshi Nishinari, Yapeng Fang, Glyn. O. Phillips, Protein/Polysaccharide Intramolecular Electrostatic Complex as Superior Food-Grade Foaming Agent, Food Hydrocolloids 2020, 101, 105474. (IF: 6.103)\nNan Yang, Ruihe Lv, Junji Jia, Katsuyoshi Nishinari, Yapeng Fang*, Application of microrheology in food science, Annu. Rev. Food Sci.& Tech. 8(23), 493-521 (2017). (IF: 8.511)\nMeng Zhao, Xue Huang, Hui Zhang, Yanzhen Zhang, Michael Gänzle, Nan Yang, Katsuyoshi Nishinari, Yapeng Fang, Probiotic encapsulation in water-in-water emulsion via heteroprotein complex coacervation of type-A gelatin/sodium caseinate, Food Hydrocolloids 2020, 105, 105790. (IF: 6.103)\nBing Hu#; Lingyu Han#; Ruixiang Ma; Glyn Owen Phillips; Katsuyoshi Nishinari; Yapeng Fang; All-natural food-grade hydrophilic-hydrophobic core-shell microparticles: facile fabrication based on gel-network-restricted antisolvent method, ACS Applied Materials & Interfaces, 2019, 11: 11936-11946. (IF: 8.694)\nBing Hu; Lingyu Han; Huiling Kong; Katsuyoshi Nishinari; Glyn Owen Phillips; Jixin Yang; Yapeng Fang; Preparation and emulsifying properties of trace elements fortified gum arabic, Food Hydrocolloids, 2019, 88: 43-49. (IF: 6.103)\nYuemei Zhang, Nan Yang*, Yao Xu, Qian Wang, Ping Huang, Katsuyoshi Nishinari, Yapeng Fang, Improvement of stability of oil body emulsions from diverse plant seeds by sodium alginate, Molecules 2019, 24, 3856. (IF: 3.060)\nLingyu Han#; Bing Hu#; Ruixiang Ma; Zhiming Gao; Katsuyoshi Nishinari; Glyn Owen Phillips; Jixin Yang; Yapeng Fang; Effect of arabinogalactan protein complex content on emulsification performance of gum arabic, Carbohydrate Polymer, 2019, 224, 115170. (IF: 6.044)\nKatsuyoshi Nishinari, Mihaela Turcanu, Makoto Nakauma, Yapeng Fang, Role of fluid cohesiveness in safe swallowing, Npj Sci. Food. 2019, 3, 5.\nKatsuyoshi Nishinari, Yapeng Fang, Andrew Rosenthal, Human oral processing and texture profile analysis parameters: Bridging the gap between the sensory evaluation and the instrumental measurements. J. Texture Stud., 2019, 369-380. (IF: 1.565)\nChunxia Su, Younan Feng, Jing Ye, Zhiming Gao, Meng Zhao, Nan Yang*, Katsuyoshi Nishinari, Y. Fang, Effect of sodium alginate on the stability of soybean natural oil body emulsions, RSC Advances, 8, 4731- 4741 (2018). (IF: 3.049)\nMeng Zhao, Ying Wang, Xue Huang, Michael Gaenzle, Zhengjun Wu, Katsuyoshi Nishinari, Nan Yang, Yapeng Fang, Ambient storage of microencapsulated Lactobacillus plantarum ST-III by complex coacervation of type-A gelatin and gum Arabic, Food & Function, 2018, 9(2), 1000-1008. (IF: 3.241)\nBing Hu; Lingyu Han; Zhiming Gao; Ke Zhang; Saphwan Al-Assaf; Katsuyoshi Nishinari; Glyn Owen Phillips; Jixin Yang; Yapeng Fang*; Effects of Temperature and Solvent Condition on Phase separation induced molecular fractionation of gum arabic/hyaluronan aqueous mixtures, International Journal of Biological Macromolecules, 2018, 116: 683-690. (IF: 4.784)\n叶晶，杨楠*，李静，张岳梅，黄萍，王倩，高志明，Katsuyoshi Nishinari, 方亚鹏, 利用示踪粒子及膨胀流变学研究β-乳球蛋白聚集体的界面行为,食品科学，2019.11.15.\n叶晶，吕瑞鹤，苏春霞，冯有楠，李静，杨楠*，方亚鹏，大豆分离蛋白溶液/乳液酸化过程的微观流变学研究, 中国食品学报 2019，3，23-33.\nNan Yang, Chunxia Su, Yapeng Fang, Enhancement of the dispersability of natural oil bodies using alginate and their application in salad dressing, Application No. 201810082759.7\n联系人： 杨楠 tyc娱乐网站轻工楼A423


url: =Hikvision Holds Shaping Intelligence AI Cloud World Summit - News - Yiwu Hongyuan Electronic Technology Co.,Ltd\nPrevious: A Video Recorder Can Connect Two Monitors\nNext: Hikvision - Artificial Intelligence Technology\nCan Non-POE Devices Connect To POE Switches?\nWhat Is Smart264/smart 265?\nHow Does The Hikvision VCR Link With A Wireless...\nThe Role Of Wide-angle Lens\nWide Dynamic Features


url: =wrong timing! (Page 2) | 3422174 | Qubool Hai Forum\nwrong timing! (Page 2)


url: =Shenzhen jiawaijia chain hotel, xem đánh giá và giá phòng | Trip.com\nTrang chủKhách sạnKhách sạn ở Thâm QuyếnShenzhen jiawaijia chain hotel\nShenzhen jiawaijia chain hotel\nNo.45 Baogang Road (Chaohui Building), Thâm Quyến, 518000, Quảng Đông, Trung QuốcXem trên bản đồ\nKhai trương: 2014 The Shenzhen jiawaijia chain hotel is an ideal spot for travelers wanting to discover the city. Visitors to Shenzhen will find that the Shenzhen jiawaijia chain hotel is a fantastic accommodation choice.Xem thêm\nĐánh giá(163 đánh giá xác thực của khách)\nOK, môi trường ở mức trung bình\nỞ đây lâu, rất tốt\nỞ lâu, chủ khách sạn và nhân viên rất dễ mến.\nXấu, ồn ào, hơi bẩn\n[Thích hợp cho trẻ em, rất gần ga tàu điện ngầm, bên ngoài là phố đi bộ, vị trí rất tốt, chi phí cao, dịch vụ tốt]\nThe Shenzhen jiawaijia chain hotel is an ideal spot for travelers wanting to discover the city. Visitors to Shenzhen will find that the Shenzhen jiawaijia chain hotel is a fantastic accommodation choice. Traveling to the hotel is easy with Shenzhen Railway Station located approximately 6km away and Shenzhen Bao'an International Airport roughly 40km away. Just a short walk from Sungang Metro Station, traveling to most city destinations is a breeze. There's plenty to do nearby, with Honghushequ Square, Honghu Huayuan Hujing Shequ Wenti Square and Datang Long Shequ Wenti Park all within a short distance. When guests have some time on their hands they can make use of the onsite facilities. This Shenzhen hotel provides parking on site. Perfect for those working on the move, this hotel caters to a large number of business guests.\nWi-Fi ở khu vực công cộngMiễn phíQuầy lễ tân (24 giờ)Gọi đánh thứcKho hành lýMiễn phíKhu vực hút thuốc\nCách khách sạn khoảng (50 phút) khi đi ô tô (39,5 km)\nQiyou Capsule Youth Hostel | Jinghuang Theme Apartment | Jinjiang Inn (Shenzhen Futian Exhibition Center Fumin Metro Station) | Vienna International Hotel (Shenzhen Songgang Hongxing) | Sanyuehai Youth Inn | Qicheng Premium Hotel (Shenzhen Hongfa Dashijie Store) | Shuge Apartment Hotel | Little Hakka Hotel | Shenzhen Naisihao Inn | Xinhu Theme Hotel | Lavande Hotel (Shenzhen North Railway Station Longhua Bus Terminal) | Cjia Aparthotel (Shenzhen Nanshan Science and Technology Park) | Kailun Boutique Hostel | Langrong Business Hostel | Huali Hotel | Yuchang Business Hotel | Argyle Hotel Shenzhen Nanshan By Aria | Halcyon Inn | Yunxuan Inn | Houpu Short-term Rental Apartment (Shenzhen Nanshan Nanyou Xinyidai)


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=Ji mao fei shang tian : Feather flies to the sky (Work) - Randwick City Library\nThe work Ji mao fei shang tian : Feather flies to the sky represents a distinct intellectual or artistic creation found in Randwick City Library. This resource is a combination of several types including: Work, Moving Image, Visual Materials.\nThe Resource Ji mao fei shang tian : Feather flies to the sky\nHangzhou jia ping ying ye you xian gong si chu pin\nHangzhou jia ping ying ye you xian gong si\nDirector, Yu Ding ; screenwriter, Shen Jie\nZhang Yi, Yin Tao, Tao Zeru, Zhang Jianing, Gao Zhuyao, Hua Kun, Lin Yiting\nContext of Ji mao fei shang tian : Feather flies to the sky\nJi mao fei shang tian : Feather flies to the sky, Hangzhou jia ping ying ye you xian gong si chu pin, (videorecording)\n
 Data from Ji mao fei shang tian : Feather flies to the sky - Randwick City Library
\nData Citation of the Work Ji mao fei shang tian : Feather flies to the sky