url: =Studies on the Screening Index for Low Nitrogen Tolerant Rice and Its Selection--《Journal of Anhui Agricultural Sciences》2009年16期\nStudies on the Screening Index for Low Nitrogen Tolerant Rice and Its Selection\nZHENG Jia-kui et al(College of Bioenginreering,Chongqing University,Chongqing 400044)\n[Objective] The purpose of this research was to ascertain the screening index for low nitrogen tolerant rice and its selection.[Method]A field trial was carried out to evaluate 106 rice lines under N stress and normal N supply condition for plant height,panicle length,panicle number,biomass yield,single yield,1 000-grain weight and seed set in mature stage.[Result]The results showed that relative panicle number,relative biomass yield and relative single yield(low N supply /normal N supply) were sensitive to N stress and demonstrated significant variations in different rice lines(the CV were 21.97%、21.29% and 22.13%,respectively).Correlation analysis showed that relative plant height,relative seed set were significantly or highly significantly correlated to RPN,RBY and RSY.Therefore,plant height,panicle number,biomass yield,single yield and seed set in mature stage would probably be effective screening indexes for screening low nitrogen tolerant rice.[Conclusion]This study will provide the theoretical references for screening index for low nitrogen tolerant rice and its selection and culturing new variety.\n【Fund】： 四川省“十一五”攻关项目;; 四川省农业科学院青年基金项目\n1 LU Zhi-zhong et al(Hubei Provincial Seed Group CO.,LTD,Wuhan,Hubei 430070);Selection Experiment of Chinese Hybrid Rice in Pakistan[J];Journal of Anhui Agricultural Sciences;2012-07\n2 Zhou Degui 1 Li Hong 1 Lu Decheng 1 Huang Daoqiang 1 Lai Suichun 1 Wang Zhidong 1 Zhou Shaochuan 1,2 1 The Rice Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou,510640;2 College of Agricultural Science,Hu'nan Agricultural University,Changsha,410128;The Exploration and Establishment of Rice Breeding Materials of High Phosphorus Using Efficiency and Nitrogen Using Efficiency[J];Molecular Plant Breeding;2010-06\n3 ZHANG Ning;GUO Rong-fa;Faculty of Agricultural Science,Guangdong Ocean Univisity;;Advancements in nitrogen efficient screening of germplasm resources and deficiency tolerance mechanisma study in rice[J];Guangdong Agricultural Sciences;2014-05\n4 WEI Mao-gui,WANG Xiao-yu,XIE Guang-hui*(College of Agronomy and Biotechnology/National Energy R&D Center for Non-food Biomass, China Agricultural University,Beijing 100193,China);Maturity date of main field crop residues in different regions of China[J];Journal of China Agricultural University;2012-06\n5 YAN Ming-jian,HUANG Wen-zhang,HU Jing-tao,L Zhi-wen,LEI Shu-fan,HUANG Cheng-zhi(The Chongqing Three Gorges Academy of Agricultural Sciences,Chongqing 404155,China);Cluster Analysis of Nitrogen Efficiency of Rice Genotypes[J];Hubei Agricultural Sciences;2012-21\n6 FAN Shu-xiu;XU Zheng-jin;YANG Qing-chuan;Rice Research Institute of Shenyang Agricultural University / Key Laboratory of Northeast Rice Biology , Genetics and Breeding of Ministry of Agriculture / Key Laboratory of Northern Japonica Rice Breeding of Liaoning;;Classification and Evaluation on Different Rice Genotypes with Nitrogen Use Efficiency[J];Hubei Agricultural Sciences;2014-01\n7 Zhou Tong1, Wang Xiaochun2, Wang Yan2, Zhang Shuhua1, Wang Guiqiang2, Zhou Jianchao2 (1Jiamusi Rice Institute of the Academy of Heilongjiang Agricultural Science, Jiamusi Heilongjiang 154026; 2Heilongjiang University, Harbin 150080);Screening of Different Japonica Rice Germplasm Tolerance to Nitrogen Deficiency in Cold Region of China[J];Chinese Agricultural Science Bulletin;2011-33\n8 LI Mao-mao1,WAN Jian-lin1,HUANG Yong-lan1,CAO Gui-lan2,CHEN Hong-ping1,HAN Long-zhi2 (1Rice Research institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200;2Key Laboratory of Crop Germplasm Resources and Biotechnology,Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement,NFCRI/Insitute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081);Evaluation and Correlation Analysis of the Related Characters of Nitrogen Use Efficiency for Mini Core Collection of Rice(Oryza sativa L.)in China[J];Journal of Plant Genetic Resources;2011-03\n9 YANG Qing-chuan,XU Zheng-jin,FAN Shu-xiu;Screening for Low Nitrogen Tolerance Genotypes at Rice Seedling Stage in Three Provinces of the Country’s Northeast[J];Seed;2012-10\n10 Wang Chao;Xiang Chao;Qu Lijun;Zhang Junwei;Shi Yingyao;School of Agronomy,Agricultural University of Anhui;Institute of Crop Science,Chinese Academy of Agricultural Sciences;;The Advance of Physiological Mechanism of Nitrogen Assimilation-Transport and Utilization,and Genetic Basis of Low Nitrogen Tolerance in Rice[J];Chinese Agricultural Science Bulletin;2014-03\n3 CUI Shi-you~(1),MIAO Ya-mei~(1),SHI Chuan-huai~(2),YU Cong-hua~(3) (1.Jiangsu Yanjiang Institute of Agricultural Science,Rugao Jiangsu 226541,China;2.Agri-tech Extension Center of Haian County,Haian Jiangsu 226600,China;3.Grain and Cotton Foundation Seed Nursery of Nantong City,Rugao Jiangsu 226541,China);Research and Prospects of Breeding for Nitrogen Efficiency in Rice[J];Review of China Agricultural Science and Technology;2006-06\n4 Cao Liming 1,2) Pan Xiaohua 1) ( 1) College of Agronomy, Jiangxi Agricultural University, Nanchang 330045; 2)Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106);ANALYSIS OF SOME INDEXES USED FOR EVALUATING TOLERANCE OF DIFFERENT RICE GENOTYPES TO LOW PHOSPHORUS TREATMENT IN SAND CULTURE[J];Acta Agriculturae Shanghai;2000-04\n5 WANG Xiu qing1, ZHANG Hong cheng1, HUANG Yin zhong1, DAI Qi gen1, HUO Zhong yang1, XU Ke1, SHAO Shi zhi1,XU Xun jun2 (1.College of Agriculture, Yangzhou University, Yangzhou 225009; 2.Bureau of Agriculture,Zhangjiagang City,Zhangjiagang 215600,China);Effects of N-Application Rate on the Characters of Uptake Nitrogen and Nitrogen Recovery of Different Rice Varieties[J];Journal of Shanghai Jiaotong University (Agricultural Science);2003-04\n6 Yang Xiaoe Sun Xi (Plant Nutri. 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3School of Plant Science, College of Agriculture & Life Sciences, Seoul National University, Sowoen 441-744, Korea);Variations of Nitrogen Use Efficiency by Rice Genotype[J];Chinese Journal of Rice Science;2003-03\n10 WU Ring; TAO Qinnan(Soil Science and Plant Nutrition Department, Zhejiang Agricultural University, Hangzhou 310029);Selection Index for Screening Physiological Nitrogen Use Efficiency in Rice[J];CHINESE JOURNAL OF RICE SCIENCE;1995-03\n2 Yu Lixiang Bian po Wu Yuejin et al.(Institute of Plasma Physics,Chinese Academy of Science,Anhui Hefei 230031);Influence of "Lose-control" of Fertilizer on Nitrogen Agronomic Utilized Coeffciency of Crop[J];Anhui Agricultural Science Bulletin;2007-24\n3 Ruan Xinmin Shi Fuzhi Luo Zhixiang Su Zesheng Ni Jinlong (Rice Research Institute,Key Laboratory of Rice Genetics and Breeding of Anhui Province,Anhui Academy of Agricultural Science;Anhui Hefei;230031);Effects of Different Types and Treatments of Fertilizer on Agronomic N Utilized Coefficiency of Various Rice Cultivars[J];Anhui Agricultural Science Bulletin;2007-24\n5 CHEN Zhou-qian et al (Rice Reseach lnstitute.AAAS,Hefei,Anhui 230031);Study on the Technique of N-fertilizer Application[J];Journal of Anhui Agricultural Sciences;2005-05\n6 RUAN Xin-min et al (Rice Research Institute, Anhui Academy of Agriculture Sciences, Hefei,Anhui 230031 );Effects of Nitrogen-fertilization on the Agronomic Use Efficiency of the Different Rice Varieties[J];Journal of Anhui Agricultural Sciences;2005-06\n7 FEI Su-e et al(College of Horticultural Science and Engineer,Shandong Agricultural University,Taian,Shandong 271018);Effects of Different Ratio and Amount of Nitrogen and Phosphorus in Nursing Media on Growth and Quality of Sweet Pepper Plug Seedling[J];Journal of Anhui Agricultural Sciences;2006-04\n8 SU Ze-sheng et al (Rice Research Institute of AAAS; Key Laboratory of Rice Genetics and Breeding Anhui Province; Hefei Branch of National Rice Improvement Center, Hefei, Anhui 230031);Physiology Characteristic and Agriculture Measure of Hybrid Rice Combination-Xieyou 9019 with High-yielding and Good-quality Anti-resistance[J];Journal of Anhui Agricultural Sciences;2006-08\n9 LIU Hui et al(College of Resources and Environment,Huazhong Agricultural University,Wuhan,Hubei 430070);Research Progress in the Selecting Standard of the Plant Genotype with Nitrogen Efficiency[J];Journal of Anhui Agricultural Sciences;2006-14\n10 YANG Jian-feng et al(College of Resource and Environment,Middle China Agricultural University,Wuhan,Hubei 430070);Research Advance in the Exudation of Organic Acid in Phosphorus-deficient Plant[J];Journal of Anhui Agricultural Sciences;2006-20\n4 ;Effects of Nitrogen and Phosphorus on the Root Physiology and Nutrient Uptake during Grain filling and Their Relations with Grain Quality of Rice[A];[C];2007\n5 ZHOU Guo-hua, ZENG Ya-wen, DU Juan, YANG Shu-ming, PU Xiao-ying, LIU Kun, LI Ben-xun, MU Yuan-li, LI Jia-fang, LING Qing (Biotechnology and Crop Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Yunnan Kunming 650205, China College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan Kunming 650201, China; Yunnan Agriculture Occupation Technical College, Yunnan Kunming 650031, China);Effect of low-nitrogen stress on heading date of the core collection for Yunnan rice landrace under low phosphorus condition[A];[C];2006\n7 Chen Yue-jun,Li Ting-mei,Du Zhong,Zhu Jing-jian (Pingchang soil and Fertilizer Station,,Pingchang 636400 China);Variations in phosphorus use efficiency by different wheat cultivars[A];[C];2012\n8 ZHANG Le~1 YANG Xiu-zhen~1 LIU Ke-feng~2 SHAO Wei-wei~2 (1 College of Landscape Architecture,Beijing Forestry University,Beijing 100083; 2 College of Landscape Department,Beijing University of Agriculture,Beijing 102206);Effects of Ammonium Nitrogenon and Nitrate Nitrogen on Growth Developmenth of Soilless Culture of Salvia splendens Ker.-Gawl[A];[C];2012\n2 Tai Dewei et al (Rice Research Institute, AAAS, Hefei 230031);Evaluation and Screen of the Core Germplasm of Integrated International Molecular Breeding Program for Low-phosphorous Tolerance in Seedling[J];Journal of Anhui Agricultural Sciences;2003-06\n5 ZHANG Jun-ying et al (College of Resources and Environment,Hebei University of Sciences,Tangshan,Hebei 063009);Study on Screening Maize Genotypes with High N Efficiency under N Stress[J];Journal of Anhui Agricultural Sciences;2007-22\n6 DING Hong et al (Department of Environment and Planning,Shangqiu Normal University,Shangqiu,Henan 476000);Effects of New Special Fertilizer on the Nodulation,Yield and Nutrient Utilization Efficiency of Peanut[J];Journal of Anhui Agricultural Sciences;2008-07\n8 ZHANG Qiang et al (Rice research Institute,Jilin Academy of Agricultural Science,Gongzhuling,Jilin 136100);Situation of the Introduce and Utilization on foreign Rice Germplasm in Jilin Province[J];Journal of Anhui Agricultural Sciences;2008-21\n10 WANG Feng-mei (College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193);Introduction and Utilization Situations of Worldwise Rice Germplasm Resources in China[J];Journal of Anhui Agricultural Sciences;2009-25\n1 Yi Junliang 1,2* Zhou Degui 2* Lu Decheng 2 Wu Yaorong 3 Li Hong 2 Huang Daoqiang 2 Lai Suichun 2 Wang Chongrong 2 Wang Zhidong 2 Chen Liyun 1 Xie Qi 3** Zhou Shaochuan 1,2** 1 Rice Research Institute,Hunan Agricultural University,Changsha,410128;2 Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640;3 Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101;Molecular Mapping of Phosphorus-Efficient Gene in Rice[J];Molecular Plant Breeding;2012-06\n2 YAO Guoxin;LU Lei;LI Changchun;HUANG Wenchao;School of Life Sciences Technology,Hubei Engineering University;College of Life Science,Wuhan University;;Detection of Restorer Gene Rf1aof BT Type in Cultivated Rice Mini-core Collection[J];Guizhou Agricultural Sciences;2014-04\n3 YAO Guo-xin;HU Jian;HUANG Ming-jun;DUAN Jun-zhi;Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetable;School of Life and Science Technology,Hubei Engineering University;Henan Academy of Agricultural Sciences;;Screening of Wide Compatibility Gene S5~n in Mini-core Germplasm of Cultivated Rice[J];Journal of Henan Agricultural Sciences;2014-11\n4 HUANG Yong-lan;LI Mao-mao;LU Ming;WAN Jian-lin;LONG Qi-zhang;WANG Hui-ming;TANG Xiu-ying;FAN Zhi-jie;Jiangxi Super-rice Research and Development Center;Rice Research Institute,Jiangxi Academy of Agricultural Sciences;;Selection of Rice Germplasm with High Nitrogen Utilization Efficiency and Its Analysis of the Related Characters[J];Journal of Plant Genetic Resources;2015-01\n1 Wang Ziqiang;Dong Mingyuan(Dept.Agron.,Zhejiang Agriculture University, Hangzhou, 310029);POTASSIUM USE EFFICIENCY OF SPRING SOYBEAN UNDER VARIOUS POTASSIUM SUPPLY[J];SOYBEAN SCIENCE;1996-03\n2 TONG Yi ping 1,LI Ji yun 1,LI Zhen sheng 2 (1.Research Center for Eco Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;2.Institute of Genetics,Chinese Academy of Sciences,Beijing 100101);Genotypic variations for nitrogen use efficiency in winter wheat I.Effects of N uptake and utilization efficiency on grain yields[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1999-02\n3 Luo Xinglu Tutor Tang Fuzhong (Department of Agronomy);STUDIES ON THE TECHNIQUE OF NITROGEN FERTILIZER APPLICATION TO LATE HYBRID RICE[J];;1989-03\n4 Yang Deguang et al. (PLA agric.and Anl.Husb.Univ.,Changchun 130062,China);Effect of N uptake characteristics and populational formation on the yield of dry seeded hybrid rice[J];HUBEI AGRICULTURAL SCIENCES;2000-04\n5 ZOU Chang ming,QIN Dao zhu,CHEN Fu xing,LIU Geng ling (Red Soil Experimental Station, CAAS, Qiyang, Hunan 426182,PRC);Technique of N Fertilizer Application on Rice Ⅰ. Appropriate Time and Rate of Application[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;2000-06\n6 Chen Caihong, Liu Chenliu( Huazhong Agricultural University, Wuhan );STUDIES ON THE NUTRITIONAL CHARACTERS OF NITROGEN OF HIGH YIELDING HYBRID RICE SHAN-YOU 63[J];Journal of Huazhong Agricultural;1989-01\n7 WT5,5”HZ]FENG Wei zhu XU Mao JI Chun mei SU Zu fang ZHOU Pei nan XU Nai xia ZHANG Ya jie ( 1. Agric. and Foresty Dept. of Jiangsu Province, Nanjing 210013, China; 2. Dept. of Agron., Agric. Coll., Yangzhou Univ., Yangzhou 225009, Chi;EFFECT OF NITROGEN FERTILIZER REGIME ON SOIL NUTRIENT SUPPLYING, YIELD AND ITS NITROGEN UPTAKING OF RICE PLANT[J];JIANGSU AGRICUTURAL RESEARCH;2000-03\n8 SHAN Yu hua 1 WANG Yu long 1 HUANG Jian ye 1 Ho Ando 2 Yamamoto Yoshinori 3 DONG Gui chun 1 ZHOU Xiao dong 1 ( 1. Dept. of Agron., Agric. Coll., Yangzhou Univ., Yangzhou 225009, China; 2. Faculty of Agric., Yamagata Univ., Tsuru;EFFECT OF NITROGEN FERTILIZER APPLIED AT MIDDLE AND LATE GROWING STAGES ON ~(15)NUPTAKE AND DISTRIBUTION IN RICE ( ORYZA SATIVA L.)[J];Jiangsu Agricutural Research;2000-04\n9 SHAN Yu hua 1, WANG Yu long 1, YAMAMOTO Yoshinori 2, HUANG Jian ye 1, DONG Gui chun 1, YANG Lian xin 1, ZHANG Chuan sheng 1, JU Jing 1 (1. Dept of Agron, Agric Coll, Yangzhou Univ, Yangzhou 225009, China; 2. 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url: =Referências encontradas : 2439 [refinar]\n1 / 2439 MEDLINE\n2 / 2439 MEDLINE\n[Au] Autor: Richardson SM; Mitchell LA; Stracquadanio G; Yang K; Dymond JS; DiCarlo JE; Lee D; Huang CL; Chandrasegaran S; Cai Y; Boeke JD; Bader JS\n[Ad] Endereço: Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.\n[Ti] Título: Design of a synthetic yeast genome.\n[So] Source: Science;355(6329):1040-1044, 2017 03 10.\n[Ab] Resumo: We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.\n[Mh] Termos MeSH secundário: Cromossomos Artificiais de Levedura/genética\n[do] DOI: 10.1126/science.aaf4557\n3 / 2439 MEDLINE\n[Au] Autor: Zahn LM; Riddihough G\n[Ti] Título: Building on nature's design.\n[So] Source: Science;355(6329):1038-1039, 2017 Mar 10.\n[Mh] Termos MeSH primário: Cromossomos Artificiais de Levedura\n[Mh] Termos MeSH secundário: Saccharomyces cerevisiae/crescimento & desenvolvimento\n[do] DOI: 10.1126/science.355.6329.1038\n4 / 2439 MEDLINE\n[Au] Autor: Kannan K; Gibson DG\n[Ad] Endereço: Synthetic Genomics, Inc., 11149 North Torrey Pines Road, La Jolla, CA 92037, USA.\n[Ti] Título: Yeast genome, by design.\n[So] Source: Science;355(6329):1024-1025, 2017 Mar 10.\n0 (Bacterial Proteins); EC 3.1.- (Cas9 protein, Francisella novicida); EC 3.1.- (Endonucleases)\n[do] DOI: 10.1126/science.aam9739\n5 / 2439 MEDLINE\n[Au] Autor: Shen Y; Wang Y; Chen T; Gao F; Gong J; Abramczyk D; Walker R; Zhao H; Chen S; Liu W; Luo Y; Müller CA; Paul-Dubois-Taine A; Alver B; Stracquadanio G; Mitchell LA; Luo Z; Fan Y; Zhou B; Wen B; Tan F; Wang Y; Zi J; Xie Z; Li B; Yang K; Richardson SM; Jiang H; French CE; Nieduszynski CA; Koszul R; Marston AL; Yuan Y; Wang J; Bader JS; Dai J; Boeke JD; Xu X; Cai Y; Yang H\n[Ti] Título: Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.\n[Ab] Resumo: Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by -mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.\n[Mh] Termos MeSH primário: Cromossomos Artificiais de Levedura/fisiologia\nCromossomos Artificiais de Levedura/química\n0 (Culture Media); PDC6A3C0OX (Glycerol)\n6 / 2439 MEDLINE\n7 / 2439 MEDLINE\n[Au] Autor: Xie ZX; Li BZ; Mitchell LA; Wu Y; Qi X; Jin Z; Jia B; Wang X; Zeng BX; Liu HM; Wu XL; Feng Q; Zhang WZ; Liu W; Ding MZ; Li X; Zhao GR; Qiao JJ; Cheng JS; Zhao M; Kuang Z; Wang X; Martin JA; Stracquadanio G; Yang K; Bai X; Zhao J; Hu ML; Lin QH; Zhang WQ; Shen MH; Chen S; Su W; Wang EX; Guo R; Zhai F; Guo XJ; Du HX; Zhu JQ; Song TQ; Dai JJ; Li FF; Jiang GZ; Han SL; Liu SY; Yu ZC; Yang XN; Chen K; Hu C; Li DS\n[Ad] Endereço: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.\n[Ti] Título: "Perfect" designer chromosome V and behavior of a ring derivative.\n[Ab] Resumo: Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.\n8 / 2439 MEDLINE\n[Au] Autor: Wu Y; Li BZ; Zhao M; Mitchell LA; Xie ZX; Lin QH; Wang X; Xiao WH; Wang Y; Zhou X; Liu H; Li X; Ding MZ; Liu D; Zhang L; Liu BL; Wu XL; Li FF; Dong XT; Jia B; Zhang WZ; Jiang GZ; Liu Y; Bai X; Song TQ; Chen Y; Zhou SJ; Zhu RY; Gao F; Kuang Z; Wang X; Shen M; Yang K; Stracquadanio G; Richardson SM; Lin Y; Wang L; Walker R; Luo Y; Ma PS; Yang H; Cai Y; Dai J; Bader JS; Boeke JD; Yuan YJ\n[Ad] Endereço: Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, PR China.\n[Ti] Título: Bug mapping and fitness testing of chemically synthesized chromosome X.\n[Ab] Resumo: Debugging a genome sequence is imperative for successfully building a synthetic genome. As part of the effort to build a designer eukaryotic genome, yeast synthetic chromosome X (synX), designed as 707,459 base pairs, was synthesized chemically. SynX exhibited good fitness under a wide variety of conditions. A highly efficient mapping strategy called pooled PCRTag mapping (PoPM), which can be generalized to any watermarked synthetic chromosome, was developed to identify genetic alterations that affect cell fitness ("bugs"). A series of bugs were corrected that included a large region bearing complex amplifications, a growth defect mapping to a recoded sequence in , and a loxPsym site affecting promoter function of PoPM is a powerful tool for synthetic yeast genome debugging and an efficient strategy for phenotype-genotype mapping.\n9 / 2439 MEDLINE\n[Au] Autor: Mercy G; Mozziconacci J; Scolari VF; Yang K; Zhao G; Thierry A; Luo Y; Mitchell LA; Shen M; Shen Y; Walker R; Zhang W; Wu Y; Xie ZX; Luo Z; Cai Y; Dai J; Yang H; Yuan YJ; Boeke JD; Bader JS; Muller H; Koszul R\n[Ad] Endereço: Spatial Regulation of Genomes, Department of Genomes and Genetics, Institut Pasteur, Paris 75015, France.\n[Ti] Título: 3D organization of synthetic and scrambled chromosomes.\n[Ab] Resumo: Although the design of the synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, the deletion of several classes of repeated sequences and the introduction of thousands of designer changes may affect genome organization and potentially alter cellular functions. We report here the Hi-C-determined three-dimensional (3D) conformations of Sc2.0 chromosomes. The absence of repeats leads to a smoother contact pattern and more precisely tractable chromosome conformations, and the large-scale genomic organization is globally unaffected by the presence of synthetic chromosome(s). Two exceptions are synIII, which lacks the silent mating-type cassettes, and synXII, specifically when the ribosomal DNA is moved to another chromosome. We also exploit the contact maps to detect rearrangements induced in SCRaMbLE (synthetic chromosome rearrangement and modification by -mediated evolution) strains.\n[Mh] Termos MeSH primário: Cromossomos Artificiais de Levedura/ultraestrutura\n10 / 2439 MEDLINE\n[Au] Autor: Zhang W; Zhao G; Luo Z; Lin Y; Wang L; Guo Y; Wang A; Jiang S; Jiang Q; Gong J; Wang Y; Hou S; Huang J; Li T; Qin Y; Dong J; Qin Q; Zhang J; Zou X; He X; Zhao L; Xiao Y; Xu M; Cheng E; Huang N; Zhou T; Shen Y; Walker R; Luo Y; Kuang Z; Mitchell LA; Yang K; Richardson SM; Wu Y; Li BZ; Yuan YJ; Yang H; Lin J; Chen GQ; Wu Q; Bader JS; Cai Y; Boeke JD; Dai J\n[Ad] Endereço: Key Laboratory for Industrial Biocatalysis (Ministry of Education) and Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.\n[Ti] Título: Engineering the ribosomal DNA in a megabase synthetic chromosome.\n[Ab] Resumo: We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in SynXII was assembled using a two-step method, specified by successive megachunk integration and meiotic recombination-mediated assembly, producing a functional chromosome in Minor growth defect "bugs" detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a synXII strain that would be identified as by standard DNA barcoding procedures.\nCromossomos Artificiais de Levedura/ultraestrutura


url: =Dalian Jufeng Mining Group Co.,Ltd, Aluminum Ingots, Antimony Ingots Products Seller\nPhone: +86 - 0319 - 5238671/+86 - 13383690157\nBuilding 3, No. 5 unit Shanhai Yi King Village,Yingchengzi Rord,Ganjingzi District Dalian City Liaoning Province,China\nWeb Page Link: http://www.toboc.com/dalianjufengmininggroupcoltd\n"Dalian Jufeng Mining Group Co.,Ltd" is listed under following Categories:\nEstablished in 1996, Dalian Jufeng Mining Group Co.，Ltd is the large-scale state-owned enterprise directly affiliated with china Government, which is also among one of the 520 enterprises and Chinese top-500 enterprises strongly supported by the State Government. Depending on its superior geological and historical advantages, Dalian Jufeng Mining Group Co.，Ltd owns not only the most comprehensive industrial chain, the largest and highest concentrated gold base with advanced professional technology, but mining group and resources reserve well-known home and abroad, with gold production, resources reserve, economic benefit, scientific level and talent advantages among the top within the same field in China.


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