If Wikipedia is up-to-date, then Buenos Aires has a good claim to having the oldest subway coaches in commercial service in the world, built between 1913 and 1919.
They have been running on line
A since its inception.
These trains were being phased out as of 2010; I'll let someone with a better grasp of Spanish determine whether they are still running now.
I thought some impoverished East European cities had old stock, but they all seem to have renewed anything pre-1970.
The London Underground ran A60 and A62 for about 50 years, since the early 1960s.
These trains were due to be phased out in the early 2010s; the TfL plans called for A stock lasting until 2015, but the replacement seems to have been expedited.
The Metropolitan line might still be running some.
In the Tube, the 1959 stock is no longer doing passenger service but is still used for deicing in winter.
Pyongyang runs trains imported from Berlin, both West and East.
The Dora trains were built between 1957 and 1965 and are now running a quarter way around the world from their youthful home.
If you're willing to accept streetcars, then Hong Kong is still running rebuilt cars from the 1950s (older cars from the 1930s have been scrapped).
The Isle of Wight line (in England) still has 1938 ex-London Underground rolling stock in active service.
travel.stackexchange.com/users/277/Gilles 'SO- stop being evil'
The Schwebebahn suspended monorail in Wuppertal, Germany, not far from Cologne, still trots out Emperor Wilhelm's Kaiserwagen , built in 1900, for special occasions and tourist charters.
It's not in daily service though.
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They’re not exactly the oldest and they are not a subway but an S-train, but they still fulfil the remaining requirements somewhat, especially since they use third rail electrification: The S-Bahn in Berlin still has four historic EMUs that will operate for touristic reasons.
Two are class ET 165 as seen in the image (taken from Wikipedia ).
They were originally introduced in 1928 and actually ran in daily traffic until the 1990’s.
The two that are remaining have been kept in different modifications: One is still in the original 1928 interior design according to Wikipedia.
(The other one has a 1950’s interior.)
Another two historic EMUs are class 167 .
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It's above ground
and it's not in regular service, but the good folks at Market Street Railway in San Francisco occasionally roll out streetcar
No. 578 , built in 1896.
I rode it last September: http://imgur.com/gallery/zw3fP
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They are DMU's with two axles used only by CFR (national rail company), built from 1935 to 1950, first refurbished in the 60s changed their motor from a Ganz of 130 hp to a Raba-Man of 180 hp, at the second main refurbishment from 2008
some of them got a new motor a Volvo Penta 226 hp, and a new paint scheme.
More details (but in romanian) http://transport-in-comun.ro/forum/viewtopic.php?f=70&t=423 more pictures and discussions (in romanian, but if you want to ask something in that forum most people know english) http://www.forumtrenuri.com/t3848p225-automotoare-clasa-77-malaxa
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In Romania, Malaxa railbus class 77, built in the 30s, runs on different routes, with a low number of passagers.
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Besides the antique cable cars, which are a protected National Monument, San Francisco runs historic streetcars on a daily basis.
("F" line.)
Most are the PCC streetcars from the 1940s and 50s.
Some are Peter Witt streetcars originally from Milan, which were built in 1928.
There is one from Melbourne, Australia built in 1929.
travel.stackexchange.com/users/18431/Andrew Lazarus
It's probably not the oldest, and the attraction isn't open yet, but it is possibly the quirkiest: Photo by Richard Pope, CC-BY, from https://www.flickr.com/photos/memespring/311144302/in/photostream/
Mailrail was a narrow gauge, underground railway used for moving post and mail around London.
It closed in 2003, however, at some time in the near future (between 2017 and 2020)
it is going to be reopened for visitors, as part of The Postal Museum : Just down the road, Mail Rail allows the public into the tunnels of the Post Office’s private underground railway for the first time in its hundred-year history.
Visitors can ride a looping mile of track to encounter the industrial heritage and secret geography of the capital.
It's not quite clear exactly which rolling stock and carriages they will be using, however according to this page , the two existing passenger carriages were built upon 1927 and 1930 base units.
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As a rider of various subway/metro/underground systems I was curious where would I find the oldest fleet still in operation.   NYC subway C line has cars built in the mid 60s. I was wondering if there is anything older than that?
Oldest subway/underground/metro rolling stock still in operation
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<trains><public-transport><where-on-earth><factoids>
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Buenos Aires has subway coaches that were built between 1913 and 1919 and are still in commercial service, and the Isle of Wight line still has 1938 ex-London Underground rolling stock in active service. While Hong Kong's streetcars from the 30s have been scrapped, it is still running rebuilt cars from the 50s; San Fransisco also runs historic streetcars on a daily basis in addition to its antique cable cars. In Romania, Malaxa railbus class 77, built in the 30s, runs on different routes, with a low number of passagers. The Schwebebahn  suspended monorail in Wuppertal, Germany, still operates Emperor Wilhelm's  Kaiserwagen , built in 1900, for special occasions and tourist charters. Old rolling stock is in daily use in a number of places, including San Francisco, the Isle of Wight and Buenos Aires. In Wuppertal, Kaiser Wilhelmàs carriage is used on special occasions.
A number of places have old rolling stock still in operation. These include Buenos Aires, Isle of Wight, Romania and San Francisco. Wuppertal brings out Kaiser Wilhelms carriage for special occasions.