Today, biometric technology, which uses physical or behavioural characteristics, could be considered as a reliable individual secure authentication method that helps to determine the identity of an authentic user especially in e-commerce technology. Along with a variety of modalities of biometrics, fingerprints are well-known to be the oldest method used in law enforcement with a verified performance. This paper continues this tradition by using an extending Technology Acceptance Model (TAM). For this work, which is part of a larger body of research, 306 Saudi participants were involved in a large scale experiment, consisting of men and women between the ages of 18 and 55. The experiment included the development of a fingerprint authentication system to examine the differences in male and females and their acceptance of biometric authentication systems within online environments.