Film is considered as a visualized and vivid multimodal communication mode, the cross-cultural dissemination of which cannot be separated from its subtitle translation. Based on the Synthetic Theoretical Framework of Multimodal Discourse Analysis (STF of MDA) put forward by Professor Zhang Delu, the paper analyzed the characteristics and effects of subtitle translation in Wolf Totem from four levels, namely culture, context, content and expression, exploring how different modalities coordinated to construct the whole meaning of the film discourse. After detailed analysis on typical examples, it raised a multimodal analysis model of subtitle translation on the basis of Zhang’s theory, so as to provide a new analytical approach for research on subtitle translation.