@article {Marad171462, author = {Marad, Daniel A. and Lang, Gregory I.}, title = {Restricted access to beneficial mutations slows adaptation and biases fixed mutations in diploids}, elocation-id = {171462}, year = {2017}, doi = {10.1101/171462}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Ploidy varies considerably in nature. Yet, our understanding of the impact of ploidy on adaptation is incomplete. Many microbial evolution experiments characterize adaptation in haploid organisms, but few focus on diploid organisms. Here, we perform a 4,000-generation evolution experiment using diploid strains of the yeast Saccharomyces cerevisiae. We show that the rate of adaptation and spectrum of beneficial mutations are influenced by ploidy. Haldane{\textquoteright}s sieve effectively restricts access to beneficial mutations in diploid populations, leading to a slower rate of adaptation and a spectrum of beneficial mutations shifted towards dominant mutations. Genomic position also plays an important role, as the prevalence of homozygous mutations is largely dependent on their proximity to a recombination hotspot. Our results demonstrate key aspects of diploid adaptation that have previously been understudied and provide support for several proposed theories.}, URL = {https://www.biorxiv.org/content/early/2017/08/02/171462}, eprint = {https://www.biorxiv.org/content/early/2017/08/02/171462.full.pdf}, journal = {bioRxiv} }