TeamNewcastleNanoDrop Spectrophotometer

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NanoDrop Spectrophotometer

Newcastle nanodrop 2.jpeg
Newcastle nanodrop 1.jpeg
Newcastle nanodrop 3.jpeg

Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.

Materials required

  • Pipettes
  • Nanodrop machine
  • Appropriate blanking solution
  • Appropriate samples


  1. Log onto computer and select Nanodrop program from the desktop (ND 1000)
  2. Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.Drop.jpgDrop2.jpgDrop3.jpg
  3. After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (Example: Miniprep samples should be equalized with EB buffer).
  4. Use DNA-50 for DNA samples.
  5. Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
  6. If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
  7. After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.

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