CRISPR system stocks for site-specific mutagenesis

The CRISPR system allows investigators to target double-stranded DNA breaks to specific genomic sequences. A trans-activating RNA (tracrRNA) interacts with a nuclease (Cas9) to direct cleavage to a sequence complementary to an introduced RNA (CRISPR RNA or guideRNA).

Detailed information about the CRISPR system in Drosophila is available from the flyCRISPRFlyCas9 and CRISPR fly design websites.

Tools for designing guide RNAs:
Find CRISPRs from the TRiP
Optimal Target FInder from flyCRISPR
Cas9 Target Finder from FlyCas9