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Chapter 6

2013 Olsson and Westman, licensee InTech. This is an open access chapter distributed under the terms of
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Direct Dissolution of Cellulose:
Background, Means and Applications
Carina Olsson and Gunnar Westman
Additional information is available at the end of the chapter
1. Introduction
Being the main structural component in plants, cellulose is by far the most abundant organic
polymer on earth. The highest quantity of cellulose is found in the secondary walls of higher
plants, such as wood, were the polymer is incorporated in a matrix of lignin and shorter
heteropolysaccharides such as hemicelluloses and pectins. Cellulose is also found in some
fungi and algae, in the marine animal family of tunicates and as an extracellular product of
some bacteria. In theory, cellulose is an inexhaustible resource and yields 1.5x10
tons of
biomass every year. Despite the fact that cellulose is available in any plant, wood pulp
provides the main part of cellulose used today, for example in board, paper and textile.
Historically, cellulose has been used for as long as man has existed, first as fuel and shelter
and later for clothing and writing material such as papyrus. Cotton was domesticated
thousands of years ago and the soft fiber surrounding the cotton seed was used for making
textiles in many parts of the world. Cotton is still the main source of cellulose for textile but
the demand for wood based raw material is increasing due to the environmental drawbacks
associated with cotton cultivation and processing, such as high water and pesticide usage.
Both cotton and wood based cellulose can also be used as the basis for cellulose derivatives
such as cellulose ethers and cellulose esters. These are widely used, for example in
pharmaceuticals, food, construction materials and paint. When it comes to sustainable raw
materials for future demands on fuel, chemicals and materials, both bulk and high
performance, cellulose is definitely a resource of great importance and still today one that is
Corpora non agunt nisi solute (substances do not react unless dissolved) is a statement with
roots in ancient alchemy, and still has some validity today. Unlike many petroleum based
polymers, cellulose will not melt but decompose at elevated temperatures. To get the
cellulose in liquid form, it has to be dissolved or chemically modified. Homogeneous

Cellulose Fundamental Aspects 144
reaction as a route to add functionality to the reactive cellulose hydroxyl groups provides
better control of total degree of substitution and better conditions to control not only the
substitution pattern along the cellulose chain but potentially even the substitution pattern
within each monomer since each part of the polymer is, at least in theory, equally exposed to
reagents and solvent. Due to its chemical composition, cellulose is insoluble or only partly
soluble in most common solvents. Although quite a few alternatives have proven useful
over the years, the search for efficient and non-degrading solvents for cellulose is still
ongoing and in the light of current trends within sustainability and exploration of
alternatives for oil based products, this work is now more important than ever. Suitable
solvents for cellulose are not only essential in homogeneous reactions, but are indispensable
in the characterization of the polymer itself, and last but not least in the shaping of cellulose
into e.g. textile fibers, films and membranes.
One of the most successful routes to dissolving and shaping of cellulose is via the viscose
process. This technique involves conversion of cellulose to cellulose xanthogenate, by a
reaction with carbon disulfide in sodium hydroxide first described by Charles Frederick
Cross, Edward John Bevan, and Clayton Beadle [1]. The metastable cellulose xanthogenate is
soluble in aqueous sodium hydroxide and the resulting viscous liquid is used as a spin
dope. This means that the dissolved polymer is not actually cellulose but a cellulose
derivative. The polymer is then precipitated in acid, for simultaneous neutralization and
regeneration of the cellulose in a wet spinning step. Hence, the resulting regenerated fiber
will be pure cellulose. This process is still today widely used to make viscose textile and
cellophane. The viscose process yields high performance fibers with excellent properties for
various applications, but it has some drawbacks. The sulfuric reagents, their byproducts and
the heavy metals used in the regeneration baths are, despite a decrease in usage, still a
problem from an environmental point of view. Both air and water streams must be
adequately purified to meet todays regulations.
In an attempt to avoid some of the problems associated with the in some sense outdated
viscose process, numerous alternatives have been developed. One method where all existing
technology can be translated directly from the viscose process to new chemistry is the
carbamate process, where urea is used to produce cellulose carbamate, a derivative soluble
in aqueous sodium hydroxide that can be fed into the ordinary viscose line [2]. The process
utilizes alkalized and pre-ripened pulp in accordance to the viscose process. In this case the
spin bath for the wet spinning step can be either acidic or alcoholic to yield the pure
cellulose. The urea route to soluble cellulose has a lower environmental impact and lower
energy consumption than the traditional viscose process. Commercial plants are not yet in
use but Neo Industrial Plc's viscose fiber division, Avilon Ltd, plans to convert a viscose
production site to the new urea technology and should open 2013, according to their press
release in January 2011 [3]. One major advantage with the carbamate route is that the
intermediate cellulose carbamate is stable and may be stored or transported without
significant degradation.

Direct Dissolution of Cellulose: Background, Means and Applications 145

Figure 1. Cellulose xhantogenate (left) with R = H or xhanthogenate group and cellulose carbamate
(right) with R = H or carbamate group.
Common to the cellulose/solvent systems in both the viscose process and the carbamate
process is that they involve an intermediate polymer. As already stated, it is in fact not the
cellulose itself but a cellulose derivative that is dissolved. This is true for many other solvent
systems for cellulose as well, such as DMF/N2O4 or DMSO/N2O4, CF3COOH, HCOOH/H2SO4
and Cl2CHCOOH. This case may or may not be convenient depending on the application.
Direct dissolution of cellulose, without derivatization, is not only necessary in some
analytical applications but may also simplify the production of regenerated cellulose since
many steps are omitted. A direct solvent will also be considerably more straightforward to
recycle since no byproducts are generated, at least not in theory. Today, the only really
successful direct solvent system for regenerated cellulose on large scale is the NMMO
hydrate even if e.g. both alkali and acid systems are proposed for the same use.
When discussing the dissolution of cellulose in general, and the direct dissolution of it in
particular, it is necessary to have a proper understanding of the polymer itself. The
properties of cellulose are interesting and unique in many ways. Its dissolution process
must also be seen as a result of both kinetics and thermodynamics and as a multidisciplinary
chemical challenge, where knowledge in both inorganic and organic chemistry as well as
physical chemistry and process engineering is required. To understand, and above all
control the dissolution process will be of great importance for future applications of the
cellulose polymer.
2. Macromolecules
Though the use of both synthetic and modified natural polymers have been of great
significance in industry since the 19
century, a reasonably correct understanding of
polymer structure was not developed until in the 1920s when Hermann Staudinger
proposed a model where polymers, for example cellulose, was composed of covalently
linked small repeating units, i.e. the very definition of a polymer today. For this work he
received the Nobel Prize in chemistry 1953.
Polymers, with few exceptions, are not homogeneous in length. The degree of
polymerization (DP) varies and the molecular weight distribution is one of the major factors
influencing the properties of a given polymer.
Some polymers, such as starch, can appear in linear or branched forms. This has great
influence on many properties such as crystallinity, solubility, density and as a consequence
of this, even thermal and mechanical properties that have direct influence on the industrial

Cellulose Fundamental Aspects 146
applications of the product. The repeating units of any polymers are not restricted to being
of the same kind all through the chain length. Nor are the branches restricted to being of the
same type as their backbone. Alternating monomers yields so called co-polymers or
heteropolymers, that can be randomly organized or well defined. Homopolymers may vary
in stereochemical configuration to yield isotactic, atactic or syndiotactic polymers if there are
any substituents as seen in Figure 2.

Figure 2. Representation of an isotactic polymer (top) where all monomers have the same
stereo/regiochemical configuration, a syndiotactic polymer (middle), where the monomers have
alternating positions along the chain and an atactic polymer (bottom) where the configuration of the
monomers is random.
Depending on chemical and stereochemical design, polymers experience different interchain
forces that, without actual covalent bonding, keep the polymer chains together in the solid
state. For electrically neutral polymers, the strongest and most important interchain force is
of course hydrogen bond interactions, but dispersion forces may have a significant effect on
the polymer bulk too.
3. Solubility of macromolecules
In some aspects macromolecules fall under the same rules as all substances when it comes to
solubility, in others not at all. Non-polymeric materials dissolve immediately, and in a good
solvent the process is controlled mainly by external mass transfer in a stagnant zone near
the solid surface. Polymers, on the other hand, undergo a multistep process going from solid
to dissolved state. Mass transfer is bidirectional as solvent penetrates the polymer network
and swell its outer layer while polymer disentangles from the outermost surface and
transfer into the liquid bulk.
As can be intuitively understood, the dissolution rate of polymers decreases with increasing
molecular weight. A more intriguing fact is that polydispersity too has a significant effect on
the dissolution rate. This effect was investigated in polymethyl methacrylate solutions by
Manjkow and others in the late 80s. Polymers of broad molecular weight distributions
(Mw/Mn 2) dissolved twice as fast as the monodisperse equivalent with the same Mn [4].
Pure polymer Infiltration
Solid swollen
Gel layer Liquid layer Pure solvent
Figure 3. Schematic picture of the composition of the surface layer, adapted from Miller Chou [5].

Direct Dissolution of Cellulose: Background, Means and Applications 147
Due to the intrinsic high molecular weight, polymers in general are soluble only in selected
solvents. As a result of interchain forces, solubility of polymers is not only dependent on
chain length, but also chain-to-chain interaction. Crystallinity and polar groups that may
take part in hydrogen bonding thus play a major role in solubility and reactivity of any
The discussion on thermodynamics of dissolution must begin with Gibbs free energy
0 = E IS (1)
and the idea of an ideal solution, where the enthalpy of mixing and the volume change upon
mixing is equal to zero. In a binary solution this would mean that there is no energy gain or
loss for the constituents in the solution to interact with either a molecule of its own kind, or
a molecule of the other type. This is of course never really true but has some relevance as a
simple model. In the case of polymers in solution, the difference in size leads to a non-ideal
mixture. In addition to enthalpy, one must take entropy into consideration. For a
macromolecule, the gain in entropy is not as great as for the corresponding small molecule.
Furthermore, the monomer-to-monomer covalent bond restricts motion and confines the
macromolecule within the solvent. This is especially true for stiff polymers, where the
conformational freedom does not increase to the same extent as it does for freely rotating
polymers when going from solid to dissolved state.
Dissolution can only take place if the dissolved state represents a lower energy than the
solid state, meaning that enthalpy of mixing must be balanced by the entropy term. Again,
consider glucose and cellulose. The same type of interactions should take place between a
glucose molecule and its solvent as between a polymer of glucose and its solvent. The gain
in entropy for glucose monomers is far greater than the entropy gain for the glucose
polymer. Hence, glucose dissolves easy in e.g. water, while cellulose does not.
A well needed addition to the Gibbs energy equation is the Flory Huggins model (Equation
2) of the thermodynamics of polymers in solution. The free energy of mixing is described by
= RI|n

] (2)
where Gm is the change in Gibbs free energy, R is the ideal gas constant, T is temperature,
n1 is the number of moles of the solvent, 1 is the volume fraction of solvent, n2 is the
number of moles of polymer and 2 is the volume fraction of polymer. The parameter chi ()
describes the energy of interdispersing polymer and solvent molecules, i.e. both enthalpic
and entropic deviations from ideality. The volume fraction is analogous to the molar
fraction, but takes into consideration the size of the molecule. For a small solute, with a size
comparable to the size of the solvent molecule the volume fraction can be replaced by molar
fraction [6].
This model does not take into account a possible change in volume due to mixing, nor is it
applicable in the case of very dilute solutions. For these cases, the theory of excluded
volume is used. This is based on the simple assumption that one part of a polymer chain

Cellulose Fundamental Aspects 148
cannot occupy the space that is already occupied by another part of the chain. However, the
size and shape of dissolved polymers depends on the quality of the solvent. A good solvent
is a solvent that coordinates well and strong to the polymer whereas a poor solvent only has
weak or almost no coordination to the polymer. The polymer will stretch out in a good
solvent and occupy a large volume since interactions with the solvent molecules are
favorable. The polymer will contract in a poor solvent, and if the solvent is too poor,
eventually precipitate. The so called theta point is reached at the exact conditions where the
excluded volume effect is balanced by the solvent. In a theta solvent, the polymer chain
reverts to its ideal characteristics, and the chain dimensions are no longer dependent on the
solvent choice. In other words, the polymer interacts with the solvent as it would interact
with itself, and will have the same chain dimensions as it would have in a polymer melt or
amorphous solid phase. This means that analytical measurements in a theta solvent will be
independent of solvent and give the true value of e.g. radius of gyration [6].
Apart from chemical compatibility, i.e. thermodynamic features of the solvent/solute
system, there is kinetics to consider. In the case of a macromolecule such as the cellulose
polymer, this is a parameter of critical importance since diffusion rate is ideally inversely
proportional to the size of the particle, or in this case the molecule. Hence, in the case of
entangled polymers with a severe tendency to self assemble the diffusion rate is drastically
retarded. It matters not if the solvent is thermodynamically ideal, if the rate of dissolution is
too slow and a solution does not form under a desired amount of time the solvent is useless.
Kinetics of dissolution can be manipulated by many different means. Diffusion of large
molecules is slow, but addition of heat speeds up the process. In general, addition of heat
also lowers the viscosity of liquids such as the solvent and this will be advantageous since it
will lower the diffusion resistance. Furthermore, mechanical energy added to the system in
form of shear stress will decrease the length of the diffusion path from concentrated
polymers solution at the particle surface to the bulk solution. This will not affect the rate of
diffusion, but the flux of molecules and thereby the overall kinetics of the dissolution
process. This can be understood in a simplified way using Ficks first law shown in equation
3 as linear integrated version.
[ =
J is the flux [mol/s], D is the diffusion coefficient [m
/s], c is the concentration gradient
from the undissolved particle surface to the bulk solution and x is the distance from the
particle surface to the bulk, the stagnant layer where mass transport is controlled by
diffusion only. Thus, if the distance from the particle surface to the bulk is diminished, the
flux of molecules from the solid particle to the bulk solution will increase. It must be
stressed that kinetics of polymer dissolution is not by any means as simple as kinetics of
dissolution of small molecules, especially when it comes to semi-diluted or concentrated
solutions. For example, as the concentration of dissolved polymer increases the viscosity of
the solution will increase and this will slow down the process. A basic understanding of the
kinetics in a simple system can nevertheless come in handy when it comes to understand the
processes in more complex ones.

Direct Dissolution of Cellulose: Background, Means and Applications 149
4. Cellulose morphology
To comprehend the interactions of cellulose and its solvent as well as the dissolution process
it is important to have a clear understanding of the polymer itself. Unlike some other
biopolymers, like proteins, cellulose in its native form is highly polydisperse and often
closely linked to other compounds, e.g. the lignin and hemicelluloses in wood. Apart from
this, the polymer structure provides, though at a first glance very symmetric and simple,
serious challenges due to its chemical composition and spatial arrangement. From monomer
to bulk material, all levels present interesting features that are highly important.
Cellulose was first defined as the remaining part of plant tissue after purification and in the
early days of cellulose technology the word cellulose was used also for what is today
defined as pulp [7]. Eventually this residue was found to be a carbohydrate very similar to
starch, and today it is known that it is a linear syndiotactic polymer (cf. Figure 2) of
-1,4-glycosidic bond linked D-glucopyranose units, where every other monomer is rotated
180 with respect to its neighbors. This structure can also be defined, if instead pairs of
glucose units, cellobiose, are defined as the repeating unit, as an isotactic polymer. The
individual glucose monomers in the cellulose polymer are often referred to as
anhydroglucose units, AGU, due to the loss of one water molecule in the polymerization
where a hemiacetal reacts with an alcohol to form the acetal bond. From crystallographic [8-
9] and NMR spectroscopic data [10] it has been shown that the six membered glucose
monomer takes on a chair conformation. Three hydroxyl groups are present in the AGU,
secondary on C2 and C3, and a primary on C6. The rotational conformation of the primary
alcohol on C6 can be altered, and this has a profound impact on the hydrogen bonding
pattern and therefore the crystallinity of the cellulose metastructure [11]. In one end of the
polymer the anomeric carbon is involved in a glycosidic bond. This is referred to as the non-
reducing end. In the other end of the polymer the anomeric carbon is free to convert to an
aldehyde structure, and these two states are in equilibrium. This end is referred to as the
reducing end. This chemical polarity of the cellulose chain is important when it comes to its
different crystal structures.

Figure 4. The cellulose polymer chain structure

Cellulose Fundamental Aspects 150
The number of AGU units defines the degree of polymerization (DP) of the cellulose.
Depending on source, this might vary to a great extent. The DP of various sources including
native wood was first determined 1962 [12] to between 9000-10000. Extraction methods and
treatment of cellulose might degrade the polymer, and the DP of wood pulp varies between
300-1700 whereas regenerated fibers from cellulose have a degree of polymerization no
greater than 250-500.
Polymers in native cellulose are densely packed in a semi ordered structure. Intramolecular
hydrogen bonds provide chain stiffness, and intermolecular hydrogen bonds allow for the
linear polymers to assemble in sheet structures. These sheets are further packed by
hydrophobic interactions into crystals. Crystalline regions in silk, bamboo and wood were
confirmed by X-ray spectroscopy already 1913 [13] and ever since, the crystalline structure
of celluloses has been studied thoroughly. To this day four main different crystal structures
of cellulose have been discovered, namely celluloses I, II, III and IV. Based on the X-ray
diffraction data for specimens from the sea algae Valonia ventricosa, native cellulose I was
first said to have a triclinic crystal structure of parallel chains [14] but later work showed by
solid state NMR spectroscopy that cellulose I can be subdivided into cellulose I and
cellulose I [15-16], where the I is dominating in bacteria and algae and I is dominating in
higher plants. Recent work on X-ray and neutron diffraction data suggests that only
cellulose I is truly triclinic, while the I form is monoclinic [17]. Cellulose I can easily and
irreversible transform into the monoclinic cellulose II by regeneration or alkali treatment,
suggesting that the cellulose II is the more stable allomorph [18]. In this transformation, the
parallel ordering of polymer chains found in cellulose I is replaced by what seems to be an
antiparallel configuration in cellulose II [19]. It is still debated whether this antiparallel
ordering is due to chain-folding or interdiffusion of crystallites. Cellulose III and cellulose
IV can be reversibly prepared from either cellulose I or cellulose II by various chemical
treatments [20].
In its native form, cellulose crystals are never pure. Crystalline regions are interrupted by
less ordered structures. Several suggestions on how these crystalline and non-crystalline
regions are intermixed have developed over the years, such as single crystals [21] or
uniform elementary fibrils [22] but nowadays a so called fringed fibrillar model, developed
in the late 50s [23], of the material is widely accepted [24]. In this model the microfibril is not
regarded a single crystal but rather a less structured arrangement of non-uniform crystalline
segments accompanied by amorphous parts, both longitudinal and lateral.
5. Solubility of cellulose
Being a linear homopolymer, cellulose does not appear as a complex structure but its
dissolution is in fact very complicated. Many factors contribute to make the cellulose
macromolecule insoluble in most common solvents. Cellulose in its native form is often a
very long polymer and high DP inevitably leads to decreased solubility due to decreased
entropic gain in the dissolution process as already discussed. Glucose, cellobiose and any
oligomer of cellulose structure up to around a DP of less than 10 is soluble in simple

Direct Dissolution of Cellulose: Background, Means and Applications 151
solvents such as water and cellulose is not, so this is indeed a relevant factor.
Furthermore, the interactions between the cellulose molecules provide a challenge. Three
hydroxyl groups per AGU make complex patterns of hydrogen bonds possible. To break
these, solvents with high hydrogen bonding capacity are necessary. Quite a few liquids
including water are able to swell, but not to dissolve cellulose. Swelling is defined as a
process where the liquid molecule penetrates cellulose structure by interacting with the
polymer to a certain extent, leaving the volume and physical properties of the cellulose
significantly changed but the solid, or semi-solid state remains. Dissolution, on the other
hand, completely destroys the supramolecular structure due to superior interaction
between solvent and polymer, and results in a clear solution where the polymers are
molecularly dispersed. The same liquid may act as a solvent or merely a swelling agent,
depending on the conditions used in the experiment. Temperature can be such a
determinant parameter.
One way to estimate a new solvents compatibility is to evaluate the hydrogen bonding and
polarizability properties of the solvent. Parameters of the empirical KamletTaft
solvatochromic relationship are used to calculate the hydrogen bond donor (), hydrogen
bond acceptor (), and dipolarity/polarizability (*) properties of solvents as contributing to
overall solvent polarity. This is done by UV/Vis spectroscopy to monitor the interactions of
the substrate with chosen indicators of e.g. the ones shown in Figure 4. Numerous
solvatochromic indicator dyes are available [25]. In many reports where new solvents for
cellulose are being evaluated, the Kamlet-Taft parameters are held as highly important [26-
29]. In Table 1 the solvatochromic parameters , and * for cellulose, a cellulose non-
solvent and a cellulose solvent are compared.

Cellulose (dried) 1.27 0.60 0.41
H2O 1.17 0.47 1.09
DMAc/LiCl (15%) 0.54 2.0 0.67
Table 1. Kamlet-Taft parameters for cellulose, water and the cellulose solvent system
dimethylacetamide (DMAc) with 15 % LiCl [26, 30-31].

Figure 5. Examples of probe dyes for determination of solvatochromic parameters of cellulose solvents.

Cellulose Fundamental Aspects 152
Water cannot be used to break the bonds in cellulose, yet both water-water, carbohydrate-
water and carbohydrate-carbohydrate hydrogen bond strengths are around 5kcal/mol [32].
Many forget to take this question into account when they reason about the dissolution of
cellulose and its relative insolubility. It is obvious that hydrogen bonding is not the only
relevant factor in the quest for a suitable solvent, yet the amphiphilic nature of cellulose is
still widely overlooked. The last few years, a few eye-opening papers have taken this matter
up to discussion [32-37].

Figure 6. Hydrogen bonds arrange cellulose chains in sheets, and the stacking that forms a three
dimensional structure involves hydrophobic interactions.
A relatively new approach when trying to understand molecular interactions is
computer simulations and modeling. Some very recently published papers describe this
method, showing molecular origins of the recalcitrance of biomass in the terms of
decrystallization and dissolution, and showing quantitatively the need for taking
cellulose amphiphilicity into account [36-38]. For comparison it may be useful to study
cyclodextrins. Typical cyclodextrins are oligosaccharides made up from six to eight 14
linked monosaccharides in a torus and are produced from starch but since starch as
cellulose is built up from glucose, this may still serve as a model. Cyclodextrins are
water soluble, but the arrangements of hydroxyl groups provide a pronounced
The inclusion or the interior of the sugar macro ring is if not fully hydrophobic at least
significantly less hydrophilic than the outer part of the structure. As a result of this,
cyclodextrins may serve as the host in inclusion complexes. Unusual hydrogen bonding
patterns take place inside the cyclodextrin, due to the confined space and the hydrophobic
nature of the torus, which was shown by Bezel et al. in a neutron scattering study [39]. This
relates to and partly explains the concept of high energy water i.e. the water trapped in
the cyclodextrin ring in a water solution which upon release by addition of a more
hydrophobic substance contributes to a favorable enthalpy change. The driving force for
inclusion of a hydrophobic substance in a cyclodextrin ring should be considered an
atypical hydrophobic interaction, since it is not entropy driven [40]. An extensive
overview of cyclodextrins as inclusion complexes was published by Clarke et al. and
explains many aspects of the amphiphilicity of these substances [41].

Direct Dissolution of Cellulose: Background, Means and Applications 153
The alpha, beta and gamma cyclodextrins with six, seven and eight glucose units in their
ring as shown in Figure 6 have all been granted GRAS (generally recognized as safe)-status
by the American Food and Drug Administration (FDA). This is useful for many purposes
where a hydrophobic compound, or part of a compound, needs to be solubilized in water
for example in the drug delivery of lipophilic substances. By considering the uses of
cyclodextrins, it is obvious that polymers of glucose should have amphiphilic traits. The
difference is that these polymers are linear instead of torus shaped, and make up sheets
where the hydrophobic interactions are orthogonal to the hydrophilic.
Coming back to the discussion of polymer solubility, it now seems obvious that any
reasonable solvent for cellulose is one that must overcome the low entropy gain by excellent
solvent polymer interactions. For cellulose that has both hydrophilic and hydrophobic
features, i.e. the equatorial OH-groups and the axial CH groups, a good solvent should
contain both a hydrogen bonding part and a hydrophobic part.

Figure 7. The molecular structure of the six membered alpha cyclodextrin (-CD), the seven membered
beta cyclodextrin (-CD) and the eight membered gamma cyclodextrin (-CD).
Apart from this, the kinetics of cellulose dissolution in a chosen solvent may be controlled
by elevated temperatures, at least up to the point where side reactions such as solvent
and/or cellulose will start to degrade. In industrial systems, mechanical energy is added as
cellulose dissolution takes part in kneaders that speed up dissolution rate and helps in the
disintegration of fibers and gel particles that have been formed as a result of initial swelling.
Co-solvents may be added to lower the viscosity of the solutions.
6. Solvents for cellulose
Despite the challenges associated with the dissolution of cellulose, numerous options are
available. Here the focus lies on direct solvents, i.e. solvents that do not form covalent bonds
with the polymer but merely interact by physical means. This distinction between non-
derivatizing and derivatizing solvents must be made in order to choose the correct solvent
for a specific use. Solvents may further be classified as aqueous or non-aqueous, or as
containing halogens or not or whatever else suits the users purposes.

Cellulose Fundamental Aspects 154
Solvents can be further classified based on their number of components. Both
unicomponent, bicomponent and tricomponent solvent systems are known and including
solvent mixtures the range is even broader. Since many non-derivatizing, or direct, solvents
for cellulose includes water, as bulk solvent or as bound water in a salt or organic hydrate, it
is not fully satisfactory to make a binary distinction between aqueous and non-aqueous
solvents. Instead, solvents could be placed on a continuous scale that represents the amount
of water in the system. However, herein the binary view is maintained.
6.1. Aqueous media
Being the most green of all chemicals, water is of course an attractive solvent and reaction
media. In fact, pure water can actually be used in the field of cellulose technology. Water at
around its critical point demonstrates some unique properties and can be an interesting
media for various chemistry. Dissolution and hydrolysis of cellulose in subcritical and
supercritical water was investigated by Sasaki et al. [42] but the dissolved cellulose in this
case was probably shorter fragments and cellooligomers since severe depolymerization
must take place before any dissolution can be expected as stated by more recent publications
[43-44]. This means that however appealing, it is not yet possible to dissolve high DP
cellulose in pure water. Water based solvent systems are plentiful though, and different
additives can make water into a very efficient dissolution media. Some of the many
alternatives available are listed below.
6.2. Aqueous alkali
Mercerization, the process of soaking cellulose in strong alkali to the extent that the crystal
structure changes from cellulose I to cellulose II, is one of the most technically relevant
processes in cellulose technology and is used to activate the hydroxyl groups of cellulose for
further modification and/or dissolution. Cellulose is partly soluble in an aqueous solution of
sodium hydroxide in a concentration of about 10 % w/w. The amount of cellulose that is
soluble in NaOH/H2O depends on degree of polymerization and also mode of crystallinity
[45]. Isogai investigated the solubility of cellulose from several sources but never succeeded
in preparing cellulose solutions of higher concentrations than 5 % [46]. A method for
complete dissolution of cellulose in lye solutions was also patented in 1994 [47]. For
cellulose to dissolve in alkaline aqueous media, it needs to be cooled well below room
temperature. Soube et al. completed the phase diagram for the ternary system
cellulose/NaOH/H2O [48]. Taking the amphiphilic properties of cellulose into account, it is
not surprising that it has been shown that cellulose in NaOH/H2O is in fact not completely
dissolved but forms aggregates [49].
More recently, sodium hydroxide solutions with different additives have turned out to
dissolve cellulose more efficiently than the binary NaOH/H2O system itself. Such additives
are for example poly(ethylene glycol) (PEG) [50-51] and urea [52] and/or thiourea [53-55].
The fact that sodium hydroxide, PEG and urea are all environmentally friendly and pose
low toxicity towards humans and animals makes these solvent systems interesting for large

Direct Dissolution of Cellulose: Background, Means and Applications 155
scale applications. However, mixed systems always pose high demands on recovery
systems, and the amount of additives needed in these systems makes recovery and reuse
quite necessary.

Figure 8. Additives in aqueous alkali solvents for cellulose. From left to right; poly(ethylene glycol),
urea, thiourea.
Mechanical or chemical pretreatment is necessary for the dissolution to be efficient enough
for industrial needs [56]. Several studies of cellulose in the aqueous NaOH/urea system
using e.g.
H NMR, FTIR, small angle neutron scattering and wide
angle X-ray scattering suggest that the dissolution mechanism is based on the hydrates of
NaOH that in low temperatures are able to form hydrogen bonds with the cellulose chain,
while the urea molecules surrounds the cellulose/NaOH/H2O inclusion complex, screening
it from other cellulose molecules and thereby prevent cellulose aggregation [57-59]. Being a
hydrotrope, urea is expected to increase the solubility of a poorly water soluble solute in
aqueous solutions. This is explained by the ability of hydrotropes to break water structures,
i.e. the effect is explained by urea water interactions, and/or a tendency of hydrotropes to
interact with the solute itself by hydrophobic interactions. Interestingly, in the case of
cellulose in aqueous NaOH/urea, no direct evidence of interaction between urea and
cellulose was found. In the original NaOH/PEG/H2O publication by Yan and Gao [50] the
dissolution mechanism was suggested to be similar to that in NaOH/urea/H2O. It was
proposed that the solution is stabilized by polyethylene glycol chains, here acting as
hydrogen bonding acceptors. It has also been suggested that the mechanism for dissolution
in these types of solvent mixture relates to a charging up of the cellulose, i.e. turning it into a
polyelectrolyte [32].
The solutions of cellulose in aqueous alkali with urea and/or thiourea are being thoroughly
investigated for shaping purposes, i.e. regeneration of cellulose into fibers, membranes or
similar. Recently, Yang et al. prepared high performance flexible films from different
cellulose sources in a solvent of alkali and urea [60]. The films showed good gas barrier
properties over the entire relative humidity range, and always one order of magnitude
lower gas permeability than cellophane films which are prepared via the viscose process.
Compared to conventional films from poly(ethylene) and poly(propylene), all cellulose films
show increased water vapor permeability, but this is expected due to the intrinsic
hydrophilicity of the polysaccharide structure. Regenerated cellulose fibers similar to
viscose or Lyocell fiber have been wet spun from solutions of NaOH/urea [61] and
NaOH/thiourea solutions [62-63].
The procedure for dissolving cellulose in aqueous alkali/urea-systems is based on a freeze-
thaw method. The solvent is pre-cooled to below the freezing point of water before cellulose
is added and kept cold until complete dissolution of the polymer [64]. Regeneration takes

Cellulose Fundamental Aspects 156
place by precipitation in e.g. dilute H2SO4, ethanol, butanol or acetone [60]. Lithium
hydroxide and potassium hydroxide are expected to have a similar effect as sodium
hydroxide. A strong impact of the choice of salt has been observed, and the dissolution
power of the different systems is in the order LiOH/urea > NaOH/urea >> KOH/urea. Both
LiOH and NaOH in combination with urea are able to rapidly dissolve cellulose, while
KOH is not [64].
The possibility to perform homogeneous reaction in aqueous alkali/urea systems has not
been neglected and it has been shown to be a stable media for etherification reactions.
Carboxymethylation of cotton linters and microcrystalline cellulose was carried out in
lithium hydroxide with urea and the degree of substitution could be controlled [65]. The
product was water soluble CMC. Another important cellulose derivative, namely
hydroxyethyl cellulose, was prepared from cotton linters by homogeneous reaction in
sodium hydroxide with urea under mild conditions [66].
6.3. Inorganic metal complexes
One interesting group of solvents is the one of inorganic metal complexes. The complexes
are of transition metal ions and nitrous ligands. Some of the most common ones [67] are
listed in Table 2.
As in many cases with solutions of transition metal complexes, most of the cellulose solvents
in this group are strongly colored. This is true for example with Cuoxam, Nitren and Cuen
which all are deep blue, while Pden only displays a weak yellow color. The color of the
solvent or in some cases the cellulose metal complex in itself may cause problems in
analytical applications where light scattering or refractive index is used [68].

Cadoxen [Cd(H2N(CH2)2NH2)3](OH)2
Cdtren [Cd(NH2CH2CH2)3N](OH)2
Cooxen [Co(H2N(CH2)2NH2)2](OH)2
Cupren [Cu(H2N(CH2)3NH2)2](OH)2
Cuam/Cuoxam/Schweitzers reagent [Cu(NH3)4](OH)2
Cuen [Cu(H2N(CH2)2NH2)2](OH)2
Nioxam [Ni(NH3)6](OH)2
Nioxen [Ni(H2N(CH2)2NH2)3](OH)2
Nitren [Ni(NH2CH2CH2)3N](OH)2
Pden [Pd(H2N(CH2)2NH2](OH)2
Zincoxen [Zn(H2N(CH2)2NH2)2](OH)2
Table 2. Transition metal complexes with amines or NH3, their common names and chemical formula.
Saalwchter et al. compared several metal complexes and found that the coordinative
binding metal complexes such as Cuoxam, Nitren and Cdtren were most efficient as
cellulose solvents. The coordination takes place at the deprotonated olate anions at C2 and
C3 at each AGU. Even though the dissolution appears to be to molecular level and via

Direct Dissolution of Cellulose: Background, Means and Applications 157
interaction with the C3 hydroxyl group, the cellulose chain stiffness appears to be
considerable. The number of Kuhn segments, i.e. the number of efficient straight segments
seconded by kinks, per polymer rarely exceeds 50 [68] which means that several monomers
are assembled in rod like structures before an actual bend.
Apart from complex coordination, metal complexes can interact with cellulose via pure
Coloumb interactions. This is the case for Cuen [68], but not for Pden which forms square
planar complexes with the AGUs of cellulose as with any other ligand [69-70].
Aqueous inorganic salts or metal complexes can also be used for dissolution and
regeneration of cellulose. The Cupro process, using cuprammonium hydroxide (Cuam) as
solvent, was invented already in the year 1890 and is still used today, although not in a huge
scale [71]. The solvents have also proven viable reaction media. For example, completely
homogeneous etherification of cellulose may be performed in Nitren to produce
carboxymethyl cellulose in a one-phase-reaction with a regioselectivity and substitution
pattern similar to the carboxymethyl cellulose obtained from commercial routes in NaOH
slurries [72]. Unfortunately, some of the representatives of the inorganic metal complex
based solvents initiate severe cellulose depolymerization in the presence of even traces of
oxygen [73].
6.4. Molten inorganic salt hydrates/concentrated inorganic salt solutions
Molten inorganic salts have been pursued as cellulose solvents and reaction media for close
to 100 years now and some are indeed able to dissolve cellulose without pretreatment and in
reasonable concentrations. Both pure salt hydrates and mixtures of different salt hydrates as
well as certain concentrated inorganic salt solutions may swell or dissolve cellulose [74]. The
most commonly used solvents in this class are MgCl2*6H2O, LiCl*5H2O, LiClO4*3H2O,
ZnCl2*4H2O, ZnCl2/H2O, LiSCN and Ca(SCN)2/H2O with or without adition of the sodium
or potassium thiocyanate salt [75]. LiClO4*3H2O is an extremely efficient solvent and
cellulose in this particular salt gives clear solutions within a few minutes.
The dissolution process of cellulose in the aqueous Ca(SCN)2 solvent system was examined
using IR spectroscopy by Hattori et al. and it seems to be based on complex formation and
an addition compound between cellulose and the thiocyanate anion [76] but curiously
enough, the crystallinity of regenerated cellulose product appears to depend on what kind
of precipitation agent is used. In water, only cellulose II is recovered, but in methanol the
product is mainly cellulose I. Upon precipitation in acetone the product is amorphous [77].
The interaction between cellulose and salt hydrates have also been examined using Raman
spectroscopy [74], and of course different NMR spectroscopy techniques [78]. The variation
in dissolution processes in different molten salt hydrates was again thoroughly investigated
by e.g.
C NMR spectroscopy and discussed by Leipner et al [79].
Homogeneous reactions to obtain cellulose derivatives may be carried out in molten
inorganic salt hydrates. Heinze with coworkers reported successful carboxymethylation of
cellulose in LiClO4*3H2O with varying degree of substitution, and a statistical distribution of

Cellulose Fundamental Aspects 158
substituents, showing that no part of the cellulose polymer was inaccessible to the reagents
[72, 80]. Esterification in the form of acetylation in the molten inorganic salt LiClO4*3H2O
and the eutectic mixture of NaSCN/KSCN/LiSCN*2H2O, as well as deacetylation reactions
of cellulose triacetate in the concentrated salt solution of ZnCl2/H2O has been reported [81].
The acetylation reactions were performed with various acetylating agents such as vinyl
acetate and acetic anhydride.
6.5. Acidic solutions of cellulose
It is sometimes claimed that mineral acids are able to dissolve cellulose. However, this must
be put in context. Dilute acids swell cellulose, but dissolution can only be achieved using
higher concentrations of acids and is expected to be associated with severe, if not complete,
chain degradation over time if temperature is not kept very low. The concept of level-off
degree of polymerization (LODP) was introduced as the chain length of cellulose after
treatment in 2.5 N hydrochloric acid [82-83]. If the hydrolysis is allowed to continue the
degree of polymerization will eventually reach the LODP which is thought to reflect the
longitudinal size of the native cellulose crystals, since non-crystalline areas are preferably
hydrolyzed over the crystallites.
Concentrated mineral acids such as hydrochloric acid, phosphoric acid or mixed acids are
utilized as degradation media to prepare cellodextrins, short chained cellulose oligomers
[84-87]. Phosphoric acids, with or without additives such as organic acids as a potent solvent
for cellulose was patented already back in 1927 [88].
The transition from swelling to dissolution of cellulose in o-phosphoric acid, and its effect
on the cellulose accessibility to enzymatic hydrolysis, was studied by Zhang et al. and
dissolution took place without severe hydrolysis under the conditions used [89]. As often,
water plays a crucial role in the dissolution behavior of cellulose in this solvent. Phosphoric
acid is quite unique being triprotic and due to its ability to form dimers and even polymers.
The composition of the acid is usually expressed in P2O5-concentration, which, at a
concentration exceeding 74 % is anhydrous (superphosphoric acid). Thus, mixing different
species of phosphoric acids may give a powerful cellulose solvent, claimed to rapidly
dissolve up to 38 % w/w cellulose. This was shown recently and from these anisotropic
solutions of cellulose with a DP of 700-800 in water free phosphoric acid, spinning dopes for
production of textile fibers with only moderate chain degradation could be prepared. Fibers
can be spun by air gap spinning in acetone and neutralized by Na2CO3. The resulting yarn
showed extraordinary tenacity [90-91].
Carboxylic acids are not successfully used as direct dissolution media for cellulose. The
acidity is low, and the acids are likely to react with the cellulose, resulting in cellulose
derivatives that must be converted to pure cellulose in a second step. Acids in this group
that may be used as solvents for cellulose include trifluoroacetic acid, dichloroacetic acid
and formic acid with or without addition of sulfuric acid [92]. The dissolution is much faster
if sulfuric acid is used as a catalyst. However, the polymers dissolved in these acids are not

Direct Dissolution of Cellulose: Background, Means and Applications 159
cellulose but the corresponding cellulose derivative, meaning these are in fact not direct
solvents for cellulose but rather derivatizing solvents. Moreover, common for the cellulose
derivatives produced via this route is unsurprisingly that they show hydrolytic instability.
6.6. Non aqueous media
In 1934, Charles Graenacher reported the first non-aqueous direct solvent for cellulose in his
patent where he applied N-alkylpyridinium salts not only as fairly efficient dissolution
media but also for homogeneous reaction [93]. Since then a wide distribution of non
aqueous media for cellulose treatment have developed, for uses both within industry and
6.7. DMAc/LiCl
One of the most frequently used solvent systems for cellulose and other polysaccharides
when it comes to homogenous modification and analytical work such as size exclusion
chromatography is the dimethyl acetamide/lithium chloride mixture [94-96] first patented
by McCormic in the early 80s [97]. The DMAc/LiCl solvent system is very efficient and will
dissolve even high molecular weight cellulose like cotton linters or bacterial cellulose with
negligible chain degradation at moderate temperatures. Besides that, major advantages are
that the solutions are colorless and compatible with GPC columns.

Figure 9. Proposed interaction between solvent and polymer acting as the dissolution mechanism of the
system cellulose in DMAc/LiCl, adapted from McCormick, Callais et al. 1985.
The DMAc/LiCl solvent system for cellulose seems to be very specific in its interaction.
Neither DMAc with other lithium salts nor DMAc with other chloride salts seem to work in
the same way and not even the similar solvent dimethyl formamide with addition of LiCl.
The mechanism proposed by McCormick was that hydroxyl groups of cellulose interact
with a lithium DMAc macrocation via hydrogen bonding bridged by the chloride anion.
The lithium cation interacts with the carbonyl oxygen via ion dipole interaction [98]. For
this interaction to take place, no cellulose bound water can be present. As a consequence of
this, solvent exchange of the cellulose or distillation of the DMAc/LiCl/cellulose system is
often necessary. This is a time and solvent demanding step and is one reason why this
solvent system may not be preferred outside laboratory scale conditions. Moreover, the
solvent itself needs to be dry, since it is easily disturbed by water impurities [99]. Despite
early suggestions, the dissolution mechanism is disputed and still not completely
understood. However, the DMAc molecule is not able to form hydrogen bonds to any large

Cellulose Fundamental Aspects 160
extent, and that should leave the inorganic ions of the mixtures highly active and free to
interact with the polysaccharide instead [94].
This solvent system has been suggested for preparing regenerated cellulose. For example,
cellulose beads for support in columns used for liquid chromatography have successfully
been produced from DMAc/LiCl solutions and due to the efficiency of the solvent it was
possible to optimize the bead performance by adjusting DP and concentration of cellulose
[100]. DMAc/LiCl is also suitable for homogeneous derivatization of cellulose. As a true
solvent where no covalent bonding between solvent and solute takes place, the cellulose is
fully susceptible to reaction with other molecules [101-102].
In spite of its many uses, this solvent system experiences some problems. In size exclusion
chromatography, unwanted effects have been noticed [103] and under thermal stress the
cellulose solutions in DMAc/LiCl may fail to remain inert [104].
A new and very powerful solvent for cellulose consists of a mixture of dimethylsulfoxide
(DMSO) and tetrabutylammonium fluoride (TBAF). It is an efficient solvent and can
dissolve cellulose with a DP of up to 1200 within one hour at 60 C [105]. So far, the
DMSO/TBAF solvent system has been used mostly for analytical purposes e.g. NMR and
GPC but also for homogenous reactions for chemical modification of cellulose, both in lab-
scale only. Both etherifications and esterifications have been pursued with varying success

Figure 10. Dimethylsulfoxide (left) and tetrabutylammonium fluoride (right), here represented as
TBAF is a very hygroscopic salt and water content in DMSO/TBAF/cellulose solutions may
play a crucial role, depending on circumstances. Anhydrous TBAF is unstable [111-112] but
TBAF with water content up to the trihydrate are excellent cellulose solvents, while higher
water content is not tolerated. Other ammonium fluorides might be applicable as well [105].
6.9. N-Methylmorpholine-N-oxide: NMMO
Indisputable the most industrially successful of all non-derivatizing cellulose solvents is the
N-methylmorpholineN-oxide, commonly known as NMMO. Due to its ability to dissolve
high concentrations of cellulose directly and without alteration of the chemical properties of
the cellulose chain, as well as the possibility to recycle more than 99 % of the solvent after
usage, this particular solvent has proven to be a viable commercial solvent system. Fibers

Direct Dissolution of Cellulose: Background, Means and Applications 161
produced from this solvent also show exceptional mechanical and tactile properties, making
NMMO a serious competition to the old but incredibly successful solvent system NaOH/CS2
used in the viscose process. The NMMO solvent system has clear advantages in that it is
non-derivatizing, since many process steps may be left out.

Figure 11. N-methylmorpholine-N-oxide, a cyclic, aliphatic tertiary amine oxide.
NMMO is completely soluble in water, and as a pure substance it is extremely hygroscopic.
The high polarity of the N O bond also results in a pronounced ability to form hydrogen
bonds. There are two stable hydrates of NMMO, the monohydrate NMMO*H2O and the 2.5-
hydrate NMMO*2.5H2O. As cellulose solvent the NMMO*H2O is preferred, and cellulose is
soluble all the way up to NMMO*1.2H2O while for higher amounts of water precipitation of
cellulose takes place. The NMMO oxygen is able to form two hydrogen bonds with nearby
hydroxyl groups such in water or cellulose [113]. Hence, the monohydrate can dissolve
cellulose while the 2.5-hydrate cannot. The competition of water and cellulose for these
hydrogen bonds is the central feature of a whole industry based on dissolution and
regeneration of cellulose in the NMMO system.
The procedure for dissolving cellulose in NMMO usually includes a first step where a
suspension of cellulose in NMMO and a large excess of water is well mixed. The excess
water provides low viscosity and thereby superior mixing. Surplus water is then removed
by heat between 100 and 120 C and reduced pressure until the point of complete cellulose
dissolution is reached and the spinning dope is in its final form of approximately 14 %
cellulose, 10 % water and 76 % NMMO.
The dissolution process of cellulose in NMMO at elevated temperatures is performed
under strictly controlled conditions, since NMMO is thermally unstable. The N O bond
is energy rich and upon cleavage releases 222 kJ/mol. Furthermore, NMMO is an oxidant
and is sensitive to all forms of catalytic impurities in the dope. NMMO was first
considered as an unproblematic solvent without byproduct in the process of dissolving
cellulose, but as the knowledge of the possible side reactions in the cellulose/NMMO have
increased, the understanding of industrial challenges has improved [114-116]. Due to the
reactivity of NMMO and the severe degradation of cellulose that may be the result of this,
stabilizers must be added already in the beginning of the dissolution process. Such
stabilizers are for example propyl gallate, which is the most known. This compound
forms a strongly colored chromophore during usage, and other stabilizers are currently
being investigated [117]. The degradation of NMMO and subsequent side reactions may
include N-methylmorpholine, morpholine and formaldehyde. Some reactions are induced
by transition metal ions such as iron and copper, why these must be strictly excluded
from the process [118].

Cellulose Fundamental Aspects 162
To be able to prepare cellulose solutions in NMMO without stabilizers and without chain
degradation, temperature and/or time must be kept low and while this is not doable under
commercial conditions, stabilizers are a crucial part of the process. To succeed without
stabilizers, high shear must be applied throughout the dissolution and immediately
subsequent spinning process [119]. This accelerates the dissolution process and results in a
smooth dissolution without undissolved gel particles. Mechanical energy added to the
system might have the role of facilitating breakage of cellulose cellulose intermolecular
bonds, enabling solvent molecules to coordinate to the polymer instead. In addition, the
apparent viscosity decreases with shear stress due to cellulose alignment, as expected in
non-Newtonian polymer solutions. Viscosity is of course also dependent on cellulose
concentration and degree of polymerization of chosen substrate.
NMMO is now successfully applied as a non derivatizing cellulose solvent on industrial
scale, and a huge amount of research is available on the subtle details on the interactions
between polymers and solvent. For example, activation of cellulose seems to play a
significant role in the aggregation behavior of cellulose in the NMMO monohydrate solution
6.10. Ionic liquids
This broad class of solvents comprises low melting salts with an organic cation and an
organic or inorganic anion. Several comprehensive overviews of the field have been
published since 2002 when Swatloski found the neat imidazolium salt 1-butyl-3-methyl
imidazolium chloride (BMIMCl) to dissolve cellulose [121]. As previously mentioned,
Graenacher found already in 1934 that liquefied quaternary ammonium salts, alone or
diluted in suitable solvents, could dissolve cellulose. He worked mostly with pyridinium
chlorides, but at the time this did not attract the attention it might have deserved [93]. Ionic
liquids (IL) represent chemicals simply defined as organic salts with a melting point below
100 C and are not limited to being solvents. Room temperature ionic liquids (RTIL) are
often considered the second generation of ionic liquids. The possibility to pair anions with
cations yields an almost endless library of potential ionic liquids, and the possibilities to
adjust chemical and physical properties of the resulting salts are immense. Since the late
1990s, the interest in ionic liquids has grown fast. Ionic liquids have now penetrated many
areas of research and industrial applications of ionic liquids can be found in as wide spread
areas as pharmaceutics, analytical chemistry, separation and extraction, materials science
and as electrolytes in batteries. For cellulose applications the topic is an ever growing area of
research. This relatively new solvent class has already shown great versatility in the field of
cellulose technology, including dissolution for regeneration purposes [122-123],
homogeneous derivatization [124], and biomass processing including wood component
separation [125-127].
The ionic liquids that are able to dissolve cellulose include several classes of cations, and a
multitude of anions. Some of the most common cations are imidazolium, pyridinium,
ammonium and phosphonium derivatives, shown in Figure 12. The most popular cation

Direct Dissolution of Cellulose: Background, Means and Applications 163
used today is the imidazolium cation with different alkyl substituents. The effect of alkyl
chain length on the cellulose dissolution ability was acknowledged already 2002 by
Swatloski and has since then been observed by several groups [128-129]. Studies on the
imidazolium cation itself, not taking into account its properties as a solvent, recently
explained in detail the effect of cation symmetry and found effects on e.g. glass temperature,
viscosity and ion mobility [130]. The asymmetrical cation provides the ionic liquid with
lower viscosity, which indeed is an important feature for a solvent.

Figure 12. Technically relevant ionic liquid cations for cellulose processing.
The dissolution mechanism of cellulose in ionic liquids has long been argued to be all about
hydrogen bond interaction. Several studies have confirmed that the anion of the ionic liquid
penetrates the cellulose structure and dissembles the native cellulose structure by
competitive hydrogen bonding [131-133]. The anion acts as a hydrogen bond acceptor and
the cation as a hydrogen bond donor.
In his original article Swatloski argued that hydrogen bond capability, and therefore the
ability to dissolve cellulose, was directly related to the anion concentration in the close
proximity of the polymer. This was said to be the reason for the fact that the butyl
imidazolium salt but not the corresponding salts with higher alkyl chains could dissolve
cellulose. Smaller cations simply allow for more anions to crowd around the cellulose chain
[128]. The role of the cation in the dissolution mechanism is still disputed. Some simply
leave the cation out of the discussion while others attributes the cation a more prominent
role, e.g. as electron acceptor and hydrogen bond donor as according to Feng and Chen, c.f.
Figure 12. In that case, both the cation and the anion need to be small enough to reach the
hydroxyl groups of the cellulose, forming a electron donor electron acceptor complex,
break the polysaccharide polysaccharide interactions and finally solubilize the polymer
[134]. Proof of this is claimed to be the change in cellulose dissolution capability in
imidazolium salts when the acidic proton on C2 is replaced by a methyl group [123].
Viscosity and other rheological properties are always important when working with
polymer melts or solutions. One feature to examine is the Mark-Houwink parameters which
relate the molecular weight of the polymer with the intrinsic viscosity of the solution
according to the simple expression
|p] = KH
where [] is the intrinsic viscosity and M is the molecular weight if the polymer. K and are
the so called Mark-Houwink parameters. An indication of the polymer shape and indirectly

Cellulose Fundamental Aspects 164
a measure of the solvent quality can be acquired from these parameters. Cellulose solutions
in ionic liquids have been studied by Gericke and co-workers who examined the Mark-
Houwink parameters for cellulose/1-ethyl-3-methyl-imidazolium acetate (EMIMAc)
solutions, and report -values of 0.4 0.6 in the temperature range of 0 100 C. A value of
around 0.8 indicates a good solvent and in general, -values of 0.65 to ~1 have been
reported for other cellulose solutions [135].

Figure 13. Interaction between cellulose and imidazolium type ionic liquid, adapted from Feng and co
workers [134].
It has been shown numerous times that ionic liquids can be used as a reaction media for
homogeneous derivatization as well as for dissolution of cellulose. Further, it has been
proposed that due to their good dissolution properties, the use of ionic liquids can aid in
control of degree of substitution in for example acetylation and tosylation reactions using
various ionic liquids, reaction conditions and reactants [136-138]. Unlike in DMAc/LiCl
solutions, no catalyst seems to be needed. The degree of substitution be controlled and a
very wide range of DS can be achieved [136]. Silylations of cellulose in solutions of BMIMCl
and EMIMAc using hexamethyldisilazane as silylating agent with high yields were also
recently reported. Degree of substitution was controlled by reaction conditions and DS = 3
were achievable [139].
Ionic liquids as reaction media for homogeneous derivatization of cellulose turn out to be
efficient enough to possibly compete with todays heterogeneous reaction routes. With
optimization of reaction conditions the possibilities to control DS and possibly even
substitution pattern opens up for production of new materials based on cellulose. One of the
most important cellulose derivatives is still the cellulose acetate. Acetylation of cellulose can
be performed using the ionic liquid not only as a solvent but as the actual reagent [140]. This
was first discovered as an unexpected side reaction when in fact the goal was to react
cellulose with acid chlorides, trityl chlorides and tosyl chlorides. The resulting polymer was
acetylated, meaning that parts of the solvent, in this case the acetate anions of the ionic
liquid EMIMAc, are consumed. In large scale applications this might turn out to be a serious
problem, since recycling of the solvent requires that it does not degrade or react during the
process. Not only will the recycling be more complicated due to side products, it will also be
incomplete and in large scale application recycling of the solvent is necessary for both
economic and environmental sustainability [141].

Direct Dissolution of Cellulose: Background, Means and Applications 165
Mixed solvent systems provide further possibilities in cellulose processing. Adding a co-
solvent might serve as a means to lower the viscosity of the solution and thereby facilitate fast
dissolution rate and overall ease of handling. Mixtures of ionic liquids and organic solvents
includes BMIMCl in 1,3-dimethyl-2-imidazolidinone (DMI) that, thanks to its low viscosity
allows for efficient mixing and no agglomeration. This system is claimed to dissolve 10 %
cellulose (Avicel) within a few minutes at 100 C. This behavior is explained by the fact that
only a fraction of IL in the proper molecular solvent may shift the solvatochromic Kamlet Taft
parameters , and * to the point where cellulose is dissolved [142]. Remsing and co-
workers studied the molecular interactions of BMIMCl in water and DMSO, respectively, and
found that while water completely solvates the ions already at low concentrations high density
clusters of ionic liquid were found even at concentrations as low as 10 %. The poor interaction
between ions and DMSO thereby showed suggests that DMSO could be used as a rather inert
co-solvent, leaving the ionic liquid intact to act as solvent [143].
Recently an interest in so called switchable or distillable ionic liquids has risen. One
important reason for this is the need to recycle and also purify the ionic liquid after use. By
reintroducing molecular traits of the ionic liquid, i.e. turning it into a molecular solvent
(mixture), it may be possible to distill the components and thereby achieve high purities.
Thermal instability of the solvent may actually have a profound impact on the choice of
recycling techniques. The possibility of irreversible decomposition of the ionic liquid must
be taken into account, and there are several routes by which the e.g. the imidazolium cation
can decompose [144]. This concept may contradict the often assumed statements that ionic
liquids in general are both non-volatile and thermally stable. Imidazolium based ionic
liquids are known to decompose under temperatures exceeding 200 C and reduced
pressure. EMIMCl shows fragmentation into 1-methylimidazole, 1-ethylimidazole,
chloromethane and chloroethane [145]. The fragments obtained by thermolysis can be
further distilled and separated for later use as starting materials in a reaction to regenerate
the original ionic liquid [146]. Problems arise when the thermal decomposition occurs
unexpected or via unknown routes. Recent investigations have shown that common ionic
liquids such as EMIMCl and BMIMCl start to degrade already at temperatures around 120
C [144]. The free acids resulting from the decomposition may cause problems in
carbohydrate processing, since depolymerization can be expected.
In 2009, BASF patented a method for distillation of ionic liquids such as the cellulose solvent
EMIMAc. For a successful distillation, the ionic liquid must be converted into volatile
compounds, which upon condensation again form the wanted salt. The results were good, but
not outstanding, with a yield of > 90 % and a purity of > 95 % at 170 C [147]. The failure to
reach better results is most likely due to side reactions where unwanted decomposition occurs.
Recently, a new class of distillable tetramethylguanidine based ionic liquids was found to
dissolve cellulose. In this case, the ionic liquid will dissociate and form the volatile
corresponding acid and base pair [29]. Decreased temperature will again form the ionic
liquid, according to the equilibrium in Figure 14. In this case, the distillation can be very
successful and the reported yield and purity both exceeded 99 %.

Cellulose Fundamental Aspects 166

Figure 14. Distillation of a tetramethylguanidine based ionic liquid.
7. Cellulose solutions
Due to the strong tendency to self-aggregation in cellulose and the challenge of finding a
proper solvent, most industrial cellulose solutions are actually not true molecular solutions.
Knowledge of the character of the solution system is of importance for optimization of
industrial systems as well as in academia, e.g. for characterization of the polymer using size
exclusion chromatography. Comparisons between the cellulose structures in different solvents
may be performed using e.g. small angle X-ray scattering (SAXS), small angle light scattering
(SLS), dynamic light scattering (DLS) and depolarized dynamic light scattering (DDLS) [148].
Several different models of partially substituted cellulose in solution have been proposed.
By light scattering experiments it is possible to approximate the aggregation number, and
also the shape of the cellulose aggregates, if present. Models suggest star-branched
macromolecules or fringed micelles [149-150]. However, these models were based on
experiments using cellulose derivatives and even low degree of substitution may
significantly change the behavior of the polymer in a given solvent.
For high concentration cellulose solutions very different behavior can be observed.
Lyotropic mesophases or liquid crystalline phases in solutions of NMMO/cellulose are
thought to be possible if the concentration of cellulose exceeds 20 % [151-153]. Comparable
solutions can also be formed in an ammonia/ammonium thiocyanate solvent. The nature of
the solution can be controlled to selectively produce lyotropic cellulose mesophases having
either nematic or cholesteric character. High strength, high modulus fibers can be produced
from a solution of cellulose in the nematic phase. Films can be prepared from mesophase
solutions of cellulose that are mainly in the cholesteric phase. Material properties vary
significant with cellulose concentration [154]. Similar solutions for fiber spinning
applications have been prepared and solution anisotropy have been observed using
inorganic acids [155] and ionic liquids [121, 133, 156] at cellulose concentrations over 10 %.
For DMAc/LiCl solutions of cellulose, the polymer concentrations needs to exceed 15 % [98].
The ordered structure can be induced not only in the liquid state but of course also in the gel
like state. Nematic ordered films were prepared from molecularly dispersed DMAc/LiCl
solutions by stretching of a water swollen gel phase attained by slow coagulation in water
vapor [157]. Still, the mechanisms of liquid crystalline phase transition and gelsol transition
are not yet fully understood and much research is left to do. The question must be raised
whether to actually define liquid crystalline phases as true solutions when obvious
anisotropy is present.

Direct Dissolution of Cellulose: Background, Means and Applications 167
8. Conclusions
The development and optimization of efficient cellulose solvents have had tremendous
impact on cellulose processing for well over a hundred years, and will continue to be
important as the interest in cellulose itself as a basis for new materials and other
applications is increasing. The most important question when considering the dissolution of
cellulose is what the purpose of keeping the cellulose in solution will be. For homogeneous
reactions in small scale the requirements on the solvents may be completely different from
those in a large scale industrial facility for dissolution and shaping of cellulose where the
purpose of regeneration is not only to stabilize the physical structure but also regain the
original chemical composition of the polymer. Degree of polymerization, source and purity
of the cellulose as well as required concentration in the solution may also have a profound
effect on the choice of solvent.
A wide variety of suitable solvents for cellulose are already available, both aqueous and
non-aqueous. Despite this, feverish activity goes on in both industry and academia to
optimize existing solvent systems and develop new ones. Ideally, such a new cellulose
solvent would of course be efficient and inert towards the polymer itself. It would also be
100 % recyclable and non-hazardous to environment and man. To meet future requirements
on green chemistry and sustainability, it would be produced from non-hazardous starting
materials and upon final degradation give non-hazardous, biodegradable products only.
Proper life cycle assessment could be conducted to see if any new solvent meets these
New materials produced from cellulose raw material are claimed to fall under the category
of sustainable development because the cellulose is a polymer that replenishes itself in
plants by air, water and solar energy. However, the chemistry we apply to modify and
shape the cellulose to whatever form we desire must also be considered. Solvents are no
exception, especially not since they are often used in great excess meaning large quantities.
Truly sustainable polymer based materials are only achieved by using a sustainable polymer
in combination with a sustainable chemistry to finalize the product. The quest for the
cellulose solvent of the future goes on.
Author details
Carina Olsson
Corresponding Author
Department of Chemical and Biological Engineering, Chalmers University of Technology,
Gothenburg, Sweden
Gunnar Westman
Department of Chemical and Biological Engineering, Chalmers University of Technology,
Gothenburg, Sweden

Cellulose Fundamental Aspects 168
The authors would like to acknowledge Prof. Herbert Sixta and Dr. Merima Hasani for
valuable discussions and critical reading of the manuscript. Many thanks also to Sdra Cell
for financial support.
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