RT Journal Article SR Electronic T1 Detection of cell-affecting agents with a silicon biosensor JF Science JO Science FD American Association for the Advancement of Science SP 243 OP 247 DO 10.1126/science.2799384 VO 246 IS 4927 A1 Parce, JW A1 Owicki, JC A1 Kercso, KM A1 Sigal, GB A1 Wada, HG A1 Muir, VC A1 Bousse, LJ A1 Ross, KL A1 Sikic, BI A1 McConnell, HM YR 1989 UL http://science.sciencemag.org/content/246/4927/243.abstract AB Cellular metabolism is affected by many factors in a cell's environment. Given a sufficiently sensitive method for measuring cellular metabolic rates, it should be possible to detect a wide variety of chemical and physical stimuli. A biosensor has been constructed in which living cells are confined to a flow chamber in which a potentiometric sensor continually measures the rate of production of acidic metabolites. Exploratory studies demonstrate several applications of the device in basic science and technology.