@article {Zulianello3134, author = {Zulianello, Laurence and Canard, Coralie and K{\"o}hler, Thilo and Caille, Doroth{\'e}e and Lacroix, Jean-Silvain and Meda, Paolo}, title = {Rhamnolipids Are Virulence Factors That Promote Early Infiltration of Primary Human Airway Epithelia by Pseudomonas aeruginosa}, volume = {74}, number = {6}, pages = {3134--3147}, year = {2006}, doi = {10.1128/IAI.01772-05}, publisher = {American Society for Microbiology Journals}, abstract = {The opportunistic bacterium Pseudomonas aeruginosa causes chronic respiratory infections in cystic fibrosis and immunocompromised individuals. Bacterial adherence to the basolateral domain of the host cells and internalization are thought to participate in P. aeruginosa pathogenicity. However, the mechanism by which the pathogen initially modulates the paracellular permeability of polarized respiratory epithelia remains to be understood. To investigate this mechanism, we have searched for virulence factors secreted by P. aeruginosa that affect the structure of human airway epithelium in the early stages of infection. We have found that only bacterial strains secreting rhamnolipids were efficient in modulating the barrier function of an in vitro-reconstituted human respiratory epithelium, irrespective of their release of elastase and lipopolysaccharide. In contrast to previous reports, we document that P. aeruginosa was not internalized by epithelial cells. We further report that purified rhamnolipids, applied on the surfaces of the epithelia, were sufficient to functionally disrupt the epithelia and to promote the paracellular invasion of rhamnolipid-deficient P. aeruginosa. The mechanism involves the incorporation of rhamnolipids within the host cell membrane, leading to tight-junction alterations. The study provides direct evidence for a hitherto unknown mechanism whereby the junction-dependent barrier of the respiratory epithelium is selectively altered by rhamnolipids.}, issn = {0019-9567}, URL = {https://iai.asm.org/content/74/6/3134}, eprint = {https://iai.asm.org/content/74/6/3134.full.pdf}, journal = {Infection and Immunity} }