TY - JOUR T1 - Apoptosis: Calling Time on Apoptosome Activity JF - Science Signaling JO - Sci. Signal. SP - pe62 LP - pe62 DO - 10.1126/scisignal.291pe62 VL - 2 IS - 91 AU - Adrain, Colin AU - Martin, Seamus J. Y1 - 2009/10/06 UR - http://stke.sciencemag.org/content/2/91/pe62.abstract N2 - Apoptosis is a controlled form of cellular demolition, catalyzed by a family of cysteine proteases called caspases. In response to diverse proapoptotic stimuli, caspase-9 is recruited and activated within an oligomeric complex called the apoptosome. The apoptosome drives autocatalytic processing of caspase-9, triggering a proteolytic caspase cascade that results in the biochemical and morphological changes characteristic of cell death. It is unclear why caspase-9 undergoes autocatalytic processing following apoptosome recruitment, because interdomain processing is dispensable for caspase-9 activity. A study has shed light on this issue by demonstrating that caspase-9 processing within the apoptosome promotes its displacement from the complex, leading to inactivation of this protease. Thus, autoprocessing of caspase-9 within the apoptosome serves as a “molecular timer” that limits the proteolytic activity of this complex through displacement of bound caspase-9 molecules. This timer mechanism may enable cells to prevent low amounts of apoptosome activation from spiraling out of control unless sufficient numbers of apoptosomes are assembled within a particular time window, which would drive full-blown caspase activation and apoptosis. ER -