LY294002, wortmannin and rapamycin attenuates flagellin-induced cell proliferation. Wenlei Bao Yanfeng Wang Yuting Fu Xiaoyang Jia Jiaxin Li Nyamtsengel Vangan Lili Bao Huifang Hao Zhigang Wang 10.1371/journal.pone.0125910.g003 <p>Ana-1 cells were pretreated with LY294002 (10 μM), wortmannin (10 nM) and rapamycin (100 nM) for 4 h and then stimulated with 100 ng/ml flagellin for 24 h, and cell proliferation was measured by methyl thiazolyl tetrazolium (MTT) assay. A. Proliferation was inhibited by LY294002 and wortmannin in response to flagellin. B. Proliferation was inhibited by rapamycin in response to flagellin. Experiments were made in triplicate, and data are representative of three separate experiments (mean ± SD). *<i>p</i>< 0.05 compare to untreated controls, # <i>p</i>< 0.05 compared with flagellin group.</p> 2015-05-05 03:11:57 STAT 3 flagellin rapamycin PI 3K phosphorylation PI 3K pathway tnf expression TLR 5. Inhibition nf PI 3K mTORC 1 ly cytokine il response