Goat Anti-Rabbit IgG, FITC-labeled [FL-04]


SKU: FL-04 Category: Product Size: 500 ug | Antibody Type: affinity-purified | Host: Goat | Reactivity: rabbit | Conjugate: FITC | Usage: flow cytometry |


FITC-labeled goat anti-rabbit IgG can be used to verify specific binding of rabbit IgG to its receptor. By first incubating cells with the primary rabbit antibody, and then binding the FITC-labeled goat anti-rabbit IgG to the primary antibody, a fluorescent marker is formed that can demonstrate expression of a receptor or affinity of an antibody for its receptor. FITC is excited by 488 nm wavelength light, and emits at 525 nm.

This fluorescent conjugate was prepared using goat anti-rabbit IgG (H+L) and the fluorescent compound, fluorescein isothiocyanate (FITC). The antibody binds to rabbit IgG, and is affinity-purified to decrease background and non-specific binding. This antibody exhibits maximal binding to rabbit IgG antibodies, and minimal cross-reactivity with other molecules.

Applications include flow cytometry.


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