A: Well, HUge, you would've had a lot more laughs driving the choo-choo (laughs).

B: (Stares with barely restrained contempt at the obvious incarnation of a chimp into human form).

A: I'm just busting your balls, Huge.

B: (To his servitor) Call in our debt and take me from the presence of this small dog.

What does that mean?

Asking that a debt/money owned to you is paid up.
To demand/require that a debt is paid off to you.

I've no idea what this means in this context.
 Marius Hancu's reply was promoted to an answer.