Material Data Sheet

SUMO3 Conjugation Kit

Price: $355.00
Catalog #: K-720

SUMO3 Conjugation Kit

1 Kit
Data Sheet: 

This kit is designed for the conjugation of the Ubiquitin-like modifier SUMO3 to protein substrates in vitro, which requires the activities of the heterodimeric human E1 activating enzyme (SAE1/SAE2) and the Ubc9 E2 enzyme.  This kit does not contain a SUMO E3 ligase, which may be needed effective SUMOylation of some proteins.


Product Information

Western Blot Data: 
Western Blot Data: 
The Ubiquitin-like SUMO3 is conjugated to a variety of proteins in the presence of Ubc9 and the SUMO E1 activating enzyme (SAE1/SAE2 in human, or Aos1/Uba2p in yeast). This isoform is known to form poly-SUMO chains on substrates via isopeptide bond formation between the C-terminal glycine of one SUMO3 to the lysine-ε-amino group of K11 on the preceding SUMO3. The heterodimeric SAE1/SAE2 complex (38 and 70 kDa respectively) uses ATP to adenylate the C-terminal glycine residue of SUMO3, forming a thioester bond with the SAE2 subunit. The second step is the trans-esterification reaction whereby the activated SUMO3 is transferred to Cys93 of Ubc9. Ubc9 is a member of the E2 family and is homologous to Ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it sometimes interacts directly with protein substrates that are modified by SUMOylation, and may play a role in substrate recognition. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates(IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein function and stability.
