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Université de Fribourg

Secreted venom allergen-like proteins of helminths: Conserved modulators of host responses in animals and plants

Wilbers, Ruud H. P. ; Schneiter, Roger ; Holterman, Martijn H. M. ; Drurey, Claire ; Smant, Geert ; Asojo, Oluwatoyin A. ; Maizels, Rick M. ; Lozano-Torres, Jose L.

In: PLOS Pathogens, 2018, vol. 14, no. 10, p. e1007300

Despite causing considerable damage to host tissue at the onset of parasitism, invasive helminths establish remarkably persistent infections in both animals and plants. Secretions released by these obligate parasites during host invasion are thought to be crucial for their persistence in infection. Helminth secretions are complex mixtures of molecules, most of which have unknown molecular...

Université de Fribourg

Localization and functional characterization of the pathogenesis-related proteins Rbe1p and Rbt4p in Candida albicans

Bantel, annick ; Darwiche, Rabih ; Rupp, Steffen ; Schneiter, Roger ; Sohn, Kai

In: PLOS ONE, 2018, vol. 13, no. 8, p. e0201932

Members of the Cysteine-rich secretory protein, Antigen 5 and Pathogenesis-related 1 (CAP) protein superfamily are important virulence factors in fungi but remain poorly characterized on molecular level. Here, we investigate the cellular localization and molecular function of Rbe1p and Rbt4p, two CAP family members from the human pathogen Candida albicans. We unexpectedly found that Rbe1p...

Consortium of Swiss Academic Libraries

The role of lipids in the biogenesis of integral membrane proteins

Schneiter, Roger ; Toulmay, Alexandre

In: Applied Microbiology and Biotechnology, 2007, vol. 73, no. 6, p. 1224-1232

Université de Fribourg

Heligmosomoides polygyrus venom allergen-like protein-4 (HpVAL-4) is a sterol binding protein

Asojo, Oluwatoyin A. ; Darwich, Rabih ; Gebremedhin, Selam ; Smant, Geert ; Lozano-Torres, José L. ; Drurey, Claire ; Pollet, Jeroen ; Maizels, Rick M. ; Schneiter, Roger ; Wilbers, Ruud H. P.

In: International Journal for Parasitology, 2018, vol. 48, no. 5, p. 359–369

Heligmosomoides polygyrus bakeri is a model parasitic hookworm used to study animal and human helminth diseases. During infection, the parasite releases excretory/secretory products that modulate the immune system of the host. The most abundant protein family in excretory/secretory products comprises the venom allergen-like proteins (VALs), which are members of the SCP/TAPS (sperm-coating ...

Université de Fribourg

The function of yeast CAP family proteins in lipid export, mating, and pathogen defense

Darwiche, Rabih ; Atab, Ola El ; Cottier, Stéphanie ; Schneiter, Roger

In: FEBS Letters, 2018, vol. 592, no. 8, p. 1304–1311

In their natural habitat, yeast cells are constantly challenged by changing environmental conditions and a fierce competition for limiting resources. To thrive under such conditions, cells need to adapt and divide quickly, and be able to neutralize the toxic compounds secreted by their neighbors. Proteins like the pathogen‐related yeast, Pry proteins, which belong to the large CAP/SCP/TAPS ...

Université de Fribourg

Crystal structure of Brugia malayi venom allergen-like protein-1 (BmVAL-1), a vaccine candidate for lymphatic filariasis

Darwiche, Rabih ; Lugo, Fernanda ; Drurey, Claire ; Varossieau, Koen ; Smant, Geert ; Wilbers, Ruud H. P. ; Maizels, Rick M. ; Schneiter, Roger ; Asojo, Oluwatoyin A.

In: International Journal for Parasitology, 2018, vol. 48, no. 5, p. 371–378

Brugia malayi is a causative agent of lymphatic filariasis, a major tropical disease. The infective L3 parasite stage releases immunomodulatory proteins including the venom allergen-like proteins (VALs), which are members of the SCP/TAPS (Sperm-coating protein/Tpx/antigen 5/pathogenesis related-1/Sc7) superfamily. BmVAL-1 is a major target of host immunity with >90% of infected B. malayi...

Université de Fribourg

Architecture of lipid droplets in endoplasmic reticulum is determined by phospholipid intrinsic curvature

Choudhary, Vineet ; Golani, Gonen ; Joshi, Amit S. ; Cottier, Stéphanie ; Schneiter, Roger ; Prinz, William A. ; Kozlov, Michael M.

In: Current Biology, 2018, vol. 28, no. 6, p. 915-926.e9

Lipid droplets (LDs) store fats and play critical roles in lipid and energy homeostasis. They form between the leaflets of the endoplasmic reticulum (ER) membrane and consist of a neutral lipid core wrapped in a phospholipid monolayer with proteins. Two types of ER-LD architecture are thought to exist and be essential for LD functioning. Maturing LDs either emerge from the ER into the...

Université de Fribourg

Sphingolipid accumulation causes mitochondrial dysregulation and cell death

Knupp, Jeffrey ; Martinez-Monta?és, Fernando ; Van Den Bergh, Francoise ; Cottier, Stephanie ; Schneiter, Roger ; Beard, Daniel ; Chang, Amy

In: Cell Death and Differentiation, 2017, vol. 24, no. 12, p. 2044

Sphingolipids are structural components of cell membranes that have signaling roles to regulate many activities, including mitochondrial function and cell death. Sphingolipid metabolism is integrated with numerous metabolic networks, and dysregulated sphingolipid metabolism is associated with disease. Here, we describe a monogenic yeast model for sphingolipid accumulation. A csg2Δ mutant...

Université de Fribourg

Crystal Structure of Borrelia turicatae protein, BTA121, a differentially regulated gene in the tick-mammalian transmission cycle of relapsing fever spirochetes

Luo, Zhipu ; Kelleher, Alan J. ; Darwiche, Rabih ; Hudspeth, Elissa M. ; Shittu, Oluwatosin K. ; Krishnavajhala, Aparna ; Schneiter, Roger ; Lopez, Job E. ; Asojo, Oluwatoyin A.

In: Scientific Reports, 2017, vol. 7, no. 1, p. 15310

Tick-borne relapsing fever (RF) borreliosis is a neglected disease that is often misdiagnosed. RF species circulating in the United States include Borrelia turicatae, which is transmitted by argasid ticks. Environmental adaptation by RF Borrelia is poorly understood, however our previous studies indicated differential regulation of B. turicatae genes localized on the 150 kb linear...

Université de Fribourg

Chemical crosslinking and mass spectrometry to elucidate the topology of integral membrane proteins

Debelyy, Mykhaylo O. ; Waridel, Patrice ; Quadroni, Manfredo ; Schneiter, Roger ; Conzelmann, Andreas

In: PLOS ONE, 2017, vol. 12, no. 10, p. e0186840

Here we made an attempt to obtain partial structural information on the topology of multispan integral membrane proteins of yeast by isolating organellar membranes, removing peripheral membrane proteins at pH 11.5 and introducing chemical crosslinks between vicinal amino acids either using homo- or hetero-bifunctional crosslinkers. Proteins were digested with specific proteases and the...