RT Journal Article SR Electronic(1) A1 Christopher, W. L. A1 Adler, B. A1 Faine, S. YR 1982 T1 Immunogenicity of Leptospiral Vaccines Grown in Protein-free Medium JF Journal of Medical Microbiology, VO 15 IS 4 SP 493 OP 501 DO https://doi.org/10.1099/00222615-15-4-493 PB Microbiology Society, SN 0022-2615, AB Summary Leptospira interrogans serovars pomona and hardjo were adapted to grow in a chemically-defined, protein-free (PF) medium. Formolised monovalent vaccines of serovars pomona and hardjo and a bivalent mixture of the two were prepared from PF cultures. Live PF cultures and the vaccine preparations retained their agglutinating antigens and their immunogenicity when tested in rabbits and guineapigs. The vaccines were not pyrogenic and dermal reactions were slight., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-15-4-493