Veratox® for Florfenicol is a competitive enzyme immunoassay for the quantitative analysis of florfenicol in salmon. Veratox for Florfenicol enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect florfenicol in salmon muscle in response to customer concerns about food safety. Veratox for Florfenicol is an accurate, rapid, cost-effective and easy-to-use diagnostic tool compared to instrument based methods like HPLC and GC.
Analyte Florfenicol
Brand Veratox®
Cross Reactivity Florfenicol 100%
Limit of Detection 150 ppb
Platform ELISA
Quantity per Package 36 tests
Range of Quantitation 0.15 ppm - 100.00 ppm
Result Type Quantitative
Testing Time 60.00 - 90.00 Minutes
Time to Result 60 - 90 Minutes

  1. Florfenicol-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips)
  2. Florfenicol Standards:
    1. Negative control (white cap tube) 0.8 mL
    2. 0.15 ng/mL (yellow cap tube) 0.8 mL
    3. 1.0 ng/mL (orange cap tube) 0.8 mL
    4. 5.0 ng/mL (pink cap tube) 0.8 mL
    5. 25.0 ng/mL (purple cap tube) 0.8 mL
    6. 100.0 ng/mL (blue cap tube) 0.8 mL
    7. 20,000.0 ng/mL (spiking, red cap tube) 0.8 mL
  3. Florfenicol Antibody #1 12 mL
  4. 100X HRP-Conjugated Antibody #2 250 µL
  5. Antibody #2 Diluent 20 mL
  6. 10X Sample Extraction Buffer 25 mL
  7. 20X Wash Solution 28 mL
  8. Stop Buffer 14 mL
  9. TMB Substrate 12 mL
  2. 20–200 µL pipettes (Neogen item 9276)
  4. 1000 µL pipettor (Neogen 9337)
  5. Vortex Mixer (Neogen 9494)
  6. Centrifuge 4,000 x g
  7. Timer (Neogen item 9426)
  8. Wash bottle (Neogen item 9400)
  9. Paper towels or equivalent absorbent material
  10. Veratox software (Neogen item 9305)
  11. Lab station (Neogen item 9481)
  12. Distilled or deionized water
  13. Blender or food processor to homogenize sample





