PT - JOURNAL ARTICLE AU - Gou, Mingyue AU - Hou, Guichuan AU - Yang, Huijun AU - Zhang, Xuebin AU - Cai, Yuanheng AU - Kai, Guoyin AU - Liu, Chang-Jun TI - The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis AID - 10.1104/pp.16.01614 DP - 2017 Feb 01 TA - Plant Physiology PG - 1045--1058 VI - 173 IP - 2 4099 - 4100 - SO - Plant Physiol.2017 Feb 01; 173 AB - Suberin, a lipophilic polymer deposited in the outer integument of the Arabidopsis (Arabidopsis thaliana) seed coat, represents an essential sealing component controlling water and solute movement and protecting seed from pathogenic infection. Although many genes responsible for suberin synthesis are identified, the regulatory components controlling its biosynthesis have not been definitively determined. Here, we show that the Arabidopsis MYB107 transcription factor acts as a positive regulator controlling suberin biosynthetic gene expression in the seed coat. MYB107 coexpresses with suberin biosynthetic genes in a temporal manner during seed development. Disrupting MYB107 particularly suppresses the expression of genes involved in suberin but not cutin biosynthesis, lowers seed coat suberin accumulation, alters suberin lamellar structure, and consequently renders higher seed coat permeability and susceptibility to abiotic stresses. Furthermore, MYB107 directly binds to the promoters of suberin biosynthetic genes, verifying its primary role in regulating their expression. Identifying MYB107 as a positive regulator for seed coat suberin synthesis offers a basis for discovering the potential transcriptional network behind one of the most abundant lipid-based polymers in nature.GlossaryTFtranscription factorqRTquantitative real-timeCol-0Columbia-0MSMurashige and SkoogGC-MSgas chromatography-mass spectrometrySEMscanning electron microscopyTEMtransmission electron microscopyRNA-seqRNA sequencingDAFdays after floweringY1Hyeast one-hybridChIPchromatin immunoprecipitationX-Gal5-bromo-4-chloro-3-indolyl β-d-galactopyranosideqPCRquantitative PCR