@article {Liu247, author = {Liu, Sin-Chieh and Kowalski, Stanley P. and Lan, Tien-Hung and Feldmann, Kenneth A. and Paterson, Andrew H.}, title = {Genome-Wide High-Resolution Mapping by Recurrent Intermating Using Arabidopsis thaliana as a Model}, volume = {142}, number = {1}, pages = {247--258}, year = {1996}, publisher = {Genetics}, abstract = {We demonstrate a method for developing populations suitable for genome-wide high-resolution genetic linkage mapping, by recurrent intermating among F2 individuals derived from crosses between homozygous parents. Comparison of intermated progenies to F2 and {\textquotedblleft}recombinant inbred{\textquotedblright} (RI) populations from the same pedigree corroborate theoretical expectations that progenies intermated for four generations harbor about threefold more information for estimating recombination fraction between closely linked markers than either RI-selfed or F2 individuals (which are, in fact, equivalent in this regard). Although intermated populations are heterozygous, homozygous {\textquotedblleft}intermated recombinant inbred{\textquotedblright} (IRI) populations can readily be generated, combining additional information afforded by intermating with the permanence of RI populations. Intermated populations permit fine-mapping of genetic markers throughout a genome, helping to bridge the gap between genetic map resolution and the DNA-carrying capacity of modern cloning vectors, thus facilitating merger of genetic and physical maps. Intermating can also facilitate high-resolution mapping of genes and QTLs, accelerating map-based cloning. Finally, intermated populations will facilitate investigation of other fundamental genetic questions requiring a genome-wide high-resolution analysis, such as comparative mapping of distantly related species, and the genetic basis of heterosis.}, issn = {0016-6731}, URL = {https://www.genetics.org/content/142/1/247}, eprint = {https://www.genetics.org/content/142/1/247.full.pdf}, journal = {Genetics} }