Veratox® for Lincomycin is used for the quantitative analysis of lincomycin in muscle. Veratox for Lincomycin enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect lincomycin in muscle in response to customer concerns about food safety.
Analyte Lincomycin
Brand Veratox®
Platform ELISA
Quantity per Package 36 tests
Range of Detection 7.00 ppb - 262.00 ppb
Result Type Quantitative
Testing Time 60.00 - 120.00 Minutes
Time to Result 50 - 55 Minutes

  1. Lincomycin Ab-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips)
  2. Lincomycin Standards:
    1. Nevative control (white cap tube) 1.8 mL
    2. 0.1 ng/mL (yellow cap tube) 1.8 mL
    3. 0.25 ng/mL (orange cap tube) 1.8 mL
    4. 0.6 ng/mL (pink cap tube) 1.8 mL
    5. 1.5 ng/mL (purple cap tube) 1.8 mL
    6. 3.75 ng/mL (blue cap tube) 1.8 mL
    7. 1,000 ng/mL (spiking, red cap tube) 1.8 mL
  3. Lincomycin-HRP Conjugate 6 mL
  4. 200X Sample Extraction Buffer 1 mL
  5. 20X Wash Solution 28 mL
  6. Stop Buffer 14 mL
  7. TMB Substrate 12 mL
  8. Sample Balance Buffer 0.5 mL
  9. 10X Lincomycin Dilution Buffer 6 mL
  1. Microtiter plate reader with a 450 nm filter (Neogen® item 9303)
  2. 20–200 µL pipettes (Neogen item 9276)
  3. Multichannel pipette: 50–300 µL (optional) (Neogen item 9385)
  4. 1000 µL pipettor (Neogen 9337)
  5. Vortex Mixer (neogen item 9494)
  6. Centrifuge 4,000 x g
  7. Timer (Neogen item 9426)
  8. Wash bottle (Neogen item 9400)
  9. Paper towels or equivalent absorbent material
  10. Veratox software (Neogen item 9305)
  11. Lab station (Neogen item 9481)
  12. Distilled or deionized water
  13. Blender or food processor to homogenize sample
  14. Heater block or incubator capable of at least 70°C

The method is based on a competitive colorimetric ELISA assay. Lincomycin, the drug of interest, has been coated in the plate wells. During the analysis, sample is added along with the lincomycin-horseradish peroxidase (Lincomycin-HRP) conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the target concentration in the sample.




