Frozen zoo

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A frozen zoo is a storage facility in which genetic materials taken from animals (e.g. DNA, sperm, eggs, embryos and live tissue) are gathered and thereafter stored at very low temperatures for optimal preservation over a long period (see cryopreservation). Some facilities also collect and cryopreserve plant material (usually seeds).

Zoos such as the San Diego Zoo[1] and research programs such as the Audubon Center for Research of Endangered Species[2][3] cryopreserve genetic material in order to protect the diversity of the gene pool of endangered species, or to provide for a prospective reintroduction of such extinct species as the Tasmanian Tiger[4] and the Mammoth.[5]

Frozen Zoo at San Diego Zoo Conservation Research has been freezing biological materials from animals and plants in liquid nitrogen (−196 °C) since 1976.[6] They currently store a collection of 8,400 samples from over 800 species and subspecies.[7] Frozen Zoo at San Diego Zoo Conservation Research has acted as a forbearer to similar projects at other zoos in the United States and Europe including the Frozen Ark Project.[8][9] However, there are still less than a dozen frozen zoos worldwide.[3]

At the United Arab Emirates Breeding Centre for Endangered Arabian Wildlife (BCEAW), Sharjah, the embryos stored include the extremely endangered Gordon’s wildcat (Felis silvestris gordoni) and the Arabian leopard (Panthera pardus nimr) (of which there are only 50 in the wild).[10]

Creating a frozen zoo[edit]

Gathering material for a frozen zoo is rendered simple by the abundance of sperm in males. Sperm can be taken from an animal following death. The production of eggs, which in females is usually low, can be increased through hormone treatment to obtain 10–20 oocytes, dependant on species. Some frozen zoos prefer to fertilize eggs and freeze the resulting embryo, as embryos are more resilient under the cryopreservation process.[10] The zoo also collects skin cell samples of endangered animals or extinct species. Scripps Research Center has successfully made them in to a cultures of special cells,induced pluripotent stem (IPS) cells. Now, theoretically, they are able to make sperm and egg cells with old skin cells.

The future of frozen genetic material[edit]

Stored material can be stored indefinitely[3] and used for artificial insemination, in vitro fertilisation, embryo transfer and cloning.

Artificial insemination provides a remedy for animals who, due to anatomical or physiological reasons, are unable to reproduce in the natural way. Reproduction of stored genetic material also allows for the fostering of genetic improvements, and the prevention of inbreeding.

Modern technology allows for genetic manipulation in animals without keeping them in captivity. However, the success of their restoration into the wild would require the application of new science and a sufficient amount of previously collected material.[10]

See also[edit]


  1. ^ Dan Collins (2002-10-14). "San Diego's Frozen Zoo". The Associated Press and CBS News. Retrieved 2010-04-28. 
  2. ^ "Species Survival Center". Audubon Nature Institute. Retrieved 2010-04-28. 
  3. ^ a b c "Frozen Zoo". Audubon Nature Institute. Retrieved 2010-04-28. 
  4. ^ Margit Kossobudzka (2002-10-14). "Wyginął, a teraz powraca". Gazeta Retrieved 2010-04-26. 
  5. ^ Jan Sochaczewski (2007-10-12). "Zamrożone mamuty powrócą do świata żywych". Retrieved 2011-02-20. 
  6. ^ Magdalena Pecul (December 1997). "ZAMROŻONE ZOO". „Wiedza i Życie”. Retrieved 2010-04-26. 
  7. ^ "Chill Out: Frozen Zoo Aiding Conservation Projects". San Diego Zoo. Retrieved 2010-04-26. 
  8. ^ "Scientific Meeting - The Frozen Ark Project". ZSL London Zoo. Retrieved 2010-04-26. 
  9. ^ "Welcome to the Frozen Ark". The Frozen Ark Project. Retrieved 2010-04-26. 
  10. ^ a b c F.J. de Haas van Dorsser MA VetMB MRCVS. "The Frozen Zoo: Breeding Centre for Endangered Arabian Wildlife, Sharjah, UAE". Arabian Wildlife. Retrieved 2010-04-26.