@article {Blackburne:2007-11-01T00:00:00:1061-4303:2437, author = "Blackburne, Richard and Vadivelu, Vel M and Yuan, Zhiguo and Keller, Jürg", title = "Determination of Growth Rate and Yield of Nitrifying Bacteria by Measuring Carbon Dioxide Uptake Rate", journal = "Water Environment Research", volume = "79", number = "12", year = "2007-11-01T00:00:00", abstract = "Nitrifier growth parameters—the maximum growth rate (μ Amax ) and yield ( Y A )—were estimated by measuring the rate of carbon dioxide uptake and additional rates of oxygen uptake and ammonia (or nitrite) use. Batch tests in a combined titrimetric and offgas analyzer with enriched Nitrobacter and Nitrosomonas cultures and an activated sludge sample were performed. The measured μ Amax values for the Nitrobacter and Nitrosomonas cultures were 0.67 ± 0.03 day −1 and 0.54 ± 0.09 day −1, while the Y A values were 0.072 ± 0.01 g volatile suspended solids (VSS) · g nitrogen (N) −1 and 0.14 ± 0.02 gVSS · gN −1, respectively. For the activated sludge sample, μ Amax was observed to increase with pH (μ Amax = 0.72 · 3.3 (pH-7.1)) over the range 6.8 to 7.1. All μ Amax and Y A values determined by this method were similar to those previously reported. Compared with other μ Amax and Y A estimation methods, this method allows for unique μ Amax and Y A estimations for given conditions from a single experiment.", pages = "2437-2445", url = "http://www.ingentaconnect.com/content/wef/wer/2007/00000079/00000012/art00010", doi = "doi:10.2175/106143007X212139", keyword = "growth rate, nitrifiers, carbon dioxide uptake, activated sludge, Nitrosomonas, Nitrobacter, yield, nitrification" }