CH391L/S13/Algal Biofuels

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Advantages over other Biofuels

Microalgae tend to have better photosynthetic efficiency than terrestrial plants, including corn and soybeans, the other major sources for biofuels[1]. Sugarcane, a major source of bioethanol, has a photosynthetic efficiency (that can reach 8% under optimal conditions, although it is more typically around 1%[2]. However, microalgae are far better at producing biomass per unit land, as they can produce up to 158 tonnes/hectare, compared to 75 tonnes/hectare for sugarcane[3]. In addition, microalgal dry mass can be up to 55% lipid, which is most relevant to biofuel production and is far greater than what can be managed by terrestrial plants[4].

When it comes to biodiesel production, microalgae greatly outdo their competitors. Biodiesels are defined as methyl- or ethyl- esters of 10-14 carbon fatty acids. Some other sources, such as soybeans and oilpalms, can reach barely 5% lipid dry mass under highly optimized conditions[3]. Microalgae can, even at a modest 15% lipid dry mass, would require only 10.8 million hectares (or 6% of US agricultural land) to produce the 530 million m3 of diesel consumed each year at present. Oil palms, in comparison, would require 61% of US agricultural land to produce the same amount. Sugarcane derived bioethanol, with 64% of the energy content of biodiesel, would require a similar amount of land to produce an amount of energy equivalent to 530 million m3 of diesel[3].


A Raceway Pond aquaculture. This method of aquaculture allows for good mixing and light exposure at low cost, although gas exchange, temperature control and sterility are poor[5].

One of the most distinct advantages of microalgae over other photosynthetic organisms is their tolerance and/or preference for marginal water sources[6]. This means that algal biofuels won't have to compete for resources like fresh water and arable land with food crops, unlike biofuels derived from corn and soybeans[7]. Most commercially relevant algal species grow well on either seawater or wastewater, both of which are inexpensive alternatives to freshwater[8].

For economical reasons, the majority of algal aquacultures today are open-air[5]. While this normally vastly opens up the risk for contamination, several of the most important algae, such as Chlorella, Spirulina and Dunaliella have traits that allow them to outgrow competitors under certain optimal conditions. That said, the future of algal biofuel reactors is likely to take place under closed conditions. Maximized efficiency in biofuel production is highly dependent on non-contamination and the absence of certain heavy metal pollutants that would be present in open aquacultures.

Summary of various Aquaculture methods[5].
Summary of various Aquaculture methods[5].

Photosynthetic Efficiency

C. reinhardtii with mutated chlorophyll show far more photosynthetic activity (as measured by oxygen evolution) at high intensities than wild type[9].

The fundamental source of energy in biofuels is sunlight. Converting the electromagnetic energy in photons to chemical energy in reduced carbons is the core of biofuel production. Logically, one key focus of biofuel production would be to maximize the amount of energy absorbed during this first step.

Photoinhibition, a result of the oxidative damage done to electron carriers in light reactions, is one of the first hurdles to jump[10]. Truncated chlorophyll antennae show reduced levels of photoinhibition, resulting in less down time and therefore greater photosynthetic efficiency during the light reactions. In large growth cultures, antenna truncation also reduces light scattering, allowing greater access to appropriately energetic photons to the cells further from the surface of the liquid.

In wild type Chlamydomonas reinhardtii, the amount of energy in photons absorbed by chlorophyll exceeds the amount of energy that can be used to reduce the next protein in the photosynthetic pathway, Pheophytin[9]. DNA insertional mutagenesis was used to mutate nuclear genes for caretenoid and chlorophyll synthesis, such as lor1, npq2, cao' and TLA1. All genes except for npq2 showed promising results in antennae reduction leading to greater photosynthetic efficiency.

Lipid Content

Maximizing the lipid content of algae allows for the greatest energy content per unit land, and increases the energy return on extraction processes[4]. In chlorella, lipid accumulation tends to be greatest during the stationary phase. Media rich in iron, nitrate and phosphate is optimal in chlorella for maximum lipid content, with the highest achieved lipid dry cell volume being 55%.

One obvious problem to maximizing lipid yield is the fact that cells often use lipids as an energy source through β-oxidation. Inhibition of β-oxidation through gene knockouts is quite useful for algae grown under constant light conditions, but does retard growth in algae grown under natural light[11]. In the absence of light, algae typically live off of triacyl glyecerol; if they are deprived of this food source, their growth rate is inhibited, and net lipid production over time may actually be lower than in algae that are wild type for lipid catabolism.

The lipids that cells produce normally have to be chemically treated to form ethyl esters that can be directly used as biofuel[11]. Overexpression of ethanol synthesis from Zymomonas mobilis and wax ester synthase genes from Acinetobacter baylyi in E. coli produced slightly better ethyl ester yields, although these yields are expected to be greater in microalgae.

It is also possible to make long chain alkanes using algae. Microalga B. braunii is known to produce di- or tri-ene single chain hydrocarbons, but the chain lengths it produces (mostly >22 C) are too long to be directly used as fuel[11].

iGEM Connection

Reduction of fatty acid intermediates into alkanes.
Reduction of fatty acid intermediates into alkanes.

The University of Washington's 2011 iGem Team attempted to use Fatty Acid intermediates into alkanes[12]. They used Acy-ACP Reductase (AAR) to convert long Acyl-ACPs into aldehydes, and then used Aldehyde Decarbonylase (ADC) to convert them into alkanes.

AAR and ADC are both taken from Synecocchus elongatus[12]. The primary products of expression in E. coli were 13, 15 and 17 C alkanes, with a small component of 17 C alkenes. They claim to be trying (as of fall 2011) to increase yields, change the primary products and shift the system into yeast.

Both parts allegedly work.


  1. Biodiesel production—current state of the art and challenges [Vasudevan]
  2. What is Photosynthesis?


  3. Biodiesel from microalgae beats bioethanol


  4. Green microalga Chlorella vulgaris as a potential feedstock for biodiesel


  5. Commercial production of microalgae: ponds, tanks, tubes and fermenters


  6. DOE biofuel datasheet


  7. Microalgal Reactors: A Review of Enclosed System Designs and Performances


  8. Truncated chlorophyll antenna size of the photosystems—a practical method to improve microalgal productivity and hydrogen production in mass culture


  9. Solar energy conversion efficiencies in photosynthesis: Minimizing the chlorophyll antennae to maximize efficiency


  10. Genetic Engineering of Algae for Enhanced Biofuel Production


  11. UW Diesel Production


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