Synthetic Biology:DNA synthesis order reviews

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Revision as of 13:52, 12 June 2008 by Austin J. Che (Talk | contribs)
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Information that might be useful includes

  2. Order
    • Description
    • Approximate length
  3. DNA synthesis company
  4. Experience
    • Cost (both estimated and final)
    • Turnaround time (both estimated and final)
  5. Any other details that might be relevant

pSB4*5 BioBricks vector

  1. Reshma Shetty, MIT
  2. BioBricks vector components and vectors
    • 4 resistance markers (1kb each)
    • pSC101 origin (2kb)
  3. Codon Devices
  4. Experience
    • The resistance markers and pSC101 replication origin were synthesized as ordered. The vector scaffold had some mutations (some due to their issues during synthesis and some possibly due to my design flaws). Codon Devices opted to not pursue the project further at this point and did not deliver on the 3 vectors. [Additional note: It turns out that the vector scaffold they delivered was actually two vector copies fused together in such a way that when cut by any single enzyme, it looked the right size. Only running the uncut vector on the gel revealed the unusually large fragment size.]


IDT miniGenes

  1. Austin Che, MIT
  2. I've ordered a couple times from them, each less than 500nt ribozyme sequence
    • It is not being expressed so there should be no issues of cloning. It does have a lot of secondary structure in the RNA (and thus also in the DNA).
  3. I looked at Geneart, DNA2.0, and IDT (miniGenes) and decided to go with IDT
  4. Experience
    • Estimated cost = final cost = cost on their website ($95+$0.65/bp). Their cost is competitive with Geneart.
    • No surprises at all and didn't need to go through online forms to "request quotes"
    • From order, to shipment, it was 6 business days! I had the plasmid in hand 10 days after ordering.
  5. Overall, very happy. For < 500nt sequences, IDT minigenes is excellent.
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