{"entity": "publication", "iuid": "8f5e094a933447ccbc5cdaa743aeec5e", "timestamp": "2019-08-17T23:31:55.411Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8f5e094a933447ccbc5cdaa743aeec5e.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8f5e094a933447ccbc5cdaa743aeec5e"}}, "title": "MicroRNA expression profiles in non\u2011epithelial ovarian tumors.", "authors": [{"family": "Chang", "given": "Roger K", "initials": "RK"}, {"family": "Li", "given": "Xidan", "initials": "X"}, {"family": "Mu", "given": "Ninni", "initials": "N"}, {"family": "Hrydziuszko", "given": "Olga", "initials": "O"}, {"family": "Garcia-Majano", "given": "Beatriz", "initials": "B"}, {"family": "Larsson", "given": "Catharina", "initials": "C"}, {"family": "Lui", "given": "Weng-Onn", "initials": "WO"}], "type": "journal article", "published": "2018-01-00", "journal": {"volume": "52", "issn": "1791-2423", "issue": "1", "pages": "55-66", "title": "Int. J. Oncol."}, "abstract": "Ovarian germ cell tumors (OGCTs) and sex cord stromal tumors (SCSTs) are rare gynecologic tumors that are derived from germ and stromal cells, respectively. Unlike their epithelial counterparts, molecular pathogenesis of these tumor types is still poorly understood. Here, we characterized microRNA (miRNA) expression profiles of 9\u00a0OGCTs (2\u00a0malignant and 7\u00a0benign) and 3\u00a0SCSTs using small RNA sequencing. We observed significant miRNA expression variations among the three tumor groups. To further demonstrate the biological relevance of our findings, we selected 12\u00a0miRNAs for validation in an extended cohort of 16\u00a0OGCTs (9\u00a0benign and 7\u00a0malignant) and 7\u00a0SCSTs by reverse transcription-quantitative polymerase chain reaction. Higher expression of miR\u2011373\u20113p, miR\u2011372\u20113p and miR\u2011302c\u20113p and lower expression of miR\u2011199a\u20115p, miR\u2011214\u20115p and miR\u2011202\u20113p were reproducibly observed in malignant OGCTs as compared to benign OGCTs or SCSTs. Comparing with benign OGCTs, miR\u2011202c\u20113p and miR\u2011513c\u20115p were more abundant in SCSTs. Additionally, we examined Beclin\u00a01 (BECN1), a target of miR\u2011199a\u20115p, in the clinical samples using western blot analysis. Our results show that BECN1 expression was higher in malignant OGCTs than benign OGCTs, which is concordant with their lower miR\u2011199a\u20115p expression. This study suggests that these miRNAs may have potential value as tumor markers and implications for further understanding the molecular basis of these tumor types.", "doi": "10.3892/ijo.2017.4200", "pmid": "29138809", "labels": {"Bioinformatics Support and Infrastructure": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC5743337"}], "notes": null, "qc": null, "created": "2019-01-15T08:05:18.683Z", "modified": "2019-01-15T08:05:18.694Z"}