Systemic modulation of regulatory components by HDAC2 in G1/S cell cycle transition.

Hep3B cells were transfected with no siRNA (None), oligofectamine only (Reag), 100 nmol/L scrambled siRNA (Scr) or 100 nmol/L of two different HDAC2 specific siRNAs (#1 and #2), and were harvested at 72 h post-transfection. (A) Immunoblotting of CDKs, cyclins and CDK inhibitors of G1/S transition were performed. (B) Effects of HDAC2 depletion on pRb and E2F/DP1 target gene expressions. (C) Protein expressions of p21WAF1/Cip1, CDK2 and PCNA in human HCC tissues were analyzed by immunoblotting. All experiments were repeated three times with same results.