Illumina Sequence coverage of <i>Arabidopsis</i> cDNAs. Loren A. Honaas Eric K. Wafula Norman J. Wickett Joshua P. Der Yeting Zhang Patrick P. Edger Naomi S. Altman J. Chris Pires James H. Leebens-Mack Claude W. dePamphilis 10.1371/journal.pone.0146062.g001 <p>Coverage of all detected genes by sequencing reads. The darkest bar represents the number of detected genes not tagged, and each progressively lighter bar represents genes in a bin with a 5% increase in coverage, with the two lightest bars showing the number of genes covered at >90% and >99%, respectively. Normalized BR12 = Normalized Illumina library from pooled biological replicates 1 and 2, BR12 = combined coverage of Illumina biological replicates 1 and 2, BR1 = Illumina biological replicate 1, BR2 = Illumina biological replicate 2.</p> 2016-01-18 14:26:08 assembler benchmark Illumina transcriptome data SCERNA Oryza sativa transcriptomes Arabidopsis leaf transcriptome 99 transcriptome assemblies Lessons Learned transcriptome assembly strategies Arabidopsis thaliana chimeric assemblies abundance transcripts clc Normalized libraries assembly improvement Superior De Novo Transcriptome Assemblies Controlled analyses transcriptome assemblies lack 1000 Plant Genome Arabidopsis genes