RT Journal Article SR Electronic T1 Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis. JF Molecular and Cellular Biology JO Mol. Cell. Biol. FD American Society for Microbiology SP 2051 OP 2062 DO 10.1128/MCB.15.4.2051 VO 15 IS 4 A1 Houge, G A1 Robaye, B A1 Eikhom, T S A1 Golstein, J A1 Mellgren, G A1 Gjertsen, B T A1 Lanotte, M A1 Døskeland, S O YR 1995 UL http://mcb.asm.org/content/15/4/2051.abstract AB Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied by internucleosomal DNA fragmentation, with cleavage of 28S rRNA and of DNA being linked temporally. This indicates that 28S rRNA fragmentation may be as general a feature of apoptosis as internucleosomal DNA fragmentation and that concerted specific cleavage of intra- and extranuclear polynucleotides occurs in apoptosis. Apoptosis-associated cleavage sites were mapped to the 28S rRNA divergent domains D2, D6 (endothelial cells), and D8. The D2 cuts occurred in hairpin loop junctions considered to be buried in the intact ribosome, suggesting that this rRNA region becomes a target for RNase attack in apoptotic cells. D8 was cleaved in two exposed UU(U) sequences in bulge loops. Treatment with agents causing necrotic cell death or aging of cell lysates failed to produce any detectable limited D2 cleavage but did produce a more generalized cleavage in the D8 region. Of potential functional interest was the finding that the primary cuts in D2 exactly flanked a 0.3-kb hypervariable subdomain (D2c), allowing excision of the latter. The implication of hypervariable rRNA domains in apoptosis represents the first association of any functional process with these enigmatic parts of the ribosomes.