Reveal 2.0 for E. coli O157:H7 Medium, 500 g

Item No.  9728

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Out of stock
Expect availability in 2-4 weeks
Analyte E. coli O157:H7
Approvals AOAC-RI 011103
Brand Reveal®
Limit of Detection 1 cfu/analytical unit
Mass 500 g
Package Dimensions 3.60 IN x 3.60 IN x 6.50 IN
Package Weight 0.50 LB
Platform Lateral Flow
Quantity per Package 1 unit
Result Type Presence/Absence
Testing Time 10.00 Minutes
Time to Result 8 - 20 Hours

  1. 20 Reveal 2.0 for E. coli O157:H7 test devices
  2. 20 transfer pipettes
  3. 20 sample cups
  4. 1 promoter reagent
  1. Stomacher-type bags (Neogen® item 9736)
  2. Scale capable of weighing a minimum of 65 g (Neogen item 9427)
  3. Timer (Neogen item 9416)
  4. Sample cup rack (Neogen item 9475)
  5. Sterile deionized or distilled water
  6. Incubator capable of maintaining 42 ± 1°C (Neogen item 9735)

Test Systems:

NOTE: The Reveal 2.0 for E. coli O157:H7 device requires the use of certain enrichment media and supplies. These can be purchased as part of a test system or individually.

  1. Reveal 2.0 for E. coli O157:H7 complete system (contains devices and media for 20, 65 g samples) (Neogen item 9714)


NOTE: Media can be purchased individually or as part of a test system.

  1. Reveal 2.0 for E. coli O157:H7 media
    1. Unitized (Neogen item 9727)
    2. Bulk 500 g (Neogen item 9728)
    3. 2 kg (Neogen item 9728B)

Optional Materials

  1. Stomacher 400 machine or equivalent

The system utilizes specified enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal media, the system can provide results in 12 to 20 hours.

A portion (200 µL) of the enriched culture is placed into the sample cup followed by a chemical treatment. The test device is placed into the sample cup during the chemical treatment and is incubated at 42°C for 15 minutes. The sample is wicked through the reagent zone that contains specific anti-E. coli O157:H7 antibodies conjugated to colloidal gold particles.

If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigenantibody complex then leaves the reagent zone and travels across the membrane, which contains a zone of anti-E. coli O157:H7 antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.

The reagent zone also contains a gold conjugate of a second antigen, which is eluted by the sample regardless of the presence of E. coli O157:H7 antigen to form a line in the control zone, ensuring the test is working properly.




