@article {Jing483347, author = {Jing, Wenwen and Wang, Yan and Yang, Yunze and Wang, Yi and Ma, Guangzhong and Wang, Shaopeng and Tao, Nongjian}, title = {Time-resolved digital immunoassay for rapid and sensitive quantitation of procalcitonin with plasmonic imaging}, elocation-id = {483347}, year = {2018}, doi = {10.1101/483347}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of ~ 3 pg/mL, dynamic range of 4-12500 pg/mL, for a total detection time of ~ 25 mins.}, URL = {https://www.biorxiv.org/content/early/2018/11/29/483347}, eprint = {https://www.biorxiv.org/content/early/2018/11/29/483347.full.pdf}, journal = {bioRxiv} }