The Eukaryotic Linear Motif resource for
Functional Sites in Proteins
Functional site class:
HEAT Domain Ligand
Functional site description:
Docking motif for PIKK kinases family found in DNA damage proteins Nbs1, ATRIP and XRCC5.
ELM Description:
A study from Falck,2005 has shown that mutations of the amino acids EE, DD and RY are sufficient to inhibit the interaction of human Nbs1 with ATM. The same study has shown that mutation of residues EE and DD of the motif in Ku80 inhibits interaction with DNA-PKcs. Another study from You,2005 has shown that mutation of the residues DD in fission yeast Nbs1 abolishes the interaction with Tel1.
Pattern: [DEN][DEN].{2,3}[ILMVA][DEN][DEN]L
Pattern Probability: 0.0000313
Present in taxon: Eukaryota
Interaction Domain:
HEAT (PF02985) HEAT repeat (Stochiometry: 1 : 1)
o See 4 Instances for DOC_PIKK_1
o Abstract
DNA damage response pathways involve three major groups of evolutionarily conserved proteins that act together to translate the signal of damaged DNA into responses of cell cycle arrest and DNA repair. These groups comprise sensor proteins that recognize damaged DNA directly or indirectly, transducer proteins - typically PIKKs such as ATM, ATR and DNA-PKcs - that relay and amplify the damage signal, and effector proteins that control cell cycle progression, chromatin restructuring and DNA repair. Recruitment of DNA damage-associated PIKKs to DNA lesions is thought to be a principal step in their activation and in their function in checkpoint signalling and DNA repair. Although these PIKKs have an affinity for DNA, recruitment to DNA lesions is facilitated by specific partner proteins. For example, recruitment of ATR to single-stranded DNA (ssDNA) requires ATRIP and DNA-PKcs recruitment to DNA double-strand breaks (DSBs) is mediated by the Ku70-Ku80 heterodimer.
o 3 selected references:

o 3 GO-Terms:

o 4 Instances for DOC_PIKK_1
Acc., Gene-, NameStartEndSubsequenceLogic#Ev.OrganismNotes
Q6XV80 nbn
744 751 QTQQVREESLAEDLFRYNPK TP 2 Xenopus laevis (African clawed frog)
P13010 XRCC5
720 728 GDTAAVFEEGGDVDDLLDMI TP 2 Homo sapiens (Human)
769 776 PDVTDCEEAALDDLCAAETD TP 1 Homo sapiens (Human)
O60934 NBN
736 743 QNQHAKEESLADDLFRYNPY TP 6 Homo sapiens (Human)
