PT - JOURNAL ARTICLE AU - Sakamoto, Kei AU - Kim, Yun-Gi AU - Hara, Hideki AU - Kamada, Nobuhiko AU - Caballero-Flores, Gustavo AU - Tolosano, Emanuela AU - Soares, Miguel P. AU - Puente, José L. AU - Inohara, Naohiro AU - Núñez, Gabriel TI - IL-22 controls iron-dependent nutritional immunity against systemic bacterial infections AID - 10.1126/sciimmunol.aai8371 DP - 2017 Feb 03 TA - Science Immunology PG - eaai8371 VI - 2 IP - 8 4099 - 4100 - SO - Sci. Immunol.2017 Feb 03; 2 AB - Actively killing pathogens is an important function of the immune response; equally important are mechanisms that limit nutrient availability to the pathogen, termed nutritional immunity. The cytokine interleukin-22 (IL-22) plays an essential role in resolution of infections at epithelial barrier sites, including skin, lungs, and intestines. Using a systemic model of Citrobacter rodentium infection, Sakamoto et al. have uncovered an unexpected role for IL-22 in limiting availability of iron to the pathogen by promoting increased production of heme scavengers from the liver. Their studies extend the role of IL-22 beyond barrier sites and establish a previously unappreciated role for IL-22 in regulating nutritional immunity in the context of systemic bacterial infections.Host immunity limits iron availability to pathogenic bacteria, but whether immunity limits pathogenic bacteria from accessing host heme, the major source of iron in the body, remains unclear. Using Citrobacter rodentium (a mouse enteric pathogen) and Escherichia coli (a major cause of sepsis in humans) as models, we find that interleukin-22 (IL-22), a cytokine best known for its ability to promote epithelial barrier function, also suppresses the systemic growth of bacteria by limiting iron availability to the pathogen. To understand the mechanistic basis of IL-22–dependent iron retention in the host, using an unbiased proteomic approach, we have identified that IL-22 induces the production of the plasma hemoglobin scavenger haptoglobin and the heme scavenger hemopexin. Moreover, the antimicrobial effect of IL-22 depends on the induction of hemopexin expression, whereas haptoglobin was dispensable. Impaired pathogen clearance in infected Il22−/− mice was restored by hemopexin administration, and hemopexin-deficient mice had increased pathogen loads after infection. These studies reveal a previously unrecognized host defense mechanism regulated by IL-22 that relies on the induction of hemopexin to limit heme availability to bacteria, leading to suppression of bacterial growth during systemic infections.