TY - JOUR DP - National Agricultural Library DB - PubAg JO - Plant science TI - SoHSC70 positively regulates thermotolerance by alleviating cell membrane damage, reducing ROS accumulation, and improving activities of antioxidant enzymes A1 - Qi, Chuandong A4 - Qi, Chuandong A4 - Lin, Xinpeng A4 - Li, Shuangtao A4 - Liu, Lun A4 - Wang, Zhirong A4 - Li, Yu A4 - Bai, Ruyue A4 - Xie, Qian A4 - Zhang, Na A4 - Ren, Shuxin A4 - Zhao, Bing A4 - Li, Xiangdong A4 - Fan, Shuangxi A4 - Guo, Yang-Dong EP - 2019 v.283 KW - Arabidopsis KW - Spinacia oleracea KW - ascorbate peroxidase KW - callus KW - catalase KW - cell membranes KW - electrical conductivity KW - environmental factors KW - enzyme activity KW - gene silencing KW - heat shock proteins KW - heat stress KW - heat tolerance KW - heat treatment KW - malondialdehyde KW - peroxidase KW - photosynthesis KW - plant growth KW - reactive oxygen species KW - seedlings KW - spinach KW - superoxide dismutase KW - temperature AN - 6336338 AB - High temperature is a major environmental factor affecting plant growth. Heat shock proteins (Hsps) are molecular chaperones that play important roles in improving plant thermotolerance during heat stress. Spinach (Spinacia oleracea) is very sensitive to high temperature; however, the specific function of Hsps in spinach is unclear. In this study, cytosolic heat shock 70 protein (SoHSC70), which was induced by heat stress, was cloned from spinach. Overexpressing SoHSC70 in spinach calli and Arabidopsis enhanced their thermotolerance. In contrast, spinach seedlings with silenced SoHSC70 by virus-induced gene silencing (VIGS) showed more sensitivity to heat stress. Further analysis revealed that overexpressing SoHSC70 altered relative electrical conductivity (REC), malondialdehyde (MDA) content, photosynthetic rate, reactive oxygen species (ROS) accumulation and the activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) after the heat treatment. Taken together, our results suggest that overexpressing SoHSC70 positively affects heat tolerance by reducing membrane damage and ROS accumulation and improving activities of antioxidant enzymes. PY - 2019 LA - DA - 2019-06 VL - v. 283 SP - pp. 385-395 DO - 10.1016/j.plantsci.2019.03.003 ER -