TY - JOUR T1 - Growth-dependent activation of protein kinases links cell cycle progression to cell growth JF - bioRxiv DO - 10.1101/610469 SP - 610469 AU - Jasani, Akshi AU - Huynh, Tiffany AU - Kellogg, Douglas R. Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/04/16/610469.abstract N2 - In all cells, progression through the cell cycle occurs only when sufficient growth has occurred. This fundamental linkage shows that cells can measure growth, but the underlying mechanisms are poorly understood. Here, we investigate how budding yeast cells measure growth. Cells that lack the related kinases Gin4 and Hsl1 show excessive bud growth during a prolonged cell cycle delay, which suggests that they fail to detect bud growth. Moreover, Gin4 and Hsl1 undergo hyperphosphorylation that is proportional to bud growth and dependent upon binding to anionic phospholipids that are delivered to the growing bud. Gin4 and Hsl1 have previously been shown to relay signals that control both cell growth and cell cycle progression. Together, the data suggest that addition of phospholipids to the plasma membrane generates a gradually increasing signal that triggers cell cycle progression when sufficient growth has occurred. The data further suggest that control of cell size and cell growth are mechanistically linked. ER -