TY - JOUR T1 - Mechanisms of naturally evolved ethanol resistance in <em>Drosophila melanogaster</em> JF - The Journal of Experimental Biology JO - J. Exp. Biol. SP - 3996 LP - 4003 DO - 10.1242/jeb.110510 VL - 217 IS - 22 AU - Fry, James D. Y1 - 2014/11/15 UR - http://jeb.biologists.org/content/217/22/3996.abstract N2 - The decaying fruit in which Drosophila melanogaster feed and breed can contain ethanol in concentrations as high as 6–7%. In this cosmopolitan species, populations from temperate regions are consistently more resistant to ethanol poisoning than populations from the tropics, but little is known about the physiological basis of this difference. I show that when exposed to low levels of ethanol vapor, flies from a tropical African population accumulated 2–3 times more internal ethanol than flies from a European population, giving evidence that faster ethanol catabolism by European flies contributes to the resistance difference. Using lines differing only in the origin of their third chromosome, however, I show that faster ethanol elimination cannot fully explain the resistance difference, because relative to African third chromosomes, European third chromosomes confer substantially higher ethanol resistance, while having little effect on internal ethanol concentrations. European third chromosomes also confer higher resistance to acetic acid, a metabolic product of ethanol, than African third chromosomes, suggesting that the higher ethanol resistance conferred by the former might be due to increased resistance to deleterious effects of ethanol-derived acetic acid. In support of this hypothesis, when ethanol catabolism was blocked with an Alcohol dehydrogenase mutant, there was no difference in ethanol resistance between flies with European and African third chromosomes. ER -