restriction fragment length polymorphism


re·stric·tion frag·ment length pol·y·mor·phism (RFLP),

used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins, the distance (in nucleotides on the chromosome) can be different between two genetic markers, usually because of repeated base patterns.

re·stric·tion frag·ment length pol·y·mor·phism

(RFLP) (rĕ-strik'shŭn frag'mĕnt length pol'ē-mōrf'izm)
Used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins there is often wide (but measurable) sequence variety between people.

restriction fragment length polymorphism (RFLP)

Variations within a species in the lengths of fragments of DNA caused by RESTRICTION ENZYMES. The variations are caused by mutations that either abolish the normal sites of breakage or create new ones, characteristic of the mutations. RFLP analysis may allow genetic abnormalities to be detected, often before birth, even if the location of the mutated gene or genes is unknown.

restriction fragment length polymorphism (RFLP)

a MUTATION that results in a change in the pattern of restriction fragments generated when a DNA molecule is treated with a RESTRICTION ENZYME (see RESTRICTION ANALYSIS). The RFLP is apparent when the fragments are resolved by AGAROSE GEL ELECTROPHORESIS. RFLPs can be used as reference LOCI for mapping, in relation to specific linked genes (see GENETIC LINKAGE). RFLPs can be used as a diagnostic test for genetic DISEASES, by detecting specific gene defects. The presence of the RFLP may be a direct indication of the gene defect, or may be closely linked to it. Diseases that have heen diagnosed by RFLP analysis include certain types of THALASSAEMIA and HAEMOPHILIA, and by RFLP linkage analysis, HUNTINGTON'S CHOREA.
References in periodicals archive ?
Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communities.
Carrier detection in haemophilia B using two futher intragenic restriction fragment length polymorphisms. Nucleic Acid Res.
Restriction fragment length polymorphism (RFLP-PCR) was used to identifyVDR genotypes.
Molecular identification was conducted at Seoul National University Bundang Hospital, where PCR restriction fragment length polymorphism and multiplex real-time PCR and melting curve analyses were performed as described (5,6).
The BsmI vitamin D receptor restriction fragment length polymorphism (bb) influences the effect of calcium intake on bone mineral density.
Probes have a higher specificity toward a given polymorphism than does restriction fragment length polymorphism genotyping (6).
tuberculosis isolates from all seven patients were genotyped by using spoligotyping, mycobacterial interspersed repetitive units analysis, and IS6110-based restriction fragment length polymorphism analysis.
Abbreviations: bp, base pair; RAFLP, restriction amplification fragment length polymorphic; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism.
The scientists verified their findings by making DNA fingerprints using restriction fragment length polymorphism techniques.
Such cuts, usually much scarcer in a single gene, are the basis of the genetic technique called restriction fragment length polymorphism (RFLP) analysis (SN: 8/31/85, p.
Typing and molecular epidemiology of some black fungi based on analysis of the restriction fragment length polymorphism in the mitochondrial DNA.
The assays used to identify these two species include both genotypic methods like polymerase chain reaction (PCR) (mig), PCR with restriction fragment length polymorphism (PCR-RFLP) (hsp65), probe hybridization and sequencing (16S rRNA) and phenotypic methods like high performance liquid chromatography (HPLC) and serotyping (2).

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