

If I requested a homozygous knock-in and only a heterozygous mutation was produced, would Horizon be able to determine the extent of mutant vs wt expression?

Assessment of mutant vs WT expression at the RNA level is something we carry out as standard as part of every targeting project (where applicable). We use both standard RT-PCR followed by Sanger sequencing, and quantative droplet digital PCR (ddPCR) to assess level of expression. However, we do not guarantee to be able to deliver clones which express the mutation at a particular level.