DSpace Collection: http://hdl.handle.net/10174/14490 2020-08-06T20:18:19Z Atividades Inclusivas no Laboratório HERCULES http://hdl.handle.net/10174/26807 Title: Atividades Inclusivas no Laboratório HERCULES Authors: Ferreira, Teresa; Nunes, Margarida Editors: Candeias, Adelinda Abstract: Em maio de 2014 o Laboratório HERCULES numa organização coordenada com a ASCTE – Associação Sócio-Cultural Terapêutica de Évora realizou um workshop sobre pigmentos naturais e artificiais, ministrado por alunos de primeiro e segundo ciclo da UÉ a alunos desta associação. O objetivo foi promover uma prática pedagógica que visava a inclusão social através do desenvolvimento de atividades de cariz artístico e científico, num ambiente lúdico, fomentando a troca de ideias e práticas entre pessoas com necessidades educativas especiais e alunos em diferentes ciclos de estudos da UÉ.Pretendeu-se ainda que esta atividade contribuísse para a sua inclusão deste grupo no espaço universitário e lhes proporcionasse igualdade de oportunidades educativas e sociais. 2019-12-01T00:00:00Z Coumarins as Fluorescent Labels of Biomolecules. In Applications of Coumarin Derivatives http://hdl.handle.net/10174/26674 Title: Coumarins as Fluorescent Labels of Biomolecules. In Applications of Coumarin Derivatives Authors: Pereira, A; Martins, S; Caldeira, A T Editors: Rao, Venketeshwer Abstract: Important areas such as environmental sciences, medicine, pharmacy, and cellular biology are dependent on very sensitive analytical techniques. One of the most common methodologies used for their bioanalytical purposes is the fluorescent labelling. The synthesis of new fluorophores and the great development of fluorescent-labelling techniques combined with the enormous technological advances in the field of fluorescence microscopy allowed to deepen the structural knowledge of biomolecules. This new organic fluorophores form covalent bonds with the sample to be analyzed, producing stable bioconjugates that show fluorescence in a wide range of wavelengths, depending on the label used. Coumarin derivatives represent one of the most important chemical classes of organic fluorescent materials being one of the most extensively investigated and commercially significant groups of organic fluorescent materials. In this chapter, it is reviewed the use of fluorescent coumarin derivatives and their application to labelling biomolecules. These fluorescent labels allow researchers to study, and understand, biomolecular assemblies that exhibit complex sensitivity and selectivity. Reactive fluorescent coumarin derivatives are actually widely used in labelling biomolecules as peptides, proteins, oligonucleotides, nucleic acids, and carbohydrates, among other biological molecules. 2019-01-01T00:00:00Z Stone biodegradation and mitigation—the case of Convent of Christ, Portugal http://hdl.handle.net/10174/25548 Title: Stone biodegradation and mitigation—the case of Convent of Christ, Portugal Authors: Rosado, T; Silva, M; Santos, R; Galvao, A; Mirão, J; Candeias, A; Caldeira, A Teresa Editors: Mosquera, Almoraima Gil, M M. J Abstract: Convent of Christ (UNESCO’s World Heritage Monument, Tomar-Portugal) is one of the most expressive testimonies of Portuguese architecture, whose huge structure include seven cloisters, different monastic constructions and the emblematic Chapter Window (or Manueline Window). Unfortunately, over the years the stone materials have been subjected to several damages, which have modified the appearance of the surfaces and their integrity. Attending to the negative effects of the microbial population on the rock surfaces, our research is actively pursuing the microorganisms responsible for biodeterioration/biodegradation and attempting signalise the biodeteriogenic agents. Furthermore, the development of mitigation strategies based on alternative green solutions, have been successfully tested in simulation assays, for promoting the safeguard of these stone materials. Natural biocompounds produced in our laboratory (Bevotech) reveal effective inhibition capacity (in vitro and in situ assays) and do not induce alterations on the rock materials. These compounds seem to be a green/ecofriendly solution for Cultural Heritage safeguard plan. 2018-01-01T00:00:00Z Biodeterioration of easel paintings – An overview http://hdl.handle.net/10174/25301 Title: Biodeterioration of easel paintings – An overview Authors: Caldeira, A Teresa; Salvador, C; Rosado, T; Candeias, A Abstract: In summary, the microbial community plays an essential role in the degradation of easel paintings. Modern chemical and genetic techniques permit both early detection of biodeterioration and detailed investigation of the degradation processes. Remediation and preservation technologies are still in the early stages of development. 2018-01-01T00:00:00Z