@article {Chalker143, author = {Chalker, A F and Okely, E A and Davison, A and Leach, D R}, title = {The effects of central asymmetry on the propagation of palindromic DNA in bacteriophage lambda are consistent with cruciform extrusion in vivo.}, volume = {133}, number = {2}, pages = {143--148}, year = {1993}, publisher = {Genetics}, abstract = {The propagation of lambda phages carrying long perfect palindromes has been compared with that of phages carrying imperfect palindromes with small regions of central asymmetry. The perfect palindromes confer a more deleterious phenotype than those with central asymmetry and the severity of the phenotype declines with the length of asymmetry in the range from O to 27 base pairs. These results argue that a center-dependent reaction is involved in the phenotypic effects of palindromic DNA sequences, consistent with the idea that cruciform extrusion occurs in vivo.}, issn = {0016-6731}, URL = {https://www.genetics.org/content/133/2/143}, eprint = {https://www.genetics.org/content/133/2/143.full.pdf}, journal = {Genetics} }