@article {Caron3105, author = {Caron, Etienne and Kowalewski, Daniel J. and Chiek Koh, Ching and Sturm, Theo and Schuster, Heiko and Aebersold, Ruedi}, title = {Analysis of Major Histocompatibility Complex (MHC) Immunopeptidomes Using Mass Spectrometry}, volume = {14}, number = {12}, pages = {3105--3117}, year = {2015}, doi = {10.1074/mcp.O115.052431}, publisher = {American Society for Biochemistry and Molecular Biology}, abstract = {The myriad of peptides presented at the cell surface by class I and class II major histocompatibility complex (MHC) molecules are referred to as the immunopeptidome and are of great importance for basic and translational science. For basic science, the immunopeptidome is a critical component for understanding the immune system; for translational science, exact knowledge of the immunopeptidome can directly fuel and guide the development of next-generation vaccines and immunotherapies against autoimmunity, infectious diseases, and cancers. In this mini-review, we summarize established isolation techniques as well as emerging mass spectrometry-based platforms (i.e. SWATH-MS) to identify and quantify MHC-associated peptides. We also highlight selected biological applications and discuss important current technical limitations that need to be solved to accelerate the development of this field.}, issn = {1535-9476}, URL = {https://www.mcponline.org/content/14/12/3105}, eprint = {https://www.mcponline.org/content/14/12/3105.full.pdf}, journal = {Molecular \& Cellular Proteomics} }