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Articles by Agnes Murdiati
Total Records ( 11 ) for Agnes Murdiati
  Fatma Zuhrotun Nisa , Mary Astuti , Agnes Murdiati and Sofia Mubarika Haryana
  Background and Objective: Breast cancer is the most frequently diagnosed cancer in women. Chemotherapy is the main method of breast cancer treatment but there are side effects. Carica papaya leaves is vegetable foods consumed by most people of Indonesia have potential as anticancer. The aim of this study was to investigate anti-proliferative and apoptotic induced effect of aqueous papaya leaves extracts on human breast cancer cell lines MCF-7. Materials and Methods: Inhibitory on cell proliferation was measured by MTT assay while apoptosis induction was measured using Annexin V. Results: The results showed that papaya leaf can inhibit the proliferation of human breast cancer cells MCF-7 with IC50 in 1319.25 μg mL–1. The IC50 values of papaya leaf extract was higher than the IC50 value quercetin and doxorubicin. Papaya leaf extract can also induce apoptosis of breast cancer cells MCF-7 about 22.54% for concentration 659.63 μg mL–1 and about 20.73% for concentration 329.81 μg mL–1. The percentage of cell apoptosis of papaya leaf extract lower than doxorubicin but higher than quercetin. Conclusion: This study indicated that papaya leaf extract have potential as anticancer through mechanism anti-proliferation and apoptosis induction.
  Nurhidajah , Mary Astuti , Sardjono and Agnes Murdiati
  The objective of this study was to evaluate the glycaemic property of diabetic rats after consumption of enriched pigmented rice. During the experiment, thirty 2.5-month-old male Wistar rats with 175-250 g body weights were divided into 6 groups (n = 6) and fed Mandel Handayani pigmented rice enriched with 2% kappa-carrageenan and anthocyanin extract from black rice (5 ml/100 g pigmented rice) and standard feed AIN 93 for maintenance. After 5 days of adaptation, diabetes was induce using STZ and nicotinamide and treatment was started 5 days afterwards for 6 weeks. The observed parameters were feed remains and efficiency, body weight, blood glucose level, serum insulin assayed using a rat ELISA insulin kit and Homa IR and Homa β analyses. The results showed that both pigmented rice and kappa-carrageenan and anthocyanin-enriched pigmented rice improved the glycaemic properties of diabetic rats, as indicated by increases in rat body weight of between 10.14 and 11.94%, with feed efficiency ranging between 13.62 and 17.39%, reduced and stabilized blood glucose levels, increased insulin levels, reduced insulin resistance (HOMA IR) and improved pancreatic β cell function (HOMA β).
  Priyanto Triwitono , Yustinus Marsono , Agnes Murdiati and Djagal Wiseso Marseno
  Objective: This study was conducted to evaluate the physiological effects of mung bean starch RS-3 on the obesity index and adipose cell profile of Sprague-Dawley rats. Methodology: A total of 35 male, 1 month old Sprague-Dawley rats were divided into 5 groups and given Fructose Medium Fat (FMF) feed containing Corn Starch (CS), mung bean starch (MS) or Resistant Starch (RS) for 6 weeks. In addition, the diet AIN 93G was used as the standard (STD) feed. Results: The results showed that the rats that were fed the RS diet (FMF-RS3-14 and FMF-RS3-28) had a lower feed intake and weight gain compared to those fed the fructose medium fat-corn starch (FMF-CS) or FMF-mung bean starch (FMF-MS) diets. The intervention of the FMF-CS diet led to an increase in the obesity index, whereas, the RS diet decreased the obesity index. Conclusion: The replacement of CS with RS at a dose of 28 g/1000 cal (FMF-RS3-28) for 6 weeks was effective in preventing obesity, with a Lee obesity index of 301.8.
  Veriani Aprilia , Agnes Murdiati , Pudji Hastuti and Eni Harmayani
  Objective: This study was conducted to describe the synthesis of carboxymethyl porang glucomannan (CPGM) and its physicochemical characteristics. Materials and Methods: The CPGM was synthesized by treating porang glucomannan (PGM) with sodium chloroacetate under basic conditions at different temperatures (50, 60 and 70°C) for different durations (20, 40 and 60 min). The CPGM products were then analyzed to determine what functional groups were present, the degree of substitution (DS), the water solubility, the zeta potential and the viscosity. Results: Carboxymethylation of the porang glucomannan was confirmed by the increase in the intensity of the carbonyl absorption peak in the fourier transform infrared (FTIR) spectra. Compared to the native porang glucomannan, the carboxymethylated porang glucomannan was more negatively charged and more water soluble but was less viscous. Both temperature and reaction time influenced the viscosity and water solubility, but the DS value was more influenced by reaction time than by temperature. Conclusion: Carboxymethylation of porang glucomannan yielded a more useful product than did native porang glucomannan. The increase in water solubility and decrease in viscosity made the product widely applicable in the food industry. The negatively charged polymer has the potential to interact with positively charged polymers to develop new products. Further research may be conducted to tune the reaction time to achieve certain properties that will suit additional needs.
  Siti Tamaroh , Sri Raharjo , Agnes Murdiati and Sri Anggrahini
  Background and Objective: Purple yam (Dioscorea alata L.) is a potential source of natural antioxidants due to its relatively high anthocyanin content. Anthocyanin is a natural source of antioxidants, acting as a free radical scavenger with a role in aging, cancer and degenerative illness prevention. Using a variety of solvents and acids, this study aimed to extract purple yam flour anthocyanins with high antioxidant activities. Methodology: Anthocyanins were extracted using methanol and ethanol-based solvents that were acidified with hydrochloric acid (HCl), citric acid or tartaric acid. The resulting extracts were assayed for anthocyanin and total phenolic contents, antioxidant activity (expressed as % radical-scavenger activity [%RSA]) and ferric reducing antioxidant power (FRAP). All data were analyzed using one-way analysis of variance. The differences were analyzed using Duncan’s multiple range test. The p<0.05 was considered significant. Pearson’s correlation coefficient analysis among anthocyanins, total phenolics contents and antioxidant activities were calculated using Microsoft Excel, 2007. Results: Results showed that the methanol/HCl (MeH) solvent could be used to extract anthocyanin from purple yam flour more thoroughly than other solvents. Total phenolic contents were not significantly different between MeH and methanol/tartaric acid (MeT) extracts (5.18 mg GA/100 g extract). Antioxidant activities of MeH and MeT anthocyanin extracts were not significantly different (69.87% RSA and FRAP of 50.27 μmol ε ferro L–1). Anthocyanin and total phenol contents correlated significantly with RSA and FRAP. Conclusion: This study suggested that anthocyanins and phenols purple yam flour are an abundant natural antioxidant sources, while the best solvent for the extraction was an acidified polar solvent.
  Atina Rahmawati , Agnes Murdiati , Yustinus Marsono and Sri Anggrahini
  Background and Objective: Complex carbohydrate is a mixture of dietary fibre and starch present in food. The maximum complex carbohydrate content of white jack bean (Canavalia ensiformis) can be achieved by treating white jack bean with three autoclaving-cooling cycles. The objectives of this study were to evaluate the effects of complex carbohydrate from white jack bean following autoclaving-cooling on hypercholesterolemic rats and to assess its bile acid binding ability (in vitro). Methodology: Thirty Sprague-Dawley rats were divided into the following 6 groups: K1, a healthy control group; K2, a negative control group (hypercholesterol); K3, a positive control group (simvastatin); K4, a group administered a diet containing complex carbohydrate flour (5%), K5: a group administered a diet containing complex carbohydrate flour treated with autoclaving-cooling (5%) and K6, a group administered a diet containing complex carbohydrate flour treated with autoclaving-cooling (10%). The intervention lasted 4 weeks. The parameters observed were body weight, lipid profile, short-chain fatty acid (SCFA) profile, digesta cholesterol and bile acid binding ability (in vitro). Results: The hypercholesterolemic rats of the K6 group, which were fed a diet containing complex carbohydrate flour treated with autoclaving-cooling, exhibited maintained body weight and an improved lipid profile equivalent to those of the K3 positive control group (simvastatin). The rats of the K6 group could produce SCFAs with an acetic:propionic:butyric molar ratio of 50:39:11. The complex carbohydrate flour treated with autoclaving-cooling was able to bind 17.54% of the cholic acid and 32.43% of the deoxycholic acid. The K6 group was able to bind 100.36 mg/100 g digesta cholesterol. Conclusion: The K6 group achieved the best results in terms of maintaining the body weight and improving the lipid profile of hypercholesterolemic rats to levels equivalent to those of the K3 positive control group (simvastatin). The K6 group also exhibited an improved SCFA molar ratio with the ability to bind bile acids (in vitro) and digesta cholesterol.
  Chrysanta Aurelia , Agnes Murdiati , Supriyanto and Andriati Ningrum
  Background and Objective: Jack beans are one of the most underutilized legumes in Indonesia and they are rich in protein. In Indonesia, several traditional products have been developed using jack beans as the raw material but the skin is not used, even though it contains dietary fiber, e.g., cellulose, that can be beneficial to human health. The valorization concept is a trending topic that uses byproducts, such as jack bean skin, to increase the value of the raw material. The objective of this research was to investigate the effects of the concentrations of sodium hydroxide (NaOH) and sodium hypochlorite (NaOCl) on several physicochemical characterizations of cellulose from jack bean skin. Materials and Methods: Cellulose isolation was carried out by delignification using NaOH at several concentrations (4, 6, 8, 10 and 20% NaOH), oxidation bleaching using NaOCl at different concentrations (0, 3 and 6% NaOCl) and reduction bleaching using 3% sodium bisulfite. The isolated cellulose from jack bean skins was analyzed and its physicochemical characteristics, e.g., yield, cellulose content, color (lightness), water holding capacity and oil holding capacity, were examined. Then, jack bean skin cellulose was analyzed to determine its functional groups using Fourier transform infrared (FT-IR) spectroscopy. Results: The highest yield of cellulose (46.40%) was obtained by using 4% NaOH. The highest cellulose content [76.79% (%db)] was obtained by using 10% NaOH. Increasing the NaOCl concentration helped lighten the cellulose color. Conclusion: The optimal sample treatment consisted of a combination of 10% NaOH and 3% NaOCl to produce a high cellulose content, light cellulose color and FT-IR peaks similar to commercial cellulose.
  Bagus Fajar Pamungkas , Supriyadi , Agnes Murdiati and Retno Indrati
  Background and Objective: The optimal use of haruan scales for preparing collagen is a promising method to increase value-added products and to protect the environment. The aim of this research was to characterize the acid- and pepsin-soluble collagens from haruan (Channa striatus) scales. Materials and Methods: The fish scales were subjected to an extraction with 0.5 M acetic acid and a digestion with 0.1% pepsin. The results are presented as the mean±standard deviation. Results: The yields of the acid-soluble collagen in the haruan scales (ASC-SH) and the pepsin-soluble collagen in the haruan scales (PSC-SH) were 1.44 and 2.94% (on a dry basis), respectively. ASC-SH and PSC-SH contained glycine as the major amino acid and had high imino acid contents (238 and 242 residues/1,000 residues, respectively). Based on the SDS-PAGE pattern, both ASC-SH and PSC-SH were identified as type I collagens containing α1 and α2 chains. β and γ components were also found in both collagens. The FTIR spectra indicated that both collagens had triple helical structures. The collagens were both soluble at acidic pH levels (1-4) and their solubility was low when the NaCl concentrations were above 3% (w/v). Conclusion: It was concluded that haruan scales could be an alternative source of collagen and that the characteristics of the collagens were slightly affected by the extraction process used in this study.
  Sri Supadmi , Agnes Murdiati and Endang Sutriswati Rahayu
  Background and Objectives: Mocaf reportedly contains a high content of amylose, which functions as an iodine carrier. Iodine is attached in a complex bond to its linear chain or to the branched chain of amylopectin. Evaluation of the absorption of iodine attached to amylose and amylopectin was performed to measure the amount of absorbed iodine in the small intestine. The absorption rate must be studied since iodine deficiency leads to health problems in all ages, especially affecting children’s growth and development. This study was designed to evaluate in vitro iodine absorption of potassium iodate (KIO3)-fortified mocaf in the small intestine. Materials and Methods: An in vitro evaluation of iodine absorption was conducted using the everted gut sac method. Data were statistically analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT). Results: The highest absorption occurred at 75 and 90 min, while the highest concentration was 40 ppm. The absorption rates at various concentrations were 56.23% (0 ppm), 65.53% (10 ppm), 69.29% (20 ppm), 71.91% (30 ppm) and 84.65% (40 ppm). The KIO3 concentration significantly affected iodine absorption (p≤0.05). Conclusion: The highest absorption rate was 89.10%. A higher KIO3 concentration increased iodine absorption and a longer absorption period tended to enhance the absorption rate.
  Welli Yuliatmoko , Agnes Murdiati , Yudi Pranoto and Yustinus Marsono
  Background and Objectives: Cavendish Jepara 30 (Musa cavendishii ) banana pseudostem in Indonesia is very abundant and has not been utilized because it is considered to be waste. The pseudostem contains dietary fibre, resistant starch (RS) and antioxidants that are strongly affected by several factors such as plant varieties and processing techniques. This study was conducted to investigate the effect of blanching and soaking in sodium bisulphite solution on changes in dietary fibre, resistant starch, antioxidants and the functional properties of Cavendish Jepara 30 banana pseudostem flour. Materials and Methods: Banana pseudostem of Cavendish Jepara 30 varieties were obtained from PT. Nusantara Tropical Farm Central Lampung, Indonesia. Preparation of the banana pseudostem flour included a blanching treatment at a temperature of 100°C for 10 min or soaking in 1% sodium bisulphite solution for 90 min. Results: The blanching treatment significantly increased the amount of soluble fibre by 3.55%, RS by 10.33%, total phenolic acids by 11.19 mg/100 g and antioxidant activity by 6.15% radical scavenging activity (RSA) but decreased the amount of insoluble fibre by 5.78%. The soaking treatment significantly increased the RS by 9.38% and the antioxidant activity by 12.5% RSA but decreased the insoluble fibre by 6.87%, the total fibre by 6.42% and the total phenolic acids by 17.52 mg/100 g. The blanching treatment also significantly increased the water holding capacity by10.81%, the swelling capacity by 13.87% and the cation exchange capacity was 9.75 meq kg–1. The microscopic structure of the flour after the blanching treatment was more porous and hollower than the natural pseudostem flour. Conclusion: Blanching treatment significantly increased the soluble fibre, RS, total phenolic acid content, antioxidant activity, water holding capacity, swelling capacity and cation exchange capacity of banana pseudostem flour. Blanching had no effect on the oil holding capacity of flour.
  Veriani Aprilia , Agnes Murdiati , Pudji Hastuti and Eni Harmayani
  Background and Objective: Probiotic is functional food gave good effect to human body if it is consumed in adequate amount. Its viability becomes lower during processing, storing and delivering to the body. Therefore, it needs to be protected. Hydrogel formed from interaction between glucomannan and chitosan may protect it. The aims of this study were to evaluate the characteristics of hydrogel made from glucomannan and chitosan and its efficiency in encapsulating Lactobacillus acidophilus FNCC 0051. Materials and Methods: Hydrogel was prepared by extruding 0.5% w/v chitosan in 1% acetic acid to 0.5% w/v carboxymethyl glucomannan. Properties of hydrogel such as particle size, morphology Fourier Transform Infra Red (FTIR) spectra and swelling ratio were measured. Encapsulation efficiencies were evaluated by enumerating encapsulated and unencapsulated cells. Data were analyzed using one way-ANOVA, then continued with Duncan’s Multiple Range Test (DMRT). Results: The result of the study showed that hydrogel formed using complex coacervation between glucomannan and chitosan had the spherical shape with the particle sizes around 1.09-2.31 μm. The new peak spectra at 1589 cm–1 confirmed that L. acidophilus was encapsulated in hydrogel matrix. The hydrogel respectively showed minimum and maximum swelling ratio at pH values of 5 and 8. Encapsulation efficiency was 66.1±3.0% when the cells were released in pH 8. It was not significantly different to the efficiency in pH 7. Enumerating unencapsulated cells provided lower encapsulation efficiency. Conclusions: L. acidophilus can be encapsulated in hydrogel using complex coacervation of glucomannan and chitosan. The low swelling ratio at lower pH provide better protection for the cell in the stomach, while the high swelling ratio at higher pH may be used to release the cell in small intestine. Further researches may be conducted to increase the encapsulation efficiency.
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