TY - JOUR T1 - Relationship between proteins encoded by three human gamma-crystallin genes and distinct polypeptides in the eye lens. JF - Molecular and Cellular Biology JO - Mol. Cell. Biol. SP - 3320 LP - 3323 DO - 10.1128/MCB.7.9.3320 VL - 7 IS - 9 AU - Russell, P AU - Meakin, S O AU - Hohman, T C AU - Tsui, L C AU - Breitman, M L Y1 - 1987/09/01 UR - http://mcb.asm.org/content/7/9/3320.abstract N2 - Although individual gamma-crystallins from the human eye lens have not been successfully purified and sequenced, most of the genes coding for these lens-specific structural proteins have been cloned and characterized. To investigate the relationship between these genes and the gamma-crystallins of the human lens, we made use of mouse cell lines which contain stably integrated copies of the coding sequences for three of the human gamma-crystallin genes coupled to the human metallothionein IIA promoter. The proteins produced by these hybrid genes in cell culture were detected immunologically and compared by physical characteristics with the gamma-crystallins from the human lens. The protein encoded by the G3 gene showed properties identical to those of the 21,000-molecular-weight gamma-crystallin from 11-month-old lens. The protein isolated from the cells expressing the G4 gene was similar to a 19,000-molecular-weight lens gamma-crystallin, while gene G5 encodes a highly basic gamma-crystallin which may be synthesized in only limited amounts in the human lens. These correlations provide a basis for future investigations on the relationship between putative mutations in human gamma-crystallin genes and altered proteins in hereditary lens cataracts. ER -