FRK Stable Knockout Human H1299 Cell Line

T95051x106 cells / 1.0 ml


DescriptionFRK Stable Knockout Human H1299 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the FRK gene (NM_002031) using the following guide RNA sequence: GAGGACTTGAGCTTCCGAGC. FRK gene has important functions during G1 and S phase of the cell cycle and thus can be used to study cell proliferation. This cell line is sequenced verified to be biallelic knockout.
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemAirway/Lung
Growth PropertiesAdherent
Cell MorphologyPolygonal
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Clone Name1C10
Cell TypeDrug Discovery Cells
Propagation Requirements
The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002 To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Selection: 0.5 μg/ml Puromycin (G264.)
Subculture Protocol1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new culture vessel. 6. Incubate cultures at 37°C.
2. Storage Temperature: Liquid nitrogen vapour phase.
Passage NumberP4
QC1) 0.5 µg/ml of puromycin (G264) was used for clonal selection; 2) FRK gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region









Supporting Protocol




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