IgG Screen Nutritional 88 ELISA Kit (DEIA302)


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serum, plasma
Species Reactivity
Intended Use
The IgG Screen Nutritional 88 ELISA test kit has been designed for the detection and the quantitative determination of specific food antigen related IgG antibodies in serum and plasma.
Contents of Kit
1. Microtiter Strips
2. Standards
3. Low positive Control
4. High positive Control
5. Sample Diluent
6. Conjugate
7. Substrate
8. Stop Solution
9. Washing Buffer
0.18 U/mL


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Customer Reviews



A compromised specific humoral immune response against the SARS-CoV-2 receptor-binding domain is related to viral persistence and periodic shedding in the gastrointestinal tract


Authors: Hu, Fengyu; Chen, Fengjuan; Ou, Zhihua; Fan, Qinghong; Tan, Xinghua; Wang, Yaping; Pan, Yuejun; Ke, Bixia; Li, Linghua; Guan, Yujuan; Mo, Xiaoneng; Wang, Jian; Wang, Jinlin; Luo, Chun; Wen, Xueliang; Li, Min; Ren, Peidi; Ke, Changwen; Li, Junhua; Lei, Chunliang; Tang, Xiaoping; Li, Feng

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been redetected after discharge in some coronavirus disease 2019 (COVID-19) patients. The reason for the recurrent positivity of the test and the potential public health concern due to this occurrence are still unknown. Here, we analyzed the viral data and clinical manifestations of 289 domestic Chinese COVID-19 patients and found that 21 individuals (7.3%) were readmitted for hospitalization after detection of SARS-CoV-2 after discharge. First, we experimentally confirmed that the virus was involved in the initial infection and was not a secondary infection. In positive retests, the virus was usually found in anal samples (15 of 21, 71.4%). Through analysis of the intracellular viral subgenomic messenger RNA (sgmRNA), we verified that positive retest patients had active viral replication in their gastrointestinal tracts (3 of 16 patients, 18.7%) but not in their respiratory tracts. Then, we found that viral persistence was not associated with high viral titers, delayed viral clearance, old age, or more severe clinical symptoms during the first hospitalization. In contrast, viral rebound was associated with significantly lower levels of and slower generation of viral receptor-binding domain (RBD)-specific IgA and IgG antibodies. Our study demonstrated that the positive retest patients failed to create a robust protective humoral immune response, which might result in SARS-CoV-2 persistence in the gastrointestinal tract and possibly in active viral shedding. Further exploration of the mechanism underlying the rebound in SARS-CoV-2 in this population will be crucial for preventing virus spread and developing effective vaccines.

IgG Immune Complexes Break Immune Tolerance of Human Microglia


Authors: van der Poel, Marlijn; Hoepel, Willianne; Hamann, Jorg; Huitinga, Inge; den Dunnen, Jeroen

Microglia are phagocytic cells involved in homeostasis of the brain and are key players in the pathogenesis of multiple sclerosis (MS). A hallmark of MS diagnosis is the presence of IgG Abs, which appear as oligoclonal bands in the cerebrospinal fluid. In this study, we demonstrate that myelin obtained post mortem from 8 out of 11 MS brain donors is bound by IgG Abs. Importantly, we show that IgG immune complexes strongly potentiate activation of primary human microglia by breaking their tolerance for microbial stimuli, such as LPS and Poly I:C, resulting in increased production of key proinflammatory cytokines, such as TNF and IL-1 beta. We identified Fc gamma RI and Fc gamma RIIa as the two main responsible IgG receptors for the breaking of immune tolerance of microglia. Combined, these data indicate that IgG immune complexes potentiate inflammation by human microglia, which may play an important role in MS-associated inflammation and the formation of demyelinating lesions.

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