FAQ: What is the difference between PNGase F and Endo F2?

PNGase F cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins. Whereas, Endo F2 has a high specificity for removing N-linked glycans within the chitobiose core of glycoproteins containing only biantennary and (at a reduced rate) high mannose oligosaccharides.

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Endo F2