Phosphotyrosine-binding domain (IRS1-like)
SMART accession number:SM00310
Description: -
Interpro abstract (IPR002404): Insulin receptor substrate-1 proteins contain both a pleckstrin homology domain (IPR001849) and a phosphotyrosine binding (PTB) domain. These domains facilitate interaction with the activated tyrosine-phosphorylated insulin receptor. The PTB domain is situated towards the N terminus. Two arginines in this domain are responsible for hydrogen bonding phosphotyrosine residues on a Ac-LYASSNPApY-NH2 peptide in the juxtamembrane region of the insulin receptor. Further interactions via `bridged' water molecules are coordinated by residues an Asn and a Ser residue [(PUBMED:8646778)].

The PTB domain has a compact, 7-stranded beta-sandwich structure, capped by a C-terminal helix. The substrate peptide fits into an L-shaped surface cleft formed from the C-terminal helix and strands 5 and 6 [(PUBMED:8599766)].

GO function:insulin receptor binding (GO:0005158)
Family alignment:
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There are 1280 PTBI domains in 1280 proteins in SMART's nrdb database.

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