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Luria-Bertani Medium (aka L-Broth or LB Medium). Standard growth medium for a variety of bacteria and conditions.


  1. 10 g Bacto-tryptone
  2. 5 g yeast extract
  3. 10 g NaCl


  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
    • 250°F, 22psi, 30 minutes



Adapted From:


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