Effects of DD concentration and time on NO accumulation.

NO accumulation in cells was measured using fluorescent dye DAF-FM diacetate by flow cytometry. MFI is Median Fluorescence Intensity. Unstained control and stained DMSO (0.1%) solvent control were used as negative controls. SNP (which is a NO donor) at 500 µM. acted as positive control. Error bars represent standard error. (*Significance with respect to DMSO solvent control calculated by student’s t-test, p<0.05, n = 3).