New Promega/MS Bioworks Pubhub article

Alternative proteases can improve mass spectrometry protein analysis by using unique digestion conditions or complimentary cleavage specificity. In this article, we use four alternative proteases: Arg-C, elastase, thermolysin and pepsin, and show typical applications for these proteases and how their use can improve mass spectrometry analysis. We demonstrate the advantages of these proteases using various model systems, including a yeast total protein extract, a post-translation modifcation-rich human histone H4, phosphorylase B and bacteriorhodopsin.


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