In vitro release profile of rhTGF-β3 from the EMLDS.

(A) Samples incubated in PBS were collected for 30 days and the amount of released rhTGF-β3 was examined using ELISA. Approximately 86% of the total rhTGF-β3 loaded was released from the EMLDS after 28 days. (B) The release rate of rhTGF-β3 was approximately 8 ng/day for the first five days and approximately 4 ng/day thereafter. The release profiles did not significantly differ when the amount of rhTGF-β3 loaded was varied from 50 ng to 200 ng.