MethoCult™ SF H4636

Serum-free methylcellulose-based medium with recombinant cytokines for human ES and iPS cell-derived cells

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418 USD


MethoCult™ SF H4636 is recommended for the culture of human embryonic stem (ES) cell-derived and induced pluripotent stem (iPS)
cell-derived hematopoietic progenitor cells in defined serum-free conditions. MethoCult™ SF H4636 is formulated to support optimal growth of erythroid progenitor cells (CFU-E and BFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G, CFU-M), and multipotential progenitor cells (CFU-GEMM; granulocyte, erythrocyte, macrophage, megakaryocyte).

MethoCult™ SF H4636 is also recommended for CFU assays with mononuclear cells, CD34+ enriched cells, and cells isolated by other purification methods from human bone marrow (BM), mobilized peripheral blood (MPB), peripheral blood (PB), and cord blood (CB) samples.
• Methylcellulose in Iscove's IMDM
• Bovine serum albumin
• 2-Mercaptoethanol
• Recombinant human insulin
• Human transferrin (iron-saturated)
• Cytokines including recombinant human erythropoietin (EPO)
• Supplements
Specialized Media; Semi-Solid Media
Cell Type:
Hematopoietic Stem and Progenitor Cells
Functional Assay; Cell Culture; Colony Assay
Area of Interest:
Stem Cell Biology

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Educational Materials


Frequently Asked Questions

Why use semi-solid media?


Why use methylcellulose-based media?


Is it necessary to add antibiotics to the media?


Is there anything I can do if my cultures appear contaminated?




Can I 'pluck' the colonies for individual analysis?


Why are low adherence dishes so important?


Can MethoCult™ products be used for lymphoid progenitor CFU assays?




Can I stain colonies in MethoCult™ medium?


Are there differences in colony morphology with serum-free media?


Can MethoCult™ be made with alternate base media?



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Product Applications


Data and Publications


Procedure Summary for Hematopoietic CFU Assays

Figure 1. Procedure Summary for Hematopoietic CFU Assays

Figure 2. Total Colony Numbers of CD34+ Cells Grown in MethoCult™ SF H4436 and MethoCult™ SF H4636

CD34+ and mononuclear cells derived from CB, mPB and BM were isolated and plated in MethoCult™ media and counted after 14 days. Shown are the number of total colonies grown in serum-free MethoCult™ SF H4436 (Catalog #04436) and MethoCult™ SF H4636 (Catalog #04636) normalized relative to total colonies grown in serum-containing MethoCult™ H4435 (Catalog #04435). MethoCult™ SF H4636 provides improved conditions for colony growth in serum-free conditions as compared to MethoCult™ SF H4436. Shown are means with standard deviation (n = 6).


Stem cell reports 2019 jun

Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives.

X. Cao et al.


A renewable source of human monocytes and macrophages would be a valuable alternative to primary cells from peripheral blood (PB) in biomedical research. We developed an efficient protocol to derive monocytes and macrophages from human induced pluripotent stem cells (hiPSCs) and performed a functional comparison with PB-derived cells. hiPSC-derived monocytes were functional after cryopreservation and exhibited gene expression profiles comparable with PB-derived monocytes. Notably, hiPSC-derived monocytes were more activated with greater adhesion to endothelial cells under physiological flow. hiPSC-derived monocytes were successfully polarized to M1 and M2 macrophage subtypes, which showed similar pan- and subtype-specific gene and surface protein expression and cytokine secretion to PB-derived macrophages. hiPSC-derived macrophages exhibited higher endocytosis and efferocytosis and similar bacterial and tumor cell phagocytosis to PB-derived macrophages. In summary, we developed a robust protocol to generate hiPSC monocytes and macrophages from independent hiPSC lines that showed aspects of functional maturity comparable with those from PB.