Acetylcholine release from the rabbit retina mediated by kainate receptors.

Article date: 1991/1/1

PubMed ID: 1670781

Journal name: The Journal of neuroscience : the official journal of the Society for Neuroscience (ISSN: 0270-6474)


The cholinergic amacrine cells of the rabbit retina may be labeled with 3H-choline (3H-Ch), and the activity of the cholinergic population may be monitored by following the release of 3H-ACh. Glutamate analogs caused massive ACh release, up to 50 times the basal efflux, with the following rank order of potency: alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) greater than quisqualate (QQ) = kainate (KA) much greater than NMDA (in magnesium-free medium) much greater than glutamate greater than aspartate. In contrast, the release of 3H-Ch was unchanged. Submaximal doses of each agonist were used to establish the specifity of glutamate antagonists. Kynurenic acid was selective for KA much greater than QQ, and 6,7-dinitroquinoxaline-2,3-dione (DNQX) was selective for KA greater than QQ much greater than NMDA. At low doses, which selectively blocked the response to KA, both antagonists blocked the light-evoked release of ACh. These results suggest that ACh release may be produced via several glutamate receptors, but the physiological input to the cholinergic amacrine cells is mediated by KA receptors. Because these cells receive direct input from cone bipolar cells, this work supports previous evidence that the bipolar cell transmitter is glutamate.

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Author List: Linn D M, Blazynski C, Redburn D A, Massey S C


Substances mentioned in the article: Glutamates; Receptors, Kainic Acid; Receptors, Neurotransmitter; Ibotenic Acid; Aspartic Acid; Glutamic Acid; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Choline; Acetylcholine; Kainic Acid;

Mesh terms: Acetylcholine/metabolism; Animals; Aspartic Acid/pharmacology; Choline/metabolism; Glutamates/pharmacology; Glutamic Acid; Ibotenic Acid/analogs & derivatives; In Vitro Techniques; Kainic Acid/metabolism; N-Methylaspartate/pharmacology; Quisqualic Acid/pharmacology; Rabbits; Receptors, Kainic Acid; Receptors, Neurotransmitter/drug effects; Retina/drug effects; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;
