TY - JOUR TI - Measurement of NADPH Oxidase Activity in Plants AU - Kaundal, Amita AU - Rojas, Clemencia M. AU - Mysore, Kirankumar S. VL - 2 IS - 20 PY - 2012 DA - 2012/10/20 SP - e278 C1 - Bio-protocol 2012;2:e278 DO - 10.21769/BioProtoc.278 UR - https://doi.org/10.21769/BioProtoc.278 AB - NADPH oxidase is a membrane-bound enzyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2-is spontaneously dismasted to the more stable form H2O2. Both O2-and H2O2 are forms ofreactive oxygen species (ROS), which are involved in regulation of many cellular activities such as transcription, intracellular signaling, and host defense. The NADPH oxidase - dependent generation of O2- in total membrane fraction of plant tissue has been determined by the reduction of the tetrazolium salt XTT by O2-. In the presence of O2-, XTT generates a soluble yellow formazan that can be quantified spectrophotometrically. JF - Bio-protocol SN - 2331-8325 PB - Bio-protocol LLC.