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Technology Laboratory Research & Design

JM Material Technology Nanotechnology in collaboration with the Stem Cell clinical research center tested JM's nano composite material against viruses using the following tests:


Using a transparent spot forming region (Plaque Forming) method, after exposure to JM nano composite material the sample test was cultured to test for residual virus.
Virus Type:
1. Enterovirus: Enterovirus echovirus type 11 , from the College of American Pathologists Proficiency Test strains.
2. Influenza A virus : Influenza A virus-New Caledonia / 20/99 (H1N1), from the College of American Pathologists Proficiency Test strains.
Host cell:
1.A type influenza viruses using MDCK cell lines (BCRC 60004).
2. Enterovirus using LLC-MK2 cell line (BCRC 60092).
JM nano composite material TTA anti-virus test:
The test mainly focuses on virus enterovirus species and Influenza A virus, after cells are infected with the virus, the cells die (as shown below), the effect of cell enzyme activity on the receptor was tested by MTT crystal staining in order to analyse the cell survival rate and determine the antiviral rate, when testing the material for antiviral effectiveness the higher anti viral rate the higher cell viability, but due to differences in viruses and bacteria it can not be measured directly, therefore cells are infected using plaque formation and uses the virus plaque forming units (PFU; plaque-forming unit) to calculate and determine the concentration of virus in units of PFU / Ml.

From the figure it can be clearly seen when cells are infected with the virus died, crystallization by MTT staining pattern can be seen in the differences of living cells and dead cells.

The following is not added to the case for any of the material, when inoculated with different viruses cell viability test.

According to the anti-viral tests above, in accordance with US FDA certified laboratory MICROBIOTEST for antiviral use certification standard ASTM E 1052-96, and methods in collaboration with Stem Cell Clinical and Medical Research Center to test and calculate the viral suppression ability inhibiting viral efficacy.
1. 2 × 106 PFU / mL concentration of virus testing, JM material LLC-MK2 cells inhibit the effect of enterovirus infection, inhibiting ability reaching 99.99%.
2. 4 × 103 PFU / mL concentration of virus testing, JM materials inhibit the A-type virus infected MDCK cells effect, inhibiting ability reaching 99.99%.




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