This is a continuation of application Ser. No. 08/774,736, filed Jan. 2, 1997 now U.S. Pat. No. 6,261,762 which is a division of 07/810,908, filed Dec. 20, 1991, which is a division of 07/752,368, filed Sep. 3, 1991 now abandoned, which is a division of 07/013,477, filed Feb. 11, 1987 now U.S. Pat. No. 5,079,342, which is a division of 07/003,764, filed Jan. 16, 1981 now U.S. Pat. No. 5,051,496, which is a CIP of 06/933,184, filed Nov. 21, 1986 now abandoned, which is a CIP of 06/916,080, filed Oct. 6, 1986 now abandoned, which is a CIP of 06/835,228, filed Mar. 3, 1986 now U.S. Pat. No. 4,839,288, which is a CIP based on FR 86 00911, filed on Jan. 22, 1986, based on FR 86 01635, filed on Feb. 6, 1986, based on FR 86 01985, filed Feb. 13, 1986, based on FR 86 03881, filed on Mar. 18, 1986, based on FR 86 04215, filed on Mar. 24 1986, incorporated herein by reference. 
    
    
     BACKGROUND OF THE INVENTION 
     The invention relates to cloned DNA sequences analogous to the genomic RNA of a virus known as Lymphadenopathy-Associated Virus II (“LAV-II”), a process for the preparation of these cloned DNA sequences, and their use as probes in diagnostic kits. In one embodiment, the invention relates to a cloned DNA sequence analogous to the entire genomic RNA of HIV-2 and its use as a probe. The invention also relates to polypeptides with amino acid sequences encoded by these cloned DNA sequences and the use of these polypeptides in diagnostic kits. 
     According to recently adopted nomenclature, as reported in Nature, May 1986, a substantially-identical group of retroviruses which has been identified as one causative agent of AIDS are now referred to as Human Immunodeficiency Viruses I (HIV-1). This previously-described group of retroviruses includes Lymphadenopathy-Associated Virus I (LAV-I), Human T-cell Lymphotropic Virus-III (HTLV-III), and AIDS-Related Virus (ARV). 
     Lymphadenopathy-Associated Virus II has been described in U.S. application Ser. No. 835,228, which was filed Mar. 3, 1986, and is specifically incorporated herein by reference. Because LAV-II is a second, distinct causative agent of AIDS, LAV-II properly is classifiable as a Human Immunodeficiency Virus II (HIV-2). Therefore, “LAV-II” as used hereinafter describes a particular genus of HIV-2 isolates. 
     While HIV-2 is related to HIV-1 by its morphology, its tropism and its in vitro cytopathic effect on CD4 (T4) positive cell lines and lymphocytes, HIV-2 differs from previously described human retroviruses known to be responsible for AIDS. Moreover, the proteins of HIV-1 and 2 have different sizes and their serological cross-reactivity is restricted mostly to the major core protein, as the envelope glycoproteins of HIV-2 are not immune precipitated by HIV-1-positive sera except in some cases where very faint cross-reactivity can be detected. Since a significant proportion of the HIV infected patients lack antibodies to the major core protein of their infecting virus, it is important to include antigens to both HIV-1 and HIV-2 in an effective serum test for the diagnosis of the infection by these viruses. 
     HIV-2 was first discovered in the course of serological research on patients native to Guinea-Bissau who exhibited clinical and immunological symptoms of AIDS and from whom sero-negative or weakly sero-positive reactions to tests using an HIV-1 lysate were obtained. Further clinical studies on these patients isolated viruses which were subsequently named “LAV-II.” 
     One LAV-II isolate, subsequently referred to as LAV-II MIR, was deposited at the Collection Nationale des Cultures de Micro-Organismes (CNCM) at the Institut Pasteur in Paris, France on Dec. 19, 1985 under Accession No. I-502 and has also been deposited at the British ECA CC under No. 87.001.001 on Jan. 9, 1987. A second LAV-II isolate was deposited at CNCM on Feb. 21, 1986 under Accession No. I-532 and has also been deposited at the British ECA CC under No. 87.001.002 on Jan. 9, 1987. This second isolate has been subsequently referred to as LAV-II ROD. Other isolates deposited at the CNCM on Dec. 19, 1986 are HIV-2 IRMO (No. I-642) and HIV-2 EHO (No. I-643). Several additional isolates have been obtained from West African patients, some of whom have AIDS, others with AIDS-related conditions and others with no AIDS symptoms. All of these viruses have been isolated on normal human lymphocyte cultures and some of them were thereafter propagated on lymphoid tumor cell lines such as CEM and MOLT. 
     Due to the sero-negative or weak sero-positive results obtained when using kits designed to identify HIV-1 infections in the diagnosis of these new patients with HIV-2 disease, it has been necessary to devise a new diagnostic kit capable of detecting HIV-2 infection, either by itself or in combination with an HIV-1 infection. The present inventors have, through the development of cloned DNA sequences analogous to at least a portion of the genomic RNA of LAV-II ROD viruses, created the materials necessary for the development of such kits. 
     SUMMARY OF THE INVENTION 
     As noted previously, the present invention relates to the cloned nucleotide sequences homologous or identical to at least a portion of the genomic RNA of HIV-2 viruses and to polypeptides encoded by the same. The present invention also relates to kits capable of diagnosing an HIV-2 infection. 
     Thus, a main object of the present invention is to provide a kit capable of diagnosing an infection caused by the HIV-2 virus. This kit may operate by detecting at least a portion of the RNA genome of the HIV-2 virus or the provirus present in the infected cells through hybridization with a DNA probe or it may operate through the immunodiagnostic detection of polypeptides unique to the HIV-2 virus. 
     Additional objects and advantages of the present invention will be set forth in part in the description which follows, or may be learned from practice of the invention. The objects and advantages may be realized and attained by means of the instrumentalities and combinations particularly pointed out in the appended claims. 
     To achieve these objects and in accordance with the purposes of the present invention, cloned DNA sequences related to the entire genomic RNA of the LAV-II virus are set forth. These sequences are analogous specifically to the entire genome of the LAV-II ROD strain. 
     To further achieve the objects and in accordance with the purposes of the present invention, a kit capable of diagnosing an HIV-2 infection is described. This kit, in one embodiment, contains the cloned DNA sequences of this invention which are capable of hybridizing to viral RNA or analogous DNA sequences to indicate the presence of an HIV-2 infection. Different diagnostic techniques can be used which include, but are not limited to: (1) Southern blot procedures to identify viral DNA which may or may not be digested with restriction enzymes; (2) Northern blot techniques to identify viral RNA extracted from cells; and (3) dot blot techniques, i.e., direct filtration of the sample through an ad hoc membrane such as nitrocellulose or nylon without previous separation on agarose gel. Suitable material for dot blot technique could be obtained from body fluids including, but not limited to, serum and plasma, supernatants from culture cells, or cytoplasmic extracts obtained after cell lysis and removal of membranes and nuclei of the cells by ultra-centrifugation as accomplished in the “CYTODOT” procedure as described in a booklet published by Schleicher and Schull. 
     In an alternate embodiment, the kit contains the polypeptides created using these cloned DNA sequences. These polypeptides are capable of reacting with antibodies to the HIV-2 virus present in sera of infected individuals, thus yielding an immunodiagnostic complex. 
     To further achieve the objects of the invention, a vaccinating agent is provided which comprises at least one peptide selected from the polypeptide expression products of the viral DNA in admixture with suitable carriers, adjuvents stabilizers. 
     It is understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention as claimed. The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate one embodiment of the invention and, together with the description, serve to explain the principles of the invention. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 generally depicts the nucleotide sequence of a cloned complementary DNA (cDNA) to the genomic RNA of HIV-2. FIG. 1A depicts the genetic organization of HIV-1, position of the HIV-1 HindIII fragment used as a probe to screen the cDNA library, and restriction map of the HIV-2 cDNA clone, E2. FIG. 1B depicts the nucleotide sequence of the 3′ end of HIV-2. The corresponding region of the HIV-1 LTR was aligned using the Wilbur and Lipman algorithm (window: 10; K-tuple: 7; gap penalty: 3) as described by Wilbur and Lipman in Proc. Natl. Acad. Sci. USA 80: 726-730 (1983), specifically incorporated herein by reference. The U3-R junction in HIV-1 is indicated and the poly A addition signal and potential TATA promoter regions are boxed. In FIG. 1B, the symbols B, H, Ps and Pv refer to the restriction sites BamHI, HindIII, PstI and PvuII, respectively. 
     FIG. 2 generally depicts the HIV-2 specificity of the E2 clone. FIGS. 2A and B specifically depict a Southern blot of DNA extracted from CEM cells infected with the following isolates: HIV-2 ROD  (a,c), HIV-2 DUL  (b,d), and HIV-1 BRU  (e,f). DNA in lanes a, b, f was Pst I digested; in c, d, e DNA was undigested. FIGS. 2C and D specifically depict dot blot hybridization of pelleted virions from CEM cells infected by the HIV-1 BRU  (1), Simian Immunodeficiency Virus (SIV) isolate Mm 142-83 (3), HIV-2 DUL  (4), HIV-2 ROD  (5), and HIV-1 ELI  (6). Dot 2 is a pellet from an equivalent volume of supernatant from uninfected CEM. Thus, FIGS. 2A and C depicts hybridization with the HIV-2 cDNA (E2) and FIGS. 2B and D depicts hybridization to an HIV-1 probe consisting of a 9 Kb Sacd insert from HIV-1 BRU(clone lambda J 19). 
     FIG. 3 generally depicts a restriction map of the HIV-2 ROD genome and its homology to HIV-1. FIG. 3A specifically depicts the organization of three recombinant phage lambda clones, ROD 4, ROD 27, and ROD 35. In FIG. 3A, the open boxes represent viral sequences, the LTR are filled, and the dotted boxes represent cellular flanking sequences (not mapped). Only some characteristic restriction enzyme sites are indicated. λROD 27 and ROD 35 are derived from integrated proviruses while λROD 4 is derived from a circular viral DNA. The portion of the lambda clones that hybridzes to the cDNA E2 is indicated below the maps. A restriction map of the λROD isolate was reconstructed from these three lambda clones. In this map, the restriction sites are identified as follows: B: BamHI; E: EcoRI; H: HindIII; K: KpnI; Ps: PstI; Pv: PvuII; S: SacI; X: XbaI. R and L are the right and left BamHI arms of the lambda L47.1 vector. 
     FIG. 3B specifically depicts dots 1-11 which correspond to the single-stranded DNA form of M13 subclones from the HIV-1 BRU  cloned genome (λJ19). Their size and position on the HIV-1 genome, determined by sequencing is shown below the figure. Dot 12 is a control containing lambda phage DNA. The dot-blot was hybridized in low stringency conditions as described in Example 1 with the complete lambda λROD 4 clone as a probe, and successively washed in 2×SSC, 0.1% SDS at 25° C. (Tm−42° C.),1×SSC, 0.1% SDS at 60° C. (Tm −20° C.), and 0.1×SSC, 0.1% SDS at 60° C. (Tm −3° C.) and exposed overnight. A duplicate dot blot was hybridized and washed in stringent conditions (as described in Example 2) with the labelled lambda J19 clone carrying the complete HIV-1 BRU  genome. HIV-1 and HIV-2 probes were labelled the same specific activity (10 8  cpm/g.). 
     FIG. 4 generally depicts the restriction map polymorphism in different HIV-2 isolates and shows comparison of HIV-2 to SIV. FIG. 4A specifically depicts DNA (20 ug. per lane) from CEM cells infected by the isolate HIV-2 DUL  (panel 1) or peripheral blood lymphocytes (PBL) infected by the isolates HIV-2 GOM  (panel 2) and HIV-2 MIR  (panel 3) digested with: EcoRI (a), PstI (b), and HindIII (c). Much less viral DNA was obtained with HIV-2 isolates propagated on PBL. Hybridization and washing were in stringent conditions, as described in Example 2, with 106 cpm/ml. of each of the E2 insert (cDNA) and the 5 kb. HindIII fragment of λROD 4, labelled to 10 9  cpm/ug. 
     FIG. 4B specifically depicts DNA from HUT 78 (a human T lymphoid cell line) cells infected with STLV3 MAC isolate Mm 142-83. The same amounts of DNA and enzymes were used as indicated in panel A. Hybridization was performed with the same probe as in A, but in non-stringent conditions. As described in Example 1 washing was for one hour in 2×SSC, 0.1% SDS at 40° C. (panel 1) and after exposure, the same filter was re-washed in 0.1×SSC, 0.1% SDS at 60° C. (panel 2). The autoradiographs were obtained after overnight exposition with intensifying screens. 
     FIG. 5 depicts the position of derived plasmids from λROD 27, λROD 35 and λROD 4. 
    
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     Reference will now be made in detail to the presently preferred embodiments of the invention, which, together with the following examples, serve to explain the principles of the invention. 
     The genetic structure of the HIV-2 virus has been analyzed by molecular cloning according to the method set forth herein and in the Examples. A restriction map of the genome of this virus is included in FIG.  4 . In addition, the partial sequence of a cDNA complementary to the genomic RNA of the virus has been determined. This cDNA sequence information is included in FIG.  1 . 
     Also contained herein is data describing the molecular cloning of the complete 9.5 kb genome of HIV-2, data describing the observation of restriction map polymorphism between different isolates, and an analysis of the relationship between HIV-2 and other human and simian retroviruses. From the totality of these data, diagnostic probes can be discerned and prepared. 
     Generally, to practice one embodiment of the present invention, a series of filter hybridizations of the HIV-2 RNA genome with probes derived from the complete cloned HIV-1 genome and from the ca and Dol genes were conducted. These hybridizations yielded only extremely weak signals even in conditions of very low stringency of hybrization and washing. Thus, it was found to be difficult to assess the amount of HIV-2 viral and proviral DNA in infected cells by Southern blot techniques. 
     Therefore, a complementary DNA (cDNA) to the HIV-2 genomic RNA initially was cloned in order to provide a specific hybridization probe. To construct this cDNA, an oligo (dT) primed cDNA first-strand was made in a detergent-activated endogenous reaction using HIV-2 reverse transcriptase with virions purified from supernatants of infected CEM cells. The CEM cell line is a lymphoblastoid CD4+ cell line described by G. E. Foley et al. in Cancer 18: 522-529 (1965), specifically incorporated herein by reference. The CEM cells used were infected with the isolate ROD and were continuously producing high amounts of HIV-2. 
     After second-strand synthesis, the cDNAs were inserted into the M 13 tg 130 bacteriophage vector. A collection of 10 4  M13 recombinant phages was obtained and screened in situ with an HIV-1 probe spanning 1.5 kb. of the 3′ end of the LAV BRU  isolate (depicted in FIG.  1 A). Some 50 positive plaques were detected, purified, and characterized by end sequencing and cross-hybridizing the inserts. This procedure is described in more detail in Example 1 and in FIG.  1 . 
     The different clones were found to be complementary to the 3′ end of a polyadenylated RNA having the AATAAA signal about 20 nucleotides upstream of the poly A tail, as found in the long terminal repeat (LTR) of HIV-1. The LTR region of HIV-1 has been described by S. Wain Hobson et al. in Cell 40: 9-17 (1985), specifically incorporated herein by reference. The portion of the HIV-2 LTR that was sequenced was related only distantly to the homologous domain in HIV-1 as demonstrated in FIG.  1 B. Indeed, only about 50% of the nucleotides could be aligned and about a hundred insertions/deletions need to be introduced. In comparison, the homology of the corresponding domains in HIV-1 isolates from USA and Africa is greater than 95% and no insertions or deletions are seen. 
     The largest insert of this group of M13 clones was a 2 kb. clone designated E2. Clone E2 was used as a probe to demonstrate its HIV-2 specificity in a series of filter hybridization experiments. Firstly, this probe could detect the genomic RNA of HIV-2 but not HIV-1 in stringent conditions as shown in FIG. 2, C and D. Secondly, positive signals were detected in Southern blots of DNA from cells infected with the ROD isolate as well as other isolates of HIV-2 as shown in FIG. 2, A and FIG. 4, A. No signal was detected with DNA from uninfected cells or HIV-1 infected cells, confirming the exogenous nature of HIV-2. In undigested DNA from HIV-2 infected cells, an approximately 10 kb. species, probably corresponding to linear unintegrated viral DNA, was principally detected along with a species with an apparent size of 6 kb., likely to be the circular form of the viral DNA. Conversely, rehybridization of the same filter with an HIV-1 probe under stringent conditions showed hybridization to HIV-1 infected cells only as depicted in FIG. 2, B. 
     To isolate the remainder of the genome of HIV-2, a genomic library in lambda phage L47.1 was constructed. Lambda phage L47.1 has been described by W. A. M. Loenen et al. in Gene 10: 249-259 (1980), specifically incorporated herein by reference. The genomic library was constructed with a partial Sau3AI restriction digest of the DNA from the CEM cell line infected with HIV-2 ROD . 
     About 2×10 6  recombinant plaques were screened in situ with labelled insert from the E2 cDNA clone. Ten recombinant phages were detected and plaque purified. Of these phages, three were characterized by restriction mapping and Southern blot hybridization with the E2 insert and probes from its 3′ end (LTR) or 5′ end (envelope), as well as with HIV-1 subgenomic probes. In this instance, HIV-1 probes were used under non-stringent conditions. 
     A clone carrying a 9.5 kb. insert and derived from a circular viral DNA was identified as containing the complete genome and designated λROD 4. Two other clones, λROD 27 and λROD 35 were derived from integrated proviruses and found to carry an LTR and cellular flanking sequences and a portion of the viral coding sequences as shown in FIG. 3, A. 
     Fragments of the lambda clones were subcloned into a plasmid vector p UC 18. 
     Plasmid pROD 27-5′ is derived from λROD 27 and contains the 5′ 2Kb of the HIV-2 genome and cellular flanking sequences (5′ LTR and 5′ viral coding sequences to the EcoRI site) 
     Plasmid p ROD 4-8 is dervied from λROD 4 and contains the about 5 Kb HindIII fragment that is the central part of the HIV-2 genome. 
     Plasmid pROD 27-5′ and p ROD 4.8 inserts overlap. 
     Plasmid pROD 4.7 contains a HindIII 1.8 Kb fragment from λROD 4. This fragment is located 3′ to the fragment subcloned into pROD 4.8 and contains about 0.8 Kb of viral coding sequences and the part of the lambda phage (λL47.1) left arm located between the BamHI and HindIII cloning sites. 
     Plasmid pROD 35 contains all the HIV-2 coding sequences 3′ to the EcoRI site, the 3′ LTR and about 4 Kb of cellular flanking sequences. 
     Plasmid pROD 27-5′ and pROD 35 in  E. coli  strain HB 101 are deposited respectively under No. 1-626 and 1-633 at the CNCM, and have also been deposited at the NCIB (British Collection). These plasmids are depicted in FIG.  5 . Plasmids pROD 4-7 and pROD 4-8 in  E. coli  strain TGI are deposited respectively under No. 1-627 and 1-628 at the CNCM. 
     To reconstitute the complete HIV-2 ROD genome, pROD 35 is linearized with EcoRI and the EcoRI insert of pROD 27-5′ is ligated in the correct orientation into this site. 
     The relationship of HIV-2 to other human and simian retroviruses was surmised from hybridization experiments. The relative homology of the different regions of the HIV-1and 2 genomes was determined by hybridization of fragments of the cloned HIV-1 genome with the labelled λROD 4 expected to contain the complete HIV-2 genome (FIG. 3, B). Even in very low stringency conditions (Tm−42° C.), the hybridization of HIV-1 and 2 was restricted to a fraction of their genomes, principally the qaq gene (dots 1 and 2), the reverse transcriptase domain in pol (dot 3), the end of pol and the Q (or sor) genes (dot 5) and the F gene (or 3′ orf) and 3′ LTR (dot 11). The HIV-1 fragment used to detect the HIV-2 cDNA clones contained the dot 11 subclone, which hybridized well to HIV-2 under non-stringent conditions. Only the signal from dot 5 persisted after stringent washing. The envelope gene, the region of the tat gene and a part of pol thus seemed very divergent. These data, along with the LTR sequence obtained (FIG. 1, B), indicated that HIV-2 is not an envelope variant of HIV-1, as are African isolates from Zaire described by Alizon et al.,  Cell  40: 63-74 (1986). 
     It was observed that HIV-2 is related more closely to the Simian Immunodeficiency Virus (SIV) than it is to HIV-1. This correlation has been described by F. Clavel et al. in C. R. Acad. Sci. (Paris) 302: 485-488 (1986) and F. Clavel et al. in Science 233: 343-346 (1986), both of which are specifically incorporated herein by reference. Simian Immunodeficiency Virus (also designated Simian T-cell Lymphotropic Virus Type 3, STLV-3) is a retrovirus first isolated from captive macaques with an AIDS-like disease in the USA. This simian virus has been described by M.D. Daniel et al. in Science 228: 1201-1204 (1985), specifically incorporated herein by reference. 
     All the SIV proteins, including the envelope, are immune precipitated by sera from HIV-2 infected patients, whereas the serological cross-reactivity of HIV-l to 2 is restricted to the core proteins. However SIV and HIV-2 can be distinguished by slight differences in the apparent molecular weight of their proteins. 
     In terms of nucleotide sequence, it also appears that HIV-2 is closely related to SIV. The genomic RNA of SIV can be detected in stringent conditions as shown in FIG. 2, C by HIV-2 probes corresponding to the LTR and 3′ end of the genome (E2) or to the qaq or pol genes. Under the same conditions, HIV-1 derived probes do not detect the SIV genome as shown in FIG. 2, D. 
     In Southern blots of DNA from SIV-infected cells, a restriction pattern clearly different from HIV-2 ROD  and other isolates is seen. All the bands persist after a stringent washing, even though the signal is considerably weakened, indicating a sequence homology throughout the genomes of HIV-2 and SIV. It has recently been shown that baboons and macaques could be infected experimentally by HIV-2, thereby providing an interesting animal model for the study of the HIV infection and its preventive therapy. Indeed, attempts to infect non-human primates with HIV-1 have been successful only in chimpanzees, which are not a convenient model. 
     From an initial survey of the restriction maps for certain of the HIV-2 isolates obtained according to the methods described herein, it is already apparent that HIV-2, like HIV-1, undergoes restriction site polymorphism. FIG. 4 A depicts examples of such differences for three isolates, all different one from another and from the cloned HIV-2 ROD . It is very likely that these differences at the nucleotide level are accompanied by variations in the amino-acid sequence of the viral proteins, as evidenced in the case of HIV-1 and described by M. Alizon et al. in Cell 46: 63-74 (1986), specifically incorporated herein by reference. It is also to be expected that the various isolates of HIV-2 will exhibit amino acid heterogeneities. See, for example, Clavel et al.,  Nature  324 (18):691-695 (1986), specifically incorporated herein by reference. 
     Further, the characterization of HIV-2 will also delineate the domain of the envelope glycoprotein that is responsible for the binding of the surface of the target cells and the subsequent internalization of the virus. This interaction was shown to be mediated by the CD4 molecule itself in the case of HIV-1 and similar studies tend to indicate that HIV-2 uses the same receptor. Thus, although there is wide divergence between the env genes of HIV-1 and 2, small homologous domains of the envelopes of the two HIV could represent a candidate receptor binding site. This site could be used to raise a protective immune response against this group of retroviruses. 
     From the data discussed herein, certain nucleotide sequences have been identified which are capable of being used as probes in diagnostic methods to obtain the immunological reagents necessary to diagnose an HIV-2 infection. In particular, these sequences may be used as probes in hybridization reactions with the genetic material of infected patients to indicate whether the RNA of the HIV-2 virus is present in these patient&#39;s lymphocytes or whether an analogous DNA is present. In this embodiment, the test methods which may be utilized include Northern blots, Southern blots and dot blots. One particular nucleotide sequence which may be useful as a probe is the combination of the 5 kb. HindIII fragment of ROD 4 and the E2 cDNA used in FIG.  4 . 
     In addition, the genetic sequences of the HIV-2 virus may be used to create the polypeptides encoded by these sequences. Specifically, these polypeptides may be created by expression of the cDNA obtained according to the teachings herein in hosts such as bacteria, yeast or animal cells. These polypeptides may be used in diagnostic tests such as immunofluorescence assays (IFA), radioimmunoassays. (RIA) and Western Blot tests. 
     Moreover, it is also contemplated that additional diagnostic tests, including additional immunodiagnostic tests, may be developed in which the DNA probes or the polypeptides of this invention may serve as one of the diagnostic reagents. The invention described herein includes these additional test methods. 
     In addition, monoclonal antibodies to these polypeptides or fragments thereof may be created. The monoclonal antibodies may be used in immunodiagnostic tests in an analogous manner as the polypeptides described above. 
     The polypeptides of the present invention may also be used as immunogenic reagents to induce protection against infection by HIV-2 viruses. In this embodiment, the polypeptides produced by recombinant-DNA techniques would function as vaccine agents. 
     Also, the polypeptides of this invention may be used in competitive assays to test the ability of various antiviral agents to determine their ability to prevent the virus from fixing on its target. 
     Thus, it is to be understood that application of the teachings of the present invention to a specific problem or environment will be within the capabilities of one having ordinary skill in the art in light of the teachings contained herein. Examples of the products of the present invention and representative processes for their isolation and manufacture appear above and in the following examples. 
     EXAMPLES 
     Example 1 
     Cloning of a cDNA Complementary to Genomic RNA From HIV-2 Virions 
     HIV-2 virions were purified from 5 liters of supernatant from a culture of the CEM cell line infected with the ROD isolate and a cDNA first strand using oligo (dT) primer was synthesized in detergent activated endogenous reaction on pelleted virus, as described by M. Alizon et al. in Nature, 312: 757-760 (1984), specifically incorporated herein by reference. RNA-cDNA hybrids were purified by phenol-chloroform extraction and ethanol precipitation. The second-strand cDNA was created by the DNA polymerase I/RNAase H method of Gubler and Hoffman in Gene, 25: 263-269 (1983), specifically incorporated herein by reference, using a commercial cDNA synthesis kit obtained from Amersham. After attachment of EcoRI linkers (obtained from Pharmacia), EcoRI digestion, and ligation into EcoRI-digested dephosphorylated M13 tg 130 vector (obtained from Amersham), a cDNA library was obtained by transformation of the  E. coli  TGl strain. Recombinant plaques (104) were screened in situ on replica filters with the 1.5 kb. HindIII fragment from clone J19, corresponding to the 3′ part of the genome of the LAVBRU isolate of HIV-1,  32 p labelled to a specific activity of 10 9 cpm ug. The filters were prehybridized in 5×SSC, 5×Denhardt solution, 25% formamide, and denatured salmon sperm DNA (100 ug/ml.) at 37° C. for 4 hours and hybridized for 16 hours in the same buffer (Tm −42° C.) plus 4×10 7  cpm of the labelled probe (10 6  cpm/ml. of hybridization buffer). The washing was done in 5×SSC, 0.1% SDS at 25° C. for 2 hours. 20×SSC is 3 M NaCl, 0.3 M Na citrate. Positive plaques were purified and single-stranded M13 DNA prepared and end-sequenced according to the method described in Proc. Nat&#39;l. Acad. Sci. USA, 74: 5463-5467 (1977) of Sanger et al. 
     Example 2 
     Hybridization of DNA from HIV-1 and HIV-2 Infected Cells and RNA from HIV-1 and 2 and SIV Virons With a Probe Derived From an HIV-2 Cloned cDNA 
     DNA was extracted from infected CEM cells continuously producing HIV-1 or 2. The DNA digested with 20 ug of PstI digested with or undigested, was electrophoresed on a 0.8% agarose gel, and Southern-transferred to nylon membrane. Virion dot-blots were prepared in duplicate, as described by F. Clavel et al. in Science 233: 343-346 (1986), specifically incorporated herein by reference, by pelleting volumes of supernatant corresponding to the same amount of reverse transcriptase activity. Prehybridization was done in 50% formamide, 5×SSC, 5×Denhardt solution, and 100 mg./ml. denatured salmon sperm DNA for 4 hours at 42° C. Hybridization was performed in the same buffer plus 10% Dextran sulphate, and 10 6  cpm/ml. of the labelled E2 insert (specific activity 10 9  cpm/ug.) for 16 hours at 42° C. Washing was in 0.1×SSC, 0.1% SDS for 2 ×30 mn. After exposition for 16 hours with intensifying screens, the Southern blot was dehybridized in 0.4 N NaOH, neutralized, and rehybridized in the same conditions to the HIV-1 probe labelled to 10 9  cpm/ug. 
     Example 3 
     Cloning in Lambda Phage of the Complete Provirus DNA of HIV-2 
     DNA from the HIV-2 ROD  infected CEM (FIG. 2, lanes a and c) was partially digested with Sau3AI. The 9-15 kb. fraction was selected on a 5-40% sucrose gradient and ligated to BamHI arms of the lambda L47.1 vector. Plaques (2×106) obtained after in vitro packaging and plating on  E. coli  LA 101 strain were screened in situ with the insert from the E2 cDNA clone. Approximately 10 positive clones were plaque purified and propagated on  E. coli  C600 recBC. The ROD 4, 27, and 35 clones were amplified and their DNA characterized by restriction mapping and Southern blotting with the HIV-2 cDNA clone under stringent conditions, and gag-pol probes from HIV-1 used under non stringent conditions. 
     Example 4 
     Complete Genomic Sequence of the ROD HIV-2 Isolate 
     Experimental analysis of the HIV-2 ROD isolate yielded the following sequence which represents the complete genome of this HIV-2 isolate. Genes and major expression products identified within the following sequence are indicated by nucleotides numbered below: 
     1) GAG gene (546-2111) expresses a protein product having a molecular weight of around 55Kd and is cleaved into the following proteins: 
     a) p 16 (546-950) 
     b) p 26 (951-1640) 
     c) p 12 (1701-2111) 
     2) polymerase (1829-4936) 
     3) Q protein (4869-5513) 
     4) R protein (5682-5996) 
     5) X protein (5344-5679) 
     6) Y protein (5682-5996) 
     7) Env protein (6147-8720) 
     8) F protein (8557-9324) 9) TAT gene (5845-6140 and 8307-8400) is expressed by two exons separated by introns. 
     10) ART protein (6071-6140 and 8307-8536) is similarly the expression product of two exons. 
     11) LTR:R (1-173 and 9498-9671) 
     12) U5 (174-299) 
     13) U3 (8942-9497) 
     It will be known to one of skill in the art that the absolute numbering which has been adopted is not essential. For example, the nucleotide within the LTR which is designated as “1” is a somewhat arbitrary choice. What is important is the sequence information provided. 
     
       
         
               
             
           
               
                 GGTCGCTCTGCGGAGAGGCTGGCAGATTGAGCCCTGGGAGGTTCTCTCCAGCACTAGCAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 GTAGAGCCTGGGTGTTCCCTGCTAGACTCTCACCAGCACTTGGCCGGTGCTGGGCAGACG 
               
               
                          *         *         *       100         *         * 
               
               
                   
               
               
                 GCCCCACGCTTGCTTGCTTAAAAACCTCTTAATAAAGCTGCCAGTTAGAAGCAAGTTAAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 TGTGTGCTCCCATCTCTCCTAGTCGCCGCCTGGTCATTCGGTGTTCACCTGAGTAACAAG 
               
               
                          *       200         *         *         *         * 
               
               
                   
               
               
                 ACCCTGGTCTGTTAGGACCCTTCTTGCTTTGGGAAACCGAGGCAGGAAAATCCCTAGCAG 
               
               
                          *         *         *         *         *       300 
               
               
                   
               
               
                 GTTGGCGCCTGAACAGGGACTTGAAGAAGACTGAGAAGTCTTGGAACACGGCTGAGTGAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 GGCAGTAAGGGCGGCAGGAACAAACCACGACGGAGTGCTCCTAGAAAGGCGCGGGCCGAG 
               
               
                          *         *         *       400         *         * 
               
               
                   
               
               
                 GTACCAAAGGCAGCGTGTGGAGCGGGAGGAGAAGAGGCCTCCGGGTGAAGGTAAGTACCT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 ACACCAAAAACTGTAGCCGAAAGGGCTTGCTATCCTACCTTTAGACAGGTAGAAGATTGT 
               
               
                          *       500         *         *         *         * 
               
               
                   
               
               
                      MetGlyAlaArgAsnSerValLeuArgGlyLysLysAlaAspGluLeuGluArgIle 
               
               
                 GGGAGATGGGCGCGAGAAACTCCGTCTTGAGAGGGAAAAAAGCAGATGAATTAGAAAGAA 
               
               
                          *         *         *         *         *       600 
               
               
                   
               
               
                   ArgLeuArgProGlyGlyLysLysLysTyrArgLeuLysHisIleValTrpAlaAlaAsn 
               
               
                 TCAGGTTACGGCCCGGCGGAAAGAAAAAGTACAGGCTAAAACATATTGTGTGGGCAGCGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   LysLeuAspArgPheGlyLeuAlaGluSerLeuLeuGluSerLysGluGlyCysGlnLys 
               
               
                 ATAAATTGGACAGATTCGGATTAGCAGAGAGCCTGTTGGAGTCAAAAGAGGGTTGTCAAA 
               
               
                          *         *         *       700         *         * 
               
               
                   
               
               
                   IleLeuThrValLeuAspProMetValProThrGlySerGluAsnLeuLysSerLeuPhe 
               
               
                 AAATTCTTACAGTTTTAGATCCAATGGTACCGACAGGTTCAGAAAATTTAAAAAGTCTTT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   AsnThrValCysValIleTrpCysIleHisAlaGluGluLysValLysAspThrGluGly 
               
               
                 TTAATACTGTCTGCGTCATTTGGTGCATACACGCAGAAGAGAAAGTGAAAGATACTGAAG 
               
               
                          *       800         *         *         *         * 
               
               
                   
               
               
                   AlaLysGlnIleValArgArgHisLeuValAlaGluThrGlyThrAlaGluLysMetPro 
               
               
                 GAGCAAAACAAATAGTGCGGAGACATCTAGTGGCAGAAACAGGAACTGCAGAGAAAATGG 
               
               
                          *         *         *         *         *       900 
               
               
                   
               
               
                   SerThrSerArgProThrAlaProSerSerGluLysGlyGlyAsnTyrProValGlnHis 
               
               
                 CAAGCACAAGTAGACCAACAGCACCATCTAGCGAGAAGGGAGGAAATTACCCAGTGCAAC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ValGlyGlyAsnTyrThrHisIleProLeuSerProArgThrLeuAsnAlaTrpValLys 
               
               
                 ATGTAGGCGGCAACTACACCCATATACCGCTGAGTCCCCGAACCCTAAATGCCTGGGTAA 
               
               
                          *         *         *      1000         *         * 
               
               
                   
               
               
                   LeuValGluGluLysLysPheGlyAlaGluValValProGlyPheGlnAlaLeuSerGlu 
               
               
                 AATTAGTAGAGGAAAAAAAGTTCGGGGCAGAAGTAGTGCCAGGATTTCAGGCACTCTCAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   GlyCysThrProTyrAspIleAsnGlnMetLeuAsnCysValGlyAspHisGlnAlaAla 
               
               
                 AAGGCTGCACGCCCTATGATATCAACCAAATGCTTAATTGTGTGGGCGACCATCAAGCAG 
               
               
                          *      1100         *         *         *         * 
               
               
                   
               
               
                   MetGlnIleIleArgGluIleIleAsnGluGluAlaAlaGluTrpAspValGlnLisPro 
               
               
                 CCATGCAGATAATCAGGGAGATTATCAATGAGGAAGCAGCAGAATGGGATGTGCAACATC 
               
               
                          *         *         *         *         *      1200 
               
               
                   
               
               
                   IleProGlyProLeuProAlaGlyGlnLeuArgGluProArgGlySerAspIleAlaGly 
               
               
                 CAATACCAGGCCCCTTACCAGCGGGGCAGCTTAGAGAGCCAAGGGGATCTGACATAGCAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ThrThrSerThrValGluGluGlnIleGlnTrpMetPheArgProGlnAsnProValPro 
               
               
                 GGACAACAAGCACAGTAGAAGAACAGATCCAGTGGATGTTTAGGCCACAAAATCCTGTAC 
               
               
                          *         *         *      1300         *         * 
               
               
                   
               
               
                   ValGlyAsnIleTyrArgArgTrpIleGlnIleGlyLeuGlnLysCysValArgMetTyr 
               
               
                 CAGTAGGAAACATCTATAGAAGATGGATCCAGATAGGATTGCAGAAGTGTGTCAGGATGT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   AsnProThrAsnIleLeuAspIleLysGlnGlyProLysGluProPheGlnSerTyrVal 
               
               
                 ACAACCCGACCAACATCCTAGACATAAAACAGGGACCAAAGGAGCCGTTCCAAAGCTATG 
               
               
                          *      1400         *         *         *         * 
               
               
                   
               
               
                   AspArgPheTyrLysSerLeuArgAlaGluGlnThrAspProAlaValLysAsnTrpMet 
               
               
                 TAGATAGATTCTACAAAAGCTTGAGGGCAGAACAAACAGATCCAGCAGTGAAGAATTGGA 
               
               
                          *         *         *         *         *      1500 
               
               
                   
               
               
                   ThrGlnThrLeuLeuValGlnAsnAlaAsnProAspCysLysLeuValLeuLysGlyLeu 
               
               
                 TGACCCAAACACTGCTAGTACAAAATGCCAACCCAGACTGTAAATTAGTGCTAAAAGGAC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   GlyMetAsnProThrLeuGluGluMetLeuThrAlaCysGlnGlyValGlyGlyProGly 
               
               
                 TAGGGATGAACCCTACCTTAGAAGAGATGCTGACCGCCTGTCAGGGGGTAGGTGGGCCAG 
               
               
                          *         *         *      1600         *         * 
               
               
                   
               
               
                   GlnLysAlaArgLeuMetAlaGluAlaLeuLysGluValIleGlyProAlaProIlePro 
               
               
                 GCCAGAAAGCTAGATTAATGGCAGAGGCCCTGAAAGAGGTCATAGGACCTGCCCCTATCC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   PheAlaAlaAlaGlnGlnArgLysAlaPheLysCysTrpAsnCysGlyLysGluGlyHis 
               
               
                 CATTCGCAGCAGCCCAGCAGAGAAAGGCATTTAAATGCTGGAACTGTGGAAAGGAAGGGC 
               
               
                          *      1700         *         *         *         * 
               
               
                   
               
               
                   SerAlaArgGlnCysArgAlaProArgArgGlnGlyCysTrpLysCysGlyLysProGly 
               
               
                 ACTCGGCAAGACAATGCCGAGCACCTAGAAGGCAGGGCTGCTGGAAGTGTGGTAAGCCAG 
               
               
                          *         *         *         *         *      1800 
               
               
                   
               
               
                                             ThrGlyArgPhePheArgThrGlyProLeuGly 
               
               
                   HisIleMetThrAsnCysProAspArgGlnAlaGlyPheLeuGlyLeuGlyProTrpGly 
               
               
                 GACACATCATGACAAACTGCCCAGATAGACAGGCAGGTTTTTTAGGACTGGGCCCTTGGG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LysGluAlaProGlnLeuProArgGlyProSerSerAlaGlyAlaAspThrAsnSerThr 
               
               
                   LysLysProArgAsnPheProValAlaGlnValProGlnGlyLeuThrProThrAlaPro 
               
               
                 GAAAGAAGCCCCGCAACTTCCCCGTGGCCCAAGTTCCGCAGGGGCTGACACCAACAGCAC 
               
               
                          *         *         *      1900         *         * 
               
               
                   
               
               
                  ProSerGlySerSerSerGlySerThrGlyGluIleTyrAlaAlaArgGluLysThrGlu 
               
               
                   ProValAspProAlaValAspLeuLeuGluLysTyrMetGlnGlnGlyLysArgGlnArg 
               
               
                 CCCCAGTGGATCCAGCAGTGGATCTACTGGAGAAATATATGCAGCAAGGGAAAAGACAGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  ArgAlaGluArgGluThrIleGlnGlySerAspArgGlyLeuThrAlaProArgAlaGly 
               
               
                   GluGlnArgGluArgProTyrLysGluValThrGluAspLeuLeuHisLeuGluGlnGly 
               
               
                 GAGAGCAGAGAGAGAGACCATACAAGGAAGTGACAGAGGACTTACTGCACCTCGAGCAGG 
               
               
                          *      2000         *         *         *         * 
               
               
                   
               
               
                  GlyAspThrIleGlnGlyAlaThrAsnArgGlyLeuAlaAlaProGlnPheSerLeuTrp 
               
               
                   GluThrProTyrArgGluProProThrGluAspLeuLeuHisLeuAsnSerLeuPheGly 
               
               
                 GGGAGACACCATACAGGGAGCCACCAACAGAGGACTTGCTGCACCTCAATTCTCTCTTTG 
               
               
                          *         *         *         *         *      2100 
               
               
                   
               
               
                  LysArgProValValThrAlaTyrIleGluGlyGlnProValGluValLeuLeuAspThr 
               
               
                   LysAspGln 
               
               
                 GAAAAGACCAGTAGTCACAGCATACATTGAGGGTCAGCCAGTAGAAGTCTTGTTAGACAC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  GlyAlaAspAspSerIleValAlaGlyIleGluLeuGlyAsnAsnTyrSerProLysIle 
               
               
                 AGGGGCTGACGACTCAATAGTAGCAGGAATAGAGTTAGGGAACAATTATAGCCCAAAAAT 
               
               
                          *         *         *      2200         *         * 
               
               
                   
               
               
                  ValGlyGlyIleGlyGlyPheIleAsnThrLysGluTyrLysAsnValGluIleGluVal 
               
               
                 AGTAGGGGGAATAGGGGGATTCATAAATACCAAGGAATATAAAAATGTAGAAATAGAAGT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LeuAsnLysLysValArgAlaThrIleMetThrGlyAspThrProIleAsnIlePheGly 
               
               
                 TCTAAATAAAAAGGTACGGGCCACCATAATGACAGGCGACACCCCAATCAACATTTTTGG 
               
               
                          *      2300         *         *         *         * 
               
               
                   
               
               
                  ArgAsnIleLeuThrAlaLeuGlyMetSerLeuAsnLeuProValAlaLysValGluPro 
               
               
                 CAGAAATATTCTGACAGCCTTAGGCATGTCATTAAATCTACCAGTCGCCAAAGTAGAGCC 
               
               
                          *         *         *         *         *      2400 
               
               
                   
               
               
                  IleLysIleMetLeuLysProGlyLysAspGlyProLysLeuArgGlnTrpProLeuThr 
               
               
                 AATAAAAATAATGCTAAAGCCAGGGAAAGATGGACCAAAACTGAGACAATGGCCCTTAAC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LysGluLysIleGluAlaLeuLysGluIleCysGluLysMetGluLysGluGlyGlnLeu 
               
               
                 AAAAGAAAAAATAGAAGCACTAAAAGAAATCTGTGAAAAAATGGAAAAAGAAGGCCAGCT 
               
               
                          *         *         *      2500         *         * 
               
               
                   
               
               
                  GluGluAlaProProThrAsnProTyrAsnThrProThrPheAlaIleLysLysLysAsp 
               
               
                 AGAGGAAGCACCTCCAACTAATCCTTATAATACCCCCACATTTGCAATCAAGAAAAAGGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LysAsnLysTrpArgMetLeuIleAspPheArgGluLeuAsnLysValThrGlnAspPhe 
               
               
                 CAAAAACAAATGGAGGATGCTAATAGATTTCAGAGAACTAAACAAGGTAACTCAAGATTT 
               
               
                          *      2600         *         *         *         * 
               
               
                   
               
               
                  ThrGluIleGlnLeuGlyIleProHisProAlaGlyLeuAlaLysLysArgArgIleThr 
               
               
                 CACAGAAATTCAGTTAGGAATTCCACACCCAGCAGGGTTGGCCAAGAAGAGAAGAATTAC 
               
               
                          *         *         *         *         *      2700 
               
               
                   
               
               
                  ValLeuAspValGlyAspAlaTyrPheSerIleProLeuHisGluAspPheArgProTyr 
               
               
                 TGTACTAGATGTAGGGGATGCTTACTTTTCCATACCACTACATGAGGACTTTAGACCATA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  ThrAlaPheThrLeuProSerValAsnAsnAlaGluProGlyLysArgTyrIleTyrLys 
               
               
                 TACTGCATTTACTCTACCATCAGTGAACAATGCAGAACCAGGAAAAAGATACATATATAA 
               
               
                          *         *         *      2800         *         * 
               
               
                   
               
               
                  ValLeuProGlnGlyTrpLysGlySerProAlaIlePheGlnHisThrMetArgGlnVal 
               
               
                 AGTCTTGCCACAGGGATGGAAGGGATCACCAGCAATTTTTCAACACACAATGAGACAGGT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LeuGluProPheArgLysAlaAsnLysAspValIleIleIleGlnTyrMetAspAspIle 
               
               
                 ATTAGAACCATTCAGAAAAGCAAACAAGGATGTCATTATCATTCAGTACATGGATGATAT 
               
               
                          *      2900         *         *         *         * 
               
               
                   
               
               
                  LeuIleAlaSerAspArgThrAspLeuGluHisAspArgValValLeuGlnLeuLysGlu 
               
               
                 CTTAATAGCTAGTGACAGGACAGATTTAGAACATGATAGGGTAGTCCTGCAGCTCAAGGA 
               
               
                          *         *         *         *         *      3000 
               
               
                   
               
               
                  LeuLeuAsnGlyLeuGlyPheSerThrProAspGluLysPheGlnLysAspProProTyr 
               
               
                 ACTTCTAAATGGCCTAGGATTTTCTACCCCAGATGAGAAGTTCCAAAAAGACCCTCCATA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  HisTrpMetGlyTyrGluLeuTrpProThrLysTrpLysLeuGlnLysIleGlnLeuPro 
               
               
                 CCACTGGATGGGCTATGAACTATGGCCAACTAAATGGAAGTTGCAGAAAATACAGTTGCC 
               
               
                          *         *         *      3100         *         * 
               
               
                   
               
               
                  GlnLysGluIleTrpThrValAsnAspIleGlnLysLeuValGlyValLeuAsnTrpAla 
               
               
                 CCAAAAAGAAATATGGACAGTCAATGACATCCAGAAGCTAGTGGGTGTCCTAAATTGGGC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  AlaGlnLeuTyrProGlyIleLysThrLysHisLeuCysArgLeuIleArgGlyLysMet 
               
               
                 AGCACAACTCTACCCAGGGATAAAGACCAAACACTTATGTAGGTTAATCAGAGGAAAAAT 
               
               
                          *      3200         *         *         *         * 
               
               
                   
               
               
                  ThrLeuThrGluGluValGlnTrpThrGluLeuAlaGluAlaGluLeuGluGluAsnArg 
               
               
                 GACAGTCACAGAAGAAGTACAGTGGACAGAATTAGCAGAAGCAGAGCTAGAAGAAAACAG 
               
               
                          *         *         *         *         *      3300 
               
               
                   
               
               
                  IleIleLeuSerGlnGluGlnGluGlyHisTyrTyrGlnGluGluLysGluLeuGluAla 
               
               
                 AATTATCCTAAGCCAGGAACAAGAGGGACACTATTACCAAGAAGAAAAAGAGCTAGAAGC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  ThrValGlnLysAspGlnGluAsnGlnTrpThrTyrLysIleHisGlnGluGluLysIle 
               
               
                 AACAGTCCAAAAGGATCAAGAGAATCAGTGGACATATAAAATACACCAGGAAGAAAAAAT 
               
               
                          *         *         *      3400         *         * 
               
               
                   
               
               
                  LeuLysValGlyLysTyrAlaLysValLysAsnThrHisThrAsnGlyIleArgLeuLeu 
               
               
                 TCTAAAAGTAGGAAAATATGCAAAGGTGAAAAACACCCATACCAATGGAATCAGATTGTT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  AlaGlnValValGlnLysIleGlyLysGluAlaLeuValIleTrpGlyArgIleProLys 
               
               
                 AGCACAGGTAGTTCAGAAAATAGGAAAAGAAGCACTAGTCATTTGGGGACGAATACCAAA 
               
               
                          *      3500         *         *         *         * 
               
               
                   
               
               
                  PheHisLeuProValGluArgGluIleTrpGluGlnTrpTrpAspAsnTyrTrpGlnVal 
               
               
                 ATTTCACCTACCAGTAGAGAGAGAAATCTGGGAGCAGTGGTGGGATAACTACTGGCAAGT 
               
               
                          *         *         *         *         *      3600 
               
               
                   
               
               
                  ThrTrpIleProAspTrpAspPheValSerThrProProLeuValArgLeuAlaPheAsn 
               
               
                 GACATGGATCCCAGACTGGGACTTCGTGTCTACCCCACCACTGGTCAGGTTAGCGTTTAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LeuValGlyAspProIleProGlyAlaGluThrPheTyrThrAspGlySerCysAsnArg 
               
               
                 CCTGGTAGGGGATCCTATACCAGGTGCAGAGACCTTCTACACAGATGGATCCTGCAATAG 
               
               
                          *         *         *      3700         *         * 
               
               
                   
               
               
                   GlnSerLysGluGlyLysAlaGlyTyrValThrAspArgGlyLysAspLysValLysLys 
               
               
                 GCAATCAAAAGAAGGAAAAGCAGGATATGTAACAGATAGAGGGAAAGACAAGGTAAAGAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  LeuGluGlnThrThrAsnGlnGlnAlaGluLeuGluAlaPheAlaMetAlaLeuThrAsp 
               
               
                 ACTAGAGCAAACTACCAATCAGCAAGCAGAACTAGAAGCCTTTGCGATGGCACTAACAGA 
               
               
                          *      3800         *         *         *         * 
               
               
                   
               
               
                  SerGlyProLysValAsnIleIleValAspSerGlnTyrValMetGlyIleSerAlaSer 
               
               
                 CTCGGGTCCAAAAGTTAATATTATAGTAGACTCACAGTATGTAATGGGGATCAGTGCAAG 
               
               
                          *         *         *         *         *      3900 
               
               
                   
               
               
                  GlnProThrGluSerGluSerLysIleValAsnGlnIleIleGluGluMetIleLysLys 
               
               
                 CCAACCAACAGAGTCAGAAAGTAAAATAGTCAACCAGATCATAGAAGAAATGATAAAAAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  GluAlaIleTyrValAlaTrpValProAlaHisLysGlyIleGlyGlyAsnGlnGluVal 
               
               
                 GGAAGCAATCTATGTTGCATGGGTCCCAGCCCACAAAGGCATAGGGGGAAACCAGGAAGT 
               
               
                          *         *         *      4000         *         * 
               
               
                   
               
               
                  AspHisLeuValSerGlnGlyIleArgGlnValLeuPheLeuGluLysIleGluProAla 
               
               
                 AGATCATTTAGTGAGTCAGGGTATCAGACAAGTGTTGTTCCTGGAAAAAATAGAGCCCGC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  GlnGluGluHisGluLysTyrHisSerAsnValLysGluLeuSerHisLysPheGlyIle 
               
               
                 TCAGGAAGAACATGAAAAATATCATAGCAATGTAAAAGAACTGTCTCATAAATTTGGAAT 
               
               
                          *      4100         *         *         *         * 
               
               
                   
               
               
                  ProAsnLeuValAlaArgGlnIleValAsnSerCysAlaGlnCysGlnGlnLysGlyGlu 
               
               
                 ACCCAATTTAGTGGCAAGGCAAATAGTAAACTCATGTGCCCAATGTCAACAGAAAGGGGA 
               
               
                          *         *         *         *         *      4200 
               
               
                   
               
               
                  AlaIleHisGlyGlnValAsnAlaGluLeuGlyThrTrpGlnMetAspCysThrHisLeu 
               
               
                 AGCTATACATGGGCAAGTAAATGCAGAACTAGGCACTTGGCAAATGGACTGCACACATTT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  GluGlyLysIleIleIleValAlaValHisValAlaSerGlyPheIleGluAlaGluVal 
               
               
                 AGAAGGAAAGATCATTATAGTAGCAGTACATGTTGCAAGTGGATTTATAGAAGCAGAAGT 
               
               
                          *         *         *      4300         *         * 
               
               
                   
               
               
                  IleProGlnGluSerGlyArgGlnThrAlaLeuPheLeuLeuLysLeuAlaSerArgTrp 
               
               
                 CATCGCACAGGAATCAGGAAGACAAACAGCACTCTTCCTATTGAAACTGGCAAGTAGGTG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  ProIleThrHisLeuHisThrAspAsnGlyAlaAsnPheThrSerGlnGluValLysMet 
               
               
                 GCCAATAACACACTTGCATACAGATAATGGTGCCAACTTCACTTCACAGGAGGTGAAGAT 
               
               
                          *      4400         *         *         *         * 
               
               
                   
               
               
                  ValAlaTrpTrpIleGlyIleGluGlnSerPheGlyValProTyrAsnProGlnSerGln 
               
               
                 GGTAGCATGGTGGATAGGTATAGAACAATCCTTTGGAGTACCTTACAATCCACAGAGCCA 
               
               
                          *         *         *         *         *      4500 
               
               
                   
               
               
                  GlyValValGluAlaMetAsnHisHisLeuLysAsnGluIleSerArgIleArgGluGln 
               
               
                 AGGAGTAGTAGAAGCAATGAATCACCATCTAAAAAACCAAATAAGTAGAATCAGAGAACA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  AlaAsnThrIleGluThrIleValLeuMetAlaIleHisCysMetAsnPheLysArgArg 
               
               
                 GGCAAATACAATAGAAACAATAGTACTAATGGCAATTCATTGCATGAATTTTAAAAGAAG 
               
               
                          *         *         *      4600         *         * 
               
               
                   
               
               
                  GlyGlyIleGlyAspMetThrProSerGluArgLeuIleAsnMetIleThrThrGluGln 
               
               
                 GGGGGGAATAGGGGATATGACTCCATCAGAAAGATTAATCAATATGATCACCACAGAACA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  GluIleGlnPheLeuGlnAlaLysAsnSerLysLeuLysAspPheArgValTyrPheArg 
               
               
                 AGAGATACAATTCCTCCAAGCCAAAAATTCAAAATTAAAAGATTTTCGGGTCTATTTCAG 
               
               
                          *      4700         *         *         *         * 
               
               
                   
               
               
                  GluGlyArgAspGluLeuTrpLysGlyProGlyGluLeuLeuTrpLysGlyGluGlyAla 
               
               
                 AGAAGGCAGAGATCAGTTGTGGAAAGGACCTGGGGAACTACTGTGGAAAGGAGAAGGAGC 
               
               
                          *         *         *         *         *      4800 
               
               
                   
               
               
                  ValLeuValLysValGlyThrAspIleLysIleIleProArgArgLysAlaLysIleIle 
               
               
                 AGTCCTAGTCAAGGTAGGAACAGACATAAAAATAATACCAAGAAGGAAAGCCAAGATCAT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                  ArgAspTyrGlyGlyArgGlnGluMetAspSerGlySerHisLeuGluGlyAlaArgGlu 
               
               
                         MetGluGluAspLysArgTrpIleValValProThrTrpArgValProGlyArg 
               
               
                 CAGAGACTATGGAGGAAGACAAGAGATGGATAGTGGTTCCCACCTGGAGGGTGCCAGGGA 
               
               
                          *         *         *      4900         *         * 
               
               
                   
               
               
                  AspGlyGluMetAla 
               
               
                   MetGluLysTrpHisSerLeuValLysTyrLeuLysTyrLysThrLysAspLeuGluLys 
               
               
                 GGATGGAGAAATGGCATAGCCTTGTCAAGTATCTAAAATACAAAACAAAGGATCTAGAAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ValCysTyrValProHisHisLysValGlyTrpAlaTrpTrpThrCysSerArgValIle 
               
               
                 AGGTGTGCTATGTTCCCCACCATAAGGTGGGATGGGCATGGTGGACTTGCAGCAGGGTAA 
               
               
                            *      5000         *         *         *         * 
               
               
                   
               
               
                   PheProLeuLysGlyAsnSerHisLeuGluIleGlnAlaTyrTrpAsnLeuThrProGlu 
               
               
                 TATTCCCATTAAAAGGAAACAGTCATCTAGAGATACAGGCATATTGGAACTTAACACCAG 
               
               
                          *         *         *         *         *      5100 
               
               
                   
               
               
                   LysGlyTrpLeuSerSerTyrSerValArgIleThrTrpTyrThrGluLysPheTrpThr 
               
               
                 AAAAAGGATGGCTCTCCTCTTATTCAGTAAGAATAACTTGGTACACAGAAAAGTTCTGGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   AspValThrProAspCysAlaAspValLeuIleHisSerThrTyrPheProCysPheThr 
               
               
                 CAGATGTTACCCCAGACTGTGCAGATGTCCTAATACATAGCACTTATTTCCCTTGCTTTA 
               
               
                          *         *         *      5200         *         * 
               
               
                   
               
               
                   AlaGlyGluValArgArgAlaIleArgGlyGluLysLeuLeuSerCysCysAsnTyrPro 
               
               
                 CAGCAGGTGAAGTAAGAAGAGCCATCAGAGGGGAAAAGTTATTGTCCTGCTGCAATTATC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ArgAlaHisArgAlaGlnValProSerLeuGlnPheLeuAlaLeuValValValGlnGln 
               
               
                 CCCGAGCTCATAGAGCCCAGGTACCGTCACTTCAATTTCTGGCCTTAGTGGTAGTGCAAC 
               
               
                          *      5300         *         *         *         * 
               
               
                   
               
               
                    MetThrAspProArgGluThrValProProGlyAsnSerGlyGluGluThrIleGly 
               
               
                   AsnAspArgProGlnArgAspSerThrThrArgLysGlnArgArgArgAspTyrArgArg 
               
               
                 AAAATGACAGACCCCAGAGAGACAGTACCACCAGGAAACAGCGGCGAAGAGACTATCGGA 
               
               
                          *         *         *         *         *      5400 
               
               
                   
               
               
                 GluAlaPheAlaTrpLeuAsnArgThrValGluAlaIleAsnArgGluAlaValAsnHis 
               
               
                   GlyLeuArgLeuAlaLysGlnAspSerArgSerHisLysGlnArgSerSerGluSerPro 
               
               
                 GAGGCCTTCGCCTGGCTAAACAGGACAGTAGAAGCCATAAACAGAGAAGCAGTGAATCAC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 LeuProArgGluLeuIlePheGlnValTrpGlnArgSerTrpArgTyrTrpHisAspGlu 
               
               
                   ThrProArgThrTyrPheProGlyValAlaGluValLeuGluIleLeuAla 
               
               
                 CTACCCCGAGAACTTATTTTCCAGGTGTGGCAGAGGTCCTGGAGATACTGGCATGATGAA 
               
               
                          *         *         *      5500         *         * 
               
               
                   
               
               
                 GlnGlyMetSerGluSerTyrThrLysTyrArgTyrLeuCysIleIleGlnLysAlaVal 
               
               
                 CAAGGGATGTCAGAAAGTTACACAAAGTATAGATATTTGTGCATAATACAGAAAGCAGTG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 TyrMetHisValArgLysGlyCysThrCysLeuGlyArgGlyHisGlyProGlyGlyTrp 
               
               
                 TACATGCATGTTAGGAAAGGGTGTACTTGCCTGGGGAGGGGACATGGGCCAGGAGGGTGG 
               
               
                          *      5600         *         *         *         * 
               
               
                   
               
               
                 ArgProGlyProProProProProProProGlyLeuVal 
               
               
                                                          MetAlaGluAlaProThrGlu 
               
               
                 AGACCAGGGCCTCCTCCTCCTCCCCCTCCAGGTCTGGTCTAATGGCTGAAGCACCAACAG 
               
               
                          *         *         *         *         *      5700 
               
               
                   
               
               
                   LeuProProValAspGlyThrProLeuArgGluProGlyAspGluTrpIleIleGluIle 
               
               
                 AGCTCCCCCCGGTGGATGGGACCCCACTGAGGGAGCCAGGGGATGAGTGGATAATAGAAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   LeuArgGluIleLysGluGluAlaLeuLysHisPheAspProArgLeuLeuIleAlaLeu 
               
               
                 TCTTGAGAGAAATAAAAGAAGAAGCTTTAAAGCATTTTGACCCTCGCTTGCTAATTGCTC 
               
               
                          *         *         *      5800         *         * 
               
               
                   
               
               
                                         MetGluThrPreLeuLysAlaProGluSerSerLeu 
               
               
                   GlyLysTyrIleTyrThrArgHisGlyAspThrLeuGluGlyAlaArgGluLeuIleLys 
               
               
                 TTGGCAAATATATCTATACTAGACATGGAGACACCCTTGAAGGCGCCAGAGAGCTCATTA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 LysSerCysAsnGluProPheSerArgThrSerGluGlnAspValAlaThrGlnGluLeu 
               
               
                   ValLeuGlnArgAlaLeuPheThrHisPheArgAlaGlyCysGlyHisSerArgIleGly 
               
               
                 AAGTCCTGCAACGAGCCCTTTTCACGCACTTCAGAGCAGGATGTGGCCACTCAAGAATTG 
               
               
                          *      5900         *         *         *         * 
               
               
                   
               
               
                 AlaArgGlnGlyGluGluIleLeuSerGlnLeuTyrArgProLeuGluThrCysAsnAsn 
               
               
                   GlnThrArgGlyGlyAsnProLeuSerAlaIleProThrProArgAsnMetGln 
               
               
                 GCCAGACAAGGGGAGGAAATCCTCTCTCAGCTATACCGACCCCTAGAAACATGCAATAAC 
               
               
                          *         *         *         *         *      6000 
               
               
                   
               
               
                 SerCysTyrCysLysArgCysCysTyrHisCysGlnMetCysPheLeuAsnLysGlyLeu 
               
               
                 TCATGCTATTGTAAGCGATGCTGCTACCATTGTCAGATGTGTTTTCTAAACAAGGGGCTC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 GlyIleCysTyrGluArgLysGlyArgArgArgArgThrProLysLysThrLysThrHis 
               
               
                           MetAsnGluArgAlaAspGluGluGlyLeuGlnArgLysLeuArgLeuIle 
               
               
                 GGGATATGTTATGAACGAAAGGGCAGACGAAGAAGGACTCCAAAGAAAACTAAGACTCAT 
               
               
                          *         *         *      6100         *         * 
               
               
                   
               
               
                 ProSerProThrProAspLys 
               
               
                  ArgLeuLeuHisGlnThr 
               
               
                                           MetMetAsnGlnLeuLeuIleAlaIleLeuLeuAla 
               
               
                 CCGTCTCCTACACCAGACAAGTGAGTATGATGAATCAGCTGCTTATTGCCATTTTATTAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   SerAlaCysLeuValTyrCysThrGlnTyrValThrValPheTyrGlyValProThrTrp 
               
               
                 CTAGTGCTTGCTTAGTATATTGCACCCAATATGTAACTGTTTTCTATGGCGTACCCACGT 
               
               
                          *      6200         *         *         *         * 
               
               
                   
               
               
                   LysAsnAlaThrIleProLeuPheCysAlaThrArgAsnArgAspThrTrpGlyThrlle 
               
               
                 GGAAAAATGCAACCATTCCCCTCTTTTGTGCAACCAGAAATAGGGATACTTGGGGAACCA 
               
               
                          *         *         *         *         *      6300 
               
               
                   
               
               
                   GlnCysLeuProAspAsnAspAspTyrGlnGluIleThrLeuAsnValThrGluAlaPhe 
               
               
                 TACAGTGCTTGCCTGACAATGATGATTATCAGGAAATAACTTTGAATGTAACAGAGGCTT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   AspAlaTrpAsnAsnThrValThrGluGlnAlaIleGluAspValTrpHisLeuPheGlu 
               
               
                 TTGATGCATGGAATAATACAGTAACAGAACAAGCAATAGAAGATGTCTGGCATCTATTCG 
               
               
                          *         *         *      6400         *         * 
               
               
                   
               
               
                   ThrSerIleLysProCysValLysLeuThrProLeuCysValAlaMetLysCysSerSer 
               
               
                 AGACATCAATAAAACCATGTGTCAAACTAACACCTTTATGTGTAGCAATGAAATGCAGCA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ThrGluSerSerThrGlyAsnAsnThrThrSerLysSerThrSerThrThrThrThrThr 
               
               
                 GCACAGAGAGCAGCACAGGGAACAACACAACCTCAAAGAGCACAAGCACAACCACAACCA 
               
               
                          *      6500         *         *         *         * 
               
               
                   
               
               
                   ProThrAspGlnGluGlnGluIleSerGluAspThrProCysAlaArgAlaAspAsnCys 
               
               
                 CACCCACAGACCAGGAGCAAGAGATAAGTGAGGATACTCCATGCGCACGCGCAGACAACT 
               
               
                          *         *         *         *         *      6600 
               
               
                   
               
               
                   SerGlyLeuGlyGluGluGluThrIleAsnCysGlnPheAsnMetThrGlyLeuGluArg 
               
               
                 GCTCAGGATTGGGAGAGGAAGAAACGATCAATTGCCAGTTCAATATGACAGGATTAGAAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   AspLysLysLysGlnTyrAsnGluThrTrpTyrSerLysAspValValCysGluThrAsn 
               
               
                 GAGATAAGAAAAAACAGTATAATGAAACATGGTACTCAAAAGATGTGGTTTGTGAGACAA 
               
               
                          *         *         *      6700         *         * 
               
               
                   
               
               
                   AsnSerThrAsnGlnThrGlnCysTyrMetAsnHisCysAsnThrSerValIleThrGlu 
               
               
                 ATAATAGCACAAATCAGACCCAGTGTTACATGAACCATTGCAACACATCAGTCATCACAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   SerCysAspLysHisTyrTrpAspAlaIleArgPheArgTyrCysAlaProProGlyTyr 
               
               
                 AATCATGTGACAAGCACTATTGGGATGCTATAAGGTTTAGATACTGTGCACCACCGGGTT 
               
               
                          *      6800         *         *         *         * 
               
               
                   
               
               
                   AlaLeuLeuArgCysAsnAspThrAsnTyrSerGlyPheAlaProAsnCysSerLysVal 
               
               
                 ATGCCCTATTAAGATGTAATGATACCAATTATTCAGGCTTTGCACCCAACTGTTCTAAAG 
               
               
                          *         *         *         *         *      6900 
               
               
                   
               
               
                   ValAlaSerThrCysThrArgMetMetGluThrGlnThrSerThrTrpPheGlyPheAsn 
               
               
                 TAGTAGCTTCTACATGCACCAGGATGATGGAAACGCAAACTTCCACATGGTTTGGCTTTA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   GlyThrArgAlaGluAsnArgThrTyrIleTyrTrpHisGlyArgAspAsnArgThrIle 
               
               
                 ATGGCACTAGAGCAGAGAATAGAACATATATCTATTGGCATGGCAGAGATAATAGAACTA 
               
               
                          *         *         *      7000         *         * 
               
               
                   
               
               
                   IleSerLeuAsnLysTyrTyrAsnLeuSerLeuHisCysLysArgProGlyAsnLysThr 
               
               
                 TCATCAGCTTAAACAAATATTATAATCTCAGTTTGCATTGTAAGAGGCCAGGGAATAAGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ValLysGlnIleMetLeuMetSerGlyHisValPheHisSerHisTyrGlnProIleAsn 
               
               
                 CAGTGAAACAAATAATGCTTATGTCAGGACATGTGTTTCACTCCCACTACCAGCCGATCA 
               
               
                          *      7100         *         *         *         * 
               
               
                   
               
               
                   LysArgProArgGlnAlaTrpCysTrpPheLysGlyLysTrpLysAspAlaMetGlnGlu 
               
               
                 ATAAAAGACCCAGACAAGCATGGTGCTGGTTCAAAGGCAAATGGAAAGACGCCATGCAGG 
               
               
                          *         *         *         *         *      7200 
               
               
                   
               
               
                   ValLysGluThrLeuAlaLysHisProArgTyrArgGlyThrAsnAspThrArgAsnIle 
               
               
                 AGGTGAAGGAAACCCTTGCAAAACATCCCAGGTATAGAGGAACCAATGACACAAGGAATA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   SerPheAlaAlaProGlyLysGlySerAspProGluValAlaTyrMetTrpThrAsnCys 
               
               
                 TTAGCTTTGCAGCGCCAGGAAAAGGCTCAGACCCAGAAGTAGCATACATGTGGACTAACT 
               
               
                          *         *         *      7300         *         * 
               
               
                   
               
               
                   ArgGlyGluPheLeuTyrCysAsnMetThrTrpPheLeuAsnTrpIleGluAsnLysThr 
               
               
                 GCAGAGGAGAGTTTCTCTACTGCAACATGACTTGGTTCCTCAATTGGATAGAGAATAAGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   HisArgAsnTyrAlaProCysHisIleLysGlnIleIleAsnThrTrpHisLysValGly 
               
               
                 CACACCGCAATTATGCACCGTGCCATATAAAGCAAATAATTAACACATGGCATAAGGTAG 
               
               
                          *      7400         *         *         *         * 
               
               
                   
               
               
                   ArgAsnValTyrLeuProProArgGluGlyGluLeuSerCysAsnSerThrValThrSer 
               
               
                 GGAGAAATGTATATTTGCCTCCCAGGGAAGGGGAGCTGTCCTGCAACTCAACAGTAACCA 
               
               
                          *         *         *         *         *      7500 
               
               
                   
               
               
                   IleIleAlaAsnIleAspTrpGlnAsnAsnAsnGlnThrAsnIleThrPheSerAlaGlu 
               
               
                 GCATAATTGCTAACATTGACTGGCAAAACAATAATCAGACAAACATTACCTTTAGTGCAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ValAlaGluLeuTyrArgLeuGluLeuGlyAspTyrLysLeuValGluIleThrProIle 
               
               
                 AGGTGGCAGAACTATACAGATTGGAGTTGGGAGATTATAAATTGGTAGAAATAACACCAA 
               
               
                          *         *         *      7600         *         * 
               
               
                   
               
               
                   GlyPheAlaProThrLysGluLysArgTyrSerSerAlaHisGlyArgHisThrArgGly 
               
               
                 TTGGCTTCGCACCTACAAAAGAAAAAAGATACTCCTCTGCTCACGGGAGACATACAAGAG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   ValPheValLeuGlyPheLeuGlyPheLeuAlaThrAlaGlySerAlaMetGlyAlaAla 
               
               
                 GTGTGTTCGTGCTAGGGTTCTTGGGTTTTCTCGCAACAGCAGGTTCTGCAATGGGCGCGG 
               
               
                          *      7700         *         *         *         * 
               
               
                   
               
               
                   SerLeuThrValSerAlaGlnSerArgThrLeuLeuAlaGlyIleValGlnGlnGlnGln 
               
               
                 CGTCCCTGACCGTGTCGGCTCAGTCCCGGACTTTACTGGCCGGGATAGTGCAGCAACAGC 
               
               
                          *         *         *        *         *      7800 
               
               
                   
               
               
                   GlnLeuLeuAspValValLysArgGlnGlnGluLeuLeuArgLeuThrValTrpGlyThr 
               
               
                 AACAGCTGTTGGACGTGGTCAAGAGACAACAAGAACTGTTGCGACTGACCGTCTGGGGAA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   LysAsnLeuGlnAlaArgValThrAlaIleGluLysTyrLeuGlnAspGlnAlaArgLeu 
               
               
                 CGAAAAACCTCCAGGCAAGAGTCACTGCTATAGAGAAGTACCTACAGGACCAGGCGCGGC 
               
               
                          *         *         *      7900         *         * 
               
               
                   
               
               
                   AsnSerTrpGlyCysAlaPheArgGlnValCysHisThrThrValProTrpValAsnAsp 
               
               
                 TAAATTCATGGGGATGTGCGTTTAGACAAGTCTGCCACACTACTGTACCATGGGTTAATG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   SerLeuAlaProAspTrpAspAsnMetThrTrpGlnGluTrpGluLysGlnValArgTyr 
               
               
                 ATTCCTTAGCACCTGACTGGGACAATATGACGTGGCAGGAATGGGAAAAACAAGTCCGCT 
               
               
                          *      8000         *         *         *         * 
               
               
                   
               
               
                   LeuGluAlaAsnIleSerLysSerLeuGluGlnAlaGlnIleGlnGlnGluLysAsnMet 
               
               
                 ACCTGGAGGCAAATATCAGTAAAAGTTTAGAACAGGCACAAATTCAGCAAGAGAAAAATA 
               
               
                          *         *         *         *         *      8100 
               
               
                   
               
               
                   TyrGluLeuGlnLysLeuAsnSerTrpAspIlePheGlyAsnTrpPheAspLeuThrSer 
               
               
                 TGTATGAACTACAAAAATTAAATAGCTGGGATATTTTTGGCAATTGGTTTGACTTAACCT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                   TrpValLysTyrIleGlnTyrGlyValLeuIleIleValAlaValIleAlaLeuArgIle 
               
               
                 CCTGGGTCAAGTATATTCAATATGGAGTGCTTATAATAGTAGCAGTAATAGCTTTAAGAA 
               
               
                          *         *         *      8200         *         * 
               
               
                   
               
               
                   ValIleTyrValValGlnMetLeuSerArgLeuArgLysGlyTyrArgProValPheSer 
               
               
                 TAGTGATATATGTAGTACAAATGTTAAGTAGGCTTAGAAAGGGCTATAGGCCTGTTTTCT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                                            SerIleSerThrArgThrGlyAspSerGlnPro 
               
               
                                          AsnProTyrProGlnGlyProGlyThrAlaSerGln 
               
               
                   SerProProGlyTyrIleGlnGlnIleHisIleHisLysAspArgGlyGlnProAlaAsn 
               
               
                 CTTCCCCCCCCGGTTATATCCAACAGATCCATATCCACAAGGACCGGGGACAGCCAGCCA 
               
               
                          *      8300         *         *         *         * 
               
               
                   
               
               
                 ThrLysLysGlnLysLysThrValGluAlaThrValGluThrAspThrGlyProGlyArg 
               
               
                  ArgArgAsnArgArgArgArgTrpLysGlnArgTrpArgGlnIleLeuAlaLeuAlaAsp 
               
               
                   GluGluThrGluGluAspGlyGlySerAsnGlyGlyAspArgTyrTrpProTrpProIle 
               
               
                 ACGAAGAAACAGAAGAAGACGGTGGAAGCAACGGTGGAGACAGATACTGGCCCTGGCCGA 
               
               
                          *         *         *         *         *      8400 
               
               
                   
               
               
                  SerIleTyrThrPheProAspProProAlaAspSerProLeuAspGlnThrIleGlnHis 
               
               
                   AlaTyrIleHisPheLeuIleArgGlnLeuIleArgLeuLeuThrArgLeuTyrSerIle 
               
               
                 TAGCATATATACATTTCCTGATCCGCCAGCTGATTCGCCTCTTGACCAGACTATACAGCA 
               
               
                          *         *         *         *         *          * 
               
               
                   
               
               
                  LeuGlnGlyLeuThrIleGlnGluLeuProAspProProThrHisLeuProGluSerGln 
               
               
                   CysArgAspLeuLeuSerArgSerPheLeuThrLeuGlnLeuIleTyrGlnAsnLeuArg 
               
               
                 TCTGCAGGGACTTACTATCCAGGAGCTTCCTGACCCTCCAACTCATCTACCAGAATCTCA 
               
               
                          *         *         *      8500         *         * 
               
               
                   
               
               
                  ArgLeuAlAGluThr                    MetGlyAlaSerGlySerLysLys 
               
               
                   AspTrpLeuArgLeuArgThrAlaPheLeuGlnTyrGlyCysGluTrpIleGlnGluAla 
               
               
                 GAGACTGGCTGAGACTTAGAACAGCCTTCTTGCAATATGGGTGCGAGTGGATCCAAGAAG 
               
               
                          *         *          *         *         *         * 
               
               
                   
               
               
                 HisSerArgProProArgGlyLeuGlnGluArgLeuLeuArgAlaArgAlaGlyAlaCys 
               
               
                   PheGlnAlaAlaAlaArgAlaThrArgGluThrLeuAlaGlyAlaCysArgGlyLeuTrp 
               
               
                 CATTCCAGGCCGCCGCGAGGGCTACAAGAGAGACTCTTGCGGGCGCGTGCAGGGGCTTGT 
               
               
                        *      8600         *         *         *         * 
               
               
                   
               
               
                 GlyGlyTyrTrpAsnGluSerGlyGlyGluTyrSerArgPheGlnGluGlySerAspArg 
               
               
                   ArgValLeuGluArgIleGlyArgGlyIleLeuAlaValProArgArgIleArgGlnGly 
               
               
                 GGAGGGTATTGGAACGAATCGGGAGGGGAATACTCGCGGTTCCAAGAAGGATCAGACAGG 
               
               
                          *         *         *         *         *      8700 
               
               
                   
               
               
                 GluGlnLysSerProSerCysGluGlyArgGlnTyrGlnGlnGlyAspPheMetAsnThr 
               
               
                   AlaGluIleAlaLeuLeu 
               
               
                 GAGCAGAAATCGCCCTCCTGTGAGGGACGGCAGTATCAGCAGGGAGACTTTATGAATACT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 ProTrpLysAspProAlaAlaGluArgGluLysAsnLeuTyrArgGlnGlnAsnMetAsp 
               
               
                 CCATGGAAGGACCCAGCAGCAGAAAGGGAGAAAAATTTGTACAGGCAACAAAATATGGAT 
               
               
                          *         *         *      8800         *         * 
               
               
                   
               
               
                 AspValAspSerAspAspAspAspGlnValArgValSerValThrProLysValProLeu 
               
               
                 GATGTAGATTCAGATGATGATGACCAAGTAAGAGTTTCTGTCACACCAAAAGTACCACTA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 ArgProMetThrHisArgLeuAlaIleAspMetSerHisLeuIleLysThrArgGlyGly 
               
               
                 AGACCAATGACACATAGATTGGCAATAGATATGTCACATTTAATAAAAACAAGGGGGGGA 
               
               
                          *      8900         *         *         *         * 
               
               
                   
               
               
                 LeuGluGlyMetPheTyrSerGluArgArgHisLysIleLeuAsnIleTyrLeuGluLys 
               
               
                 CTGGAAGGGATGTTTTACAGTGAAAGAAGACATAAAATCTTAAATATATACTTAGAAAAG 
               
               
                          *         *         *         *         *      9000 
               
               
                   
               
               
                 GluGluGlyIleIleAlaAspTrpGlnAsnTyrThrHisGlyProGlyValArgTyrPro 
               
               
                 GAAGAAGGGATAATTGCAGATTGGCAGAACTACACTCATGGGCCAGGAGTAAGATACCCA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 MetPhePheGlyTrpLeuTrpLysLeuValProValAspValProGlnGluGlyGluAsp 
               
               
                 ATGTTCTTTGGGTGGCTATGGAAGCTAGTACCAGTAGATGTCCCACAAGAAGGGGAGGAC 
               
               
                          *         *         *      9100         *         * 
               
               
                   
               
               
                 ThrGluThrHisCysLeuValHisProAlaGlnThrSerLysPheAspAspProHisGly 
               
               
                 ACTGAGACTCACTGCTTAGTACATCCAGCACAAACAAGCAAGTTTGATGACCCGCATGGG 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 GluThrLeuValTrpGluPheAspProLeuLeuAlaTyrSerTyrGluAlaPheIleArg 
               
               
                 GAGACACTAGTCTGGGAGTTTGATCCCTTGCTGGCTTATAGTTACGAGGCTTTTATTCGG 
               
               
                          *      9200         *         *         *         * 
               
               
                   
               
               
                 TyrProGluGluPheGlyHisLysSerGlyLeuProGluGluGluTrpLysAlaArgLeu 
               
               
                 TACCCAGAGGAATTTGGGCACAAGTCAGGCCTGCCAGAGGAAGAGTGGAAGGCGAGACTG 
               
               
                          *         *         *         *         *      9300 
               
               
                   
               
               
                 LysAlaArgGlyIleProPheSer 
               
               
                 AAAGCAAGAGGAATACCATTTAGTTAAAGACAGGAACAGCTATACTTGGTCAGGGCAGGA 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 AGTAACTAACAGAAACAGCTGAGACTGCAGGGACTTTCCAGAAGGGGCTGTAACCAAGGG 
               
               
                          *         *         *      9400         *         * 
               
               
                   
               
               
                 AGGGACATGGGAGGAGCTGGTGGGGAACGCCCTCATATTCTCTGTATAAATATACCCGCT 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 AGCTTGCATTGTACTTCGGTCGCTCTGCGGAGAGGCTGGCAGATTGAGCCCTGGGAGGTT 
               
               
                          *      9500         *         *         *         * 
               
               
                   
               
               
                 CTCTCCAGCAGTAGCAGGTAGAGCCTGGGTGTTCCCTGCTAGACTCTCACCAGCACTTGG 
               
               
                          *         *         *         *         *      9600 
               
               
                   
               
               
                 CCGGTGCTGGGCAGACGGCCCCACGCTTGCTTGCTTAAAAACCTCCTTAATAAAGCTGCC 
               
               
                          *         *         *         *         *         * 
               
               
                   
               
               
                 AGTTAGAAGCA 
               
               
                          * 
               
             
          
         
       
     
     Example 5 
     Sequences of the Coding Regions for the Envelope Protein and GAG Product of the ROD HIV-2 Isolate 
     Through experimental analysis of the HIV-2 ROD isolate, the following sequences were identified for the regions encoding the env and gag gene products. One of ordinary skill in the art will recognize that the numbering for both gene regions which follow begins for convenience with “1” rather than the corresponding number for its initial nucleotide as given in Example 4, above, in the context of the complete genomic sequence. 
     
       
         
               
               
             
           
               
                   
                 MetMetAsnGluLeuLeuIleAlaIleLeuLeuAlaSerAlaCys 
               
               
                   
                 ATGATGAATCAGCTGCTTATTGCCATTTTATTAGCTAGTGCTTGC 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LeuValTyrCysThrGlnTyrValThrValPheTyrGlyValPro 
               
               
                   
                 TTAGTATATTGCACCCAATATGTAACTGTTTTCTATGGCGTACCC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ThrTrpLysAsnAlaThrIleProLeuPheCysAlaThrArgAsa 
               
               
                   
                 ACGTGGAAAAATGCAACCATTCCCCTGTTTTGTGCAACCAGAAAT 
               
               
                   
                   
               
               
                   
                        100         *         *         * 
               
               
                   
                 ArgAspThrTrpGlyThrIleGlaCysLeuProAspAsaAspAsp 
               
               
                   
                 AGGGATACTTGGGGAACCATACAGTGCTTGCCTGACAATGATGAT 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 TyrGluGluIleThrLeuAsnValThrGluAlaPheAspAlaTrp 
               
               
                   
                 TATCAGGAAATAACTTTGAATGTAACAGAGGCTTTTGATGCATGG 
               
               
                   
                   
               
               
                   
                          *       200         *         * 
               
               
                   
                 AsnAsaThrValThrGluGlnAlaIleGluAspValTrpHisLeu 
               
               
                   
                 AATAATACAGTAACAGAACAAGCAATAGAAGATGTCTGGCATCTA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 PheGluThrSerIleLysProCysValLysLeuThrProLeuCys 
               
               
                   
                 TTCGAGACATCAATAAAACCATGTGTGAAACTAACACCTTTATGT 
               
               
                   
                   
               
               
                   
                          *         *       300         * 
               
               
                   
                 ValAlaMetLysCysSerSerThrGluSerSerThrGlyAsnAsn 
               
               
                   
                 GTAGCAATGAAATGCAGCAGCACAGAGAGCAGCACAGGGAACAAC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ThrThrSerLysSerThrSerThrThrThrThrThrProThrAsp 
               
               
                   
                 ACAACCTCAAAGAGCACAAGCACAACCACAACCACACCCAGAGAC 
               
               
                   
                   
               
               
                   
                          *         *         *       400 
               
               
                   
                 GluGluGlnGluIleSerGluAspThrProCysAlaArgAlaAsp 
               
               
                   
                 CAGGAGCAAGAGATAAGTGAGGATACTCCATGCGCACGCGCACAC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 AsnCysSerGlyLeuGlyGluGluGluThrIleAsnCysGlnPhe 
               
               
                   
                 AACTGCTCAGGATTGGGAGAGGAAGAAACGATCAATTGCCAGTTC 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AsnMetThrGlyLeuGluArgAspLysLysLysGlnTyrAsnGlu 
               
               
                   
                 AATATGACAGGATTAGAAAGAGATAAGAAAAAACAGTATAATGAA 
               
               
                   
                   
               
               
                   
                   500         *         *         *         * 
               
               
                   
                 ThrTrpTyrSerLysAspValValCysGluThrAsnAsnSerThr 
               
               
                   
                 ACATGGTACTCAAAAGATGTGGTTTGTGAGACAAATAATAGCACA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AsnGlnThrGlnCysTyrMetAsnHisCysAsnThrSerValIle 
               
               
                   
                 AATCAGACCCAGTGTTACATGAACCATTGCAACACATCAGTCATC 
               
               
                   
                   
               
               
                   
                     *       600         *         *         * 
               
               
                   
                 ThrGluSerCysAspLysHisTyrTrpAspAlaIleArgPheArg 
               
               
                   
                 ACAGAATCATGTGACAAGCACTATTGGGATGCTATAAGGTTTAGA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 TyrCysAlaProProGlyTyrAlaLeuLeuArgCyaAsnAspThr 
               
               
                   
                 TACTGTGCACCACCGGGTTATGCCCTATTAAGATGTAATGATACC 
               
               
                   
                   
               
               
                   
                     *         *       700         *         * 
               
               
                   
                 AsnTyrSerGlyPheAlaProAsnCysSerLysValValAlaSer 
               
               
                   
                 AATTATTCAGGCTTTGCACCCAACTGTTCTAAAGTAGTAGCTTCT 
               
               
                   
                   
               
               
                   
                 ThrCysThrArgMetMetGluThrGluThrSerThrTrpPheGly 
               
               
                   
                 ACATGCACCAGGATGATGGAAACGCAAACTTCCACATGGTTTGGC 
               
               
                   
                   
               
               
                   
                     *         *         *       800         * 
               
               
                   
                 PheAsnGlyThrArgAlaGluAsnArgThrTyrIleTyrTrpHis 
               
               
                   
                 TTTAATGGCACTAGAGCAGAGAATAGAACATATATCTATTGGCAT 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 GlyArgAspAsnArgThrIleIleSerLeuAsnLysTyrTyrAsn 
               
               
                   
                 GGCAGAGATAATAGAACTATCATCAGCTTAAACAAATATTATAAT 
               
               
                   
                   
               
               
                   
                     *         *         *         *       900 
               
               
                   
                 LeuSerLeuHisCysLysArgProGlyAsnLysThrValLysGla 
               
               
                   
                 CTCAGTTTGCATTGTAAGAGGCCAGGGAATAAGACAGTGAAACAA 
               
               
                   
                   
               
               
                   
                          *         *         *         *         * 
               
               
                   
                 IleMetLeuMetSerGlyHisValPheHisSerHisTyrGlnPro 
               
               
                   
                 ATAATGCTTATGTCAGGACATGTGTTTCACTCCCACTACCAGCCG 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 IleAsnLysArgProArgGluAlaTrpCysTrpPheLysGlyLys 
               
               
                   
                 ATCAATAAAAGACCCAGACAAGCATGGTGCTGGTTCAAAGGCAAA 
               
               
                   
                   
               
               
                   
                       1000         *         *         * 
               
               
                   
                 TrpLysAspAlaMetGluGluValLysThrLeuAlaLysHisPro 
               
               
                   
                 TGGAAAGACGCCATGCAGGAGGTGAAGAGCCTTGCAAAACATCCC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ArgTyrArgGlyThrAsnAspThrArgAsnIleSerPheAlaAla 
               
               
                   
                 AGGTATAGAGGAACCAATGACACAAGGAATATTAGCTTTGCAGCG 
               
               
                   
                   
               
               
                   
                          *      1100         *         * 
               
               
                   
                 ProGlyLysGlySerAspProGluValAlaTyrMetTrpThrAsn 
               
               
                   
                 CCAGGAAAAGGCTCAGACCCACAAGTAGCATACATGTGGACTAAG 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 CysArgGlyGluPheLeuTyrCysAsnMetThrTrpPheLeuAsn 
               
               
                   
                 TGCAGAGGAGAGTTTCTCTACTGCAACATGACTTGGTTCCTCAAT 
               
               
                   
                   
               
               
                   
                          *         *      1200         * 
               
               
                   
                 TrpIleGluAsnLysThrHisArgAsnTyrAlaProCysHisIle 
               
               
                   
                 TGGATAGAGAATAAGACACACCGCAATTATGCACCGTGCCATATA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 LysGluIleIleAsnThrTrpHisLysValGlyArgAsnValTyr 
               
               
                   
                 AAGCAAATAATTAACACATGGCATAAGGTAGGGAGAAATGTATAT 
               
               
                   
                   
               
               
                   
                          *         *         *      1300 
               
               
                   
                 LeuProProArgGluGlyGluLeuSerCysAsnSerThrValThr 
               
               
                   
                 TTGCCTCCCAGGGAAGGGGAGCTGTCCTGGAACTCAACAGTAACC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 SerIleIleAlaAsnIleAspTrpGlnAsnAsnAsnGlnThrAsn 
               
               
                   
                 AGCATAATTGCTAACATTGACTGGCAAAACAATAATCAGACAAAC 
               
               
                   
                   
               
               
                   
                          *         *        *         * 
               
               
                   
                 IleThrPheSerAlaGluValAlaGluLeuTyrArgLeuGluLeu 
               
               
                   
                 ATTACCTTTAGTGCAGAGGTGGCAGAACTATACAGATTGGAGTTG 
               
               
                   
                   
               
               
                   
                  1400         *        *         *         * 
               
               
                   
                 GlyAspTyrLysLeuValGluIleThrProIleGlyPheAlaPro 
               
               
                   
                 GGAGATTATAAATTGGTAGAAATAACACCAATTGGCTTCGCACCT 
               
               
                   
                   
               
               
                   
                 ThrLysGluLysArgTyrSerSerAlaHisGlyArgHisThrArg 
               
               
                   
                 ACAAAAGAAAAAAGATACTCCTCTGCTCACGGGAGACATAGAAGA 
               
               
                   
                   
               
               
                   
                     *      1500         *         *         * 
               
               
                   
                 GlyValPheValLeuGlyPheLeuGlyPheLeuAlaThrAlaGly 
               
               
                   
                 GGTGTGTTCGTGCTAGGGTTCTTGGGTTTTCTCGCAACAGCAGGT 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 SerAlaMetGlyAlaArgAlaSerLeuThrValSerAlaGluSer 
               
               
                   
                 TCTGCAATGGGCGCTCGAGCGTCCCTGACCGTGTCGGCTCAGTGC 
               
               
                   
                   
               
               
                   
                     *         *      1600         *         * 
               
               
                   
                 ArgThrLeuLeuAlaGlyIleValGlnGlnGlnGlnGlnLeuLeu 
               
               
                   
                 CGGACTTTACTGGCCGGGATAGTGCAGCAACAGCAACAGCTGTTG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AspValValLysArgGlnGlnGluLeuLeuArgLeuThrValTrp 
               
               
                   
                 GACGTGGTCAAGAGACAACAAGAACTGTTGCGACTGACCGTCTGG 
               
               
                   
                   
               
               
                   
                          *         *         *      1700         * 
               
               
                   
                 GlyThrLysAsaLeuGlnAlaArgValThrAlaIleGluLysTyr 
               
               
                   
                 GGAACGAAAAACCTCCAGGCAAGAGTCACTGCTATAGAGAAGTAG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LeuGlnAspGlnAlaArgLeuAsnSerTrpGlyCysAlaPheArg 
               
               
                   
                 CTACAGGACCAGGCGCGGCTAAATTCATGGGGATGTGCGTTTAGA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         *      1800 
               
               
                   
                 GlnValCysHisThrThrValProTrpValAsnAspSerLeuAla 
               
               
                   
                 CAAGTCTGCCACACTACTGTACCATGGGTTAATGATTCCTTAGCA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 ProAspTrpAspAsnMetThrTrpGlnGluTrpGluLysGlnVal 
               
               
                   
                 CCTGACTGGGACAATATGACGTGGCAGGAATGGGAAAAACAAGTC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ArgTyrLeuGluAlaAsnIleSerLysSerLeuGluGlnAlaGln 
               
               
                   
                 CGCTACCTGGAGGCAAATATCAGTAAAAGTTTAGAACAGGCACAA 
               
               
                   
                   
               
               
                   
                       1900         *         *         * 
               
               
                   
                 IleGlnGlnGluLysAsnMetTyrGluLeuGlnLysLeuAsnSer 
               
               
                   
                 ATTCAGCAAGAGAAAAATATGTATGAACTACAAAAATTAAATAGC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 TrpAspIlePheGlyAsnTrpPheAspLeuThrSerTrpValLys 
               
               
                   
                 TGGGATATTTTTGGCAATTGGTTTGACTTAACCTCCTGGGTCAAG 
               
               
                   
                   
               
               
                   
                          *      2000         *         * 
               
               
                   
                 TyrIleGlnTyrGlyValLeuIleIleValAlaValIleAlaLeu 
               
               
                   
                 TATATTCAATATGGAGTGCTTATAATAGTAGCAGTAATAGCTTTA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ArgIleValIleTyrValValGlnMetLeuSerArgLeuArgLys 
               
               
                   
                 AGAATAGTGATATATGTAGTACAAATGTTAAGTAGGCTTAGAAAG 
               
               
                   
                   
               
               
                   
                          *         *      2100         * 
               
               
                   
                 GlyTyrArgProVaLPheSerSerProProGlyTyrIleGlu*** 
               
               
                   
                 GGCTATAGGCCTGTTTTCTCTTCCCCCCGCGGTTATATCCAATAG 
               
               
                   
                   
               
               
                   
                 IleHisIleHisLysAspArgGlyGlnProAlaAsnGluGluThr 
               
               
                   
                 ATCCATATCCACAAGGACCGGGGACAGCCAGCCAACGAAGAAACA 
               
               
                   
                   
               
               
                   
                          *         *         *      2200 
               
               
                   
                 GluGluAspGlyGlySerAsnGlyGlyAspArgTyrTrpProTrp 
               
               
                   
                 GAAGAAGACGGTGGAAGCAACGGTGGAGACAGATACTGGCCCTGG 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ProIleAlaTyrIleHisPheLeuIleArgGlnLeuIleArgLeu 
               
               
                   
                 GCGATAGCATATATACATTTCCTGATGCGCCAGCTGATTCGCCTC 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LeuThrArgLeuThrSerIleCysArgAspLeuLeuSerArgSer 
               
               
                   
                 TTGACCAGACTATACAGCATCTGCAGGGACTTACTATCAAGGAGC 
               
               
                   
                   
               
               
                   
                  2300         *         *         *         * 
               
               
                   
                 PheLeuThrLeuGlnLeuIleTyrGlnAsnLeuArgAspTrpLeu 
               
               
                   
                 GTCCTGACCCTCCAACTCATCTACCAGAATCTCAGAGACTGGCTG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 ArgLeuArgThrAlaPheLeuGlnTyrGlyCysGluTrpIleGln 
               
               
                   
                 AGACTTAGAACAGCCTTCTTGCAATATGGGTGCGAGTGGATCCAA 
               
               
                   
                   
               
               
                   
                     *      2400         *         *         * 
               
               
                   
                 GluAlaPheGlnAlaAlaAlaArgAlaThrArgGluThrLeuAla 
               
               
                   
                 GAAGCATTCCAGGCCGCCGCGAGGGCTACAAGAGAGACTCTTGCG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 GlyAlaCysArgGlyLeuTrpArgValLeuGluArgIleGlyArg 
               
               
                   
                 GGCGCGTGCAGGGGCTTGTGGAGGGTATTCGAACGAATCGGGAGG 
               
               
                   
                   
               
               
                   
                     *         *      2500         *         * 
               
               
                   
                 GlyIleLeuAlaValProArgArgIleArgGlnGlyAlaGluIle 
               
               
                   
                 GGAATACTCGCGGTTCCAAGAAGGATCAGACAGGGCGAAGAAATC 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AlaLeuLeu***GlyThrAlaValSerAlaGlyArgLeuTyrGlu 
               
               
                   
                 GGCCTCCTGTGAGGGACGGCAGTATCAGCAGGGAGACTTTATGAA 
               
               
                   
                   
               
               
                   
                     *         *         *      2600         * 
               
               
                   
                 TyrSerMetGluGlyProSerSErArgLysGlyGluLysPheVal 
               
               
                   
                 TACTCCATGCAAGGACCCAGCAGCAGAAAGGGAGAAAAATTTGTA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 GlnAlaThrLysTyrGly 
               
               
                   
                 CAGGCAACAAAATATGGA 
               
               
                   
                     *         * 
               
               
                   
                   
               
               
                   
                 MetGlyAlaArgAsnSerValLeuArgGlyLysLysAlaAspGlu 
               
               
                   
                 ATGGGCGCGAGAAACTCCGTCTTGAGAGGGAAAAAAGCAGATGAA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LeuGluArgIleArgLeuArgProGlyGlyLysLysLysTyrArg 
               
               
                   
                 TTAGAAAGAATCAGGTTACGGCCCGGCGGAAAGAAAAAGTACAGG 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 LeuLysHisIleValTrpAlaAlaAsnLysLeuAspArgPheGly 
               
               
                   
                 CTAAAACATATTGTGTGGGCAGCGAATAAATTGCACAGATTCGGA 
               
               
                   
                   
               
               
                   
                        100         *         *         * 
               
               
                   
                 LeuAlaGluSerLeuLeuGluSerLysGluGlyCysGluLysIle 
               
               
                   
                 TTAGCAGAGAGCCTGTTGGAGTCAAAAGAGGGTTGTCAAAAAATT 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 LeuThrValLeuAspProMetValProThrGlySerGluAsnLeu 
               
               
                   
                 CTTACAGTTTTAGATCCAATGGTACCGACAGGTTCAGAAAATTTA 
               
               
                   
                   
               
               
                   
                          *       200         *         * 
               
               
                   
                 LysSerLeuPheAsnThrValCysValIleTrpCyrIleHisAla 
               
               
                   
                 AAAAGTCTTTTTAATACTGTCTGCGTCATTTGGTGCATACAGGCA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 GluGluLysValLysAspThrGluGlyAlaLysGlnIleValArg 
               
               
                   
                 GAAGAGAAAGTGAAAGATACTGAAGGAGCAAAACAAATAGTGCGG 
               
               
                   
                   
               
               
                   
                          *         *       300         * 
               
               
                   
                 ArgHisLeuValAlaGluThrGlyThrAlaGluLysMetProSer 
               
               
                   
                 AGACATCTAGTGGCAGAAACAGGAACTGCAGAGAAAATGCCAAGC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ThrSerArgProThrAlaProSerSerGluLysGlyGlyAsnTyr 
               
               
                   
                 ACAAGTAGACCAACAGCACCATCTAGCGAGAAGGGAGGAAATTAC 
               
               
                   
                   
               
               
                   
                          *         *         *       400 
               
               
                   
                 ProValGlnHisValGlyGlyAsnTyrThrHisIleProLeuSer 
               
               
                   
                 CCAGTGCAACATGTAGGCGGCAACTACACCCATATACCGCTGAGT 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ProArgThrLeuAsnAlaTrpValLysLeuValGluGluLysLys 
               
               
                   
                 CCCCGAACCCTAAATGCCTGGGTAAAATTAGTAGAGGAAAAAAAG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 PheGlyAlaGluValValProGlyPheGlnAlaLeuSerGluGly 
               
               
                   
                 TTCGGGGCAGAAGTAGTGCCAGGATTTCAGGCACTCTCAGAAGGC 
               
               
                   
                   
               
               
                   
                   500         *         *         *         * 
               
               
                   
                 CysThrProTyrAspIleAsnGlnMetLeuAsnCysValGlyAsp 
               
               
                   
                 TGCACGCCCTATGATATCAACCAAATGCTTAATTGTGTGGGCGAC 
               
               
                   
                   
               
               
                   
                           *         *         *         * 
               
               
                   
                 HisGlnAlaAlaMetGlnIleIleArgGluIleIleAsnGluGlu 
               
               
                   
                 CATCAAGCAGCCATGCAGATAATCAGGGAGATTATCAATGAGGAA 
               
               
                   
                   
               
               
                   
                     *       600         *         *         * 
               
               
                   
                 AlaAlaGluTrpAspValGlnHisProIleProGlyProLeuPro 
               
               
                   
                 GCAGCAGAATGGGATGTGCAACATCCAATACCAGGCCCCTTAGCA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AlaGlyGlnLeuArgGluProArgGlySerAspIleAlaGlyThr 
               
               
                   
                 GCGGGGCAGCTTAGAGAGCCAAGGGGATCTGACATAGCAGGGACA 
               
               
                   
                   
               
               
                   
                     *         *       700         *         * 
               
               
                   
                 ThrSerThrValGluGluGlnIleGlnTrpMetPheArgProGlu 
               
               
                   
                 ACAAGCACAGTAGAACAACAGATCCAGTGGATGTTTAGGCCACAA 
               
               
                   
                   
               
               
                   
                 AsnProValProValGlyAsnIleTyrArgArgTrpIleGlnIle 
               
               
                   
                 AATCCTGTACCAGTAGGAAACATCTATAGAAGATGGATCCAGATA 
               
               
                   
                   
               
               
                   
                     *         *         *       800         * 
               
               
                   
                 GlyLeuGlnLysCysValArgMetTyrAsnProThrAsnIleLeu 
               
               
                   
                 GGATTGCAGAAGTGTGTCAGGATGTAGAACCCGACCAACATCCTA 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AspIleLysGlnGlyProLysGluProPheGlnSerTyrValAsp 
               
               
                   
                 GACATAAAACAGGGACCAAAGGAGCCGTTCCAAAGCTATGTAGAT 
               
               
                   
                   
               
               
                   
                     *         *         *         *       900 
               
               
                   
                 ArgPheTyrLysSerLeuArgAlaGluGlnThrAspProAlaVal 
               
               
                   
                 AGATTCTACAAAAGCTTGAGGGCAGAACAAACAGATCCAGCAGTG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LysAsnTrpMetThrGlnThrLeuLeuValGlnAsnAlaAsnPro 
               
               
                   
                 AAGAATTGGATGACCCAAACACTGCTAGTACAAAATGCCAACCCA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 AspCysLysLeuValLeuLysGlyLeuGlyMetAsnProThrLeu 
               
               
                   
                 GACTGTAAATTAGTGCTAAAAGGACTAGGGATGAACCCTACCTTA 
               
               
                   
                       1000         *         *         * 
               
               
                   
                 GluGluMetLeuThrAlaCysGlnGlyValGlyGlyProGlyGln 
               
               
                   
                 GAAGAGATGCTGACCGCCTGTCAGGGGGTAGGTGCGCCAGGCCAG 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 LysAlaArgLeuMetAlaGluAlaLeuLysGluValIleGlyPro 
               
               
                   
                 AAAGCTAGATTAATGGCAGAGGCCCTGAAAGAGGTCATAGGACCT 
               
               
                   
                   
               
               
                   
                          *      1100         *         * 
               
               
                   
                 AlaProIleProPheAlaAlaAlaGlnGlnArgLysAlaPheLys 
               
               
                   
                 GCCCCTATCCCATTCGCAGCAGCCCAGCAGAGAAAGGCATTTAAA 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 CysTrpAsnCysGlyLysGluGlyHisSerAlaArgGlnCysArg 
               
               
                   
                 TGCTGGAACTGTGGAAAGGAAGGGCACTCGGCAAGACAATGCCGA 
               
               
                   
                   
               
               
                   
                          *         *      1200         * 
               
               
                   
                 AlaProArgArgGlnGlyCysTrpLysCysGlyLysProGlyHis 
               
               
                   
                 GCACCTAGAAGGCAGGGCTGCTGGAAGTGTGGTAAGCCAGGACAC 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 IleMetThrAsnCysProAspArgGlnAlaGlyPheLeuGlyLeu 
               
               
                   
                 ATCATGACAAACTGCCCAGATAGACAGGCAGGTTTTTTAGGACTG 
               
               
                   
                   
               
               
                   
                          *         *         *      1300 
               
               
                   
                 GlyProTrpGlyLysLysProArgAsnPheProValAlaGlnVal 
               
               
                   
                 GGCCCTTGGGGAAAGAAGCCCCGCAACTTCCCCGTGGCCCAAGTT 
               
               
                   
                   
               
               
                   
                     *         *         *         *         * 
               
               
                   
                 ProGlnGlyLeuThrProThrAlaProProValAspProAlaVal 
               
               
                   
                 CCGCAGGGGCTCACACCAACAGCACCCCCAGTGGATCCAGCAGTG 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 AspLeuLeuGluLysTyrMetGlnGlnGlyLysArgGlnArgGlu 
               
               
                   
                 GATCTACTGGAGAAATATATGCAGCAAGCGAAAAGACAGACAGAG 
               
               
                   
                   
               
               
                   
                  1400         *         *         *         * 
               
               
                   
                 GlnArgGluArgProTyrLysGluValThrGluAspLeuLeuHis 
               
               
                   
                 CAGAGAGAGAGACCATACAAGGAAGTGACAGAGGACTTACTGCAC 
               
               
                   
                   
               
               
                   
                          *         *         *         * 
               
               
                   
                 LeuGluGlnGlyGluThrProTyrArgGluProProThrGluAsp 
               
               
                   
                 CTCGAGCAGGGGGAGACACCATACAGGGAGCCACCAACAGAGGAC 
               
               
                   
                   
               
               
                   
                     *      1500         *         *         * 
               
               
                   
                 LeuLeuHisLeuAsnSerLeuPheGlyLysAspGln 
               
               
                   
                 TTGCTGCACCTCAATTCTCTCTTTGGAAAAGACCAG 
               
               
                   
                          *         *         * 
               
             
          
         
       
     
     Example 6 
     Peptide Sequences Encoded By The ENV and GAG Qenes 
     The following coding regions for antigenic peptides, identified for convenience only by the nucleotide numbers of Example 5, within the env and gag gene regions are of particular interest. 
     
       
         
               
               
             
           
               
                   
                 env1 (1732-1809) 
               
               
                   
                   
               
               
                   
                                      ArgValThrAlaIleGluLysTyr 
               
               
                   
                                      AGAGTCACTGCTATAGAGAAGTAC 
               
               
                   
                 LeuGlnAspGlnAlaArgLeuAsnSerTrpGlyCysAlaPheArg 
               
               
                   
                 CTACAGGACCAGGCGCGGCTAAATTCATGGGGATGTGCGTTTAGA 
               
               
                   
                     *         *         *         *      1000 
               
               
                   
                 GlnValCys 
               
               
                   
                 CAAGTCTGC 
               
               
                   
                   
               
               
                   
                 env2 (1912-1983) 
               
               
                   
                   
               
               
                   
                                      SerLysSerLeuGluGlnAlaGln 
               
               
                   
                                      AGTAAAAGTTTAGAACAGGCACAA 
               
               
                   
                                              *         * 
               
               
                   
                 IleGlnGlnGluLysAsnMetTyrGluLeuGlnLysLeuAsnSer 
               
               
                   
                 ATTCAGCAAGAGAAAAATATGTATGAACTACAAAAATTAAATAGC 
               
               
                   
                  1940         *         *         *         * 
               
               
                   
                 Trp 
               
               
                   
                 TGG 
               
               
                   
                   
               
               
                   
                 env3 (1482-1530) 
               
               
                   
                   
               
               
                   
                 Pro ThrLysGluLysArgTyrSerSerAlaHisGlyArgHisThrArg 
               
               
                   
                 CCT ACAAAAGAAAAAAGATACTCCTCTGCTCACGGCAGACATACAAGA 
               
               
                   
                         *      1500         *         *         * 
               
               
                   
                   
               
               
                   
                 env4 (55-129) 
               
               
                   
                   
               
               
                   
                          CysThrGlnTyrValThrValPheTyrGlyValPro 
               
               
                   
                          TGCACCCAATATGTAACTGTTTTCTATGGCGTACCC 
               
               
                   
                               *         *         *         * 
               
               
                   
                 ThrTrpLysAsnAlaThrIleProLeuPheCysAlaThr 
               
               
                   
                 ACGTGGAAAAATGCAACCATTCCCCTGTTTTGTGCAACC 
               
               
                   
                        100         *         * 
               
               
                   
                   
               
               
                   
                 env5 (175-231) 
               
               
                   
                   
               
               
                   
                                                        AspAsp 
               
               
                   
                                                        GATGAT 
               
               
                   
                                                             * 
               
               
                   
                 TyrGlnGluIleThrLeuAsnValThrGluAlaPheAspAlaTrp 
               
               
                   
                 TATCAGGAAATAACTTTGAATGTAACAGAGGCTTTTGATGCATGG 
               
               
                   
                          *       200         *         * 
               
               
                   
                 AsnAsn 
               
               
                   
                 AATAAT 
               
               
                   
                   
               
               
                   
                 env6 (274-330) 
               
               
                   
                   
               
               
                   
                    GluThrSerIleLysProCysValLysLeuThrProLeuCys 
               
               
                   
                    GAGACATCAATAAAACCATGTGTCAAACTAACACCTTTATGT 
               
               
                   
                          *         *       300         * 
               
               
                   
                 ValAlaMetLysCys 
               
               
                   
                 GTAGCAATGAAATGC 
               
               
                   
                     *         * 
               
               
                   
                   
               
               
                   
                 env7 (607-660) 
               
               
                   
                   
               
               
                   
                                      AsnHisCysAsnThrSerValIle 
               
               
                   
                                      AACCATTGCAACACATCAGTCATC 
               
               
                   
                                       610         *         * 
               
               
                   
                 ThrGluSerCysAspLysHisTyrTrpAsp 
               
               
                   
                 ACAGAATCATGTGACAAGCACTATTGGGAT 
               
               
                   
                          *         *         * 
               
               
                   
                   
               
               
                   
                 env8 (661-720) 
               
               
                   
                   
               
               
                   
                                               AlaIleArgPheArg 
               
               
                   
                                               GCTATAAGGTTTAGA 
               
               
                   
                                                        * 
               
               
                   
                 TyrCysAlaProProGlyTyrAlaLeuLeuArgCysAsnAspThr 
               
               
                   
                 TACTGTGCACCACCGGGTTATGCCCTATTAAGATGTAATGATACC 
               
               
                   
                     *         *       700         *         * 
               
               
                   
                   
               
               
                   
                 env9 (997-1044) 
               
               
                   
                   
               
               
                   
                       LysArgProArgGlnAlaTrpCysTrpPheLysGlyLys 
               
               
                   
                       AAAAGACCCAGACAAGCATGGTGCTGGTTCAAAGGCAAA 
               
               
                   
                       1000         *         *         * 
               
               
                   
                 TrpLysAsp 
               
               
                   
                 TGGAAAGAC 
               
               
                   
                   
               
               
                   
                 env10 (1132-1215) 
               
               
                   
                   
               
               
                   
                       LysGlySerAspProGluValAlaTyrMetTrpThrAsn 
               
               
                   
                       AAAGGCTCAGACCCAGAAGTAGCATACATGTGGACTAAG 
               
               
                   
                               *         *         *         * 
               
               
                   
                 CysArgGlyGluPheLeuTyrCysAsnMetThrTrpPheLeuAsn 
               
               
                   
                 TGCAGAGGAGAGTTTCTCTACTGCAACATGACTTGGTTCCTCAAT 
               
               
                   
                          *         *      1200         * 
               
               
                   
                   
               
               
                   
                 env11 (1237-1305) 
               
               
                   
                   
               
               
                   
                                      ArgAsnTyrAlaProCysHisIle 
               
               
                   
                                      CGCAATTATGCACCGTGCCATATA 
               
               
                   
                                         *         *         * 
               
               
                   
                 LysGlnIleIleAsnThrTrpHisLysValGlyArgAsnValTyr 
               
               
                   
                 AAGCAAATAATTAACACATGGCATAAGGTAGGGAGAAATGTATAT 
               
               
                   
                          *         *         *      1300 
               
               
                   
                   
               
               
                   
                 gagl (991-1053) 
               
               
                   
                   
               
               
                   
                 AspCysLysLeuValLeuLysGlyLeuGlyMetAsnProThrLeu 
               
               
                   
                 GACTGTAAATTAGTGCTAAAAGGACTAGGGATGAACCCTACCTTA 
               
               
                   
                       1000         *         *         * 
               
               
                   
                 GluGluMetLeuThrAla 
               
               
                   
                 GAAGAGATGCTGACCGCC 
               
               
                   
                     *         * 
               
             
          
         
       
     
     Of the foregoing peptides, env1, env2, env3 and gag1 are particularly contemplated for diagnostic purposes, and env4, env5, env6, env7, env8, env9, env10 and env11 are particularly contemplated as protecting agents. These peptides have been selected in part because of their sequence homology to certain of the envelope and gag protein products of other of the retroviruses in the H6V group. For vaccinating purposes, the foregoing peptides may be coupled to a carrier protein by utilizing suitable and well known techniques to enhance the host&#39;s immune response. Adjuvants such as calcium phosphate or alum hydroxide may also be added. The foregoing peptides can be synthesized by conventional protein synthesis techniques, such as that of Merrifield. 
     It will be apparent to those skilled in the art that various modifications and variations can be made in the processes and products of the present invention. Thus, it is intended that the present application cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents. For convenience in interpreting the following claims, the following table sets forth the correspondence between codon codes and amino acids and the correspondence between three-letter and one-letter amino acid symbols. 
     
       
         
               
               
               
               
             
               
               
               
               
               
             
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                   
                   
                 AMINO ACID 
               
               
                   
                 DNA CODON 
                 AMINO ACID 3 LET. 
                 1 LET. 
               
               
                   
                   
               
             
          
           
               
                   
                 \2 
                  T   C   A   G  
                  T   C   A   G  
                 T C A G 
               
               
                 1 
                 \3 
               
               
                   
                 T 
                 TTT TCT TAT TGT 
                 PHE SER TYR CYS 
                 F S Y C 
               
               
                 T 
                 C 
                 TTC TCC TAC TGC 
                 PHE SER TYR CYS 
                 F S Y C 
               
               
                   
                 A 
                 TTA TCA TAA TGA 
                 LEU SER *** *** 
                 L S * * 
               
               
                   
                 G 
                 TTG TCG TAG TGG 
                 LEU SER *** TRP 
                 L S * N 
               
               
                   
                 T 
                 CTT CCT CAT CGT 
                 LEU PRO HIS ARG 
                 L P H R 
               
               
                 C 
                 C 
                 CTC TCC TAC TGC 
                 LEU PRO HIS ARG 
                 L P H R 
               
               
                   
                 A 
                 CTA CCA CAA CGA 
                 LEU PRO GLN ARG 
                 L P Q R 
               
               
                   
                 G 
                 CTG CCG CAG CGG 
                 LEU PRO GLN ARG 
                 L P Q R 
               
               
                   
                 T 
                 ATT ACT AAT AGT 
                 ILE THR ASN SER 
                 I T N S 
               
               
                 A 
                 C 
                 ATC ACC AAC AGC 
                 ILE THR ASN SER 
                 I T N S 
               
               
                   
                 A 
                 ATA ACA AAA AGA 
                 ILE THR LYS ARG 
                 I T K R 
               
               
                   
                 G 
                 ATG ACG AAG AGG 
                 ILE THR LYS ARG 
                 M T K R 
               
               
                   
                 T 
                 GTT GCT GAT GGT 
                 VAL ALA ASP GLY 
                 V A D G 
               
               
                 G 
                 C 
                 GTC GCC GAC GGC 
                 VAL ALA ASP GLY 
                 V A D G 
               
               
                   
                 A 
                 GTA GCA GAA CGA 
                 VAL ALA GLU GLY 
                 V A E G 
               
               
                   
                 G 
                 GTG GCG GAG GGG 
                 VAL ALA GLU GLY 
                 V A E G 
               
               
                   
               
             
          
           
               
                   
                 3 Letter 
                 1 Letter 
                 CODONS 
               
               
                   
                   
               
               
                   
                 ALA 
                 A 
                 GCT GCC GCA GCG 
               
               
                   
                 ARG 
                 R 
                 CGT CGC CGA CGC AGA AGG 
               
               
                   
                 ASN 
                 N 
                 AAT AAC 
               
               
                   
                 ASP 
                 D 
                 GAT GAC 
               
               
                   
                 CYS 
                 C 
                 TGT TGC 
               
               
                   
                 GLN 
                 D 
                 CAA CAG 
               
               
                   
                 GLU 
                 E 
                 GAA GAG 
               
               
                   
                 GLY 
                 G 
                 GGT GGC GGA GGG 
               
               
                   
                 HIS 
                 H 
                 CAT CAC 
               
               
                   
                 ILE 
                 I 
                 ATT ATC ATA 
               
               
                   
                 LEU 
                 L 
                 CTT CTC CTA CTG TTA TTG 
               
               
                   
                 LYS 
                 K 
                 AAA AAG 
               
               
                   
                 MET 
                 M 
                 ATG 
               
               
                   
                 PHE 
                 F 
                 TTT TTC 
               
               
                   
                 PRO 
                 P 
                 CCT CCC CCA CCG 
               
               
                   
                 SER 
                 S 
                 TCT TCC TCA TCG AGT AGC 
               
               
                   
                 THR 
                 R 
                 ACT ACC ACA ACG 
               
               
                   
                 TRP 
                 W 
                 TGG 
               
               
                   
                 TYR 
                 Y 
                 TAT TAC 
               
               
                   
                 VAL 
                 V 
                 GTT GTC GTA GTG 
               
               
                   
                 *** 
                 * 
                 TAA TAG TGA
This invention includes a peptide comprising the sequence CAFRQVC and an antigenic composition using the same. It also includes an in vitro method of detecting HIV-1 and HIV-2 antibodies in a sample using the peptide, and a kit for carrying out the method.