Comparison of four kinds of syphilis testing methods in blood donations
Objective To compare four kinds of syphilis examination methods,RPR,TP-ELISA,TPHA and TPPA and to choose a suitable model for screening syphilis in blood donors.Methods RPR and TP-ELISA were used to screen samples of blood donors for treponema pallidum(TP)infection.TPHA and TPPA as the confirm methods were further tested all samples of blood donors.Results Of 58586 samples of blood donors,RPR and TPHA positive samples were 289 and 331 respectively,and the positive coincidence rate was 81%(267/331).64 samples were undetected and 22 samples were false positive by TP-ELISA.The false-negative rate and false-postive rate of RPR methods were 0.11%,0.024% respectively.Of 18206 samples of blood donors,TP-ELISA and TPHA positive samples were 114 and 111 respectively,and the positive coincidence rate was 98.2%(109/111).2 samples were undetected and 5 samples were false positive by TP-ELISA.The false-negative rate and false-postive rate of TP-ELISA methods were 0.01%,0.027% respectively.TPHA and TPPA had no difference in test results.Conclusion The combination of TP-ELISA with TPHA or TPPA to detect blood donors is an effective model for screening syphilis in blood service center.