Nucleic acid quantitation
A method for measuring the amount of nucleic acid present in a sample, said method comprising <SL> <LI>a) dividing said sample into at least three different reaction wells, <LI>b) subjecting the contents of each well to an amplification reaction; <LI>c) halting the reaction in each well after a different number of amplification cycles; <LI>d) determining the amount of target sequence within each well; and <LI>e) using the results to calculate the amount of nucleic acid present in the initial sample. </SL> Particularly suitable apparatus for carrying out the method is also described.