Study on the Effects of Different Diluents on the Quality of Boar Sperm Stored at 4
[Objective]This study was designed to develop a method of liquid storage of boar sperm at 4 ℃ using the modified BF5 diluent with BSA and N-acetyl-D-glucosamine(mBF5).[Method]Boar sperm were stored in LEN,BF5 and mBF5 diluents at 4 ℃ for 5 days,and sperm motility,acrosome morphology,viability and ATP were examined according to preservation period.[Result]The results showed that there were no significant differences in sperm motility among the three diluents from 1 day to 2 days stored at 4 ℃.However,the sperm motility showed significant differences among the three diluents after 3 days stored at 4 ℃.The sperm motility in mBF5 diluent was higher than that in LEN and BF5 diluents after 3 days of storage.The percentage of normal acrosome in mBF5 and BF5 diluents was higher than that in LEN diluent from 1 day to 5 days stored at 4 ℃.However,the percentage of normal acrosome in mBF5 diluent was higher than that in BF5 diluent after 5 days stored at 4 ℃.The percentage of sperm viability in mBF5 diluent was higher than that in LEN and BF5 diluent from 1 day to 5 days stored at 4 ℃.The percentage of sperm viability declined steadily from 1 day to 5 days stored in the three different diluents,respectively.Sperm ATP in mBF5 diluent was higher than that in LEN and BF5 diluents from 1 day to 5 days of storage.Sperm ATP declined rapidly after 5 days of storage in the three different diluents,respectively.[Conclusion]It was confirmed that boar sperm could be stored in mBF5 diluent at 4 ℃ for 5 days and showed higher motility,normal acrosome,sperm viability and sperm ATP concentration compared with that stored in LEN and BF5 diluents.