Study on the Wx Allele Gene Sequences of Two Low Amylose Content Mutants of Oryza indica
Based on the Wx gene sequence of japonica rice,the Wx gene sequences of the two low-amylose mutants XLA-1 and XLA-2 of Oryza indica were detected by PCR and sequencing analysis.The main results showed that: There were many mutant sites among XLA-1,XLA-2 and Xianxiaozhan(the wild type),most of them were base change.There was a sigle nucleotide polymorphisms(SNP) in the 10th exon(+1 208 bp) of XLA-1 and XLA-2(Xianxiaozhan for the T,mutant for the C),codon TCT changed into CCT,which resulted in the substitution of a proline residue for serine.The substitution could alter the spatial structure of Wx protein,which may not be fully integrated on the starch granules,leading to the decreased of amylose content.The preliminary results indicated that the existence of Wx allelles affected amylose content in the two mutants.