Diagnostic value of MMP-2 and MMP-9 in urine for bladder cancer
Objective To assess the diagnostic value of MMP-2 and MMP-9 in urine for bladder cancer,and to study the relationship between MMP-2 and MMP-9 levels and the development of bladder cancer.Methods Urinary MMP-2 and MMP-9 levels were detected using enzyme linked immunosorbent assay(ELISA) in 101 patients with bladder cancer,eighty-eight patients with benign urological lesions and fifty-one healthy volunteers.MMP-2 and MMP-9 levels were compared in three groups,and the relationship in different tumor stage groups was evaluated.The optimal cut-offs of MMP-2 and MMP-9 were identified by using ROC curve.Accuracy,positive predictive value and negative predictive value were calculated at optimal cut-off.Results MMP-2 and MMP-9 levels in bladder cancer group were higher than those in benign urological lesions group and control(P0.01).MMP-2 and MMP-9 levels in grade Ⅱ and Ⅲ in bladder cancer were higher than those in grade Ⅰ(P0.01).The AUROC of MMP-2 was 0.854,and the optimal cut-off value was 2.4 ng/mg protein.Accuracy was 80.8%,sensitivity was 81.2%,and specificity was 81.3%.The AUROC of MMP-9 was 0.906,and the optimal cut-off value was 6.2 ng/mg protein.Accuracy was 87.5%,sensitivity was 85.1%,and specificity was 89.2%.Combination of MMP-2 and MMP-9 reached to accuracy 90.0%,sensitivity 98.0% and specificity 96.4%.Conclusion MMP-2 and MMP-9 in urine might have great diagnostic potentials for bladder cancer.