Development of Microsatellite Markers for Miiuy Croaker, Miichthys miiuy (Sciaenidae), and Their Application in Verifying Gynogenesis of Large Yellow Croaker, Pseudosciaena crocea , Induced with M. miiuy Sperm
A total of 30 microsatellite markers were isolated from miiuy croaker, Miichthys miiuy, and characterized. Of these, 24 were polymorphic with an average of 4.33 alleles per locus (range: 2–8). The observed (Ho) and expected (He) heterozygosities ranged from 0.0667 to 1.0000 (mean: 0.6361) and 0.3828 to 0.8139 (mean: 0.6443), respectively. The mean polymorphism information content (PIC) value was 0.576, ranging from 0.348 to 0.823. Eight loci deviated significantly from the Hardy–Weinberg equilibrium (HWE). Twelve of the 30 primer pairs amplified DNA sequences of large yellow croaker, Pseudosciaena crocea, and 10 were polymorphic. Except for MM007, sequence alignments of microsatellite flanking regions of the other 11 loci were highly similar between the two species. The gynogenetic P. crocea induced by the sperm of M. miiuy were verified using four cross-amplified primer pairs. No specific paternal bands were found in the offspring, indicating exclusive maternal inheritance.