FLUORESCENCE IMAGING DEVICE WITH TWO WAVELENGTH REFLECTION
The inventive device comprises a first light source and a first wavelength (?1) corresponding to an excitation wavelength (?Ex) of a fluorophore. The excitation wavelength (?Ex) and an emission wavelength (?Em) of the fluorophore delimit a predetermined interval (?Em). The device also comprises a second light source having a second wavelength (?2) offset with regard to the first wavelength (?1) in such a manner that it is outside said predetermined interval (?Em). The offset (?12) between the first (?1) and second (?2) wavelengths is between 30 nm and 100 nm. A camera is provided comprising a filter opaque to the first (?1) and second (?2) wavelengths and transparent to the emission wavelength (?Em) and to the wavelengths noticeably greater than the highest of the first (?1) and second (?2) wavelengths. The light sources and the camera are synchronized for alternately activating one of the light sources and enabling the camera to alternately acquire a fluorescence image and a background noise image.