Resuscitation with whole body cooling prevents multiple organ damage after hemorrhagic shock in rats
Whole body cooling (WBC) has been shown to improve survival in hemorrhagic shock (HS) rat studies. We hypothesized that induction of WBC would be beneficial for multiple organ damage (MOD) during HS resuscitation. Light anesthesia was induced with ketamine in rats, and spontaneous breathing was maintained. Rectal temperature (Tr) was monitored at about 36oC. HS was induced by blood withdrawal of 6ml/100g body weight during a 60-minute period, following by blood transfusion for a 30-minute period. Immediately after blood transfusion, rats were randomized into three groups of 10 rats each: group 1, untreated HS rats maintained at Tr 36.5oC; group 2, HS rats maintained to Tr 37.5oC (with 36oC water blanket temperature); and group 3, HS rats maintained to Tr 34oC (with 16oC water blanket temperature). Rats were controlled at each target Tr for 30 min. It was found that resuscitation with blood, but not WBC, induced significant multiple organ dysfunction, manifested by a high plasma level of aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, creatinine, and lactate dehydrogenase. In addition, HS rats received blood transfusion alone has significant MOD, evidenced by a high score of lung, kidney, or liver. The MOD caused by HS were significantly attenuated by WBC, in particular with the 16oC WBC. Our data indicate that resuscitation with WBC prevents multiple organ dysfunction or damage after acute HS in rats.