LONG LIVED CHIMERIC ANTIGEN RECEPTOR (CAR) EXPRESSING T CELLS FOR CANCER THERAPY
A method of generating an enriched population of T cells for use in adoptive immunotherapy applications includes the isolating CD4/CD8 T-cells from a biological sample of a subject, optionally genetically modifying the isolated CD4/CD8 T-cells to express single or multiple chimeric antigen receptors (CARs), and separating T cells having a CD45RAintCD45ROint phenotype from the optionally genetically modified isolated T cells.