Biological Conjugate between Antibody and ZnS Nanoparticles as Probe for Atomic Absorption Spectrophotometry Determination of Estriol
ZnS semiconductor nanocrystals (NCs) were prepared by ways from primary materials of ZnCl 2 and Na 2 S in water solution. Using the synthesized ZnS NCs, a polyclonal antibody-based ZnS-labelled immunosorbent assay for the determination of estriol (E 3 ) was developed with atomic absorption spectrophotometry (AAS) as a detector. An immunoaffinity column was applied to testify conjugation between antibody and ZnS NCs. The linear range for determination of estriol is 40.0~600.0 ng.mL -1 , and the limit of detection (LOD) is 10.0 ng.mL -1 . Some serum samples have been analyzed with satisfactory results which are in good agreement with those obtained using ELISA. This work suggests the potential application of NCs as biological probes and AAS as detector in nonisotopic immunoassay.