Study on the Interaction between Gadolinium(III) Aminopolycarboxlicacid Complex and Bovine Serum Album by Spectroscopic Methods
A novel gadolinium diethylenetriamine-N, N-bis (2-acetamide benzoic acid)-N, N-bisacetic acid complex has been synthesized and characterized by elemental analysis, infrared spectrum, ultraviolet spectroscopy and thermal analysis. The interaction between Gd 3+ and aminopolycarboxlicacid ligand was investigated by fluorescence titration. The binding constant of this complex is calculated to be 1.2018×10 4 L·mol -1 , and binding stoichiometry is 1:1. Fluorescence spectra was also used to study the interaction between Gd (III) complex and bovine serum album (BSA). The results showed that Gd (III) complex can effectively quench the intrinsic fluorescence of BSA via static quenching. According to Stern-Volmer equation and Linewerver-Burk equation, the binding constant is calculated to be 3.7491×10 4 L·mol -1 and binding site is about 1.0.