Pulmonary surfactant, process for its preparation and its pharmaceutical use
1. Lung surfactant, obtainable by (A) (A1) extraction of comminuted lung tissue or of an aqueous lung extract prepared with an isotonic solution of an electrolyte or of a mono- or disaccharide with a water-immiscible lipid solvent, (A2) separating off of undissolved components from the extract, (A3) separating off of the lipid solvent, (A4) extraction with a water-soluble alcohol, (A5) separating off of alcohol-insoluble components from the extract and (A6) separating off of the alcohol and obtaining of the active material or (B) (B1) centrifuging off the cell fragments from an aqueous lung extract prepared with an isotonic solution of an electrolyte or of a mono- or disaccharide at relatively low centrifugal accelerations, (B2) bringing into contact of the extract with an insoluble adsorbent, (B3) washing of laden adsorbent with water or acetone with removal of non-adsorbed material, (B4) desorption of the active material with an organic solvent or a solvent mixture and (B5) separating off of the solvent or solvents with obtaining of the active material.