Study on Preparation of Microencapsulation of Lutein by Doehlert Design
Objective: To optimize the lutein microencapsulation formulation by Doehlert design/response surface methodology.Methods:The Microencapsulation of lutein was prepared by combining high shear scattered method with spray-drying method.The effects of three independent variables including the proportion of core material and wall material,ratio of Arabic gum to malto dextrin and the solid content with were investigated by selecting the parameter of the entrapment efficiency and drug loading of lutein as evaluation parameters.Nonlinear mathematic models were used to estimate the relationship between independent and the dependent variables.Response surfaces were delineated according to the mathematic models,and optimum formulation was selected.Results:Second-order polynomial equation showed more predictable than other equations.According to the optimal conditions,the lutein entrapment efficiency and drug loading of the prepared lutein microencapsulation were 83.15%and1.88%.Conclusion:The Doehlert design is a feasible and convenient method to optimize the prescription of lutein microencapsulation.