Spatially resolved X-ray micro/nano-spectroscopy and imaging on the model organism Daphnia magna using laboratory and synchrotron sources
This work demonstrates and further develops spatially resolved laboratory and synchrotron X-ray spectroscopic and imaging experiments to answer specific ecotoxicological questions on the model organism Daphnia magna. The first phase of this work consisted of developing appropriate sample preparation procedures for this organism in order to be able to perform X-ray analyses on Daphnia magna subjected to ecotoxicological experiments. In the second phase, X-ray set-ups at different laboratories and synchrotron facilities were optimized and characterized in order to be able to perform the X-ray spectral imaging experiments of interest on Daphnia magna. Finally, various data reduction strategies were employed to process the vast amount of spectral data for evaluation and interpretation.