Effects of serum and coculture with goat cumulus cells on in vitro development of porcine embryos
To further improve the in vitro development systems of porcine embryos,we optimized the types of embryo culture media,the methods of serum treatment during embryo culture,the effects of coculture with cumulus cells on embryos development in vitro,using porcine parthenogenetic embryos as a model.The results demonstrated that porcine zygote medium-3(PZM3)was the suitable medium for in vitro culture of porcine embryos compared with Charles Rosenkrans 1(CR1aa).Cultured in PZM3from Day 0to 4and then added 10%fetal bovine serum from Day 4to 6,porcine embryos showed the higher developmental capacity,with the cleavage rates,blastocyst rates,hatched blastocyst rates and cell number of blastocysts of 95.1%,56.5%,27.3%and 79.3respectively.Based on the above,we cocultured porcine embryos with goat cumulus cells and further improved the hatched blastocyst rates to 36.6%,suggesting an efficient and stable in vitro development systems of porcine embryos was established preliminarily.