Effect of Stem Cell Factor on Ectogenesis of Preimplantation Embryo of Mice
In order to optimize in vitro culture system of embryo to improve development rate and quality,taking in vivo fertilization embryo,in vitro fertilization embryo and parthenogenetic activation as research object,the effect of different concentrations of stem cell factor on embryo culture in vitro were studied.The results showed that the cleavage rate and morula rate had no significant difference in KSOM,but the blastocyst rate and total blastocyst number derived from PA was less than IVN and IVF.Different concentrations of stem cell factor had no significant effect on cleavage rate,morula rate,blastocyst rate and total blastocyst number.When the concentration of stem cell factor was 100ng·mL-1,the morula rate and blastocyst rate were significantly higher than CK.