Genetic Diversity of Different Geographic Populations in Perinereis aibuhitensis by ISSR and COI Markers
ISSR molecular markers and CO I gene sequence were used to identify the genetic diversity and structure of 7 geographic populations in Perinereis aibuhitensis,which were sampled from Dalian( DL),Rushan( RS),Panjin( PJ),Qingdao( QD),Yancheng( YC),Jinjiang( JJ) and west coast of North Korea( CX). The ISSR results showed that there was high genetic diversity between the 7 geographic populations of P. aibuhitensis. 116 bands were amplified with 10 ISSR primers with 109 polymorphic bands. The total percentage of polymorphic loci was 93. 97%. Nei's gene diversity was 0. 365 2,Shannon's information index was 0. 518 6. The coefficient of gene differentiation( Gst) was 0. 246 0. These data indicated that among-population component accounted for 24. 60% of the total variation,while the within-population component accounted for 76. 40%. The within-population genetic variation was larger than among-population. COI gene sequence was analyzed employing 70 individuals from 7 populations. There were 68 variable sites and 47 parsimony-information sites out of the total 469 sites. In 68 variable sites,there were 62 transition sites and 6 transversion sites,parsimony-information sites rate Pi was 9. 611%. Phylogenetic tree analysis showed that there was high genetic diversity within the tested population,which was accord with the result of ISSR. This result has provided theoretical basis for effective utilization and protection of P. aibuhitensis resources.