Preparation of O-Carboxymethyl Chitosan by Schiff Base and Antibacterial Activity
A novel method of preparation O-Carboxymethyl chitosan (OCMC) was studied that the product was prepared by reaction of Schiff's base of chitosan. Schiff base of chitosan (BCTS) was synthesized by the reaction of chitosan with aromatic aldehyde, then BCTS reacted with chloroacetic acid and removed the group of amino protection to get the target product. The chitosan derivative was characterized by FT-IR spectroscopy, 1HNMR and elemental analysis. Elemental analysis results confirmed that the degree of substitution(DS) of OCMC was 0.83. Solubility of OCMC in water and organic solvents was demonstrated to be better than that of chitosan. The antimicrobial activities of chitosan and OCMC were investigated against Aspergillus niger and Staphylococcus aureus. The results indicate that the antimicrobial activity of OCMC was superior to chitosan.