Bioinorganic intracellular synthesis of photo-responsive bio-camouflaged nanomedicines
Exposed to smart man-engineered nanomedicines, cells can profoundly transform and recycle them. 
To communicate, or in response to exogenous stress, cells generate extracellular vesicles which can play crucial roles in multiple physiological processes, as for instance, and unfortunately, for cancer progression. 
The goal of this proposal is to combine both biological events to deliver nanomedicines created by cells only, using mesenchymal stem cells as the most relevant cell model.
The focus of this proposal on nano-bio-production is three-fold. 
-	In the first instance, I aim to rationally develop novel strategies for bioinorganic intracellular synthesis of nanoparticles. The first aspect will be to demonstrate, as suggested by preliminary data, that nanoparticles degradation products can be de novo-synthesized nanoparticles! And also that nanoparticulate end products can also be obtained when cells are “fed” with soluble ionic salts. The vicarious synthesis using living cells as bioreactors, will allow creating functional, sophisticated and organism-friendly nanoparticles. Basic ingredients and little chemical proceedings should lead to advanced prospectively biological nano-tools. When iron and cobalt constituents will be used as precursors, cells will generate nanoparticles with magnetic and photothermal properties. 
-	The second focus is devoted to nanoparticles biological encapsulation. Indeed, in order to be useful, nanoparticles should be efficiently delivered to the target site. In the strategy to make “100% Bio Made nanomedicines”, it is obvious there are no better delivery systems than the cell’s innate delivery systems, the extracellular vesicles, an especially the ones derived from stem cells, supposed to inherit from their natural tumour homing. Here, ground-breaking physically-inspired (hydrodynamic, light) approaches for production and loading of these vesicles with nanoparticles will be proposed and fully implemented. One specific aim wi