Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1
Neurogenin 1 (ngn1), with the functions of controlling the differentiation of neurons, determining specific neuronal subtype, and inhibiting glial differentiation, is quantitatively detected for the first time. By using specifically modified silver nanoparticles (AgNP) as the signaling element, a label-free, rapid and sensitive colorimetric immunoassay for the synthetic peptide fragment of ngn1 (amino acid sequence: AQDDEQERRRRRGRTR) is reported. The detection procedure is based on an anti-aggregation mechanism, by which ngn1 inhibits the aggregation of the probe in the presence of salt (NaClO(4)). The anti-ngn1 antibody conjugated AgNP (denoted as AgNP-Ab) is negatively charged, and mono-binding of the like-charged ngn1 to the probe will increase the surface charge density, hence enhancing the interparticular electrostatic repulsion. Along with the increase of ngn1 concentration, the color of the solution varies from red to yellow, thereby developing a feasible approach for the detection of ngn1. Using a UV/vis spectrophotometer, this assay exhibits a linear response range of two orders of magnitude, from 50 to 800 ng mL(-1), and a detection limit of 30 ng mL(-1). On the basis of these qualities, the antibody-conjugated AgNP may become a useful tool for point-of-care diagnosis of ngn1 and such a method offers a new insight on the detection of the analogous antigen fragment as well.