HYBRID NANOPORE DEVICE WITH OPTICAL DETECTION AND METHODS OF USING SAME
Devices or systems comprising a protein nanopore immobilized in a lipid layer within an aperture of a solid phase substrate, which provides a stable platform for using first and second members of one or more FRET pairs to generate optical signals as a labeled analyte, such as a polynucleotide, translocates through the bore of the protein nanopore, are provided. In certain variations, the device or system may be used to determine the nucleotide sequence of a polynucleotide analyte.