SPECIES OF MRFFT1 CELL
The Invention relates to a MRFFT1 cell and a preparation method thereof. The Invention screens out mutant polypeptides with prediction of antigenic determinants, and links and synthesizes the expression gene sequences of mutant polypeptides. Meanwhile, the MVA virus vector is constructed to package MVA virus, and APC cells are transfected to complete the modification of specific MV cells, and PBMCs isolated from peripheral blood are co-cultured in vitro to screen out the effective polypeptide, and ordinary T cells is transformed into RFF cells with more precise killing through the second pulse of precise effective polypeptide stimulation. Then, the principle of TCR-T technology is used to modify the T cells, and gene coding technology is used to knock out the immunosuppressive target of the modified T cells. The specific killing T cells are precisely protected from in vivo inhibition, and the cytotoxicity of T cells to tumor cells is improved.