Preparation of enzyme linked immunosorbent assay kit for milk cow progesterone.
In order to investigate the change in progesterone level of cow to provide reference on heat and gestation period identification,and ovary disease diagnosis,the enzyme linked immunosorbent assay kit(ELISA) for cow progesterone was prepared based on primary mechanism of ELISA.The results showed that applying progesterone bovine serum albumin(P4-BSA) cross-linking polymer could solve the problem of enveloping antigen on ELISA plate,and antigen was easy to be conserved.It was found that high titer antibody could be obtained by applying progesterone-ovalbumin(P4-OVA) cross-linking polymer to immunize the rabit and it could block 1 ng/mL progesterone.The 2000 and 500 dilution times for P4-BSA and serum were found to be the optimal concentration of kit by ELISA chessboard method.The cow dung was used for detecting sample with kit,and the change in progesterone level of dung could provide reference on heat and gestation period identification,and diagnosis of reproduction barrier disease.