FAST RECOMPENSATION OF FLOW CYTOMETERY DATA FOR SPILLOVER READJUSTMENTS
n one embodiment, a method of performing fast compensation in a flow cytometry experiment is provided. The method includes the following: generating an initial spillover matrix by using a plurality of single stained compensation controls; running a sample through the flow cytometer; generating a measured sample event vector by measuring fluorescence of a plurality of cells passing through the flow cytometer; generating a compensated sample event vector by using the initial spillover matrix and the measured sample event vector; generating an adjusted spillover matrix by finely adjusting the initial spill-over matrix; and calculating a re-compensated event vector by using the adjusted spillover matrix and the measured sample event vector.