Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba
The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue on
their follicles morphology. The study was carried out in two experiments. Experiment I, ovaries were
maintained in Phosphate Buffered Saline (PBS) at -20o
C and room temperature (RT) for 24 h, and at 5o
C
for 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, the
ovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loaded
into hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) at
room temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred to
vitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placed
directly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All of
the follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicles
was significantly reduced when ovarian tissues were stored at -20o
C for 24 h and at 5o
C for 24 h and 72 h.
However, it seemed to have a minor deterioration effect when the tissues were kept at 5o
C for 24 h
(P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved their
morphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10
minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared to
when tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposing
tissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphology
in good condition