Optimization for prescription of Baoxieling Hydrogel Patch using Box-Behnken test design and research on its transdermal permeability in vitro
Objective To optimize the prescription of Baoxieling Hydrogel Patch(BHP) using Box-Behnken test design and to investigate its transdermal absorption properties in vitro. Methods Taking the comprehensive scores of the early adhesion, uniformity, ductility, consistence, skin adhesive ability, repeated exposing paste, and residue as response values, Box-Behnken test design was used to optimize the amounts of sodium polyacrylate NP 800, aluminum glycinate, and fillers, and to validate the optimal formulation. The percutaneous permeation of cinnamaldehyde, eugenol, evodiamine, and rutaecarpine in the optimal formulation was studied by in vitro transdermal delivery experiment with Franz diffusion cells and their contents were determined by HPLC. Results The optimal ratio of the prescription was as follows: sodium polyacrylate NP 800-aluminum glycinate-fillers(0.82∶0.02∶1.56). The foremost factors were fillers and aluminum glycinate. Its transdermal absorption met zero order dynamic process. Conclusion The optimal prescription has uniform paste, suitable consistence, easy ductility, moderate adhesion, and perfect transdermal effect. It could provide the foundation for the development of new prescription of Baoxieling.