On the different lipolytic capability of diverse organs from young adult guinea pigs. A chromatographic study
In this study we conducted in-vitro incubation of whole-tissue homogenate of young adult guinea pig heart, kidney, testis, and spleen at pH 7.4 and 38°C for 60 min. Subsequent TLC analysis revealed: (a) a noticeably high level of MLCL and a very low level of CL in control testis, indicating possible in-vivo deacylation of CL (b) a high level of CL in control guinea pig heart and kidney samples, and no MLCL detected (c) MLCL was only produced (in both heart and kidney) subsequent to in-vitro incubation (d) a very low level of CL was present in control samples of guinea pig spleen and no MLCL was produced subsequent to in-vitro incubation (e) guinea pig heart contains both ethanolamine (PE) and choline plasmalogens (PC) (f) PE plasmalogen is the only alkenyl species present in guinea pig kidney, testes, and spleen (g) sphingomyelin (SM) is uniquely higher in kidney than testis and spleen and very low in the heart (h) subsequent to in-vitro incubation, ceramide is produced in the kidney and testes only, with...