{"content":[{"text":"Am. J. Pharm. & Toxicol., 4 (4): 118-126, 2009","bbox":{"left":0.343921,"top":0.090314,"width":0.312239,"height":0.015355000000000008}},{"text":"quercetin 3-O-beta-d-glucopyranosyl-(2-1)-O-beta-d- flavonoids, steroids, terpenoids, tannins, resins and \n xylopyranoside, beta sitosterol, gallic acid[16], the glycosides. \n lignans- phyllanthin, hypophyllanthin, phyltetralin with \n antihyperuricemic[18] Wound healing activity The \n and antihepatotoxic effects[4], studies: effect of ME on \n wound healing was studied in rodents using \n cubebin dimethyl ether, urinatetralin[44], niranthin[45], \n experimentally-created excision and dead space \n methyl brevifolincarboxylate with vasorelaxant[1] and \n wounds. \n platelet aggregation inhibitory[30] effects, niruriside with \n HIV/RRE binding inhibitory effect[34], the prenylated \n Excision wound: Animals randomly placed into four \n flavanones- 8-(3-Methyl-but-2-enyl)-2-phenylchroman- \n groups (I-IV; n = 6) were anaesthetized with \n 4-one and 2-(4-hydroxyphenyl)-8-(3-methyl-but-2- \n chloroform and excision wounds (2.5 cm diameter) \n enyl)-chroman-4-one with nematicidal activity[29], \n created on the depilated dorsal thoracic region[48]. \n triacontanal and tricontanol with antihepatotoxic \n Groups I and II received ME (5 or 10%) ointment while \n effect[4] and xylans[3]. \n III and IV were treated with neomycin-bacitracin (5%) \n In continuation of efforts to elucidate the \n or vehicle (ointment base) respectively. The ointments \n pharmacological activities and hence medicinal \n were topically applied once daily from day 1, after \n potential of this plant, we studied the wound healing \n wound creation and continued until the wound healed. \n and antiulcer properties of extract of the aerial parts \n The wound diameter was measured on alternate days \n using experimentally created wounds and ulcers in \n and the epithelialization period recorded at the end of \n rodents. \n the study. Wound contraction (%) was calculated using \n the relation: \n MATERIALS AND METHODS \n Wound contraction (%) = [(WD0-WDt)/WD0] 100 \n Animals: Adult albino rats (150-200 g) of either sex \n obtained from the Department of Pharmacology and \n Where: \n Toxicology, University of Nigeria Nsukka were used. \n WD0 = The wound diameter on day zero \n The animals were housed in metal cages and \n WDt = The wound diameter on day t \n maintained on standard pelleted rodent diet and water \n ad libitum. All animal experiments were in compliance \n The time taken for 50% of wound closure (WC50) \n with the National Institute of Health Guide for Care and \n was read off a plot of wound closure (%) against time \n Use of Laboratory Animals (Pub No. 85-23, revised \n (days). \n 1985). \n Dead space wound: Dead space wounds were created \n Drugs: Neomycin-bacitracin powder (Cicatrin(R)). \n by making a pouch through a small cut and implanting \n sterile cotton pellets (50 mg each) on either side of the \n Reagents: Chloroform, methanol, dimethylsulfoxide \n axilla on the ventral surface of each rat. The animals \n (DMSO), ethanol. \n were randomly divided into three groups (n = 6). \n Groups I and II received 200 or 400 mg kg−1 of ME \n Preparation and extraction of plant material: Fresh \n suspended in DMSO (10%) respectively. Group III \n whole P. niruri plants were collected from Orba, Enugu \n served as control and received the vehicle. Extract \n State in May 2008 and authenticated by Mr. Alfred \n administration was done orally once a day from day 0- \n Ozioko of the International Centre for Ethnomedicine \n 9. On day 10, the animals were sacrificed by overdose \n and Drug Development (InterCEDD) Nsukka, Nigeria. \n of chloroform anaesthesia and the pellets carefully \n The plants were thoroughly cleaned and the aerial parts \n removed and dried in an oven at 60°C to a constant \n cut into smaller pieces, sun-dried for 7 days and milled \n weight and the weight recorded. The level of increase \n to coarse powder using a mechanical grinder. The \n (%) in the weight of granuloma tissue formed was \n powdered plant material (2 kg) was extracted with \n calculated relative to the control. \n methanol by continuous extraction in a soxhlet. \n Concentration of the extract in a rotary evaporator Antiulcer activity studies: The ulcer-healing or \n under reduced pressure afforded 140.6 g of the protective effect of the extract was studied in rodents \n methanol extract (ME; 7.03% w/w). Phytochemical using ulcers induced by different ulcerogens. \n analysis for identification of constituents using \n conventional methods[46,47] gave positive reactions for Indomethacin-induced ulcers: Animals were fasted \n alkaloids, saponins, carbohydrates, reducing sugar, for 18 h but allowed access to water only prior to the \n 119","bbox":{"left":0.117646,"top":0.118122,"width":0.764861,"height":0.7913209999999999}}],"images_bbox":[],"images_bbox_not_text_overlap":[]}