{"content":[{"text":"Asymmetric-Flow Field-Flow Fractionation AF4 \n https://labfacilities.wur.nl/SearchDetail.aspx?deviceid=05bff07b-217e-4d62-aa07-8d2feae1a526","bbox":{"left":0.053782,"top":0.135998,"width":0.711378,"height":0.044346}},{"text":"Brand \n Wyatt/Dionex","bbox":{"left":0.053782,"top":0.194796,"width":0.099933,"height":0.038017999999999996}},{"text":"Type","bbox":{"left":0.053782,"top":0.247254,"width":0.054918,"height":0.022979}},{"text":"Contact \n Rene Kuijpers (rene.kuijpers@wur.nl)","bbox":{"left":0.053782,"top":0.283097,"width":0.27949500000000005,"height":0.043957000000000024}},{"text":"Organisation \n Agrotechnology & Food Sciences Group","bbox":{"left":0.053782,"top":0.341494,"width":0.29986500000000005,"height":0.03801899999999997}},{"text":"Department \n Food Chemistry","bbox":{"left":0.053782,"top":0.393964,"width":0.130729,"height":0.038019000000000025}},{"text":"Description \n Asymmetric flow field flow fractionation (AF4) an analytical technique to separate larger molecules, aggregates and \n particles based on their size. It is an interesting alternative for size-exclusion chromatography (SEC). \n The asymmetric flow field flow fractionation (AF4) is a technique to separate larger molecules (e.g. proteins, \n polysaccharides), aggregates (e.g. heat induced protein aggregates) and particles based on their size. This separation \n is obtained by the difference in mobility in the flow field induced by the liquid flow over the membrane and across the \n channel. AF4 can thus be used for separation of various sizes in a mixutre and obtaining their solution of physical \n properties. The set-up has a number of different detectors, together allowing the determination of: \n • molar mass \n • root mean squared radius (of gyration) \n • second virial coefficient of the eluting compounds \n Information obtained by AF4 analysis provides information on the diffusion coefficients, hydrodynamic radius and the \n shape of the aggregates. \n Benefits of the AF4 over size-exclusion column chromatography are: \n (1) the ability to separate both soluble and colloidal components over a wide size range, and (2) that in AF4 there is little \n or no specific interaction that can affect the elution of the samples (as no column material is used).","bbox":{"left":0.053782,"top":0.446423,"width":0.890234,"height":0.22804200000000008}},{"text":"Technical Details \n The AF4 system consists of multi angle laser light scattering (MALLS from Wyatt, Dawn Heleos-II), differential refractive \n index cell (dRI from Wyatt, Optilab T-rex) and UV absorbance cell (DAD, Diode Array Detector from Dionex). The AF4 \n system can be used with both the flat plate and hollow fibre cylindrical channel type flow cells. The flow conditions and \n data collection is controlled by Wyatt Eclipse separation system- Dual Tec. Eclipse is coupled with Dionex ultiMate 3000 \n UHPLC, which contains pump, auto sampler, column compartments and DAD. \n AF4 results have been shown for the elution of single, monomeric proteins (e.g. bovine serum albumin, 66 kDa), larger \n proteins (Keyhole Limpet Hemocyanin 8.3 MDa), as well as silica particles (41-400 nm), illustrating the broad range of \n molecular weight / particle size that can (simultaneously) be analysed.","bbox":{"left":0.053782,"top":0.688917,"width":0.890151,"height":0.13302999999999998}}],"images_bbox":[{"left":0.05042,"top":0.035629,"width":0.357361,"height":0.055238},{"left":0.589916,"top":0.191045,"width":0.168067,"height":0.079204},{"left":0.769748,"top":0.191045,"width":0.168067,"height":0.079026}],"images_bbox_not_text_overlap":[{"left":0.05042,"top":0.035629,"width":0.357361,"height":0.055238},{"left":0.589916,"top":0.191045,"width":0.168067,"height":0.079204},{"left":0.769748,"top":0.191045,"width":0.168067,"height":0.079026}]}