 DEAS  1119-3: 202 3 
ICS 7 1.100.7 0 , 65.100  
 
© EAC 2023                                                                                                                               First Edition 2023   
 DRAFT EAST AFRICAN STANDARD  
Skin applied mosquito repellents — Specification — Part 3: Wipes  
EAST AFRICAN COMMUNITY  
 
 
 
 
 
 DEAS  1119 -3: 2023  
ii © EAC 2023  – All rights reserved  
 Copyright notice  
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Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be pr osecuted . DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ .............................  v 
1 Scope  ................................ ................................ ................................ ................................ ......................  7 
2 Normative references  ................................ ................................ ................................ ............................  7 
3 Terms and definitions  ................................ ................................ ................................ ...........................  8 
4 Symbols and/or abbreviated terms  ................................ ................................ ................................ ..... 9 
5 Active ingredients  ................................ ................................ ................................ ................................ . 9 
5.1 Natural repellents  ................................ ................................ ................................ ................................ .. 9 
5.2 Synthetic repellents  ................................ ................................ ................................ ............................  10 
6 Requirements  ................................ ................................ ................................ ................................ ....... 10 
6.1 General requirements  ................................ ................................ ................................ .........................  10 
6.2 Specific requirements  ................................ ................................ ................................ .........................  10 
6.3 Microbiological  requirements  ................................ ................................ ................................ ............  11 
6.4 Biological  efficacy  ................................ ................................ ................................ ...............................  12 
7 Packaging  ................................ ................................ ................................ ................................ .............  12 
8 Labelling  ................................ ................................ ................................ ................................ .................  1 
8.1 Primary  packaging  ................................ ................................ ................................ ................................  1 
8.2 Secondary  packaging  ................................ ................................ ................................ ...........................  1 
9 Sampling  ................................ ................................ ................................ ................................ ................  1 
Annex  A (normative)  Determination of DEET content  ................................ ................................ ...................  2 
A.1 General  ................................ ................................ ................................ ................................ ...................  2 
A.2 Apparatus  ................................ ................................ ................................ ................................ .....................  2 
A.3 Preparation of calibration curve  ................................ ................................ ................................ ..........  2 
A.4 Proc edure  ................................ ................................ ................................ ................................ ...............  2 
A.5 Calculation  ................................ ................................ ................................ ................................ .............  3 
Annex  B (normative)  Determination of ethyl butylacetamidopropionate (IR3535).  ................................ .... 4 
B.1 Outline of method  ................................ ................................ ................................ ................................ .. 4 
B.2 Reagents  ................................ ................................ ................................ ................................ ................  4 
B.3 Apparatus  ................................ ................................ ................................ ................................ ...............  4 
B.4 Procedure  ................................ ................................ ................................ ................................ ...............  4 
B.4.1  Chromatographic conditions (typical)  ................................ ................................ ................................  4 
B.5 Preparation of sample . ................................ ................................ ................................ ..........................  5 
B.6 System equilibration  ................................ ................................ ................................ .............................  5 
B.7 Determination.  ................................ ................................ ................................ ................................ ....... 5 
B.8 Calculat ion ................................ ................................ ................................ ................................ .............  6 
Annex C  (normative)  Determination of icaridin  ................................ ................................ .............................  7 
C.1 outline of method  ................................ ................................ ................................ ................................ .. 7 
C.2 Reagents  ................................ ................................ ................................ ................................ ................  7 
C.3 Apparatus  ................................ ................................ ................................ ................................ ...............  7 
C.4 procedure  ................................ ................................ ................................ ................................ ...............  7 
C.4.1 Chromatographic conditions (typical)  ................................ ................................ ................................  7 
C.5 Preparation of sample  ................................ ................................ ................................ ...........................  8 
C.6 Equilibration of the system  ................................ ................................ ................................ ..................  8 
C.7 Determination  ................................ ................................ ................................ ................................ ........  8 
C.8  Calculation  ................................ ................................ ................................ ................................ ............  8 
Annex D (normative)  Determination of total pyr ethrins  ................................ ................................ ..............  10 
D.1 General  ................................ ................................ ................................ ................................ .................  10 DEAS  1119 -3: 2023  
iv © EAC 2023  – All rights reserved  
 D.2 Reagents  ................................ ................................ ................................ ................................ ..............  10 
D.3 Apparatus  ................................ ................................ ................................ ................................ ............  10 
D.4 Operating conditions  ................................ ................................ ................................ ..........................  10 
D.5 Preparation of the standard  ................................ ................................ ................................ ...............  10 
D.6 Sample pre paration  ................................ ................................ ................................ ............................  11 
D.7 Procedure  ................................ ................................ ................................ ................................ ............  11 
D.8 Calculation of the % total pyrethrins (active ingredient)  ................................ ................................  11 
Annex E (normative)  Determination of absorbency rate  ................................ ................................ ............  12 
E.1 Apparatus  ................................ ................................ ................................ ................................ ............  12 
E.2 Preparation  of test specimens  ................................ ................................ ................................ ...........  12 
E.3 Procedure  ................................ ................................ ................................ ................................ ............  12 
D.4 Calculation  ................................ ................................ ................................ ................................ ...........  12 
Annex F  (normat ive) Determination  of moisture  content  ................................ ................................ ............  13 
F.1 Principle  ................................ ................................ ................................ ................................ ...............  13 
F.2 Apparatus  ................................ ................................ ................................ ................................ ............  13 
F.3 Sample  preparation  ................................ ................................ ................................ ............................  13 
F.4 Procedure  ................................ ................................ ................................ ................................ ............  13 
F.5 Calculation  ................................ ................................ ................................ ................................ ...........  13 
Annex G (normative)  Determination  of length  and width  ................................ ................................ ...........  15 
G.1Apparatus  ................................ ................................ ................................ ................................ ...................  15 
G.2 Procedure  ................................ ................................ ................................ ................................ ............  15 
G.2.1  Procedure for  width  ................................ ................................ ................................ ............................  15 
G.2.2  Procedure  for length ................................ ................................ ................................ ...........................  15 
Bibliography  ................................ ................................ ................................ ................................ .....................  16 
 DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  v 
 Foreword  
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized sta ndardiza tion, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee  (EASC)  mandated to develop and 
issue East African Standards  (EAS). The Committee is composed of representatives of the National 
Standards Bodies in Partner States, together with the representatives from the public and private sector 
organizations  in the community .  
East African Standards are developed through  Technica l Commit tees that are representative of key 
stakeholders including government, academia, consumer groups, private sector and other interested parties. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated before finalization of standards, in 
accordance with the Principles and procedures for development of East African Standards. XXXXXX.  
East African Standards are subject to review, to keep  pace wi th technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
The committee responsible for this document is Technical Committee EA SC/TC 07 8, Healthcare and medical 
devices.  
Attention is drawn to the possibility that some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights.  
DEAS 1119 consists  of the following parts, under the general title Skin applied mosquito repellents —
Specification:  
Part 1: Lotions, creams, gels and ointments  
Part 2: Sprays and roll -ons 
Part 3: Wipes  
Part 4: Bathing soaps  
Part 5: Bracelets, wristbands and patche s 
Part 6: Jelly   
DRAFT EAST AFRICAN STANDARD  DEAS  1119 -3:2023 
 
© EAC  2023  – All rights  reserved  7 
 Skin applied mosquito repellents — Specification — Part 3: Wipes  
1 Scope   
This Draft East Africa Standard specifies requirements, sampling and test methods for skin applied mosquito repellent 
wipes  
2 Normative refere nces   
The following documents  are referred to in the text in such a way that some or all of their con tent constitutes requirements 
of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the 
referenced document (including a ny amendments) applies.  
DEAS 1120 -1, Mosquito repellent — Performance te st guidelines — Part 1: Skin applied  
EAS 846, Glossary of terms relating to the cosmetic industry  
ISO 139, Textiles — Standard atmospheres for conditioning  and testing  
ISO 1833 -1, Textiles — Binary fibre mixtures — Quantitative chemical analysis  
ISO 3071, Textiles — Determination of pH of aqueous extract  
ISO 9073 -1, Textiles — Test methods for nonwovens — Part 1: Determination of mass per unit area  
ISO 9073 -18, Textiles — Test methods f or nonwovens — Part 18: Determination of breaking strength and  elongatio n of 
nonwoven materials using the grab tensile test  
ISO 18416, Cosmetics — Microbiology — Detection of Candida albicans  
ISO 20743, Textiles — Determination  of antibacterial activity of  textile products  
ISO 21149, Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria  
ISO 21150, Cosmetics — Microbiology — Detection of Escherichia coli  
ISO 22717, Cosmetics — Microbiology — Detectio n of Pseudomonas aeruginosa  
ISO 22718, Cosmetics — Microbiology — Detection of Staphylococcus aureus  DEAS  1119 -3: 2023  
8 © EAC 2023  – All rights reserved  
 ISO 2859 -1, Sampling procedures for inspection by attributes — Part 1: Sampling schemes indexed by  acceptance quality 
limit (AQL) for lot -by-lot inspection  
3 Terms and definitions   
For the purposes of this document, the following terms and definitions give n in EAS 846 and the following apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:   
— ISO Online bro wsing platform: available a t http://www.iso.org/obp  
3.1  
mosquito  
blood -sucking dipterous insect of the family Culicidae. Aedes, Anopheles, Culex, Mansonia, and Stegomyia are genera 
containing m ost species involved i n the transmission of protozo an and other disease -causing parasites.  
3.2 
mosquito repellent  
substance applied to deter mosquito from approaching or settling.  
3.3 
natural repellent  
repellent that contain, plant -based compounds  
3.4 
synthetic repellent  
conve ntional repellent containing chemical compounds manufactured to imitate the natural compounds  
3.5 
absorbency time  
time taken for a liquid to be dispersed into the nonwoven disposable wet wipe  
3.6 
acceptable  
recognized by  the authority  administering this st andard, or to the parties con cluding the purchase contract, as  relevant  
3.7 
length of piec e 
distance bet ween the beginning and the end of the sample in the lengthwise or machine direction  
3.8 
overall width of piece  
distance between the outermost edges of t he sample, measured perpendic ular to the longitudinal edges  
3.9 
nonwoven  
structure of text ile material, such as fibres, continuous filaments, or chopped yarns of any nature or origin, that  have been 
formed into webs by any means, and bonded together by any  means, excluding the interla cing of  yarns as in woven fabric, 
knitted fabric, laces, brai ded fabric or tufted fabric  
3.10 
mosquito repellent wipe  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  9 
 piece of soft, wet cloth or paper containing mosquito repellent   
4 Symbols and/or abbreviated terms   
DEET N, N-Diethyl -meta -toluamide or d iethy ltoluamide  
IR3535  ethyl butylacetylaminopropionate  
Picaridin /icaridin  1-(1-methylpropoxycarbonyl) -2-(2-hydroxye thyl) piperidine or 2 -(2-hydroxyethyl) -1-piperidinecarboxylic  
acid 1 -methylpropyl ester  
5 Active ingredients  
5.1 Natural repellents  
5.1.1  Active ingred ients used in natural repellents shall be plant -based  compounds which are able to deter mosquitoes 
from approaching or settling. Such shall be essential oils or any other plant extract approved by relevant authority  as 
mosquito repellents.  
5.1.2 The manuf acturer shall provide adequate data on the repellence of such ingredients.   Adequate d ata shall include  
laboratory studies showing estimation of effective dose (technical material) and estimation of  complete protection time; and 
the outcomes of field trial s showing efficacy and persistence of technical material; efficacy and persistence of formulated 
product.  
5.1.3  The manufacturer shall have adequate data justifying the safety and proportion of ingr edient(s) used in the product, 
for which cla ims are made.  
5.1.4  The essential oils used and other plant extracts in natural repellents shall b e, but not limited to:  
a) cedarwood ;  
b) tea tree ;  
c) geranium ;  
d) rosemary ;  
e) lemongrass ;  
f) citronella ;  
g) eucalyptus ;  
h) cinnamon ;  
i) neem ;  
j) peppermint;  
k) garlic;  DEAS  1119 -3: 2023  
10 © EAC 2023  – All rights reserved  
 l) lavender 
m) cloves  
n) basils  
o) jasmine;  and 
p) pyrethrum  
5.1.5  The proportion of sin gle or blended active ingredient (s) in natural repellent shall be set by the manufacturer in 
accordance with the relevant standard and records shall be availed.  
5.1.6  Pyrethrum extract s suc h as pyrethrins shall be considered in natural repellents.  
5.2 Synthetic repellents  
5.2.1  Synthetic repellents shall contain synthetic chemical compounds which are able to deter mosquitoes from 
approaching or settling on the surfac e.  
5.2.2  If a syn thetic active ingredient is blended with other active ingredient (s), either natural or synthetic, the proportion 
shall be set by the manufact urer based on scientific rese arch and records shall be availed.  
5.2.3  Synthetic repellents and their active ingre dients shall be approved and registered by relevant authority before being 
released to the market.  
6 Requirements  
6.1 General requirements  
6.1.1 The product shall constitu te a mosquito repellent that is formulated as wipes and shall be es sentially a  produc t 
which has active ingredient(s) added to a certain level.  
6.1.2 The product shall be of acceptable uniform make and finish.  
6.1.3 The product  shall be free from defects t hat might impair their appearance or serviceability (or both).  
6.1.4 When applied to the skin, the product shall have the benefit of repelling mosquitoes and shall not  have a harmful 
effect to the skin.  
6.2 Specific requirement s 
6.2.1  Active ingredients an d their content in mosquito repellent wipes shall meet the requirements  given in Tabl e 1 
when tested in accordance with the test methods specified therein  
 
 
Table 1 — Requirements for active ingredients in mosquito repellent wipes   
S/N  Characteristic  Requirement  Test method  
i  DEET a, % w/w. 4 – 30 Annex A  
ii  IR3535 a, % w/w.  7.5 – 20.07 Annex B 
iii  Picaridin a, % w/w.  5 – 20 Annex C DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  11 
 iv Pyrethrins  b, % w/w  0.3 - 0.5 Annex D 
a Applicable to synthetic mosquito repellent wipes  
b  Applicable to mosquito repellent containing extracts from pyrtherum.  
 
6.2.2 The product shall comply wit h the specific require ments given in Table 2 when t ested in accorda nce with the  test 
methods specified therein.  
Table  2 — Specific requirements  for skin applied  mosquito  repellent s wipes  
 
S/N Param eter Requirement  Test method  
i)  Fibre compos ition, % m/m  Cellulose , min.  20 ISO 1833 -1 
Others , max. 80 
ii)  Grammage, g/m2, min.  36 ISO 9073 -1 
iii)  pH 5.5 - 8.0 ISO 3071  
iv)  Absorbency time (for manufacturer), s, max.  5 Annex E 
v)  Moisture content, % m/m, min.  50 Annex F 
vi)  Size, mm  Length   
As decla red on the label   
Anne x G Width  
vii)  Breaking 
strength, N, 
min. Machine direction  Dry 60  
 
ISO 9073 -18 Wet 30 
Cross section  Dry 3.5 
Wet 2.5 
viii)  Anti-bacterial activity (A), min.  2 ISO 20743  
6.3 Microbiolog ical requirements  
Skin applied mosqu ito repell ent wipes sh all comply with the microbio logy limits specified in Table 3 when tested in 
accordance to the methods described therein  
Table  3 — Microbiological  limits for skin  applied  mosqu ito repell ent wipes  
S/N Microorganism  Requirement  Test metho d 
i Total  viable  count, cfu/g,  max.  1 000  ISO 21149  
ii Staphylococcus  aureus,  /g Not detected  ISO 22718  
iii Pseudomonas  aeruginosa,  /g Not detected  ISO 22717 
iv Escherich ia coli, cfu/g  Not detectable  ISO 21150  
v Candida  albicans , /g Not detected  ISO 18416  DEAS  1119 -3: 2023  
12 © EAC 2023  – All rights reserved  
 6.4 Biological  efficacy  
When  tested in accordance  with DEAS 1120 -1, the product  shall repel 100 % of the mosquitoes  from  approaching  or 
settling on  the surface,  within  protection  time indicated  by the manufacturer.  
7 Packaging  
The product  shall be packaged  in suitable,  well-sealed  conta iners  that shall protect  the contents  and shall not  cause any contamin ation 
or react  with the product.  DEAS  1119 -3: 2023  
© EAC  2023  – All rights  reserved  1 
 8 Labelling  
8.1 Primary  packaging  
The primary  package shall  be legibly  and indelibly  label led  in English and/or any other official la nguage (French, 
Kiswahili, etc.) used in the importing Ea st African P artner State  with the following  information  
 
a) name  of product  as “Mosqu ito repellent  wipes”;  
b) name and physical  address of the manufacturer;  
c) importer/distributo rs name,  address  (if applicab le); 
d) number  of wipes  in a pack;  
e) intended  use, as ‘for skin applicat ion”; 
f) size of wipes  in the pack;  
g) fibre or material content  and active ingredient  used;  
h) instructions  for use; 
i) storage  conditions;  
j) instructions  on disposal;  
k) country  of origin;  
l) date of  manufac ture; 
m) expiry  date 
n) batch  number;  
o) safety  caution;  and 
p) protection  time.  
8.2 Second ary packaging  
The exterior  of eac h secondary  package  shall be legibly  and indelibly  labelled  with the following  information:  
a) manufacturer’s  name and/or  registered  trademark;  
b) the words  “Mosquito  repellent wipes”;  and 
c) number  of packages.  
9 Sampling  
Sampling  shall be done  in accordance  with ISO 2859 -1. DEAS  1119 -3: 2023  
2 © EAC 2023  – All rights reserved  
 Annex  A 
(normative)  
 
Determinati on of DEET content  
A.1 General  
The sample is dissolved in carbon disulfide and the difference in abs orbance at 14 .18 μm and at 14.48 μm is 
determi ned. The quantity of meta -isomer is obtained from this value by m eans of a calibration c urve prepared 
by the u se of a reference standard.  
A.2 Apparatus  
A.2.1  Double -beam infrared spectrophotometer  
A.2.2  Two equ ivalent infra red absorption cells, with sodium chloride windows and a path length of 
approximately 0.4 mm.  
A.3 Preparation of calibrat ion curve  
A.3.1  Weigh (to the nearest 0.1 mg) into fo ur volumetric flasks sufficient amounts of the reference DEET 
standar d of known pu rity to give concentrations of approximately 20, 40, 60 and 80 g/L when dissolved in 
carbon disulfide.  
A.3.2  Fill the re ference cell with carb on disulfide and the sample cel l with each of the standard solutions in 
turn, and record the spectra . The spectru m may be scanned rapidly, except for the region 12 – 15 μm, where 
a normal speed should be used. Carry out a blank measur ement with carbon disu lfide to correct for any 
inequa lity in the paired cells and to determine whether a cell correction i s required.  
A.3.3  Measure the absorbance at 14.18 μm and at 14.48 μm and calculate the difference between these 
values, ΔA, for each of the solutions. Plot  the values of ΔA against the c oncentration (mg/L) of the meta -
isomer.  
A.3.4  If a cell correction i s required, t he value of ΔA is determined from the formula:  
ΔA = [A14.18 – A14.48] ref. - [A14.48] blank  
Where ref. = determination w ith reference standard   
blank = determination on CS2 blank  
A.4 Procedure  
Weigh (to the nearest 0.1 mg) about 0.5 g of the sample, trans fer quantitatively to a 10 mL volumetric flask, 
and make up to the mark with carbon disulfide. Measure the infrared absor ption at 14.18 μm and 14.48 μm 
using the same conditi ons as described in clause A.3.  Determine the concentration of meta -isomer by 
comp aring this value with the calibration curve. A standard sample should be run each day to check the 
calibration of the ins trument.  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  3 
 A.5 Calculati on 
DEET content (g/kg)  
 
where,   
C1 concentration (g/L) of standard DEET found from calibration curv e  
C2 concent ration (g/L) of sample taken  
P purity (g/kg) of the reference standard  
 DEAS  1119 -3: 2023  
4 © EAC 2023  – All rights reserved  
 Annex  B 
(normative)  
 
Determination of ethyl butylac etamidopropionate (IR3535).  
B.1 Outline of method  
B.1.1  Ethyl butyla cetamidopropi onate is determined by capillary gas chromatography using flame ionisation 
detection and internal standardisation.  
B.1.2  The retention time of the ethyl butylacetamidopropion ate peak of the sample solution should not 
deviate by more than 2 % f rom that of t he calibration solution.  
B.2 Reagents  
B.2.1  Acetonitrile  
B.2.2  Ethyl butylacetamidopropionate, standard of known purity  
B.2.3 Methyl tridecanoa te, internal standard  
B.2.4  Calibration solution.  
B.2.4.1  Weigh (to the nearest 0.1 mg in duplicate)  into volumet ric flasks (10 ml) about 100 mg ethyl 
butylacetamidopropionate standard (s mg) and about 100 mg methyl tridecanoate (r mg ).  
B.2.4.2  Dissolve i n acetonitrile and fill to the mark with acetonitrile (solutions C1 and C2). The solutions 
are stabl e for one wee k at room temperature.  
B.3 Apparatus  
B.3.1  Gas chromatograph equipped with a split/splitless injection and a flame ionisa tion detector  
B.3.2  Capillary column fused silica, 25  m × 0.32 (i.d.) mm, coated with CP -Sil 5 CB, film thickness: 1.2 µm  
B.3.3  Electri c integrator or data system  
B.4 Procedure  
B.4.1  Chromatographic conditions (typical)  
B.4.1.1  Column  fused  silica,  25 m × 0.32 mm (i.d.),  film t hickness: 1.2 µm, coated with 
CP-Sil 5 CB  
B.4.1.2  Injection system  
B.4.1.2.1  Injector: split injecti on 
B.4.1.2.2  Split ratio:1:50  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  5 
 B.4.1.2  Detector: Flame ionisation  
B.4.1.3  Temperatures  
B.4.1.3.1  Injection port:300 °C  
B.4.1.3.2  Detect or:310 °C  
B.4.1.3.3  Oven program  
B.4.1.3.3.1  initial:  120 °C  
B.4.1.3.3.2  Program rate: 10 °C/min  
B.4.1.3.3.3  Final: 260 ° C 
B.4.1.4  Injection volume: 5 µl  
B.4.1.5  Gas flow rates  
B.4.1.5.1  Helium  1.1 ml/min  
B.4.1.5.2  Helium (make up)  45 ml/min  
B.4.1.5.3  Hydrogen  40 ml/min  
B.4.1.6 Retention times  
B.4.1.6.1  ethyl butylacetamidopropionate: about: 10.4 min  
B.4.1.6.2  methyl tride canoate: abou t: 10.9 min  
B.5 Preparation of sample.  
B.5.1  Weigh in duplicate (to the nearest 0.1 mg) into volumetric flasks (10 ml) su fficient sample to con tain 
about 100 mg ethyl butylac etamidopropionate (w mg) and about 100 mg methyl tridecanoate (q mg).   
B.5.2  Disso lve in acetonitrile and fill to the mark with acetonitrile (solutions S1 and S2). The solutions are 
stable for one week a t room temperature . 
B.6 System equilibration  
Inject 0 .1 µl portions of the calibration solution and repeat the injections until retenti on times and calibration 
factors vary by less than 0.1 % of the mean of three successive injections.  
B.7 Determination.  
B.7.1 Inject 0.1 µl por tions of the calibration soluti on and sample solutions in the following sequence: C 1, S1, 
C2, S2,…et c. 
B.7.2  Dete rmine the peak areas and calculate the mean response factor ( f) of the calibration solution 
injections bracketing the inj ections of the sample solutions . DEAS  1119 -3: 2023  
6 © EAC 2023  – All rights reserved  
 B.8 Calculation  
B.8.1 Individual response factor 
  is calculated as follows:   
 
B.8.2  Ethyl butylacetamidopropionate shall be calculated as follows:  
 
where:  
fi individual  response  factor  
f mean  response  factor  
Hs peak  area of ethyl  butylacetamidopropionate  in the calibration  solution  
Hw peak  area of ethyl butylacetamidopropionate  in the sampl e solution  
Ir peak  area of the internal standard  in the calibration  solution  
Iq peak  area of  the internal standard  in the sample  solution  
s mass of ethyl  butylacetamidopropio nate standard  in the calibration  solution  (mg) 
r mass  of internal  standard  in the calibration  solution  (mg) 
q mass  of internal  standard  in the sample  solution  (mg) 
w mass  of sample  taken  (mg) 
P purity  of ethyl  butylacetamidop ropionate  standard  (g/kg)  
Repea tability  r 18 g/kg at 1002  g/kg active  ingredient  content  
Reproducibi lity R 18 g/kg at 1002  g/kg active  ingredient  content  
 DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  7 
 Annex C  
(normative)  
 
Determination of icaridin  
C.1 outline of method  
Icaridin is determined by capil lary gas chromatograph using in ternal standardisation and flame ionisation 
detection.  
C.2 Reagents  
C.2.1 Icaridin  reference standard with known content  
C.2.2  Dimethyl Phthalate internal standard  
C.2.3  Propan -2-ol 
C.2.4  Calibration sol ution.  
Weigh (to the n earest 0.1 mg) about 100mg icar idin reference substance ( s mg) and 100 mg dimethyl 
phthalate ( r mg) into a volume tric flask (20 ml). Fill to the mark with propan -2-ol and homogenise.  
C.3 Apparatus  
C.3.1 Gas chromatograph capable of op erating in the range 1 50 °C to 330 °C, fitted with a flame ionisation 
detector, a split injector and an autosampler  
C.3.2 Column quartz ,30 m ×0.25 mm (i.d), coated with dimethyl polysiloxane/ diphenyl polysiloxane 95/5% 
(e.g. DB5), film thickness 0.25 µm 
C.3.3 Electronic integr ator or data system  
C.4 procedu re 
C.4.1 Chromatographic conditions (typical)  
C.4.1.1  Column: Quartz, 30 m × 0.25  mm (i.d.),  coated with dimethyl polysiloxane/ diphenyl polysiloxane 
95/5% (e.g. DB5), film thickness 0.25 µm  
C.4.1.2  Injec tion system  
C.4.1.2.1  Injector: split injection  
C.4.1.2.2 Split flow:40ml/min  
C.4.1.2  Detector: Flame ionisation  
C.4.1.3  Temperatures  DEAS  1119 -3: 2023  
8 © EAC 2023  – All rights reserved  
 C.4.1.3.1  Injector :240 °C 
C.4.1.3.2  Detector:3 30 °C 
C.4.1.3.3  Oven program : 150 °C hold for 2 min, gradient: 10 °C/ min to 3 30 °C, hold for 3 min.  
C.4.1.4  Injection volume: 1 µl  
C.4.1.5  Gas flow rates  
C.4.1.5.1  Helium (carrier)  1.5 ml/min (100kp a)  
C.4.1.5.2  Hydrogen: about 30ml/min  
C.4.1.5.3  Air: about 3 00ml/min  
C.4.1.5.4  Nitrogen (makeup):  about 25ml/min  
C.4.1.6  Run time: a bout 25 min  
C.4.1.7 Retention times  
C.4.1.6.1  Dimethy l Phthalate:  about 3 min  
C.4.1.6.2  Icaridin : about 4.5 min  
C.5 Prepar ation of samp le 
Weigh (to the nearest 0.1 mg) into a volumetric flask (20 ml) sufficient sample to contain about 100 mg icaridin 
(w mg) and about 100 mg dim ethyl phthalate ( q mg). Fill to  the mark with propan -2-ol and homogenise.  
C.6 Equilibration of the s ystem  
Inject 1 µl portion of calibration solution and repeat the injections until retention times and the icaridin to the 
internal sta ndard peak area ration  vary by less than 0.5% of the mean for successive injections.  
C.7 Determination  
C.7.1 Inject in dup licate 1 µ portions of the calibration solution (C 1 and C 2) and of the sample solution (S 1, 
S2, etc.) in the following sequence:C 1, S1, S2, ...C 2).  
C.7.2 Determine the peak areas and cal culate the response factor ( f) from the calibration solutions 
bracket ing the injec tions of the sample solutions. Calculate the content of the sample solutions.  
C.8  Calculation  
C.8.1  Response factor f is calculated as follow s:  
 
C.8.2  Icaridin content shall be calculated in g/kg as follows:  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  9 
 
 
where:  
f mean  response  factor  
Hs peak  area of icaridin  in the calibration  solution  
Hw  peak area of icaridin in the sample solution  
Ir peak  area of dimethyl phthal ate in  the calibration  solution  
Iq peak  area of  dimet hyl phthalate in  the sample  solution  
s mass  of icaridin  in the calibr ation  solutio n (mg) 
r mass  of dimethyl phthalate in  the calibration  solution  (mg) 
q mass  of dimethyl phthalate  in the sample  solution  (mg) 
w mass  of sample  taken  (mg) 
P purity  of icaridin  standard  (g/kg)  
Repeatability r  12 g/kg to 14 g/kg at  986 g/kg active ingredient  content  
Reproducibility R  14 g/kg at 986 g/kg active  ingredient  content  DEAS  1119 -3: 2023  
10 © EAC 2023  – All rights reserved  
 Annex D 
(normative)  
 
Determination of total pyret hrins  
 D.1 General  
The active ingredients in pyrethru m extract may be determined using a HPLC system first by injecting a 
solution of t he analyte into the chromatograph, followed by the separation and comparison of peaks areas of 
the analytes in the sample  with that of an exter nal standard containing a known  amount of the analytes. The 
peaks are eluted in the following order:  Cinerin II, Pyrethrin II, Jasmolin II (total Pyrethrins II) and Cinerin I, 
Pyrethrin I, Jasmolin I (total Pyrethrins I).  
D.2 Reagents  
D.2.1  World pyrethrum  standard, 50 %  
D.2.2  Acetonitr ile, HPLC grade  
D.2.3  Water, HPLC grade  
D.3 Apparatus  
A liquid chroma tography Syst em equipped with an auto -sampler, a Variable Wavelength Detector (or 
equivalent) and a Column {Phenomenex, 250 x 4.6 mm L una Phenyl -Hexyl 5μ Re verse Phase (or equivalent).  
D.4 Operating conditions  
D.4.1 Flow rate: 1.5 ml/min  
D.4.2 Composition:  40:60 (%, v/ v water/acetonitrile)  
D.4.3 Elution: Isocratic  
D.4.4 Oven temperature: 40 ºC  
D.4.5 Wavelength: 240 nm  
D.4.6 Injection Vol ume: 15 μl  
D.4.7 Stop time: 22 min  
D.4.8 Post time: 1 min  
D.5 Preparation of the standard  
Weigh 20 mg of the pyrethrum sta ndard to the nearest 0.0001 g in a 100 mL volumetric flask and dilute to 
volume with acetonitrile and label it. Transfer a small porti on to a sample vial and label it accordingly.  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  11 
 D.6 Sample preparation  
In a 100 ml volumetric flask, weigh 20 mg to the nea rest 0.0001 g  of the sample to be analyzed and dilute to 
volume with Acetonitrile. Sample this solution using a vial and label it acco rdingly.  
D.7 Procedure  
After the chromatograph is stable, make a minimum of three injections for the standard solution as  well as for 
the analyte and average the area counts. The relative standard deviation between injections should be within 
2 %.  
D.8 Calculation of the % total  pyrethrins (active ingredient)  
The total p yrethrins , expressed as percent  shall be calculated as follows;  
=
  DEAS  1119 -3: 2023  
12 © EAC 2023  – All rights reserved  
 Annex E 
(normative)  
 
Determination of absorbency rate  
E.1 Apparatus  
E.1.1 Water  tub, of a depth  of at least  100 mm and maintained  at room  temperature  
E.1.2 Stopwatch , with an accuracy  of 0.2  s 
E.1.3 Cylindrical  basket,  weighing  2.7 g ± 0.3 g of height  80 mm, diameter  50 mm with square  opening  of 
15 mm to 20 mm, made  of copper  wire of 0.4 mm  diameter  
E.1.4 Analytical  balance   
E.1.5 Forceps  
E.2 Preparation  of test specimens  
Take three  test specim ens, each  of mass  at least  1 g and compo sed of a number  of pinches  of fibres  taken  
from widely  separated  parts of  the conditioned  laboratory  sample.  
E.3 Procedure  
E.3.1 Compress  the first test specimen to a volume of appr oximately 20 ml.  
E.3.2 By means of the forceps, place the test specimen lig htly on the surface of the distilled wate r and 
simultaneousl y start the stopwatch.  
E.3.3 Using the stopwatch, measure the time it takes t he basket and its contents to sink below the  surface 
of water in seconds.  
E.3.4 Record the absorption period to the nea rest 0.1 s.  
E.3.5 Repeat the test for at least three test sp ecimens.  
E.4 Calculation  
Calculate,  to the  nearest  second,  the arithmetic  mean of the  three  test results.  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  13 
 Annex F  
(normat ive) 
Determination  of moisture  content  
F.1 Principle  
A specimen  of specifie d mass  of filler material  of the nonwoven  disposable  wet wipe is dried  in an oven  at a 
specified  temperature  and the moisture  content  is determined.  
F.2 Apparatus  
F.2.1 Balance , with an accuracy  of 0.05  % of the weighed mass  
F.2.2 Sample container , waterpr oof when sealed, will be used for transfe r of analyzed mater ial and during  
weighing  
F.2.3 Oven,  well ventilated  with a temperature  of 102 °C to 105 °C 
F.3 Sample  preparation  
F.3.1 Take a sufficient number of dry sample containers, number them and take thei r masses after they are  
held open  for a short period  of time so that they will  have  the same  air pressure  as the surrounding  
atmosphere.  Then  leave  them  open  until you take the test piece.  
F.3.2 Take  five random  pieces  of the wet wipes.  The test pieces  shall weigh  5 g. 
F.3.3 If the surrounding at mosphere is hot and  humid, prevent water condensation on the internal and  
external  surfaces of  the container.  
F.3.4 Handle the test pieces g ently to prevent dirt or changes in water content. Do not touch the test pi eces  
with your bare hands. Put the test p ieces in a containe r just after taking them and close the container  
immediately.  
F.4 Procedure  
F.4.1 Dry the test pieces in an oven with a t emperature of 102 °C to 105 °C. Open the containers lid and dry  
the specime n inside the container. Open the containe r for a moment, to balance the air pressure inside the  
container with the surrounding pressure, weigh the container that holds the specimen again and calculate the  
weight  of the specimen.  
F.4.2 First cycle  of drying  will last at least  30 min. Return  the container  with the test pieces  to the oven,  for 
at least half the first cycle drying time. Take th e container out and take the mass with the t est pieces inside.  
Repeat the drying and weighing cycles. When the drying t ime on every cycle is at least half of th e total  
previous dr ying cycle times. Continue the process until the difference between two conse cutive masses does  
not exceed  0.1 % of the original  mass  of the specime n 
F.5 Calculation  
Calculate  the moisture  content  using the  following  formula  and round  the results  up to the nearest  0.1 %. DEAS  1119 -3: 2023  
14 © EAC 2023  – All rights reserved  
 
 
where  
V is the moisture  content,  in percentage weight;  
a is weight,  in grams,  of the container with  the specimen,  before  drying;  
b is weight,  in grams,  of the container  with the specimen  after drying;  and 
c is weight,  in grams,  of the container  DEAS  1119 -3: 2023  
© EAC 2023  – All rights reserved  15 
 Annex G  
(normative)  
 
Determination  of length  and width  
G.1Apparatus  
G.1.1 Steel scale, that is of a length exceeding the width of the fabric to be measured, a nd is graduated in  
centimeters  and millimeters  
G.1.2  Marking  pen 
G.2 Procedure  
G.2.1  Procedure for  width  
G.2.1.1  Lay the test sample flat and full width (without subjecting it to tension) on a plane surface and  
condition  it, in that state  for at least  24 h in accordance  with ISO 139.  
G.2.1.2  Take, to the nearest 1 mm, five measurements across the overall width or  between the innermost  
selvedg e threads (as relevant) of the conditioned test sample at approximately equal intervals throughout its  
length.  
G.2.1.3 Calculate  the arithmetic  mean  of the five  measurements  and record it  as the width  of the sample.  
G.2.2  Proc edure  for length  
G.2.2.1  Take a laboratory sample as specified in the relevant standard. Where no standard exists, take  
the laboratory  sample  as agreed  upon  between  the test laboratory  and the manufacturer  to ensure  a 
reasonable and  acceptable reliability  at a reasonable and  acceptable  confidence  level.  
G.2.2.2  Lay the laboratory sample flat and full width (without subjecting it to tension) on a p lane surface  
and condition  it in that state  for at least  24 h in accordance  with ISO 139.  
G.2.2.3  From the co nditioned laboratory sample, c ut a test specimen across the full width of the sample,  
along a datum line drawn at right angles to the selvedges and as close as poss ible to the begin ning and the  
end of  the sample.  
G.2.2.4  Take,  to the nearest  millimetre,  five measurements  (see G.2.1.2)  of the length  of the test 
specimen  at approximately  equal intervals across  its width.  
G.2.2.5  Calculate the arithm etic mean of the fiv e measurements an d record it as the length, in metres  
(accurate  to the nearest  centimetre),  of the laboratory sample  DEAS  1119 -3: 2023  
16 © EAC 2023  – All rights reserved  
 Bibli ography  
[1] RS 392 -3: 2018 , Skin applied mosquito repellents — Specification — Part 3: Wipes  
[2] US 2296 -3: 2022, Skin applied mosquit o repellents — Specification — Part 3: Wipes  
 DEAS  1119 -3: 2023    
 
© EAC 2023  – All rights reserved  
  
  