 
  
 ICS 71.100.70 
 
Reference number  
DRS 399: 2023  
© RSB 2023  
  
 RWANDA 
STANDARD    DRS 
399  
 
Second  edition  
yyyy-mm-dd 
 
Ghee  for cosmetic industry  —  
Specification  
   
DRS 399: 2023  
©RSB 2023  - All rights reserved                  ii 
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
edition  
 
 
 
 
 
 
 
 
© RSB 2023  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission from R SB. 
Requests for permission to reproduce this document should be addressed to : 
Rwanda Standards  Board  
P.O Box 7099 Kigali -Rwanda  
KK 15 Rd, 49  
Tel. +250 788303492  
Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website: www.rsb.gov.rw  
ePortal: www.portal.rsb.gov.rw   DRS 399: 2023  
iii                ©RSB 2023  - All rights reserved  
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ............................  iv 
1 Scope  ................................ ................................ ................................ ................................ ......................  1 
2 Normative references  ................................ ................................ ................................ ............................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  2 
4 Requirements  ................................ ................................ ................................ ................................ .........  2 
4.1 General requirements  ................................ ................................ ................................ ...........................  2 
4.2 Specific requirements  ................................ ................................ ................................ ...........................  3 
5 Packaging  ................................ ................................ ................................ ................................ ...............  5 
6 Labelling  ................................ ................................ ................................ ................................ .................  5 
7 Sampling  ................................ ................................ ................................ ................................ ................  5 
Annex  A (normative)  Determination of critical solution temperature  ................................ ..........................  6 
A.1 Reagent  ................................ ................................ ................................ ................................ ..................  6 
A.2 Procedure  ................................ ................................ ................................ ................................ ...............  6 
 DRS 399: 2023  
©RSB 2023  - All rights reserved                  iv 
 Foreword   
Rwanda Standard s are prepared  by Technical Committees and approved by Rwanda Standards Board (RSB) 
Board  of Directors in  accordance with the procedures of RSB, in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The main task of technical committees is to prepare national standards.  Final Draft Rwanda S tandards 
adopted by Technical committ ees are ratified  by me mbers of RSB Board of Director s for publication and 
gazettment as Rwanda Standards.  
DRS 399 was prepared by Technical Committee RSB/TC 011, Cosmetics and related products.  
In the preparation of this standard, reference was made to the following standard:  
RS 51:2012, Butter ─ Specification  
This second  edition cancels and replaces the first  edition RS  399: 2019 , of which has been technically 
revised.  
Committee membership  
The following organizations were represented on the Technical Committee on Cosmetics and related products  
(RSB/TC 011) in the preparation of this standard.  
ALYVO RWANDA Ltd  
Beauty Makers Association (BMA)  
GAKO Organic Farming Training Center (GOFTC/Rwanda All Green Investment Ltd)  
J&K 
KAN-HAN Ltd  
MORIJA Cosmetics/Morija Supply Ltd  
National Industrial Research and Development Agency (NIRDA)  
PHARMACIE NOVA  
Rwanda Agriculture Livestock Inspection and Certification Services (RALIS)  
Rwanda Biomedical Center (RBC)  DRS 399: 2023  
v                ©RSB 2023  - All rights reserved  
 SULFO Rwanda Industries  
Trust Industries Ltd  
University of Rwanda/College of Science and Technology (UR/CST)  
Rwanda Forensic Laboratory (RFL)  
Rwanda Inspectorate, Competition and Consumer Protection Authority (RICA)  
Rwanda  Standards Board  (RSB) – Secretariat  DRS 399: 2023  
i Error! Reference source not found.  
 Ghee for cosmetic industry  — Specification  
1 Scope   
This Draft Rwanda  Standard s pecifies the requirements, sampling and test methods for ghee used in cosmetic 
industry.  This standard does not cover the ghee  for which therapeutic claims are made . 
2 Normative references   
The following documents are referred to in the text in such a way that some or all of  their content constitutes 
requirements of this document.  For dated references, only the edition  cited applies. For undated references, 
the latest edition of the referenced document (including any amendments) applies.  
RS EAS 346, Labelling of cosmetics — General requirements  
RS EAS 846, Glossary of terms relating to the cosmetic industry  
RS EAS 847 – 2, Cosmetics — Analytical methods — part 2: Determination of moisture content and volatile 
matter content  
RS EAS 847 -5, Cosmetics — Analytical methods — Part 5: Determination of unsaponifiable matter  
RS EAS 847 -7, Cosmetics — Analytical methods — Part 7: Determination of specific gravity  
RS EAS 847 -9, Cosmetics — Analytical methods — Part 9: Determination of colour  
RS EAS 847 -10, Cosmetics — Analytical methods — Part 10: Determination of acetyl value and hydroxyl 
value  
RS EAS 847 -12, Cosmetic — Analytic methods — Part 12: Determination of flash point by Pensky — Martens 
Closed Cap Tester   
RS EAS 847-13, Cosmetic — Analytic methods — Part 13: Determination of rancidity  
RS EAS 847 -16, Cosmetic — Analytic methods — Part 16: Determination of lead, mercury and arsenic 
content  
RS ISO 22717, Cosmetics — Microbiology — Detection of Pseudomonas aeruginosa   
 RS ISO 2271 8, Cosmetics — Microbiology — Detection of Staphylococcus aureus  
RS ISO 18416 , Cosmetics — Microbiology — Detection of Candida albicans  
 
RS ISO 660, Animal and vegetable fats and oils — Determination of acid value and acidity  
RS ISO 663, Animal and vegetable fats and oils — Determination of insoluble impurities content  
RS ISO 3657, Animal and vegetable fats and oils — Determination of saponification value  
RS ISO 3961, Animal and vegetable fats and oils — Determination of iodine value  
RS ISO 6320, Animal and vegetable fats and oils — Determination of refractive index  
RS 278, Cosmetics — Methods of sampling  
3 Terms and definitions   
For the purposes of this standard, the terms and definitions given in RS EAS 846 and the following apply . 
3.1 
ghee  
product  exclusively obtained from milk, cream or butter, by means of heat processes which result in almost 
total removal of water and non -fat solids, with an especially developed flavour and physical structure  
3.2 
butter  
fatty product derived exclusively from milk and products obtained from milk, principally in the form of an 
emulsion of the type water -in-oil 
4 Requirements  
4.1 General requirements  
4.1.1  The product shall be obtained from cow milk, cream or butter, by means of heat processes which 
result in almost total removal of water and non -fat solids, with an especially developed flavour and physical 
structure.  
4.1.2 When examined visually, the product shall be free from:   
3                ©RSB 2023  - All rights reserved  
 a) admixture with other oils;  
b) free from sediments and other foreign matter;  
c) separated water;  
d) added colouring and flavouring substances; and  
e) insects, worms, fungal infestation, rodent contaminations.  
 
4.2 Specific requirements  
The product shall comply with the requirements given in Table 1 when tested in accordance with methods 
specified therein.  
Table 1 — Specific requirements for ghee for cosmetic industry  
S/N Parameters  Requirements  Test method  
i. i Moisture content, % m/m, max.  0.2 RS EAS 847 -2 
ii. i
i Insoluble impurities, % m/m, max.  0.25 RS ISO 663  
iii. i
i
i Colour in a 1" cell on the Lovibond 
scale, expressed as Y + 5R, max. 
deepness  4.0 RS EAS 847 -9 
iv. i
v Refractive index at 20 oC, range  1.4530 – 1.4590 RS ISO 6320  
v. v Specific gravity at 20 oC, range  0.910 – 0.913 RS EAS 847 -7 
vi. v
i Saponification value, range  225 - 235 RS ISO 3657  
vii. v
i
i Iodine value, range  26 - 40 RS ISO 3961  
viii. v
i
i
i Acid value, max.  1.0 RS ISO 660  
ix. i
x Unsapnifiable matter, % m/m, max.  1.5 RS EAS 847 -5  
 x. x Acetyl value, min.  143 RS EAS 847 -10 
xi. x
i Flash point, oC (Pensky Martens 
closed), min.  250 RS EAS 847 -12 
xii. x
i
i Test for rancidity  Shall be free from 
rancidity  RS EAS 847 -13 
xiii. x
i
i
i Critical solution temperature,  oC 
max.  0 Annex A 
4.2.2 The products shall comply with the limits for heavy metal contaminants in accordance with Table 2, 
when tested in accordance with the methods specified therein.  
 
Table 2 — Limits for heavy metal contaminants for ghee for cosmetic industry  
S/N Characteristics  Maximum limit (mg/kg)  Test method  
i. i Lead  10 
RS EAS 847 -16 ii. i
i Arsenic  2 
iii. i
i
i Mercury  2 
-  The total amount of heavy metals as lead, mercury and arsenic, in combination, in the finished 
product should not exceed 10 mg/kg.  
- The heavy metals including lead, mercury and arsenic may be as a result of contamination 
during processing and should not be deliberately added as ingredients.  
4.2.3 The products shall also comply with the microbiological limits given in Table 3 when tested in 
accordance with the methods described therein.  
Table 3 — Microbiological limits for ghee cosmetic in cosmetic industry  
S/N Microorganism  Maximum l imit 
 Test method  
i.  Total viable count for aerobic mesophyllic micro -organisms  per g . 100 RS ISO 6887 -1 
ii.  Pseudomonas aeruginosa  Not detected in 0.5 g  RS ISO 22717  
iii.  Staphylococcus aureus  RS ISO 22718  
iv.  Candida albicans  RS ISO 18416   
5                ©RSB 2023  - All rights reserved  
 5 Packaging  
The product shall be packaged in suitable well -sealed containers that shall protect the contents and shall not 
cause any contamination or react with the products.  
6 Labelling  
6.1 In addition to the requirements of RS EAS 346, the Material Safety Data Sheet shall be provided.  
6.2 The phrase ‘For external use only’ shall be conspicuously marked (either printed on the label affixed to 
the container, or lithographed, or stencilled thereon with indelible ink).  
7 Sampling  
Sampling shall be done  in accordance with RS 278.  
 
  
 Annex  A 
(normative)  
 
Determination of critical solution temperature  
A.1 Reagent   
The reagent shall be prepared by diluting ethyl  alcohol or rectified spirit with distilled water till the relative 
density of the mixture at 15.5 oC is 0.8303 ± 0.0001, when compared with distilled water at the same 
temperature. De -natured alcohol shall not be used for this test.  
A.2 Procedure  
Mix in a test tube, 1.0 g of the oil, with 4.15 times its mass of the reagent. Upon examination, the solution thus 
obtained shall be perfectly clear at 20 oC and shall remain clear when cooled and maintained for 5 minutes at 
a temperature of 0 oC. 
   
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