COMMISSION IMPLEMENTING REGUL ATION (EU) 2020/1764 
of 25 November 2020 
concer ning the author isation of disodium 5'-inosinate produced by fermenta tion with 
Cor ynebacter ium stationis KCCM 80161 as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Havi ng regar d to the Treaty on the Functioning of the European Union, 
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003, an application was submitted for the author isation 
of disodium 5'-inosinate produced by fermentation with Corynebacter ium stationis KCCM 80161. This application 
was accompanied by the particulars and documents required under Article 7(3) of Regulation (EC) No 1831/2003. 
(3) This application concer ns the author isation of disodium 5'-inosinat e produced by fermentation with Corynebacter ium 
stationis KCCM 80161 as a feed additive for all animal species. The applicant requested this additive to be classif ied in 
the additive cate gory ‘sensor y additives’. 
(4) The applicant request ed the feed additive to be author ised for use also in wate r for drinking. Howe ver, Regulation 
(EC) No 1831/2003 does not allow the author isation of ‘flavouring compo unds ’ for use in water for drinking. 
Theref ore, the use of disodium 5'‐inosinat e produced by fermentation with Corynebacter ium stationis KCCM 80161 in 
water for drinking should not be allowe d. The fact that the additive is not author ised for use as a flavouring in wate r 
for drinking does not preclude its use in compo und feed administ ered via water . 
(5) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 7 May 2020 (2) that, under the 
proposed conditions of use, disodium 5'‐inosinate produced by fermentation with Corynebacter ium stationis KCCM 
80161 does not have adverse effects on animal health, consumer health or the environment. The Author ity 
concluded in the opinion that the additive is not toxic by inhalation, not irritant to skin or eyes and is not a dermal 
sensitiser . The Authority also concluded that the effect of disodium 5'-inosinate produced by fermentation with 
Corynebacter ium stationis KCCM 80161 to increase the taste of food is well proven , and theref ore, no further 
demonstration of its efficacy in feed is necessar y. The Author ity does not consider that there is a need for specifi c 
requirements of post-market monitori ng. It also verified the repor t on the method of analysis of the feed additives 
in feed submitte d by the Reference Laboratory set up by Regulation (EC) No 1831/2003. 
(6) The assessment of disodium 5'-inosinate produced by fermentation with Corynebacter ium stationis KCCM 80161 
shows that the conditions for author isation, as provid ed for in Article 5 of Regulation (EC) No 1831/2003, are 
satisfied. According ly, the use of disodium 5'-inosinat e produced by fermentation with Corynebacter ium stationis 
KCCM 80161 should be author ised as specifi ed in the Annex to this Regulation. 
(7) Restr ictions and conditions should be provid ed for to allow better control. In particular , a recommended content 
should be indicated on the label of the feed additive. Where such content is exceeded, certain information should be 
indicated on the label of premixtures. 
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Jour nal 2020;18(5):6140. EN Offi cial Jour nal of the European Union 26.11.2020                                                                                                                                       L 397/21   HAS ADOPTED THIS REGUL ATION: 
Article 1 
The substance specified in the Annex, belonging to the additive cate gory ‘sensor y additives’ and to the functional group 
‘flavouring compo unds ’, is author ised as a feed additive in animal nutr ition subject to the conditions laid down in that 
Annex. 
Article 2 
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member States. 
Done at Brussels, 25 November 2020.  
For the Commission 
The President 
Ursula VON DER LEYEN     EN Offi cial Jour nal of the European Union L 397/22                                                                                                                                       26.11.2020   ANNEX 
Identification 
number of the 
additive Name of 
the holder 
of 
author isa-
tion Additive Compo sition, chemical formula, descr iption, 
analytical method Species or 
category of 
animal Maximum 
age Minimum 
content Maximum 
content 
Other provisions End of period of 
author isation mg of active 
substance/kg of 
compl ete feedingstuff 
with a moisture conten t 
of 12 % 
Categor y: Sensor y additiv es 
Functional group: Flavour ing compounds 
2b631i - Disodium  
5'-inosinate Additive composition 
Disodium 5'-inosinate 
Char acter isation of the active substance 
Disodium 5'-inosinate 
Produced by fermentation with 
Corynebacter ium stationis (KCCM 80161) 
Purity: ≥ 97 % (% assa y) 
Chemical formula: 
C10H11N4Na 2O8P·7.5H 2O 
CAS number 4691-65-0 
Method of analysis (1) 
For the identification of disodium 5'- 
inosinate in the feed additive: FAO JECF A 
monographs ‘disodium 5'-inosinat e’ and 
‘disodium 5′-r ibonucleotides’. 
For the determination of disodium 5'- 
inosinate (IMP) in the feed additive and 
flavouring premixtures: high 
performa nce liquid chromat ography 
coupled to UV detec tion (HPL C-UV) All animal 
species - - - 1. The additive shall be incor porat ed 
into the feed in the form of a 
premixture. 
2. In the directions for use of the  
additive and premixture, the 
stora ge conditions and the 
stability to heat treatment shall be 
indicated . 
3. On the label of the additive the 
follo wing shall be indicated: 
‘Recommended maximum 
conte nt of the active substance 
alone or in combination with 
other author ised disodium  
5′-r ibonucleotides shall be:  
50 mg/kg of compl ete 
feedingstuff with a moisture 
conte nt of 12 % ’. 
4. The functional group, the  
identif ication number , the name 
and the added amount of the 
active substance shall be indicated 
on the label of the premixtures, if 
the follo wing content of the 
active substance in compl ete 
feedingstuff with a moisture 
conte nt of 12 % is exceeded:  
50 mg/kg. 16.12.2030 
(1) Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts   EN Offi cial Jour nal of the European Union 26.11.2020                                                                                                                                       L 397/23   