Draft for  The Use Restrictions and Labeling 
Requirements of trans -resveratrol  Produced by 
Genetically Modified  Saccharomyces cerevisiae  
strain  EFSC4687  as a Food Ingredient  
MOHW Food No.1 11130 4020 , 23 February  2023 
Article 1  
The regulation  is prescribed in accordance with the provisions of Paragraph 2 
of Article 1 5-1 and Subparagraph 10 of Paragraph 1 of Article 22 of the Act 
Governing Food Safety and Sanitation . 
 
Article 2 
The trans -resveratrol  referred to herein is produced by fermentation process 
using genetically modified Saccharomyces cerevisiae  strain  EFSC4687 . The 
trans -resveratrol  powder can be purified from the fermentation via a sequence 
of purification steps including ultrafiltration , wash ing with water , pH 
adjustment with sodium hydroxide , diafiltration , precipitation  with sulfuric 
acid, concentration , filtration , dissolution  with ethanol , anion-exchange resin , 
carbon treatment , crystallization with water, filtration  and drying  etc. The final 
trans -resveratrol  powder shall not contain any genetically modified 
microorganism and its transgenes . 
 
Article 3  
For use as a food ingredient,  the trans -resveratrol  produced by  S. cerevisiae  
strain  EFSC4687  shall comply with  the following requirements : 
1. Specifications listed  in the A ppendix . 
2. Can only be used for  food supplements  intended for adult population , and 
the daily intake  shall not exceed  150 mg. 
3. The container or external packaging of  trans -resveratrol  produced by  S. 
cerevisiae  strain  EFSC4687  shall be display ed one of the following information: “The trans -resveratrol  is produced by genetically modified 
microorganism ” or “The trans -resveratrol  is produced by genetically 
modified microorganism, but ultimately does not contain any genetically 
modified microorganism and its transgenes ”. Final products containing 
trans -resveratrol  as one of its ingredients are exempted from this labeling 
requiremen t. 
 
Article 4  
The labelling of food products containing the  trans -resveratrol  shall bear the 
following warning statement s: This product contains trans -resveratrol , people 
under eighteen years old, pregnant women  and lactating women shall avoid 
consuming . People using  medicines sh all only consume the  product under 
medical supervision . 
 
  Appendix  
Specifications of the trans -resveratrol produced by genetically modified  
Saccharomyces cerevisiae  strain  EFSC4687  
Chemical name:  5-[(E)-2-(4-hydroxyphenyl)ethenyl ]benzene -1,3-diol 
Chemical formula:  C14H12O3 
Molecular weight:  228.25 Da  
CAS No:  501-36-0 
Appearance:  Off-white to slight yellow powder  
Purity   
trans -resveratrol : ≥ 98%  (dry weight basis ) 
Genetically modified microorganisms 
and their transgenes  detection : Negative ( Detected by  polymerase chain 
reaction, PCR)  
Ash: ≤ 0.5%  
Water : ≤ 3.0%  
Heavy Metals   
Lead : < 1 ppm  
Arsenic : < 1.5 ppm  
Cadmium : < 1 ppm  
Mercury : < 0.1 ppm  
 