ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 1/11COMMISSION IMPLEMENTING REGUL ATION (EU) 2024/251 
of 16 Januar y 2024
concer ning the renewal of the author isation of the preparations of Lactiplantibacillus plantar um 
CNCM I-3235, Lactiplantibacillus plantar um DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM 
I-3237, Pediococcus acidilactici DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, 
Acidipropion ibacter ium acidipropionici CNCM I-4661, Lentilactobacillus buch neri 
NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilg ardii CNCM I-4785 and Lentilactobacillus 
buchneri CNCM I-4323/NCIMB 40788 as feed additiv es for all animal species, amending 
Implementing Regulations (EU) No 1065/2012, (EU) No 1119/2012, (EU) No 1113/2013 and (EU) 
No 304/2014 and repealing Implementing Regulations (EU) No 990/2012 and (EU) 2019/764 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting and renewing such author isation.
(2) The preparations of Lactiplantibacillus plantar um (previously taxonomically identif ied as Lactobacillus plantar um) 
CNCM I-3235 and of Lactiplantibacillus plantar um DSM 11672/CNCM I-3736 (previously taxonomically identif ied 
as Lactobacillus plantar um CNCM MA 18/5U) were author ised for a period of 10 years as feed additives for all animal 
species by Commission Imple menting Regulation (EU) No 1065/2012 (2).
(3) The preparation of Pediococcus acidilactici CNCM I-3237 was author ised for a period of 10 years as a feed additive for 
all animal species by Commission Implementing Regulation (EU) No 304/2014 (3).
(4) The preparations of Pediococcus acidilactici DSM 11673/CNCM I-4622 (previously taxonomically identif ied as 
Pediococcus acidilactici CNCM MA 18/5M – DSM 11673) and of Pediococcus pentosaceus NCIMB 12455 were 
author ised for a period of 10 years as feed additives for all animal species by Commission Implementing Regulation 
(EU) No 1119/2012 (4).
(1)OJ L 268, 18.10.2003, p. 29.
(2)Commission Implementing Regulation (EU) No 1065/2012 of 13 November 2012 concer ning the author isation of preparations of 
Lactobacillus plantar um (DSM 23375, CNCM I-3235, DSM 19457, DSM 16565, DSM 16568, LMG 21295, CNCM MA 18/5U, NCIMB 
30094, VTT E-78076, ATCC PTSA-6139, DSM 18112, DSM 18113, DSM 18114, ATCC 55943 and ATCC 55944) as feed additives 
for all animal species (OJ L 314, 14.11.2012, p. 15).
(3)Commission Implementing Regulation (EU) No 304/2014 of 25 March 2014 concer ning the author isation of the preparations of 
Entero coccus faecium NCIMB 10415, Entero coccus faecium DSM 22502 and Pediococcus acidilactici CNCM I-3237 as feed additives for all 
animal species (OJ L 90, 26.3.2014, p. 8).
(4)Commission Implementing Regulation (EU) No 1119/2012 of 29 November 2012 concer ning the author isation of preparations of 
Pediococcus acidilactici CNCM MA 18/5M DSM 11673, Pediococcus pentosaceus DSM 23376, NCIMB 12455 and NCIMB 30168, 
Lactobacillus plantar um DSM 3676 and DSM 3677 and Lactobacillus buchner i DSM 13573 as feed additives for all animal species 
(OJ L 330, 30.11.2012, p. 14).
Offi cial Jour nal  
of the European Union EN 
L series    
2024/251  17.1.2024ELI: http://data.europa.eu/eli/reg_im pl/2024/251/oj 2/11(5) The preparation of Acidipr opionibacter ium acidipropionici CNCM I-4661 (previously taxonomically identif ied as 
Propionibacter ium acidipr opionici CNCM MA 26/4U) was author ised for a period of 10 years as a feed additive for all 
animal species by Commission Implementing Regulation (EU) No 990/2012 (5).
(6) The preparation of Lentilactobacillus buchner i (previously taxonomically identif ied as Lactobacillus buchner i) 
NCIMB 40788/CNCM I-4323 was author ised for a period of 10 years as a feed additive for all animal species by 
Commission Implementing Regulation (EU) No 1113/2013 (6).
(7) The preparation of Lentilactobacillus hilgardii (previously taxonomically identified as Lactobacillus hilgardii) CNCM 
I-4785 and Lentilactobacillus buchner i (previously taxonomically identif ied as Lactobacillus buchner i) CNCM I-4323/ 
NCIMB 40788 was author ised for a period of 10 years as a feed additive for all animal species by Commission 
Implementing Regulation (EU) 2019/764 (7).
(8) In accordance with Article 14(1) of Regulation (EC) No 1831/2003, an application was submitted for the renewal of 
the author isation of the preparations of Lactiplantibacillus plantar um CNCM I-3235, Lactiplantibacillus plantar um 
DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM I-3237, Pediococcus acidilactici DSM 11673/CNCM I-4622, 
Pediococcus pentosaceus NCIMB 12455, Acidipr opionibacter ium acidipropionici CNCM I-4661, Lentilactobacillus buchner i 
NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchner i CNCM 
I-4323/NCIMB 40788 as feed additives for all animal species, requesting the additives to be classifie d in the additive 
catego ry ‘techn ological additives ’ and in the functional group ‘silag e additives’. That application was accompan ied by 
the particulars and documents required under Article 14(2) of that Regulation.
(9) The European Food Safe ty Author ity (‘the Authority’) concluded in its opinion of 31 Januar y 2023 (8)that the 
abov ementioned preparations remain safe for all animal species, the consumers and the environment under the 
existing conditions of author isation. It also concluded that the additives should be considered as respirato ry 
sensitisers. In the absence of data, no conclusions could be drawn on the skin sensitisation, and skin and eye 
irritancy potential of the additives, with the exce ption for Pediococcus acidilactici DSM 11673/CNCM I-4622 that is 
considered non-ir ritant to skin and eyes. It also indicated that there is no need for assessing the efficacy of the 
additives in the conte xt of the renewal of the author isation.
(10) The Reference Laborator y set up by Regulation (EC) No 1831/2003 considered that the conclusions and 
recommendations reac hed in the assessments carried out regar ding the method of analysis of the preparations of 
Lactiplantibacillus plantar um CNCM I-3235, Lactiplantibacillus plantar um DSM 11672/CNCM I-3736, Pediococcus 
acidilactici CNCM I-3237, Pediococcus acidilactici DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, 
Acidipro pionibacter ium acidiprop ionici CNCM I-4661, Lentilactobacillus buchner i NCIMB 40788/CNCM I-4323 and 
Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchner i CNCM I-4323/NCIMB 40788 as feed additives 
in the conte xt of the previous author isations are valid and applicable for the current application. In accordance with 
Article 5(4), point (c) of Commission Regulation (EC) No 378/2005 (9), an evaluation repor t of the Reference 
Laboratory is theref ore not required.
(5)Commission Implementing Regulation (EU) No 990/2012 of 25 October 2012 concer ning the author isation of a preparation of 
Propionibacter ium acidipropio nici (CNCM MA 26/4U) as a feed additive for all animal species (OJ L 297, 26.10.2012, p. 15).
(6)Commission Implementing Regulation (EU) No 1113/2013 of 7 November 2013 concer ning the author isation of preparations of 
Lactobacillus plantar um NCIMB 40027, Lactobacillus buchner i DSM 22501, Lactobacillus buchner i NCIMB 40788/CNCM I-4323, 
Lactobacillus buchner i LN 40177/A TCC PTA-6138, and Lactobacillus buchner i LN 4637/A TCC PTA-2494 as feed additives for all animal 
species (OJ L 298, 8.11.2013, p. 29).
(7)Commission Implementing Regulation (EU) 2019/764 of 14 May 2019 concer ning the author isation of a preparation of Lactobacillus 
hilgardii CNCM I-4785 and Lactobacillus buchner i CNCM I-4323/NCIMB 40788 as a feed additive for all animal species (OJ L 126, 
15.5.2019, p. 1).
(8)EFSA Journal 2023;21(2):7865.
(9)Commission Regulation (EC) No 378/2005 of 4 March 2005 on detailed rules for the implement ation of Regulation (EC) 
No 1831/2003 of the European Parliament and of the Council as regards the duties and tasks of the Community Reference 
Laborato ry concer ning applications for author isations of feed additives (OJ L 59, 5.3.2005, p. 8).OJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 3/11(11) In view of the above, the Commission considers that the preparations of Lactiplantibacillus plantar um CNCM I-3235, 
Lactiplantibacillus plantar um DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM I-3237, Pediococcus acidilactici 
DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, Acidipropionibacter ium acidipropionici CNCM 
I-4661, Lentilactobacillus buchner i NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and 
Lentilactobacillus buchner i CNCM I-4323/NCIMB 40788 satisfy the conditions provi ded for in Article 5 of Regulation 
(EC) No 1831/2003. According ly, the author isation of those additives should be renewed. In addition, the 
Commission considers that appropr iate protective measures should be taken to prevent adverse effects on the 
health of the users of the additive.
(12) As a consequence of the renewal of the author isation of the preparations of Lactiplantibacillus plantar um CNCM 
I-3235, Lactiplantibacillus plantar um DSM 11672/CNCM I-3736, Pediococcus acidilactici CNCM I-3237, Pediococcus 
acidilactici DSM 11673/CNCM I-4622, Pediococcus pentosaceus NCIMB 12455, Acidipr opionibacter ium acidipropionici 
CNCM I-4661, Lentilactobacillus buchner i NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 
and Lentilactobacillus buchner i CNCM I-4323/NCIMB 40788 as feed additives, Implementing Regulations (EU) 
No 1065/2012, (EU) No 1119/2012, (EU) No 1113/2013 and (EU) No 304/2014 should be amended and 
Implementing Regulations (EU) No 990/2012 and (EU) 2019/764 should be repealed.
(13) Since safet y reasons do not require the immediat e application of the modif ications to the conditions of the 
author isation of the preparations of Lactiplantibacillus plantar um CNCM I-3235, Lactiplantibacillus plantar um 
DSM 11672/CNCM I-3736, Pediococcus acidilactici DSM 11673/CNCM I-4622, Acidipropionibacter ium acidipr opionici 
CNCM I-4661, Lentilactobacillus buchner i NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 
and Lentilactobacillus buchner i CNCM I-4323/NCIMB 40788, in relation to the taxonomical change of the 
microorg anisms contained therein, it is appropr iate to provid e for a transitional period for interest ed parties to 
prepare themselves to meet the new requirements resulting from the renewal of the author isations.
(14) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Renewal of author isation
The author isation of the preparations specifi ed in the Annex, belonging to the additive categor y ‘technological additives’ 
and to the functional group ‘silage additives ’, are renewed subject to the conditions laid down in that Annex.
Article 2
Amendment to Implementing Regulation (EU) No 1065/2012
In the Annex to Implementing Regulation (EU) No 1065/2012, entr y 1k20717 on Lactobacillus plantar um (CNCM I-3235) 
and entr y 1k20722 on Lactobacillus plantar um (CNCM MA 18/5U) are deleted.
Article 3
Amendment to Implementing Regulation (EU) No 1119/2012
In the Annex to Implementing Regulation (EU) No 1119/2012, entr y 1k2104 on Pediococcus acidilactici CNCM MA 18/5M 
DSM 11673 and entr y 1k2106 on Pediococcus pentosaceus NCIMB 12455 are delet ed.OJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/251/oj 4/11Article 4
Amendment to Implementing Regulation (EU) No 1113/2013
In the Annex to Implemen ting Regulation (EU) No 1113/2013, entr y 1k20739 on Lactobacillus buchner i 
NCIMB 40788/CNCM I-4323 is delet ed.
Article 5
Amendment to Implementing Regulation (EU) No 304/2014
In the Annex to Implementing Regulation (EU) No 304/2014, entr y 1k21009 on Pediococcus acidilactici CNCM I-3237 is 
delet ed.
Article 6
Repeal of Implementing Regulations (EU) No 990/2012 and (EU) 2019/764
Implementing Regulations (EU) No 990/2012 and (EU) 2019/764 are repealed.
Article 7
Transitional measures
The preparations of Lactiplantibacillus plantar um CNCM I-3235, Lactiplantibacillus plantar um DSM 11672/CNCM I-3736, 
Pediococcus acidilactici DSM 11673/CNCM I-4622, Acidipro pionibacter ium acidipr opionici CNCM I-4661, Lentilactobacillus 
buchner i NCIMB 40788/CNCM I-4323 and Lentilactobacillus hilgardii CNCM I-4785 and Lentilactobacillus buchner i CNCM 
I-4323/NCIMB 40788 specified in the Annex, and feed containing them which are produced and labelled before 
6 Febr uary 2025 in accordance with the rules applicable before 6 Febr uary 2024 may continue to be placed on the mark et 
and used until the existing stoc ks are exhausted.
Article 8
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 16 Januar y 2024 .
For the Commission
The President
Ursula VON DER LEYENOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 5/11ANNEX 
Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k20717 Lactiplantiba -
cillus 
plantar um 
CNCM I-3235Additive composition
Preparation of Lactiplantibacillus 
plantar um CNCM I-3235 containing 
a minimum of 5 × 1010 CFU/g additive
Char acter isation of the active substance
Viable cells of Lactiplantibacillus 
plantar um CNCM I-3235
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15787
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 2 × 107 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/251/oj 6/11
Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k20722 Lactiplantiba -
cillus 
plantar um 
DSM 
11672/CNC -
M I-3736Additive composition
Preparation of Lactiplantibacillus 
plantar um DSM 11672/CNCM 
I-3736 containing a minimum of 2 
× 10 10 CFU/g additive
Char acter isation of the active substance
Viable cells of Lactiplantibacillus 
plantar um DSM 11672/CNCM 
I-3736
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15787
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 1 × 108 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 7/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k21009 Pediococcus 
acidilactici 
CNCM I-3237Additive composition
Preparation of Pediococcus acidilactici 
CNCM I-3237 containing a 
minimum of 1 × 1010 CFU/g additive
Char acter isation of the active substance
Viable cells of Pediococcus acidilactici 
CNCM I-3237
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15786
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 5 × 107 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment6 Febr uary 2034
1k2104 Pediococcus 
acidilactici 
DSM 
11673/CNC -
M I-4622Additive composition
Preparation of Pediococcus acidilactici 
DSM 11673/CNCM I-4622 
containing a minimum of 3 × 109 
CFU/g additive.
Char acter isation of the active substance
Viable cells of Pediococcus. acidilactici 
DSM 11673/CNCM I-4622
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15786
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 3 × 107 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin and 
breathing prot ective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/251/oj 8/11
Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k2106 Pediococcus 
pentosaceus 
NCIMB 
12455Additive composition
Preparation of Pediococcus pentosaceus 
NCIMB 12455 containing a 
minimum of 3 × 109 CFU/g additive
Char acter isation of the active substance
Viable cells of Pediococcus pentosaceus 
NCIMB 12455
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15786
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 3 × 107 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 9/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k2111 Acidipro pioni -
bacter ium 
acidiprop ionici 
CNCM I-4661Additive composition
Preparation of Acidipropionibacter ium 
acidiprop ionici CNCM I-4661 
containing a minimum of 1 × 108 
CFU/g additive
Char acter isation of the active substance
Viable cells of Acidipropionibacter ium 
acidiprop ionici CNCM I-4661
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15787
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other microorg anisms 
as silage additives: 1 × 108 CFU/kg fresh mat erial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/251/oj 10/11
Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k20739 Lentilactobacil -
lus. buchner i 
NCIMB 
40788/CNC -
M I-4323Additive composition
Preparation of Lentilactobacillus 
buchner i NCIMB 40788/CNCM 
I-4323 containing a minimum of 3 
× 109 CFU/g additive
Char acter isation of the active substance
Viable cells of Lentilactobacillus 
buchner i NCIMB 40788/CNCM 
I-4323
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15787
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other micro-organ ­
isms as silage additives: 1 × 108 CFU/kg fresh ma­
terial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/251/oj 11/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k20757 Lentilactobacil -
lus hilgardii 
CNCM I-4785 
and 
Lentilactobacil -
lus. buchner i 
CNCM 
I-4323/ 
NCIMB 
40788Additive composition
Preparation of Lentilactobacillus 
hilgardii CNCM I-4785 and 
Lentilactobacillus buchner i CNCM 
I-4323/NCIMB 40788 containing a 
minimum of 1,5 × 1011 CFU/g 
additive (ratio of 1:1)
Char acter isation of the active substance
Viable cells of Lentilactobacillus 
hilgardii CNCM I-4785 and 
Lentilactobacillus buchner i CNCM 
I-4323/NCIMB 40788
Analytical method (1)
— Enumeration: Spread plate 
method: EN 15787
— Identif ication: Pulsed- Field Gel 
Electrophoresis (PFGE) – CEN/TS 
17697 or DNA sequencing meth ­
odsAll animal 
species— — — 1.In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2.Minimum content of the additive when used 
without combination with other micro-organ ­
isms as silage additives: 3 × 108 CFU/kg (L. hilgar­
dii CNCM I-4785 and L. buchner i CNCM I-4323/ 
NCIMB 40788 in ratio of 1:1) of easy and mod ­
erately difficult to ensile fresh mater ial(2).
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress pote ntial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin, eye 
and breathing protective equipment.6 Febr uary 2034
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en
(2)Easy to ensile forag e: > 3 % soluble carbohydrat es in fresh mat erial. Moderately difficult to ensile forage: 1,5–3,0 % soluble carbohydrat es in the fresh material . Commission Regulation (EC) No 429/2008 of 
25 Apr il 2008 on detailed rules for the implem entation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and 
the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).OJ L, 17.1.2024 EN  